post transcriptional rna processing
Summary: Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein.
Publications295 found, 100 shown here
- The Microprocessor complex mediates the genesis of microRNAsRichard I Gregory
The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
Nature 432:235-40. 2004..In vivo knock-down and in vitro reconstitution studies revealed that both components of this smaller complex, termed Microprocessor, are necessary and sufficient in mediating the genesis of miRNAs from the primary miRNA transcript...
- The Drosha-DGCR8 complex in primary microRNA processingJinju Han
School of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151 742, Korea
Genes Dev 18:3016-27. 2004..Based on these results, we propose a model for the action mechanism of class II RNase III proteins...
- A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNAG Hutvagner
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Science 293:834-8. 2001..Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression...
- A feedback loop comprising lin-28 and let-7 controls pre-let-7 maturation during neural stem-cell commitmentAgnieszka Rybak
Institute of Cell and Neurobiology, Center for Anatomy, Charite Universitatsmedizin Berlin, Berlin, Germany
Nat Cell Biol 10:987-93. 2008....
- The nuclear RNase III Drosha initiates microRNA processingYoontae Lee
Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
Nature 425:415-9. 2003..Thus, the two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation...
- Selective blockade of microRNA processing by Lin28Srinivas R Viswanathan
Stem Cell Program, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
Science 320:97-100. 2008..Our results identify Lin28 as a negative regulator of miRNA biogenesis and suggest that Lin28 may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers...
- MicroRNAs: small RNAs with a big role in gene regulationLin He
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nat Rev Genet 5:522-31. 2004
- RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervalsP D Zamore
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655, USA
Cell 101:25-33. 2000..Cleavage occurs at sites 21-23 nucleotides apart, the same interval observed for the dsRNA itself, suggesting that the 21-23 nucleotide fragments from the dsRNA are guiding mRNA cleavage...
- Processing of primary microRNAs by the Microprocessor complexAhmet M Denli
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nature 432:231-5. 2004..Considered together, these results indicate a role for Pasha in miRNA maturation and miRNA-mediated gene regulation...
- Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAsXuezhong Cai
Box 3025, Duke University Medical Center, Durham, NC 27710, USA
RNA 10:1957-66. 2004..Together, these data show that human pri-miRNAs are not only structurally similar to mRNAs but can, in fact, function both as pri-miRNAs and mRNAs...
- A PUF family portrait: 3'UTR regulation as a way of lifeMarvin Wickens
Dept of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
Trends Genet 18:150-7. 2002..Here, we discuss the evolution, biological function and mechanisms of action of the PUF protein family, and suggest that a primordial function of PUF proteins is to sustain mitotic proliferation of stem cells...
- MicroRNA maturation: stepwise processing and subcellular localizationYoontae Lee
Institute of Molecular Biology and Genetics and School of Biological Science, Seoul National University, Seoul 151 742, Korea
EMBO J 21:4663-70. 2002..Our study suggests that the regulation of miRNA expression may occur at multiple levels, including the two processing steps and the nuclear export step. These data will provide a framework for further studies on miRNA biogenesis...
- SMAD proteins control DROSHA-mediated microRNA maturationBrandi N Davis
Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
Nature 454:56-61. 2008....
- Lin28 mediates the terminal uridylation of let-7 precursor MicroRNAInha Heo
National Creative Research Center and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
Mol Cell 32:276-84. 2008..The Lin28-mediated downregulation of let-7 may play a key role in development, stem cell programming, and tumorigenesis...
- Pre-18S ribosomal RNA is structurally compacted into the SSU processome prior to being cleaved from nascent transcripts in Saccharomyces cerevisiaeYvonne N Osheim
Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908, USA
Mol Cell 16:943-54. 2004..The results show that the approximately 40 components of the SSU processome/90S preribosome can complete their tasks within approximately 85 s in optimal conditions...
- From birth to death: the complex lives of eukaryotic mRNAsMelissa J Moore
Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, 415 South Street, Waltham, MA 02454
Science 309:1514-8. 2005..Thus, a comprehensive understanding of eukaryotic gene expression requires an appreciation for how the lives of mRNAs are influenced by a wide array of diverse regulatory mechanisms...
- Human 5' --> 3' exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sitesSteven West
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Nature 432:522-5. 2004..Degradation of the downstream cleavage product by Xrn2 results in transcriptional termination, as envisaged in the torpedo model...
- The C-terminal domain of RNA polymerase II couples mRNA processing to transcriptionS McCracken
Amgen Institute, Toronto, Ontario, Canada
Nature 385:357-61. 1997....
- Processing of intronic microRNAsYoung Kook Kim
Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea
EMBO J 26:775-83. 2007..Our study provides a novel example of eukaryotic gene organization and RNA-processing control...
- Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positionsYoshiho Ikeuchi
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
Mol Cell 21:97-108. 2006....
- Modulation of microRNA processing and expression through RNA editing by ADAR deaminasesWeidong Yang
The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
Nat Struct Mol Biol 13:13-21. 2006..Consequently, mature miRNA-142 expression levels increased substantially in ADAR1 null or ADAR2 null mice. Our results demonstrate a new function of RNA editing in the control of miRNA biogenesis...
- CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thalianaWonkeun Park
Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
Curr Biol 12:1484-95. 2002....
- Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complexJinju Han
School of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University, Seoul
Cell 125:887-901. 2006..Our current study thus facilitates the prediction of novel microRNAs and will assist in the rational design of small hairpin RNAs for RNA interference...
- The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limbBrian D Harfe
Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
Proc Natl Acad Sci U S A 102:10898-903. 2005..Thus, Dicer is required for the formation of normal mouse limbs. Strikingly, however, we did not detect defects in basic patterning or in tissue-specific differentiation of Dicer-deficient limb buds...
