enhancer elements genetics


Summary: Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.

Top Publications

  1. ncbi SF-1 (steroidogenic factor-1), C/EBPbeta (CCAAT/enhancer binding protein), and ubiquitous transcription factors NF1 (nuclear factor 1) and Sp1 (selective promoter factor 1) are required for regulation of the mouse aldose reductase-like gene (AKR1B7) expre
    C Aigueperse
    , , , France
    Mol Endocrinol 15:93-111. 2001
  2. ncbi Biological activity of mammalian transcriptional repressors
    G Thiel
    Department of Medical Biochemistry and Molecular Biology, University of Saarland Medical School, Homburg, Germany
    Biol Chem 382:891-902. 2001
  3. ncbi Regulation of laminin beta2 chain gene expression in human cancer cell lines
    M E Durkin
    Institute of Molecular Pathology, University of Copenhagen, Denmark
    Eur J Biochem 268:3797-806. 2001
  4. ncbi Transcription factor-green fluorescent protein chimeric fusion proteins and their use in studies of DNA affinity chromatography
    H W Jarrett
    Department of Biochemistry, University of Tennessee, Memphis 38163, USA
    J Chromatogr A 803:131-9. 1998
  5. ncbi Mechanisms of ligand-induced aryl hydrocarbon receptor-mediated biochemical and toxic responses
    C L Wilson
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station 77843 4466, USA
    Toxicol Pathol 26:657-71. 1998
  6. ncbi Heparin elution of transcription factors from DNA-Sepharose columns
    H Gadgil
    Department of Biochemistry, University of Tennessee, Memphis 38163, USA
    J Chromatogr A 848:131-8. 1999
  7. ncbi Transcriptional regulation of the human apolipoprotein genes
    V I Zannis
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Departments of Medicine and Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118 239, USA
    Front Biosci 6:D456-504. 2001
  8. pmc Function and regulation of gastrin in transgenic mice: a review
    T C Wang
    Department of Medicine, Massachusetts General Hospital, Boston 02114
    Yale J Biol Med 65:705-13; discussion 737-40. 1992
  9. ncbi Comparative studies on discrete and concatemeric DNA-sepharose columns for purification of transcription factors
    H Gadgil
    Department of Biochemistry, University of Tennessee, Memphis 38163, USA
    J Chromatogr A 917:43-53. 2001
  10. ncbi New formulations of demineralized bone matrix as a more effective graft alternative in experimental posterolateral lumbar spine arthrodesis
    G J Martin
    Department of Orthopaedic Surgery and Neurosurgery, Emory University School of Medicine, Atlanta, Georgia, USA
    Spine (Phila Pa 1976) 24:637-45. 1999

Detail Information

Publications276 found, 100 shown here

  1. ncbi SF-1 (steroidogenic factor-1), C/EBPbeta (CCAAT/enhancer binding protein), and ubiquitous transcription factors NF1 (nuclear factor 1) and Sp1 (selective promoter factor 1) are required for regulation of the mouse aldose reductase-like gene (AKR1B7) expre
    C Aigueperse
    , , , France
    Mol Endocrinol 15:93-111. 2001
    ..Taken together, our findings suggest that detoxifying enzymes of the aldose reductase family may constitute new potential targets for regulators of adrenal and gonadal differentiation and function, e.g. SF-1 and DAX-1...
  2. ncbi Biological activity of mammalian transcriptional repressors
    G Thiel
    Department of Medical Biochemistry and Molecular Biology, University of Saarland Medical School, Homburg, Germany
    Biol Chem 382:891-902. 2001
    ..Here we show here that histone deacetylase 2, targeted to the reporter gene via DNA-protein interaction, functions as a transcriptional repressor protein regardless of the location of its binding site within the transcription unit...
  3. ncbi Regulation of laminin beta2 chain gene expression in human cancer cell lines
    M E Durkin
    Institute of Molecular Pathology, University of Copenhagen, Denmark
    Eur J Biochem 268:3797-806. 2001
  4. ncbi Transcription factor-green fluorescent protein chimeric fusion proteins and their use in studies of DNA affinity chromatography
    H W Jarrett
    Department of Biochemistry, University of Tennessee, Memphis 38163, USA
    J Chromatogr A 803:131-9. 1998
    ..This temperature-dependent affinity chromatography provides an important new approach to transcription factor purification...
  5. ncbi Mechanisms of ligand-induced aryl hydrocarbon receptor-mediated biochemical and toxic responses
    C L Wilson
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station 77843 4466, USA
    Toxicol Pathol 26:657-71. 1998
  6. ncbi Heparin elution of transcription factors from DNA-Sepharose columns
    H Gadgil
    Department of Biochemistry, University of Tennessee, Memphis 38163, USA
    J Chromatogr A 848:131-8. 1999
    ..Heparin elution represents an important new tool in the purification of transcription factors and other DNA-binding proteins by DNA affinity chromatography...
  7. ncbi Transcriptional regulation of the human apolipoprotein genes
    V I Zannis
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Departments of Medicine and Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118 239, USA
    Front Biosci 6:D456-504. 2001
    ..Understanding the transcriptional regulatory mechanism of the apolipoprotein genes may allow, in the long run, selective increase of anti-atherogenic lipoproteins and thus reduce the risk of cardiovascular disease...
  8. pmc Function and regulation of gastrin in transgenic mice: a review
    T C Wang
    Department of Medicine, Massachusetts General Hospital, Boston 02114
    Yale J Biol Med 65:705-13; discussion 737-40. 1992
  9. ncbi Comparative studies on discrete and concatemeric DNA-sepharose columns for purification of transcription factors
    H Gadgil
    Department of Biochemistry, University of Tennessee, Memphis 38163, USA
    J Chromatogr A 917:43-53. 2001
    ..Our study shows concatemeric columns do not have any distinct advantage for the three different transcription factors we studied including Sp1, the original justification for the concatemeric approach...
  10. ncbi New formulations of demineralized bone matrix as a more effective graft alternative in experimental posterolateral lumbar spine arthrodesis
    G J Martin
    Department of Orthopaedic Surgery and Neurosurgery, Emory University School of Medicine, Atlanta, Georgia, USA
    Spine (Phila Pa 1976) 24:637-45. 1999
    ..These newer formulations of Grafton appear to have a greater capacity to form bone than the gel form or autogenous bone graft alone in this model...
  11. ncbi Experimental posterolateral lumbar spinal fusion with a demineralized bone matrix gel
    M A Morone
    Department of Orthopaedic Surgery, Emory University School of Medicine, Atlanta, Georgia, USA
    Spine (Phila Pa 1976) 23:159-67. 1998
    ..However, demineralized bone matrix did not increase the frequency of successful fusion when added to the standard amount of autograft...
  12. ncbi High-performance liquid chromatographic assay for diltiazem in small-volume blood specimens and application to pharmacokinetic studies in rats
    P Scully
    Elan Corporation Research Institute, Trinity College, Dublin, Ireland
    J Chromatogr A 729:297-300. 1996
    ..12 +/- 5.43 ng ml-1 h-1 (+/- S.D.)] was higher than that in the control group (11.49 +/- 3.67 ng h-1 ml-1), t-test; p = 0.0483. Hence it was shown that administration of an enhancer could increase the oral bioavailability of diltiazem...
