molecular weight


Top Publications

  1. ncbi Selective suppression of endothelial cell apoptosis by the high molecular weight form of adiponectin
    Hideki Kobayashi
    Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, Japan
    Circ Res 94:e27-31. 2004
  2. ncbi The high molecular weight urinary matrix metalloproteinase (MMP) activity is a complex of gelatinase B/MMP-9 and neutrophil gelatinase-associated lipocalin (NGAL). Modulation of MMP-9 activity by NGAL
    L Yan
    Department of Surgery, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:37258-65. 2001
  3. pmc Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling
    P Schuck
    Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 78:1606-19. 2000
  4. ncbi Adiponectin multimeric complexes and the metabolic syndrome trait cluster
    Cristina Lara-Castro
    Department of Nutrition Sciences, 1675 Webb Nutrition Sciences Bldg, Room 244, University of Alabama, Birmingham, AL 35294, USA
    Diabetes 55:249-59. 2006
  5. pmc Low molecular weight chitosan nanoparticulate system at low N:P ratio for nontoxic polynucleotide delivery
    Mohamad Alameh
    Institute of Biomedical Engineering, Department of Chemical Engineering, Ecole Polytechnique, Montreal, QC, Canada
    Int J Nanomedicine 7:1399-414. 2012
  6. ncbi Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin
    Hironori Waki
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, 113 8655, Japan
    J Biol Chem 278:40352-63. 2003
  7. ncbi TDP43 is a human low molecular weight neurofilament (hNFL) mRNA-binding protein
    Michael J Strong
    Cell Biology Research Group, Robarts Research Institute, London, Ontario, Canada
    Mol Cell Neurosci 35:320-7. 2007
  8. pmc The yeast nuclear pore complex: composition, architecture, and transport mechanism
    M P Rout
    The Rockefeller University, New York, NY 10021, USA
    J Cell Biol 148:635-51. 2000
  9. pmc Identification of a low-molecular weight TrkB antagonist with anxiolytic and antidepressant activity in mice
    Maxime Cazorla
    Neurobiology and Molecular Pharmacology, Centre de psychiatrie et Neurosciences, UMR 894 INSERM Université Paris Descartes, Paris, France
    J Clin Invest 121:1846-57. 2011
  10. ncbi Effects of molecular weight and hydrolysis conditions on anticancer activity of fucoidans from sporophyll of Undaria pinnatifida
    Chen Yang
    Department of Marine Food Science and Technology, Kangnung National University, Gangneung, Gangwon 210 702, Republic of Korea
    Int J Biol Macromol 43:433-7. 2008

Detail Information

Publications290 found, 100 shown here

  1. ncbi Selective suppression of endothelial cell apoptosis by the high molecular weight form of adiponectin
    Hideki Kobayashi
    Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, Japan
    Circ Res 94:e27-31. 2004
    ..The percentage of high molecular weight form (HMW) per total adiponectin was significantly lower in patients with coronary artery disease than ..
  2. ncbi The high molecular weight urinary matrix metalloproteinase (MMP) activity is a complex of gelatinase B/MMP-9 and neutrophil gelatinase-associated lipocalin (NGAL). Modulation of MMP-9 activity by NGAL
    L Yan
    Department of Surgery, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:37258-65. 2001
    ..Among these activities, the presence of a group of high molecular weight (HMW) MMPs independently serves as a multivariate predictor of the metastatic phenotype ()...
  3. pmc Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling
    P Schuck
    Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 78:1606-19. 2000
  4. ncbi Adiponectin multimeric complexes and the metabolic syndrome trait cluster
    Cristina Lara-Castro
    Department of Nutrition Sciences, 1675 Webb Nutrition Sciences Bldg, Room 244, University of Alabama, Birmingham, AL 35294, USA
    Diabetes 55:249-59. 2006
    Adiponectin circulates in human plasma mainly as a 180-kDa low molecular weight (LMW) hexamer and a high molecular weight (HMW) multimer of approximately 360 kDa...
  5. pmc Low molecular weight chitosan nanoparticulate system at low N:P ratio for nontoxic polynucleotide delivery
    Mohamad Alameh
    Institute of Biomedical Engineering, Department of Chemical Engineering, Ecole Polytechnique, Montreal, QC, Canada
    Int J Nanomedicine 7:1399-414. 2012
    ..the optimal parameters of chitosan for synthetic small interfering RNA delivery were inconclusive with high molecular weight at high amine-to-phosphate (N:P) ratios apparently required for efficient transfection...
  6. ncbi Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin
    Hironori Waki
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, 113 8655, Japan
    J Biol Chem 278:40352-63. 2003
    ..adiponectin expressed in NIH-3T3 or Escherichia coli formed a wide range of multimers from trimers to high molecular weight (HMW) multimers...
  7. ncbi TDP43 is a human low molecular weight neurofilament (hNFL) mRNA-binding protein
    Michael J Strong
    Cell Biology Research Group, Robarts Research Institute, London, Ontario, Canada
    Mol Cell Neurosci 35:320-7. 2007
    ..We observed that TDP43 stabilizes the human low molecular weight (hNFL) mRNA through a direct interaction with the 3'UTR...
  8. pmc The yeast nuclear pore complex: composition, architecture, and transport mechanism
    M P Rout
    The Rockefeller University, New York, NY 10021, USA
    J Cell Biol 148:635-51. 2000
    ..Using these data, we present a map of the molecular architecture of the yeast NPC and provide evidence for a Brownian affinity gating mechanism for nucleocytoplasmic transport...
  9. pmc Identification of a low-molecular weight TrkB antagonist with anxiolytic and antidepressant activity in mice
    Maxime Cazorla
    Neurobiology and Molecular Pharmacology, Centre de psychiatrie et Neurosciences, UMR 894 INSERM Université Paris Descartes, Paris, France
    J Clin Invest 121:1846-57. 2011
    ..silico screening and high-capacity functional assays in recombinant and neuronal cells, we identified a low-molecular weight TrkB ligand (ANA-12) that prevented activation of the receptor by BDNF with a high potency...
  10. ncbi Effects of molecular weight and hydrolysis conditions on anticancer activity of fucoidans from sporophyll of Undaria pinnatifida
    Chen Yang
    Department of Marine Food Science and Technology, Kangnung National University, Gangneung, Gangwon 210 702, Republic of Korea
    Int J Biol Macromol 43:433-7. 2008
    Hydrolyzed fucoidans, from sporophyll of Undaria pinnatifida, were used to determine the effects of molecular weight (Mw) and hydrolysis conditions on cancer cell growth. Native fucoidans showed anticancer activity of 37.6%...
  11. pmc The high and low molecular weight forms of hyaluronan have distinct effects on CD44 clustering
    Cuixia Yang
    Department of Molecular Biology Laboratory, Shanghai Jiaotong University School of Medicine, Shanghai 200233, China
    J Biol Chem 287:43094-107. 2012
    CD44 is a major cell surface receptor for the glycosaminoglycan hyaluronan (HA). Native high molecular weight hyaluronan (nHA) and oligosaccharides of hyaluronan (oHA) provoke distinct biological effects upon binding to CD44...
  12. ncbi Proteomic analysis of post-translational modifications
    Matthias Mann
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, DK 5230 Denmark
    Nat Biotechnol 21:255-61. 2003
    ..Finally, stable isotope labeling strategies in combination with mass spectrometry have been applied successfully to study the dynamics of modifications...
  13. ncbi Improved low molecular weight Myc-Max inhibitors
    Huabo Wang
    Section of Hematology Oncology, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Mol Cancer Ther 6:2399-408. 2007
    ..They further show that the compounds specifically target c-Myc, which exists in a dynamic and relatively unstructured state with only partial and transient alpha-helical content...
  14. pmc Tumor-specific urinary matrix metalloproteinase fingerprinting: identification of high molecular weight urinary matrix metalloproteinase species
    Roopali Roy
    Department of Surgery, Children s Hospital, Boston, MA 02115, USA
    Clin Cancer Res 14:6610-7. 2008
    ..The objective of the current study was to identify these high molecular weight (HMW) species, determine their potential as predictors of disease status, and ask whether a tumor-specific ..
