cytokinesis

Summary

Summary: The process by which the CYTOPLASM of a cell is divided.

Top Publications

  1. doi Molecular control of animal cell cytokinesis
    Juan Pablo Fededa
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich, CH 8093 Zurich, Switzerland
    Nat Cell Biol 14:440-7. 2012
  2. ncbi Counting cytokinesis proteins globally and locally in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Science 310:310-4. 2005
  3. pmc Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes
    Shao Chen Sun
    Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea
    PLoS ONE 6:e18392. 2011
  4. doi Aurora B-mediated abscission checkpoint protects against tetraploidization
    Patrick Steigemann
    Institute of Biochemistry, Swiss Institute of Technology Zurich, ETHZ, Zurich, Switzerland
    Cell 136:473-84. 2009
  5. pmc Mechanics of cytokinesis in eukaryotes
    Thomas D Pollard
    Department of Molecular Cellular, Yale University, PO Box 208103, New Haven, CT 06520 8103, USA
    Curr Opin Cell Biol 22:50-6. 2010
  6. ncbi Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery
    Jez G Carlton
    Department of Infectious Diseases, King s College London School of Medicine, London, UK
    Science 316:1908-12. 2007
  7. doi Polo on the Rise-from Mitotic Entry to Cytokinesis with Plk1
    Mark Petronczki
    Cell Division and Aneuploidy Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, United Kingdom
    Dev Cell 14:646-59. 2008
  8. pmc Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia Unit, DKFZ ZMBH Alliance, German Cancer Research Center, 69120 Heidelberg, Germany
    Genes Dev 25:875-88. 2011
  9. pmc Citron kinase controls abscission through RhoA and anillin
    Marta Gai
    Department of Genetics, Biology, and Biochemistry, Molecular Biotechnology Center, University of Turin, 10126 Turin, Italy
    Mol Biol Cell 22:3768-78. 2011
  10. pmc Stabilization of the actomyosin ring enables spermatocyte cytokinesis in Drosophila
    Philip Goldbach
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, M5G 1L7, Canada
    Mol Biol Cell 21:1482-93. 2010

Detail Information

Publications342 found, 100 shown here

  1. doi Molecular control of animal cell cytokinesis
    Juan Pablo Fededa
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich, CH 8093 Zurich, Switzerland
    Nat Cell Biol 14:440-7. 2012
    b>Cytokinesis is the process by which mitotic cells physically split in two following chromosome segregation. Dividing animal cells first ingress a cytokinetic furrow and then separate the plasma membrane by abscission...
  2. ncbi Counting cytokinesis proteins globally and locally in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Science 310:310-4. 2005
    ..Proteins concentrated up to 100 times in contractile rings and 7500 times in spindle pole bodies at certain times in the cell cycle. This approach can be used to measure the global and local concentrations of any fusion protein...
  3. pmc Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes
    Shao Chen Sun
    Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea
    PLoS ONE 6:e18392. 2011
    ..included the failure of asymmetric division, spindle migration, and the formation and completion of oocyte cytokinesis. The formation of the actin cap and cortical granule-free domain (CGFD) was also disrupted, which further ..
  4. doi Aurora B-mediated abscission checkpoint protects against tetraploidization
    Patrick Steigemann
    Institute of Biochemistry, Swiss Institute of Technology Zurich, ETHZ, Zurich, Switzerland
    Cell 136:473-84. 2009
    Genomic abnormalities are often seen in tumor cells, and tetraploidization, which results from failures during cytokinesis, is presumed to be an early step in cancer formation...
  5. pmc Mechanics of cytokinesis in eukaryotes
    Thomas D Pollard
    Department of Molecular Cellular, Yale University, PO Box 208103, New Haven, CT 06520 8103, USA
    Curr Opin Cell Biol 22:50-6. 2010
    Research on eukaryotic cytokinesis using advantageous model systems is rapidly advancing our understanding of most aspects of the process. Cytokinesis is very complicated with more than 100 proteins participating...
  6. ncbi Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery
    Jez G Carlton
    Department of Infectious Diseases, King s College London School of Medicine, London, UK
    Science 316:1908-12. 2007
    During cytokinesis, as dividing animal cells pull apart into two daughter cells, the final stage, termed abscission, requires breakage of the midbody, a thin membranous stalk connecting the daughter cells...
  7. doi Polo on the Rise-from Mitotic Entry to Cytokinesis with Plk1
    Mark Petronczki
    Cell Division and Aneuploidy Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, United Kingdom
    Dev Cell 14:646-59. 2008
    ..In particular, we highlight new work on the role of Plk1 in cytokinesis in human cells...
  8. pmc Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia Unit, DKFZ ZMBH Alliance, German Cancer Research Center, 69120 Heidelberg, Germany
    Genes Dev 25:875-88. 2011
    Spatial and timely coordination of cytokinesis is crucial for the maintenance of organelle inheritance and genome integrity...
  9. pmc Citron kinase controls abscission through RhoA and anillin
    Marta Gai
    Department of Genetics, Biology, and Biochemistry, Molecular Biotechnology Center, University of Turin, 10126 Turin, Italy
    Mol Biol Cell 22:3768-78. 2011
    The small GTPase RhoA plays a crucial role in the different stages of cytokinesis, including contractile ring formation, cleavage furrow ingression, and midbody abscission...
  10. pmc Stabilization of the actomyosin ring enables spermatocyte cytokinesis in Drosophila
    Philip Goldbach
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, M5G 1L7, Canada
    Mol Biol Cell 21:1482-93. 2010
    The scaffolding protein anillin is required for completion of cytokinesis. Anillin binds filamentous (F) actin, nonmuscle myosin II, and septins and in cell culture models has been shown to restrict actomyosin contractility to the ..
  11. ncbi The (1,3)beta-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
    Juan Carlos G Cortés
    Instituto de Microbiologia Bioquimica, Consejo Superior de Investigaciones Científicas CSIC Universidad de Salamanca, 37007 Salamanca, Spain
    Mol Microbiol 65:201-17. 2007
    b>Cytokinesis is a crucial event in the cell cycle of all living cells...
  12. ncbi Cytokinesis-block micronucleus cytome assay
    Michael Fenech
    Genome Health Nutrigenomics Laboratory, CSIRO Human Nutrition, Food Science Australia, PO Box 10041, Adelaide 5000, South Australia, Australia
    Nat Protoc 2:1084-104. 2007
    The cytokinesis-block micronucleus cytome assay is a comprehensive system for measuring DNA damage, cytostasis and cytotoxicity...
  13. pmc Assembly and architecture of precursor nodes during fission yeast cytokinesis
    Damien Laporte
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    J Cell Biol 192:1005-21. 2011
    The contractile ring is essential for cytokinesis in most fungal and animal cells. In fission yeast, cytokinesis nodes are precursors of the contractile ring and mark the future cleavage site...
  14. pmc Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis
    Eiji Morita
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132 3201, USA
    EMBO J 26:4215-27. 2007
    ..Proteomic analyses revealed that ALIX and TSG101/ESCRT-I also bind a series of proteins involved in cytokinesis, including CEP55, CD2AP, ROCK1, and IQGAP1...
  15. pmc Cell polarization and cytokinesis in budding yeast
    Erfei Bi
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6058, USA
    Genetics 191:347-87. 2012
    Asymmetric cell division, which includes cell polarization and cytokinesis, is essential for generating cell diversity during development...