  13. ncbi Cloning and characterization of repressory and stimulatory DNA sequences upstream the Na/I-symporter gene promoter
    T L Schmitt
    Department of Internal Medicine I, University of Ulm, Germany
    Horm Metab Res 32:1-5. 2000
    ..This stimulation is presumably mediated by a putative cAMP responsive element found in the first half of the cloned sequence...
  14. pmc Effect of the putative cognitive enhancer, linopirdine (DuP 996), on quantal parameters of acetylcholine release at the frog neuromuscular junction
    S D Provan
    Department of Pharmacology, James H Quillen College of Medicine, East Tennessee State University, Johnson City 37614 0577
    Br J Pharmacol 111:1103-10. 1994
    ..This appears to involve an effect at the nerve terminal membrane, most likely an increase in Ca2+-conductance, and not an action to block K+-conductance or to release Ca2+ from intraterminal organelles...
  15. ncbi DAX1 gene expression upregulated by steroidogenic factor 1 in an adrenocortical carcinoma cell line
    E Vilain
    Department of Pediatrics, University of California Los Angeles School of Medicine 90095 1752, USA
    Biochem Mol Med 61:1-8. 1997
    ..We conclude that the SF1-RE is an enhancer element within the DAX1 promoter and speculate that SF1 may be a transcription factor that acts, at least in part, through DAX1 for normal adrenal cortical development...
  16. ncbi Transposon tagging and the study of root development in Arabidopsis
    R Tsugeki
    Biotechnology Institute, The Pennsylvania State University, University Park, USA
    Gravit Space Biol Bull 11:79-87. 1998
    ..We find that in addition to eliminating gravitropism, root cap ablation inhibits elongation of roots by lowering root meristematic activities...
  17. ncbi [Neural stem cell, as a source of graft material for transplantation in neuronal disease]
    W Akamatsu
    Division of Neuroanatomy, Department of Neuroscience, Biomedical Research Center, Osaka University Graduate School of Medicine, Suita, Osaka
    No To Hattatsu 33:114-20. 2001
    ..Neural stem cells are a potential, useful source for developing new therapy for CNS disorders...
  18. pmc MDL 26,479: a potential cognition enhancer with benzodiazepine inverse agonist-like properties
    J A Miller
    Marion Merrell Dow Research Institute, Cincinnati, OH 45215
    Br J Pharmacol 107:78-86. 1992
    ..The present study suggests that at least some of the cognition enhancing properties are due to the enhancement of cortical and hippocampal cholinergic function and LTP...
  19. ncbi Immunogenetic properties of genes
    B Wahren
    Microbiology and Tumorbiology Center, Karolinska Institute and Swedish Institute for Infectious Disease Control, Stockholm
    Acta Oncol 38:309-11. 1999
    ..The immunizations produce long-term humoral and cellular responses, qualitatively similar to live attenuated vaccines but without the safety hazards of infectious agents. Recently, the first human DNA vaccinations were reported...
  20. ncbi Multiple elements within the 5' distal enhancer of the mouse heme oxygenase-1 gene mediate induction by heavy metals
    J Alam
    Department of Molecular Genetics, Alton Ochsner Medical Foundation, New Orleans, Louisiana 70121
    J Biol Chem 269:25049-56. 1994
    ..Treatment of Hepa cells with cadmium or heme does not alter the nuclear concentration of AP-1 or C/EBP binding activity...
  21. ncbi AKT induces transcriptional activity of PU.1 through phosphorylation-mediated modifications within its transactivation domain
    P Rieske
    Department of Biochemistry, MCP Hahnemann University School of Medicine, Philadelphia, Pennsylvania 19102, USA
    J Biol Chem 276:8460-8. 2001
    ..1. Taken together, these results provide evidence that PU.1 is induced by AKT signal in a phosphoinositide 3-kinase-dependent manner, leading to inducible or constitutive activation of its target genes...
  22. ncbi Bi-column method for purification of transcription factors
    H Gadgil
    Department of Biochemistry, University of Tennessee, Memphis 38163, USA
    J Chromatogr A 905:133-9. 2001
    ..Highly pure CAAT enhancer binding protein and the B3 transcription factor are also obtained by using variants of this bi-column method...
  23. pmc Tumour metastasis suppressor, nm23-beta, inhibits gelatinase A transcription by interference with transactivator Y-box protein-1 (YB-1)
    Sunfa Cheng
    Department of Medicine, San Francisco Veterans Affair Medical Center, University of California, San Francisco 94121, USA
    Biochem J 366:807-16. 2002
  24. ncbi Transcription factors Oct-1 and C/EBPbeta (CCAAT/enhancer-binding protein-beta) are involved in the glutamate/nitric oxide/cyclic-guanosine 5'-monophosphate-mediated repression of mediated repression of gonadotropin-releasing hormone gene expression
    D D Belsham
    Department of Reproductive Medicine, The Center for Molecular Genetics, University of Califonia, La Jolla 92093, USA
    Mol Endocrinol 14:212-28. 2000
    ..Thus, we conclude that Oct-1 and C/EBPbeta are both downstream transcriptional regulators involved in the repression of GnRH gene expression by the glutamate/NO/ cGMP signal transduction pathway...
  25. ncbi Effects of nicotine on active avoidance learning in rats: sex differences
    O Yilmaz
    Ege University Center for Brain Research, Bornova Izmir, Turkey
    Behav Pharmacol 8:253-60. 1997
    ..6 mg/kg. In conclusion, nicotine pre-treatment affects active avoidance in a sexually dimorphic and dose-dependent manner...
  26. pmc A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes
    L A Gossett
    Department of Biochemistry and Molecular Biology, University of Texas, M D Anderson Cancer Center, Houston 77030
    Mol Cell Biol 9:5022-33. 1989
    ..The ability of MEF-2 to recognize conserved activating elements associated with multiple-specific genes suggests that this factor may participate in the coordinate regulation of genes during myogenesis...
  27. ncbi Biological activity of nasally administered insulin in normal subjects
    M S Nolte
    Metabolic Research Unit, University of California, San Francisco
    Horm Metab Res 22:170-4. 1990
    ..9 +/- 2.3 g for the low dose IN insulin and 34.1 +/- 3.3 g for the high dose IN insulin, the latter value being significantly greater (P less than .01) than the former.(ABSTRACT TRUNCATED AT 250 WORDS)..
  28. ncbi CCAAT enhancer-binding protein alpha suppresses the rat placental glutathione S-transferase gene in normal liver
    Hiromi Ikeda
    Departments of Biochemistry and Ophthalmology, Hokkaido University Graduate School of Medicine, N15, W7, Kita ku, Sapporo 060 8638, Japan
    J Biol Chem 281:6734-41. 2006
    ..These results indicate that C/EBPalpha is a negative regulator of GST-P gene expression in normal liver...
  29. pmc Functional cooperation between CCAAT/enhancer-binding proteins and the vitamin D receptor in regulation of 25-hydroxyvitamin D3 24-hydroxylase
    Puneet Dhawan
    Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA
    Mol Cell Biol 25:472-87. 2005
    ..These findings also indicate a novel role for C/EBPbeta in the cross talk between PTH and 1,25(OH)(2)D(3) that involves the regulation of VDR transcription...
