Genomes and Genes
Summary: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Publications379 found, 100 shown here
- One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsK A Datsenko
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
Proc Natl Acad Sci U S A 97:6640-5. 2000..with 36- to 50-nt extensions that are homologous to regions adjacent to the gene to be inactivated and template plasmids carrying antibiotic resistance genes that are flanked by FRT (FLP recognition target) sites...
- Enzymatic assembly of DNA molecules up to several hundred kilobasesDaniel G Gibson
The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
Nat Methods 6:343-5. 2009..This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool...
- Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiaeM S Longtine
Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
Yeast 14:953-61. 1998..We describe here a new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications...
- Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological studyKarthikeyan K Kumarasamy
Department of Microbiology, Dr ALM PG IBMS, University of Madras, Chennai, India
Lancet Infect Dis 10:597-602. 2010..We investigated the prevalence of NDM-1, in multidrug-resistant Enterobacteriaceae in India, Pakistan, and the UK...
- An archaeal immune system can detect multiple protospacer adjacent motifs (PAMs) to target invader DNASusan Fischer
Department of Biology II, Ulm University, 89069 Ulm, Germany
J Biol Chem 287:33351-63. 2012..only a few species have been investigated to elucidate the precise mechanisms for the defense of viruses or plasmids. Approximately 90% of all sequenced archaea encode CRISPR/Cas systems, but their molecular details have so far ..
- Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing siteAsma Hatoum-Aslan
Laboratory of Bacteriology, The Rockefeller University, New York, NY 10065, USA
Proc Natl Acad Sci U S A 108:21218-22. 2011..We also show that maturation is mediated by specific cas genes distinct from those genes involved in primary processing, showing that this event is directed by CRISPR/Cas loci...
- The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNAJosiane E Garneau
Departement de Biochimie, de microbiologie et de bio informatique, Faculte des sciences et de genie, Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Felix d Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec City, Quebec G1V 0A6, Canada
Nature 468:67-71. 2010..and Archaea have developed several defence strategies against foreign nucleic acids such as viral genomes and plasmids. Among them, clustered regularly interspaced short palindromic repeats (CRISPR) loci together with cas (CRISPR-..
- Prokaryotic toxin-antitoxin stress response lociKenn Gerdes
Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
Nat Rev Microbiol 3:371-82. 2005Although toxin-antitoxin gene cassettes were first found in plasmids, recent database mining has shown that these loci are abundant in free-living prokaryotes, including many pathogenic bacteria...
- Dissemination of clonally related Escherichia coli strains expressing extended-spectrum beta-lactamase CTX-M-15Teresa M Coque
Hospital Universitario Ramon y Cajal, Madrid, Spain
Emerg Infect Dis 14:195-200. 2008We analyzed 43 CTX-M-15-producing Escherichia coli isolates and 6 plasmids encoding the blaCTX-M-15 gene from Canada, India, Kuwait, France, Switzerland, Portugal, and Spain. Most isolates belonged to phylogroups B2 (50%) and D (25%)...
- DNA assembler, an in vivo genetic method for rapid construction of biochemical pathwaysZengyi Shao
Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
Nucleic Acids Res 37:e16. 2009....
- Identification of plasmids by PCR-based replicon typingAlessandra Carattoli
Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
J Microbiol Methods 63:219-28. 2005The epidemiological importance of tracing plasmids conferring drug resistance prompted us to develop a PCR method based on replicons (inc/rep PCR) of the major plasmid incompatibility groups among Enterobacteriaceae...
- Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinantP P Cherepanov
Universitat Oldenburg, Germany
Gene 158:9-14. 1995..This system was applied in the construction of an E. coli endA deletion mutation which can be transduced by P1 to the genetic background of interest using TcR as a marker. The transductant can then be freed of the TcR if required...
- Escherichia coli O25b-ST131: a pandemic, multiresistant, community-associated strainBenjamin A Rogers
The University of Queensland, UQ Centre for Clinical Research, Herston, 4006, Brisbane, Australia
J Antimicrob Chemother 66:1-14. 2011..Phenotypic detection of the ST131 clone is not possible and DNA-based techniques, including MLST and PCR, are described...
- Selective and hyperactive uptake of foreign DNA by adaptive immune systems of an archaeon via two distinct mechanismsSusanne Erdmann
Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
Mol Microbiol 85:1044-56. 2012..The results underline the complex dynamics of CRISPR-based immune systems within a population infected with genetic elements...
- The diversity of conjugative relaxases and its application in plasmid classificationMaria Pilar Garcillán-Barcia
Departamento de Biología Molecular e Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria CSIC IDICAN, Santander, Spain
FEMS Microbiol Rev 33:657-87. 2009..Bacterial conjugation is an efficient and sophisticated mechanism of DNA transfer among bacteria. While mobilizable plasmids only encode a minimal MOB machinery that allows them to be transported by other plasmids, conjugative plasmids ..
- Extended-spectrum beta-lactamases: a clinical updateDavid L Paterson
Infectious Disease Division, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
Clin Microbiol Rev 18:657-86. 2005..The presence of ESBLs carries tremendous clinical significance. The ESBLs are frequently plasmid encoded. Plasmids responsible for ESBL production frequently carry genes encoding resistance to other drug classes (for example, ..
- CRISPR/Cas, the immune system of bacteria and archaeaPhilippe Horvath
Danisco France SAS, BP10, F 86220 Dangé Saint Romain, France
Science 327:167-70. 2010..palindromic repeats (CRISPR) form peculiar genetic loci, which provide acquired immunity against viruses and plasmids by targeting nucleic acid in a sequence-specific manner...
- Genome stability of Lyme disease spirochetes: comparative genomics of Borrelia burgdorferi plasmidsSherwood R Casjens
Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, United States of America
PLoS ONE 7:e33280. 2012..Towards this end we present a comparative analysis of the nucleotide sequences of the numerous plasmids of B. burgdorferi isolates B31, N40, JD1 and 297...
- Recombineering: a homologous recombination-based method of genetic engineeringShyam K Sharan
Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
Nat Protoc 4:206-23. 2009..coli genome can be subcloned by gene retrieval using recombineering. Most of these constructs can be made within about 1 week's time...
- Emergent properties of reduced-genome Escherichia coliGyörgy Pósfai
Institute of Biochemistry, Biological Research Center, H 6726 Szeged, Hungary
Science 312:1044-6. 2006..beneficial properties: high electroporation efficiency and accurate propagation of recombinant genes and plasmids that were unstable in other strains...