- RNA editing of AMPA receptor subunit GluR-B: a base-paired intron-exon structure determines position and efficiencyM Higuchi
Center for Molecular Biology, University of Heidelberg, Federal Republic of Germany
Cell 75:1361-70. 1993..Base conversion in the channel-coding region of GluR-B directed by base paired sequences may be executed by a ubiquitous nuclear adenosine deaminase specific for double-stranded RNA...
- Mechanisms of gene silencing by double-stranded RNAGunter Meister
Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
Nature 431:343-9. 2004..These short dsRNAs guide RNA silencing by specific and distinct mechanisms. Many components of the RNA silencing machinery still need to be identified and characterized, but a more complete understanding of the process is imminent...
- Single processing center models for human Dicer and bacterial RNase IIIHaidi Zhang
Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
Cell 118:57-68. 2004..Based on these and other data, we propose that Dicer functions through intramolecular dimerization of its two RNase III domains, assisted by the flanking RNA binding domains, PAZ and dsRBD...
- Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhBTaras Afonyushkin
Max F Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter Dr Bohrgasse 9 4, A 1030 Vienna, Austria
Nucleic Acids Res 33:1678-89. 2005..These data are discussed in terms of a model, which accounts for the observed roles of RNase E and RNase III in sodB mRNA turnover...
- Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeastAndre P Gerber
Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
PLoS Biol 2:E79. 2004....
- RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome componentsJennifer E G Gallagher
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520 8024, USA
Genes Dev 18:2506-17. 2004..This association may thus represent an early step in the formation of the SSU processome. Our results indicate that rRNA transcription and pre-rRNA processing are coordinated via specific components of the SSU processome...
- MicroRNAS and their regulatory roles in plantsMatthew W Jones-Rhoades
Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, and Howard Hughes Medical Institute, Cambridge, Massachusetts 02142, USA
Annu Rev Plant Biol 57:19-53. 2006....
- An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNAAkiko Soma
Department of Life Science, College of Science, Rikkyo St Paul s University, 3 34 1 Nishi Ikebukuro, Toshima ku, Tokyo 171 8501, Japan
Mol Cell 12:689-98. 2003..Partial inactivation of tilS in vivo resulted in an AUA codon-dependent translational defect, which supports the notion that TilS is an RNA-modifying enzyme that plays a critical role in the accurate decoding of genetic information...
- Precision and functional specificity in mRNA decayYulei Wang
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
Proc Natl Acad Sci U S A 99:5860-5. 2002..The results provide strong evidence that precise control of the decay of each mRNA is a fundamental feature of the gene expression program in yeast...
- RNA regulons: coordination of post-transcriptional eventsJack D Keene
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3020, Durham, North Carolina 27710, USA
Nat Rev Genet 8:533-43. 2007..I close by considering the evolutionary wiring and rewiring of these combinatorial post-transcriptional gene-expression networks...
- RNase G (CafA protein) and RNase E are both required for the 5' maturation of 16S ribosomal RNAZ Li
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33101, USA
EMBO J 18:2878-85. 1999..These data indicate that both RNase E and CafA protein are required for a two step, sequential maturation of the 5' end of 16S rRNA, and that CafA protein is a new ribonuclease. We propose that it be renamed RNase G...
- Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistanceP Mourrain
Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Versailles, France
Cell 101:533-42. 2000..Both sgs2 and sgs3 mutants show enhanced susceptibility to virus, definitively proving that PTGS is an antiviral defense mechanism that can also target transgene RNA for degradation...
- RNA quality control: degradation of defective transfer RNAZhongwei Li
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33101 6129, USA
EMBO J 21:1132-8. 2002..These data indicate that quality control of stable RNA metabolism in many ways resembles normal turnover of unstable RNA...
- Widespread A-to-I RNA editing of Alu-containing mRNAs in the human transcriptomeAlekos Athanasiadis
Department of Biological Sciences, Lehigh University Bethlehem, Pennsylvania, USA
PLoS Biol 2:e391. 2004..The analysis suggests that modification of repetitive elements is a predominant activity for RNA editing with significant implications for cellular gene expression...
- Increased efficiency of mRNA 3' end formation: a new genetic mechanism contributing to hereditary thrombophiliaN H Gehring
Department of Pediatrics, , Humboldt University, Berlin, Germany
Nat Genet 28:389-92. 2001..This work also illustrates the pathophysiologic importance of quantitatively minor aberrations of RNA metabolism...
- Functions of the exosome in rRNA, snoRNA and snRNA synthesisC Allmang
Institute of Cell and Molecular Biology, University of Edinburgh, Swann Building, King s Buildings, Edinburgh EH9 3JR, UK
EMBO J 18:5399-410. 1999..We conclude that the exosome is involved in the processing of many RNA substrates and that different components can have distinct functions...
- The 7472insC mitochondrial DNA mutation impairs the synthesis and extent of aminoacylation of tRNASer(UCN) but not its structure or rate of turnoverMarina Toompuu
Institute of Medical Technology and Tampere University Hospital, FIN 33014 University of Tampere, Finland
J Biol Chem 277:22240-50. 2002..These findings indicate a common molecular process underlying sensorineural deafness caused by mitochondrial tRNA(Ser(UCN)) mutations...
- Global analysis of Pub1p targets reveals a coordinate control of gene expression through modulation of binding and stabilityRadharani Duttagupta
Department of Molecular Genetics, Microbiology, and Immunology, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Ln, Piscataway, New Jersey 08854 5627, USA
Mol Cell Biol 25:5499-513. 2005..These results suggest that Pub1p binds to discrete subsets of cellular transcripts and posttranscriptionally regulates their expression at multiple levels...
- Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylaseTakashi Nagaike
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba Prefecture, Japan
J Biol Chem 280:19721-7. 2005..These results demonstrate that the poly(A) length of human mt mRNAs is controlled by polyadenylation by hmtPAP and deadenylation by hPNPase, and polyadenylation is required for the stability of mt mRNAs...