  15. pmc Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis
    Oliver Wirths
    Department of Molecular Psychiatry and Alzheimer, Graduate School, University Medicine Goettingen, 37075 Goettingen, Germany
    J Biol Chem 285:41517-24. 2010
    ..These data indicate that 9D5 is a therapeutically and diagnostically effective monoclonal antibody targeting low molecular weight AβpE3 oligomers.
  16. ncbi High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation
    Marc Lavertu
    Department of Chemical Engineering, Institute of Biomedical Engineering, Ecole Polytechnique of Montreal, P O Box 6079, Station Centre Ville, Montreal, Que, Canada H3C 3A7
    Biomaterials 27:4815-24. 2006 a biodegradable natural polysaccharide that has shown potential for gene delivery, although the ideal molecular weight (MW) and degree of deacetylation (DDA) for this application have not been elucidated...
  17. ncbi HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells
    Peter Cherepanov
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B 3000, Leuven, Belgium
    J Biol Chem 278:372-81. 2003
  18. pmc Intact extracellular matrix and the maintenance of immune tolerance: high molecular weight hyaluronan promotes persistence of induced CD4+CD25+ regulatory T cells
    Paul L Bollyky
    Benaroya Research Institute, Seattle, WA 98101, USA
    J Leukoc Biol 86:567-72. 2009
  19. ncbi Regulation of lung injury and repair by Toll-like receptors and hyaluronan
    Dianhua Jiang
    Department of Medicine, Section of Pulmonary and Critical Care Medicine, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520, USA
    Nat Med 11:1173-9. 2005
    ..Hyaluronan-TLR2 and hyaluronan-TLR4 interactions provide signals that initiate inflammatory responses, maintain epithelial cell integrity and promote recovery from acute lung injury...
  20. ncbi Structures of low molecular weight inhibitors bound to MDMX and MDM2 reveal new approaches for p53-MDMX/MDM2 antagonist drug discovery
    Grzegorz M Popowicz
    Max Planck Institute of Biochemistry, Martinsried, Germany
    Cell Cycle 9:1104-11. 2010
    ..Our study includes the first structure of the complex between MDMX and a small molecule and should aid in developing efficient scaffolds for binding to MDMX and/or MDM2...
  21. pmc Role of receptor for hyaluronic acid-mediated motility (RHAMM) in low molecular weight hyaluronan (LMWHA)-mediated fibrosarcoma cell adhesion
    Katerina Kouvidi
    Department of Histology Embryology, University of Crete, Heraklion 71003, Greece
    J Biol Chem 286:38509-20. 2011
    ..Low molecular weight HA (LMWHA) significantly increased (p ≤ 0...
  22. pmc The regulatory particle of the Saccharomyces cerevisiae proteasome
    M H Glickman
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 18:3149-62. 1998
    ..Overall, regulatory particles from yeasts and mammals are remarkably similar, suggesting that the specific mechanistic features of the proteasome have been closely conserved over the course of evolution...
  23. ncbi Effect of molecular weight and substitution on tissue uptake of hydroxyethyl starch: a meta-analysis of clinical studies
    Romuald Bellmann
    Department of Internal Medicine I, Medical University of Innsbruck, Innsbruck, Austria
    Clin Pharmacokinet 51:225-36. 2012
    ..HES solutions of lower molecular weight and substitution have been developed to increase urinary excretion and reduce plasma persistence...
  24. ncbi Size-selectivity of the glomerular barrier to high molecular weight proteins: upper size limitations of shunt pathways
    J Tencer
    Department of Nephrology, University Hospital of Lund, Sweden
    Kidney Int 53:709-15. 1998
    ..We postulate that very large macromolecules like IgM will not pass the glomerular filter at all under normal conditions, whereas the urine concentration of alpha2-macroglobulin will normally be extremely low...
  25. ncbi Small molecular weight protein-protein interaction antagonists: an insurmountable challenge?
    Alexander Dömling
    Department of Pharmaceutical Sciences and Chemistry, University of Pittsburgh, PA, USA
    Curr Opin Chem Biol 12:281-91. 2008
    Several years ago small molecular weight protein-protein interaction (PPI) antagonists were considered as the Mount Everest in drug discovery and generally regarded as too difficult to be targeted...
  26. ncbi Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica
    Jing Wang
    Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
    Int J Biol Macromol 46:6-12. 2010
    A low molecular weight fucoidan (DFPS), obtained from the brown seaweed Laminaria japonica, was separated into three fractions by anion-exchange column chromatography...
  27. ncbi Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution
    Tanyarut Boontheekul
    Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
    Biomaterials 26:2455-65. 2005, we characterized gels formed using a combination of partial oxidation of polymer chains and a bimodal molecular weight distribution of polymer...
  28. ncbi Low molecular weight catalytic metalloporphyrin antioxidant AEOL 10150 protects lungs from fractionated radiation
    Zahid N Rabbani
    Department of Radiation Oncology, Durham Regional Hospital Duke University Medical Center, Durham, NC 27710, USA
    Free Radic Res 41:1273-82. 2007
    ..Administration of AEOL10150 at 5 mg/kg BID during and after RT results in a significant protective effect from long-term RT-induced lung injury. Low dose (2.5 mg/kg BID) delivery of AEOL10150 has no beneficial radioprotective effects...
  29. ncbi Polymer vesicles in vivo: correlations with PEG molecular weight
    Peter J Photos
    Department of Chemical and Biomolecular Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104 6393, USA
    J Control Release 90:323-34. 2003
    ..The dependence of tau(1/2) on PEG molecular weight is nonetheless limited by uptake into the liver and spleen-as with liposomes...
  30. ncbi High-molecular weight adiponectin is independently associated with the extent of coronary artery disease in men
    Maximilian von Eynatten
    Department of Nephrology, Technical University Munich, Ismaningerstr 22, 81675 Munich, Germany
    Atherosclerosis 199:123-8. 2008
    ..Yet, there is considerable evidence that the high-molecular weight (HMW) complex of adiponectin is the major active form of this adipokine...
  31. ncbi Hyaluronan fragments act as an endogenous danger signal by engaging TLR2
    Kara A Scheibner
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, 1830 East Monument Street, Baltimore, MD 21205, USA
    J Immunol 177:1272-81. 2006
    ..Furthermore, we show that intact high m.w. HA can inhibit TLR-2 signaling. Finally, we demonstrate that LMW HA can act as an adjuvant promoting Ag-specific T cell responses in vivo in wild-type but not TLR-2(null) mice...
  32. ncbi Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine
    Klaus Kunath
    Department of Pharmaceutics and Biopharmacy, Philipps University of Marburg, Ketzerbach 63, D 35032, Marburg Lahn, Germany
    J Control Release 89:113-25. 2003
    ..The weight average molecular weight (M(w)) of synthesized LMW-PEI was determined as 5.4+/-0...
  33. pmc Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction
    K Mandai
    Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, Kobe 651 22, Japan
    J Cell Biol 139:517-28. 1997
    ..These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ...
  34. ncbi Rapid evolution and complex structural organization in genomic regions harboring multiple prolamin genes in the polyploid wheat genome
    Shuangcheng Gao
    Key Laboratory of Crop Germplasm and Biotechnology, MOA, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Zhongguancun, Beijing, PR China
    Plant Mol Biol 65:189-203. 2007
  35. ncbi Bax is present as a high molecular weight oligomer/complex in the mitochondrial membrane of apoptotic cells
    B Antonsson
    Serono Pharmaceutical Research Institute, Serono International S A, 14 Chemin des Aulx, CH 1228 Plan les Ouates, Geneva, Switzerland
    J Biol Chem 276:11615-23. 2001
    ..apoptosis inducer staurosporine or UV irradiation, Bax associated with mitochondria is present as two large molecular weight oligomers/complexes of 96,000 and 260,000 Da, which are integrated into the mitochondrial membrane...