  30. pmc Identification and functional characterization of the human T-cell receptor beta gene transcriptional enhancer: common nuclear proteins interact with the transcriptional regulatory elements of the T-cell receptor alpha and beta genes
    L R Gottschalk
    Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109 0650
    Mol Cell Biol 10:5486-95. 1990
  31. ncbi Notch signaling can inhibit Xath5 function in the neural plate and developing retina
    M L Schneider
    Department of Neurobiology and Anatomy, University of Utah, Salt Lake City 84132, USA
    Mol Cell Neurosci 18:458-72. 2001
    ..Xath5 could upregulate expression of X-Delta-1, ESR1, and ESR3, suggesting that Xath5 participates in a regulatory loop with the Notch pathway...
  32. ncbi [PSI] and [URE3] as yeast prions
    R B Wickner
    Section on Genetics of Simple Eukaryotes, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20892 0830, USA
    Yeast 11:1671-85. 1995
    ..3) Overproduction of Sup35p and Ure2p increases the frequency of cells acquiring [PSI] or [URE3], respectively...
  33. ncbi Human follicle-stimulating hormone receptor (FSH-R) promoter/enhancer activity is inhibited by transcriptional factors, from the upstream stimulating factors family, via E-box and newly identified initiator element (Inr) in FSH-R non-expressing cells
    L T Putowski
    Second Department of Gynecology, University School of Medicine, Lublin, Poland
    Gynecol Endocrinol 19:9-17. 2004
    ..USF family members inhibit FSH-R gene activity by acting via these elements. USF1 and USF2 suppress human FSH-R promoter/enhancer activity by acting on E-box 3. USF1 also decreases activity by interacting with the Inr element...
  34. ncbi Induction of an intronic enhancer of the human ciliary neurotrophic factor receptor (CNTFRalpha) gene by the TR3 orphan receptor
    X m Mu
    Department of Pathology, University of Rochester, NY 14642, USA
    Endocrine 9:27-32. 1998
    ..Our finding may, therefore, suggest CNTFRalpha is a target gene regulated by TR3 and expand the role of TR3 in the nervous system...
  35. ncbi Chick delta1-crystallin enhancer influences mouse alphaA-crystallin promoter activity in transgenic mice
    Lixing W Reneker
    Department of Ophthalmology, University of Missouri, Columbia, 65212, USA
    Invest Ophthalmol Vis Sci 45:4083-90. 2004
    ..This heterogenic enhancer/promoter construct was tested in transgenic mice to see whether it was active in both lens epithelium and fiber cells while retaining lens specificity...
  36. ncbi Liver-specific enhancer of the glucokinase gene
    P B Iynedjian
    Division of Clinical Biochemistry and Diabetes Research, University of Geneva School of Medicine, 1211 Geneva, Switzerland
    J Biol Chem 271:29113-20. 1996
    ..The human gene fragment ligated to the minimal rat liver glucokinase promoter was shown to work as an enhancer in the hepatocyte transfection system...
  37. pmc Effects of DAU 6215, a novel 5-hydroxytryptamine3 (5-HT3) antagonist on electrophysiological properties of the rat hippocampus
    M B Passani
    Dipartimento di Farmacologia Preclinica e Clinica, , Italy
    Br J Pharmacol 112:695-703. 1994
  38. pmc Transactivation of the Moloney murine leukemia virus and T-cell receptor beta-chain enhancers by cbf and ets requires intact binding sites for both proteins
    W Sun
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Virol 69:4941-9. 1995
    ..These findings suggest that ets and cbf proteins cooperate in vivo to regulate transcription from the Mo-MLV and TCR beta enhancers...
  39. pmc VBP and RelA regulate avian leukosis virus long terminal repeat-enhanced transcription in B cells
    S M Curristin
    Department of Microbiology and Immunology and Cancer Center, University of Rochester, School of Medicine and Dentistry, New York 14642, USA
    J Virol 71:5972-81. 1997
    ..The synergistic interaction of VBP and RelA increased CCAAT/enhancer element-mediated transcription, indicating that both factors may be important for viral LTR regulation and also for expression of many cellular genes...
  40. ncbi Adipose expression of the phosphoenolpyruvate carboxykinase promoter requires peroxisome proliferator-activated receptor gamma and 9-cis-retinoic acid receptor binding to an adipocyte-specific enhancer in vivo
    J H Devine
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Biol Chem 274:13604-12. 1999
    ..This work provides the first in vivo evidence that the binding of PPARgamma to its target sequences is absolutely required for adipocyte-specific gene expression...
  41. ncbi [Acquired drives. The cortical mechanism responsible to the emergence and development of social existence]
    Knoll József
    Semmelweis Egyetem, Farmakológiai Bs Farmakoterápiás Intézet, Budapest
    Neuropsychopharmacol Hung 9:151-66. 2007
    ..It seeks to outgrow the myth-directed era of its history and come to its final state, the reason-directed human society...
  42. pmc Regulation of avian leukosis virus long terminal repeat-enhanced transcription by C/EBP-Rel interactions
    W J Bowers
    Department of Microbiology and Immunology, University of Rochester, New York 14642, USA
    J Virol 70:3051-9. 1996
    ..These findings suggest a role for Rel-related factors in the regulation of AIN or RSV LTR-driven transcription via an interaction with Al/EBP...
  43. ncbi NF-kappa B activity is required for the deregulation of c-myc expression by the immunoglobulin heavy chain enhancer
    K Kanda
    Center for Molecular Biology in Medicine, Veterans Affairs Palo Alto Health Care System and the Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
    J Biol Chem 275:32338-46. 2000
  44. pmc a1/EBP: a leucine zipper protein that binds CCAAT/enhancer elements in the avian leukosis virus long terminal repeat enhancer
    W J Bowers
    Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642
    J Virol 66:6578-86. 1992
    ..2-kb cDNA encodes a 209-amino-acid open reading frame containing carboxy-terminal basic and leucine zipper motifs, indicating that a1/EBP encodes a novel member of the leucine zipper family of transcription factors...
  45. ncbi Pharmacokinetics of NS-105, a novel cognition enhancer. 1st communication: absorption, metabolism and excretion in rats, dogs and monkeys after single administration of 14C-NS-105
    H Mukai
    Research Laboratories, Nippon Shinyaku Co, Ltd, Kyoto, Japan
    Arzneimittelforschung 49:881-90. 1999
    ..4% of the dose, and lymphatic absorption of radioactivity was only 0.3% of the dose. The percentage of 14C-NS-105 bound to serum proteins was less than 3.3% in all the animal species tested, including humans...
  46. pmc Transcription factor Oct-2A contains functionally redundant activating domains and works selectively from a promoter but not from a remote enhancer position in non-lymphoid (HeLa) cells
    M M Müller-Immerglück
    Institut fur Molekularbiologie II, Universitat Zurich, Switzerland
    EMBO J 9:1625-34. 1990
    ..From these experiments we consider it likely that the natural octamer factor variants can selectively activate the different naturally occurring octamer-containing promoters...
  47. pmc Stimulation of Tat-associated kinase-independent transcriptional elongation from the human immunodeficiency virus type-1 long terminal repeat by a cellular enhancer
    M J West
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    EMBO J 18:1378-86. 1999
    ..Thus, the activation of polymerase processivity by the IgHE involves a unique mechanism which is independent of TAK...
  48. pmc Distinct functions of CCAAT enhancer-binding protein isoforms in the regulation of manganese superoxide dismutase during interleukin-1beta stimulation
    Xiaolei Qiu
    McKnight Brian Institute, University of Florida, Gainesville, Florida 32610, USA
    J Biol Chem 283:25774-85. 2008
    ..Our data demonstrating the physiological relevance of the N-terminal peptide also provide a rationale for revisiting the role of LAP* in the regulation of other genes and in pathways such as lipogenesis and development...
