spermatogenesis

Summary

Summary: The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA, SPERM.

Top Publications

  1. ncbi Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting
    Masahiro Kaneda
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 429:900-3. 2004
  2. ncbi A germline-specific class of small RNAs binds mammalian Piwi proteins
    Angelique Girard
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 442:199-202. 2006
  3. ncbi A novel class of small RNAs bind to MILI protein in mouse testes
    Alexei Aravin
    Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Nature 442:203-7. 2006
  4. ncbi Essential role of Plzf in maintenance of spermatogonial stem cells
    Jose A Costoya
    Cancer Biology and Genetics Program, Department of Pathology Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Genet 36:653-9. 2004
  5. ncbi The murine testicular transcriptome: characterizing gene expression in the testis during the progression of spermatogenesis
    James E Shima
    Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
    Biol Reprod 71:319-30. 2004
  6. pmc Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
    Hiroshi Kubota
    Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 101:16489-94. 2004
  7. ncbi Regulation of cell fate decision of undifferentiated spermatogonia by GDNF
    X Meng
    Research Programs of Developmental Biology, Molecular Neurobiology, Electron Microscopy Unit, Institute of Biotechnology, Viikki Biocenter, Finland
    Science 287:1489-93. 2000
  8. pmc MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Dev Cell 20:364-75. 2011
  9. pmc piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling
    Huiyan Huang
    Department of Pharmacology, Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794 5140, USA
    Dev Cell 20:376-87. 2011
  10. pmc Stage-specific expression profiling of Drosophila spermatogenesis suggests that meiotic sex chromosome inactivation drives genomic relocation of testis-expressed genes
    Maria D Vibranovski
    Department of Ecology, The University of Chicago, Chicago, Illinois, United States of America
    PLoS Genet 5:e1000731. 2009

Detail Information

Publications407 found, 100 shown here

  1. ncbi Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting
    Masahiro Kaneda
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 429:900-3. 2004
    ..Dnmt3a conditional mutant males show impaired spermatogenesis and lack methylation at two of three paternally imprinted loci examined in spermatogonia...
  2. ncbi A germline-specific class of small RNAs binds mammalian Piwi proteins
    Angelique Girard
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 442:199-202. 2006
    ..Although their function must still be resolved, the abundance of piRNAs in germline cells and the male sterility of Miwi mutants suggest a role in gametogenesis...
  3. ncbi A novel class of small RNAs bind to MILI protein in mouse testes
    Alexei Aravin
    Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Nature 442:203-7. 2006
    ..for germ- and stem-cell development in invertebrates, and two Piwi members--MILI and MIWI--are essential for spermatogenesis in mouse...
  4. ncbi Essential role of Plzf in maintenance of spermatogonial stem cells
    Jose A Costoya
    Cancer Biology and Genetics Program, Department of Pathology Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Genet 36:653-9. 2004
    ..Here we show that Zfp145, encoding the transcriptional repressor Plzf, has a crucial role in spermatogenesis. Zfp145 expression was restricted to gonocytes and undifferentiated spermatogonia and was absent in tubules ..
  5. ncbi The murine testicular transcriptome: characterizing gene expression in the testis during the progression of spermatogenesis
    James E Shima
    Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
    Biol Reprod 71:319-30. 2004
    ..insight into the activities, biological and chronological, of testicular cell types during the progression of spermatogenesis. Further analysis of this time course can provide a distinct and more definitive view into the genes ..
  6. pmc Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
    Hiroshi Kubota
    Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 101:16489-94. 2004
    ..Under these conditions, SSCs proliferated over 6 months, reconstituted long-term spermatogenesis after transplantation into recipient testes, and restored fertility to infertile recipients...
  7. ncbi Regulation of cell fate decision of undifferentiated spermatogonia by GDNF
    X Meng
    Research Programs of Developmental Biology, Molecular Neurobiology, Electron Microscopy Unit, Institute of Biotechnology, Viikki Biocenter, Finland
    Science 287:1489-93. 2000
    ..cells, regulates cell fate decisions of undifferentiated spermatogonial cells that include the stem cells for spermatogenesis. Gene-targeted mice with one GDNF-null allele show depletion of stem cell reserves, whereas mice ..
  8. pmc MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Dev Cell 20:364-75. 2011
    ..The mice display meiotic arrest during spermatogenesis, demethylation and derepression of retrotransposons, and defects in primary piRNA biogenesis...
  9. pmc piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling
    Huiyan Huang
    Department of Pharmacology, Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794 5140, USA
    Dev Cell 20:376-87. 2011
    ..We propose that mitochondrial-surface PA generated by MitoPLD/Zuc recruits or activates nuage components critical for piRNA production...
  10. pmc Stage-specific expression profiling of Drosophila spermatogenesis suggests that meiotic sex chromosome inactivation drives genomic relocation of testis-expressed genes
    Maria D Vibranovski
    Department of Ecology, The University of Chicago, Chicago, Illinois, United States of America
    PLoS Genet 5:e1000731. 2009
    ..melanogaster spermatogenesis: mitosis, meiosis, and post-meiosis...
  11. ncbi Meiotic sex chromosome inactivation
    James M A Turner
    Division of Stem Cell Biology and Developmental Genetics, MRC NIMR, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Development 134:1823-31. 2007
    ..However, another form of X-inactivation takes place in the male, during spermatogenesis, as germ cells enter meiosis...
  12. pmc Evolution in the fast lane: rapidly evolving sex-related genes in Drosophila
    Wilfried Haerty
    Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
    Genetics 177:1321-35. 2007
    ..Several testis-specific genes, male seminal fluid proteins (SFPs), and spermatogenesis genes show lineage-specific bursts of accelerated evolution and positive selection...
  13. pmc Stra8 and its inducer, retinoic acid, regulate meiotic initiation in both spermatogenesis and oogenesis in mice
    Ericka L Anderson
    Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 105:14976-80. 2008
    ..We conclude that, in mice, Stra8 regulates meiotic initiation in both spermatogenesis and oogenesis...
  14. pmc Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans
    Colin C Conine
    The Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01606, USA
    Proc Natl Acad Sci U S A 107:3588-93. 2010
    ..double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate numerous spermatogenesis-expressed mRNAs...
  15. pmc Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes
    Toshiaki Watanabe
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Genes Dev 20:1732-43. 2006
    ..gsRNAs are expressed during spermatogenesis in a developmentally regulated manner, are mapped to the genome in clusters, and have strong strand bias...
  16. ncbi Mili, a mammalian member of piwi family gene, is essential for spermatogenesis
    Satomi Kuramochi-Miyagawa
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita Shi, Osaka 565 0871, Japan
    Development 131:839-49. 2004
    ..b>Spermatogenesis in the MILI-null mice was blocked completely at the early prophase of the first meiosis, from the zygotene to ..
  17. ncbi Role of the Dnmt3 family in de novo methylation of imprinted and repetitive sequences during male germ cell development in the mouse
    Yuzuru Kato
    Department of Integrated Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan
    Hum Mol Genet 16:2272-80. 2007
    ..Finally, all these sequences showed moderate to severe hypomethylation in Dnmt3L-deficient prospermatogonia, indicating the critical function and broad specificity of this factor in de novo methylation...
  18. ncbi Bax-deficient mice with lymphoid hyperplasia and male germ cell death
    C M Knudson
    Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 270:96-9. 1995
    ..Multinucleated giant cells and dysplastic cells accompanied massive cell death. Thus, the loss of Bax results in hyperplasia or hypoplasia, depending on the cellular context...
  19. ncbi PATZ1 gene has a critical role in the spermatogenesis and testicular tumours
    M Fedele
    IEOS, CNR and Dipartimento di Biologia e Patologia Cellulare e Molecolare, Universita di Napoli Federico II, 80131 Naples, Italy
    J Pathol 215:39-47. 2008
    ..Male PATZ1(-/-) mice were unfertile, suggesting a crucial role of this gene in spermatogenesis. Consistently, most of adult testes from these mice showed only few spermatocytes, associated with increased ..