- Recombinant protein expression in Escherichia coliF Baneyx
Department of Chemical Engineering University of Washington Box 351750, Seattle, WA 98195, USA
Curr Opin Biotechnol 10:411-21. 1999..coli, together with serendipitous discoveries and the availability of improved genetic tools are making this bacterium more valuable than ever for the expression of complex eukaryotic proteins...
- The CTX-M beta-lactamase pandemicRafael Canton
Servicio de Microbiologia, Hospital Ramon y Cajal, 28034 Madrid, Spain
Curr Opin Microbiol 9:466-75. 2006..These structures have been located in narrow and broad host-range plasmids belonging to the same incompatibility groups as those of early antibiotic resistance plasmids...
- Dynamic properties of the Sulfolobus CRISPR/Cas and CRISPR/Cmr systems when challenged with vector-borne viral and plasmid genes and protospacersSoley Gudbergsdottir
Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen, Denmark
Mol Microbiol 79:35-49. 2011..Moreover, the relatively high incidence of single-spacer deletions observed for S. islandicus suggests that an additional more directed mechanism operates in this organism...
- GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modulesAlejandro Sarrion-Perdigones
Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas, Universidad Politecnica de Valencia, Valencia, Spain
PLoS ONE 6:e21622. 2011..The GB system consists of a set of four destination plasmids (pDGBs) designed to incorporate multipartite assemblies made of standard DNA parts and to combine them binarily ..
- Plasmid-mediated quinolone resistance: a multifaceted threatJacob Strahilevitz
Department of Clinical Microbiology and Infectious Diseases, Hadassah Ein Kerem, Jerusalem 91120, Israel
Clin Microbiol Rev 22:664-89. 2009..Of concern, these genes are not yet being taken into account in resistance screening by clinical microbiology laboratories...
- A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettesCarsten Janke
CRBM, CNRS FRE2593, 1919 Route de Mende, F 34293 Montpellier cedex 5, France
Yeast 21:947-62. 2004..This new toolbox should help to speed up the analysis of gene function in yeast, on the level of single genes, as well as in systematic approaches...
- Broad-host-range plasmids from agricultural soils have IncP-1 backbones with diverse accessory genesDiya Sen
Department of Biological Sciences, Institute for Bioinformatics and Evolutionary Studies IBEST, University of Idaho, Moscow, ID 83844 3051, USA
Appl Environ Microbiol 77:7975-83. 2011Broad-host-range plasmids are known to spread genes between distinct phylogenetic groups of bacteria. These genes often code for resistances to antibiotics and heavy metals or degradation of pollutants...
- Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coliYoshitoshi Ogura
Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, 5200 Kiyotake, Miyazaki 889 1692, Japan
Proc Natl Acad Sci U S A 106:17939-44. 2009..We found many lambdoid phages, IEs, and virulence plasmids that carry the same or similar virulence genes but have distinct evolutionary histories, indicating that ..
- Stochastic gene expression in a single cellMichael B Elowitz
Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
Science 297:1183-6. 2002..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...
- The ClosTron: a universal gene knock-out system for the genus ClostridiumJohn T Heap
Institute of Infection, Immunity and Inflammation, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
J Microbiol Methods 70:452-64. 2007..The procedure is highly efficient and reproducible, and should revolutionize functional genomic studies in clostridia...
- A modular system for Clostridium shuttle plasmidsJohn T Heap
BBSRC Sustainable BioEnergy Centre, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK
J Microbiol Methods 78:79-85. 2009..The transfer and maintenance of recombinant, replicative plasmids into various species of Clostridium has been reported, and several elements suitable as shuttle plasmid ..
- Prions affect the appearance of other prions: the story of [PIN(+)]I L Derkatch
Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607, USA
Cell 106:171-82. 2001..Importantly, the de novo appearance of Rnq1::GFP prion aggregates also requires the presence of other prions, suggesting the existence of a general mechanism by which the appearance of prions is enhanced by heterologous prion aggregates...
- Analysis of the resistome of a multidrug-resistant NDM-1-producing Escherichia coli strain by high-throughput genome sequencingLaurent Poirel
Service de Bacteriologie Virologie, Hopital de Bicetre, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre cedex, France
Antimicrob Agents Chemother 55:4224-9. 2011..These resistance genes were located on three additional plasmids, of 160 kb (IncA/C), 130 kb (IncF), and 110 kb (IncI1)...
- Localization of ASH1 mRNA particles in living yeastE Bertrand
Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Mol Cell 2:437-45. 1998..Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization...
- An efficient recombination system for chromosome engineering in Escherichia coliD Yu
Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
Proc Natl Acad Sci U S A 97:5978-83. 2000..Gene disruptions and modifications of both the bacterial chromosome and bacterial plasmids are possible...
- A synthetic oscillatory network of transcriptional regulatorsM B Elowitz
Department of Molecular Biology and Physics, Princeton University, New Jersey 08544, USA
Nature 403:335-8. 2000..Such 'rational network design may lead both to the engineering of new cellular behaviours and to an improved understanding of naturally occurring networks...
- A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeastU Gueldener
Heinrich Heine Universitat, Institut fur Mikrobiologie, Universitatsstrasse 1, Geb 26 12 01 64, 40225 Dusseldorf, Germany
Nucleic Acids Res 30:e23. 2002..The set of disruption cassettes and Cre expression plasmids described here represents a significant further development of the marker rescue system, which is ideally suited ..
- Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiersSebastian Bultmann
Department of Biology, Center for Integrated Protein Science Munich CIPSM, Ludwig Maximilians University Munich, Germany
Nucleic Acids Res 40:5368-77. 2012..These results show that dTALEs in combination with chemical manipulation of epigenetic modifiers facilitate targeted transcriptional activation of epigenetically silenced target genes...
- Extended-spectrum β-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factorsJan A J W Kluytmans
Laboratory for Medical Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands
Clin Infect Dis 56:478-87. 2013..Now, we have extended the analysis with plasmid replicons, virulence factors, and highly discriminatory genomic profiling methods...
- Replicon sequence typing of IncF plasmids carrying virulence and resistance determinantsLaura Villa
Department of Infectious, Parasitic and Immuno Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
J Antimicrob Chemother 65:2518-29. 2010IncF plasmids are frequently encountered in clinical enterobacterial strains associated with the dissemination of relevant antimicrobial resistance and virulence genes...