  49. ncbi The archetype enhancer of simian virus 40 DNA is duplicated during virus growth in human cells and rhesus monkey kidney cells but not in green monkey kidney cells
    Frank J O'Neill
    Department of Veterans Affairs, Salt Lake City Health Care System, 500 Foothill Boulevard, Salt Lake City, UT 84148, USA
    Virology 310:173-82. 2003
    ..These findings may be of relevance to studies attempting to detect SV40 DNA in human tumors or other clinical specimens...
  50. pmc An RNA splicing enhancer-like sequence is a component of a splicing inhibitor element from Rous sarcoma virus
    L M McNally
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Mol Cell Biol 18:3103-11. 1998
    ..Our results are consistent with a role for the purine region in facilitated snRNP binding to the NRS via SF2/ASF...
  51. ncbi An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells
    Jesper T Troelsen
    Department of Medical Biochemistry and Genetics, Biochemical Laboratory C, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK 2200 N Copenhagen, Denmark
    Gene 305:101-11. 2003
    ..The LPH enhancer contains at least three cis-elements (at -894 to -880, -880 to -875 and -833 to -814) with functional importance for the LPH enhancer activity...
  52. pmc Activation of the human epsilon- and beta-globin promoters by SV40 T antigen
    S X Cao
    Laboratory of Chemical Biology, National Institute of Diabetes, and Digestive and Kidney Diseases, Bethesda, MD 20892
    Biochem J 258:769-76. 1989
  53. ncbi Structure-activity relationship for chemical skin permeation enhancers: probing the chemical microenvironment of the site of action
    Kevin S Warner
    Department of Pharmaceutics and Pharmaceutical Chemistry, 213 Skaggs Hall, University of Utah, 30 S 2000 E, Salt Lake City, Utah 84112, USA
    J Pharm Sci 92:1305-22. 2003
  54. ncbi Enhancer blocking by chicken beta-globin 5'-HS4: role of enhancer strength and insulator nucleosome depletion
    Hui Zhao
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:30573-80. 2006
    ..These findings provide evidence that a domain of active chromatin is formed by spreading from an enhancer to a target gene and can be blocked by a nucleosome-free gap in an insulator...
  55. pmc Transcriptional targeting of lentiviral vectors by long terminal repeat enhancer replacement
    Francesco Lotti
    TIGET, Istituto Scientifico H San Raffaele, 20132 Milan, Italy
    J Virol 76:3996-4007. 2002
  56. ncbi Isolation and characterization of a cDNA clone for the CCAAT transcription factor EFIA reveals a novel structural motif
    J Ozer
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
    J Biol Chem 265:22143-52. 1990
    ..The functional significance of this novel structural motif, which is also conserved in dbpA, FRG Y1, and FRG Y2, will be discussed...
  57. pmc Rat cytomegalovirus major immediate-early enhancer switching results in altered growth characteristics
    G R Sandford
    Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Virol 75:5076-83. 2001
    ..Four months after infection, recombinant virus with the foreign MIE enhancer was reactivated from spleen explants...
  58. pmc Adipocyte-specific expression of murine resistin is mediated by synergism between peroxisome proliferator-activated receptor gamma and CCAAT/enhancer-binding proteins
    Takuya Tomaru
    Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, The Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6149, USA
    J Biol Chem 284:6116-25. 2009
  59. ncbi Cellular specificity of HIV-1 replication can be controlled by LTR sequences
    Edward Reed-Inderbitzin
    Division of Basic Biomedical Sciences, University of South Dakota, Vermillion, SD 57069, USA
    Virology 314:680-95. 2003
    ..This investigation demonstrates that cellular specificity can be imparted onto HIV-1 replication at the level of viral transcription and not entry...
  60. pmc The coupling between enhancer activity and hypomethylation of kappa immunoglobulin genes is developmentally regulated
    D E Kelley
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
    Mol Cell Biol 8:930-7. 1988
    ..The change in methylation status, together with associated changes in chromatin structure, may suffice to eliminate or lessen the importance of the enhancer for the maintenance of the transcriptionally active state...
  61. ncbi Transcriptional mechanisms of chondrocyte differentiation
    B de Crombrugghe
    Department of Molecular Genetics, The University of Texas, M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Matrix Biol 19:389-94. 2000
    ..These results favor the hypothesis that in achondroplasia, a disease caused by activating mutations in FGF receptor 3, there might also be an abnormally high Sox9 expression...
  62. ncbi A conserved cluster of homeodomain binding sites in the mouse Hoxa-4 intron functions in Drosophila embryos as an enhancer that is directly regulated by Ultrabithorax
    T E Haerry
    Biozentrum, University of Basel, Switzerland
    Dev Biol 186:1-15. 1997
    ..The evolutionary and functional conservation of the HB1 elements indicates that not only the homeodomains but also some of their in vivo binding sites are conserved between vertebrates and invertebrates...
  63. pmc A new regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1 and cloning of cDNAs encoding its binding proteins
    A Tanimura
    Department of Cellular and Molecular Biology, University of Tokyo, Japan
    J Virol 67:5375-82. 1993
    ..These results suggest that binding of THP to TRE-2S may be involved in cooperation with one copy of the 21-bp enhancer in responding to Tax trans activation...
  64. ncbi Role of the LTR region between the enhancer and promoter in mink cell focus-forming murine leukemia virus pathogenesis
    F K Yoshimura
    Department of Immunology and Microbiology, Wayne State University, 540 E Canfield Ave, Detroit, MI 48201, USA
    Virology 283:121-31. 2001
    ..The lack of DEN virus expression by tumor cells led us to propose that DEN sequences may play a role at an early step in tumorigenesis...
  65. ncbi SOX7 and SOX17 regulate the parietal endoderm-specific enhancer activity of mouse laminin alpha1 gene
    Tomoaki Niimi
    Sekiguchi Biomatrix Signaling Project, ERATO, Japan Science and Technology Agency, Aichi Medical University, Karimata, Yazako, Nagakute, Aichi 480 1195, Japan
    J Biol Chem 279:38055-61. 2004
  66. pmc The human granulocyte-macrophage colony-stimulating factor gene is autonomously regulated in vivo by an inducible tissue-specific enhancer
    P N Cockerill
    Division of Human Immunology, Hanson Centre for Cancer Research, Institute for Medical and Veterinary Science, Frome Road, Adelaide 5000, Australia
    Proc Natl Acad Sci U S A 96:15097-102. 1999
  67. ncbi Regulation of the twist target gene tinman by modular cis-regulatory elements during early mesoderm development
    Z Yin
    Brookdale Center for Developmental and Molecular Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Development 124:4971-82. 1997
  68. ncbi Repression of enhancer activity by the adenovirus E1A tumor protein(s) is mediated through a novel HeLa cell nuclear factor
    A E Reifel-Miller
    Department of Molecular Genetics, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285
    J Biol Chem 266:13873-82. 1991
    ..Overall, our data demonstrate that an enhancer can be repressed by the trans-activation of a negative regulatory factor...
  69. ncbi Identification of a novel dexamethasone responsive enhancer in the human CYP3A5 gene and its activation in human and rat liver cells
    J D Schuetz
    Department of Pharmaceutical Sciences, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    Mol Pharmacol 49:63-72. 1996
    ..This is the first study to demonstrate that a member of the human CYP3A gene family contains an enhancer that binds the GCR and that this binding is critical to transcriptional activation by dexamethasone...
