alfalfa mosaic virus

Summary

Summary: The type species of the genus ALFAMOVIRUS that is non-persistently transmitted by aphids.

Top Publications

  1. Venkatachalan S, Bushman J, Mercado J, Sancar F, CHRISTOPHERSON K, Boileau A. Optimized expression vector for ion channel studies in Xenopus oocytes and mammalian cells using alfalfa mosaic virus. Pflugers Arch. 2007;454:155-63 pubmed
    ..Furthermore, we found that expression using pUNIV was significantly enhanced in oocytes and was remarkably long-lasting in both systems...
  2. Neeleman L, Linthorst H, Bol J. Efficient translation of alfamovirus RNAs requires the binding of coat protein dimers to the 3' termini of the viral RNAs. J Gen Virol. 2004;85:231-40 pubmed
    The coat protein (CP) of Alfalfa mosaic virus (AMV) is required to initiate infection by the viral tripartite RNA genome whereas infection by the tripartite Brome mosaic virus (BMV) genome is independent of CP...
  3. Huang M, Jongejan L, Zheng H, Zhang L, Bol J. Intracellular localization and movement phenotypes of alfalfa mosaic virus movement protein mutants. Mol Plant Microbe Interact. 2001;14:1063-74 pubmed
    Thirteen mutations were introduced in the movement protein (MP) gene of Alfalfa mosaic virus (AMV) fused to the green fluorescent protein (GFP) gene and the mutant MP-GFP fusions were expressed transiently in tobacco protoplasts, tobacco ..
  4. Sanchez Navarro J, Miglino R, Ragozzino A, Bol J. Engineering of alfalfa mosaic virus RNA 3 into an expression vector. Arch Virol. 2001;146:923-39 pubmed
    RNA 3 of alfalfa mosaic virus (AMV) encodes the 5'-proximal movement protein (MP) gene and the 3'-proximal coat protein (CP) gene which is expressed from a subgenomic RNA...
  5. Guogas L, Filman D, Hogle J, Gehrke L. Cofolding organizes alfalfa mosaic virus RNA and coat protein for replication. Science. 2004;306:2108-11 pubmed publisher
    b>Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3' termini...
  6. Krab I, Caldwell C, Gallie D, Bol J. Coat protein enhances translational efficiency of Alfalfa mosaic virus RNAs and interacts with the eIF4G component of initiation factor eIF4F. J Gen Virol. 2005;86:1841-9 pubmed
    The three plus-strand genomic RNAs of Alfalfa mosaic virus (AMV) and the subgenomic messenger for viral coat protein (CP) contain a 5'-cap structure, but no 3'-poly(A) tail...
  7. Van Der Heijden M, Carette J, Reinhoud P, Haegi A, Bol J. Alfalfa mosaic virus replicase proteins P1 and P2 interact and colocalize at the vacuolar membrane. J Virol. 2001;75:1879-87 pubmed
    Replication of Alfalfa mosaic virus (AMV) RNAs depends on the virus-encoded proteins P1 and P2. P1 contains methyltransferase- and helicase-like domains, and P2 contains a polymerase-like domain...
  8. Brodzik R, Bandurska K, Deka D, Golovkin M, Koprowski H. Advances in alfalfa mosaic virus-mediated expression of anthrax antigen in planta. Biochem Biophys Res Commun. 2005;338:717-22 pubmed
    ..Here, we report the high yield and consistency in production of recombinant alfalfa mosaic virus (AlMV) particles for specific presentation of the small loop 15 amino acid epitope from domain-4 of the ..
  9. Chen S, Olsthoorn R. In vitro and in vivo studies of the RNA conformational switch in Alfalfa mosaic virus. J Virol. 2010;84:1423-9 pubmed publisher
    The 3' termini of Alfalfa mosaic virus (AMV) RNAs adopt two mutually exclusive conformations, a coat protein binding (CPB) and a tRNA-like (TL) conformer, which consist of a linear array of stem-loop structures and a pseudoknot structure, ..
  10. Vlot A, Neeleman L, Linthorst H, Bol J. Role of the 3'-untranslated regions of alfalfa mosaic virus RNAs in the formation of a transiently expressed replicase in plants and in the assembly of virions. J Virol. 2001;75:6440-9 pubmed
    b>Alfalfa mosaic virus (AMV) RNAs 1 and 2 encode the replicase proteins P1 and P2, respectively, whereas RNA 3 encodes the movement protein and the coat protein (CP)...