  36. ncbi The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine
    Xianfeng Zhou
    College of Life Science, Jilin University, Changchun 130012, PR China
    Biomaterials 29:111-7. 2008
    Nonviral gene delivery systems based on conventional high molecular weight chitosans are efficient as DNA vaccine delivery system, but have poor physical properties such as aggregated shapes, low solubility at neutral pH, high viscosity ..
  37. ncbi Latent high molecular weight complex of transforming growth factor beta 1. Purification from human platelets and structural characterization
    K Miyazono
    Ludwig Institute for Cancer Research, Uppsala, Sweden
    J Biol Chem 263:6407-15. 1988
    ..Incubations of the latent form of TGF-beta 1 at extreme pH values, in 0.02% SDS or in 8 M urea, lead to activation of TGF-beta 1, whereas the complex was resistant to treatment with 5 M NaCl or heat (3 min at 95 degrees C)...
  38. pmc Intracellular trafficking and decondensation kinetics of chitosan-pDNA polyplexes
    Marc Thibault
    Institute of Biomedical Engineering, Department of Chemical Engineering, Ecole Polytechnique, Montreal, Quebec, Canada
    Mol Ther 18:1787-95. 2010
    ..DNA (pDNA) polyplexes can be critically modulated by the polymer degree of deacetylation (DDA) and molecular weight (MW)...
  39. ncbi Testosterone selectively reduces the high molecular weight form of adiponectin by inhibiting its secretion from adipocytes
    Aimin Xu
    Department of Medicine and Research Center of Heart, Brain, Hormone, and Healthy Aging, University of Hong Kong, Hong Kong, China
    J Biol Chem 280:18073-80. 2005
    ..Gel filtration analysis revealed that circulating adiponectin existed as the forms of high molecular weight (HMW), middle molecular weight, and low molecular weight complexes in both human and mice...
  40. ncbi Individual variability of salivary gland proteins in three Phlebotomus species
    Iva Rohousova
    Department of Parasitology, Faculty of Science, Charles University, Vinicna 7, 128 43 Prague 2, Czech Republic
    Acta Trop 122:80-6. 2012
    ..In P. papatasi, several groups of protein profiles were observed with no relationship to the geographical origin. The biological role of salivary proteins variability is discussed...
  41. pmc High molecular weight tropomyosins regulate osteoclast cytoskeletal morphology
    Preeyal Kotadiya
    Department of Physiology and Cell Biology, The Ohio State University, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA
    Bone 43:951-60. 2008
    ..identified in mammals, they can broadly be classified into two groups based on protein size, that is, high molecular weight and low molecular weight isoforms...
  42. ncbi Low molecular weight chitosan inhibits obesity induced by feeding a high-fat diet long-term in mice
    Maho Sumiyoshi
    Division of Functional Histology, Department of Integrated Basic Medical Science, School of Medicine, Ehime University, Shitsukawa, Toon City, Ehime 791 0295, Japan
    J Pharm Pharmacol 58:201-7. 2006
    Three low molecular weight chitosans (molecular weight: 21, 46 and 130 kDa) obtained by enzymatic hydrolysis of a high molecular weight chitosan (average molecular weight: 650 kDa) had low viscosity and were water-soluble...
  43. ncbi Expression of trypsin-like serine peptidases in pre-imaginal stages of Aedes aegypti (Diptera: Culicidae)
    Camila Mesquita-Rodrigues
    Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
    Arch Insect Biochem Physiol 76:223-35. 2011
    ..Comparative analysis between the proteolytic enzymes expressed by larvae and pupae showed that substantial changes in the expression of active trypsin-like serine proteases occur during the developmental cycle of A. aegypti...
  44. ncbi Depolymerization of fucosylated chondroitin sulfate from sea cucumber, Pearsonothuria graeffei, via 60Co irradiation
    Nian Wu
    College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China
    Carbohydr Polym 93:604-14. 2013
    ..The anticoagulant activities of the low molecular weight fragments were additionally evaluated...
  45. ncbi In vivo tumor targeting of tumor necrosis factor-alpha-loaded stealth nanoparticles: effect of MePEG molecular weight and particle size
    Chao Fang
    Department of Pharmacology, College of Basic Medical Sciences, Shanghai Jiao Tong University, Shanghai 200025, PR China
    Eur J Pharm Sci 27:27-36. 2006
    The aim of this study is to reveal the influence of methoxypolyethyleneglycol (MePEG) molecular weight and particle size of stealth nanoparticles on their in vivo tumor targeting properties...
  46. ncbi Synthesis, structure and properties of poly(L-lactide-co-ε-caprolactone) statistical copolymers
    Jorge Fernandez
    University of the Basque Country UPV EHU, Department of Mining Metallurgy Engineering and Materials Science, School of Engineering, Alameda de Urquijo s n 48013 Bilbao, Spain
    J Mech Behav Biomed Mater 9:100-12. 2012
    ..Gel permeation chromatography (GPC) and (1)H NMR measurements were performed to determine the molecular weight, composition and chain microstructure of copolymers of L-lactide(LA):ε-caprolactone(CL) synthesized using 90:..
  47. pmc Estimated glomerular filtration rate is a poor predictor of the concentration of middle molecular weight uremic solutes in chronic kidney disease
    Nathalie Neirynck
    Nephrology Section, Department of Internal Medicine, Ghent University Hospital, Gent, Belgium
    PLoS ONE 7:e44201. 2012
    ..Since also middle molecular weight proteins have been associated with mortality and cardiovascular damage in Chronic Kidney Disease (CKD), we ..
  48. ncbi The HaeIV restriction modification system of Haemophilus aegyptius is encoded by a single polypeptide
    A Piekarowicz
    Institute of Microbiology, University of Warsaw, Nowy Swiat 67, Warsaw, 00 046, Poland
    J Mol Biol 293:1055-65. 1999
    ..The MTase activity modified symmetrically located adenine residues on both strands within the recognition sequence yielding N6-methyl adenine. Furthermore, the MTase was active as a dimer...
  49. ncbi The impact of treatment density and molecular weight for fractional laser-assisted drug delivery
    Christina S Haak
    Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, Denmark
    J Control Release 163:335-41. 2012
    ..of this study was to investigate the impact of laser treatment density (% of skin occupied by channels) and molecular weight (MW) for fractional CO(2) laser-assisted drug delivery...
  50. pmc Recruitment of glycosyl hydrolase proteins in a cone snail venomous arsenal: further insights into biomolecular features of Conus venoms
    Aude Violette
    Atheris Laboratories, Bernex Geneva, Switzerland
    Mar Drugs 10:258-80. 2012
    ..We report here for the first time, the presence of high molecular weight compounds that participate in the envenomation cocktail used by these marine snails...
  51. ncbi Low molecular weight alkyl-polycation wrapped magnetite nanoparticle clusters as MRI probes for stem cell labeling and in vivo imaging
    Gang Liu
    National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China
    Biomaterials 32:528-37. 2011
    ..Different from many other systems involving high molecular polycations, we chose low molecular weight amphiphilic PEI2k to form stable nanocomplexes with SPIO nanoparticles...
  52. pmc A proteomic view on the developmental transfer of homologous 30 kDa lipoproteins from peripheral fat body to perivisceral fat body via hemolymph in silkworm, Bombyx mori
    Britto Cathrin Pakkianathan
    Integrated Functional Genomics, Interdisciplinary Center for Clinical Research, University of Munster, Röntgenstr, 21, 48149 Munster, Germany
    BMC Biochem 13:5. 2012
    ..Mass spectrometry (MS) was employed to elucidate individual protein sequences. Furthermore, 30 kDa proteins were purified and biochemically characterized...
  53. ncbi Mutant p53 targeting by the low molecular weight compound STIMA-1
    Nicole Zache
    Karolinska Institutet, Department of Oncology Pathology, Cancer Center Karolinska CCK, Karolinska University Hospital, Stockholm, Sweden
    Mol Oncol 2:70-80. 2008
    ..Several small molecules that reactivate mutant p53 have been identified. Here we show that STIMA-1, a low molecular weight compound with some structural similarities to the previously identified molecule CP-31398, can stimulate ..