  16. pmc ESCRT-III governs the Aurora B-mediated abscission checkpoint through CHMP4C
    Jeremy G Carlton
    Department of Infectious Diseases, King s College London School of Medicine, London, UK
    Science 336:220-5. 2012
    ..This function correlated with its differential spatiotemporal distribution during late stages of cytokinesis. Accordingly, CHMP4C functioned in the Aurora B-dependent abscission checkpoint to prevent both premature ..
  17. pmc Fission yeast Nod1 is a component of cortical nodes involved in cell size control and division site placement
    Isabelle Jourdain
    Cell Regulation Laboratory, London Research Institute, Cancer Research UK, London, United Kingdom
    PLoS ONE 8:e54142. 2013
    ..Nodes are also considered to be precursors of the cytokinesis contractile actomyosin ring (CAR)...
  18. doi Septins regulate the contractility of the actomyosin ring to enable adherens junction remodeling during cytokinesis of epithelial cells
    Nabila Founounou
    CNRS, UMR 6290, Institute of Genetics and Development of Rennes, F 35043 Rennes, France
    Dev Cell 24:242-55. 2013
    How adhesive contacts with neighbors may affect epithelial cell cytokinesis is unknown. We report that in Drosophila, septins are specifically required for planar (but not orthogonal) cytokinesis...
  19. pmc Distinct roles of septins in cytokinesis: SEPT9 mediates midbody abscission
    Mathew P Estey
    Program in Cell Biology, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Cell Biol 191:741-9. 2010
    ..Depletion of SEPT2, SEPT7, and SEPT11 causes defects in the early stages of cytokinesis, ultimately resulting in binucleation...
  20. ncbi Myosin-II-dependent localization and dynamics of F-actin during cytokinesis
    Kausalya Murthy
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, 01003, USA
    Curr Biol 15:724-31. 2005
    The assembly of an F-actin- and myosin-II-containing contractile ring (CR) is required for cytokinesis in eukaryotic cells...
  21. pmc KIF4 regulates midzone length during cytokinesis
    Chi Kuo Hu
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 21:815-24. 2011
    Midzones, also called central spindles, are an array of antiparallel microtubules that form during cytokinesis between the separated chromosomes...
  22. pmc Morphogenesis and the cell cycle
    Audrey S Howell
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genetics 190:51-77. 2012
    ..provided foundational paradigms for the mechanisms of polarity establishment, cytoskeletal organization, and cytokinesis. Here we review our current understanding of how these morphogenetic events occur and how they are controlled ..
  23. doi Contractile-ring assembly in fission yeast cytokinesis: Recent advances and new perspectives
    I Ju Lee
    Department of Molecular Genetics, The Ohio State University, Columbus, Ohio, USA
    Cytoskeleton (Hoboken) 69:751-63. 2012
    The fission yeast Schizosaccharomyces pombe is an excellent model organism to study cytokinesis. Here, we review recent advances on contractile-ring assembly in fission yeast...
  24. pmc Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis
    Chi Kuo Hu
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Biol Cell 23:2702-11. 2012
    To achieve mitosis and cytokinesis, microtubules must assemble into distinct structures at different stages of cell division-mitotic spindles to segregate the chromosomes before anaphase and midzones to keep sister genomes apart and ..
  25. doi Cytokinesis in trypanosomes
    Helen Farr
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
    Cytoskeleton (Hoboken) 69:931-41. 2012
    b>Cytokinesis is a crucial step in the cell division cycle whereby the cell membrane and underlying cortex is remodelled and drawn together to create two new daughter cells...
  26. pmc Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast
    Younghoon Oh
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Biol Cell 23:2445-56. 2012
    How cell cycle machinery regulates extracellular matrix (ECM) remodeling during cytokinesis remains poorly understood...
  27. pmc Nonmuscle myosin II exerts tension but does not translocate actin in vertebrate cytokinesis
    Xuefei Ma
    Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 109:4509-14. 2012
    During vertebrate cytokinesis it is thought that contractile ring constriction is driven by nonmuscle myosin II (NM II) translocation of antiparallel actin filaments...
  28. pmc Cytokinesis and the contractile ring in fission yeast: towards a systems-level understanding
    Mark Bathe
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Trends Microbiol 18:38-45. 2010
    b>Cytokinesis, the final stage of the cell division cycle, requires the proper placement, assembly and contraction of an actomyosin-based contractile ring...
  29. pmc KIF14 and citron kinase act together to promote efficient cytokinesis
    Ulrike Gruneberg
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Cell Biol 172:363-72. 2006
    Multiple mitotic kinesins and microtubule-associated proteins (MAPs) act in concert to direct cytokinesis (Glotzer, M. 2005. Science. 307:1735-1739)...
  30. pmc Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference
    Changjun Zhu
    The Burnham Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 16:3187-99. 2005
    ..Our results indicate that at least 12 kinesins are involved in mitosis and cytokinesis. Eg5 (a member of the kinesin-5 family), Kif2A (a member of the kinesin-13 family), and KifC1 (a member of the ..
  31. doi Cytokinetic abscission: cellular dynamics at the midbody
    Patrick Steigemann
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich ETHZ, Schafmattstr 18, CH 8093 Zurich, Switzerland
    Trends Cell Biol 19:606-16. 2009
    ..midbody is one of the most prominent structures in dividing animal cells, yet its function in the completion of cytokinesis by abscission remains largely unknown...
  32. pmc Understanding cytokinesis: lessons from fission yeast
    Thomas D Pollard
    Thomas D Pollard is at the Department of Molecular Cellular and Developmental Biology, and the Departments of Molecular Biophysics and Biochemistry and of Cell Biology, Yale University, PO Box 208103, New Haven, Connecticut 06520 8103, USA
    Nat Rev Mol Cell Biol 11:149-55. 2010
    ..filaments and myosin II produces the force to constrict the cleavage furrow of animal cells, the complexity of cytokinesis has slowed progress in understanding the mechanism...
  33. doi Polar actomyosin contractility destabilizes the position of the cytokinetic furrow
    Jakub Sedzinski
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Nature 476:462-6. 2011
    b>Cytokinesis, the physical separation of daughter cells at the end of mitosis, requires precise regulation of the mechanical properties of the cell periphery...
  34. doi Vesicle trafficking and membrane remodelling in cytokinesis
    Hélia Neto
    Henry Wellcome Laboratory of Cell Biology, Davidson Building, Institute for Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, U K
    Biochem J 437:13-24. 2011
    All cells complete cell division by the process of cytokinesis. At the end of mitosis, eukaryotic cells accurately mark the site of division between the replicated genetic material and assemble a contractile ring comprised of myosin II, ..
  35. doi Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast
    Alberto Sanchez-Diaz
    Cancer Research U K, Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK
    Nat Cell Biol 10:395-406. 2008
    ..we call Inn1, which associates with the contractile actomyosin ring at the end of mitosis and is needed for cytokinesis. We show that Inn1 has a C2 domain at the amino terminus of the protein that is required for ingression of the ..
  36. pmc Midbody assembly and its regulation during cytokinesis
    Chi Kuo Hu
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Biol Cell 23:1024-34. 2012
    The midbody is a transient structure that connects two daughter cells at the end of cytokinesis, with the principal function being to localize the site of abscission, which physically separates two daughter cells...
  37. ncbi Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together
    A Arockia Jeyaprakash
    Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 131:271-85. 2007
    The chromosomal passenger complex (CPC) is a key regulator of chromosome segregation and cytokinesis. CPC functions are connected to its localization...
  38. doi Cytokinesis and cancer
    Antonia P Sagona
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway
    FEBS Lett 584:2652-61. 2010
    b>Cytokinesis is the final stage of cell division during which the two daughter cells separate completely...