Detail Information

Publications78

  1. Venkatachalan S, Bushman J, Mercado J, Sancar F, CHRISTOPHERSON K, Boileau A. Optimized expression vector for ion channel studies in Xenopus oocytes and mammalian cells using alfalfa mosaic virus. Pflugers Arch. 2007;454:155-63 pubmed
    ..Furthermore, we found that expression using pUNIV was significantly enhanced in oocytes and was remarkably long-lasting in both systems...
  2. Neeleman L, Linthorst H, Bol J. Efficient translation of alfamovirus RNAs requires the binding of coat protein dimers to the 3' termini of the viral RNAs. J Gen Virol. 2004;85:231-40 pubmed
    The coat protein (CP) of Alfalfa mosaic virus (AMV) is required to initiate infection by the viral tripartite RNA genome whereas infection by the tripartite Brome mosaic virus (BMV) genome is independent of CP...
  3. Huang M, Jongejan L, Zheng H, Zhang L, Bol J. Intracellular localization and movement phenotypes of alfalfa mosaic virus movement protein mutants. Mol Plant Microbe Interact. 2001;14:1063-74 pubmed
    Thirteen mutations were introduced in the movement protein (MP) gene of Alfalfa mosaic virus (AMV) fused to the green fluorescent protein (GFP) gene and the mutant MP-GFP fusions were expressed transiently in tobacco protoplasts, tobacco ..
  4. Sanchez Navarro J, Miglino R, Ragozzino A, Bol J. Engineering of alfalfa mosaic virus RNA 3 into an expression vector. Arch Virol. 2001;146:923-39 pubmed
    RNA 3 of alfalfa mosaic virus (AMV) encodes the 5'-proximal movement protein (MP) gene and the 3'-proximal coat protein (CP) gene which is expressed from a subgenomic RNA...
  5. Guogas L, Filman D, Hogle J, Gehrke L. Cofolding organizes alfalfa mosaic virus RNA and coat protein for replication. Science. 2004;306:2108-11 pubmed publisher
    b>Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3' termini...
  6. Krab I, Caldwell C, Gallie D, Bol J. Coat protein enhances translational efficiency of Alfalfa mosaic virus RNAs and interacts with the eIF4G component of initiation factor eIF4F. J Gen Virol. 2005;86:1841-9 pubmed
    The three plus-strand genomic RNAs of Alfalfa mosaic virus (AMV) and the subgenomic messenger for viral coat protein (CP) contain a 5'-cap structure, but no 3'-poly(A) tail...
  7. Van Der Heijden M, Carette J, Reinhoud P, Haegi A, Bol J. Alfalfa mosaic virus replicase proteins P1 and P2 interact and colocalize at the vacuolar membrane. J Virol. 2001;75:1879-87 pubmed
    Replication of Alfalfa mosaic virus (AMV) RNAs depends on the virus-encoded proteins P1 and P2. P1 contains methyltransferase- and helicase-like domains, and P2 contains a polymerase-like domain...
  8. Brodzik R, Bandurska K, Deka D, Golovkin M, Koprowski H. Advances in alfalfa mosaic virus-mediated expression of anthrax antigen in planta. Biochem Biophys Res Commun. 2005;338:717-22 pubmed
    ..Here, we report the high yield and consistency in production of recombinant alfalfa mosaic virus (AlMV) particles for specific presentation of the small loop 15 amino acid epitope from domain-4 of the ..
  9. Chen S, Olsthoorn R. In vitro and in vivo studies of the RNA conformational switch in Alfalfa mosaic virus. J Virol. 2010;84:1423-9 pubmed publisher
    The 3' termini of Alfalfa mosaic virus (AMV) RNAs adopt two mutually exclusive conformations, a coat protein binding (CPB) and a tRNA-like (TL) conformer, which consist of a linear array of stem-loop structures and a pseudoknot structure, ..
  10. Vlot A, Neeleman L, Linthorst H, Bol J. Role of the 3'-untranslated regions of alfalfa mosaic virus RNAs in the formation of a transiently expressed replicase in plants and in the assembly of virions. J Virol. 2001;75:6440-9 pubmed
    b>Alfalfa mosaic virus (AMV) RNAs 1 and 2 encode the replicase proteins P1 and P2, respectively, whereas RNA 3 encodes the movement protein and the coat protein (CP)...
  11. Sánchez Navarro J, Bol J. Role of the alfalfa mosaic virus movement protein and coat protein in virus transport. Mol Plant Microbe Interact. 2001;14:1051-62 pubmed
    The movement protein (MP) and coat protein (CP) encoded by Alfalfa mosaic virus (AMV) RNA 3 are both required for virus transport...