  20. pmc Copy number variants in patients with severe oligozoospermia and Sertoli-cell-only syndrome
    Frank Tüttelmann
    Institute of Human Genetics, University of Munster, Munster, Germany
    PLoS ONE 6:e19426. 2011
    ..The recurring, patient-specific and private, sex-chromosomal CNVs as well as those associated with SCOS are candidates for further, larger case-control and re-sequencing studies...
  21. pmc MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis
    Shane T Grivna
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 103:13415-20. 2006
    ..Together, our results indicate that MIWI, a PIWI subfamily protein, uses piRNA as the major, but not exclusive, binding partner, and it is associated with translational machinery...
  22. pmc The blood-testis barrier and its implications for male contraception
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Pharmacol Rev 64:16-64. 2012
    ..Instead, it undergoes extensive restructuring during the seminiferous epithelial cycle of spermatogenesis at stage VIII to allow the transit of preleptotene spermatocytes at the BTB...
  23. ncbi Male germ cell gene expression
    Edward M Eddy
    Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709 2233, USA
    Recent Prog Horm Res 57:103-28. 2002
    ..The process of spermatogenesis occurs in overlapping waves, with cohorts of germ cells developing in synchrony...
  24. ncbi In vitro production of functional sperm in cultured neonatal mouse testes
    Takuya Sato
    Department of Urology, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    Nature 471:504-7. 2011
    b>Spermatogenesis is one of the most complex and longest processes of sequential cell proliferation and differentiation in the body, taking more than a month from spermatogonial stem cells, through meiosis, to sperm formation...
  25. ncbi MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline
    Michelle A Carmell
    Cold Spring Harbor Laboratory, Howard Hughes Medical Institute, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Dev Cell 12:503-14. 2007
    ..In mice, two Piwi-family members have been demonstrated to have essential roles in spermatogenesis. Here, we examine the effects of disrupting the gene encoding the third family member, MIWI2...
  26. pmc A multitude of genes expressed solely in meiotic or postmeiotic spermatogenic cells offers a myriad of contraceptive targets
    Nikolaus Schultz
    Cecil H and Ida Green Center for Reproductive Biology Sciences, Howard Hughes Medical Institute and Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 100:12201-6. 2003
    ..This extensive group provides a plethora of potential targets for germ cell-directed contraception and a staggering number of candidate proteins that could be critical for fertilization...
  27. ncbi Functional coherence of the human Y chromosome
    B T Lahn
    Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 278:675-80. 1997
    ..The coherence of the NRY's gene content contrasts with the apparently haphazard content of most eukaryotic chromosomes...
  28. pmc Acetylation-mediated proteasomal degradation of core histones during DNA repair and spermatogenesis
    Min Xian Qian
    Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, and College of Life Sciences, Beijing Normal University, 19 XinJieKouWai Avenue, Beijing 100875, China
    Cell 153:1012-24. 2013
    ..Thus, PA200/Blm10 specifically targets the core histones for acetylation-mediated degradation by proteasomes, providing mechanisms by which acetylation regulates histone degradation, DNA repair, and spermatogenesis.
  29. ncbi Proliferation and functional maturation of Sertoli cells, and their relevance to disorders of testis function in adulthood
    Richard M Sharpe
    MRC Human Reproductive Sciences Unit, Centre for Reproductive Biology, The Chancellor s Building, University of Edinburgh, 49 Little France Crescent, Old Dalkeith Road, Edinburgh EH16 4SB, UK
    Reproduction 125:769-84. 2003
    ..Failure of Sertoli cells to mature, with consequent inability to express functions capable of supporting spermatogenesis, is a prime example...
  30. pmc Bromodomain-dependent stage-specific male genome programming by Brdt
    Jonathan Gaucher
    INSERM, U823, Universite Joseph Fourier Grenoble 1, Institut Albert Bonniot, Grenoble, France
    EMBO J 31:3809-20. 2012
    ....
  31. pmc Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse
    Ergin Beyret
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    Dev Biol 355:215-26. 2011
    ..proteins in the adult, we show that PIWI proteins and presumably piRNAs in the adult are required only for spermatogenesis. Spermatocytes without PIWI proteins are arrested at the pachytene stage, when the sex chromosomes undergo ..
  32. pmc Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway
    Ke Zheng
    Center for Animal Transgenesis and Germ Cell Research and Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 107:11841-6. 2010
    ..The Mov10l1 mutant males are sterile owing to complete meiotic arrest. This mouse mutant expresses Piwi proteins but lacks piRNAs, suggesting that MOV10L1 is required for piRNA biogenesis and/or loading to Piwi proteins...
  33. ncbi Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocr Rev 25:747-806. 2004
    b>Spermatogenesis is the process by which a single spermatogonium develops into 256 spermatozoa, one of which will fertilize the ovum...
  34. ncbi Postmeiotic sex chromatin in the male germline of mice
    Satoshi H Namekawa
    Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Boston, 02114, USA
    Curr Biol 16:660-7. 2006
    ..An abundance of X-linked spermatogenesis genes has spawned the view that the X must be active ...
  35. pmc Regulation of spermatogonial stem cell self-renewal in mammals
    Jon M Oatley
    Department of Animal Sciences, Center for Reproductive Biology and Health, College of Agricultural Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Annu Rev Cell Dev Biol 24:263-86. 2008
    Mammalian spermatogenesis is a classic adult stem cell-dependent process, supported by self-renewal and differentiation of spermatogonial stem cells (SSCs)...
  36. ncbi The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements
    Serena De Fazio
    European Molecular Biology Laboratory, Mouse Biology Unit, Via Ramarini 32, Monterotondo Scalo 00015, Italy
    Nature 480:259-63. 2011
    ..In addition, the hallmarks of piRNA amplification are observed in Miwi2-deficient gonadocytes. We conclude that cycles of intra-Mili secondary piRNA biogenesis fuel piRNA amplification that is absolutely required for LINE1 silencing...
  37. ncbi Flow cytometric characterization of viable meiotic and postmeiotic cells by Hoechst 33342 in mouse spermatogenesis
    Henri Bastos
    Laboratoire de Gametogenèse, Apoptose et Génotoxicité, DRR DSV CEA, U566 INSERM, Universite Paris 7, Fontenay aux Roses, France
    Cytometry A 65:40-9. 2005
    b>Spermatogenesis in adult is a complex stepwise process leading to terminally differentiated spermatozoa. The cellular heterogeneity of testis renders complex the studies on molecular aspects of this differentiation process...
  38. ncbi Impaired meiotic DNA-damage repair and lack of crossing-over during spermatogenesis in BRCA1 full-length isoform deficient mice
    Xiaoling Xu
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Development 130:2001-12. 2003
    ..Here we show that mouse Brca1 is required for DNA-damage repair and crossing-over during spermatogenesis. Male Brca1(Delta11/Delta11)p53(+/-) mice that carried a homozygous deletion of Brca1 exon 11 and a p53 ..
  39. ncbi Paternal DNA packaging in spermatozoa: more than the sum of its parts? DNA, histones, protamines and epigenetics
    David Miller
    Division of Reproduction and Early Development, Leeds Institute of Genetics, Health and Therapeutics, University of Leeds, Clarendon Way, Leeds LS2 9JT, UK
    Reproduction 139:287-301. 2010
    ..We also provide some thoughts on why this signature may be required in early embryogenesis...
  40. ncbi Spermatogenesis and cycle of the seminiferous epithelium
    Rex A Hess
    Department of Veterinary Biosciences, University of Illinois, Urbana, IL 61802 6199, USA
    Adv Exp Med Biol 636:1-15. 2008
    b>Spermatogenesis is a complex biological process of cellular transformation that produces male haploid germ cells from diploid spermatogonial stem cells...
