Selective suppression of endothelial cell apoptosis by the high molecular weight form of adiponectinHideki Kobayashi
Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, Japan
Circ Res 94:e27-31. 2004
..The percentage of high molecular weight form (HMW) per total adiponectin was significantly lower in patients with coronary artery disease than ..
- The high molecular weight urinary matrix metalloproteinase (MMP) activity is a complex of gelatinase B/MMP-9 and neutrophil gelatinase-associated lipocalin (NGAL). Modulation of MMP-9 activity by NGAL
L Yan
Department of Surgery, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 276:37258-65. 2001
..Among these activities, the presence of a group of high molecular weight (HMW) MMPs independently serves as a multivariate predictor of the metastatic phenotype ()...
Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modelingP Schuck
Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 78:1606-19. 2000
....
- Adiponectin multimeric complexes and the metabolic syndrome trait cluster
Cristina Lara-Castro
Department of Nutrition Sciences, 1675 Webb Nutrition Sciences Bldg, Room 244, University of Alabama, Birmingham, AL 35294, USA
Diabetes 55:249-59. 2006
Adiponectin circulates in human plasma mainly as a 180-kDa low molecular weight (LMW) hexamer and a high molecular weight (HMW) multimer of approximately 360 kDa...
- Low molecular weight chitosan nanoparticulate system at low N:P ratio for nontoxic polynucleotide delivery
Mohamad Alameh
Institute of Biomedical Engineering, Department of Chemical Engineering, Ecole Polytechnique, Montreal, QC, Canada
Int J Nanomedicine 7:1399-414. 2012
..the optimal parameters of chitosan for synthetic small interfering RNA delivery were inconclusive with high molecular weight at high amine-to-phosphate (N:P) ratios apparently required for efficient transfection...
- Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin
Hironori Waki
Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, 113 8655, Japan
J Biol Chem 278:40352-63. 2003
..adiponectin expressed in NIH-3T3 or Escherichia coli formed a wide range of multimers from trimers to high molecular weight (HMW) multimers...
- TDP43 is a human low molecular weight neurofilament (hNFL) mRNA-binding protein
Michael J Strong
Cell Biology Research Group, Robarts Research Institute, London, Ontario, Canada
Mol Cell Neurosci 35:320-7. 2007
..We observed that TDP43 stabilizes the human low molecular weight (hNFL) mRNA through a direct interaction with the 3'UTR...
- The yeast nuclear pore complex: composition, architecture, and transport mechanism
M P Rout
The Rockefeller University, New York, NY 10021, USA
J Cell Biol 148:635-51. 2000
..Using these data, we present a map of the molecular architecture of the yeast NPC and provide evidence for a Brownian affinity gating mechanism for nucleocytoplasmic transport...
- Identification of a low-molecular weight TrkB antagonist with anxiolytic and antidepressant activity in mice
Maxime Cazorla
Neurobiology and Molecular Pharmacology, Centre de psychiatrie et Neurosciences, UMR 894 INSERM Université Paris Descartes, Paris, France
J Clin Invest 121:1846-57. 2011
..silico screening and high-capacity functional assays in recombinant and neuronal cells, we identified a low-molecular weight TrkB ligand (ANA-12) that prevented activation of the receptor by BDNF with a high potency...
- Effects of molecular weight and hydrolysis conditions on anticancer activity of fucoidans from sporophyll of Undaria pinnatifida
Chen Yang
Department of Marine Food Science and Technology, Kangnung National University, Gangneung, Gangwon 210 702, Republic of Korea
Int J Biol Macromol 43:433-7. 2008
Hydrolyzed fucoidans, from sporophyll of Undaria pinnatifida, were used to determine the effects of molecular weight (Mw) and hydrolysis conditions on cancer cell growth. Native fucoidans showed anticancer activity of 37.6%...
- The high and low molecular weight forms of hyaluronan have distinct effects on CD44 clustering
Cuixia Yang
Department of Molecular Biology Laboratory, Shanghai Jiaotong University School of Medicine, Shanghai 200233, China
J Biol Chem 287:43094-107. 2012
CD44 is a major cell surface receptor for the glycosaminoglycan hyaluronan (HA). Native high molecular weight hyaluronan (nHA) and oligosaccharides of hyaluronan (oHA) provoke distinct biological effects upon binding to CD44...
- Proteomic analysis of post-translational modifications
Matthias Mann
Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, DK 5230 Denmark
Nat Biotechnol 21:255-61. 2003
..Finally, stable isotope labeling strategies in combination with mass spectrometry have been applied successfully to study the dynamics of modifications...
- Improved low molecular weight Myc-Max inhibitors
Huabo Wang
Section of Hematology Oncology, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
Mol Cancer Ther 6:2399-408. 2007
..They further show that the compounds specifically target c-Myc, which exists in a dynamic and relatively unstructured state with only partial and transient alpha-helical content...