  12. Aparicio F, Pallás V. The coat protein of Alfalfa mosaic virus interacts and interferes with the transcriptional activity of the bHLH transcription factor ILR3 promoting salicylic acid-dependent defence signalling response. Mol Plant Pathol. 2017;18:173-186 pubmed publisher
    ..b>Alfalfa mosaic virus (AMV) can establish a compatible interaction in tobacco and Arabidopsis hosts...
  13. Green B, Fujiki M, Mett V, Kaczmarczyk J, Shamloul M, Musiychuk K, et al. Transient protein expression in three Pisum sativum (green pea) varieties. Biotechnol J. 2009;4:230-7 pubmed publisher
    ..To address these limitations we screened a number of legumes for transient protein expression. Using the alfalfa mosaic virus (AMV) and the cucumber mosaic virus (CMV) vectors, delivered via Agrobacterium, we were able to identify ..
  14. Duijsings D, Kormelink R, Goldbach R. In vivo analysis of the TSWV cap-snatching mechanism: single base complementarity and primer length requirements. EMBO J. 2001;20:2545-52 pubmed
    ..for use during transcription initiation by tomato spotted wilt virus (TSWV) were tested using mutant alfalfa mosaic virus (AMV) RNAs as specific cap donors in transgenic Nicotiana tabacum plants expressing the AMV replicase ..
  15. Luque A, Reguera D. The structure of elongated viral capsids. Biophys J. 2010;98:2993-3003 pubmed publisher
    ..This work brings about a general classification of elongated viruses that will help to predict their structure, and to design viral cages with tailored geometrical properties for biomedical and nanotechnological applications. ..
  16. Bueso E, Serrano R, Pallas V, Sánchez Navarro J. Seed tolerance to deterioration in arabidopsis is affected by virus infection. Plant Physiol Biochem. 2017;116:1-8 pubmed publisher
    ..infection of Cauliflower mosaic virus (CaMV), Turnip mosaic virus (TuMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV) affects seed tolerance to deterioration measuring germination rates after an accelerated aging ..
  17. Ali A. Rapid detection of fifteen known soybean viruses by dot-immunobinding assay. J Virol Methods. 2017;249:126-129 pubmed publisher
    ..assay (DIBA) was optimized and used successfully for the rapid detection of 15 known viruses [Alfalfa mosaic virus (AMV), Bean pod mottle virus (BPMV), Bean yellow mosaic virus (BYMV), Cowpea mild mottle virus (CPMMV), ..
  18. Esfandiari N, Kohi Habibi M, Mosahebi G. Occurrence of viruses infecting pea in Iran. Commun Agric Appl Biol Sci. 2006;71:1281-7 pubmed
    A survey was conducted to determine the incidence of Alfalfa mosaic virus (AMV), Bean yellow mosaic virus (BYMV), Broad bean wilt virus-1 (BBWV), Pea leafroll virus (PLRV), Pea enation mosaic virus (PEMV), Pea seed borne mosaic virus (..
  19. Petrillo J, Rocheleau G, Kelley Clarke B, Gehrke L. Evaluation of the conformational switch model for alfalfa mosaic virus RNA replication. J Virol. 2005;79:5743-51 pubmed
    Key elements of the conformational switch model describing regulation of alfalfa mosaic virus (AMV) replication (R. C. Olsthoorn, S. Mertens, F. T. Brederode, and J. F. Bol, EMBO J...
  20. Wu B, Zwart M, Sánchez Navarro J, Elena S. Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus. Sci Rep. 2017;7:5004 pubmed publisher
    ..report experiments testing whether an evolutionary stable equilibrium exists for the three genomic RNAs of Alfalfa mosaic virus (AMV)...
  21. Martínez Pérez M, Aparicio F, López Gresa M, Bellés J, Sánchez Navarro J, Pallás V. Arabidopsis m6A demethylase activity modulates viral infection of a plant virus and the m6A abundance in its genomic RNAs. Proc Natl Acad Sci U S A. 2017;114:10755-10760 pubmed publisher
    ..identified the presence of m6A in the genomes of two members of the Bromoviridae family, alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV)...
  22. Stankovic I, Bulajić A, Vučurović A, Ristic D, Milojevic K, Berenji J, et al. Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. Acta Virol. 2011;55:337-47 pubmed
    ..wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively...
  23. Aparicio F, Sánchez Navarro J, Olsthoorn R, Pallas V, Bol J. Recognition of cis-acting sequences in RNA 3 of Prunus necrotic ringspot virus by the replicase of Alfalfa mosaic virus. J Gen Virol. 2001;82:947-51 pubmed
    b>Alfalfa mosaic virus (AMV) and Prunus necrotic ringspot virus (PNRSV) belong to the genera ALFAMOVIRUS: and ILARVIRUS:, respectively, of the family BROMOVIRIDAE: Initiation of infection by AMV and PNRSV requires binding of a few molecules ..