  54. ncbi Method for simultaneous imaging of endogenous low molecular weight metabolites in mouse brain using TiO2 nanoparticles in nanoparticle-assisted laser desorption/ionization-imaging mass spectrometry
    Kamlesh Shrivas
    Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1 20 1 Handayama, Higashi ku, Hamamatsu, Shizuoka 431 3192, Japan
    Anal Chem 83:7283-9. 2011
    We report the detection of a group of endogenous low molecular weight metabolites (LMWM) in mouse brain (80-500 Da) using TiO(2) nanoparticles (NPs) in nanoparticle-assisted laser desorption/ionization-imaging mass spectrometry (Nano-..
  55. ncbi Invertase from a strain of Rhodotorula glutinis
    Maria C Rubio
    Department of Industrial Microbiology, Institute of Biotechnology, Faculty of Biochemistry, Chemistry and Pharmacy, University of Tucuman, Ayacucho 491 4000, San Miguel de Tucuman, Argentina
    Phytochemistry 61:605-9. 2002
    ..Invertase molecular weight was estimated to be 100 kDa by analytical gel filtration and 47 kDa by sodium dodecyl sulfate-polyacrylamide ..
  56. ncbi In vitro fermentation and prebiotic potential of novel low molecular weight polysaccharides derived from agar and alginate seaweeds
    Priya Ramnani
    Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK
    Anaerobe 18:1-6. 2012
    Fermentation properties and prebiotic potential of novel low molecular weight polysaccharides (LMWPs) derived from agar and alginate bearing seaweeds was investigated...
  57. ncbi A Mr 46,000 human milk fat globule protein that is highly expressed in human breast tumors contains factor VIII-like domains
    D Larocca
    John Muir Cancer and Aging Research Institute, Walnut Creek, California 94596
    Cancer Res 51:4994-8. 1991 of the major components of the human milk fat globule, which identify a glycoprotein with an apparent molecular weight of 46,000, have been found to be useful for both breast cancer diagnosis and therapy...
  58. ncbi Inhibition of type I procollagen production in photodamage: correlation between presence of high molecular weight collagen fragments and reduced procollagen synthesis
    James Varani
    Department of Pathology, The University of Michigan Medical School, 1301 Catherine Road Box 0602, Ann Arbor, MI 48109, U S A
    J Invest Dermatol 119:122-9. 2002
    ..These observations suggest that in the presence of high molecular weight fragments of type I collagen, type I procollagen synthesis is inhibited...
  59. ncbi Preparation of different molecular weight polysaccharides from Porphyridium cruentum and their antioxidant activities
    Liqin Sun
    Marine Biochemical Engineering Lab, Dalian University of Technology, Dalian, Liaoning, PR China
    Int J Biol Macromol 45:42-7. 2009
    ..2, 60.66 and 6.55kDa. The antioxidant properties of different molecular weight polysaccharides were evaluated by determining the scavenging ability of free radicals, inhibitory effects on ..
  60. pmc Chemoenzymatic synthesis of homogeneous ultralow molecular weight heparins
    Yongmei Xu
    Division of Medicinal Chemistry and Natural Products, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA
    Science 334:498-501. 2011
    Ultralow molecular weight (ULMW) heparins are sulfated glycans that are clinically used to treat thrombotic disorders. ULMW heparins range from 1500 to 3000 daltons, corresponding from 5 to 10 saccharide units...
  61. pmc Lipoprotein-associated phospholipase A(2) mass and activity and risk of cardiovascular disease in a population with high prevalences of obesity and diabetes: the Strong Heart Study
    Jorge R Kizer
    Department of Medicine, Weill Cornell Medical Center, New York, New York, USA
    Diabetes Care 35:840-7. 2012
  62. ncbi Albumin-bound low molecular weight thiols analysis in plasma and carotid plaques by CE
    Angelo Zinellu
    Dept Biomedical Sciences, University of Sassari, Sassari, Italy
    J Sep Sci 33:126-31. 2010
    We describe a new method for the quantification of low molecular weight thiols, as homocysteine, cysteine, cysteinylglycine, glutamylcysteine and glutathione bound to human plasma albumin...
  63. ncbi Characterisation of aquatic humic and non-humic matter with size-exclusion chromatography--organic carbon detection--organic nitrogen detection (LC-OCD-OND)
    Stefan A Huber
    DOC LABOR Dr Huber, Karlsruhe, Germany
    Water Res 45:879-85. 2011
    ..with recent improvements in fraction assignation (humic substances, biopolymers, building blocks, low molecular weight organic acids and neutrals, hydrophobic organic carbon), the coupling of a novel organic nitrogen detector (..
  64. pmc Non-esterified fatty acids generate distinct low-molecular weight amyloid-β (Aβ42) oligomers along pathway different from fibril formation
    Amit Kumar
    Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi, United States of America
    PLoS ONE 6:e18759. 2011
    ..Among various aggregates, low-molecular weight soluble oligomers of Aβ are increasingly believed to be the primary neurotoxic agents responsible for memory ..
  65. ncbi Protein thermal shifts to identify low molecular weight fragments
    James K Kranz
    Biopharmaceutical Technologies, GlaxoSmithKline Biopharmaceutical Research and Development, Upper Merion, Pennsylvania, USA
    Methods Enzymol 493:277-98. 2011
    Measuring the strength of binding of low molecular weight ligands to a target protein is a significant challenge to fragment-based drug discovery that must be solved...
  66. pmc Plasma total and high molecular weight adiponectin levels and risk of coronary heart disease in women
    Tobias Pischon
    Molecular Epidemiology Group, Max Delbrueck Center for Molecular Medicine Berlin Buch, Robert Roessle Strasse 10, 13125 Berlin, Germany
    Atherosclerosis 219:322-9. 2011
    To examine prospectively the association of total and high molecular weight (HMW) adiponectin, and HMW/total adiponectin ratio with risk of incident coronary heart disease (CHD) in women, and to examine to what extent adjustment for ..
  67. pmc Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential
    Luaine Bandounas
    B Basic, Julianalaan 67, 2628 BC Delft, The Netherlands
    BMC Biotechnol 11:94. 2011
    ..In this study, soil bacteria were isolated by enrichment on Kraft lignin and evaluated for their ligninolytic potential as a source of novel enzymes for waste lignin valorization...
  68. ncbi Paralogous proteins comprising the 150 kDa hydrophobic-ligand-binding-protein complex of the Taenia solium metacestode have evolved non-overlapped binding affinities toward fatty acid analogs
    Seon Hee Kim
    Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Center For Molecular Medicine, Samsung Biomedical Research Center, 300 Cheoncheon dong, Jangan gu, Suwon, Gyeonggi do 440 746, Republic of Korea
    Int J Parasitol 41:1207-15. 2011
    ..These collective data suggest that the paralogous protein family have gained diverse functions during their evolution, to ensure the maintenance of metabolic homeostasis and survival of TsMs in hostile host environments...
  69. ncbi Effect of copolymer composition on the physicochemical characteristics, in vitro stability, and biodistribution of PLGA-mPEG nanoparticles
    K Avgoustakis
    Pharmaceutical Technology Laboratory, Department of Pharmacy, University of Patras, Rion 26500, Patras, Greece
    Int J Pharm 259:115-27. 2003
    ..This may be associated with the effects of the mPEG/PLGA ratio on the density of PEG on the surface of the nanoparticles and on the size of the nanoparticles...
  70. ncbi Dynamic contrast-enhanced MR imaging kinetic parameters and molecular weight of dendritic contrast agents in tumor angiogenesis in mice
    Quido G de Lussanet
    Department of Radiology and Angiogenesis Laboratory, Departments of Pathology and Internal Medicine, Maastricht University Hospital, P Debyelaan 25, 6202 AZ Maastricht, The Netherlands
    Radiology 235:65-72. 2005
  71. ncbi An essential cytoskeletal linker protein connecting actin microfilaments to intermediate filaments
    Y Yang
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA
    Cell 86:655-65. 1996
    ..In BPAG1 null mice, axonal architecture is markedly perturbed, consistent with a failure to tether neurofilaments to the actin cytoskeleton and underscoring the physiological relevance of this protein...