- Tumor-specific urinary matrix metalloproteinase fingerprinting: identification of high molecular weight urinary matrix metalloproteinase species
Roopali Roy
Department of Surgery, Children s Hospital, Boston, MA 02115, USA
Clin Cancer Res 14:6610-7. 2008
..The objective of the current study was to identify these high molecular weight (HMW) species, determine their potential as predictors of disease status, and ask whether a tumor-specific ..
- Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis
Oliver Wirths
Department of Molecular Psychiatry and Alzheimer, Graduate School, University Medicine Goettingen, 37075 Goettingen, Germany
J Biol Chem 285:41517-24. 2010
..These data indicate that 9D5 is a therapeutically and diagnostically effective monoclonal antibody targeting low molecular weight AβpE3 oligomers.
- High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation
Marc Lavertu
Department of Chemical Engineering, Institute of Biomedical Engineering, Ecole Polytechnique of Montreal, P O Box 6079, Station Centre Ville, Montreal, Que, Canada H3C 3A7
Biomaterials 27:4815-24. 2006
..is a biodegradable natural polysaccharide that has shown potential for gene delivery, although the ideal molecular weight (MW) and degree of deacetylation (DDA) for this application have not been elucidated...
- HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells
Peter Cherepanov
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B 3000, Leuven, Belgium
J Biol Chem 278:372-81. 2003
....
- Intact extracellular matrix and the maintenance of immune tolerance: high molecular weight hyaluronan promotes persistence of induced CD4+CD25+ regulatory T cells
Paul L Bollyky
Benaroya Research Institute, Seattle, WA 98101, USA
J Leukoc Biol 86:567-72. 2009
....
- Regulation of lung injury and repair by Toll-like receptors and hyaluronan
Dianhua Jiang
Department of Medicine, Section of Pulmonary and Critical Care Medicine, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520, USA
Nat Med 11:1173-9. 2005
..Hyaluronan-TLR2 and hyaluronan-TLR4 interactions provide signals that initiate inflammatory responses, maintain epithelial cell integrity and promote recovery from acute lung injury...
- Structures of low molecular weight inhibitors bound to MDMX and MDM2 reveal new approaches for p53-MDMX/MDM2 antagonist drug discovery
Grzegorz M Popowicz
Max Planck Institute of Biochemistry, Martinsried, Germany
Cell Cycle 9:1104-11. 2010
..Our study includes the first structure of the complex between MDMX and a small molecule and should aid in developing efficient scaffolds for binding to MDMX and/or MDM2...
- Role of receptor for hyaluronic acid-mediated motility (RHAMM) in low molecular weight hyaluronan (LMWHA)-mediated fibrosarcoma cell adhesion
Katerina Kouvidi
Department of Histology Embryology, University of Crete, Heraklion 71003, Greece
J Biol Chem 286:38509-20. 2011
..Low molecular weight HA (LMWHA) significantly increased (p ≤ 0...
- The regulatory particle of the Saccharomyces cerevisiae proteasome
M H Glickman
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell Biol 18:3149-62. 1998
..Overall, regulatory particles from yeasts and mammals are remarkably similar, suggesting that the specific mechanistic features of the proteasome have been closely conserved over the course of evolution...
- Effect of molecular weight and substitution on tissue uptake of hydroxyethyl starch: a meta-analysis of clinical studies
Romuald Bellmann
Department of Internal Medicine I, Medical University of Innsbruck, Innsbruck, Austria
Clin Pharmacokinet 51:225-36. 2012
..HES solutions of lower molecular weight and substitution have been developed to increase urinary excretion and reduce plasma persistence...
- Size-selectivity of the glomerular barrier to high molecular weight proteins: upper size limitations of shunt pathways
J Tencer
Department of Nephrology, University Hospital of Lund, Sweden
Kidney Int 53:709-15. 1998
..We postulate that very large macromolecules like IgM will not pass the glomerular filter at all under normal conditions, whereas the urine concentration of alpha2-macroglobulin will normally be extremely low...
- Small molecular weight protein-protein interaction antagonists: an insurmountable challenge?
Alexander Dömling
Department of Pharmaceutical Sciences and Chemistry, University of Pittsburgh, PA, USA
Curr Opin Chem Biol 12:281-91. 2008
Several years ago small molecular weight protein-protein interaction (PPI) antagonists were considered as the Mount Everest in drug discovery and generally regarded as too difficult to be targeted...
- Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica
Jing Wang
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
Int J Biol Macromol 46:6-12. 2010
A low molecular weight fucoidan (DFPS), obtained from the brown seaweed Laminaria japonica, was separated into three fractions by anion-exchange column chromatography...
- Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution
Tanyarut Boontheekul
Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
Biomaterials 26:2455-65. 2005
..study, we characterized gels formed using a combination of partial oxidation of polymer chains and a bimodal molecular weight distribution of polymer...
- Low molecular weight catalytic metalloporphyrin antioxidant AEOL 10150 protects lungs from fractionated radiation
Zahid N Rabbani
Department of Radiation Oncology, Durham Regional Hospital Duke University Medical Center, Durham, NC 27710, USA
Free Radic Res 41:1273-82. 2007
..Administration of AEOL10150 at 5 mg/kg BID during and after RT results in a significant protective effect from long-term RT-induced lung injury. Low dose (2.5 mg/kg BID) delivery of AEOL10150 has no beneficial radioprotective effects...