- DNA vaccines: an historical perspective and view to the futureMargaret A Liu
ProTherImmune, Lafayette, CA, USA
Immunol Rev 239:62-84. 2011....
- Plasmids foster diversification and adaptation of bacterial populations in soilHolger Heuer
Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn Institut Federal Research Centre for Cultivated Plants, Braunschweig, Germany
FEMS Microbiol Rev 36:1083-104. 2012It is increasingly being recognized that the transfer of conjugative plasmids across species boundaries plays a vital role in the adaptability of bacterial populations in soil...
- Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000Helene Feil
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 102:11064-9. 2005..5 mbp in size, composed of a circular chromosome and two plasmids. Although a high degree of similarity exists between the two sequenced Pseudomonads, 976 protein-encoding genes ..
- Genetic features of blaNDM-1-positive EnterobacteriaceaeLaurent Poirel
Service de Bacteriologie Virologie, Hopital de Bicetre, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre cedex, France
Antimicrob Agents Chemother 55:5403-7. 2011..The bla(NDM-1) plasmids coharbored a variety of resistance determinants, including β-lactamase genes, quinolone resistance genes, and ..
- CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulationDevaki Bhaya
Carnegie Institution for Science, Department of Plant Biology, Stanford, California 94305, USA
Annu Rev Genet 45:273-97. 2011..Exciting breakthroughs in understanding the mechanisms of the CRISPR-Cas system and its potential for biotechnological applications and understanding evolutionary dynamics are discussed...
- Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteriaM Herrero
GBF National Research Centre for Biotechnology, Braunschweig, Federal Republic of Germany
J Bacteriol 172:6557-67. 1990..of foreign genes into the chromosomes of gram-negative eubacteria was developed by combining in two sets of plasmids (i) the transposition features of Tn10 and Tn5; (ii) the resistances to the herbicide bialaphos, to mercuric ..
- The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patientsMatthew T G Holden
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge CB10 1SA, United Kingdom
J Bacteriol 191:261-77. 2009..The J2315 genome contains evidence that its unique and highly adapted genetic content has played a significant role in its success as an epidemic CF pathogen...
- New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteriaMaryvonne Arnaud
Unité de Biologie des Bactéries Pathogènes à Gram Positif, Institut Pasteur, Paris, France
Appl Environ Microbiol 70:6887-91. 2004..The plasmid was used in Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus to efficiently construct mutants with or without an associated antibiotic resistance gene...
- Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune systemTomas Sinkunas
Department of Protein DNA Interactions, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania
EMBO J 30:1335-42. 2011..foreign nucleic acids by utilizing small guide crRNAs (CRISPR RNAs) to interfere with invading viruses and plasmids. In Escherichia coli, Cas3 is essential for crRNA-guided interference with virus proliferation...
- Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmidsAnton V Bryksin
Department of Biochemistry, Center for Fundamental and Applied Molecular Evolution, Emory University, Atlanta, Georgia 30322, USA
Biotechniques 48:463-5. 2010..Phusion DNA polymerase was used for the amplification and fusion reactions, so both reactions were easy to monitor and optimize...
- Human induced pluripotent stem cells free of vector and transgene sequencesJunying Yu
Morgridge Institute for Research, Madison, WI 53707 7365, USA
Science 324:797-801. 2009..These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells...
- Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammalsDorothee Grimm
Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840, USA
Proc Natl Acad Sci U S A 101:3142-7. 2004..The induction of a spirochetal virulence factor preceding the time and host in which it is required demonstrates a developmental sequence for transmission of this arthropod-borne pathogen...
- Improved gfp and inaZ broad-host-range promoter-probe vectorsW G Miller
University of California, Department of Plant and Microbial Biology, Berkeley 94720, USA
Mol Plant Microbe Interact 13:1243-50. 2000..The vector suite was augmented with derivatives of the kanamycin-resistant gfp promoter-probe plasmids that encode Gfp variants with different half-life times.
- MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanismsYan Zeng
Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 100:9779-84. 2003..These data suggest that miRNAs and siRNAs can use similar mechanisms to repress mRNA expression and that the choice of mechanism may be largely or entirely determined by the degree of complementary of the RNA target...
- The genome of Rhizobium leguminosarum has recognizable core and accessory componentsJ Peter W Young
Department of Biology, University of York, York, UK
Genome Biol 7:R34. 2006..We have sequenced the genome of R. leguminosarum biovar viciae strain 3841...
- RF cloning: a restriction-free method for inserting target genes into plasmidsFusinita van den Ent
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
J Biochem Biophys Methods 67:67-74. 2006..The absence of any alterations to the protein as well as the simplicity of both the primer design and the procedure itself makes it suitable for high-throughput expression and ideal for structural genomics...
- Analysis of mutation in human cells by using an Epstein-Barr virus shuttle systemR B DuBridge
Mol Cell Biol 7:379-87. 1987..A total of 33 of 34 lacI nonsense mutations and 26 of 27 missense mutations involve G X C to A X T transitions. These data provide support for the mutational theory of cancer...
- A highly efficient recombineering-based method for generating conditional knockout mutationsPentao Liu
Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA
Genome Res 13:476-84. 2003..systems have recently been developed that now make it possible to subclone or modify DNA cloned into plasmids, BACs, or PACs without the need for restriction enzymes or DNA ligases...
- Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCRF Javier Pérez-Pérez
Center for Research in Anti Infectives and Biotechnology, Department of Medical Microbiology and Immunology, School of Medicine, Creighton University, Omaha, Nebraska 68178, USA
J Clin Microbiol 40:2153-62. 2002..In conclusion, a multiplex PCR technique was developed for identifying family-specific ampC genes responsible for AmpC beta-lactamase expression in organisms with or without a chromosomal AmpC beta-lactamase gene...
- Fitness of Escherichia coli strains carrying expressed and partially silent IncN and IncP1 plasmidsBruce Humphrey
Bristol Centre for Antimicrobial Research, Department of Cellular and Molecular Medicine, University of Bristol, Medical Sciences Building, University Walk, Bristol, BS8 1TD, UK
BMC Microbiol 12:53. 2012Understanding the survival of resistance plasmids in the absence of selective pressure for the antibiotic resistance genes they carry is important for assessing the value of interventions to combat resistant bacteria...
- Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmidsMart Krupovic
Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Departement de Microbiologie, Institut Pasteur, Paris, France
PLoS ONE 8:e49044. 2013..into genetic diversity and dynamics of mobile genetic elements in these environments we have sequenced five new plasmids from different Thermococcus strains that have been isolated from geographically remote hydrothermal vents...
- PSI:Biology-materials repository: a biologist's resource for protein expression plasmidsCatherine Y Cormier
The Virginia G Piper Center for Personalized Diagnostics at the Biodesign Institute at Arizona State University, 1001 S McAllister Ave, Tempe, AZ, 85287 6401, USA
J Struct Funct Genomics 12:55-62. 2011..asu.edu ) sequence-verifies, annotates, stores, and distributes the protein expression plasmids and vectors created by the Protein Structure Initiative (PSI)...
- Histone H3-variant Cse4-induced positive DNA supercoiling in the yeast plasmid has implications for a plasmid origin of a chromosome centromereChu Chun Huang
Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
Proc Natl Acad Sci U S A 108:13671-6. 2011....
- Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutantShital A Tripathi
Mascoma Corporation, Lebanon, NH 03755, USA
Appl Environ Microbiol 76:6591-9. 2010..thermocellum, a microbe that holds an exciting and promising future in the biofuel industry and development of sustainable energy resources...
- A simplified system for generating recombinant adenovirusesT C He
The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA
Proc Natl Acad Sci U S A 95:2509-14. 1998..After transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent ..
- The phage abortive infection system, ToxIN, functions as a protein-RNA toxin-antitoxin pairPeter C Fineran
Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom
Proc Natl Acad Sci U S A 106:894-9. 2009..This is the first demonstration of a novel mechanistic class of TA systems and of an Abi system functioning in different bacterial genera, both with implications for the dynamics of phage-bacterial interactions...
- Pushing and pulling in prokaryotic DNA segregationKenn Gerdes
Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4AX, UK
Cell 141:927-42. 2010..par) locus encodes an actin homolog called ParM, which forms dynamically unstable filaments that push plasmids apart in a process reminiscent of mitosis...
- A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformationKyoung Hee Choi
Department of Microbiology, Immunology and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO 80523, USA
J Microbiol Methods 64:391-7. 2006..Not surprisingly, the method also allows for very efficient transformation with replicative plasmids, with transformation efficiencies ranging from 10(7) to >10(11) transformants per microgram of DNA...
- Nasty viruses, costly plasmids, population dynamics, and the conditions for establishing and maintaining CRISPR-mediated adaptive immunity in bacteriaBruce R Levin
Department of Biology, Emory University, Atlanta, Georgia, United States of America
PLoS Genet 6:e1001171. 2010..mechanism, bacteria with CRISPR regions carrying copies of the DNA of previously encountered phage and plasmids abort the replication of phage and plasmids with these sequences...
- Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombeJ Bahler
Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
Yeast 14:943-51. 1998..We also constructed a series of plasmids containing the kanMX6 module, which allows selection of G418-resistant cells and thus provides a new heterologous ..
- What traits are carried on mobile genetic elements, and why?D J Rankin
Department of Biochemistry, University of Zurich, Zurich, Switzerland
Heredity (Edinb) 106:1-10. 2011....
- Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 cloneNeil Woodford
ARMRL, Centre for Infections, Health Protection Agency, London, United Kingdom
Antimicrob Agents Chemother 53:4472-82. 2009We determined the complete nucleotide sequences of three plasmids that encode CTX-M extended-spectrum beta-lactamases (ESBLs) in pulsed-field gel electrophoresis-defined United Kingdom variants (strains A, C, and D) of the internationally ..
- Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vectorM S Donnenberg
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201
Infect Immun 59:4310-7. 1991..These results confirm the role of the eae gene in the attaching and effacing activity of EPEC and establish the utility of a new system for the construction of deletion mutations...
- Mobility of plasmidsChris Smillie
Institut Pasteur, Microbial Evolutionary Genomics, CNRS, URA2171, F 75015 Paris, France
Microbiol Mol Biol Rev 74:434-52. 2010b>Plasmids are key vectors of horizontal gene transfer and essential genetic engineering tools...
- Creation of a bacterial cell controlled by a chemically synthesized genomeDaniel G Gibson
The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
Science 329:52-6. 2010..The new cells have expected phenotypic properties and are capable of continuous self-replication...
- Environmental genome shotgun sequencing of the Sargasso SeaJ Craig Venter
Institute for Biological Energy Alternatives, 1901 Research Boulevard, Rockville, MD 20850, USA
Science 304:66-74. 2004..2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity...
- Complete genome sequence of Salmonella enterica serovar Typhimurium LT2M McClelland
Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, California 92121, USA
Nature 413:852-6. 2001..Most of these homologues were previously unknown, and 50 may be exported to the periplasm or outer membrane, rendering them accessible as therapeutic or vaccine targets...
- The repertoire of ICE in prokaryotes underscores the unity, diversity, and ubiquity of conjugationJulien Guglielmini
Institut Pasteur, Microbial Evolutionary Genomics, Département Génomes et Génétique, Paris, France
PLoS Genet 7:e1002222. 2011..Conjugation allows the broadest range and the highest gene transfer input per transfer event. While conjugative plasmids have been studied for decades, the number and diversity of integrative conjugative elements (ICE) in prokaryotes ..
- The genome of the natural genetic engineer Agrobacterium tumefaciens C58D W Wood
Department of Microbiology, University of Washington, 1959 NE Pacific Street, Box 357242, Seattle, WA 98195, USA
Science 294:2317-23. 2001..the plant pathogen Agrobacterium tumefaciens C58 consists of a circular chromosome, a linear chromosome, and two plasmids. Extensive orthology and nucleotide colinearity between the genomes of A...
- Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coliB Karsten Tischer
Cornell University, Ithaca, NY, USA
Biotechniques 40:191-7. 2006....
- Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity systemKirill A Datsenko
Waksman Institute, Piscataway, New Jersey 08854, USA
Nat Commun 3:945. 2012..system that functions by acquisition of short fragments of DNA (mainly from foreign invaders such as viruses and plasmids) and subsequent destruction of DNA with sequences matching acquired fragments...
- A dynamic, mitotic-like mechanism for bacterial chromosome segregationMichael A Fogel
Genetics Program, Tufts University School of Medicine, and Howard Hughes Medical Institute, Boston, Massachusetts 02111, USA
Genes Dev 20:3269-82. 2006..These data suggest that ParAI forms a dynamic structure that pulls the ParBI-bound chromosome to the pole in a process analogous to anaphase of eukaryotic mitosis...