  41. pmc Functional hierarchy and reversibility within the murine spermatogenic stem cell compartment
    Toshinori Nakagawa
    Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Science 328:62-7. 2010
    ..In mice, it is believed that a homogeneous stem cell population of single spermatogonia supports spermatogenesis, and that differentiation, which is accompanied by the formation of connected cells (cysts) of increasing ..
  42. pmc Foxo1 is required in mouse spermatogonial stem cells for their maintenance and the initiation of spermatogenesis
    Meredith J Goertz
    Department of Pathology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390 9072, USA
    J Clin Invest 121:3456-66. 2011
    Spermatogonial stem cells (SSCs) capable of self-renewal and differentiation are the foundation for spermatogenesis. Although several factors important for these processes have been identified, the fundamental mechanisms regulating SSC ..
  43. ncbi DNMT1 and HDAC1 gene expression in impaired spermatogenesis and testicular cancer
    Olufunmilade A Omisanjo
    Department of Urology and Pediatric Urology, Giessen, Germany
    Histochem Cell Biol 127:175-81. 2007
    ..deacetylases (HDACs) play an important role for the regulation of gene expression during carcinogenesis and spermatogenesis. We therefore studied the cell-specific expression of DNMT1 and HDAC1 for the first time in human testicular ..
  44. pmc Cre recombinase activity specific to postnatal, premeiotic male germ cells in transgenic mice
    Patricia I Sadate-Ngatchou
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington
    Genesis 46:738-42. 2008
    ..The postnatal, premeiotic, male germ cell-specific activity of Stra8-cre makes this mouse line a unique resource to study testicular germ cell development...
  45. ncbi Glial cell-line derived neurotrophic factor-mediated RET signaling regulates spermatogonial stem cell fate
    Cathy K Naughton
    Division of Urology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biol Reprod 74:314-21. 2006
    Normal spermatogenesis is essential for reproduction and depends on proper spermatogonial stem cell (SSC) function. Genes and signaling pathways that regulate SSC function have not been well defined...
  46. ncbi The RNA-binding protein NANOS2 is required to maintain murine spermatogonial stem cells
    Aiko Sada
    Department of Genetics, SOKENDAI, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Science 325:1394-8. 2009
    ..Thus, NANOS2 is a key stem cell regulator that is expressed in self-renewing spermatogonial stem cells and maintains the stem cell state during murine spermatogenesis.
  47. ncbi miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis
    Wei Deng
    Department of Cell Biology, Duke University Medical Center, P O Box 3709, DUMC, Durham, NC 27710, USA
    Dev Cell 2:819-30. 2002
    ..Here we report the cloning of a murine piwi gene (miwi) essential for spermatogenesis. miwi encodes a cytoplasmic protein specifically expressed in spermatocytes and spermatids...
  48. pmc piRNA biogenesis during adult spermatogenesis in mice is independent of the ping-pong mechanism
    Ergin Beyret
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06509, USA
    Cell Res 22:1429-39. 2012
    ....
  49. pmc Reproductive isolation in hybrid mice due to spermatogenesis defects at three meiotic stages
    Ayako Oka
    Transdsciplinary Research Integration Center, Research Organization of Information and Systems, Toranomon, Tokyo, Japan 105 0001
    Genetics 186:339-51. 2010
    ..Furthermore, the stages with disruptions are not correlated with the genetic distance between the respective parental strains...
  50. pmc MVH in piRNA processing and gene silencing of retrotransposons
    Satomi Kuramochi-Miyagawa
    Graduate School of Frontier Biosciences, Osaka University, Osaka 565 0871, Japan
    Genes Dev 24:887-92. 2010
    ..Comprehensive analysis of piRNAs in MVH-deficient fetal male germ cells showed that MVH plays crucial roles in the early phase of the ping-pong amplification cycle...
  51. pmc A young Drosophila duplicate gene plays essential roles in spermatogenesis by regulating several Y-linked male fertility genes
    Yun Ding
    State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
    PLoS Genet 6:e1001255. 2010
    ..Sequence and expression analysis demonstrates that one of the new duplicates, nsr (novel spermatogenesis regulator), exhibits positive selection signals and novel subcellular localization pattern...
  52. ncbi Chromatoid body and small RNAs in male germ cells
    Oliver Meikar
    Department of Physiology, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
    Reproduction 142:195-209. 2011
    ..Several microRNAs have been identified during spermatogenesis. In addition, a high number of piRNAs are present both in embryonic and postnatal male germ cells, with their ..
  53. pmc A local autocrine axis in the testes that regulates spermatogenesis
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Nat Rev Endocrinol 6:380-95. 2010
    ..will discuss this important autocrine-based cellular axis that parallels the hormonal-based hypothalamic-pituitary-testicular axis that regulates spermatogenesis. This local regulatory axis is the emerging target for male contraception.
  54. ncbi The Sertoli cell cytoskeleton
    A Wayne Vogl
    Department of Cellular and Physiological Sciences, The University of British Columbia, Vancouver, British Columbia, Canada
    Adv Exp Med Biol 636:186-211. 2008
    ..filaments and microtubules have distinct patterns of distribution that change during the cyclic process of spermatogenesis. Each of the three major cytoskeletal elements is either concentrated at or related in part to intercellular ..
  55. pmc The RNase III enzyme DROSHA is essential for microRNA production and spermatogenesis
    Qiuxia Wu
    Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada 89557, USA
    J Biol Chem 287:25173-90. 2012
    ..To investigate the role of canonical miRNA and/or endogenous siRNA production in spermatogenesis, we generated Drosha or Dicer conditional knock-out (cKO) mouse lines by inactivating Drosha or Dicer ..
  56. ncbi Histological evaluation of the human testis--approaches to optimizing the clinical value of the assessment: mini review
    R I McLachlan
    Prince Henry s Institute, Monash University, Clayton, Victoria, Australia
    Hum Reprod 22:2-16. 2007
    ..We propose a new diagnosis code for testicular biopsies that addresses the needs of ART clinicians and allows data storage and retrieval of value in clinical practice and research...
  57. ncbi Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats
    Mahitosh Sarkar
    Reproductive Physiology Unit, Dept of Physiology, University Colleges of Science and Technology, Calcutta University, 92 Acharya Prafulla Chandra Road, Calcutta 700009, India
    Asian J Androl 5:27-31. 2003
    To investigate the effect of arsenic on spermatogenesis.
  58. ncbi Conserved role of nanos proteins in germ cell development
    Masayuki Tsuda
    Division of Mammalian Development, National Institute of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411 8540, Japan
    Science 301:1239-41. 2003
    ..Hence, although mice and flies differ in their mechanisms for germ cell specification, there seems to be conserved function for nanos proteins among invertebrates and vertebrates...
  59. ncbi Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila
    Christina Rathke
    Philipps Universitat Marburg, Fachbereich Biologie, Entwicklungsbiologie, 35043 Marburg, Germany
    J Cell Sci 120:1689-700. 2007
    ..Finally, we show that histone modifications and removal are independent of protamine synthesis...
  60. pmc Extensive meiotic asynapsis in mice antagonises meiotic silencing of unsynapsed chromatin and consequently disrupts meiotic sex chromosome inactivation
    Shantha K Mahadevaiah
    Division of Stem Cell Biology and Developmental Genetics, Medical Research Council National Institute for Medical Research, London NW7 1AA, England, UK
    J Cell Biol 182:263-76. 2008
    ..Apoptosis does not occur in mice with a single additional asynapsed chromosome with unrepaired meiotic DSBs and no disturbance of MSCI...
  61. ncbi Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility
    T R Kumar
    Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Genet 15:201-4. 1997
    ..Although FSH was predicted to be necessary for spermatogenesis and Sertoli cell growth in males, FSH-deficient males are fertile despite having small testes...
  62. ncbi Tyro-3 family receptors are essential regulators of mammalian spermatogenesis
    Q Lu
    Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 398:723-8. 1999
    ..Additional observations indicate that these receptors may also be essential for the tropic maintenance of diverse cell types in the mature nervous, immune and reproductive systems...