- Polymer vesicles in vivo: correlations with PEG molecular weight
Peter J Photos
Department of Chemical and Biomolecular Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104 6393, USA
J Control Release 90:323-34. 2003
..The dependence of tau(1/2) on PEG molecular weight is nonetheless limited by uptake into the liver and spleen-as with liposomes...
- High-molecular weight adiponectin is independently associated with the extent of coronary artery disease in men
Maximilian von Eynatten
Department of Nephrology, Technical University Munich, Ismaningerstr 22, 81675 Munich, Germany
Atherosclerosis 199:123-8. 2008
..Yet, there is considerable evidence that the high-molecular weight (HMW) complex of adiponectin is the major active form of this adipokine...
- Hyaluronan fragments act as an endogenous danger signal by engaging TLR2
Kara A Scheibner
Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, 1830 East Monument Street, Baltimore, MD 21205, USA
J Immunol 177:1272-81. 2006
..Furthermore, we show that intact high m.w. HA can inhibit TLR-2 signaling. Finally, we demonstrate that LMW HA can act as an adjuvant promoting Ag-specific T cell responses in vivo in wild-type but not TLR-2(null) mice...
- Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine
Klaus Kunath
Department of Pharmaceutics and Biopharmacy, Philipps University of Marburg, Ketzerbach 63, D 35032, Marburg Lahn, Germany
J Control Release 89:113-25. 2003
..The weight average molecular weight (M(w)) of synthesized LMW-PEI was determined as 5.4+/-0...
- Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction
K Mandai
Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, Kobe 651 22, Japan
J Cell Biol 139:517-28. 1997
..These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ...
- Rapid evolution and complex structural organization in genomic regions harboring multiple prolamin genes in the polyploid wheat genome
Shuangcheng Gao
Key Laboratory of Crop Germplasm and Biotechnology, MOA, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Zhongguancun, Beijing, PR China
Plant Mol Biol 65:189-203. 2007
....
- Bax is present as a high molecular weight oligomer/complex in the mitochondrial membrane of apoptotic cells
B Antonsson
Serono Pharmaceutical Research Institute, Serono International S A, 14 Chemin des Aulx, CH 1228 Plan les Ouates, Geneva, Switzerland
J Biol Chem 276:11615-23. 2001
..apoptosis inducer staurosporine or UV irradiation, Bax associated with mitochondria is present as two large molecular weight oligomers/complexes of 96,000 and 260,000 Da, which are integrated into the mitochondrial membrane...
- The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine
Xianfeng Zhou
College of Life Science, Jilin University, Changchun 130012, PR China
Biomaterials 29:111-7. 2008
Nonviral gene delivery systems based on conventional high molecular weight chitosans are efficient as DNA vaccine delivery system, but have poor physical properties such as aggregated shapes, low solubility at neutral pH, high viscosity ..
- Latent high molecular weight complex of transforming growth factor beta 1. Purification from human platelets and structural characterization
K Miyazono
Ludwig Institute for Cancer Research, Uppsala, Sweden
J Biol Chem 263:6407-15. 1988
..Incubations of the latent form of TGF-beta 1 at extreme pH values, in 0.02% SDS or in 8 M urea, lead to activation of TGF-beta 1, whereas the complex was resistant to treatment with 5 M NaCl or heat (3 min at 95 degrees C)...
- Intracellular trafficking and decondensation kinetics of chitosan-pDNA polyplexes
Marc Thibault
Institute of Biomedical Engineering, Department of Chemical Engineering, Ecole Polytechnique, Montreal, Quebec, Canada
Mol Ther 18:1787-95. 2010
..DNA (pDNA) polyplexes can be critically modulated by the polymer degree of deacetylation (DDA) and molecular weight (MW)...
- Testosterone selectively reduces the high molecular weight form of adiponectin by inhibiting its secretion from adipocytes
Aimin Xu
Department of Medicine and Research Center of Heart, Brain, Hormone, and Healthy Aging, University of Hong Kong, Hong Kong, China
J Biol Chem 280:18073-80. 2005
..Gel filtration analysis revealed that circulating adiponectin existed as the forms of high molecular weight (HMW), middle molecular weight, and low molecular weight complexes in both human and mice...
- Individual variability of salivary gland proteins in three Phlebotomus species
Iva Rohousova
Department of Parasitology, Faculty of Science, Charles University, Vinicna 7, 128 43 Prague 2, Czech Republic
Acta Trop 122:80-6. 2012
..In P. papatasi, several groups of protein profiles were observed with no relationship to the geographical origin. The biological role of salivary proteins variability is discussed...
- High molecular weight tropomyosins regulate osteoclast cytoskeletal morphology
Preeyal Kotadiya
Department of Physiology and Cell Biology, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA
Bone 43:951-60. 2008
..identified in mammals, they can broadly be classified into two groups based on protein size, that is, high molecular weight and low molecular weight isoforms...