- Yeast Nfs1p is involved in thio-modification of both mitochondrial and cytoplasmic tRNAsYumi Nakai
Department of Biochemistry, Osaka Medical College, 2 7 Daigaku machi, Takatsuki, Osaka 569 8686, Japan
J Biol Chem 279:12363-8. 2004..These results suggest that Nfs1p is involved in the 2-thio-modification of both 5-carboxymethylaminomethyl-2-thiouridine in mt-tRNAs and mcm(5)s(2)U in cy-tRNAs...
- The human histone gene expression regulator HBP/SLBP is required for histone and DNA synthesis, cell cycle progression and cell proliferation in mitotic cellsXiujie Zhao
School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK
J Cell Sci 117:6043-51. 2004..These findings indicate that human HBP/SLBP is essential for the coordinate synthesis of DNA and histone proteins and is required for progression through the cell division cycle...
- Oncogenic All1 fusion proteins target Drosha-mediated microRNA processingTatsuya Nakamura
Department of Molecular Virology, Immunology, and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210
Proc Natl Acad Sci U S A 104:10980-5. 2007..Finally, we present evidence from ChIP experiments for All1 fusion protein-mediated recruitment of Drosha to target genes encoding miRNAs. Such recruitment may underlie the enhanced expression of the relevant miRNAs...
- A protein complex mediating mRNA degradation in Escherichia coliB Py
Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, Oxford, UK
Mol Microbiol 14:717-29. 1994..The identification of a complex which includes several activities associated with mRNA degradation has implications for the mechanisms and co-ordinated control of mRNA degradation...
- Ultraconserved elements are associated with homeostatic control of splicing regulators by alternative splicing and nonsense-mediated decayJulie Z Ni
Center for Molecular Biology of RNA and Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz, Santa Cruz, California 95064, USA
Genes Dev 21:708-18. 2007....
- Mechanisms of microRNA-mediated gene regulation in animal cellsTimothy W Nilsen
Center for RNA Molecular Biology, Case Western Reserve University, School of Medicine, W127, 10900 Euclid Avenue Cleveland, OH 44106 4973, USA
Trends Genet 23:243-9. 2007..In this review, I describe the evidence that there are multiple mechanisms of microRNA-mediated repression and discuss the possible connections between these mechanisms...
- Heterogeneity in polyadenylation cleavage sites in mammalian mRNA sequences: implications for SAGE analysisE Pauws
Laboratory of Pediatric Endocrinology and Bioinformatics Laboratory, Academic Medical Center, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands
Nucleic Acids Res 29:1690-4. 2001..Both experimental and in silico data show that the selection of the specific cleavage site for poly(A) addition using a given polyadenylation signal is more variable than was previously thought...
- The nuclear exosome contributes to autogenous control of NAB2 mRNA levelsKelly M Roth
Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Box 672, 601 Elmwood Ave, Rochester, NY 14642, USA
Mol Cell Biol 25:1577-85. 2005..These findings suggest a novel regulatory circuit in which the nuclear exosome controls the level of NAB2 mRNA in response to changes in the activity of Nab2 protein...
- Extensive post-transcriptional regulation of microRNAs and its implications for cancerJ Michael Thomson
Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Genes Dev 20:2202-7. 2006..These data uncover a novel regulatory step in miRNA function and provide a mechanism for miRNA down-regulation in cancer...
- Bop1 is a mouse WD40 repeat nucleolar protein involved in 28S and 5. 8S RRNA processing and 60S ribosome biogenesisZ Strezoska
Department of Molecular Genetics, University of Illinois at Chicago College of Medicine, Chicago, Illinois 60607 7170, USA
Mol Cell Biol 20:5516-28. 2000..These studies thus identify Bop1 as a novel, nonribosomal mammalian protein that plays a key role in the formation of the mature 28S and 5.8S rRNAs and in the biogenesis of the 60S ribosomal subunit...
- LIN-28 and the poly(U) polymerase PUP-2 regulate let-7 microRNA processing in Caenorhabditis elegansNicolas J Lehrbach
Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, The Henry Wellcome Building of Cancer and Developmental Biology, Cambridge, UK
Nat Struct Mol Biol 16:1016-20. 2009..Uridylation by a PUP-2 ortholog might regulate let-7 and additional miRNAs in other species. Given the roles of Lin28 and let-7 in stem cell and cancer biology, we propose that such poly(U) polymerases are potential therapeutic targets...
- Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome functionC Condon
Unité Propre de Recherche 9073, Institut de Biologie Physico Chimique, Paris, France
RNA 7:242-53. 2001..A potential correlation between the presence of RNase E or RNase M5 and the single- or double-stranded nature of the predicted cleavage sites is explored...
- Single nucleotide polymorphism associated with mature miR-125a alters the processing of pri-miRNARanhui Duan
Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA
Hum Mol Genet 16:1124-31. 2007..Thus, our study reveals an additional structural requirement for pri-miRNA processing and emphasizes the importance of identifying new miRNA SNPs and their contributions to miRNA biogenesis and human genetic disease...
- The Saccharomyces cerevisiae RNase mitochondrial RNA processing is critical for cell cycle progression at the end of mitosisTi Cai
Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, 50 E Adams Street, Syracuse, NY 13210, USA
Genetics 161:1029-42. 2002..These results indicate a clear role of RNase MRP in cell cycle progression at the end of mitosis. Conservation of this function in humans may explain many of the pleiotropic phenotypes of cartilage hair hypoplasia...
- Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATPHaidi Zhang
Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH 4002 Basel, Switzerland
EMBO J 21:5875-85. 2002..Additional experiments suggest that if ATP participates in the Dicer reaction in mammalian cells, it might be involved in product release needed for the multiple turnover of the enzyme...
- Pseudouridine synthasesTomoko Hamma
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, Washington 98109, USA
Chem Biol 13:1125-35. 2006....