  63. ncbi Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile
    Michael Reuter
    European Molecular Biology Laboratory, Grenoble Outstation, France
    Nat Struct Mol Biol 16:639-46. 2009
    ..Tudor domain-containing protein-1 (Tdrd1) as a factor specifically associated with the Mili piRNP throughout spermatogenesis. Complex formation is promoted by the recognition of symmetrically dimethylated arginines at the N terminus ..
  64. ncbi Xist-deficient mice are defective in dosage compensation but not spermatogenesis
    Y Marahrens
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genes Dev 11:156-66. 1997
    ..Xist gene encodes a large untranslated RNA that has been implicated in mammalian dosage compensation and in spermatogenesis. To investigate the function of the Xist gene product, we have generated male and female mice that carry a ..
  65. ncbi Positional cloning and characterization of mouse mei8, a disrupted allelle of the meiotic cohesin Rec8
    Laura A Bannister
    The Jackson Laboratory, Bar Harbor, Maine, USA
    Genesis 40:184-94. 2004
    ..Affected males have small testes due to arrest of spermatogenesis during meiotic prophase I...
  66. pmc MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs
    Robert J A Frost
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Proc Natl Acad Sci U S A 107:11847-52. 2010
    ..of LTR and LINE-1 retrotransposons, followed by cell death, causing male infertility and a complete block of spermatogenesis at early prophase of meiosis I...
  67. ncbi CDH1 is a specific marker for undifferentiated spermatogonia in mouse testes
    Masutaka Tokuda
    Division of Cell Biology, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470 1192, Japan
    Biol Reprod 76:130-41. 2007
    In the mammalian testis, spermatogenesis is initiated from a subset of stem cells belonging to undifferentiated type A spermatogonia...
  68. pmc Phenotyping male infertility in the mouse: how to get the most out of a 'non-performer'
    Claire L Borg
    Department of Anatomy and Developmental Biology, The School of Biomedical Sciences, Monash University, Clayton 3800, Australia
    Hum Reprod Update 16:205-24. 2010
    ..The review aims to act as a guide to the 'non-reproductive biologist' to facilitate a systematic analysis of sterile or subfertile mice and to assist in extracting the maximum amount of information from each model...
  69. ncbi Androgen receptor function is required in Sertoli cells for the terminal differentiation of haploid spermatids
    Robert W Holdcraft
    University of Washington School of Medicine, Department of Genome Sciences, Seattle, WA 98195, USA
    Development 131:459-67. 2004
    ..function is required for male embryonic sexual differentiation, pubertal development and the regulation of spermatogenesis in mammals...
  70. pmc X chromosome inactivation during Drosophila spermatogenesis
    Winfried Hense
    Department of Biology II, University of Munich, Planegg Martinsried, Germany
    PLoS Biol 5:e273. 2007
    ..It has been proposed that inactivation of the X chromosome during spermatogenesis contributes to these patterns: genes with a beneficial function late in spermatogenesis should be selectively ..
  71. ncbi Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 2: changes in spermatid organelles associated with development of spermatozoa
    Louis Hermo
    Faculty of Medicine, Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 2B2
    Microsc Res Tech 73:279-319. 2010
    ..During spermiogenesis, many genes/proteins have been implicated in the diverse dynamic events occurring at this time of development of germ cells and the absence of some of these have been shown to result in subfertility or infertility...
  72. ncbi Spermatogenesis in cryptorchidism
    Alexander I Agoulnik
    Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA
    Methods Mol Biol 825:127-47. 2012
    ..Variation in genetic and environmental factors might affect testicular descent. A failure of spermatogenesis and germ cell apoptosis resulting in infertility as well as an increased risk of neoplastic transformation of ..
  73. ncbi New insights to the ubiquitin-proteasome pathway (UPP) mechanism during spermatogenesis
    Cong Cong Hou
    The Sperm Laboratory, College of Life Sciences, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China
    Mol Biol Rep 40:3213-30. 2013
    b>Spermatogenesis is a complicated and highly ordered process which begins with the differentiation of spermatogonial stem cells and ends with the formation of mature sperm...
  74. pmc The fog-3 gene and regulation of cell fate in the germ line of Caenorhabditis elegans
    R E Ellis
    Howard Hughes Medical Institute, Laboratory of Molecular Biology, Madison, Wisconsin, USA
    Genetics 139:561-77. 1995
    In the nematode Caenorhabditis elegans, germ cells normally adopt one of three fates: mitosis, spermatogenesis or oogenesis...
  75. ncbi miR-18, a member of Oncomir-1, targets heat shock transcription factor 2 in spermatogenesis
    Johanna K Björk
    Department of Biosciences, Abo Akademi University, Turku, Finland
    Development 137:3177-84. 2010
    ..in testis, displaying distinct cell-type-specific expression during the epithelial cycle that constitutes spermatogenesis. Expression of HSF2 and of miR-18 exhibit an inverse correlation during spermatogenesis, indicating that, in ..
  76. ncbi Spermatogenesis
    Diana S Chu
    Department of Biology, San Francisco State University, San Francisco, CA, USA
    Adv Exp Med Biol 757:171-203. 2013
    During spermatogenesis, pluripotent germ cells differentiate to become efficient delivery vehicles to the oocyte of paternal DNA...
  77. pmc Notch signaling in Sertoli cells regulates cyclical gene expression of Hes1 but is dispensable for mouse spermatogenesis
    Kazuteru Hasegawa
    Division of Mammalian Development, National Institute of Genetics, Mishima, Shizuoka, Japan
    Mol Cell Biol 32:206-15. 2012
    Mammalian spermatogenesis is a highly regulated system dedicated to the continuous production of spermatozoa from spermatogonial stem cells, and the process largely depends on microenvironments created by Sertoli cells, unique somatic ..
  78. ncbi Notch-1, c-kit and GFRalpha-1 are developmentally regulated markers for premeiotic germ cells
    V von Schönfeldt
    Institute of Reproductive Medicine of the University of Munster, Munster, Germany
    Cytogenet Genome Res 105:235-9. 2004
    ..First attempts and various future perspectives to use spermatogonial stem cells as pathway for the introduction of transgenes are discussed...
  79. pmc Phosphorylation state of a Tob/BTG protein, FOG-3, regulates initiation and maintenance of the Caenorhabditis elegans sperm fate program
    Myon Hee Lee
    The Howard Hughes Medical Institute, Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 108:9125-30. 2011
    ..same animals, sperm fate specification was not only initiated but also maintained, resulting in continuous spermatogenesis in males...
  80. ncbi Expression of Notch pathway components in spermatogonia and Sertoli cells of neonatal mice
    G Dirami
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    J Androl 22:944-52. 2001
    ..ligands in spermatogonia and Sertoli cells indicates that these cells are capable of responding to and eliciting Notch signaling during the process of spermatogenesis. Key words: Cell fate, delta, jagged, presenilin, spermatogenesis.
  81. ncbi Requirement of Notch 1 and its ligand jagged 2 expressions for spermatogenesis in rat and human testes
    T Hayashi
    Department of Urology and Reproductive Medicine, Graduate School, Tokyo Medical and Dental University, Japan
    J Androl 22:999-1011. 2001
    ..However, the role of the Notch signaling system in mammalian spermatogenesis has not been well investigated...
  82. ncbi The role of actin and myosin during spermatogenesis
    Xiao Sun
    The Sperm Laboratory, Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, 388 Yu Hang Tang Road, Hangzhou, 310058, People s Republic of China
    Mol Biol Rep 38:3993-4001. 2011
    b>Spermatogenesis is a transitionary process in which the diploid spermatogonia transform into haploid mature spermatozoa. Actin and myosin have been implicated in various aspects during spermatogenesis...
  83. ncbi In vitro-differentiated embryonic stem cells give rise to male gametes that can generate offspring mice
    Karim Nayernia
    Institute of Human Genetics, University of Gottingen, 37073 Gottingen, Germany
    Dev Cell 11:125-32. 2006
    ..These cells are believed to divide infinitely and to support spermatogenesis throughout life in the male...