  39. pmc The BEG (PP2A-B55/ENSA/Greatwall) pathway ensures cytokinesis follows chromosome separation
    Michael J Cundell
    University of Oxford, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
    Mol Cell 52:393-405. 2013
    b>Cytokinesis follows separase activation and chromosome segregation...
  40. pmc The roles of fission yeast ase1 in mitotic cell division, meiotic nuclear oscillation, and cytokinesis checkpoint signaling
    Akira Yamashita
    Molecular Genetics Research Laboratory, Graduate School of Science, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Mol Biol Cell 16:1378-95. 2005
    ..a conserved microtubule-associated protein family that is implicated in central spindle formation and cytokinesis. Here we characterize a role for fission yeast Ase1...
  41. pmc SNARE complexes of different composition jointly mediate membrane fusion in Arabidopsis cytokinesis
    Farid El Kasmi
    Developmental Genetics, Center for Plant Molecular Biology, University of Tubingen, 72076 Tubingen, Germany
    Mol Biol Cell 24:1593-601. 2013
    ..Although membrane fusion is required for separating daughter cells in eukaryotic cytokinesis, the SNARE complexes involved are not known...
  42. pmc DNA damage associated with mitosis and cytokinesis failure
    M T Hayashi
    Molecular and Cellular Biology Department, The Salk Institute for Biological Studies, La Jolla, CA, USA
    Oncogene 32:4593-601. 2013
    ..Here, we analyze several distinct malfunctions during mitosis and cytokinesis that lead to de novo DNA damage generation.
  43. pmc Dynamics of endosomal sorting complex required for transport (ESCRT) machinery during cytokinesis and its role in abscission
    Natalie Elia
    Cell Biology and Metabolism Program, The Eunice Kennedy Shriver National Institute of Child Health and Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:4846-51. 2011
    The final stage of cytokinesis is abscission, the cutting of the narrow membrane bridge connecting two daughter cells...
  44. pmc Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 174:391-402. 2006
    ..Their absence delays late steps in cytokinesis, including septum formation and cell separation.
  45. pmc 3D-SIM super resolution microscopy reveals a bead-like arrangement for FtsZ and the division machinery: implications for triggering cytokinesis
    Michael P Strauss
    The ithree Institute, University of Technology Sydney, Sydney, New South Wales, Australia
    PLoS Biol 10:e1001389. 2012
    ..acts as a scaffold to recruit other division proteins to this site as well as providing a contractile force for cytokinesis. To understand how FtsZ performs these functions, the in vivo architecture of the Z ring needs to be ..
  46. doi Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis
    Sergey Lekomtsev
    Cell Division and Aneuploidy Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 492:276-9. 2012
    At the end of cell division, cytokinesis splits the cytoplasm of nascent daughter cells and partitions segregated sister genomes...
  47. pmc BRCA2 localization to the midbody by filamin A regulates cep55 signaling and completion of cytokinesis
    Gourish Mondal
    Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Dev Cell 23:137-52. 2012
    ..BRCA2-deficient cells may partly result from aberrations in cell division caused by disruption of BRCA2 during cytokinesis. Here we show that BRCA2 is a component of the midbody that is recruited through an interaction with Filamin A ..
  48. doi GAS2L3, a target gene of the DREAM complex, is required for proper cytokinesis and genomic stability
    Patrick Wolter
    Biozentrum, Universitat Wurzburg, Wurzburg, Germany
    J Cell Sci 125:2393-406. 2012
    ..gene transcription and drives the expression of many gene products required for mitosis and cytokinesis. In this study, we characterized GAS2L3, which belongs to the GAS2 family of proteins with putative actin- and ..
  49. pmc Anillin-related protein Mid1p coordinates the assembly of the cytokinetic contractile ring in fission yeast
    Shambaditya Saha
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 23:3982-92. 2012
    ..We conclude that orderly assembly of the contractile ring in wild-type cells depends on Mid1p to recruit myosin-II, Rng2p, and Cdc15p to nodes and to place cytokinetic nodes around the cell equator...
  50. pmc PRC1 controls spindle polarization and recruitment of cytokinetic factors during monopolar cytokinesis
    Sanjay Shrestha
    Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA
    Mol Biol Cell 23:1196-207. 2012
    ..is a postanaphase array of microtubules that plays an essential role in organizing the signaling machinery for cytokinesis. The model by which the central spindle organizes the cytokinetic apparatus is premised on an antiparallel ..
  51. doi Cleavage furrow organization requires PIP(2)-mediated recruitment of anillin
    Jinghe Liu
    Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada
    Curr Biol 22:64-9. 2012
    ..These data demonstrate that the anillin PH domain has two functions: targeting anillin to the furrow by binding to PI(4,5)P(2) to maintain furrow organization and recruiting septins to the furrow...
  52. pmc Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis
    I Ju Lee
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    J Cell Sci 125:2973-85. 2012
    Division-site selection and contractile-ring assembly are two crucial steps in cytokinesis. In fission yeast, the anillin-like Mid1 protein specifies the division site at the cell equator by assembling cortical nodes, the precursors of ..
  53. pmc The ESCRT complexes
    James H Hurley
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD 20892, USA
    Crit Rev Biochem Mol Biol 45:463-87. 2010
    ..lysosome and the yeast vacuole; the budding of most membrane enveloped viruses; the membrane abscission step in cytokinesis; macroautophagy; and several other processes...
  54. doi Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast
    Franz Meitinger
    German Cancer Research Centre, DKFZ ZMBH Alliance, Molecular Biology of Centrosomes and Cilia Unit, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany
    J Cell Sci 123:1851-61. 2010
    The mitotic-exit network (MEN) is a signaling pathway that is essential for the coordination of mitotic exit and cytokinesis. Whereas the role of the MEN in mitotic exit is well established, the molecular mechanisms by which MEN ..
  55. doi Mechanisms of cytokinesis in budding yeast
    Carsten Wloka
    Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA
    Cytoskeleton (Hoboken) 69:710-26. 2012
    b>Cytokinesis is essential for cell proliferation in all domains of life...
  56. doi PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody
    Antonia P Sagona
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Nat Cell Biol 12:362-71. 2010
    ..uncover a function for this complex and its catalytic product phosphatidylinositol-3-phosphate (PtdIns(3)P) in cytokinesis. We show that PtdIns(3)P localizes to the midbody during cytokinesis and recruits a centrosomal protein, FYVE-..
  57. pmc Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae
    Ryuichi Nishihama
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 185:995-1012. 2009
    b>Cytokinesis requires coordination of actomyosin ring (AMR) contraction with rearrangements of the plasma membrane and extracellular matrix...
  58. pmc Anillin acts as a bifunctional linker coordinating midbody ring biogenesis during cytokinesis
    Amel Kechad
    CHU Sainte Justine Centre de Recherche, Centre de cancérologie Charles Bruneau, 3175 Chemin de la Côte Ste Catherine, Montreal, Quebec H3T 1C5, Canada
    Curr Biol 22:197-203. 2012
    Animal cell cytokinesis proceeds via constriction of an actomyosin-based contractile ring (CR) [1, 2]. Upon reaching a diameter of ~1 μm [3], a midbody ring (MR) forms to stabilize the intercellular bridge until abscission [4-6]...