- Low molecular weight chitosan inhibits obesity induced by feeding a high-fat diet long-term in mice
Maho Sumiyoshi
Division of Functional Histology, Department of Integrated Basic Medical Science, School of Medicine, Ehime University, Shitsukawa, Toon City, Ehime 791 0295, Japan
J Pharm Pharmacol 58:201-7. 2006
Three low molecular weight chitosans (molecular weight: 21, 46 and 130 kDa) obtained by enzymatic hydrolysis of a high molecular weight chitosan (average molecular weight: 650 kDa) had low viscosity and were water-soluble...
- Expression of trypsin-like serine peptidases in pre-imaginal stages of Aedes aegypti (Diptera: Culicidae)
Camila Mesquita-Rodrigues
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
Arch Insect Biochem Physiol 76:223-35. 2011
..Comparative analysis between the proteolytic enzymes expressed by larvae and pupae showed that substantial changes in the expression of active trypsin-like serine proteases occur during the developmental cycle of A. aegypti...
- Depolymerization of fucosylated chondroitin sulfate from sea cucumber, Pearsonothuria graeffei, via 60Co irradiation
Nian Wu
College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China
Carbohydr Polym 93:604-14. 2013
..The anticoagulant activities of the low molecular weight fragments were additionally evaluated...
- In vivo tumor targeting of tumor necrosis factor-alpha-loaded stealth nanoparticles: effect of MePEG molecular weight and particle size
Chao Fang
Department of Pharmacology, College of Basic Medical Sciences, Shanghai Jiao Tong University, Shanghai 200025, PR China
Eur J Pharm Sci 27:27-36. 2006
The aim of this study is to reveal the influence of methoxypolyethyleneglycol (MePEG) molecular weight and particle size of stealth nanoparticles on their in vivo tumor targeting properties...
- Synthesis, structure and properties of poly(L-lactide-co-ε-caprolactone) statistical copolymers
Jorge Fernandez
University of the Basque Country UPV EHU, Department of Mining Metallurgy Engineering and Materials Science, School of Engineering, Alameda de Urquijo s n 48013 Bilbao, Spain
J Mech Behav Biomed Mater 9:100-12. 2012
..Gel permeation chromatography (GPC) and (1)H NMR measurements were performed to determine the molecular weight, composition and chain microstructure of copolymers of L-lactide(LA):ε-caprolactone(CL) synthesized using 90:..
- Estimated glomerular filtration rate is a poor predictor of the concentration of middle molecular weight uremic solutes in chronic kidney disease
Nathalie Neirynck
Nephrology Section, Department of Internal Medicine, Ghent University Hospital, Gent, Belgium
PLoS ONE 7:e44201. 2012
..Since also middle molecular weight proteins have been associated with mortality and cardiovascular damage in Chronic Kidney Disease (CKD), we ..
- The HaeIV restriction modification system of Haemophilus aegyptius is encoded by a single polypeptide
A Piekarowicz
Institute of Microbiology, University of Warsaw, Nowy Swiat 67, Warsaw, 00 046, Poland
J Mol Biol 293:1055-65. 1999
..The MTase activity modified symmetrically located adenine residues on both strands within the recognition sequence yielding N6-methyl adenine. Furthermore, the MTase was active as a dimer...
- The impact of treatment density and molecular weight for fractional laser-assisted drug delivery
Christina S Haak
Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, Denmark
J Control Release 163:335-41. 2012
..of this study was to investigate the impact of laser treatment density (% of skin occupied by channels) and molecular weight (MW) for fractional CO(2) laser-assisted drug delivery...
- Recruitment of glycosyl hydrolase proteins in a cone snail venomous arsenal: further insights into biomolecular features of Conus venoms
Aude Violette
Atheris Laboratories, Bernex Geneva, Switzerland
Mar Drugs 10:258-80. 2012
..We report here for the first time, the presence of high molecular weight compounds that participate in the envenomation cocktail used by these marine snails...
- Low molecular weight alkyl-polycation wrapped magnetite nanoparticle clusters as MRI probes for stem cell labeling and in vivo imaging
Gang Liu
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China
Biomaterials 32:528-37. 2011
..Different from many other systems involving high molecular polycations, we chose low molecular weight amphiphilic PEI2k to form stable nanocomplexes with SPIO nanoparticles...
- A proteomic view on the developmental transfer of homologous 30 kDa lipoproteins from peripheral fat body to perivisceral fat body via hemolymph in silkworm, Bombyx mori
Britto Cathrin Pakkianathan
Integrated Functional Genomics, Interdisciplinary Center for Clinical Research, University of Munster, Röntgenstr, 21, 48149 Munster, Germany
BMC Biochem 13:5. 2012
..Mass spectrometry (MS) was employed to elucidate individual protein sequences. Furthermore, 30 kDa proteins were purified and biochemically characterized...