  84. pmc piRNA profiling during specific stages of mouse spermatogenesis
    Haiyun Gan
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    RNA 17:1191-203. 2011
    ..mRNAs, and intergenic regions had different length distributions and were differentially regulated in spermatogenesis. piRNA-generating mRNAs (PRMRs), whose expression positively correlated with their piRNA products, ..
  85. pmc Direct action through the sertoli cells is essential for androgen stimulation of spermatogenesis
    P J O'Shaughnessy
    Institute of Comparative Medicine, Division of Cell Sciences, University of Glasgow Veterinary School, Glasgow, UK
    Endocrinology 151:2343-8. 2010
    Androgens act to stimulate spermatogenesis through androgen receptors (ARs) on the Sertoli cells and peritubular myoid cells. Specific ablation of the AR in either cell type will cause a severe disruption of spermatogenesis...
  86. pmc Cul4A is essential for spermatogenesis and male fertility
    Dragana Kopanja
    Department of Biochemistry and Molecular Genetics M C 669, University of Illinois, College of Medicine, 900 S Ashland Ave, Chicago, IL 60607, USA
    Dev Biol 352:278-87. 2011
    ..In this strain, the male mice are infertile and exhibit severe deficiencies in spermatogenesis. The primary spermatocytes are deficient in progression through late prophase I, a time point when expression ..
  87. pmc The E3 ubiquitin ligase Cullin 4A regulates meiotic progression in mouse spermatogenesis
    Yan Yin
    Division of Dermatology, Washington University School of Medicine, St Louis, MO 63110, USA
    Dev Biol 356:51-62. 2011
    ..in the blast cells, largely due to failed degradation of the DNA licensing protein, CDT-1, and premature spermatogenesis. Here we show that inactivation of Cul4a by gene-targeting in mice only affected male but not female ..
  88. pmc Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis
    Takashi Tanaka
    Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 108:10579-84. 2011
    ..bodies assemble as an integrated compartment incorporating both germline and ubiquitous features as spermatogenesis proceeds and that the conserved tudor family genes act as master regulators of this unique RNP remodeling, ..
  89. pmc The key role of vitamin A in spermatogenesis
    Cathryn A Hogarth
    School of Molecular Biosciences, Washington State University, Pullman, WA 99164 7520, USA
    J Clin Invest 120:956-62. 2010
    b>Spermatogenesis in adult mammals is highly organized, with the goal being continual sperm production. Vertebrate testes are arranged into recurring cellular associations that vary with time and distance along the tubule...
  90. ncbi Effects of vasectomy on spermatogenesis and fertility outcome after testicular sperm extraction combined with ICSI
    C M McVicar
    Obstetrics and Gynaecology, School of Medicine, Queen s University Belfast, Institute of Clinical Science, UK
    Hum Reprod 20:2795-800. 2005
    ..The study aims were to compare sperm yields of men post-vasectomy or with obstructive azoospermia (OA) of unknown aetiology with yields of fertile men and to assess any alteration in the clinical pregnancy rates after ICSI...
  91. ncbi Genome-wide germline-enriched and sex-biased expression profiles in Caenorhabditis elegans
    Valerie Reinke
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA
    Development 131:311-23. 2004
    ..Comparison with data from large-scale RNAi screens demonstrates that genes expressed in the oogenic germline display visible phenotypes more frequently than expected...
  92. ncbi Regulated expression of the ubiquitin protein ligase, E3(Histone)/LASU1/Mule/ARF-BP1/HUWE1, during spermatogenesis
    Zhiqian Liu
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    Dev Dyn 236:2889-98. 2007
    ..Because we identified E3(Histone)/LASU1 from the testis, we explored its regulation during spermatogenesis. In the first wave of rat spermatogenesis, E3(Histone)/LASU1 mRNA and protein had peak expression at days 10 ..
  93. ncbi Absence of mouse REC8 cohesin promotes synapsis of sister chromatids in meiosis
    Huiling Xu
    Divisions of Radiation Oncology and Research, Peter MacCallum Cancer Centre, Melbourne, Victoria 8006, Australia
    Dev Cell 8:949-61. 2005
    ..Unexpectedly, Rec8 null mice are born in sub-Mendelian frequencies and fail to thrive. These findings illuminate hitherto unknown REC8 functions in chromosome dynamics during mammalian meiosis and possibly in somatic development...
  94. ncbi microRNAs in the testis: building up male fertility
    Marilena D Papaioannou
    Department of Genetic Medicine and Development, University of Geneva Medical School, CH 1211 Geneva 4, Switzerland
    J Androl 31:26-33. 2010
    b>Spermatogenesis is a strictly regulated process, at both the transcriptional and the posttranscriptional level, which allows continuous gamete production throughout adulthood...
  95. pmc Dicer is required for haploid male germ cell differentiation in mice
    Hanna M Korhonen
    Department of Physiology, Institute of Biomedicine, University of Turku, Turku, Finland
    PLoS ONE 6:e24821. 2011
    ..mouse male germ cells, and several miRNAs and endogenous siRNAs are expressed in the testis, suggesting that Dicer-dependent small RNAs are involved in the control of the precisely timed and highly organised process of spermatogenesis.
  96. pmc Alternative splicing of NURF301 generates distinct NURF chromatin remodeling complexes with altered modified histone binding specificities
    So Yeon Kwon
    Institute of Biomedical Research, University of Birmingham, Edgbaston, United Kingdom
    PLoS Genet 5:e1000574. 2009
    ..However, full-length NURF301 is required for spermatogenesis. Mutants that lack full-length NURF exhibit a spermatocyte arrest phenotype and fail to express a subset of ..
  97. ncbi Genomic and functional evolution of the Drosophila melanogaster sperm proteome
    Steve Dorus
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Nat Genet 38:1440-5. 2006
    ..The relevance of the DmSP to the study of mammalian sperm function and fertilization mechanisms is demonstrated by the identification of substantial homology between the DmSP and proteins of the mouse axoneme accessory structure...
  98. pmc Spermatogonial stem cell regulation and spermatogenesis
    Bart T Phillips
    Department of Obstetrics, Gynecology and Reproductive Sciences, Magee Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA, USA
    Philos Trans R Soc Lond B Biol Sci 365:1663-78. 2010
    ..be described, along with research using these tools to enhance our understanding of stem cell biology and spermatogenesis. Increased knowledge about the biology of SSCs improves our capacity to manipulate these cells for practical ..
  99. pmc Sertoli cell Dicer is essential for spermatogenesis in mice
    Marilena D Papaioannou
    Department of Genetic Medicine and Development, University of Geneva Medical School, 1, rue Michel Servet, 1211 Geneva 4, Switzerland
    Dev Biol 326:250-9. 2009
    b>Spermatogenesis requires intact, fully competent Sertoli cells. Here, we investigate the functions of Dicer, an RNaseIII endonuclease required for microRNA and small interfering RNA biogenesis, in mouse Sertoli cell function...
  100. ncbi Acquisition of the H19 methylation imprint occurs differentially on the parental alleles during spermatogenesis
    T L Davis
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Genomics 58:18-28. 1999
    ..The 2-kb region is methylated on the paternal allele throughout spermatogenesis, suggesting that methylation is acquired in this region prior to the resumption of mitosis in postnatal male ..
  101. pmc Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus
    Sunil Jayaramaiah Raja
    Philipps Universitat Marburg, Fachbereich Biologie, Entwicklungsbiologie, 35043 Marburg, Germany
    Mol Cell Biol 25:6165-77. 2005
    ..These data suggest the major features of chromatin condensation in Drosophila spermatogenesis correspond to those in mammals...

Research Grants81

  1. Cisplatin and Mechanisms of Long-term Male Infertility
    JOHN RICHBURG; Fiscal Year: 2007
    ..spermatogonia during chemotherapeutic treatment accounts for long-term infertility and/or delayed recovery of spermatogenesis. We recently showed, in mice, that a large proportion of stem spermatogonia survive clinic-like repeated ..