  72. ncbi Phylogenetic analysis of C, M, N, and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci
    Shunli Wang
    Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, 100048 Beijing, China
    Genome 54:273-84. 2011
    ..In addition, phylogenetic relationships among different genomes analysed in this study support the concept that Aegilops is not monophyletic...
  73. ncbi Wheat germ agglutinin. Molecular characteristics and specificity for sugar binding
    Y Nagata
    J Biol Chem 249:3116-22. 1974
  74. ncbi Purification and molecular characterization of exo-beta-1,3-glucanases from the marine yeast Williopsis saturnus WC91-2
    Ying Peng
    Key Laboratory of Marine Genetics and Gene Resource Exploitation of Ministry of Education, Ocean University of China, Yushan Road, No 5, Qingdao, China
    Appl Microbiol Biotechnol 85:85-94. 2009
    ..Both matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectroscopy and DNA sequencing identified a peptide YIEAQLDAFEKR which is the conserved motif of the beta-1,3-glucanases from other yeasts...
  75. ncbi Bioreversibly crosslinked polyplexes of PEI and high molecular weight PEG show extended circulation times in vivo
    Michael Neu
    Department of Pharmaceutics and Biopharmacy, Philipps Universitat, 35037 Marburg, Germany
    J Control Release 124:69-80. 2007
    Copolymers consisting of branched PEI 25 kDa grafted with high molecular weight PEG at a low degree of substitution were successfully synthesized using a simple two-step procedure...
  76. ncbi Galactosylated low molecular weight chitosan as DNA carrier for hepatocyte-targeting
    Shuying Gao
    State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, Nanjing 210093, PR China
    Int J Pharm 255:57-68. 2003
    ..Highly purified low molecular weight chitosan (LMWC) was prepared...
  77. ncbi Discovery of embelin as a cell-permeable, small-molecular weight inhibitor of XIAP through structure-based computational screening of a traditional herbal medicine three-dimensional structure database
    Zaneta Nikolovska-Coleska
    University of Michigan Comprehensive Cancer Center, Departments of Internal Medicine and Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0934, USA
    J Med Chem 47:2430-40. 2004
    ..testing of selected candidate compounds, we discovered embelin from the Japanese Ardisia herb as a small-molecular weight inhibitor that binds to the XIAP BIR3 domain...
  78. ncbi Effect of charge and molecular weight on the functionality of gelatin carriers for corneal endothelial cell therapy
    Jui Yang Lai
    Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013, ROC
    Biomacromolecules 7:1836-44. 2006
    ..The carrier disks, consisting of gelatins with a different isoelectric point (IEP = 5.0 and 9.0) and a molecular weight (MW) ranging from 3 to 100 kDa, were subjected to 16.6 kGy gamma irradiation for sterilization...
  79. ncbi Structural characterization and anti-angiogenic properties of prostate-specific antigen isoforms in seminal fluid
    Johanna M Mattsson
    Department of Clinical Chemistry, Biomedicum Helsinki, Helsinki University Central Hospital and University of Helsinki, Helsinki, Finland
    Prostate 68:945-54. 2008
    ..We have previously shown that seminal fluid contains both active and inactive, internally cleaved forms of PSA. The precise structural differences between these isoforms and their function are not known...
  80. ncbi Cross-correlated relaxation enhanced 1H[bond]13C NMR spectroscopy of methyl groups in very high molecular weight proteins and protein complexes
    Vitali Tugarinov
    Protein Engineering Network Centers of Excellence and Department of Medical Genetics, The University of Toronto, Toronto, Ontario, Canada M5S 1A8
    J Am Chem Soc 125:10420-8. 2003
    ..The high quality of HMQC spectra obtained in short measuring times strongly suggests that methyl groups will be useful probes of structure and dynamics in supramolecular complexes...
  81. ncbi Low molecular weight polyethylenimine cross-linked by 2-hydroxypropyl-gamma-cyclodextrin coupled to peptide targeting HER2 as a gene delivery vector
    Hongliang Huang
    Institute of Immunology, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, PR China
    Biomaterials 31:1830-8. 2010
    ..low cytotoxicity and high gene transfection efficiency, we synthesized a cationic polymer composed of low molecular weight polyethylenimine (PEI) of molecular weight of 600 Da cross-linked by 2-hydroxypropyl-gamma-cyclodextrin (HP ..
  82. ncbi Mannosylated chitosan-graft-polyethylenimine as a gene carrier for Raw 264.7 cell targeting
    Hu Lin Jiang
    Department of Agricultural Biotechnology, Seoul National University, Seoul 151 921, Republic of Korea
    Int J Pharm 375:133-9. 2009
    ..The composition and molecular weight were characterized using (1)H NMR and GPC, respectively...
  83. pmc Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex
    T Guan
    Department of Cell and Molecular Biology, Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 6:1591-603. 1995
    ..The properties and localization of the p62 complex suggest that it may be involved in binding transport ligands near the center of the nuclear pore complex and in subsequently transferring them to the gated transport channel...
  84. pmc Characterization of a Helicobacter pylori neutrophil-activating protein
    D J Evans
    Bacterial Enteropathogens Laboratory, Veterans Affairs Medical Center, Houston, Texas 77030, USA
    Infect Immun 63:2213-20. 1995
    ..pylori isolates tested; however, there was considerable strain variation in the level of expression of HP-NAP activity in vitro. HP-NAP could play an important role in the gastric inflammatory response to H. pylori infection...
  85. ncbi Human intestinal maltase-glucoamylase: crystal structure of the N-terminal catalytic subunit and basis of inhibition and substrate specificity
    Lyann Sim
    Division of Cancer Genomics and Proteomics, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON, Canada
    J Mol Biol 375:782-92. 2008
    ..The results provide a structural basis for the complementary roles of these glycosyl hydrolase family 31 subunits in the bioprocessing of complex starch structures into glucose...
  86. ncbi NMR analysis of a 900K GroEL GroES complex
    Jocelyne Fiaux
    Institut fur Molekularbiologie und Biophysik, Eidgenossische Technische Hochschule Zurich, CH 8093 Zurich, Switzerland
    Nature 418:207-11. 2002
    ..This establishes the utility of these techniques for solution NMR studies that should permit the exploration of structure, dynamics and interactions in large macromolecular complexes...
  87. pmc Small molecular weight proteins/peptides present in the in vivo formed human acquired enamel pellicle
    Walter L Siqueira
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston University, 700 Albany Street, CABR, W201, Boston, MA 02118, United States
    Arch Oral Biol 54:437-44. 2009
    ..The aim of this study was to investigate the type and the nature of peptides present in the in vivo formed human acquired enamel pellicle...
  88. ncbi A hydrophobic ligand-binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis
    Eung Goo Lee
    Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon, Korea
    Proteomics 7:4016-30. 2007
    ..HLBP-mediated uptake of the host lipid may be critical for the parasite survival and thus could be targeted by chemotherapeutics and/or vaccine...
  89. ncbi Molecular-weight-dependent toxic effects of chitosans on the human keratinocyte cell line HaCaT
    C Wiegand
    Department of Dermatology, University Medical Center, Jena, Germany
    Skin Pharmacol Physiol 23:164-70. 2010
    ..The influence of polycations like chitosan on human cells has been reported to depend on their molecular weight. However, the mechanism of cytotoxicity caused by polycations is not yet fully understood...
  90. ncbi Low molecular weight heat shock protein HSP27 is a prognostic indicator in rectal cancer but not colon cancer
    Elizabeth M Tweedle
    Royal Liverpool University Hospital, University of Liverpool, Liverpool, CH48 4DQ, UK
    Gut 59:1501-10. 2010
    ..Finally, the relationship between HSP27 expression and outcome was examined in an independent cohort of 315 patients with a predominantly good prognosis...
  91. ncbi Low molecular weight protein-tyrosine phosphatase is involved in growth inhibition during cell differentiation
    T Fiaschi
    Dipartimento di Scienze Biochimiche, Universita' degli Studi di Firenze, 50134 Firenze, Italy
    J Biol Chem 276:49156-63. 2001
    Low molecular weight protein-tyrosine phosphatase (LMW-PTP) is an enzyme involved in mitogenic signaling and cytoskeletal rearrangement after platelet-derived growth factor (PDGF) stimulation...