  24. Jayasena K, Randles J. A short insert in the leader sequence of RNA 3L, a long variant of Alfalfa mosaic virus RNA3, introduces two unidentified reading frames. Virus Genes. 2004;29:311-6 pubmed
    N20-RNA 3L, a large form of RNA 3 associated with Alfalfa mosaic virus (AMV) strain N20 comprises 2281 nt and has approximately 97% overall sequence similarity to the longest previously described RNA 3 of AMV strain YSMV (YSMV-RNA 3; 2188 ..
  25. Leastro M, Pallas V, Resende R, Sánchez Navarro J. The functional analysis of distinct tospovirus movement proteins (NSM) reveals different capabilities in tubule formation, cell-to-cell and systemic virus movement among the tospovirus species. Virus Res. 2017;227:57-68 pubmed publisher
    ..virus (TCSV) and Tomato spotted wilt virus (TSWV), which differ biologically and molecularly, by using the Alfalfa mosaic virus (AMV) model system...
  26. Scott S, Zimmerman M, Ge X. The sequence of RNA 1 and RNA 2 of tobacco streak virus: additional evidence for the inclusion of alfalfa mosaic virus in the genus Ilarvirus. Arch Virol. 1998;143:1187-98 pubmed
    ..Phylogenetic comparison of sequence data for RNA 1 with that of other ilarviruses and alfalfa mosaic virus (AMV) reveals two distinct clusters (TSV, CiLRV, and SpLV) and (AMV, PDV, and ApMV)...
  27. Ibrahim A, Hutchens H, Berg R, Loesch Fries L. Alfalfa mosaic virus replicase proteins, P1 and P2, localize to the tonoplast in the presence of virus RNA. Virology. 2012;433:449-61 pubmed publisher
    To identify the virus components important for assembly of the Alfalfa mosaic virus replicase complex, we used live cell imaging of Arabidopsis thaliana protoplasts that expressed various virus cDNAs encoding native and GFP-fusion ..
  28. Balasubramaniam M, Ibrahim A, Kim B, Loesch Fries L. Arabidopsis thaliana is an asymptomatic host of Alfalfa mosaic virus. Virus Res. 2006;121:215-9 pubmed
    The susceptibility of Arabidopsis thaliana ecotypes to infection by Alfalfa mosaic virus (AMV) was evaluated...
  29. Thole V, Garcia M, van Rossum CM -, Neeleman L, Brederode F, Linthorst H, et al. RNAs 1 and 2 of Alfalfa mosaic virus, expressed in transgenic plants, start to replicate only after infection of the plants with RNA 3. J Gen Virol. 2001;82:25-8 pubmed
    RNAs 1 and 2 of the tripartite genome of Alfalfa mosaic virus (AMV) encode the two viral replicase subunits. Full-length DNA copies of RNAs 1 and 2 were used to transform tobacco plants (R12 lines)...
  30. Mel nychuk M, Spyrydonov V. [Analysis of the viral capsid gene of Alfalfa mosaic virus (CP A1MV) cloned to the vector for plant transformation pBI121]. Mikrobiol Z. 2001;63:62-8 pubmed
  31. Guogas L, Laforest S, Gehrke L. Coat protein activation of alfalfa mosaic virus replication is concentration dependent. J Virol. 2005;79:5752-61 pubmed
    b>Alfalfa mosaic virus (AMV) and ilarvirus RNAs are infectious only in the presence of the viral coat protein; therefore, an understanding of coat protein's function is important for defining viral replication mechanisms...
  32. Panter S, Chu P, Ludlow E, Garrett R, Kalla R, Jahufer M, et al. Molecular breeding of transgenic white clover (Trifolium repens L.) with field resistance to Alfalfa mosaic virus through the expression of its coat protein gene. Transgenic Res. 2012;21:619-32 pubmed publisher
    Viral diseases, such as Alfalfa mosaic virus (AMV), cause significant reductions in the productivity and vegetative persistence of white clover plants in the field...
  33. Sánchez Navarro J, Carmen Herranz M, Pallas V. Cell-to-cell movement of Alfalfa mosaic virus can be mediated by the movement proteins of Ilar-, bromo-, cucumo-, tobamo- and comoviruses and does not require virion formation. Virology. 2006;346:66-73 pubmed
    RNA 3 of Alfalfa mosaic virus (AMV) encodes the movement protein (MP) and coat protein (CP)...