  2. Collagen X and heparan sulfate proteoglycan in the hematopoietic stem cell niche.
    ELIZABETH G SWEENEY; Fiscal Year: 2012
    ..transcriptional regulation of differentiation, growth hormones, respiration, cell cycle, cell death, spermatogenesis, obesity, stress, immunology, eye development, and dinosaur paleobiology...
  3. Interdisciplinary Research Training in Reproduction for Indian Fellows
    John C Herr; Fiscal Year: 2010
    ..physiology of ion channels, neuroendocrine control of mechanisms in reproduction, molecular control of spermatogenesis and oogenesis, autoimmunity to sperm antigens;sperm maturation in the epididymis, and circadian rhythms...
  4. Novel genomic effects of Y-linked polymorphisms
    Daniel L Hartl; Fiscal Year: 2012
    ..experiments will also identify a set of YRV-affected candidate genes associated with thermal tolerance of spermatogenesis, a key biological factor in limiting the ecological range of many insects including the mosquitoes that ..
  5. Analysis of the role of the VRK1 protein kinase in mammalian fertility
    Paula Traktman; Fiscal Year: 2011
    ..However, the signaling cascades critical to both spermatogenesis and oogenesis are still poorly understood...
  6. Regulation of insulins by the Rhox5 homeobox gene supports spermatogenesis
    James Maclean; Fiscal Year: 2009
    ..Rhox) genes are androgen regulated and most are expressed in Sertoli cells (the nurse cells that support spermatogenesis), suggesting that they regulate the expression of somatic-cell gene products crucial for germ-cell ..
  7. MECHANISMS OF FGF RECEPTOR REGULATION AND SIGNALING
    Moosa Mohammadi; Fiscal Year: 2013
    ..The paracrine FGF1, FGF4, FGF7, FGF8, and FGF9 subfamilies play essential roles in spermatogenesis, mesoderm induction, somitogenesis, organogenesis, and pattern formation, whereas the FGF19 subfamily acts in ..
  8. THE EFFECT OF DIOXINS ON GLUCOSE HOMEOSTASIS IN MURINE TESTES
    KENAN RIFAT OMURTAG; Fiscal Year: 2013
    ..if dioxin exposure affects gene expression in the germ cells and perhaps stem cells at different stages of spermatogenesis. These changes may not manifest until much later relative to exposure and thus may explain the paternal ..
  9. The biology of blood-testis barrier dynamics
    C Yan Cheng; Fiscal Year: 2013
    ..of this proposal is to understand the biology and regulation of blood-testis barrier (BTB) dynamics during spermatogenesis using the adult rat testis as a study model...
  10. MRNA PROCESSING IN REPRODUCTION AND FERTILIZATION
    Clinton C MacDonald; Fiscal Year: 2013
    ..These differences are essential for the correct expression of key genes during spermatogenesis, for example altering the protein forms of transcription factors or altering translational efficiency...
  11. Model of the Human Testis for Reproductive Toxicology
    PAUL JACOB TUREK; Fiscal Year: 2011
    ..The overall goal of the research is to develop a functional in vitro human cell-based testis model of spermatogenesis as a clinically relevant product for reproductive toxicology...
  12. The role of satellite rasiRNA in the Segregation Distorter system of Drosophila
    AMANDA MARIE LARRACUENTE; Fiscal Year: 2013
    ..Many features of spermatogenesis in eukaryotes cannot be explained without invoking a species history of genetic conflict (e.g...
  13. AAA ATPases linking mitochondria with microtubule processing in flies and yeast
    Karen G Hales; Fiscal Year: 2012
    ..Drosophila melanogaster spermatogenesis is an ideal model system for genetic dissection of conserved mechanisms of mitochondrial morphogenesis, since ..
  14. PTIP as an epigenetic regulator in male meiosis
    Guo Zhang Zhu; Fiscal Year: 2013
    ..mechanisms, including DNA methylation, histone modification and non-coding RNAs, play significant roles in spermatogenesis. Despite recent progress, our comprehension of the dynamic epigenome in developing male germ cells remains ..
  15. Environmental Endocrine Disruptors and Human Sperm Chromosomal Abnormalities
    Melissa Perry; Fiscal Year: 2012
    ..There is emerging evidence that environmental chemicals can adversely affect spermatogenesis and the occurrence of chromosomal aberrations, possibly through mechanisms of endocrine hormone modulation...
  16. The evolutionary and molecular mechanisms underlying sperm performance in an emer
    Heidi S Fisher; Fiscal Year: 2013
    ..approached combined with gene expression studies of the testicular tissue that represent different stages of spermatogenesis. During the independent phase of this project the focus will be on exploring sperm aggregation behavior to ..
  17. Role of nuclear receptors in origin and differentiation of spermatozoa
    ADAM MICHAEL REITZEL; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Spermatogenesis is a complex developmental process where undifferentiated spermatogonia are differentiated into mature spermatozoa that are then able to fertilize oocytes...
  18. Regulation and Function of the Rhox5 Homeobox Gene
    MILES FROME WILKINSON; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Spermatogenesis is an androgen-dependent process requiring intimate contact between the cells that directly respond to the androgenic signal (Sertoli cells) and the cells that ultimately become sperm ..
  19. The Role of a New Cleavage and Polyadenylation Specificity Factor (CPSF73-II)
    Qingshun Q Li; Fiscal Year: 2010
    ..in the process of 3'-end formation have been linked to viral infection, tumor development, and spermatogenesis in human and animals...
  20. Catenins: A role in spertmatogenesis and sperm maturation
    MANJEET KUMAR RAO; Fiscal Year: 2010
    ..these studies could lead to new insights for therapeutically compromising germ cell movement in the seminiferous epithelium, thereby disrupting spermatogenesis, as a novel approach to reversible male contraception.
  21. Human Exposure to Bisphenol A, Phthalates and Fertility, Pregnancy Outcomes
    Russ B Hauser; Fiscal Year: 2013
    ..BPA has been shown in experimental studies to lead to oocyte aneuploidy, pregnancy loss and adversely affect spermatogenesis. The proposed competing continuation is designed to determine the developmental and reproductive toxicity of ..
  22. TRANSPLANTATION OF TESTIS STEM CELLS IN LARGE ANIMALS
    Ina Dobrinski; Fiscal Year: 2013
    ..It allows reconstitution of spermatogenesis from isolated cells in an in vivo culture system, a process that is currently not possible in vitro...
  23. The MRAD9 Radioresistance Gene
    Howard B Lieberman; Fiscal Year: 2013
    ..of Rad9, such as roles in maintaining genomic stability and homologous recombination, which are critical for spermatogenesis, we investigated whether Rad9 functions in this process...
  24. The XXII nd North American Testis Workshop
    WILLIAM WALLACE WRIGHT; Fiscal Year: 2013
    ..Regents Professor, Washington State University, will present a genome-wide perspective on the regulation of spermatogenesis. Dr...
  25. Hormonal and Paracrine Regulation of Spermatogenesis
    Barry R Zirkin; Fiscal Year: 2010
    The focus of this application is on the hormonal and paracrine regulation of spermatogenesis in humans and rodents...
  26. Intratesticular Sex Steroids and Spermatogenesis in Man
    MARA YVONNE ROTH; Fiscal Year: 2013
    ..prior research by working to define the intratesticular hormonal characteristics required for spermatogenesis, which will help target the challenge of developing a more effective male hormonal contraceptive regimen...
  27. Biology of desmosome-gap junctions in the testis
    Dolores D Mruk; Fiscal Year: 2010
    ..has not been a single report addressing the biology of desmosome-gap junctions and their significance in spermatogenesis. This application aims to fill this gap by investigating desmosome-gap junction dynamics in the mammalian ..