  59. pmc The nuclear kinase Lsk1p positively regulates the septation initiation network and promotes the successful completion of cytokinesis in response to perturbation of the actomyosin ring in Schizosaccharomyces pombe
    Jim Karagiannis
    Laboratory of Cell Division, Temasek Life Sciences Laboratory, Singapore 117604, Singapore
    Mol Biol Cell 16:358-71. 2005
    b>Cytokinesis in fission yeast requires the function of an actomyosin-based contractile ring whose constriction is dependent on a signaling module termed the septation initiation network (SIN)...
  60. pmc Roles of formin nodes and myosin motor activity in Mid1p-dependent contractile-ring assembly during fission yeast cytokinesis
    Valerie C Coffman
    Department of Molecular Genetics, Graduate Program of Molecular, Cellular, and Developmental Biology, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 20:5195-210. 2009
    Two prevailing models have emerged to explain the mechanism of contractile-ring assembly during cytokinesis in the fission yeast Schizosaccharomyces pombe: the spot/leading cable model and the search, capture, pull, and release (SCPR) ..
  61. ncbi Formin-2 is required for spindle migration and for the late steps of cytokinesis in mouse oocytes
    Julien Dumont
    Equipe Divisions Méiotiques chez la souris, UMR7622, CNRS, Universite Pierre et Marie Curie Paris 6, Bat C, 5e, 9 Quai Saint Bernard 75005, Paris, France
    Dev Biol 301:254-65. 2007
    ..not due to a lack of cortical differentiation or central spindle formation but to a defect in the late steps of cytokinesis. Indeed, Survivin, a component of the passenger protein complex, is correctly localized on the central spindle ..
  62. ncbi Cytokinesis failure generating tetraploids promotes tumorigenesis in p53-null cells
    Takeshi Fujiwara
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 437:1043-7. 2005
    ..Here we test this idea by transiently blocking cytokinesis in p53-null (p53-/-) mouse mammary epithelial cells (MMECs), enabling the isolation of diploid and tetraploid ..
  63. ncbi Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagellar membrane formation
    Jacqueline A Davidge
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    J Cell Sci 119:3935-43. 2006
    ..Movement of the FC in the apparent absence of intraflagellar transport raises the possibility of a new form of motility within a eukaryotic flagellum...
  64. pmc The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells
    Ryota Uehara
    Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Proc Natl Acad Sci U S A 106:6998-7003. 2009
    ..Human augmin knockdown also reduces the number of central spindle MTs during anaphase and causes late-stage cytokinesis failure...
  65. ncbi SIN and the art of splitting the fission yeast cell
    Andrea Krapp
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges, Switzerland
    Curr Biol 14:R722-30. 2004
    The septation initiation network (SIN) triggers the onset of cytokinesis in the fission yeast Schizosaccharomyces pombe by promoting contraction of the medially placed F-actin ring...
  66. doi Visualization of F-actin localization and dynamics with live cell markers in Neurospora crassa
    Diego L Delgado-Alvarez
    Departamento de Microbiologia, Centro de Investigación Científica y de Educación Superior de Ensenada CICESE, Ensenada, B C, Mexico
    Fungal Genet Biol 47:573-86. 2010
    ..other eukaryotes, in a wide variety of cellular processes including cell growth, intracellular motility, and cytokinesis. We visualized F-actin organization and dynamics in living Neurospora crassa cells via confocal microscopy of ..
  67. pmc FtsZ in bacterial cytokinesis: cytoskeleton and force generator all in one
    Harold P Erickson
    Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA
    Microbiol Mol Biol Rev 74:504-28. 2010
    ..Recent efforts to develop antibacterial drugs that target FtsZ are reviewed. Finally, we discuss evidence of how FtsZ generates a constriction force: by protofilament bending into a curved conformation...
  68. doi The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins
    Camilla Raiborg
    Centre for Cancer Biomedicine, Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Nature 458:445-52. 2009
    ..Then, through a highly conserved mechanism also used in cytokinesis and viral budding, it mediates the breaking off of the cargo-containing intraluminal vesicles from the ..
  69. pmc Accelerated aging and failure to segregate damaged proteins in Sir2 mutants can be suppressed by overproducing the protein aggregation-remodeling factor Hsp104p
    Nika Erjavec
    Department of Cell and Molecular Biology, Goteborg University, 413 90 Goteborg, Sweden
    Genes Dev 21:2410-21. 2007
    ..aggregates and that these aggregates, like oxidized proteins, are retained in the progenitor cell during cytokinesis by a Sir2p-dependent process...
  70. pmc Blt1 and Mid1 provide overlapping membrane anchors to position the division plane in fission yeast
    Mercè Guzman-Vendrell
    Institut Curie, Centre de Recherche, Paris, France
    Mol Cell Biol 33:418-28. 2013
    Spatial control of cytokinesis is essential for proper cell division. The molecular mechanisms that anchor the dynamic assembly and constriction of the cytokinetic ring at the plasma membrane remain unclear...
  71. pmc MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis
    Seongju Lee
    Cell Biology Section, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 23:4347-61. 2012
    Diverse cellular processes, including multivesicular body formation, cytokinesis, and viral budding, require the sequential functions of endosomal sorting complexes required for transport (ESCRTs) 0 to III...
  72. pmc Characterization of structural and functional domains of the anillin-related protein Mid1p that contribute to cytokinesis in fission yeast
    Shambaditya Saha
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 23:3993-4007. 2012
    Fission yeast cells depend on the anillin-related protein Mid1p for reliable cytokinesis. Insolubility limits the purification of full-length Mid1p for biophysical analysis, and lack of knowledge about the structural domains of Mid1p ..
  73. pmc Bacterial cytokinesis: From Z ring to divisome
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas
    Cytoskeleton (Hoboken) 69:778-90. 2012
    Ancestral homologues of the major eukaryotic cytoskeletal families, tubulin and actin, play critical roles in cytokinesis of bacterial cells...
  74. pmc Polar opposites: Fine-tuning cytokinesis through SIN asymmetry
    Alyssa E Johnson
    Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee, USA
    Cytoskeleton (Hoboken) 69:686-99. 2012
    ..body (SPB) localized signaling cascade termed the septation initiation network (SIN) couples mitotic exit with cytokinesis. The SIN is controlled at many levels to ensure that cytokinesis is executed once per cell cycle and only after ..
  75. doi JMY is required for asymmetric division and cytokinesis in mouse oocytes
    Shao Chen Sun
    Department of Animal Sciences, Chungbuk National University, Cheongju 361 763, Korea
    Mol Hum Reprod 17:296-304. 2011
    ..Depletion of JMY by RNAi resulted in symmetric division, failure of spindle migration and cytokinesis during oocyte meiotic maturation, showing a 2-cell-like MII oocyte and TI stage arrest...
  76. pmc G protein-coupled receptors participate in cytokinesis
    Xin Zhang
    Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA
    Cytoskeleton (Hoboken) 69:810-8. 2012
    b>Cytokinesis, the last step during cell division, is a highly coordinated process that involves the relay of signals from both the outside and inside of the cell...
  77. pmc Endocytic transport and cytokinesis: from regulation of the cytoskeleton to midbody inheritance
    John A Schiel
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
    Trends Cell Biol 23:319-27. 2013
    Abscission is the last step of cytokinesis that leads to the physical separation of two daughter cells. An emerging picture is that abscission is a complex event that relies on changes in both lipid composition and cytoskeletal dynamics...
  78. doi The AP-1 μ adaptin is required for KNOLLE localization at the cell plate to mediate cytokinesis in Arabidopsis
    Ooi Kock Teh
    Department of Botany, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto, 606 8502 Japan
    Plant Cell Physiol 54:838-47. 2013
    ..mutant ap1m2 displayed deleterious growth defects, which were particularly evident in the compromised cytokinesis that was revealed by the presence of cell wall stubs in multinucleate cells...