- A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coliY Komine
Department of Biophysics, Faculty of Science, Kyoto University, Japan
Proc Natl Acad Sci U S A 91:9223-7. 1994..A pair could be overcome by the introduction of a gene encoding alanyl-tRNA synthetase (alaS) on a multicopy plasmid. The evidence suggests that the proposed structural features of 10Sa RNA are indeed manifested in vivo...
- Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translationJohannes H Urban
Max Planck Institute for Infection Biology, RNA Biology Group, Berlin, Germany
PLoS Biol 6:e64. 2008..Our study reveals hierarchical action of two well-conserved sRNAs in a complex regulatory cascade that controls the glmS mRNA. Similar cascades of noncoding RNA regulators may operate in other organisms...
- tRNA transfers to the limelightAnita K Hopper
Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
Genes Dev 17:162-80. 2003
- The N-terminal double-stranded RNA binding domains of Arabidopsis HYPONASTIC LEAVES1 are sufficient for pre-microRNA processingFeijie Wu
National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
Plant Cell 19:914-25. 2007..We suggest that the N-terminal dsRBDs fulfill the function of the whole HYL1 and thus play an essential role in miRNA processing and miRNA-directed silencing of targeted genes...
- Processing of the precursors to small nucleolar RNAs and rRNAs requires common componentsE Petfalski
Institute of Cell and Molecular Biology, University of Edinburgh, United Kingdom
Mol Cell Biol 18:1181-9. 1998..8S rRNA. Processing of the pre-rRNA and both intronic and polycistronic snoRNAs therefore involves common components...
- A conserved small RNA promotes discoordinate expression of the glmUS operon mRNA to activate GlmS synthesisJohannes H Urban
Max Planck Institute for Infection Biology, RNA Biology, Chariteplatz 1, 10117 Berlin, Germany
J Mol Biol 373:521-8. 2007..Our data suggest that Gram-negative bacteria evolved a mechanism of glmS riboregulation that is distinct from the glmS riboswitch mechanism of Gram-positive bacteria...
- Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomesTiffany D Miles
Department of Biological Sciences, Carnegie Mellon University, 616 Mellon Institute, 4400 Fifth Ave, Pittsburgh, PA 15213, USA
Mol Cell Biol 25:10419-32. 2005..Mutations in the WD40 motifs of Ytm1 disrupt binding to Erb1, destabilize the heterotrimer, and delay pre-rRNA processing and nuclear export of preribosomes. Nevertheless, 66S preribosomes lacking Ytm1 remain otherwise intact...
- Cold shock induction of RNase R and its role in the maturation of the quality control mediator SsrA/tmRNAFatima Cairrao
Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apt 127, 2781 901 Oeiras, Portugal
Mol Microbiol 50:1349-60. 2003..The wide biological significance of RNase R regarding adaptation to cold shock and its involvement in RNA surveillance, protein quality control and pathogenesis is discussed...
- Ribonuclease E provides substrates for ribonuclease P-dependent processing of a polycistronic mRNAP Alifano
Dipartimento di Biologia e Patologia Cellulare e Molecolare L Califano, Universita Degli Studi Di Napoli, Italy
Genes Dev 8:3021-31. 1994..This is the first evidence that ribonuclease P, an essential ribozyme required for the biosynthesis of tRNA, may also be involved in the segmental stabilization of a mRNA...
- Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterningIan R Henderson
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California 90095, USA
Nat Genet 38:721-5. 2006..Our studies also suggest that asymmetric DNA methylation can be maintained by pathways that do not require siRNAs...
- Sequence requirements for micro RNA processing and function in human cellsYan Zeng
Howard Hughes Medical Institute, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
RNA 9:112-23. 2003..These results suggest that miRNAs, and the closely similar small interfering RNAs, cannot totally discriminate between RNA targets differing by a single nucleotide...
- Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAsJürgen Soutschek
Alnylam Europe AG, Fritz Hornschuch Str 9, 95326 Kulmbach, Germany
Nature 432:173-8. 2004..These findings demonstrate the therapeutic potential of siRNAs for the treatment of disease...
- Identification of modified residues in RNAs by reverse transcription-based methodsYuri Motorin
Laboratoire de Maturation des ARN et Enzymologie Moleculaire, Faculte des Sciences et Techniques, Nancy Universite, Vandouevre les Nancy, France
Methods Enzymol 425:21-53. 2007..Advice for an optimized use of these methods and for correct interpretation of the data is also given. We also provide some helpful information on the ability of other naturally occurring modified nucleotides to generate RT pauses...
- The putative NTPase Fap7 mediates cytoplasmic 20S pre-rRNA processing through a direct interaction with Rps14Sander Granneman
Yale University School of Medicine, Department of Molecular Biophysics and Biochemistry, 333 Cedar Street, SHM C 114, New Haven, CT 06520 8024, USA
Mol Cell Biol 25:10352-64. 2005..We propose that Fap7 mediates cleavage of the 20S pre-rRNA at site D by directly interacting with Rps14 and speculate that it is an enzyme that functions as an NTP-dependent molecular switch in 18S rRNA maturation...
- RNase MRP cleaves the CLB2 mRNA to promote cell cycle progression: novel method of mRNA degradationTina Gill
Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
Mol Cell Biol 24:945-53. 2004..In addition, these results denote a new mechanism of mRNA degradation not seen before in the yeast Saccharomyces cerevisiae...
- Stress granules and processing bodies are dynamically linked sites of mRNP remodelingNancy Kedersha
Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
J Cell Biol 169:871-84. 2005..We propose that mRNA released from disassembled polysomes is sorted and remodeled at SGs, from which selected transcripts are delivered to PBs for degradation...
- RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1Brigitte Pertschy
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Biol Chem 284:35079-91. 2009..In ltv1/pfa1 mutants the 20 S pre-rRNA was susceptible to 3' to 5' degradation by the cytoplasmic exosome. This degraded into the 3' region of the 18 S rRNA, strongly indicating that the preribosomes are structurally defective...
- Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43pN I Zanchin
Department of Biology, University of Rochester, Rochester, New York 14627, USA
Mol Cell Biol 19:1518-25. 1999..Distinct pools of Rrp43p may interact both with the exosome and with Nip7p, possibly both in the nucleus and in the cytoplasm, to catalyze analogous reactions in the multistep process of 60S ribosome biogenesis and mRNA turnover...
- A plant snoRNP complex containing snoRNAs, fibrillarin, and nucleolin-like proteins is competent for both rRNA gene binding and pre-rRNA processing in vitroJulio Saez-Vasquez
Laboratoire Génome et Développement des Plantes, UMR CNRS IRD 5096, Universite de Perpignan, France
Mol Cell Biol 24:7284-97. 2004..Based on this it is proposed that NF D is a processing complex that assembles on the rDNA prior to its interaction with the nascent pre-rRNA...
- Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNAMarlene Oeffinger
Rockefeller University, New York, NY 10065, USA
Mol Cell 36:768-81. 2009..We show that Rrp17p binds late pre-60S ribosomes, accompanying them from the nucleolus to the nuclear periphery, and provide evidence for physical and functional links between late 60S subunit processing and export...
- High-definition macromolecular composition of yeast RNA-processing complexesNevan J Krogan
Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario M5G 1L6, Canada
Mol Cell 13:225-39. 2004..This study provides a high-resolution overview of the modular topology of noncoding RNA-processing machinery...
- A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesisFrançois Dragon
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520 8040, USA
Nature 417:967-70. 2002..We have termed this large RNP the small subunit (SSU) processome...
- Homologs of small nucleolar RNAs in ArchaeaA D Omer
Department of Biochemistry and Molecular Biology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada
Science 288:517-22. 2000..snoRNA-based rRNA processing was therefore probably present in the last common ancestor of Archaea and Eukarya, predating the evolution of a morphologically distinct nucleolus...
- Cold-temperature induction of Escherichia coli polynucleotide phosphorylase occurs by reversal of its autoregulationR K Beran
1602 Molecular Science, Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA
Mol Microbiol 39:112-25. 2001..Together with other evidence, these results suggest that mRNA is generally stabilized upon a shift to cold temperatures, but that a CSR mRNA-specific decay process is initiated during adaptation...
- The stability of tristetraprolin mRNA is regulated by mitogen-activated protein kinase p38 and by tristetraprolin itselfCarmen R Tchen
Kennedy Institute of Rheumatology Division, Imperial College London, 1 Aspenlea Road, Hammersmith, London W6 8LH, United Kingdom
J Biol Chem 279:32393-400. 2004..We also demonstrate that TTP is able to bind to its own 3'-untranslated region and negatively regulate its own expression, forming a feedback loop to limit expression levels...
- P bodies: at the crossroads of post-transcriptional pathwaysAna Eulalio
Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
Nat Rev Mol Cell Biol 8:9-22. 2007....
- Arabidopsis HEN1: a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistanceStéphanie Boutet
Laboratoire de Biologie Cellulaire, INRA, 78026 Versailles Cedex, France
Curr Biol 13:843-8. 2003....
- Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1Irina Chernyakov
Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
Genes Dev 22:1369-80. 2008..The RTD pathway is the first to be implicated in the turnover of mature RNA species from the class of stable RNAs. These results and the results of others demonstrate that tRNA, like mRNA, is subject to multiple quality control steps...
- RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancerM R Culbertson
Laboratory of Genetics, R M Bock Laboratories, University of Wisconsin, Madison 53706, USA
Trends Genet 15:74-80. 1999..The NMD pathway has a direct impact on hundreds of genetic disorders in the human population, where about a quarter of all known mutations are predicted to trigger NMD...
- Degradation of RNA in bacteria: comparison of mRNA and stable RNAMurray P Deutscher
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, PO Box 016129, Miami, FL 33101 6129, USA
Nucleic Acids Res 34:659-66. 2006..Rather, only the specificity of the RNase and the accessibility of the substrate determine whether or not a particular RNA will be acted upon...
- Kinetics of the processing of the precursor to 4.5 S RNA, a naturally occurring substrate for RNase P from Escherichia coliK A Peck-Miller
Department of Biology, Yale University, New Haven, CT 06520
J Mol Biol 221:1-5. 1991..5S, is cleaved with greater efficiency than the parent molecule. The 3'-terminal CCC sequence of p4.5 S may be as important for cleavage of this substrate as the 3'-terminal CCA sequence is for cleavage of tRNA precursors...
- SnoRNP biogenesis meets Pre-mRNA splicingTamas Kiss
Laboratoire de Biologie Moleculaire Eucaryote du CNRS, 118 route de Narbonne, 31062 Toulouse, France
Mol Cell 23:775-6. 2006..In the September 1 issue of Molecular Cell, Hirose et al. (2006) demonstrate that a spliceosomal intron binding protein, IBP160, couples box C/D snoRNA processing with pre-mRNA splicing in the C1 splicing complex...
- A human, ATP-independent, RISC assembly machine fueled by pre-miRNAElisavet Maniataki
Department of Pathology, Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
Genes Dev 19:2979-90. 2005..We suggest that the miRLC couples the processing of the pre-miRNA substrate to the unwinding of the product and that after loading of the mature miRNA to Ago2, the miRLC disassembles and the miRNP is released...
- Maturation of 23S ribosomal RNA requires the exoribonuclease RNase TZ Li
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101, USA
RNA 5:139-46. 1999..coli, is able to efficiently remove nucleotides close to the double-stranded stem generated by the pairing of the 5' and 3' termini of most stable RNAs...
- A general two-metal-ion mechanism for catalytic RNAT A Steitz
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511
Proc Natl Acad Sci U S A 90:6498-502. 1993..The role of the RNA is to position the two catalytic metal ions and properly orient the substrates via three specific binding sites...