  28. Developmental Regulation of the Cell Cycle in Drosophila
    LAURA ANNE LEE; Fiscal Year: 2013
    ..b>Spermatogenesis utilizes mitotic and meiotic cell cycles coordinated with growth and differentiation programs to generate ..
  29. Structural Targeting Testis Specific Kinases (TSSK) 1 &2 for Male Contraception
    John C Herr; Fiscal Year: 2013
    ..These key findings substantiate an indispensable role for TSSK 1 &2 and the TSSK 1 &2 pathway in spermatogenesis, providing the primary evidence for this study's central hypothesis: that administration of a small ..
  30. Role of Cyclin A's in mammalian gametogenesis
    Debra J Wolgemuth; Fiscal Year: 2013
    ..We showed that cyclin A1 is testis-specific and by targeted mutagenesis, that it is essential for spermatogenesis--spermatocytes arrest at the diplotene to metaphase 1 transition...
  31. Regulation of Spermatocyte Transcription by Testis TAFS
    Margaret T Fuller; Fiscal Year: 2013
    ..Using the dramatic cellular differentiation program of spermatogenesis as a model stem cell lineage, we seek to understand the unique, cell type specific transcription program ..
  32. BDADs for Male Contraception
    John K Amory; Fiscal Year: 2013
    ..Bis-dichloroacetyl-diamines (BDADs) such as WIN 18,446 was shown to safely, completely and reversibly inhibit spermatogenesis in men...
  33. MOLECULAR BASIS OF MAMMALIAN SPERM MOTILITY
    Jerome F Strauss; Fiscal Year: 2010
    ..phosphorylation regulates PF20s function;and 4) that PF20 mutations cause abnormalities in human spermatogenesis as well as primary ciliary dyskinesia...
  34. microRNA regulation of spermatogonial stem cell self-renewal and differentiation
    RALPH LAWRENCE BRINSTER; Fiscal Year: 2013
    ..is an adult stem cell capable of both self-renewal and differentiation, thereby playing a critical role in spermatogenesis. Understanding the processes that govern SSC self-renewal and differentiation will provide essential ..
  35. Lectin Resistant Tumor Cells And Functional Glycomics
    PAMELA M STANLEY; Fiscal Year: 2013
    ..specifically inhibit MGAT1, thereby allowing spermatocytes to associate appropriately with Sertoli cells and spermatogenesis to proceed...
  36. The epigenetic basis of cytoplasmic incompatibility
    KRISTIN KALIE JERNIGAN; Fiscal Year: 2012
    ..three key points: (i) First, since Wolbachia are stripped from the sperm in the cytoplasmic waste bag during spermatogenesis, CI must involve a "modification" of the sperm in the testes of a Wolbachia-infected male...
  37. Cyclic AMP Action During Sperm Function
    George L Gerton; Fiscal Year: 2012
    ..It is critical for mammalian spermatogenesis, maturation of sperm in the epididymis, and capacitation, the post-epididymal maturational process that sperm ..
  38. Evolutionary and Functional Genetics of Male Reproduction using Wild Mice as a Mo
    Matthew D Dean; Fiscal Year: 2013
    ..We have four specific aims: (1) Genome Evolution - to sequence 400 seminal fluid genes and 400 spermatogenesis genes from wild caught individuals sampled from eight species of Mus, including population level samples from ..
  39. Characterization of male germline stem cells in a rhesus model of infertility
    Kyle E Orwig; Fiscal Year: 2012
    Abstract Spermatogonial stem cells (SSCs) are at the foundation of mammalian spermatogenesis. In rodents, the SSC pool is comprised of type Asingle (As) spermatogonia that balance self-renewing and differentiating divisions to exactly ..
  40. Evolution of Sexually Dimorphic Germ Cells in Volvox carteri
    James Umen; Fiscal Year: 2013
    ..We will use trans-species complementation to determine the key changes that allowed vcMid to become a spermatogenesis factor in Volvox. 2. Remodeling of gene regulatory network outputs for vcMid...
  41. Biochemistry of lysine crotonylation pathway
    Yingming Zhao; Fiscal Year: 2013
    ..then identify substrates of Kcr and study their dynamics in response to Kcr- regulatory enzymes and during spermatogenesis, by a quantitative proteomics approach...
  42. A Novel Testicular Germ Cell Maturation Model for Fertility Preservation in Pre-p
    Kirk C Lo; Fiscal Year: 2012
    ..The second part of our project is to induce spermatogenesis in our human testis xenotransplantation model with gonadotropin stimulation...
  43. FUNCTION AND REGULATION OF GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 2
    MARIA R GONZALEZ BARO; Fiscal Year: 2012
    ..Transcriptional regulation of GPAT2 will be determined in cells exposed to spermatogenesis-associated hormones, and by expressing the GPAT2 promoter region in a reporter-gene construct...
  44. Function of the Bromodomain Protein Brdt in Spermatogenesis
    Debra J Wolgemuth; Fiscal Year: 2013
    ..a phenotype distinct from that of the Brdt[unreadable]BD1 mutants: Brdt-/- male mice are also sterile but spermatogenesis is arrested in late meiotic prophase spermatocytes and spermatids do not form...
  45. Regulation of spermatogenesis by sumoylation
    Margarita Vigodner; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Successful progression through spermatogenesis is crucial for normal gamete formation and for transferring the genetic information to the next generation...
  46. Cyclic Nucleotide-Activated Calcium Channels in Sperm
    Dejian Ren; Fiscal Year: 2010
    ..Aim 2 will uncover the molecular mechanism underlying the CatSper1 function regulation during spermatogenesis and sperm maturation. Four hypotheses will be tested: a. the CatSper1 protein expression is regulated;b...
  47. Do we need Y chromosome for successful reproduction?
    Monika A Ward; Fiscal Year: 2013
    ..In Preliminary Data we show that males with a Y complement limited to Sry and Eif2s3y have spermatogenesis arrest and do not produce mature sperm...
  48. Transcriptome-wide Polyadenylation Site Use in Male Germ Cell Transcripts
    BECKY SARTINI; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Spermatogenesis requires extensive control of gene expression to coordinate the unique cell proliferation and differentiation events...
  49. Retinoid receptor antagonists as novel male contraceptives
    Debra J Wolgemuth; Fiscal Year: 2013
    The overarching goal of these studies is to understand the role of retinoid signaling in the regulation of spermatogenesis and to exploit the inhibition of its essential function as a new and novel approach to male contraception...
  50. Defining the Mammalian Intercellular Bridge Interactome
    Martin M Matzuk; Fiscal Year: 2010
    ..Thus, intercellular bridges are critical for mammalian spermatogenesis but not oogenesis and TEX14 is the first vertebrate protein that is essential for the intercellular bridge...
  51. Mechanisms of Circadian Rhythmicity in CLOCK-Deficient Mice
    DAVID RAYMOND WEAVER; Fiscal Year: 2013
    ..of these genes, ranging from potential effects on reproductive endocrinology and behavior to defects in spermatogenesis (for infertility), and multiple potential cell types of the musculo-skeletal system (for arthropathy)...
  52. Reversal of Spermatogonial Arrest in Mice
    Gunapala Shetty; Fiscal Year: 2012
    ..will provide insight into the molecular mechanisms underlying aspects of temperature and androgen effects on spermatogenesis and reveal a direct mechanistic link between these two modulators...
  53. Animal Model to Study Retinoic Acid Function in Postnatal and Adult Tissues
    GREGG L DUESTER; Fiscal Year: 2012
    ..suggests that RA may also control many adult functions including hippocampal neurogenesis, immune function, spermatogenesis, stem cell function, skin and hair follicle regeneration, and prevention of cancer...
  54. Penn Center for Study of Epigenetics in Reproduction
    Marisa S Bartolomei; Fiscal Year: 2013
    ..Project III (Berger) will investigate histone modifications during mouse spermatogenesis and determine their conservation in normal human sperm and disruption in abnormal human sperm and mouse ..