  79. doi Cell migration or cytokinesis and proliferation?--revisiting the "go or grow" hypothesis in cancer cells in vitro
    Tamás Garay
    2nd Department of Pathology, Semmelweis University, Budapest, Hungary
    Exp Cell Res 319:3094-103. 2013
    ..Following three-day-long videomicroscopy, migration, proliferation and cytokinesis-length were quantified...
  80. pmc Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis
    Yi Hua Zhu
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210 Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH 43210 Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210
    Mol Biol Cell 24:3187-204. 2013
    b>Cytokinesis is the last step of the cell-division cycle, which requires precise spatial and temporal regulation to ensure genetic stability...
  81. pmc Genetic interactions with mutations affecting septin assembly reveal ESCRT functions in budding yeast cytokinesis
    Michael A McMurray
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    Biol Chem 392:699-712. 2011
    ..Saccharomyces cerevisiae bud neck provides new membrane and membrane-associated factors that are critical for cytokinesis. It remains unknown whether yeast plasma membrane abscission, the final step of cytokinesis, occurs ..
  82. ncbi Two kinesins are involved in the spatial control of cytokinesis in Arabidopsis thaliana
    Sabine Muller
    Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California 92093, USA
    Curr Biol 16:888-94. 2006
    ..During cytokinesis, the microtubule- and F-actin-based phragmoplast facilitates construction of a new cell wall and is guided to ..
  83. ncbi R1R2R3-Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana
    Nozomi Haga
    Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464 8601, Japan
    Development 134:1101-10. 2007
    ..MYB3R4, which encode homologs of NtmybA1 and NtmybA2, play a partially redundant role in positively regulating cytokinesis in Arabidopsis thaliana...
  84. doi AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis
    Qingning Zeng
    Department of Botany, University of British Columbia, Vancouver, Canada
    Plant J 67:895-906. 2011
    ..MAPK) cascades have been implicated in regulating various aspects of plant development, including somatic cytokinesis. The evolution of expanded plant MAPK gene families has enabled the diversification of potential MAPK cascades, ..
  85. doi Targeting of the RhoGEF Ect2 to the equatorial membrane controls cleavage furrow formation during cytokinesis
    Kuan Chung Su
    Cell Division and Aneuploidy Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Dev Cell 21:1104-15. 2011
    ..Here, we show that Ect2 concentrates at the equatorial membrane during cytokinesis in live cells...
  86. pmc Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants
    Michiko Sasabe
    Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    Proc Natl Acad Sci U S A 108:17844-9. 2011
    b>Cytokinesis in eukaryotes involves specific arrays of microtubules (MTs), which are known as the "central spindle" in animals, the "anaphase spindle" in yeasts, and the "phragmoplast" in plants...
  87. doi The dynamin inhibitors MiTMAB and OcTMAB induce cytokinesis failure and inhibit cell proliferation in human cancer cells
    Sanket Joshi
    Children s Medical Research Institute, The University of Sydney, Westmead, New South Wales, Australia
    Mol Cancer Ther 9:1995-2006. 2010
    ..protein dynamin II (dynII) participates in cell cycle progression and has roles in centrosome cohesion and cytokinesis. We have described a series of small-molecule inhibitors of dynamin [myristyl trimethyl ammonium bromides (..
  88. pmc Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis
    Olivier Hachet
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, 1066 Epalinges s Lausanne, Switzerland
    Genes Dev 22:3205-16. 2008
    In both animal cells and fungi, cytokinesis proceeds via a contractile actomyosin ring (CAR). Many CAR components and regulators are evolutionarily conserved...
  89. pmc Generation of trisomies in cancer cells by multipolar mitosis and incomplete cytokinesis
    David Gisselsson
    Department of Clinical Genetics, Lund University, University and Regional Laboratories, University Hospital, SE 221 85 Lund, Sweden
    Proc Natl Acad Sci U S A 107:20489-93. 2010
    ..SAC through tripolar cell divisions in which sister chromatid separation proceeds in a regular fashion, but cytokinesis failure nevertheless leads to an asymmetrical segregation of chromosomes into two daughter cells...
  90. pmc Rab11A-controlled assembly of the inner membrane complex is required for completion of apicomplexan cytokinesis
    Carolina Agop-Nersesian
    Department of Parasitology, Hygieneinstitut, University Hospital Heidelberg, Heidelberg, Germany
    PLoS Pathog 5:e1000270. 2009
    ..We propose a model where Rab11A-mediated vesicular traffic driven by an MTIP-Myosin motor is necessary for IMC maturation and to deliver new plasma membrane to daughter cells in order to complete cell division...
  91. doi Cytokinesis: catch and drag
    Mithilesh Mishra
    Temasek Life Sciences Laboratory, 117604 Singapore
    Curr Biol 18:R247-50. 2008
    ....
  92. pmc Actin-depolymerizing protein Adf1 is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast
    Kentaro Nakano
    Division of Biology, Department of Life Sciences, Graduate Program in Interdisciplinary Sciences, School of Arts and Sciences, University of Tokyo, Tokyo 153 8902, Japan
    Mol Biol Cell 17:1933-45. 2006
    The role of the actin-depolymerizing factor (ADF)/cofilin-family protein Adf1 in cytokinesis of fission yeast cells was studied...
  93. ncbi The actin-binding and bundling protein, EPLIN, is required for cytokinesis
    Megan Chircop
    Queensland Institute of Medical Research, Post Office Royal Brisbane Hospital, Brisbane, Queensland, Australia
    Cell Cycle 8:757-64. 2009
    b>Cytokinesis involves two phases: (1) membrane ingression followed by (2) membrane abscission...
  94. doi Aurora B-mediated phosphorylation of RASSF1A maintains proper cytokinesis by recruiting Syntaxin16 to the midzone and midbody
    Su Jung Song
    National Research Laboratory for Genomic Stability, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea
    Cancer Res 69:8540-4. 2009
    Aurora B is critically involved in ensuring proper cytokinesis and maintaining genomic stability...
  95. doi Positioning cytokinesis
    Snezhana Oliferenko
    Temasek Life Sciences Laboratory and the Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    Genes Dev 23:660-74. 2009
    b>Cytokinesis is the terminal step of the cell cycle during which a mother cell divides into daughter cells. Often, the machinery of cytokinesis is positioned in such a way that daughter cells are born roughly equal in size...
  96. doi How to scaffold the contractile ring for a safe cytokinesis - lessons from Anillin-related proteins
    Pier Paolo D'Avino
    Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
    J Cell Sci 122:1071-9. 2009
    ..To achieve a successful cytokinesis, these actomyosin filaments need to be assembled in an organized manner...
  97. ncbi MASTL is the human orthologue of Greatwall kinase that facilitates mitotic entry, anaphase and cytokinesis
    Erik Voets
    Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Cell Cycle 9:3591-601. 2010
    ..This causes chromatin to be trapped in the cleavage furrow, which may lead to the formation of 4N G(1) cells by cytokinesis failure...
  98. pmc Inhibition of Rac by the GAP activity of centralspindlin is essential for cytokinesis
    Julie C Canman
    Institute for Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Science 322:1543-6. 2008
    During cytokinesis, the guanosine triphosphatase (GTPase) RhoA orchestrates contractile ring assembly and constriction...
  99. pmc Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis
    Nolan Ko
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 18:5139-53. 2007
    ..This ring remains at the bud neck until the onset of cytokinesis, when actin is recruited to it...