- The composite genome of the legume symbiont Sinorhizobium melilotiF Galibert
UMR6061-CNRS, , , , F-35043 Rennes Cedex, France
Science 293:668-72. 2001..The genome sequence will be useful in understanding the dynamics of interkingdom associations and of life in soil environments...
- Epitope tagging of chromosomal genes in SalmonellaS Uzzau
Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France
Proc Natl Acad Sci U S A 98:15264-9. 2001..The procedure described here should be applicable to a wide variety of Gram-negative bacteria and is particularly suited for the study of intracellular pathogens...
- Borrelia burgdorferi OspC protein required exclusively in a crucial early stage of mammalian infectionKit Tilly
Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, MT 59840, USA
Infect Immun 74:3554-64. 2006..We conclude that OspC is indispensable for establishing infection by B. burgdorferi in mammals but is not required at any other point of the mouse-tick infection cycle...
- The ClosTron: Mutagenesis in Clostridium refined and streamlinedJohn T Heap
BBSRC Sustainable BioEnergy Centre, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK
J Microbiol Methods 80:49-55. 2010..com; the out-sourced construction of re-targeted intron plasmids has become cost-effective as well as rapid; and the combination of constitutive intron expression with direct ..
- Dutch patients, retail chicken meat and poultry share the same ESBL genes, plasmids and strainsM A Leverstein-Van Hall
Department of Medical Microbiology, University Medical Centre Utrecht, The Netherlands
Clin Microbiol Infect 17:873-80. 2011..Therefore, distribution of ESBL genes, plasmids and strain genotypes in Escherichia coli obtained from poultry and retail chicken meat in the Netherlands was ..
- The human gut mobile metagenome: a metazoan perspectiveBrian V Jones
Centre for Biomedical and Health Science Research, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK
Gut Microbes 1:415-31. 2010..TRACA system in conjunction with a comparative metagenomic approach, we have recently explored the pool of plasmids associated with the human gut mobile metagenome...
- Complete DNA sequence, comparative genomics, and prevalence of an IncHI2 plasmid occurring among extraintestinal pathogenic Escherichia coli isolatesTimothy J Johnson
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, 1802 Elwood Drive, VMRI 2, Iowa State University, Ames, IA 50011, USA
Antimicrob Agents Chemother 50:3929-33. 2006..coli isolates...
- Novel cassette-based shuttle vector system for gram-positive bacteriaEmmanuelle Charpentier
Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, NYU Medical Center, New York, USA
Appl Environ Microbiol 70:6076-85. 2004..Questions about gene dosage, complementation, and cis-trans effects can now be conveniently addressed, so that this system constitutes an effective tool for studying gene regulation of staphylococci in various ecosystems...
- Site-specific chromosomal integration of large synthetic constructsThomas E Kuhlman
Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544 1014 USA
Nucleic Acids Res 38:e92. 2010....
- Dissemination of blaKPC-2 by the spread of Klebsiella pneumoniae clonal complex 258 clones (ST258, ST11, ST437) and plasmids (IncFII, IncN, IncL/M) among Enterobacteriaceae species in BrazilLeonardo Neves Andrade
Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil
Antimicrob Agents Chemother 55:3579-83. 2011..pneumoniae clonal complex 258 (CC258) clones (ST258, ST11, and ST437) and a diversity of plasmids (IncFII, IncN, and IncL/M, two untypeable plasmids carrying Tn4401a or Tn4401b) successfully disseminated among ..
- IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vectorH Mizuguchi
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan
Mol Ther 1:376-82. 2000..e., care should be taken regarding the decreased capacity of IRES-dependent downstream gene expression as well as in determining which gene should be positioned as the first or second gene in a bicistronic vector...
- De novo assembly using low-coverage short read sequence data from the rice pathogen Pseudomonas syringae pv. oryzaeJosephine A Reinhardt
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Genome Res 19:294-305. 2009..syringae pan-genome, a corresponding contraction of the core genome, and a further diversification of the type III effector complement for this important plant pathogen species...
- Insertion sequences in prokaryotic genomesPatricia Siguier
Laboratoire de Microbiologie et Génétique Moléculaires UMR5100 CNRS Campus Université Paul Sabatier 118, route de Narbonne, F 31062 Toulouse Cedex, France
Curr Opin Microbiol 9:526-31. 2006..g. plasmids and viruses) and the factors which influence this, and appreciation of the different transposition mechanisms in ..
- Generation of mouse induced pluripotent stem cells without viral vectorsKeisuke Okita
Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
Science 322:949-53. 2008..Here, we report the generation of mouse iPS cells without viral vectors. Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3/4, Sox2, and Klf4 and the other containing the c-Myc cDNA, ..
- Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequencesC C Mello
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138
EMBO J 10:3959-70. 1991..Integration of the injected DNA, though relatively rare, was reproducibly achieved when single-stranded oligonucleotide was co-injected with the double-stranded DNA...
- AmpC beta-lactamasesGeorge A Jacoby
Lahey Clinic, Burlington, Massachusetts 01805, USA
Clin Microbiol Rev 22:161-82, Table of Contents. 2009..Transmissible plasmids have acquired genes for AmpC enzymes, which consequently can now appear in bacteria lacking or poorly expressing ..
- Conjugative transfer by the virulence system of Legionella pneumophilaJ P Vogel
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA
Science 279:873-6. 1998..The dot virulence genes encoded a large putative membrane complex that functioned as a secretion system that was able to transfer plasmid DNA from one cell to another...
- The CGView Server: a comparative genomics tool for circular genomesJason R Grant
Department of Agricultural, Food and Nutritional Science, University of Alberta, Canada
Nucleic Acids Res 36:W181-4. 2008..The CGView Server can be accessed at http://stothard.afns.ualberta.ca/cgview_server/..
- Variation in M. tuberculosis in response to host selectionSarah Fortune; Fiscal Year: 2009..In these studies, we expect to provide fundamental insights into the mechanisms and targets of diversifying immune selection in M. tuberculosis. ..
- Regulation of HSV-1 Gene Expression and Replication by Nuclear Hormone ReceptorsSHAOCHUNG VICTOR HSIA; Fiscal Year: 2013..They both exhibit repressive effects on HSV-1 [unreadable] genes. In Aim 2, we will generate a series of mutant plasmids with partial deletion of HSV-1 Repeat Element-1 (RE-1) to study the boundary effect of CTCF on LAT and [..