  92. ncbi Characterization and comparative analysis of wheat high molecular weight glutenin subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS
    Liyan Gao
    Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, 100048 Beijing, China
    J Agric Food Chem 58:2777-86. 2010
    High molecular weight glutenin subunits (HMW-GS) from 60 germplasms including 30 common wheat cultivars and 30 related species were separated and characterized by a suite of separation methods including sodium dodecyl sulfate-..
  93. ncbi Frabin, a novel FGD1-related actin filament-binding protein capable of changing cell shape and activating c-Jun N-terminal kinase
    H Obaishi
    Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
    J Biol Chem 273:18697-700. 1998
    ..Because FGD1 has been shown to serve as a GDP/GTP exchange protein for Cdc42 small G protein, it is likely that frabin is a direct linker between Cdc42 and the actin cytoskeleton...
  94. ncbi Design and chemical synthesis of a homogeneous polymer-modified erythropoiesis protein
    Gerd G Kochendoerfer
    Gryphon Therapeutics, 250 East Grand Avenue, Suite 90, South San Francisco, CA 94080, USA
    Science 299:884-7. 2003
    ..These chemical methods are a powerful tool in the rational design of protein constructs with potential therapeutic applications...
  95. ncbi Estradiol loaded PLGA nanoparticles for oral administration: effect of polymer molecular weight and copolymer composition on release behavior in vitro and in vivo
    G Mittal
    Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, S A S Nagar, Punjab, India
    J Control Release 119:77-85. 2007
    ..formulations resulting in improved oral bioavailability and sustained release of estradiol by varying the molecular weight and copolymer composition of PLGA...
  96. ncbi Myosin light chain kinase in endothelium: molecular cloning and regulation
    J G Garcia
    Department of Medicine, Indiana University School of Medicine, and Richard Roudebush Veterans Administration Center, Indianapolis, USA
    Am J Respir Cell Mol Biol 16:489-94. 1997
    ..Immunoprecipitation with NH2-specific antisera revealed a 214 kD high molecular weight MLCK in bovine and human endothelium which exhibits MLC phosphorylation properties...
  97. ncbi Simultaneous determination of protein aggregation, degradation, and absolute molecular weight by size exclusion chromatography-multiangle laser light scattering
    Hongping Ye
    Division of Pharmaceutical Analysis, Food and Drug Administration, 1114 Market Street, Room 1002, St Louis, MO 63101, USA
    Anal Biochem 356:76-85. 2006
    ..Quantification and molecular weight determination were performed using commercial software...
  98. ncbi Controlled release of sirolimus from a multilayered PLGA stent matrix
    Xintong Wang
    School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
    Biomaterials 27:5588-95. 2006
    ..The release of sirolimus can, on the other hand, be retarded by using a coating of a biodegradable polyester with a lauryl ester end group. Therefore, multilayered systems offer many options for controlling sirolimus release over months...
  99. ncbi Effect of chitosan structure properties and molecular weight on the intranasal absorption of tetramethylpyrazine phosphate in rats
    Dan Mei
    School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
    Eur J Pharm Biopharm 70:874-81. 2008
    ..The permeation enhancing effect of chitosan increased with increasing molecular weight up to M(w) 100 kDa...
  100. pmc Systematic approach for validating the ubiquitinated proteome
    Nicholas T Seyfried
    Department of Human Genetics, Center for Neurodegenerative Diseases, Emory University, Atlanta, Georgia 30322, USA
    Anal Chem 80:4161-9. 2008
    ..Because protein ubiquitination, especially polyubiquitination, causes a dramatic shift of molecular weight, the difference between experimental and expected molecular weight was used to confirm the status of ..
  101. ncbi The S-layer proteins of Lactobacillus crispatus strain ZJ001 is responsible for competitive exclusion against Escherichia coli O157:H7 and Salmonella typhimurium
    Xueyan Chen
    Zhejiang University Institute of Preventive Veterinary Medicine, and Zhejiang Provincial Key Laboratory of Prevent Veterinary Medicine, Hangzhou, PR China
    Int J Food Microbiol 115:307-12. 2007
    ..typhimurium and E. coli O157:H7 to HeLa cells. These results suggest that L. crispatus ZJ001 possesses probiotic properties and the S-layer proteins are involved in the adhesion and competitive exclusion of pathogens to HeLa cells...

Research Grants71

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
    ..We also found that I/R increased activity of two highly abundant unknown MMPs with molecular weight range 100 - 150 kD in the cardiomyocyte nucleus...
  3. Targeting Galectin-3 with novel drugs for treatment of eczema
    Fu Tong Liu; Fiscal Year: 2010
    ..likely to have higher affinity for galectin-3 and more specific for galectin-3 over other galectins than low molecular weight compounds...
  4. Non-viral Genetic Modification of Antigen-presenting Cells in Allografts
    Wilson S Meng; Fiscal Year: 2011
    ..The system entails using the low molecular weight polycation O10H6 as a DNA condensing agent on the surface of nickel-displaying PLGA particles...
  5. Role of the Mammalian Siderophore in Cellular Iron Metabolism and Erythropoiesis
    Laxminarayana R Devireddy; Fiscal Year: 2012
    ..If successful, this grant could help explain the causes of iron deficiency anemia and could help in designing better drugs to combat iron deficiency anemia. ..
  6. Phage Display for Improved Peptide-based Tumor Targeting and Imaging Agents
    Susan L Deutscher; Fiscal Year: 2013
    ..However, high molecular weight antibodies as well as small peptides may not be ideal as cancer imaging agents in vivo...
  7. Tissue-specific delivery of probes by control of membrane trafficking of endoprot
    Blake Peterson; Fiscal Year: 2010
    ..This proposal is focused the creation of low molecular weight cholesterylamine-linked peptides (<3000 daltons) that selectively deliver fluorescent molecular probes ..
  8. Molecular Basis of E. coli Adhesins in Bladder Disorders
    Scott J Hultgren; Fiscal Year: 2013
    ..Aim 3 investigates the efficacy of potent small molecular weight compounds called mannosides, which block FimH function, to treat and prevent UTI in the context of these ..
  9. Therapeutic Effect of a Novel Antioxidant on Degenerative Eye Disorders
    Nuran Ercal; Fiscal Year: 2012
    ..Lens and retinal cells combat oxidative stress by generating sufficient antioxidant enzymes or small molecular weight antioxidants like glutathione (GSH)...
  10. Identification of Proteins Important for Male Osteoporosis
    Hong Wen Deng; Fiscal Year: 2013
    ..studies is the limited detection of low abundance proteins and proteins with extreme isoelectric point, molecular weight, and hydrophobicity, especially membrane proteins...
  11. Role of the kallikrein-kinin system in diabetic retinopathy
    Edward P Feener; Fiscal Year: 2012
    ..We demonstrated that plasma kallikrein, factor XII, and high molecular weight kininogen, are present in their activated forms in vitreous from people with diabetic retinopathy...
  12. Protection of Brain Injury from Cyanide Poisoning by Carnosic Acid
    Stuart A Lipton; Fiscal Year: 2013
    ..To investigate neuroprotective effects of CA in an in vitro model of cyanide poisoning. Specific Aim 2. To examine neuroprotective effects of CA in an in vivo mouse model mimicking the effects of cyanide ingestion. ..
  13. Combined BAC Transgenic and Knock-Out Mouse Model of Lowe Syndrome Nephropathy
    Robert L Nussbaum; Fiscal Year: 2013
    ..The renal tubular abnormalities of LOCR, particularly low molecular weight (LMW) proteinuria and aminoaciduria, overlap with those of Dent disease, a disorder that affects only the ..
  14. Intranasal CNS delivery of drugs against organophosphorous threat agents
    ..The major advantage of this approach is that charged molecules or even high molecular weight drugs which cannot bypass the BBB under physiological conditions can be successfully delivered to the brain...