  100. pmc Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release
    Jez G Carlton
    Department of Infectious Diseases, King s College London School of Medicine, London SE1 9RT, United Kingdom
    Proc Natl Acad Sci U S A 105:10541-6. 2008
    ..topologically equivalent membrane fission events, namely multivesicular body formation, the terminal stages of cytokinesis and HIV-1 release...
  101. pmc Regulation of cytokinesis by Rho GTPase flux
    Ann L Miller
    Department of Zoology, University of Wisconsin Madison, Madison, WI 53706, USA
    Nat Cell Biol 11:71-7. 2009
    In animal cells, cytokinesis is powered by a contractile ring of actin filaments (F-actin) and myosin-2. Formation of the contractile ring is dependent on the small GTPase RhoA, which is activated in a precise zone at the cell equator...

Research Grants72

  1. Functional study of C53 protein as a novel regulator of checkpoint kinases
    Honglin Li; Fiscal Year: 2012
    ..S phase, correct segregation of chromosomes during mitosis (M phase) and final separation of daughter cells in cytokinesis. Cells utilize the cell cycle checkpoints to ensure the completion of critical event of a particular phase ..
  2. Novel therapies for Fanconi anemia
    DAVID A contact WILLIAMS; Fiscal Year: 2010
    ..Indeed, we have recently found a link between DNA damage and cytokinesis failure in FA that may play a critical role in the bone marrow failure phenotype...
  3. SIGNALING THROUGH RHO GTP/GDP EXCHANGE FACTORS
    Philip B Wedegaertner; Fiscal Year: 2013
    ..localized at specific mitotic structures, including centrosomes and mitotic spindles in early mitosis and the cytokinesis cleavage furrow and midbody in late mitosis/cytokinesis...
  4. AAA ATPases linking mitochondria with microtubule processing in flies and yeast
    Karen G Hales; Fiscal Year: 2012
    ..Both paralogs seem to be required also for male meiotic cytokinesis, perhaps through effects on the meiotic spindle. A yeast ortholog exists but has not been well characterized...
  5. CYCLE REGULATION IN THE SYMBIONT SINORHIZOBIUM MELILOTI
    KATHERINE ELISABETH GIBSON; Fiscal Year: 2011
    ..further demonstrate that each HK mutant is unable to properly coordinate DNA replication initiation with cytokinesis. Thus, our preliminary data implicate HKs in cell cycle control and we will further dissect their mechanistic ..
  6. PKC/PKA Regulation in Prostate Cancer by SSeCKS/Gravin/AKAP12
    Irwin H Gelman; Fiscal Year: 2013
    ..biochemical experiments, we hypothesize the loss of SSeCKS induces hyperactive PKC*/,,which in turn induce i) cytokinesis defects through the activation of RhoA and LIMK, and through the suppression of the mitotic exit network kinase,..
  7. 2011 Cell-Cell Fusion Gordon Research Conference and Seminar
    NANCY RYAN GRAY; Fiscal Year: 2011
    ..fusion and the larger fields of membrane fusion and fission during exocytosis, endocytosis, viral entry, and cytokinesis. The cell fusion community is entering a particularly fascinating phase as the fundamental principles of fusion ..
  8. Molecular genetics of epithelium formation in C. elegans
    Stephen E Von Stetina; Fiscal Year: 2010
    ..Surprisingly, this screen identified regulators of cytokinesis, the central-spindlin components ZEN-4/kinesin and CYK-4/GAP...
  9. Specification of actomyosin function in the cell
    Matthew Lord; Fiscal Year: 2013
    ..is directed to sites of endocytosis (actin patches), how myosin-II functions at contractile rings during cytokinesis, and how myosin-V is specified for intracellular transport along actin cables...
  10. Developmental Regulation of the Cell Cycle in Drosophila
    LAURA ANNE LEE; Fiscal Year: 2013
    ..that overcome arrest exhibit severe defects in meiotic spindle assembly, chromosome segregation, and cytokinesis. Furthermore, the centriole-derived basal body is detached from the nucleus in asun postmeiotic spermatids, ..
  11. Cellular and Biochemical Studies of Myosin Assembly in Dictyostelium
    Thomas Egelhoff; Fiscal Year: 2009
    Myosin II plays fundamental roles in cytokinesis, cell migration, and cell shape changes during development...
  12. Design and Implementation of Genetically Encoded Myosin Based Force Sensors
    DANIEL PETER KIEHART; Fiscal Year: 2013
    ..with the dynamic cytoskeleton in vivo to produce forces that drive cell shape changes for morphogenesis, cytokinesis and cell locomotion is unclear...
  13. STUDIES ON OVARIAN RING CANALS IN DROSOPHILA
    Lynn Cooley; Fiscal Year: 2009
    ..Ring canals originate from arrested cytokinesis during germline proliferation, followed by stabilization of the stalled cleavage furrows with cytoskeletal ..
  14. Signaling iteractions in cytokinesis, cell wall biogenesis, and growth in fungi
    Jose R Rodriguez-Medina; Fiscal Year: 2011
    ..goals of this application are to 1- understand signaling interactions connecting myosin II functions in cytokinesis, cell wall biogenesis, and cell growth 2- understand the relation between myosin II function and cell wall ..
  15. Study of Anti-Survival signals in NF1
    CHANGYAN CHEN; Fiscal Year: 2013
    ..events, leading from the splitting of sister chromatids at anaphase onset to the completion of cell division by cytokinesis. If insults target late stages of mitosis, mitotic exit checkpoint would be activated and subsequently induce ..
  16. Studies of Lymphoma Suppression and DNA Repair
    Chunying Du; Fiscal Year: 2013
    ..Until recently, BRUCE has only been shown to be involved in apoptosis inhibition, cytokinesis, and mouse embryogenesis...
  17. REGULATION OF THE DEVELOPMENT OF TRYPANOSOMA BRUCEI
    CHING CHUNG WANG; Fiscal Year: 2012
    ..brucei have to be coordinated for a fruitful cell division, suggesting also that cytokinesis could be controlled by either of the two cycles...
  18. Regulation of Borealin Function by Mitotic Phosphorylation
    William Taylor; Fiscal Year: 2009
    ..binds to centromeres and microtubules and coordinates chromosome segregation and division of the cell during cytokinesis. We have recently identified a phosphorylated form of Borealin in mitotic cells and propose to analyze the role ..
  19. Peptidoglycan Biogenesis in Escherichia Coli
    Thomas G Bernhardt; Fiscal Year: 2013
    ..amidases) AmiA and AmiB that stimulates their activity to bring about daughter cell separation during cytokinesis. Specific Aim 3 seeks to determine how EnvC and the amidases cooperate to perform such a delicate operation ..
  20. Mechanism of Pseudomonas-mediated epithelial cell damage
    Joanne N Engel; Fiscal Year: 2010
    ..on bacterial internalization, cell migration, and host cell cytoskeleton, we have discovered that ExoT inhibits cytokinesis and induces apoptosis, in part through interfering with Crk function )...
  21. Regulation of Cytokinesis and Tumor Formation by RhoA
    ANN LOUISE MILLER; Fiscal Year: 2013
    b>Cytokinesis is the final stage of cell division where one cell is separated into two daughter cells...
  22. Identification of Novel Drug Targets For Use in Preventing Deafness Caused by NF2
    CRISTINA MARIA FERNANDEZ-VALLE; Fiscal Year: 2013
    ..Alternatively, drugs that modify actin regulatory proteins could promote cell death as a result of failed cytokinesis and mitotic spindle organization...