  70. ncbi Ig/EBP-1: a ubiquitously expressed immunoglobulin enhancer binding protein that is similar to C/EBP and heterodimerizes with C/EBP
    C Roman
    Department of Biological Chemistry, University of California, Los Angeles
    Genes Dev 4:1404-15. 1990
    ..Finally, elevated expression of Ig/EBP-1 in early B cells may explain in part the enhancer-independent activity of VH promoters early in B-cell development...
  71. ncbi [Screening and application of enhancer-like sequences from vaccinia virus]
    Feng Han
    Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
    Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 21:301-3. 2007
    ..To screen enhancer-like sequences from vaccinia virus genome, to construct an expression vector harboring prokaryotic enhancer-like sequence and study the effect of interferon gene expression...
  72. ncbi Stimulation of lymphoproliferation by sucrose esters
    H M Therien
    Département Chimie Biologie, Universite du Quebec a Trois Rivieres, Canada
    Immunopharmacol Immunotoxicol 11:603-9. 1989
    ..These properties of sucrose esters are discussed in the specific context of antifungal therapy...
  73. doi MiR-101 inhibits melanoma cell invasion and proliferation by targeting MITF and EZH2
    Chonglin Luo
    Department of Translational Immunology, German Cancer Research Center DKFZ, Heidelberg, Germany Electronic address
    Cancer Lett 341:240-7. 2013
    ..Functional assays showed that miR-101 suppressed invasion and proliferation - an outcome that could be phenocopied by siRNA knockdown of MITF and EZH2. Our data suggest that miR-101 might have a beneficial role in melanoma. ..
  74. doi Guaifenesin derivatives promote neurite outgrowth and protect diabetic mice from neuropathy
    Mallinath B Hadimani
    Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, Greensboro, North Carolina 27412, USA
    J Med Chem 56:5071-8. 2013
    ..Findings revealed here could lead to the development of novel drugs for diabetic neuropathy. ..
  75. ncbi A global control region defines a chromosomal regulatory landscape containing the HoxD cluster
    Francois Spitz
    Department of Zoology and Animal Biology, NCCR Frontiers in Genetics, University of Geneva, Sciences III, Quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland
    Cell 113:405-17. 2003
    ..These domains were interrupted in the Ulnaless mutation, a balanced inversion that modified the topography of the locus. We discuss the heuristic value of these results in term of locus specific versus gene-specific regulation...
  76. pmc Lineage-specific purification of neural stem/progenitor cells from differentiated mouse induced pluripotent stem cells
    Masato Maruyama
    Department of Anatomy and Brain Science, Kansai Medical University, Hirakata, Osaka, Japan
    Stem Cells Transl Med 2:420-33. 2013
    ..Our results suggest that a lineage-specific drug selection strategy is useful for purification of NSCs from differentiated iPS cells and that this strategy can be applied for the purification of other cell types...
  77. doi Anti-adipogenic diarylheptanoids from Alnus hirsuta f. sibirica on 3T3-L1 cells
    Mina Lee
    College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Daehak dong, San 56 1, Sillim dong, Gwanak Gu, Seoul 151 742, Republic of Korea
    Bioorg Med Chem Lett 23:2069-73. 2013
    ..It was demonstrated that compound 7 has anti-adipogenic activity mediated by the regulation of PPARγ dependent pathways...
  78. doi Exendin-4 attenuates high glucose-induced cardiomyocyte apoptosis via inhibition of endoplasmic reticulum stress and activation of SERCA2a
    Craig W Younce
    Metabolic Signaling and Disease Program, Diabetes and Obesity Research Center, Sanford Burnham Medical Research Institute at Lake Nona, 6400 Sanger Rd, Orlando, FL 32837, USA
    Am J Physiol Cell Physiol 304:C508-18. 2013
    ..These findings identify a novel mechanism whereby Glp1-based therapies could be used as treatments for diabetic cardiomyopathy...
  79. doi SENP2 regulates MEF2A de-SUMOylation in an activity dependent manner
    Han Lu
    Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine SJTU SM, 197 Ruijin Er Road, Shanghai, 200025, People s Republic of China
    Mol Biol Rep 40:2485-90. 2013
    ..Our data clearly show that SENP2 plays an important role in determining the dynamics and functional outcome of MEF2A SUMOylation and transcriptional activation...
  80. pmc Phosphorylation of serine 248 of C/EBPα is dispensable for myelopoiesis but its disruption leads to a low penetrant myeloid disorder with long latency
    Marie S Hasemann
    The Finsen Laboratory, Rigshospitalet, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 7:e38841. 2012
    ..In particular, the phosphorylation of serine 248 of the transactivation domain has been shown to be of crucial importance for granulocytic differentiation of 32Dcl3 cells in vitro...
  81. pmc Autonomic neurocristopathy-associated mutations in PHOX2B dysregulate Sox10 expression
    Mayumi Nagashimada
    Laboratory for Neuronal Differentiation and Regeneration, RIKEN Center for Developmental Biology, Kobe, Japan
    J Clin Invest 122:3145-58. 2012
    ..Our results also demonstrate that Sox10 regulation by PHOX2B is pivotal for the development and pathogenesis of the autonomic ganglia...
  82. doi Paradoxical effects of antioxidants on cancer
    Andrew R Mendelsohn
    Panorama Research Institute and Regenerative Sciences Institute, Sunnyvale, California
    Rejuvenation Res 17:306-11. 2014
    ..Personal genomic analysis may provide the means to identify context to avoid these paradoxes and obtain a successful outcome. However, cancer prevention may be more difficult than previously thought...
  83. doi HPMC as a potential enhancer of nimodipine biopharmaceutical properties via ball-milled solid dispersions
    Manoela Klüppel Riekes
    Programa de Pós graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis 88040 970, Brazil Electronic address
    Carbohydr Polym 99:474-82. 2014
    ..The increase in the stability observed when the HPMC concentration was increased from 70 to 90% in the solid dispersions was attributed to the dilution mechanism...
  84. doi Inhibition of PP2A activity confers a TRAIL-sensitive phenotype during malignant transformation
    Hongmei Yang
    Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan 430022, Hubei Province, China
    Mol Cancer Res 12:217-27. 2014
  85. pmc INO80 governs superenhancer-mediated oncogenic transcription and tumor growth in melanoma
    Bingying Zhou
    State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Genes Dev 30:1440-53. 2016
    ..Together, our results reveal an essential role of INO80-dependent chromatin remodeling in SE function and suggest a novel strategy for disrupting SEs in cancer treatment...
  86. doi Induction of Gnrh mRNA expression by the ω-3 polyunsaturated fatty acid docosahexaenoic acid and the saturated fatty acid palmitate in a GnRH-synthesizing neuronal cell model, mHypoA-GnRH/GFP
    Dean Q Tran
    Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada
    Mol Cell Endocrinol 426:125-35. 2016
    ..This will advance our understanding of the mechanisms underlying FFA sensing in the brain and provides insight into the links between nutrition and reproductive function. ..
  87. ncbi The silencer activity of the novel human serotonin transporter linked polymorphic regions
    Kazuo Sakai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, 1 1 Rokko dai, Nada, Kobe 657 8501, Japan
    Neurosci Lett 327:13-6. 2002
    ..The categorized genotypic or allelic frequencies was not significantly different between the mood disorder and the control. No significant difference was detected either when analyzed by silencer activities of each allelic variant...