  15. Mindfulness Based Stress Reduction as an Adjunctive Treatment for Psoriasis
    Jan A Moynihan; Fiscal Year: 2013
    ..Cytokines (small molecular weight messenger proteins) produced by T helper (Th)1 and Th17 lymphocytes, antigen presenting cells, and leukocytes ..
  16. Characterization of IL36, a novel cytokine
    Albert Zlotnik; Fiscal Year: 2012
    ..a signal peptide of 45 amino acids, predicting a mature protein of 266 amino acids, with an estimated molecular weight of ~29KDa...
  17. Development of Novel LANCL2-based Anti-diabetic Compounds
    JOSEP BASSAGANYA; Fiscal Year: 2013
    ..To optimize oral drug delivery we shall develop more analogs that have molecular properties (ClogP, molecular weight, polar surface area, numbers of rings, rotable bonds, N and O atoms, hydrogen bond donors and acceptors) ..
  18. Acquisition of a MALDI-TOF/TOF MS for Glycomic and Lipidomic Research
    Larry S Schlesinger; Fiscal Year: 2010
    ..The AB 4800MALDI will come equipped to acquire data in linear mode for high molecular weight analysis, reflectron mode for high resolution analysis and true precursor selected MS/MS in both positive and ..
  19. Novel Small Molecules For Acute Liver Failure
    Prakash Narayan; Fiscal Year: 2009
    ..Low molecular weight compounds that mimic the activity of SF/HGF could overcome these logistical difficulties and provide ..
  20. Mechanisms regulating SR Ca2+ ATPase in the Atria
    Muthu Periasamy; Fiscal Year: 2012
    ..The activity of SERCA pump is regulated by two small molecular weight proteins, phospholamban (PLB) and sarcolipin (SLN)...
  21. The Effect Of Deafness Associated Mutations on MyosinVIIA Function
    Eva Forgacs; Fiscal Year: 2010
    ..Analytical ultracentrifugation experiments will be conducted so that the molecular weight of the wild type and mutant myosin VIIA proteins can be accurately measured...
  22. Novel Glycosaminoglycan Ethers for Prevention of Metastasis
    Alana L Welm; Fiscal Year: 2011
    ..Moreover, several large clinical trials performed with heparin or low molecular weight heparin have shown that survival is significantly improved by daily subcutaneous administration of heparin or ..
  23. Regulation of TGF-beta Signaling and Embryonic Development by GTPases
    Gerald H Thomsen; Fiscal Year: 2010
    ..of small GTPases such as Ras and RhoA are relatively well understood, but there exists a variety of large molecular weight GTPases whose functions are poorly characterized...
  24. TNF and Mitochondrial Dysfunction in ALD
    Craig J McClain; Fiscal Year: 2010
    ..Cytokines are low molecular weight mediators of cellular communication that are produced and released by numerous cell types such as monocytes, ..
  25. Protein Handling By Renal Proximal Tubule Epithelial Cells
    Nicholas Ferrell; Fiscal Year: 2013
    ..factors: (1) changes in shear stress resulting from altered tubular flow rates and (2) infiltration of large molecular weight proteins into the proximal tubule, both of which may be present in disease, affect the kinetics and outcome ..
  26. Role of H-Ras in Retinal Cell Death in Diabetes
    Renu A Kowluru; Fiscal Year: 2013
    ..of diabetic retinopathy, matrixmetalloproteinase-9 (MMP-9) is activated in the retina via a small molecular weight G-protein, H-Ras, activated MMP-9 damages mitochondria and accelerates retinal capillary cell apoptosis, and ..
  27. Reactivation of Fetal Gamma-globin Genes for the Treatment of Beta-globin Disorde
    Osamu Tanabe; Fiscal Year: 2010
    ..abstract_text> ..
  28. Development of Transdermal Films for the the Delivery of HIV Therapeutics
    Anthony Sang Won Ham; Fiscal Year: 2010 developing IQP-0410, a highly potent nonnucleoside pyrimidinedione (PYD) inhibitor of HIV-1 with a molecular weight of 352 g/mole...
  29. The role of trace metals and T-channels in pain
    Slobodan M Todorovic; Fiscal Year: 2013
    ..via H191, and loss of the "Substance T" effect in H191Q mutants, we hypothesize that "Substance T" is a low molecular weight peptide that can act as a chelator of trace metals that can increase cellular excitability of nociceptive DRG ..
  30. Biology of the HMW1 and HMW2 Adhesins of H. Influenzae
    JOSEPH W ST. GEME; Fiscal Year: 2013
    ..influenzae disease. More generally, these studies will provide fundamental insights into host-microbial relationships, bacterial protein secretion, and prokaryotic protein glycosylation. ..
  31. Role of Neuropeptide Y in Obesity-Receptor Specific Analogs
    Ambikaipakan Balasubramaniam; Fiscal Year: 2013
    ..Our investigations towards this goal have already led to the identification of lower molecular weight tri-peptide (Y5), pentapeptide (Y1 and Y4) and decapeptide (Y2) motifs for interaction with NPY receptors...
  32. The Role of Beta-Arrestins 1 and 2 in Rheumatoid Arthritis
    Hongkuan Fan; Fiscal Year: 2010
    ..It was also demonstrated that LPS- and the endogenous TLR4 ligand low molecular weight hyaluronan (LMW-HA)- induced TNF, IL-6 and IL-10 production were augmented in splenocytes from - arrestin 2 ..
  33. Development of Small Heat Shock Proteins as Therapeutic Agents in the Eye
    JONATHAN MARK PETRASH; Fiscal Year: 2010
    ..aim, we will test the effectiveness of sHSP delivered via nanoparticles at suppressing and/or reversing high molecular weight aggregates formed from mutant proteins associated with RP and cataracts...
  34. Novel Role of a Nucleoporin Gene in Atrial Fibrillation, the Most Common Cardiac
    Qing Kenneth Wang; Fiscal Year: 2013
    ..complex of about 30 nucleoporins, and plays a key role in bi-directional transport of macromolecules with a molecular weight of >40 kDa across the nucleus membrane...
    Herbert Samuels; Fiscal Year: 2009
    ..Based on chromatographic analysis we have identified high and low molecular weight (MW) NIF-1 complexes. The high MW (1-~2 MDa) complex contains the novel factors EMSY, DBC-1 and Ki-67...
  36. Design of CNS-Permeable Agents for the Treatment of Lipid Storage Diseases
    Scott D Larsen; Fiscal Year: 2010
    ..Toward these objectives, selected properties of the PDMP template (e.g. molecular weight, polar surface area and number of rotatable bonds) will be modified to more closely approximate those of CNS ..
  37. Development and testing of polyvalent anthrax toxin inhibitors
    JEREMY S MOGRIDGE; Fiscal Year: 2012
    ..of PLGA-based and liposome-based inhibitors that exhibit high potencies in vitro and that differ in size (molecular weight of PLGA;diameter of liposomes), lipid composition (homogeneous and phase-separated liposomes) and/or peptide ..
  38. Residual Kidney Function in ESRD: Measurements, Serum Markers and Outcomes
    Tariq Shafi; Fiscal Year: 2013
    ..a radiologic contrast agent referred to as the new standard for measuring GFR, (2) test if two endogenous low molecular weight proteins (beta-trace protein [BTP] and cystatin C), that are excellent serum markers of GFR but not removed ..
  39. Recombinant Polymers for Transarterial Chemoembolization of Liver Cancer
    AZADEH POURSAID; Fiscal Year: 2013
    ..embolic agent capable of retaining and releasing a wider range of therapeutics both in chemistry and molecular weight, capable of controlled degradation into non-toxic amino acids, and capable of superior tumor penetration...
  40. Regulation and Function of Adiponectin Oligomerization
    Feng Liu; Fiscal Year: 2012
    ..Circulating adiponectin exists as trimer, hexamer, and high molecular weight species which activate divergent signaling pathways and exert distinct biological functions on their target ..
  41. Impact of Estrogens and Menopause on Interacting Monoamine Neurotransmitters in t
    Jeffrey K Yao; Fiscal Year: 2013
    ..Yao's (co-PI) laboratory has focused on developing technologies to quantify multiple low-molecular weight redox-active compounds (e.g...