- Mutant p53 targeting by the low molecular weight compound STIMA-1
Nicole Zache
Karolinska Institutet, Department of Oncology Pathology, Cancer Center Karolinska CCK, Karolinska University Hospital, Stockholm, Sweden
Mol Oncol 2:70-80. 2008
..Several small molecules that reactivate mutant p53 have been identified. Here we show that STIMA-1, a low molecular weight compound with some structural similarities to the previously identified molecule CP-31398, can stimulate ..
- Method for simultaneous imaging of endogenous low molecular weight metabolites in mouse brain using TiO2 nanoparticles in nanoparticle-assisted laser desorption/ionization-imaging mass spectrometry
Kamlesh Shrivas
Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1 20 1 Handayama, Higashi ku, Hamamatsu, Shizuoka 431 3192, Japan
Anal Chem 83:7283-9. 2011
We report the detection of a group of endogenous low molecular weight metabolites (LMWM) in mouse brain (80-500 Da) using TiO(2) nanoparticles (NPs) in nanoparticle-assisted laser desorption/ionization-imaging mass spectrometry (Nano-..
- Invertase from a strain of Rhodotorula glutinis
Maria C Rubio
Department of Industrial Microbiology, Institute of Biotechnology, Faculty of Biochemistry, Chemistry and Pharmacy, University of Tucuman, Ayacucho 491 4000, San Miguel de Tucuman, Argentina
Phytochemistry 61:605-9. 2002
..Invertase molecular weight was estimated to be 100 kDa by analytical gel filtration and 47 kDa by sodium dodecyl sulfate-polyacrylamide ..
- In vitro fermentation and prebiotic potential of novel low molecular weight polysaccharides derived from agar and alginate seaweeds
Priya Ramnani
Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK
Anaerobe 18:1-6. 2012
Fermentation properties and prebiotic potential of novel low molecular weight polysaccharides (LMWPs) derived from agar and alginate bearing seaweeds was investigated...
- A Mr 46,000 human milk fat globule protein that is highly expressed in human breast tumors contains factor VIII-like domains
D Larocca
John Muir Cancer and Aging Research Institute, Walnut Creek, California 94596
Cancer Res 51:4994-8. 1991
..one of the major components of the human milk fat globule, which identify a glycoprotein with an apparent molecular weight of 46,000, have been found to be useful for both breast cancer diagnosis and therapy...
- Inhibition of type I procollagen production in photodamage: correlation between presence of high molecular weight collagen fragments and reduced procollagen synthesis
James Varani
Department of Pathology, The University of Michigan Medical School, 1301 Catherine Road Box 0602, Ann Arbor, MI 48109, U S A
J Invest Dermatol 119:122-9. 2002
..These observations suggest that in the presence of high molecular weight fragments of type I collagen, type I procollagen synthesis is inhibited...
- Preparation of different molecular weight polysaccharides from Porphyridium cruentum and their antioxidant activities
Liqin Sun
Marine Biochemical Engineering Lab, Dalian University of Technology, Dalian, Liaoning, PR China
Int J Biol Macromol 45:42-7. 2009
..2, 60.66 and 6.55kDa. The antioxidant properties of different molecular weight polysaccharides were evaluated by determining the scavenging ability of free radicals, inhibitory effects on ..
- Chemoenzymatic synthesis of homogeneous ultralow molecular weight heparins
Yongmei Xu
Division of Medicinal Chemistry and Natural Products, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA
Science 334:498-501. 2011
Ultralow molecular weight (ULMW) heparins are sulfated glycans that are clinically used to treat thrombotic disorders. ULMW heparins range from 1500 to 3000 daltons, corresponding from 5 to 10 saccharide units...
- Lipoprotein-associated phospholipase A(2) mass and activity and risk of cardiovascular disease in a population with high prevalences of obesity and diabetes: the Strong Heart Study
Jorge R Kizer
Department of Medicine, Weill Cornell Medical Center, New York, New York, USA
Diabetes Care 35:840-7. 2012
....
- Albumin-bound low molecular weight thiols analysis in plasma and carotid plaques by CE
Angelo Zinellu
Dept Biomedical Sciences, University of Sassari, Sassari, Italy
J Sep Sci 33:126-31. 2010
We describe a new method for the quantification of low molecular weight thiols, as homocysteine, cysteine, cysteinylglycine, glutamylcysteine and glutathione bound to human plasma albumin...
- Characterisation of aquatic humic and non-humic matter with size-exclusion chromatography--organic carbon detection--organic nitrogen detection (LC-OCD-OND)
Stefan A Huber
DOC LABOR Dr Huber, Karlsruhe, Germany
Water Res 45:879-85. 2011
..with recent improvements in fraction assignation (humic substances, biopolymers, building blocks, low molecular weight organic acids and neutrals, hydrophobic organic carbon), the coupling of a novel organic nitrogen detector (..
- Non-esterified fatty acids generate distinct low-molecular weight amyloid-β (Aβ42) oligomers along pathway different from fibril formation
Amit Kumar
Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi, United States of America
PLoS ONE 6:e18759. 2011
..Among various aggregates, low-molecular weight soluble oligomers of Aβ are increasingly believed to be the primary neurotoxic agents responsible for memory ..