- Non-viral Genetic Modification of Antigen-presenting Cells in AllograftsWilson S Meng; Fiscal Year: 2011..Successful completion of the study will lead to improved health outcome of a significant number of patients by decreasing the need for indefinite use of non-specific immunosuppressants in transplant recipients. ..
- MOLECULAR BASIS OF REOVIRUS PATHOGENESISTerence S Dermody; Fiscal Year: 2013..abstract_text> ..
- Novel DNA-Launched Attenuated Vaccine for VEE VirusPETER M PUSHKO; Fiscal Year: 2012..If successful, this technology can potentially transform the field of live attenuated vaccines for many viral diseases. ..
- Present Homologous and Heterologous Antigen with Hepatitis E VirusR Holland Cheng; Fiscal Year: 2012....
- Characterization of new toxins (YmgD and YdfD) from E.coli, targeting cell wallHisako Masuda; Fiscal Year: 2012..The first such example was found in plasmids and bacteriophage, and required for "post-segregational killing'(15, 17)...
- CHEMISTRY AND BIOLOGY OF PLATINUM ANTICANCER DRUGSStephen J Lippard; Fiscal Year: 2013..Pt-DNA adducts will be studied with site-specifically modified nucleosomes in vitro and with platinated reporter plasmids in live cells following transfection...
- Regulation of Astrocyte TIMP-1 in HIV-Associated DementiaJEREL ADAM FIELDS; Fiscal Year: 2011..Aim 2 will be completed by combining pathway-specific inhibitors with a panel of luciferase expression plasmids, CAAT-binding factor overexpression plasmids and pathway-indicating plasmids with and without HAD-relevant ..
- Regulation of insulins by the Rhox5 homeobox gene supports spermatogenesisJames Maclean; Fiscal Year: 2009..Transient transfection of Rhox5 expression plasmids increased the activity of Ins2 promoter-driven luciferase reporter constructs...
- Mechanism of Irradiation Pulmonary FibrosisJoel S Greenberger; Fiscal Year: 2012..Project Narrative This irradiation application addresses a critical problem in managing the side effects of thoracic radiotherapy for lung and esophagus cancer. ..
- Regulation of EBV Latency by EBNA1Paul M Lieberman; Fiscal Year: 2013..More detailed understanding of EBNA1 is further justified by its potential value as a target for therapeutic intervention in diseases and cancers associated with EBV latent infection. ..
- DRUG RESISTANCE AND PTERIDINE METABOLISM IN LEISHMANIAStephen M Beverley; Fiscal Year: 2010..These studies have yielded new opportunities for chemotherapeutic attack and new directions for future study of pteridine metabolism and biology. [unreadable] ..
- ENZYMOLOGY OF ANTIBIOTIC RESISTANCERichard Armstrong; Fiscal Year: 2009..Plasmid encoded versions of FosA and FosX have been found in multi-drug resistant plasmids Serratia marcescens and P. putida...
- Chronic Granulomatous Lung Inflammation Elicited by Carbon NanotubesMary Jane Thomassen; Fiscal Year: 2013..numbers in: (a) macrophage-specific PPAR?-null versus wild-type mice;and (b) mice treated with lentivirus-PPAR? plasmids, PPAR? agonist rosiglitazone;or PPAR? antagonist BADGE versus untreated mice...
- The Role of the Nuclear Lamina in Chromatin Organization and Gene Expression in NMarcos Estecio; Fiscal Year: 2012..a method to study the chromatin binding to the nuclear lamina (LAD-Seq) that is independent from engineered plasmids transfection and applicable to primary, uncultured cells...
- Cellular functions of the essential PcrA helicase in Staphylococcus aureusSaleem A Khan; Fiscal Year: 2013..aureus. Since PcrA is a conserved protein, the knowledge gained from these studies could be used in the future for the development of drugs against S. aureus and other Gram-positive human pathogens. ..
- Growth Factor and Antiapoptotic Gene Delivery to Human IsletsRam I Mahato; Fiscal Year: 2012..This will decrease the number of islets required to achieve normoglycemia in type I diabetic patients. ..
- Endemic maintenance of relapsing fever genotypes.Michael Bela Teglas; Fiscal Year: 2013..The bacterium utilizes a reserve of pseudogenes located on multiple plasmids that allow recombinant variants to be expressed during successive waves of spirochetemia...
- Centromere Structure and Function in Candida albicansJudith G Berman; Fiscal Year: 2012..Furthermore, we have constructed CEN-plasmids that will be useful for many applications...
- Disruption of Autonomic Pathways in Left Atrium by Inhibition of G ProteinsAlan H Kadish; Fiscal Year: 2010..We propose to use a new method to treat AF, by inhibiting the function of the nerves that are critical to the genesis of this arrhythmia. By using a new inhibitory protein, we will selectively block these nerves in the left atrium. ..
- Novel Bacillus subtilis based spore vaccine to tuberculosisSubramanian Dhandayuthapani; Fiscal Year: 2013..Finally, we will test whether mice with these immunization groups are protected against challenge doses of live M. tuberculosis H37Rv. ..
- National surveillance of emerging MDR in pediatric Enterobacteriaceae infectionsDanielle M Zerr; Fiscal Year: 2013..and is concerning both because it limits the antimicrobials available to treat infections and because plasmids are readily transferable between organisms, creating a threat for rapid spread of resistance...
- Multiplex Serodiagnostic Protein MicroarrayPHILIP LOUIS FELGNER; Fiscal Year: 2012..Deliverables generated from this project include plasmids, customized protein microarrays in a modular multiplex format, purified recombinant proteins and lateral flow (..
- Defining evolutionary trajectories: Molecular adaptation to antibiotic resistanceADIL YOUSIF SHAMOO; Fiscal Year: 2012..Mobile genetic elements such as transposons, conjugative transposons and plasmids act as vectors between microbial populations and can spread resistance beyond the agricultural or clinical ..
- Structures of RNA processing and Silencing Enzymes in ProkaryotesHong Li; Fiscal Year: 2013..a variety of defense systems to protect themselves from selfish genetic elements (phages, transposons, and plasmids)...