  34. Ansel McKinney P, Scott S, Swanson M, Ge X, Gehrke L. A plant viral coat protein RNA binding consensus sequence contains a crucial arginine. EMBO J. 1996;15:5077-84 pubmed
    A defining feature of alfalfa mosaic virus (AMV) and ilarviruses [type virus: tobacco streak virus (TSV)] is that, in addition to genomic RNAs, viral coat protein is required to establish infection in plants...
  35. Neeleman L, Olsthoorn R, Linthorst H, Bol J. Translation of a nonpolyadenylated viral RNA is enhanced by binding of viral coat protein or polyadenylation of the RNA. Proc Natl Acad Sci U S A. 2001;98:14286-91 pubmed
    ..Initiation of infection with the tripartite RNA genomes of alfalfa mosaic virus (AMV) and viruses from the genus Ilarvirus requires binding of a few molecules of coat protein (CP) to the ..
  36. Olsthoorn R, Bol J. Role of an essential triloop hairpin and flanking structures in the 3' untranslated region of Alfalfa mosaic virus RNA in in vitro transcription. J Virol. 2002;76:8747-56 pubmed
    The minus-strand promoter of Alfalfa mosaic virus (AMV), a tripartite plant virus belonging to the family Bromoviridae, is located within the 3'-terminal 145 nucleotides (nt), which can adopt a tRNA-like structure (TLS)...
  37. Vlot A, Bol J. The 5' untranslated region of alfalfa mosaic virus RNA 1 is involved in negative-strand RNA synthesis. J Virol. 2003;77:11284-9 pubmed
    The three genomic RNAs of alfalfa mosaic virus each contain a unique 5' untranslated region (5' UTR). Replacement of the 5' UTR of RNA 1 by that of RNA 2 or 3 yielded infectious replicons...
  38. van der Vossen E, Reusken C, Bol J. cis-preferential stimulation of alfalfa mosaic virus RNA 3 accumulation by the viral coat protein. Virology. 1996;220:163-70 pubmed
    RNA 3 of alfalfa mosaic virus (AIMV) encodes the movement protein P3 and the viral coat protein (CP) which is translated from the subgenomic RNA 4...
  39. Laforest S, Gehrke L. Spatial determinants of the alfalfa mosaic virus coat protein binding site. RNA. 2004;10:48-58 pubmed
    ..Here, we describe experiments that are aimed at understanding the functional significance of alfalfa mosaic virus RNA-coat protein binding, an interaction that parallels the initiation of viral RNA replication...
  40. Cihlar T, Fuller M, Cherrington J. Expression of the catalytic subunit (UL54) and the accessory protein (UL44) of human cytomegalovirus DNA polymerase in a coupled in vitro transcription/translation system. Protein Expr Purif. 1997;11:209-18 pubmed
    ..For expression of both UL54 and UL44, a truncated form of the alfalfa mosaic virus (AMV) RNA4 5'-UTR was found to be superior to the full-length AMV 5'-UTR or the original HCMV 5'-UTRs of ..
  41. Ansel McKinney P, Gehrke L. RNA determinants of a specific RNA-coat protein peptide interaction in alfalfa mosaic virus: conservation of homologous features in ilarvirus RNAs. J Mol Biol. 1998;278:767-85 pubmed
    b>Alfalfa mosaic virus (AMV) coat protein and tobacco streak virus (TSV) coat protein bind specifically to the 3' untranslated regions of the viral RNAs and are required with the genomic RNAs to initiate virus replication...
  42. Houwing C, Huis In t Veld M, Zuidema D, de Graaff M, Jaspars E. Natural minus-strand RNAs of alfalfa mosaic virus as in vitro templates for viral RNA polymerase. 3'-terminal non-coded guanosine and coat protein are insufficient factors for full-size plus-strand synthesis. Arch Virol. 2001;146:571-88 pubmed
    Replication complexes of alfalfa mosaic virus produce in vivo large quantities of plus-strand RNAs, but this production is fully dependent on the presence of coat protein...
  43. Ellinger T, Ehricht R, McCaskill J. In vitro evolution of molecular cooperation in CATCH, a cooperatively coupled amplification system. Chem Biol. 1998;5:729-41 pubmed
  44. Vlot A, Menard A, Bol J. Role of the alfalfa mosaic virus methyltransferase-like domain in negative-strand RNA synthesis. J Virol. 2002;76:11321-8 pubmed
    RNAs 1 and 2 of the tripartite genome of alfalfa mosaic virus (AMV) encode the replicase proteins P1 and P2, respectively...