- Defining the enzyme binding domain of a ribonuclease III processing signal. Ethylation interference and hydroxyl radical footprinting using catalytically inactive RNase III mutantsH Li
Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
EMBO J 15:1421-33. 1996..1[WC-L] RNA, and dsRNA in general, while catalyzing only single cleavage of R1.1 RNA and related substrates in which the scissle bond is within an asymmetric internal loop...
- Role and dynamics of the ribosomal protein P0 and its related trans-acting factor Mrt4 during ribosome assembly in Saccharomyces cerevisiaeMaría Rodríguez-Mateos
Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas and Universidad Autonoma de Madrid, Cantoblanco E 28049 Madrid, Spain
Nucleic Acids Res 37:7519-32. 2009..We conclude that Mrt4 controls the position and timing of P0 assembly. In turn, P0 is required for the release of Mrt4 and exchanges with this factor at the cytoplasm. Our results also suggest other P0 assembly alternatives...
- RNase PH is essential for tRNA processing and viability in RNase-deficient Escherichia coli cellsK O Kelly
Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305
J Biol Chem 267:16015-8. 1992..These data indicate that RNase PH can be an essential enzyme for the processing of tRNA precursors...
- Poly(A) tail length control in Saccharomyces cerevisiae occurs by message-specific deadenylationC E Brown
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
Mol Cell Biol 18:6548-59. 1998..The ability to control the length of the poly(A) tail for newly expressed mRNAs has the potential to be an important posttranscriptional regulatory step in gene expression...
- Principles and effects of microRNA-mediated post-transcriptional gene regulationB M Engels
Division of Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK
Oncogene 25:6163-9. 2006....
- The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleasesP Mitchell
European Molecular Biology Laboratory, Heidelberg, Germany
Cell 91:457-66. 1997..8S rRNA. Human Rrp4p is found in a comparably sized complex, and expression of the hRRP4 gene in yeast complements the rrp4-1 mutation. We conclude that the exosome constitutes a highly conserved eukaryotic RNA processing complex...
- A stepwise model for double-stranded RNA processing by ribonuclease IIIJianhua Gan
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
Mol Microbiol 67:143-54. 2008..Together, the crystal structures and the models suggest a stepwise mechanism for RNase III to execute the phosphoryl transfer reaction...
- Structural Studies of RNA Maturation and ProcessingChristopher D Lima; Fiscal Year: 2013..Pathways and enzymes that regulate the abundance, lifetime, and processing of RNA have been associated with human diseases that include neurodegenerative disorders, cancer, and inflammation. ..
- Genomic analysis of RNA binding protein target specificityJEREMY ROBERT SANFORD; Fiscal Year: 2010..2) Determine the genomic landscape of cis-acting RNA elements recognized by SR proteins. (3) Functionally validate SR protein cis-acting RNA elements. ..
- RNA SPLICING IN ARCHAEARamesh Gupta; Fiscal Year: 2012..Thus, the overall understanding of these processes in archaeal systems will help us understand similar processes in human systems under normal conditions, and the changes that may occur under diseased conditions. ..
- RNA caps and meiotic pre-mRNA splicingBeate Schwer; Fiscal Year: 2013..We expect to gain new and general insights to the question of how splice site choice can be modulated by components of the basal splicing machinery in response to distinct features in the pre-mRNA. ! ..
- NUCLEIC ACIDS AND NEUCLEOPROTEINSERNEST GEIDUSCHEK; Fiscal Year: 2007....
- BRANCHED SUGAR NUCLEOSIDES AS ANTICANCER AGENTSAndre Rosowsky; Fiscal Year: 1980....
- Proteins that Bind RNAROBERT KRUG; Fiscal Year: 2001....
- POSTTRANSCRIPTIONAL GENE REGULATION IN T CELL ACTIVATIONJACK KEENE; Fiscal Year: 2004..In summary, HuR will be examined for RNA recognition specificity and interactions, which govern its effects on the stability or translation of cytokine and ERG mRNAs during T cell activation. ..
- Regulation of Lung Epithelial Fibrinolysis by UrokinaseSreerama Shetty; Fiscal Year: 2005..This information could hasten the development of new, mechanism-based interventions for lung disorders, including acute lung injury or lung neoplasia. ..
- CONTROL OF FIBRINOLYTIC PATHWAYS IN LUNG DISEASESreerama Shetty; Fiscal Year: 2004..abstract_text> ..
- Aminoacyl-tRNA synthetases in ArchaeaYa Ming Hou; Fiscal Year: 2006..jannaschii to gain a broader insight into its aminoacyl-tRNA synthetases as a basis for understanding the origin and evolution of contemporary mRNA-encoded protein synthesis in extant organisms. [unreadable] [unreadable]..
- Post-transcriptional regulation of TNF-alpha productionPaul Anderson; Fiscal Year: 2005..These studies might also identify molecular targets for the development of orally available TNFa blockers that can be used to treat patients with rheumatoid arthritis. ..
- RNA Transport Within the NucleusThoru Pederson; Fiscal Year: 2005..Another aspect of the project will address the intranuclear traffic of these RNAs in relation to proteins known to be involved in nuclear import or RNA export, i.e. the Ran-GTPase and TAP. ..
- Biogenesis of Eukaryotic RibosomesSUSAN GERBI; Fiscal Year: 2004..The proposed studies should elucidate the importance of dynamic RNA-RNA interactions during ribosome biogenesis, a fundamental process essential for cell growth and viability. ..
- Molecular Mechanism of HIV-1 Vif FunctionBRYAN CULLEN; Fiscal Year: 2007..Together, this wide-ranging series of experiments should lead to considerable insight into how HIV-1 Vif functions and potentially suggest approaches to inhibit Vif function. ..