- HTLV-1 &Cellular Factors in Neuroinflammatory DiseaseBrian Wigdahl; Fiscal Year: 2013..to interact with these factors independently have been performed using transiently transfected viral reporter plasmids or in cell lines that do not represent the primary target for HTLV-1 in vivo...
- Regulations of DNA Alkylation/Deamination Damage RepairRabindra Roy; Fiscal Year: 2013..Furthermore, this knowledge will allow us eventually to devise strategies for modulating MPG expression for chemopreventive and therapeutic purposes. ..
- Genomic profiling of yeast resistance to AFB1, a P450-activated carcinogenMICHAEL THOMAS FASULLO; Fiscal Year: 2013..We will introduce plasmids that express human CYP1A2 into the ~5,000 diploid homozygous single-deletion and heterozygous single-deletion ..
- Role of H-Ras in Retinal Cell Death in DiabetesRenu A Kowluru; Fiscal Year: 2013..The central hypothesis will be tested using active plasmids, siRNAs and pharmacological inhibitors in isolated cells, and in vitro findings will be validated in in vivo ..
- Development of transformed lactobacilli as a microbicideRichard B Markham; Fiscal Year: 2010..Those VHH will then be expressed from plasmids and/or the bacterial chromosome of Lactobacillus rhamnosus GR-1 and Streptococcus gordonii...
- C/EBP, atrogin-1, and muscle wastingPer Olof Hasselgren; Fiscal Year: 2009..protein degradation and expression of atrogin-1 and MuRF1 is tested by transfecting cultured muscle cells with plasmids expressing wild-type C/EBP[unreadable] or C/EBP[unreadable] with mutations of specific acetylation or ..
- Biophysics of Drug Interactions with Viral EpisomesJames K Bashkin; Fiscal Year: 2013....
- Development of CNS-targeted AAV vectorsMIGUEL S ESTEVES; Fiscal Year: 2012..of CNS-targeted AAV vectors to foster the development of highly effective gene therapy approaches to treat many childhood, adult, and geriatric neurological diseases currently beyond the reach of modern medicine ..
- Role of TRPM1 (Melastatin1) in the Biology of Human MelanocytesVijayasaradhi Setaluri; Fiscal Year: 2012..To address these issues, we generated TRPM1 expression (constitutive and tet-inducible) shRNA plasmids and antibody reagents...
- Advancing a Lead Candidate Hantavirus Pulmonary Syndrome DNA VaccineJAY WILLIAMS HOOPER; Fiscal Year: 2013..We have developed a candidate HPS vaccine. The vaccine is a DNA vaccine consisting of two plasmids that encode the surface glycoproteins of ANDV and SNV...
- Disruption of Autonomic Pathways in the Left Atrium by Inhibition of G-proteinsRishi Arora; Fiscal Year: 2013..Using minigenes (plasmids) that can express these G-protein inhibitory peptides on both a short and long term basis, the proposed studies ..
- Delivery of biologically active nucleic acids to epidermal cellsROGER L contact KASPAR; Fiscal Year: 2010..If the goal of this work succeeds, it is expected that the pace of new research and development of nucleic treatments for skin disease will accelerate greatly, spawning many new jobs and small companies. ..
- Hepatic Non-viral Gene TherapyLeaf Huang; Fiscal Year: 2013..S/MAR sequence will be incorporated into the IFN-[unreadable]2b plasmid to prolong the gene expression. The goal is to develop a once-a-month IFN gene therapy for the HBV hepatitis patients. ..
- Feedback and Hormonal Regulation of Hepatic HMG-CoA ReductaseGENE CHARLES NESS; Fiscal Year: 2010..examined by either over expressing hepatic HMGR or by knocking it down with hydrodynamic tail vein injections of plasmids containing HMGR cDNA or siRNA...
- Development of Site-Specific Mutagenesis in Coxiella burnettiJAMES EVANS SAMUEL; Fiscal Year: 2012..The proposed project outlined several strategies to develop genetic tools for characterization of gene functions and will aid the development of a vaccine as national security goal. ..
- Polymorphisms of estrogen-metabolizing genes in kidney cancerYuichiro Tanaka; Fiscal Year: 2013..The following experiments will be performed: (a) Development of EMG wild-type plasmids by cloning and generation of polymorphic variants using site-directed mutagenesis, (b) Transfection of promoter ..
- Development of Small Heat Shock Proteins as Therapeutic Agents in the EyeJONATHAN MARK PETRASH; Fiscal Year: 2010..Our proposed studies could substantially improve the quality of life of affected patients by preventing vision loss and associated financial burdens due to lost productivity and increased medical expenses. ..
- Interactions between P. aeruginosa and autophagy in Airway epithelial cellsINDHIRA H DE LA ROSA; Fiscal Year: 2013..In addition, we have received plasmids encoding some of the P. aeruginosa T3SS effector proteins (5, 6), which are responsible for the T3SS toxicity (5)...
- Role of Polyamine Oxidase in Apoptosis Studies with RNA InterferenceGLENN KUEHN; Fiscal Year: 2009....
- CONTROL OF BACTERIAL TOXINS BY VIRUSES AND PLASMIDSRandall K Holmes; Fiscal Year: 2013..tuberculosis and potential value as chemotherapeutic agents for treatment of tuberculosis. ..
- Nontoxigenic Clostridium difficile as an Oral Vaccine for C. difficile InfectionDALE NICHOLAS GERDING; Fiscal Year: 2013..abstract_text> ..
- Functional role of RAG2 H3K4me3 interaction in V(D)J recombinationMarjorie A Oettinger; Fiscal Year: 2013..Our goal is to understand the normal steps in development and how errors in the process lead to immune deficiency syndromes and lymphoid tumors, with the hope of preventing or treating such diseases. ..
- Mechanisms and regulation of serine resolvasesPhoebe A Rice; Fiscal Year: 2012..Sin is encoded by many large multi-resistance plasmids of Staph. aureus, and is thought to promote their stable maintenance by resolving replicon dimers into monomers...
- Intracellular immunization strategy in inhibit HCV related liver cancerSrikanta Dash; Fiscal Year: 2010..If this project becomes successful then it can lead to a potential therapy to treat chronic hepatitis C infection and prevent liver cancer. ..
- HCV in AlcoholicsJack R Wands; Fiscal Year: 2011..this inhibitory effect of ethanol was completely reversed by co-immunization with an IL2 or GM- CSF expression plasmids following genetic immunization suggesting that dendritic cells (DCs) could be involved in these abnormal immune ..