  55. Epigenetic Reprogramming in Germline by Phthalates
    Kwan Hee Kim; Fiscal Year: 2013
    ..characterized by postnatal anomalies such as increased crytorchidism, shortened anogenital distance, aberrant spermatogenesis, and testicular cancer, stemming from impaired fetal testicular development...
  56. Nongenomic Androgen Signaling in Sertolli Cells
    William H Walker; Fiscal Year: 2010
    ..of this project remains to determine the molecular and cellular mechanisms by which testosterone regulates spermatogenesis. Testosterone is essential for fertility and targets Sertoli cells in the mammalian testis through the ..
  57. Transcriptional regulatory networks in spermatogonial stem cells
    BRIAN PETER HERMANN; Fiscal Year: 2013
    Project Summary/Abstract Spermatogonial stem cells (SSCs) are at the foundation of mammalian spermatogenesis, maintaining sperm production throughout adult life. The molecular mechanisms that control the critical self-renewal vs...
  58. AMERICAN SOCIETY OF ANDROLOGY ANNUAL MEETING
    Dolores J Lamb; Fiscal Year: 2010
    ..g., cell and molecular biology of spermatogenesis and fertilization, reproductive organ and tract development, mechanisms of endocrine action, reproductive ..
  59. Developmental Control of the Cell Cycle in Male Meiosis
    Margaret T Fuller; Fiscal Year: 2010
    ..Our findings will illuminate the mode of action and regulation of the conserved RNA binding protein Boule and suggest mechanisms to test for similar function during mammalian spermatogenesis.
  60. Regulation of Spermatogenesis by X-linked miRNAs
    Wei Yan; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Meiotic sex chromosome inactivation (MSCI) during spermatogenesis is characterized by transcriptional silencing of genes on both the X and Y chromosomes in mid to late pachytene spermatocytes...
  61. Protein Phosphatase Action in Mammalian Spermatogenesis and Sperm Function
    Srinivasan Vijayaraghavan; Fiscal Year: 2012
    ..Statement Alternatively spliced protein phosphatase PP1?2 isoform of PP1 ? gene plays an essential role in spermatogenesis and mammalian reproduction...
  62. Osteocalcin: the hormonal link between bone and reproduction
    Gerard Karsenty; Fiscal Year: 2013
    ..and pharmacologically whether it is the carboxylated or the uncarboxylated form of osteocalcin that regulates spermatogenesis - To demonstrate genetically that it is through its osteoblast expression that osteocalcin favors sperm ..
  63. Minimum Y gene complement necessary for successful ART
    MONIKA WARD; Fiscal Year: 2009
    ..of this application is that it will advance the understanding of the role of Y chromosome encoded genes in spermatogenesis and sperm function;it will also provide valuable information for those using ART to treat human infertility ..
  64. The Sertoli Cell Product, Doppel, and Male Fertility
    William Wright; Fiscal Year: 2009
    b>Spermatogenesis occurs in a unique environment that is created by the somatic Sertoli cells...
  65. OPTIMIZING SPERMATOGONIAL TRANSPLANTATION FOR FERTILITY PRESERVATION
    Gunapala Shetty; Fiscal Year: 2010
    Exposure of men to gonadotoxic cancer therapeutic agents can severely impair spermatogenesis. Many of the radiotherapeutic and chemotherapeutic regimens may result in complete killing of the stem spermatogonia without a chance for ..
  66. Secretory Vesicle Function During Spermatogenesis & Fertilization in C. elegans
    STEVEN W L apos HERNAULT; Fiscal Year: 2010
    ..mechanisms that regulate V-ATPase activity in sperm secretory vesicles are not understood, mostly because a spermatogenesis system suitable for its analysis had not been identified...
  67. Aromatase Inhibitor in Males with Adrenal Hyperplasia
    Kyriakie Sarafoglou; Fiscal Year: 2006
    ..by applicant): Our objective is to treat males who have congenital adrenal hyperplasia (CAH) and decreased spermatogenesis with an aromatase inhibitor in order to investigate its effects on hormonal parameters and spermatogenesis...
  68. CONSEQUENCES OF MALE LH RECEPTOR GENE KNOCKOUT
    Zhenmin Lei; Fiscal Year: 2002
    ..The external and internal genitalia of homozygous males were grossly underdeveloped and spermatogenesis was arrested at the spermatocytes...
  69. MEIOTIC CELL CYCLE REGULATION IN REPRODUCTION
    Stuart Ravnik; Fiscal Year: 2002
    ..the cyclins and Cdks during meiosis, especially with regard to the meiotic divisions that take place during spermatogenesis. The applicant's previous research has shown that a novel cyclin, Cyclin Al (CycAl) is expressed during mouse ..
  70. SPERMATOGENESIS: SERTOLI-GERM CELL INTERACTION
    ANNA STEINBERGER; Fiscal Year: 1991
    The broad objective is to clarify the regulation of mammalian spermatogenesis, and the role played by Sertoli cells (Sc) in the endocrine and paracrine aspects of this prccess...
  71. REGULATION AND FUNCTION OF HEAT SHOCK FACTORS IN TESTIS
    KEVIN SARGE; Fiscal Year: 1999
    b>Spermatogenesis is the process by which immature male germ cells, through a complex series of events involving mitosis, meiosis, and differentiation of distinct spermatogenic cell types, are transformed into mature spermatozoa capable of ..
  72. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..K9 and Sak 57 will prove to be of profound developmental significance in spermatogenesis. K9 is of particular interest because its gene expression occurs in two sites only: the footpad epidermis and ..
  73. Regulation of Gene Expression During Spermatogenesis
    Peter Petrusz; Fiscal Year: 2004
    Androgens are indispensable for normal spermatogenesis; however, the mechanism by which androgens exert their effect on this complex process is poorly understood...
  74. ALTERNATIVE MRNA PROCESSING DURING SPERMATOGENESIS
    PETER CHALLONER; Fiscal Year: 1992
    ..molecular basis of the alternative pre-mRNA processing reactions that occur during the post-meiotic stages of spermatogenesis. In spermatids, both polyadenylation and splicing at alternative sites produce a variety of unique MRNA ..
  75. MECHANISMS OF TOXICITY IN TESTES
    Michael Welsh; Fiscal Year: 1999
    ..this highly significant problem, a better understanding is needed of mechanisms by which toxicants affect spermatogenesis in mammals...
  76. ANALYSIS OF THE ROLE OF GCNF IN SPERMATOGENESIS
    Austin Cooney; Fiscal Year: 1999
    ..Thus, understanding male idiopathic infertility requires an understanding of spermatogenesis at the molecular level...
  77. Pem Homeobox Gene Function During Spermatogenesis
    J Suzanne Lindsey; Fiscal Year: 2004
    ..Communication between Sertoli cells of the testis and germ cells is essential for spermatogenesis. Testosterone regulates spermatogenesis through binding Sertoli cell androgen receptors and resultant gene ..
  78. 1991 TESTIS WORKSHOP
    Bernard Robaire; Fiscal Year: 1991
    ..developments in our understanding of the underlying genomic expression and control that takes place during spermatogenesis and sperm maturation and fertilization have led the Program Committee to focus the 1991 Workshop on selective ..
  79. MALE INFERTILITY AND ITS BIOLOGICAL BASIS
    Barry Zirkin; Fiscal Year: 2002
    ..The results of Projects I and II should elucidate common principles involved in regulating spermatogenesis in rats and men...
  80. HU-IME1 CHARACTERIZATION AND MALE INFERTILITY
    Wael Salameh; Fiscal Year: 1999
    ..Meiosis occurs only in the gonads. In the mammalian male, spermatogenesis is an ongoing process in which stem cell proliferation, meiosis, and cellular differentiation occur ..
  81. Regulation of spermatogonial stem cells by vitamin A
    Derek McLean; Fiscal Year: 2007
    ..problems are fully or partially attributed to male-related factors which include: low sperm count, abnormal spermatogenesis, and reduced androgen production...