  88. ncbi Gastrin stimulates cyclooxygenase-2 expression in intestinal epithelial cells through multiple signaling pathways. Evidence for involvement of ERK5 kinase and transactivation of the epidermal growth factor receptor
    Yan Shi Guo
    Departments of Surgery and Ophthalmology, University of Texas Medical Branch, Galveston 77555, USA
    J Biol Chem 277:48755-63. 2002
    ..Thus, our findings identify the signaling pathways coupling the CCK-B receptor with up-regulation of COX-2 expression. This effect may contribute to this hormone-dependent gastrointestinal carcinogenesis, especially in the colon...
  89. ncbi CCAAT/enhancer binding proteins are expressed in the gerbil hippocampus after transient forebrain ischemia
    Youji Soga
    Department of Neurosurgery, Brain Research Institute, Niigata University, 1 757 Asahimachi Niigata, Japan
    Neurosci Lett 337:106-10. 2003
  90. doi Peroxiredoxin II promotes hepatic tumorigenesis through cooperation with Ras/Forkhead box M1 signaling pathway
    Y H Park
    Aging Intervention Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
    Oncogene . 2015
    ..Oncogene advance online publication, 26 October 2015; doi:10.1038/onc.2015.411...
  91. doi Dynamic changes in global and gene-specific DNA methylation during hibernation in adult thirteen-lined ground squirrels, Ictidomys tridecemlineatus
    Sebastian Alvarado
    Department of Pharmacology, McGill University, 3655 Sir William Osler Suite 1309, Montreal, QC, Canada H3G 1Y6 Sackler program in Epigenetics and Psychobiology at McGill University
    J Exp Biol 218:1787-95. 2015
    ..Taken together, these data show that genomic DNA methylation is dynamic across torpor-arousal bouts during winter hibernation, consistent with a role for this regulatory mechanism in contributing to the hibernation phenotype. ..
  92. pmc Fine-tuning of NFκB by glycogen synthase kinase 3β directs the fate of glomerular podocytes upon injury
    Hui Bao
    1 Department of Nephrology, Shanghai Tenth People s Hospital, Tongji University School of Medicine, Shanghai, China 2 Division of Kidney Disease and Hypertension, Department of Medicine, Rhode Island Hospital, Brown University School of Medicine, Providence, Rhode Island, USA
    Kidney Int 87:1176-90. 2015
    ..Thus, the GSK3β-dictated fine-tuning of NFκB may serve as a novel therapeutic target for podocytopathy. ..
  93. doi Postnatal expression patterns and polymorphism analysis of the bovine myocyte enhancer factor 2C (Mef2C) gene
    E Juszczuk-Kubiak
    Department of Molecular Cytogenetics, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec, Poland Electronic address
    Meat Sci 98:753-8. 2014
    ..Furthermore, we found that the Mef2C promoter variant (GU211004:g.-1606C>T) does not affect the level of mRNA in the LD and ST muscles of 12-month-old HO bulls...
  94. doi Trichostatin A effectively induces apoptosis in chronic lymphocytic leukemia cells via inhibition of Wnt signaling and histone deacetylation
    Lukas Peiffer
    Department I of Internal Medicine, University at Cologne, Kerpener Strasse 62, 50937, Cologne, Germany
    J Cancer Res Clin Oncol 140:1283-93. 2014
    ..Functionally, this results in increased resistance to apoptosis. We therefore assumed that targeting members of the pathway could reveal new therapeutic options for the treatment of CLL...
  95. pmc Regulation of the human GLUT4 gene promoter: interaction between a transcriptional activator and myocyte enhancer factor 2A
    John B Knight
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, P O Box 26901, Room 853 BMSB, Oklahoma City, OK 73190, USA
    Proc Natl Acad Sci U S A 100:14725-30. 2003
    ..Finally, GEF and MEF2A coimmunoprecipitated in vivo, strongly supporting a mechanism of GLUT4 transcription activation that depends on this protein-protein interaction...
  96. doi New polymorphisms in human MEF2C gene as potential modifier of hypertrophic cardiomyopathy
    Cristina Alonso-Montes
    Bone and Mineral Research Unit, Hospital Universitario Central de Asturias, Oviedo, Spain
    Mol Biol Rep 39:8777-85. 2012
  97. ncbi Synthetic ceramide analogues as skin permeation enhancers: structure-activity relationships
    Katerina Vavrova
    Department of Inorganic and Organic Chemistry, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05, Hradec Kralove, Czech Republic
    Bioorg Med Chem 11:5381-90. 2003
    ..The described relationships could bring more rational approaches in designing new potent enhancers for transdermal formulations...
  98. ncbi Linking C5 deficiency to an exonic splicing enhancer mutation
    Nicole Pfarr
    Children s Hospital of Johannes Gutenberg University of Mainz, Mainz, Germany
    J Immunol 174:4172-7. 2005
    ..This apparently inconsequential sequence alteration represents a noncanonical splicing mutation altering an ESE. Our finding sheds a new light on the role of putative silent/conservative mutations in disease-associated genes...
  99. doi The IkappaBalpha gene is a peroxisome proliferator-activated receptor cardiac target gene
    Norman E Buroker
    Department of Cardiology, Seattle Children s Hospital, WA 98195, USA
    FEBS J 276:3247-55. 2009
    ..In conclusion, our study revealed that PPAR regulates the mouse cardiac IkappaBalpha gene in both control and transgenic mouse heart. The implications of this finding are discussed in relation to possible changes in cardiac function...
  100. doi OH-2, a hyperdiploid myeloma cell line without an IGH translocation, has a complex translocation juxtaposing MYC near MAFB and the IGK locus
    Thea Kristin Våtsveen
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
    Leuk Res 33:1670-7. 2009
    ..OH-2 provides a unique example of an HMCL and the corresponding primary tumor that are shown to share the same HRD genotype...
  101. ncbi Characterization of a novel WHSC1-associated SET domain protein with H3K4 and H3K27 methyltransferase activity
    Sung Mi Kim
    Department of Life Science, College of Natural Sciences, Chung Ang University, Seoul 156 756, South Korea
    Biochem Biophys Res Commun 345:318-23. 2006
    ..Overexpression of WHISTLE repressed transcription of the SV40 promoter. Our results suggest that WHISTLE is a novel SET domain containing a protein with specific H3K4 and H3K27 HMTase activity...

Research Grants71

  1. H1R Signaling and Immune Deviation in EAE
    Cory Teuscher; Fiscal Year: 2013
  2. Air Pollution on Adiposity and Vascular Dysfunction: White or Brown Matters?
    Qinghua Sun; Fiscal Year: 2013
  3. Regulation of ER stress-induced cell death
    Randal J Kaufman; Fiscal Year: 2013
    ..The proposed studies on protein synthesis will provide vital information that should encourage the development of novel therapeutics to treat diabetes. ..
  4. Med1 in Liver Metabolism, Regeneration and Cancer
    Janardan K Reddy; Fiscal Year: 2013
    Lisa M Salati; Fiscal Year: 2012
    ..This project will provide important new data on the ways in which dietary fat can regulate intracellular metabolism. ..
    DHAN V KALVAKOLANU; Fiscal Year: 2013
    ..Studies proposed in this application will investigate the regulation of an anti-metastatic gene, DAPK1, which is critical for tumor suppression and autophagic responses. ..