  45. Gomase V, Kale K, Chikhale N, Changbhale S. Prediction of MHC binding peptides and epitopes from alfalfa mosaic virus. Curr Drug Discov Technol. 2007;4:117-215 pubmed
    Peptide fragments from alfalfa mosaic virus involved multiple antigenic components directing and empowering the immune system to protect the host from infection...
  46. Choi J, Kim B, Zhao X, Loesch Fries S. The importance of alfalfa mosaic virus coat protein dimers in the initiation of replication. Virology. 2003;305:44-9 pubmed
    Deletion and substitution mutations affecting the oligomerization of alfalfa mosaic virus (AMV) coat protein (CP) were studied in protoplasts to determine their effect on genome activation, an early step in AMV replication...
  47. Boyce M, Scott F, Guogas L, Gehrke L. Base-pairing potential identified by in vitro selection predicts the kinked RNA backbone observed in the crystal structure of the alfalfa mosaic virus RNA-coat protein complex. J Mol Recognit. 2006;19:68-78 pubmed
    The three-dimensional structure of the 3' terminus of alfalfa mosaic virus RNA in complex with an amino-terminal coat protein peptide revealed an unusual RNA fold with inter-AUGC basepairing stabilized by key arginine residues (Guogas, et ..
  48. Yusibov V, Mett V, Mett V, Davidson C, Musiychuk K, Gilliam S, et al. Peptide-based candidate vaccine against respiratory syncytial virus. Vaccine. 2005;23:2261-5 pubmed
    ..representing amino acids 170-190 of the respiratory syncytial virus (RSV) G protein as a fusion with the Alfalfa mosaic virus (AlMV) coat protein (CP), produced recombinant AlMV particles presenting this peptide (VMR-RSV) on their ..
  49. Vlot A, Laros S, Bol J. Coordinate replication of alfalfa mosaic virus RNAs 1 and 2 involves cis- and trans-acting functions of the encoded helicase-like and polymerase-like domains. J Virol. 2003;77:10790-8 pubmed
    RNAs 1 and 2 of the tripartite genome of alfalfa mosaic virus encode the replicase proteins P1 and P2, respectively, whereas RNA 3 encodes the movement protein and coat protein...
  50. Reichert V, Choi M, Petrillo J, Gehrke L. Alfalfa mosaic virus coat protein bridges RNA and RNA-dependent RNA polymerase in vitro. Virology. 2007;364:214-26 pubmed
    b>Alfalfa mosaic virus (AMV) RNA replication requires the viral coat protein (CP)...
  51. Swanson M, Ansel McKinney P, Houser Scott F, Yusibov V, Loesch Fries L, Gehrke L. Viral coat protein peptides with limited sequence homology bind similar domains of alfalfa mosaic virus and tobacco streak virus RNAs. J Virol. 1998;72:3227-34 pubmed
    An unusual and distinguishing feature of alfalfa mosaic virus (AMV) and ilarviruses such as tobacco streak virus (TSV) is that the viral coat protein is required to activate the early stages of viral RNA replication, a phenomenon known as ..
  52. Narayanan K, Han S. Genetic Modifications of Icosahedral Plant Virus-based Nanoparticles for Vaccine and Immunotherapy Applications. Curr Protein Pept Sci. 2017;18:1141-1151 pubmed publisher
    ..Here, we comprehensively review the developments of several recombinant PVNs as prophylactic and/or therapeutic vaccines for the prevention or treatment of several microbial diseases, pathologies, and toxin poisoning...
  53. Yusibov V, Hooper D, Spitsin S, Fleysh N, Kean R, Mikheeva T, et al. Expression in plants and immunogenicity of plant virus-based experimental rabies vaccine. Vaccine. 2002;20:3155-64 pubmed
    ..N protein) (amino acids 404-418) was PCR-amplified and cloned as a translational fusion product with the alfalfa mosaic virus (AlMV) coat protein (CP). This recombinant CP was expressed in two plant virus-based expression systems...
  54. Rampitsch C, Eastwell K. The complete nucleotide sequence of prune dwarf ilarvirus RNA-1. Arch Virol. 1997;142:1911-8 pubmed
    ..These findings are consistent with the inclusion of alfalfa mosaic virus in the ilarvirus genus...
  55. Tzareva N, Makhno V, Boni I. Ribosome-messenger recognition in the absence of the Shine-Dalgarno interactions. FEBS Lett. 1994;337:189-94 pubmed
    ..Protein S1 appears to be a key recognition element. S1 binds to sequences within the leaders of (-SD)mRNAs thus providing their affinity to E. coli ribosomes...