- Protein thermal shifts to identify low molecular weight fragments
James K Kranz
Biopharmaceutical Technologies, GlaxoSmithKline Biopharmaceutical Research and Development, Upper Merion, Pennsylvania, USA
Methods Enzymol 493:277-98. 2011
Measuring the strength of binding of low molecular weight ligands to a target protein is a significant challenge to fragment-based drug discovery that must be solved...
- Plasma total and high molecular weight adiponectin levels and risk of coronary heart disease in women
Tobias Pischon
Molecular Epidemiology Group, Max Delbrueck Center for Molecular Medicine Berlin Buch, Robert Roessle Strasse 10, 13125 Berlin, Germany
Atherosclerosis 219:322-9. 2011
To examine prospectively the association of total and high molecular weight (HMW) adiponectin, and HMW/total adiponectin ratio with risk of incident coronary heart disease (CHD) in women, and to examine to what extent adjustment for ..
- Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential
Luaine Bandounas
B Basic, Julianalaan 67, 2628 BC Delft, The Netherlands
BMC Biotechnol 11:94. 2011
..In this study, soil bacteria were isolated by enrichment on Kraft lignin and evaluated for their ligninolytic potential as a source of novel enzymes for waste lignin valorization...
- Paralogous proteins comprising the 150 kDa hydrophobic-ligand-binding-protein complex of the Taenia solium metacestode have evolved non-overlapped binding affinities toward fatty acid analogs
Seon Hee Kim
Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Center For Molecular Medicine, Samsung Biomedical Research Center, 300 Cheoncheon dong, Jangan gu, Suwon, Gyeonggi do 440 746, Republic of Korea
Int J Parasitol 41:1207-15. 2011
..These collective data suggest that the paralogous protein family have gained diverse functions during their evolution, to ensure the maintenance of metabolic homeostasis and survival of TsMs in hostile host environments...
- Effect of copolymer composition on the physicochemical characteristics, in vitro stability, and biodistribution of PLGA-mPEG nanoparticles
K Avgoustakis
Pharmaceutical Technology Laboratory, Department of Pharmacy, University of Patras, Rion 26500, Patras, Greece
Int J Pharm 259:115-27. 2003
..This may be associated with the effects of the mPEG/PLGA ratio on the density of PEG on the surface of the nanoparticles and on the size of the nanoparticles...
- Dynamic contrast-enhanced MR imaging kinetic parameters and molecular weight of dendritic contrast agents in tumor angiogenesis in mice
Quido G de Lussanet
Department of Radiology and Angiogenesis Laboratory, Departments of Pathology and Internal Medicine, Maastricht University Hospital, P Debyelaan 25, 6202 AZ Maastricht, The Netherlands
Radiology 235:65-72. 2005
....
- An essential cytoskeletal linker protein connecting actin microfilaments to intermediate filaments
Y Yang
Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA
Cell 86:655-65. 1996
..In BPAG1 null mice, axonal architecture is markedly perturbed, consistent with a failure to tether neurofilaments to the actin cytoskeleton and underscoring the physiological relevance of this protein...
- Phylogenetic analysis of C, M, N, and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci
Shunli Wang
Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, 100048 Beijing, China
Genome 54:273-84. 2011
..In addition, phylogenetic relationships among different genomes analysed in this study support the concept that Aegilops is not monophyletic...
- Wheat germ agglutinin. Molecular characteristics and specificity for sugar binding
Y Nagata
J Biol Chem 249:3116-22. 1974
- Purification and molecular characterization of exo-beta-1,3-glucanases from the marine yeast Williopsis saturnus WC91-2
Ying Peng
Key Laboratory of Marine Genetics and Gene Resource Exploitation of Ministry of Education, Ocean University of China, Yushan Road, No 5, Qingdao, China
Appl Microbiol Biotechnol 85:85-94. 2009
..Both matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectroscopy and DNA sequencing identified a peptide YIEAQLDAFEKR which is the conserved motif of the beta-1,3-glucanases from other yeasts...
- Bioreversibly crosslinked polyplexes of PEI and high molecular weight PEG show extended circulation times in vivo
Michael Neu
Department of Pharmaceutics and Biopharmacy, Philipps Universitat, 35037 Marburg, Germany
J Control Release 124:69-80. 2007
Copolymers consisting of branched PEI 25 kDa grafted with high molecular weight PEG at a low degree of substitution were successfully synthesized using a simple two-step procedure...
- Galactosylated low molecular weight chitosan as DNA carrier for hepatocyte-targeting
Shuying Gao
State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, Nanjing 210093, PR China
Int J Pharm 255:57-68. 2003
..Highly purified low molecular weight chitosan (LMWC) was prepared...
- Discovery of embelin as a cell-permeable, small-molecular weight inhibitor of XIAP through structure-based computational screening of a traditional herbal medicine three-dimensional structure database
Zaneta Nikolovska-Coleska
University of Michigan Comprehensive Cancer Center, Departments of Internal Medicine and Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0934, USA
J Med Chem 47:2430-40. 2004
..testing of selected candidate compounds, we discovered embelin from the Japanese Ardisia herb as a small-molecular weight inhibitor that binds to the XIAP BIR3 domain...