- Maintenance of the tRNA 3' endYa Ming Hou; Fiscal Year: 2008..Because of the importance of CCA in maintenance of cellular life, the proposed studies will have a strong impact on human health and diseases. ..
- Regulation of human microRNA biosynthesis and activityBRYAN CULLEN; Fiscal Year: 2007..Finally, in specific aim 5, we seek to extend our exciting initial data demonstrating inhibition of miRNA function by the adenovirus VA1 non-coding RNA by trying to define the underlying mechanism. ..
- Dynamic Regulation of y-Globin mRNA StabilityJ Russell; Fiscal Year: 2004....
- Effect of APOBEC3G on retroviruses and retrotransposonsBRYAN CULLEN; Fiscal Year: 2009..abstract_text> ..
- RNA PROCESSING AND RIBONUCLEOPROTEINThoru Pederson; Fiscal Year: 2001..The same studies should provide information on sequences required for nuclear export. These two inter-related projects test the hypothesis that the nucleolus is a more pluri-functional organelle than previously thought. ..
- Gene Amplification-Sciarid DNA PuffsSUSAN GERBI; Fiscal Year: 2006..Information gained from the unique Sciara system should behelpful in understanding the mechanisms used by metazoa to specify an origin and to regulate its activation. ..
- HIV-1 TROPISM AND CHEMOKINE RECEPTORSBRYAN CULLEN; Fiscal Year: 2002..The final specific aim focusses on the promoter regions of CCR5, the identification of transcription factors that may interact with the promoter, and the use of antisense strategies to block CCR5 expression. ..
- Characterization of mammalian stress granulesPaul J Anderson; Fiscal Year: 2010..These studies have implications for our understanding of stress-regulated pathological conditions, including Alzheimer's disease, diabetes and cancer. ..
- REGULATION OF GENE EXPRESSION BY NO/CGMPRENATE PILZ; Fiscal Year: 2002....
- ROLE OF PSGL 1 IN NATURAL KILLER CELL FUNCTIONPaul Anderson; Fiscal Year: 2003..Consequently, the overall goal of the proposed experiments is to determine how PEN5:L-selectin interactions influence NK cell function. ..
- The role of FAST in immune-mediated inflammatory diseasePaul Anderson; Fiscal Year: 2008..Finally, we will generate transgenic mice that overexpress FAST and mutant mice that lack FAST to determine its importance in the initiation and/or propagation of immune-mediated inflammatory disease. ..
- FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIFYa Ming Hou; Fiscal Year: 2005..coli CysRS for its ability to distinguish cysteine from serine. These studies shall shed new light on the molecular interactions responsible for the accurate translation of the genetic code. ..
- NUTRITIONAL COPPER STATUS AND THE NERVOUS SYSTEMJOSEPH ROBERT PROHASKA; Fiscal Year: 2010..AIM 4: We will test the hypothesis that limitation in Cu-dependent ferroxidases lead to lower brain iron and are responsible by comparing rats reared on an iron-fortified diet to restore brain iron. ..
- Role of PKR in Growth RegulationGlen Barber; Fiscal Year: 2008..We propose that these aims will lend insight into mechanisms of cell signaling, tumorigenesis and interferon-mediated actions in the cell. ..
- Targeted pre-mRNA modification and gene silencingYi Tao Yu; Fiscal Year: 2007..We believe that our novel method will prove effective and useful in mammalian systems as well as in yeast. [unreadable] [unreadable] [unreadable]..
- A Study of Site-Specifically Modified RNAsChristine Chow; Fiscal Year: 2006..abstract_text> ..
- Analyzing respiration-dependent control of cell fate in S. cerevisiaeAshwini Jambhekar; Fiscal Year: 2007..This work will define how cells choose between growth and gametogenesis. [unreadable] [unreadable] [unreadable]..
- The Functional Analysis of the Coactivator CARM1Mark Bedford; Fiscal Year: 2007..abstract_text> ..
- Complement mediated lysis resistance genes of LeishmaniaJEFFREY BEETHAM; Fiscal Year: 2008..abstract_text> ..
- A Nucleosomal Biosensor for Identification and Isolation of Nuclear Hormone RecepRichard Steinman; Fiscal Year: 2008..This biosensor should be more rapid and versatile than cell-based reporter gene assays and more informative than assays that measure only ligand/receptor binding. [unreadable] [unreadable] [unreadable]..
- GenMAPP-CS, a dynamic resource pathway analysisBruce R Conklin; Fiscal Year: 2010..The challenge of analyzing these massive and complex datasets is a major force driving the development of GenMAPP-CS. ..
- NICOTINE EFFECTS ON THE ADRENAL MEDULLA AND BRAINARNOLD TANK; Fiscal Year: 2009..3) To test whether PCBP and the polyC-rich sequence to which it binds in the 3'UTR of TH mRNA participate in the stabilization of TH mRNA in response to the activation of signaling pathways that mediate the response to nicotine. ..
- Understanding the Mechanism of RNA Interference (RNAi)Phillip Zamore; Fiscal Year: 2009....
- microRNA Uncoupling of Protein and Transcript Expression in Liver RegenerationCLIFFORD JOHN STEER; Fiscal Year: 2010..Ultimately, the results may provide the basis for novel therapies designed to promote liver regeneration and accelerate recovery from disease states. ..
- Regulation of translation initiation in eukaryotesVincent P Mauro; Fiscal Year: 2010..g. for the production of therapeutic proteins, and new avenues for analyzing the potential contributions of mis-regulation to disease. ..
- Comparative Evolutionary Genomics and Infectious DiseaseJane Carlton; Fiscal Year: 2010....
- Molecular Mechanisms of Portal HypertensionVijay Shah; Fiscal Year: 2007..abstract_text> ..
- In Vitro Reconstitution of Alpha Fetroprotein RegulationMichelle Barton; Fiscal Year: 2007..To test this hypothesis, we will assemble AFP gene templates into developmentally repressed, solid-phase nuclei and analyze chromatin structure and transcription function before and after DNA replication. ..