  7. Exome re-sequencing candidate loci for familial essential tremor
    Barry E Kosofsky; Fiscal Year: 2012
    ..Studying the untoward effects of mutant ET genes will increase our knowledge of ET and other movement disorders such as Parkinson disease. ..
  8. Inhibition of bone formation by Prx1
    Mark S Nanes; Fiscal Year: 2013
    ..Understanding this mechanism will allow us to find new medications that stimulate new bone formation and regeneration of tissue in veterans who suffer from arthritis, aging, and menopause associated fractures. ..
  9. Regulation of Astrocyte TIMP-1 in HIV-Associated Dementia
    JEREL ADAM FIELDS; Fiscal Year: 2011
    ..Our studies will provide a better understanding of the specific mechanistic contributions of activated astrocytes to HIV-1-neuropathogensis and neuroinflammation. ..
  10. Hepatic Steatosis and ER Stress-Inducible Transcription Factor CREBH
    Kezhong Zhang; Fiscal Year: 2013
    ..Understanding the stress-induced molecular mechanisms in hepatic lipid accumulation and its associated pathogenesis is a key prerequisite for the development of new diagnostics and therapeutics targeting NAFLD. ..
  11. Retroviral Vector-mediated Liver Gene Therapy for MPS I
    Katherine P Ponder; Fiscal Year: 2013
    ..This study will also test to see if there are any adverse effects of gene therapy. This may result in a treatment for patients with this severe genetic disease. ..
  12. Genomic Enhancers at 8q24 and Prostate Cancer
    Gerhard A Coetzee; Fiscal Year: 2013
  13. Regulation of insulator function and globin gene expression by USF and associated
    Suming Huang; Fiscal Year: 2013
    ..The studies will provide a novel insight into USF function in the regulation of developmental stage-specific globin gene expression and will lead to novel strategies for the molecular therapy of anemia. ..
  14. Repression of PPARgamma-2 by Glucocorticoid-Induced Leucine Zipper
    Xing Ming Shi; Fiscal Year: 2012
    ..Thus, understanding how PPAR(2 can be negatively regulated, a focus of this study, may lead to new therapies for treating osteoporosis and obesity. ..
  15. Human Heme Oxygenase-1 Gene Regulation in Renal Injury
    Anupam Agarwal; Fiscal Year: 2012
    ..The results of these studies will also be applicable to other disease settings such as atherosclerosis, sepsis and transplant rejection wherein heme oxygenase-1 plays an important protective role. ..
    Michael R Stallcup; Fiscal Year: 2013
    ..The new basic knowledge obtained in this project will identify new potential targets for therapeutic intervention in these diseases. ..
  17. Genomic approaches to inner ear hair cell regeneration
    Michael Lovett; Fiscal Year: 2010
    ..The long-term goal of our research is to develop methods to regenerate and maintain inner ear hair cells by studying the regenerative process in birds and comparing it to mammalian hair cell growth. ..
  18. Smooth muscle cell lipid metabolism and serum amyloid A
    Barbara M Schreiber; Fiscal Year: 2010
    ..Cardiovascular disease remains a major public health concern. Elucidation of mechanisms that promote disease vs. protect against it will lead to future strategies for drug development. ..
    CURT DANIEL SIGMUND; Fiscal Year: 2013
    ..These studies will link transcriptional signals regulating the renin gene with physiological endpoints controlling blood pressure and metabolism which may go awry in hypertension. I..
  20. Dysfunctional PGC-1alpha expression in skeletal muscle during diabetes
    S Russ Price; Fiscal Year: 2013
    ..Use of Cn inhibitors could have a negative impact on both types of patients. Lastly, our results could be broadly applicable to Veterans suffering from many types of debilitating diseases associated with atrophy. ..
    Eric E Turner; Fiscal Year: 2010
  22. Transcriptional regulation of enteroendocrine cell differentiation by NeuroD
    Andrew B Leiter; Fiscal Year: 2013
    ..Genome-wide chromatin occupancy studies (ChIPseq) will identify a subset of differentially expressed genes as potential direct targets by NeuroD. ..
  23. A. L. lactis-based vaccine for children with broad spectrum for enteric pathogens
    Wendy L Picking; Fiscal Year: 2013
    ..Advantages of this vaccine technology include: an outstanding safety profile, broad protection against multiple pathogens, ease of delivery (mucosal vaccination) and simple and inexpensive production. ..
  24. Epigenome Mapping in Cortical Interneurons
    Schahram Akbarian; Fiscal Year: 2013
  25. Functional Assessment of the Locus for Atrial Fibrillation on Chromosome 4q25
    PATRICK THOMAS ELLINOR; Fiscal Year: 2010
    ..We propose to screen this region of susceptibility in zebrafish, cell lines, and mice for functional elements that may lead to atrial fibrillation. ..
  26. Long-Range Genetic and Epigenetic Control of Igh Gene Assembly
    Eugene M Oltz; Fiscal Year: 2010
    ..We propose studies to address how precursor B cells control long-range changes in the spatial organization of the immunoglobulin heavy chain locus to ensure its proper assembly and pursuant generation of an adaptive immune system. ..
  27. Identification of the transcriptional regulators of chondrocyte hypertrophy
    ANDREW BRUCE LASSAR; Fiscal Year: 2013
  28. Integration of Signaling Pathways Regulating Chondrocyte Differentiation
    Sherrill L Adams; Fiscal Year: 2010
    ..The proposed experiments will provide significant insights into the roles these growth factors and hormones play in failure of normal skeletal growth, resulting in short stature. ..
  29. Function and regulation of Lhx genes in craniofacial development
    Juhee Jeong; Fiscal Year: 2010
  30. Detoxification of Electrophilic Chemical Threat Agents by Nucleophilic Scavengers
    Michael C MacLeod; Fiscal Year: 2010
    ..In addition, we will obtain data that indicates whether or not individuals with variants of a particular class of genes, the GSTs, are more susceptible to the effects of SNM toxicants. ..
    Mario Chojkier; Fiscal Year: 2012
    ..These studies are relevant to the hepatocyte proliferation and survival characteristic of many physiological and pathological conditions, including hepatocellular injury, liver regeneration and hepatocellular carcinoma. ..
    John F Engelhardt; Fiscal Year: 2013
    ..Furthermore, a greater knowledge of stem cell biology in the airway will greatly benefit the development genetic-based therapies for inhibited disease such as CF. ..
  33. Gene Therapy of Mucopolysaccharidosis VII
    Mark E Haskins; Fiscal Year: 2012
    ..The dogs and children with this and similar lysosomal storage diseases have abnormal corneas, heart valves, skeleton, and brain and the approaches we are testing are to determine a long-term safe and effective therapy. ..
  34. c-Myc transcription in intestinal growth, differentiation, and carcinogenesis
    Gregory S Yochum; Fiscal Year: 2012
    ..Our studies address the fundamental mechanisms underlying b-catenin regulation of normal gastrointestinal development and homeostasis as well as the pathogenesis of intestinal malignancy. ..
  35. SH2-B regulation of body weight and energy homeostasis
    Liangyou Rui; Fiscal Year: 2010
    ..3. Determine whether SH2-B differentially regulates leptin sensitivity in the LEPRb-neurons governing energy intake versus energy expenditure. ..
  36. Molecular mechanisms of C/EBP alpha mediated growth arrest
    Nikolai A Timchenko; Fiscal Year: 2013
    ..The elucidation of these mechanisms will provide basement for the generation of therapeutic approaches to prevent tumors. ..