  42. Developing diagnostic and therapeutic stem cells for cancer therapy
    Khalid A Shah; Fiscal Year: 2013
    ..with Henegouwen lab (Utrecht University, The Netherlands) which is leading efforts in developing low molecular weight, highly soluble EGFR specific nanobodies (EGFR-NB), we have recently shown that mammalian cells can be ..
  43. PSMA-based Cancer Imaging Agents
    Martin G Pomper; Fiscal Year: 2013
    ..We propose to expand upon our successful low molecular weight, urea-based series of imaging agents for PCa...
  44. BETR Therapy for Herpesvirus-Associated Tumors
    Martin G Pomper; Fiscal Year: 2013
    ..therapy for EBV-associated malignancies that relies on the metabolic concentration of a radiolabeled low molecular weight substrate in tumors mediated by the EBV thymidine kinase (TK)...
  45. Development of a Chloroquine Replacement Drug
    MICHAEL KEVIN RISCOE; Fiscal Year: 2010
    ..are considered highly desirable in a new antimalarial - ease of synthesis, low cost of goods, achiral, low molecular weight (e.g...
  46. Proteomics of Cell Death via 2-D Microfluidic Profiling
    DON DEVOE; Fiscal Year: 2009 for the rapid profiling of protein modifications based on changes in isoelectric point (pi) and molecular weight (MW) during programmed cell death, and identification of modified proteins via mass spectrometry...
  47. Oligosaccharide moieties of human milk glycans that inhibit pathogens
    David S Newburg; Fiscal Year: 2013
    ..oral inoculation, and we discovered that these pathogenic viruses are strongly inhibited by specific high molecular weight human milk glycans in vitro...
  48. Discovery of novel anti-HBV compounds targeting host factors
    Pavel A Petukhov; Fiscal Year: 2013
    ..To achieve this goal, our specific aims are as follo: Aim 1. Identify lead- or drug-like low molecular weight inhibitors of HBV nucleocapsid promoter activity using high- throughput screening of chemical libraries with ..
  49. Novel Biomarkers Predictive of Heparin-Induced Thrombocytopenia
    Barbara A Konkle; Fiscal Year: 2010
    ..Thrombosis is the major cause of death in the United States and heparin, in unfractionated (UFH) or low molecular weight form, is uniformly used for anticoagulation in the hospital setting...
  50. Human Macrophage Sodium Channels: Novel Targets for Inflammatory Diseases
    Michael D Carrithers; Fiscal Year: 2013
    ..sodium channels are attractive pharmacologic targets because their function can be blocked by low molecular weight, orally active drugs that are currently used clinically to treat epilepsy...
  51. Phthalates in Drugs and Male Genital Malformation
    Allen A Mitchell; Fiscal Year: 2012
    ..However, it is not widely appreciated that manufacturers use low molecular weight phthalates (e.g...
  52. Prevention of diabetes by pDNA encoded with IL-10
    Jagdish Singh; Fiscal Year: 2010
    ..The copolymers will be characterized for weight average molecular weight by gel permeation 1 chromatography and number average molecular weight by H NMR. (2)...
  53. Coordinate Regulation Of Nuclear DGK-theta and PLD
    DANIEL RABEN; Fiscal Year: 2009
    ..We find that the small molecular weight GTP-binding protein RhoA reciprocally regulates two signaling enzymes, diacylglycerol kinase-theta and ..
    Michael Rod Zalutsky; Fiscal Year: 2013
    ..precursor to the mAb prior to the labeling reaction;3) investigate the nature of the low and high molecular weight labeled catabolites generated in tumor cells in vitro and tumor and normal tissues in vivo;4) evaluate the ..
  55. Investigating the role of Bacillithiol in metal ion homeostasis and the reactive
    Pete Chandrangsu; Fiscal Year: 2013
    ..DESCRIPTION (provided by applicant): The overall goal of this application is to understand the role of the low molecular weight (LMW) thiol, bacillithiol (BSH), in bacterial physiology using a genetic and biochemical approach...
  56. Regulation of Angiogenesis by Kininogen
    Keith R McCrae; Fiscal Year: 2013
    ..Recent studies in our laboratory have focused on the mechanisms by which cleaved high molecular weight kininogen (HKa), a member of the intrinsic coagulation pathway, induces apoptosis of proliferating ..
  57. Intermittent hypoxia, adiponectin, and insulin resistance in cardiovascular risk
  58. Characterization of the role of gap junction proteins in ER stress and obesity
    Amir Tirosh; Fiscal Year: 2013
  59. Proeolytic Processing of Cyclin E in Breast Cancer
    Khandan Keyomarsi; Fiscal Year: 2013
    ..proteins in the initiation and proliferation of tumors in the mammary gland, with an emphasis on the low molecular weight forms of cyclin E (LMW-E)...
  60. Role of Cholesterol in Age-related Decline in Steroidogenesis
    Salman Azhar; Fiscal Year: 2013
    ..We also test a second hypothesis that pharmacological intervention with the use of small molecular weight synthetic antioxidants that reduce the degree of oxidative damage will prevent, reverse, or attenuate the age-..
  61. Engineering DNA Delivery Polymers using Combinatorial and Cheminformatics Methods
    Kaushal Rege; Fiscal Year: 2013
    ..g. molecular weight, hydrophobicity, zeta potential, etc...
  62. Interactions between Bacterial Tyrosine Kinases and Phosphatases
    Ranajeet Ghose; Fiscal Year: 2013
    ..The YC is autophosphorylated in an intermolecular fashion and dephosphorylated by low molecular weight protein tyrosine phosphatases (LMW-PTP)...
    Robert Colman; Fiscal Year: 2001
    ..of the contact system (CS) in plasma produces plasma kallikrein which activates neutrophils, cleaves high molecular weight kininogen (HK) and releases bradykinin which induces pain, swelling, diarrhea, and muscle contraction, all of ..
  64. Passivating Proteins in Implantable Glucose Sensors
    W Ward; Fiscal Year: 2004
    ..The proteins will include von Willebrand factor, high molecular weight kininogen, albumin, and hemoglobin...
  65. Long Circulating Low Molecular Weight Heparins Pulmonary
    Fakhrul Ahsan; Fiscal Year: 2006
    ..Because of the limitations of traditional anti-coagulant therapy for VTE, low molecular weight heparin (LMWH), which are smaller fragments of unfractionated heparin, has recently been used as a drug of ..
  66. Tropomyosin in the antiangiogenic activity of HKa
    Keith McCrae; Fiscal Year: 2007
    High molecular weight kininogen (HK) is an abundant plasma glycoprotein that plays a central role in the kallikrein-kinin system...
  67. Mucus-penetrating nanoparticles for sustained microbicide delivery
    Justin Hanes; Fiscal Year: 2009
    ..By densely coating MPP with low molecular weight polyethylene glycol (PEG) we found that unexpectedly large MPP 100-500 nm in diameter can be engineered to ..
  68. Regulation of neurotrophin expression in the periphery
    LORI ISAACSON; Fiscal Year: 2009
    ..We recently showed that there is little mature nerve growth factor (NGF) and that high molecular weight (MW) NGF species are predominant in peripheral neurons and target tissues...
  69. Screening for Chemicals that Potentiate TRAIL-Induced Apoptosis of Cancer Cells
    DMITRI ROZANOV; Fiscal Year: 2007
    ..Our Specific Aims are: (1) To identify low molecular weight chemicals that can efficiently enhance TRAIL-mediated apoptosis in human fibrosarcoma HT1080 cells...
    Richard Mayne; Fiscal Year: 1992
    ..two pepsin-resistant fragments of type IX collagen were isolated from chicken sterna and called the high molecular weight and low molecular weight components or HMW and LMW...
    Kenneth Dorshkind; Fiscal Year: 1991
    ..One, termed S17, supports B cell differentiation while another, S10, does not. The molecular weight of a factor secreted by S17 that induces Ig expression in pre-B cells will be determined by high pressure ..