  23. Pathogenesis of TSC2-Related Tumors
    Raymond Yeung; Fiscal Year: 2012
    ..Since microtubules play an important role in long-distant transport, cell polarity, migration and cytokinesis, we hypothesize that mTOR may affect these processes via a microtubule-dependent mechanism...
  24. CELLULAR AND MOLECULAR MICROBIOLOGY TRAINING PROGRAM
    JACK J HAWIGER; Fiscal Year: 2010
    ..Virus-Cell Interactions;(iii) Microbial Adhesion to Cellular Receptors;(iv) Microbial Cues for Cytoskeleton, Cytokinesis and Cell Cycle;(v) Endosomal-Lysosomal Pathway of Microbial Internalization and Processing;(vi) Signal ..
  25. Roles of the Unfolded Protein Response in Cell Cycle
    MAHO R NIWA; Fiscal Year: 2013
    ..have found that: (1) functionally stressed ER is not transmitted to daughter cells, and (2) results in block in cytokinesis in S. cerevisiae...
  26. Mechanisms of megakaryocyte maturation
    Diane S Krause; Fiscal Year: 2013
    ..that the initial endomitotic cleavage event in which cells progress from 2N to 4N occurs due to failure at late cytokinesis, whereas later endomitotic events (4N to 8N, 8N to16N, etc...
  27. Regulation of cell division in Trypanosoma brucei
    Ziyin Li; Fiscal Year: 2011
    ..system in the protozoan parasite Trypanosoma brucei possesses many unusual features, among which mitosis and cytokinesis represent the most unusual aspects to be explored in more detail. Mitosis in T...
  28. Signaling pathways that mediate mammalian oocyte cortical mechanics
    Douglas N Robinson; Fiscal Year: 2013
    ..in this proposal is how does the oocyte undergo the necessary asymmetric cell divisions during meiotic cytokinesis to create the egg and the polar bodies? The answer to this fundamental question is not as simple as might be ..
  29. Regulation of cell division by mitotic kinases
    MICHAEL ALTERMAN LAMPSON; Fiscal Year: 2012
    ..At anaphase onset Aurora B redistributes to the spindle midzone and controls anaphase microtubule dynamics and cytokinesis. Changes in kinase localization and interactions with other regulatory proteins suggest varying patterns of ..
  30. IR-LAMP: Optigenetic technology to spatially manipulate protein function in vivo
    Julie C Canman; Fiscal Year: 2011
    ..sensitive mutations in the molecular machinery required for the highly spatially regulated process of cytokinesis. Cytokinesis is the last stage of cell division, when a mother cell is physically divided into two daughter ..
  31. Regulation of Cytokinesis in Fission yeast.
    Dannel McCollum; Fiscal Year: 2013
    ..The two pathways are functionally distinct: the Hippo/Lats and SIN pathways play roles in cytokinesis and mitotic exit whereas the Ndr1/2 and MOR pathways function in cell polarity...
  32. Defining the Mammalian Intercellular Bridge Interactome
    Martin M Matzuk; Fiscal Year: 2010
    ..Protein 1 (MKLP1) and Male germ cell Rac GTPase-activating protein (MgcRacGap), that are both necessary for cytokinesis and also components of the intercellular bridge;TEX14 converts these midbody proteins into stable intercellular ..
  33. Regulation of Cell Polarity During Epidermal Growth and Homeostasis
    DANELLE N DEVENPORT; Fiscal Year: 2013
    ..from the plasma membrane into cytoplasmic membrane vesicles, transported back to the plasma membrane during cytokinesis and relocalized in polarized manner...
  34. Regulation & Function of Calponin
    Jian Ping Jin; Fiscal Year: 2010
    ..We have shown that h2-calponin is rapidly degraded during cytokinesis or in cells that are under reduced tension...
  35. Chemical Genetics of Plasmodium Kinases
    Debopam Chakrabarti; Fiscal Year: 2010
    ..cell cycle of the malaria parasite, where DNA replicates more than once per cell cycle without cytokinesis. Although several homologues of CDK-like kinases have been identified in P...
  36. Assembly and Function of the Yeast Spore Wall
    Aaron M Neiman; Fiscal Year: 2013
    ..complex mediates a signaling pathway that initiates assembly of the wall only after the completion of cytokinesis. A structure-function analysis of the Gip1 protein will be used to explore the basis by which membrane closure ..
  37. The Role of an Intraflagellar Transport Protein in Cell-Cycle Control
    Christopher R Wood; Fiscal Year: 2010
    ..in IFT27 causes loss of the cilium, taken together with the fact that the presence of IFT27 is required for completion of mitosis and cytokinesis provides a possible molecular link between the cilium and diseases of cell proliferation.
  38. REGULATION OF THE DEVELOPMENT OF TRYPANOSOMA BRUCEI
    Ching Wang; Fiscal Year: 2009
    ..bruce! have to be coordinated for a fruitful cell to be fruitful cell division, suggesting also that cytokinesis could be controlled by either of the two cycles...
  39. PI(3,4,5)P3 Regulates Cell Polarity During Chemotaxis and Cytokinesis
    Christopher Janetopoulos; Fiscal Year: 2012
    ..We are using the amoeba Dictyostelium as a model system to understand the basic mechanisms that control these changes in cell shape. Many of the homologous components we are studying are mutated or lost in many human diseases. ..
  40. Role of DOCK8 in B Cell Function
    RAIF SALIM GEHA; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Deficiency in the dedicator of cytokinesis-8 (DOCK8) gene, a guanine nucleotide exchange factor, results in autosomal recessive Hyper-IgE syndrome characterized by recurrent pulmonary infections, ..
  41. Mechanisms of the Unusual Cytokinesis in Trypanosomes
    Ziyin Li; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Cytokinesis in Trypanosoma brucei, a parasitic protozoan and the causative agent of human sleeping sickness, is known to be totally different from that in its human host...
  42. FASEB SRC on Mitosis: Spindle Assembly and Function
    Claire E Walczak; Fiscal Year: 2012
    ..Kinetochore assembly and function;5. Chromosome structure and function;6. Mitosis career symposium;7. Cytokinesis and mitotic exit;8. Chromosome segregation and aneuploidy;9. Mitosis in Development...
  43. Modeling Contractile Ring Constriction in Fission Yeast
    BEN O'SHAUGHNESSY; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Cytokinesis is the process ending the cell cycle in which the mother cell cytoplasm divides into two...
  44. ALVEOLAR BASEMENT MEMBRANE/CELL INTERACTIONS IN THE LUNG
    Philip L Sannes; Fiscal Year: 2013
    ..The hypothesis to be addressed is: Following DNA synthesis and cytokinesis, exposure of the daughter AT2 cell to high levels of sulfated ECMs triggers enhanced expression of Foxa1 and ..
  45. TEMPORAL AND SPATIAL CONTROL OF BACTERIAL CYTOKINESIS
    PETRA A LEVIN; Fiscal Year: 2013
    ....
  46. HEF1 Coordination of Cell Cycle, Migration, and Invasion in Cancer
    Erica A Golemis; Fiscal Year: 2013
    ..Aim 2, we will define the role of Aurora A, Ajuba, and HDAC6 in allowing HEF1 to return to focal adhesions at cytokinesis, and the importance of interactions between HEF1, Ajuba, and HDAC6 in interphase for HEF1-dependent cell ..