  37. Modulating calcineurin signaling pathways in muscle
    Beverly A Rothermel; Fiscal Year: 2012
    ..abstract_text> ..
  38. Roles of Epstein-Barr virus nuclear antigens 2 and LP in B cell proliferation
    Elliott D Kieff; Fiscal Year: 2013
  39. Regulation of glucose homeostasis by C/EBPB
    Jacob E Friedman; Fiscal Year: 2010
  40. Molecular mechanisms of neural plasticity in drug addiction
    LAURA NICOLE SMITH; Fiscal Year: 2010
    ..The goal of this project is to understand how these changes occur, which may lead to targets for potential treatment or prevention of addiction. ..
    Pamela L Mellon; Fiscal Year: 2013
    ..This multifaceted approach should yield a comprehensive understanding of the program of GnRH neuronal migration and cell fate in vivo and in vitro and provide insight into the genetics of hypogonadotropic hypogonadism. ..
  42. Basis for Lymphomagenesis in Akt2 Transgenic Mice
    Joseph R Testa; Fiscal Year: 2013
    ..abstract_text> ..
  43. Viral Gene Expression to Therapeutic Levels in Models of Heart Failure
    Joseph E Rabinowitz; Fiscal Year: 2012
    ..An end point for this application is to determine the therapeutic dose of a therapeutic gene delivered by an experimental gene transfer vector. ..
  44. Transcriptional and epigenomic control of adipose tissue development and function
    David J Steger; Fiscal Year: 2013
  45. Lymphatic Regulation of Skin Electrolyte Metabolism and Blood Pressure
    JENS MARC TITZE; Fiscal Year: 2013
    ..Finally, we will test whether macrophage-derived VEGF-C exerts its blood pressure-lowering effect via receptor binding to blood vessels, or by binding to cutaneous lymph vessels (Aim 3). ..
  46. Proopiomelanocortin gene expression and obesity
    MALCOLM JAMES LOW; Fiscal Year: 2013
    Charles P Emerson; Fiscal Year: 2013
  48. Accessibility Control of Antigen Receptor Gene Assembly
    Eugene M Oltz; Fiscal Year: 2013
    ..We propose studies to address how precursor T lymphocytes activate the T cell receptor beta locus at the earliest stages of thymocyte development to ensure its proper assembly by V(D)J recombination. ..
  49. Characterization of Enhancers in the Epidermal Differentiation Complex
    CRISTINA DE GUZMAN STRONG; Fiscal Year: 2012
    ..abstract_text> ..
    Pamela L Mellon; Fiscal Year: 2011
    ..abstract_text> ..
  51. RANKL and Inflammation-associated Bone Loss
    CHARLES A O'BRIEN; Fiscal Year: 2012
    ..In summary, the studies proposed in this Merit application are relevant to several major causes of localized as well as systemic bone loss and are thus important to the veterans patient health care mission. ..
  52. Molecular genetic control of the immature female mosquito vector
    Helen Benes; Fiscal Year: 2012
  53. Epigenetic Regulation of Epstein-Barr Virus Latency Programs
    Paul M Lieberman; Fiscal Year: 2013
    ..The proposed studies should further our understanding of EBV latent cycle gene regulation and host-virus interactions. ..
  54. Liver Inflammation and Injury: Molecular Mechanisms
    Mario Chojkier; Fiscal Year: 2013
    ..Development of effective treatments for liver inflammation and injury may also facilitate future treatments for lung and kidney inflammation and injury. ..
  55. Regulatory architecture of the Androgen Receptor locus in development & evolution
    Philip L Reno; Fiscal Year: 2010
    ..Identifying the mechanisms by which AR expression is regulated may aid in the long-term diagnosis and treatment of these and other human diseases. ..
  56. Regulation of Antioxidant Genes and Oxidative Stress
    Yoshiaki Tsuji; Fiscal Year: 2013
    ..This proposal will also investigate the common regulatory mechanism of ferritin and other antioxidant genes that are involved in cellular defense mechanisms against oxidative stress. ..
  57. Characterization of Chd7 in central nervous system neural stem cells
    JOSEPH ANTHONY MICUCCI; Fiscal Year: 2013
    ..Aim 3: Elucidate CHD7 binding to enhancer elements of genes involved in neuronal differentiation and cell cycle maintenance in the subventricular zone. ..
  58. Regulation of Periostin by TGFbeta and BMP in Heart Development
    Thomas Doetschman; Fiscal Year: 2010
    ..These proposed studies will largely focus on determining what role the secreted extracellular matrix protein Periostin (POSTN) plays during valve matrix remodeling;and the part that TGF2 and BMP signaling plays in regulating POSTN. ..
  59. MR and BL Tracking of Stem/Progenitor Cells in the CNS
    Jeff W Bulte; Fiscal Year: 2013
    ..The ultimate goal is to develop ways of reporting on successful cell transplantation without removing any tissue, and to provide neurologists with a means to evaluate stem cell treatment in their patients. ..
  60. Systematic functional analysis of non-coding sequences in transgenic zebrafish
    Shannon Fisher; Fiscal Year: 2010
    ..Through application of this approach, we aim to establish a paradigm and generate reagents that will aid our understanding of the wealth of information arising out of genome sequencing efforts. ..
  61. Discovering More Juvenile Myoclonic Epilepsy Genes by a Consortium
    Antonio V Delgado-Escueta; Fiscal Year: 2013
    ..Cures in JME can become a reality only by defining its many genes and then unravelling their epilepsy/disease causing mechanisms. ..
  62. GABA-A Receptor Pharmacology of Neural Coding in Hippocampal Place Cells
    TARA MONIQUE STEWART; Fiscal Year: 2012
    ..abstract_text> ..
    Elizabeth Eklund; Fiscal Year: 2000
    ..The third aim is to investigate the in vivo changes of proteins binding to the gp91phox promoter, an extension of the in vitro footprinting methods that have been utilized to date for this project. ..
    JACK OLSON; Fiscal Year: 1999
    ..This research may point to ODC as an obligatory link in the integration of this pathway and thus in the vascular pathogenesis of many diseases, including cancer, atherosclerosis, restenosis after angioplasty, and hypertension. ..
    Huntington Potter; Fiscal Year: 1990
    CLIFFORD BOGUE; Fiscal Year: 2000
    ..The additional training provided in this proposal will prepare the candidate for an independent and productive career in the biology of lung development. ..
    ANN FEENEY; Fiscal Year: 2000
    ..These studies will hopefully elucidate the role(s) of these VH flanking MARs, and will determine which stage(s) and which function(s) in B cell differentiation are affected by them. ..
    ARNOLD RIZZINO; Fiscal Year: 2005
    ..The last studies are highly significant in their own right, because they will provide novel insights into the mechanisms used by members of two large transcription factor families to stimulate transcription. ..
    Susan Berget; Fiscal Year: 2002
    ..The overall goal of these experiments is to understand how an enhancer resembling an exon participates in both polyadenylation and splicing of neighboring exons. ..
  70. Dissecting the functions of a novel cis element Promoter Targeting Sequence
    Jumin Zhou; Fiscal Year: 2007
    ..abstract_text> ..
    Gary Nabel; Fiscal Year: 1993
    ..The goal of this research is to understand the heterogeneity of kappaB binding proteins, to define cDNAs encoding them, and to selectively inhibit their action on the HIV enhancer without affecting cell viability and function...