  56. Sánchez Navarro J, Zwart M, Elena S. Effects of the number of genome segments on primary and systemic infections with a multipartite plant RNA virus. J Virol. 2013;87:10805-15 pubmed publisher
    ..Here, Alfalfa mosaic virus (AMV), a tripartite Alfamovirus, and transgenic Nicotianatabacum plants expressing no (wild type), one (P2),..
  57. Jaspars E, Houwing C. A genome-activating N-terminal coat protein peptide of alfalfa mosaic virus is able to activate infection by RNAs 1, 2 and 3 but not by RNAs 1 and 2. Further support for the messenger release hypothesis. Arch Virol. 2002;147:857-63 pubmed
    An N-terminal genome-activating peptide of 25 amino acid residues of alfalfa mosaic virus coat protein was unable to activate the incomplete viral genome consisting of RNAs 1 and 2...
  58. Bleckley S, Stone J, Schroeder S. Crumple: a method for complete enumeration of all possible pseudoknot-free RNA secondary structures. PLoS ONE. 2012;7:e52414 pubmed publisher
    ..Pairing constraints reduce conformational space more than single nucleotide constraints. Examples with Alfalfa Mosaic Virus RNA and Trypanosome brucei guide RNA demonstrate the importance of evaluating all possible structures when ..
  59. Haasnoot P, Bol J, Olsthoorn R. A plant virus replication system to assay the formation of RNA pseudotriloop motifs in RNA-protein interactions. Proc Natl Acad Sci U S A. 2003;100:12596-600 pubmed
    ..Our data suggest that the pseudotriloop may represent a common motif in RNA-protein recognition...
  60. Rocheleau G, Petrillo J, Guogas L, Gehrke L. Degenerate in vitro genetic selection reveals mutations that diminish alfalfa mosaic virus RNA replication without affecting coat protein binding. J Virol. 2004;78:8036-46 pubmed
    The alfalfa mosaic virus (AMV) RNAs are infectious only in the presence of the viral coat protein; however, the mechanisms describing coat protein's role during replication are disputed...
  61. Harries P, Palanichelvam K, Bhat S, Nelson R. Tobacco mosaic virus 126-kDa protein increases the susceptibility of Nicotiana tabacum to other viruses and its dosage affects virus-induced gene silencing. Mol Plant Microbe Interact. 2008;21:1539-48 pubmed publisher
    ..tabacum increases susceptibility to a broad assortment of viruses, including Alfalfa mosaic virus, Brome mosaic virus, Tobacco rattle virus (TRV), and Potato virus X...
  62. Geerts Dimitriadou C, Zwart M, Goldbach R, Kormelink R. Base-pairing promotes leader selection to prime in vitro influenza genome transcription. Virology. 2011;409:17-26 pubmed publisher
    ..Altogether the results indicated an optimal cap donor consensus sequence of (7m)G-(N)(7-8)-(A/U/G)-(A/U)-AGC-3'...
  63. Dai Z, Hooker B, Quesenberry R, Thomas S. Optimization of Acidothermus cellulolyticus endoglucanase (E1) production in transgenic tobacco plants by transcriptional, post-transcription and post-translational modification. Transgenic Res. 2005;14:627-43 pubmed
    ..Mac promoter or light-inducible tomato Rubisco small sub-unit (RbcS-3C) promoter with its original or Alfalfa Mosaic Virus (AMV) RNA4 5'-untranslated leader (UTL) and targeted to different sub-cellular compartments via transit ..
  64. Larson S, Lucas R, McPherson A. Crystallographic structure of the T=1 particle of brome mosaic virus. J Mol Biol. 2005;346:815-31 pubmed
    ..T=1 particles was made with the reassembled T=1 particles produced from the coat protein of trypsin treated alfalfa mosaic virus (AlMV), another bromovirus. There is little resemblance between the two particles...
  65. Olsthoorn R, Haasnoot P, Bol J. Similarities and differences between the subgenomic and minus-strand promoters of an RNA plant virus. J Virol. 2004;78:4048-53 pubmed
    ..The minus-strand promoter of Alfalfa mosaic virus (AMV), a plant virus of the family Bromoviridae, consists of a triloop hairpin (hpE) which is attached to a ..
  66. Zhang X, Sato S, Ye X, Dorrance A, Morris T, Clemente T, et al. Robust RNAi-based resistance to mixed infection of three viruses in soybean plants expressing separate short hairpins from a single transgene. Phytopathology. 2011;101:1264-9 pubmed publisher
    ..Three such short IRs with sequences of unrelated soybean-infecting viruses (Alfalfa mosaic virus, Bean pod mottle virus, and Soybean mosaic virus) were assembled into a single transgene under control of ..