- Genetic Analysis of Heavy Metals NeurotoxicityFen Biao Gao; Fiscal Year: 2005....
- Regulation of Expression of Secreted Flt-1WILLIAM HUCKLE; Fiscal Year: 2005..abstract_text> ..
- Gordon Research Conference on MyogenesisSTEPHEN TAPSCOTT; Fiscal Year: 2004..abstract_text> ..
- The Role of mRNA Editing in B Cell DevelopmentHarold Smith; Fiscal Year: 2004..abstract_text> ..
- RECONSTITUTION OF GENE REGULATION IN LIVER REGENERATIONMichelle Barton; Fiscal Year: 2003..The long-term goal of these studies is to learn how regenerative organs, such as the liver are capable of redirecting their genetic programs in response to traumatic injury. ..
- IRON TRANSPORT IN MAMMALIAN CELLSPhilip Aisen; Fiscal Year: 2003..Project 5: Structure, function and dynamics in transferrin investigates the molecular mechanisms through which the uptake and release of iron and other metals by transferrin are modulated by physiological factors. ..
- REG OF NITRIC OXIDE PRODUCT IN THE HEPATIC CIRCULATIONVijay Shah; Fiscal Year: 2002..Defining the mechanisms that regulate nitric oxide production in SEC may lead to therapeutic options whereby NO activity may be pharmacologically or genetically modulated and benefit patients with cirrhosis and portal hypertension. ..
- NEUROD AND NEUROGENESISSTEPHEN TAPSCOTT; Fiscal Year: 2001..Th health relatedness of this work is that the ability to generate neurons and neuroendocrine cells will be critical for the treatment of numerous human diseases. ..
- Attenuation of mucous cell metaplasia by RadMary Chang; Fiscal Year: 2006..In summary, we have proposed a comprehensive approach utilizing combined in vitro, cell, and in vivo methods to test our hypothesis. ..
- The Role of DGAT1 In Muscle LipotoxicityYi Hao Yu; Fiscal Year: 2006..As a result, we may be able to identify new molecular target(s) for future drug therapy aimed at reducing lipotoxicity ..
- MAP kinase signal specificity in the C. elegans germlineValerie Reinke; Fiscal Year: 2007..Together these experiments should build toward a comprehensive understanding of the mechanisms used to produce a specific cell fate in response to a generally used signaling pathway. ..
- EXPRESSION AND STRUCTURE OF THE CALCIUM SENSING RECEPTORRajiv Kumar; Fiscal Year: 2007..Furthermore, this information will serve as the basis for more extensive experiments involving the structure of the full-length receptor. [unreadable] [unreadable]..
- Control of Spemann's organizerMichael Sheets; Fiscal Year: 2007..We will address our view by examining the role(s) of FGF signaling pathway(s) within the organizer and the transcription of chordin as they relate to the ultimate inducing functions of Spemann's organizer. [unreadable] [unreadable]..
- Small Molecule Regulators of Arginine MethyltransferasesMark Bedford; Fiscal Year: 2007..Most importantly, the PRMTs are a newly identified family of enzymes that have not yet been tapped as "drugable" targets. ..
- Isotopically Labeled Nucleotides for Biomolecular NMRJames Williamson; Fiscal Year: 2007..In order to make these reagents widely available to the structural biology community, we propose to optimize and scale up production of these reagents for commercial distribution. ..
- IRON BINDING PROTEINS AND CONTROL OF IRON METABOLISMPhilip Aisen; Fiscal Year: 2001..Understanding the fundamental chemistry of iron transport, storage and utilization will provide the rational basis for managing the multitude of diseases where aberrations of iron metabolism contribute to cellular injury and death. ..
- Genetic and Biochemical Analysis of SID-1 and SID-2Craig Hunter; Fiscal Year: 2007..Although RNAi has been shown to be effective, a major obstacle remains delivery of dsRNA into human cells both in culture and in vivo. ..
- Assemply of the 30S Ribosomal SubunitJames Williamson; Fiscal Year: 2007..This will allow us to monitor the 30S assembly from a global perspective. [unreadable] [unreadable]..
- MOLECULAR MECHANISMS OF PML MEDIATED GROWTH CONTROLKATHERINE BORDEN; Fiscal Year: 2005....
- Molecul Biology of Intestinal Lipid Transport/MetabolismNICHOLAS DAVIDSON; Fiscal Year: 2003..abstract_text> ..
- Nucleic Acid Gordon Research ConferenceANNA PYLE; Fiscal Year: 2004..Poster sessions and optional recreational activities will stimulate informal interactions among participants. ..
- Immunogenicity of Hepatitis C Virus (HCV) like particlesGlen Barber; Fiscal Year: 2007..abstract_text> ..
- REGULATIOIN OF RENAL PHOSPHATE EXCRETION AND VITAMIN D METABOLISM BY FGF 7Rajiv Kumar; Fiscal Year: 2010..The interaction between the FGF 7 and other PTNs will be clarified. Such information will significantly enhance our knowledge of mineralization processes and Pi homeostasis. ..
- Innate Intracellular MechanismsGlen N Barber; Fiscal Year: 2010..The importance of STING in innate signaling will be determined. Understanding how these pathways function has significant impact on understanding pathogenesis and for developing novel therapeutics and vaccines to combat disease. ..
- TH GENE REGULATION IN HEALTHY AND LESIONED MIDBRAINARNOLD TANK; Fiscal Year: 2003..These studies will shed light on the molecular mechanisms regulating the TH gene in the midbrain and may lead to new therapeutic strategies for the treatment of Parkinson's disease. ..
- Receptor-Signaling Protein Interactions in Real TimeSidney Pestka; Fiscal Year: 2007..Further application of this technology should lead to new rapid methods for high throughput screening and for identifying protein-protein interactions in live cells in real-time. ..