- Effect of charge and molecular weight on the functionality of gelatin carriers for corneal endothelial cell therapy
Jui Yang Lai
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013, ROC
Biomacromolecules 7:1836-44. 2006
..The carrier disks, consisting of gelatins with a different isoelectric point (IEP = 5.0 and 9.0) and a molecular weight (MW) ranging from 3 to 100 kDa, were subjected to 16.6 kGy gamma irradiation for sterilization...
- Structural characterization and anti-angiogenic properties of prostate-specific antigen isoforms in seminal fluid
Johanna M Mattsson
Department of Clinical Chemistry, Biomedicum Helsinki, Helsinki University Central Hospital and University of Helsinki, Helsinki, Finland
Prostate 68:945-54. 2008
..We have previously shown that seminal fluid contains both active and inactive, internally cleaved forms of PSA. The precise structural differences between these isoforms and their function are not known...
- Cross-correlated relaxation enhanced 1H[bond]13C NMR spectroscopy of methyl groups in very high molecular weight proteins and protein complexes
Vitali Tugarinov
Protein Engineering Network Centers of Excellence and Department of Medical Genetics, The University of Toronto, Toronto, Ontario, Canada M5S 1A8
J Am Chem Soc 125:10420-8. 2003
..The high quality of HMQC spectra obtained in short measuring times strongly suggests that methyl groups will be useful probes of structure and dynamics in supramolecular complexes...
- Low molecular weight polyethylenimine cross-linked by 2-hydroxypropyl-gamma-cyclodextrin coupled to peptide targeting HER2 as a gene delivery vector
Hongliang Huang
Institute of Immunology, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, PR China
Biomaterials 31:1830-8. 2010
..low cytotoxicity and high gene transfection efficiency, we synthesized a cationic polymer composed of low molecular weight polyethylenimine (PEI) of molecular weight of 600 Da cross-linked by 2-hydroxypropyl-gamma-cyclodextrin (HP ..
- Mannosylated chitosan-graft-polyethylenimine as a gene carrier for Raw 264.7 cell targeting
Hu Lin Jiang
Department of Agricultural Biotechnology, Seoul National University, Seoul 151 921, Republic of Korea
Int J Pharm 375:133-9. 2009
..The composition and molecular weight were characterized using (1)H NMR and GPC, respectively...
- Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex
T Guan
Department of Cell and Molecular Biology, Scripps Research Institute, La Jolla, California 92037, USA
Mol Biol Cell 6:1591-603. 1995
..The properties and localization of the p62 complex suggest that it may be involved in binding transport ligands near the center of the nuclear pore complex and in subsequently transferring them to the gated transport channel...
- Characterization of a Helicobacter pylori neutrophil-activating protein
D J Evans
Bacterial Enteropathogens Laboratory, Veterans Affairs Medical Center, Houston, Texas 77030, USA
Infect Immun 63:2213-20. 1995
..pylori isolates tested; however, there was considerable strain variation in the level of expression of HP-NAP activity in vitro. HP-NAP could play an important role in the gastric inflammatory response to H. pylori infection...
- Human intestinal maltase-glucoamylase: crystal structure of the N-terminal catalytic subunit and basis of inhibition and substrate specificity
Lyann Sim
Division of Cancer Genomics and Proteomics, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON, Canada
J Mol Biol 375:782-92. 2008
..The results provide a structural basis for the complementary roles of these glycosyl hydrolase family 31 subunits in the bioprocessing of complex starch structures into glucose...
- NMR analysis of a 900K GroEL GroES complex
Jocelyne Fiaux
Institut fur Molekularbiologie und Biophysik, Eidgenossische Technische Hochschule Zurich, CH 8093 Zurich, Switzerland
Nature 418:207-11. 2002
..This establishes the utility of these techniques for solution NMR studies that should permit the exploration of structure, dynamics and interactions in large macromolecular complexes...
- Small molecular weight proteins/peptides present in the in vivo formed human acquired enamel pellicle
Walter L Siqueira
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston University, 700 Albany Street, CABR, W201, Boston, MA 02118, United States
Arch Oral Biol 54:437-44. 2009
..The aim of this study was to investigate the type and the nature of peptides present in the in vivo formed human acquired enamel pellicle...
- A hydrophobic ligand-binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis
Eung Goo Lee
Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon, Korea
Proteomics 7:4016-30. 2007
..HLBP-mediated uptake of the host lipid may be critical for the parasite survival and thus could be targeted by chemotherapeutics and/or vaccine...
- Molecular-weight-dependent toxic effects of chitosans on the human keratinocyte cell line HaCaT
C Wiegand
Department of Dermatology, University Medical Center, Jena, Germany
Skin Pharmacol Physiol 23:164-70. 2010
..The influence of polycations like chitosan on human cells has been reported to depend on their molecular weight. However, the mechanism of cytotoxicity caused by polycations is not yet fully understood...