- Targeting stress-mediated pathways in the treatment of muscle insulin resistancePaige C Geiger; Fiscal Year: 2013....
- Structure and mechanism of CRISPR interference.Ailong Ke; Fiscal Year: 2013..CRISPR drives adaptation to harmful invading nucleic acids - such as conjugative plasmids, transposable elements and phages - using an RNA-mediated interference (CRISPR interference) mechanism with ..
- NEUROBIOLOGY OF VARICELLA ZOSTER VIRUSDonald Gilden; Fiscal Year: 2013....
- LANA and Cellular Gene ExpressionROLF F RENNE; Fiscal Year: 2013..More specifically, we study how LANA supports the replication of the viral genome and how it helps to recruit the above mentioned factors. ..
- Plasmid-Mediated Quinolone ResistanceGeorge A Jacoby; Fiscal Year: 2013..gram-negative bacteria from around the world and to be typically incorporated into integrons on multiresistance plasmids. This resubmission application builds on our prior studies to obtain a deeper and more detailed understanding of ..
- CRISPR Cas genes and Legionella pneumophila infectionNicholas P Cianciotto; Fiscal Year: 2013..Thus, by using Lpn a a new model organism, we will probe for novel functions of the CRISPR/Cas locus that seek to shift current research paradigms. ..
- Toxoid adjuvant CRM197 production in a stable reduced genome E. coli strainFrederick R Blattner; Fiscal Year: 2012..These improvements could have a wide impact on production of pharmaceutical protein products and ultimately broaden access to vaccines for at-risk populations who need them. ..
- Targeted DNA Nanoparticles in BrainDavid M Yurek; Fiscal Year: 2010..DNA compacting techniques will be used to form nanoparticles containing condensed DNA plasmids with diameters in the range of 8-12 nanometers...
- Development of Breast Cancer VaccinesWEI ZEN NONE WEI; Fiscal Year: 2013..to Her-2, we formulated DNA vaccines that express both human Her-2 and heterologous rat Neu sequences in separate plasmids or as single hybrid constructs that encode Her-2/neu fusion proteins...
- Improvement of Organ Function after Severe HypovolemiaPing Wang; Fiscal Year: 2013..These proposed studies should open up a new research field for the development of innovative therapeutic agents as resuscitation adjuncts for victims with traumatic hemorrhage. ..
- DNA Pro-Drug Technology in Localized Wound Healing and Tissue RegenerationArthur A Decarlo; Fiscal Year: 2011....
- PAIRING OF HOMOLOGOUS SEQUENCES IN DROSOPHILAKENT GOLIC; Fiscal Year: 2002..The FLP recombinase will be supplied in the form of a synthetic mRNA coinjected with the plasmid library. Plasmids containing sequences promoting plasmid/chromosome pairing should be favored for integration; these sequences ..
- MODELS OF IN VIVO MYOMETRIAL GENE TRANSFERGregory Christman; Fiscal Year: 2001..Together, these studies will provide in vivo efficacy and safety data for the future clinical application of gene therapy in uterine myometrium. ..
- Bacterial heme transport and hemoglobin expressionDouglas Henderson; Fiscal Year: 2009..Our hypothesis is that hemoglobin production can be increased by transforming E. coli with plasmids containing human hemoglobin genes and heme transport genes from a Gram-negative bacterium, and supplying heme in ..
- ANTIBIOTIC SUSCEPTIBILITIES OF PERIODONTAL BACTERIAClay Walker; Fiscal Year: 1993..intermedius and E. corrodens to determine if plasmids encode for resistance in these organisms...
- Mechanisms of Sequence-Based Resistance to Viruses and Plasmids in EubacteriaErik J Sontheimer; Fiscal Year: 2010..the locus suggests that it specifies resistance not only to bacteriophages but also to staphylococcal conjugative plasmids. Given the clinical importance of staphylococci and the experimental tractability of S...
- Pilot trial of a DNA vaccine encoding PAP in patients with prostate cancerDouglas McNeel; Fiscal Year: 2009..This is consequently relevant to the development of other anti-tumor vaccines. ..
- Prevention of neointimal thickening by Mst1Junichi Sadoshima; Fiscal Year: 2006..stents made of noncombustible magnesium and a proprietary technique to coat the stents with mammalian expression plasmids, which can be efficiently delivered to vascular smooth muscle cells (VSMCs), the technique successfully utilized ..
- Regulated Osteochondrogenesis of Human Mesenchymal Stem Cells Using Gene DeliveryAntonios Mikos; Fiscal Year: 2009..Each of these plasmids will be complexed with a novel polymeric gene delivery vector developed in our laboratory (a conjugate of ..
- VIRULENCE IN COXIELLA BURNETTIIHERBERT THOMPSON; Fiscal Year: 1999..In aim 1, organisms will be transformed to ampicillin resistance using a mixture of plasmids en masse via electroporation. These plasmids carry the C...
- IMMUNE CONTROL OF CAE LENTIVIRUSDonald Knowles; Fiscal Year: 2005..Therefore, the second objective of this proposal is to evaluate virus replication following CAEV challenge of goats co-immunized with plasmid DNA expressing CAEV env and tat genes and caprine IFN. ..
- Prevention of diabetes by pDNA encoded with IL-10Jagdish Singh; Fiscal Year: 2010..The proposed study will contribute towards the development of a high efficiency and low toxicity non-viral gene delivery vehicle in order to deliver plasmid encoding IL-10 gene for prevention of type 1 diabetes. ..
- STRUCTURE AND GENETIC CONTROL OF COLICINESDonald Helinski; Fiscal Year: 2005..along with the plasmid specific initiation protein in the initiation of replication of RK2 and narrow-host-range plasmids P1 and F will be determined...
- Structural mechanism of DNA segregation by the pSK41 par systemMaria Schumacher; Fiscal Year: 2010..Essential to this process are the accurate movement and positioning of chromosomes and plasmids to daughter cells at cell division...
- Y ENTEROCOLITICA VIRULENCE PLASMIDJohn Morris; Fiscal Year: 1991....
- Plasmid pXO2 Replication in Bacillus anthracisSaleem Khan; Fiscal Year: 2005Bacillus anthracis is an important human pathogen and a potential biological weapon. Two large plasmids, pXO1 and pXO2 play a major role in the virulence of this organism...