  47. Defining novel pathways that arrest genetically unstable tetraploid cells
    Neil J Ganem; Fiscal Year: 2013
    ..to comprehensively identify proteins that are necessary to activate or maintain G1 cell cycle arrest after cytokinesis failure and the induction of tetraploidy...
  48. Defining novel pathways that arrest genetically unstable tetraploid cells
    Neil J Ganem; Fiscal Year: 2012
    ..to comprehensively identify proteins that are necessary to activate or maintain G1 cell cycle arrest after cytokinesis failure and the induction of tetraploidy...
  49. Rho GTPases in Terminal Erythroid Maturation
    THEODOSIA ANASTASIOS KALFA; Fiscal Year: 2013
    ..cell imaging in flow we demonstrated evidence that enucleation is a multi-step process resembling asymmetric cytokinesis. It requires establishment of cell polarity through microtubule function, followed by formation of a ..
  50. SEQUENCE DETERMINANTS OF PROTEIN STRUCTURE AND STABILITY
    Robert T Sauer; Fiscal Year: 2013
    ..transport of cargo along microtubules, secretion and vesicle recycling, cell-cycle control, viral budding, cytokinesis, activation of apoptosis and the innate immune response, chromosome translocation, viral DNA packaging, ..
  51. Characterization of nucleotide-dependent structural rearrangements in atlastin
    LAURA JEAN BYRNES; Fiscal Year: 2012
    ..of which participate in diverse cellular functions, including endocytosis, organelle fusion and fission, cytokinesis, and antiviral activity...
  52. Molecular Mechanisms that Regulate Phase Transition in Dimorphic Fungi
    Gregory M Gauthier; Fiscal Year: 2010
    ..I hypothesize that genes involved in environmental sensing, intracellular signaling, cytokinesis, and morphology govern phase transition...
  53. Control of Microtubule and Genome Integrity by the OBSL1-CUL7-CUL9-p53 Pathway
    Yue Xiong; Fiscal Year: 2013
    ..CUL7 and CUL9, two cytoplasmically localized E3 ubiquitin ligases which can bind to p53, control mitosis and cytokinesis by sensing microtubule stress...
  54. Molecular Mechanism of the Contractile-Ring Assembly in Fission Yeast Cytokinesis
    Jian Qiu Wu; Fiscal Year: 2013
    ..by applicant): A significant gap exists in understanding the mechanism of contractile-ring assembly during cytokinesis, which partitions cellular constituents into two new daughter cells and plays a crucial role in cell ..
  55. Cellular Regulation of Dictyostelium Myosin Heavy Chain Kinases
    Paul A Steimle; Fiscal Year: 2012
    ..We propose to examine further how myosin II-dependent cellular activities (specifically, cytokinesis and cellular migration) can be regulated to newly indentified candidate myosin II heavy chain kinases (MHCKs) ..
  56. FUNCTION AND REGULATION OF THE HUMAN ARP2/3 COMPLEX
    Matthew D Welch; Fiscal Year: 2013
    ..cytoskeleton plays an essential role in basic cellular processes including locomotion, membrane transport, and cytokinesis. These processes are important for normal embryonic development, immune system function and tissue repair, and ..
  57. A Mutagenic Study of Yeast Actin Conformational Changes
    Peter A Rubenstein; Fiscal Year: 2010
    ..provided by applicant): Actin is involved in generation of contractile force, cell shape determination, cytokinesis, and determination of cell polarity...
  58. Flowcell for Biological Imaging with LiquidSTEM
    John Damiano; Fiscal Year: 2013
    ..We will study cytokinesis in live yeast cells and, aiming to better understand the molecular mechanism of cytokinesis, we wil study the ..
  59. Signaling Networks in Chemotaxis and Cytokinesis
    Peter N Devreotes; Fiscal Year: 2013
    ..abstract_text> ..
  60. ELMO1, Dock180 and Glioma Invasion
    Shi Yuan Cheng; Fiscal Year: 2012
    ..ELMO1 and its binding partner, dedicator of cytokinesis 1 (Dock180) function as a bipartite guanine nucleotide exchange factor (GEF) that activates Rac1 and promotes ..
  61. Mob1 Localization and Function During Mitosis
    Charles B Shuster; Fiscal Year: 2012
    ..of chromosomal ploidy during cell division requires a precise coordination of chromosome segregation and cytokinesis such that the contractile ring does not assemble before all replicated sister chromatids are correctly attached ..
  62. Cell Cycle control by cyclin-dependent kinases
    DAVID OWEN MORGAN; Fiscal Year: 2013
    ..selected targets in the control of two major processes: chromosome segregation in mitosis and cell division in cytokinesis. The first aim centers on the Cdk substrate Eco1, which promotes the cohesion of sister chromatids as they are ..
  63. Actin Filament Network Remodeling, Disassembly and Turnover
    Bruce L Goode; Fiscal Year: 2013
    ..and thereby clarify the mechanisms driving cell motility, cell morphogenesis, endocytosis, phagocytosis, and cytokinesis. A number of actin binding proteins besides ADF/cofilin have been genetically implicated in promoting actin ..
  64. Automated analysis of cell cycle genes in Trypanosoma brucei
    LAWRENCE RUBEN; Fiscal Year: 2009
    ..coordinated replication and segregation of cellular components, culminating in cleavage furrow formation and cytokinesis. Although the cell cycle is generally conserved, trypanosomes have unique features that prevent identification ..
  65. TOMOGRAPHIC ANALYSIS OF CYTOKINESIS IN ARABIDOPSIS
    L Staehelin; Fiscal Year: 2003
    ..b>Cytokinesis in plants involves the fusion of Golgi-derived vesicles into a cell plate that undergoes a series of complex ..
  66. TOMOGRAPHIC ANALYSIS OF CYTOKINESIS IN ARABIDOPSIS
    L Staehelin; Fiscal Year: 2002
    ..b>Cytokinesis in plants involves the fusion of Golgi-derived vesicles into a cell plate that undergoes a series of complex ..
  67. MOLECULAR ANALYSIS OF MICROTUBULE ORGANIZATION
    Harish Joshi; Fiscal Year: 2003
    ..abnormalities in spindle assembly arrest cells in prometaphase, and entry into anaphase and subsequent cytokinesis is inhibited...
  68. Analysis of the role of patellin in cytokinesis
    T Peterman; Fiscal Year: 2005
    ..of the genetic material during nuclear division, then the partitioning of the cytoplasm and its contents during cytokinesis. Plants partition their cytoplasm by building a new cell wall from the inside-out between the two sets of ..
  69. MOLECULAR ANALYSIS OF CYTOKINESIS IN DICTYOSTELIUM
    Arturo De Lozanne; Fiscal Year: 2006
    Despite recent advances, cytokinesis remains the least understood aspect of the cell cycle...
  70. Microfilament organization in mitosis and transformation
    Fumio Matsumura; Fiscal Year: 2009
    ..Specific aim 1 is to determine the function(s) of MYPT in spindle assembly and cytokinesis. MYPT is phosphorylated by cdc2 kinase, resulting in complex formation with polo-like kinase 1 (PLK1)...
  71. RACE PATHWAY IN THE REGULATION OF CYTOKINESIS
    Denis Larochelle; Fiscal Year: 1999
    An integral aspect of all cancers is the requirement for cell division. Cytokinesis is the final step in cell division, where the cell constricts (usually at the equator) to partition the newly duplicated nuclei, along with half of the ..
  72. CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSIS
    James Spudich; Fiscal Year: 2001
    ..While this process of cytokinesis clearly involves the contractile proteins actin and myosin, which accumulate in the furrow as a contractile ..