- Low molecular weight heat shock protein HSP27 is a prognostic indicator in rectal cancer but not colon cancer
Elizabeth M Tweedle
Royal Liverpool University Hospital, University of Liverpool, Liverpool, CH48 4DQ, UK
Gut 59:1501-10. 2010
..Finally, the relationship between HSP27 expression and outcome was examined in an independent cohort of 315 patients with a predominantly good prognosis...
- Low molecular weight protein-tyrosine phosphatase is involved in growth inhibition during cell differentiation
T Fiaschi
Dipartimento di Scienze Biochimiche, Universita' degli Studi di Firenze, 50134 Firenze, Italy
J Biol Chem 276:49156-63. 2001
Low molecular weight protein-tyrosine phosphatase (LMW-PTP) is an enzyme involved in mitogenic signaling and cytoskeletal rearrangement after platelet-derived growth factor (PDGF) stimulation...
- Characterization and comparative analysis of wheat high molecular weight glutenin subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS
Liyan Gao
Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, 100048 Beijing, China
J Agric Food Chem 58:2777-86. 2010
High molecular weight glutenin subunits (HMW-GS) from 60 germplasms including 30 common wheat cultivars and 30 related species were separated and characterized by a suite of separation methods including sodium dodecyl sulfate-..
- Frabin, a novel FGD1-related actin filament-binding protein capable of changing cell shape and activating c-Jun N-terminal kinase
H Obaishi
Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
J Biol Chem 273:18697-700. 1998
..Because FGD1 has been shown to serve as a GDP/GTP exchange protein for Cdc42 small G protein, it is likely that frabin is a direct linker between Cdc42 and the actin cytoskeleton...
- Design and chemical synthesis of a homogeneous polymer-modified erythropoiesis protein
Gerd G Kochendoerfer
Gryphon Therapeutics, 250 East Grand Avenue, Suite 90, South San Francisco, CA 94080, USA
Science 299:884-7. 2003
..These chemical methods are a powerful tool in the rational design of protein constructs with potential therapeutic applications...
- Estradiol loaded PLGA nanoparticles for oral administration: effect of polymer molecular weight and copolymer composition on release behavior in vitro and in vivo
G Mittal
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, S A S Nagar, Punjab, India
J Control Release 119:77-85. 2007
..formulations resulting in improved oral bioavailability and sustained release of estradiol by varying the molecular weight and copolymer composition of PLGA...
- Myosin light chain kinase in endothelium: molecular cloning and regulation
J G Garcia
Department of Medicine, Indiana University School of Medicine, and Richard Roudebush Veterans Administration Center, Indianapolis, USA
Am J Respir Cell Mol Biol 16:489-94. 1997
..Immunoprecipitation with NH2-specific antisera revealed a 214 kD high molecular weight MLCK in bovine and human endothelium which exhibits MLC phosphorylation properties...
- Simultaneous determination of protein aggregation, degradation, and absolute molecular weight by size exclusion chromatography-multiangle laser light scattering
Hongping Ye
Division of Pharmaceutical Analysis, Food and Drug Administration, 1114 Market Street, Room 1002, St Louis, MO 63101, USA
Anal Biochem 356:76-85. 2006
..Quantification and molecular weight determination were performed using commercial software...
- Controlled release of sirolimus from a multilayered PLGA stent matrix
Xintong Wang
School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
Biomaterials 27:5588-95. 2006
..The release of sirolimus can, on the other hand, be retarded by using a coating of a biodegradable polyester with a lauryl ester end group. Therefore, multilayered systems offer many options for controlling sirolimus release over months...
- Effect of chitosan structure properties and molecular weight on the intranasal absorption of tetramethylpyrazine phosphate in rats
Dan Mei
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
Eur J Pharm Biopharm 70:874-81. 2008
..The permeation enhancing effect of chitosan increased with increasing molecular weight up to M(w) 100 kDa...
- Systematic approach for validating the ubiquitinated proteome
Nicholas T Seyfried
Department of Human Genetics, Center for Neurodegenerative Diseases, Emory University, Atlanta, Georgia 30322, USA
Anal Chem 80:4161-9. 2008
..Because protein ubiquitination, especially polyubiquitination, causes a dramatic shift of molecular weight, the difference between experimental and expected molecular weight was used to confirm the status of ..
- The S-layer proteins of Lactobacillus crispatus strain ZJ001 is responsible for competitive exclusion against Escherichia coli O157:H7 and Salmonella typhimurium
Xueyan Chen
Zhejiang University Institute of Preventive Veterinary Medicine, and Zhejiang Provincial Key Laboratory of Prevent Veterinary Medicine, Hangzhou, PR China
Int J Food Microbiol 115:307-12. 2007
..typhimurium and E. coli O157:H7 to HeLa cells. These results suggest that L. crispatus ZJ001 possesses probiotic properties and the S-layer proteins are involved in the adhesion and competitive exclusion of pathogens to HeLa cells...