  67. Fajardo T, Peiro A, Pallas V, Sánchez Navarro J. Systemic transport of Alfalfa mosaic virus can be mediated by the movement proteins of several viruses assigned to five genera of the 30K family. J Gen Virol. 2013;94:677-81 pubmed publisher
    We previously showed that the movement protein (MP) gene of Alfalfa mosaic virus (AMV) is functionally exchangeable for the cell-to-cell transport of the corresponding genes of Tobacco mosaic virus (TMV), Brome mosaic virus, Prunus ..
  68. Olsthoorn R, Mertens S, Brederode F, Bol J. A conformational switch at the 3' end of a plant virus RNA regulates viral replication. EMBO J. 1999;18:4856-64 pubmed
    ..of the effect of magnesium, we demonstrate that this pseudoknot is required for replication of alfalfa mosaic virus. This conformation resembles the tRNA-like structure of the related bromo- and cucumo-viruses...
  69. Shiel P, Berger P. The complete nucleotide sequence of apple mosaic virus (ApMV) RNA 1 and RNA 2: ApMV is more closely related to alfalfa mosaic virus than to other ilarviruses. J Gen Virol. 2000;81:273-8 pubmed
    ..to all of the other ilarviruses for which sequence data are available, but both are more closely related to alfalfa mosaic virus (AMV) than to other ilarviruses...
  70. Kim B, Halk E, Merlo D, Nelson S, Loesch Fries L. Phosphorylation of alfalfa mosaic virus movement protein in vivo. Arch Virol. 2014;159:1787-91 pubmed publisher
    The 32-kDa movement protein, P3, of alfalfa mosaic virus (AMV) is essential for cell-to-cell spread of the virus in plants...
  71. Gulati A, Alapati K, Murthy A, Savithri H, Murthy M. Structural studies on tobacco streak virus coat protein: Insights into the pleomorphic nature of ilarviruses. J Struct Biol. 2016;193:95-105 pubmed publisher
    ..TSV CP was found to be structurally similar to that of Alfalfa mosaic virus (AMV) accounting for similar mechanism of genome activation in alfamo and ilar viruses...
  72. Sánchez Navarro J, Reusken C, Bol J, Pallas V. Replication of alfalfa mosaic virus RNA 3 with movement and coat protein genes replaced by corresponding genes of Prunus necrotic ringspot ilarvirus. J Gen Virol. 1997;78 ( Pt 12):3171-6 pubmed
    b>Alfalfa mosaic virus (AMV) and Prunus necrotic ringspot virus (PNRSV) are tripartite positive-strand RNA plant viruses that encode functionally similar translation products...
  73. Esfandiari N, Kohi Habibi M, Mosahebi G, Mozafari J. Detection of Alfalfa mosaic virus (AMV) in pea field in Iran. Commun Agric Appl Biol Sci. 2005;70:407-10 pubmed
    ..Antibody Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA) technique using polyclonal antiserum of Alfalfa mosaic virus (AMV), AS-0001, DSMZ, Braunschweig, Germany). The samples were extracted in 0...
  74. Cañizares M, Nicholson L, Lomonossoff G. Use of viral vectors for vaccine production in plants. Immunol Cell Biol. 2005;83:263-70 pubmed
    ..In this review, we discuss advances made in this field...
  75. Sánchez Navarro J, Fajardo T, Zicca S, Pallas V, Stavolone L. Caulimoviridae tubule-guided transport is dictated by movement protein properties. J Virol. 2010;84:4109-12 pubmed publisher
    ..other, we tested the exchangeability of MPs encoded by DNA and RNA virus genomes by means of an engineered alfalfa mosaic virus (AMV) system...
  76. Parrella G, Acanfora N, Orílio A, Navas Castillo J. Complete nucleotide sequence of a Spanish isolate of alfalfa mosaic virus: evidence for additional genetic variability. Arch Virol. 2011;156:1049-52 pubmed publisher
    b>Alfalfa mosaic virus (AMV) is a plant virus that is distributed worldwide and can induce necrosis and/or yellow mosaic on a large variety of plant species, including commercially important crops...
  77. Geerts Dimitriadou C, Goldbach R, Kormelink R. Preferential use of RNA leader sequences during influenza A transcription initiation in vivo. Virology. 2011;409:27-32 pubmed publisher
    ..Here, these results were verified during an influenza infection in MDCK cells. Alfalfa mosaic virus RNA3 leader sequences mutated in their base complementarity to the viral template, or the nucleotides 5' of ..