Summary: The early developmental stage of MAMMALS. It generally defines the period from cleavage into two BLASTOMERES to the end of embryonic differentiation of basic structures. In the human, the embryo represents the first two months of intrauterine development preceding the stages of the FETUS.

Top Publications

  1. pmc Surface antigens of human embryonic stem cells: changes upon differentiation in culture
    Jonathan S Draper
    Department of Biomedical Science, University of Sheffield, UK
    J Anat 200:249-58. 2002
  2. ncbi Regulation of redox metabolism in the mouse oocyte and embryo
    Remi Dumollard
    Laboratoire de Biologie du Développement UMR 7009 CNRS Paris VI, Observatoire, Station Zoologique, Villefranche sur Mer, France
    Development 134:455-65. 2007
  3. ncbi Non-invasive amino acid turnover predicts human embryo developmental capacity
    Franchesca D Houghton
    Department of Biology, University of York, PO Box 373, York YO10 5YW, UK
    Hum Reprod 17:999-1005. 2002
  4. ncbi Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells
    Mark Richards
    Department of Obstetrics and Gynecology, National University of Singapore, Kent Ridge, Singapore
    Stem Cells 21:546-56. 2003
  5. ncbi Differences between human and mouse embryonic stem cells
    Irene Ginis
    Stem Cell Section, Laboratory of Neurosciences, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Dev Biol 269:360-80. 2004
  6. pmc LPA3-mediated lysophosphatidic acid signalling in embryo implantation and spacing
    Xiaoqin Ye
    Department of Molecular Biology, Helen L Dorris Child and Adolescent Neuropsychiatric Disorder Institute, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 435:104-8. 2005
  7. ncbi Eight-hydroxy-2'-deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos in an in vitro fertilization-embryo transfer program
    Tomofumi Seino
    Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, Japan
    Fertil Steril 77:1184-90. 2002
  8. ncbi Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo
    Trista E North
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Immunity 16:661-72. 2002
  9. ncbi The homeodomain protein Meis1 is essential for definitive hematopoiesis and vascular patterning in the mouse embryo
    Valeria Azcoitia
    Departamento de Inmunologia y Oncologia, Centro Nacional de Biotecnología CSIC UAM, Campus de Cantoblanco, Madrid, Spain
    Dev Biol 280:307-20. 2005
  10. ncbi Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF
    L J McKenzie
    Department of Obstetrics, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Reprod 19:2869-74. 2004

Detail Information

Publications406 found, 100 shown here

  1. pmc Surface antigens of human embryonic stem cells: changes upon differentiation in culture
    Jonathan S Draper
    Department of Biomedical Science, University of Sheffield, UK
    J Anat 200:249-58. 2002
    ..In all of these features the human ES cells, and their pattern of differentiation, resembled the pluripotent human EC cell line NTERA-2 although clearly the range of cells generated by the hES cells was considerably greater...
  2. ncbi Regulation of redox metabolism in the mouse oocyte and embryo
    Remi Dumollard
    Laboratoire de Biologie du Développement UMR 7009 CNRS Paris VI, Observatoire, Station Zoologique, Villefranche sur Mer, France
    Development 134:455-65. 2007
    ..It has been known for some time that the early mouse embryo metabolises external pyruvate and lactate but not glucose to produce ATP...
  3. ncbi Non-invasive amino acid turnover predicts human embryo developmental capacity
    Franchesca D Houghton
    Department of Biology, University of York, PO Box 373, York YO10 5YW, UK
    Hum Reprod 17:999-1005. 2002
    ..These problems could be overcome if single embryos of known developmental competence could be selected for transfer on day 2/3 of development, but current methods, which rely on morphological appearance, are poor predictors of viability...
  4. ncbi Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells
    Mark Richards
    Department of Obstetrics and Gynecology, National University of Singapore, Kent Ridge, Singapore
    Stem Cells 21:546-56. 2003
    ..Also, differences in feeder support exist between human cell types and sources. The use of human adult skin feeders is convenient for hES cell support given the ease of obtaining skin biopsies...
  5. ncbi Differences between human and mouse embryonic stem cells
    Irene Ginis
    Stem Cell Section, Laboratory of Neurosciences, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Dev Biol 269:360-80. 2004
    ..2) and to feeder-free subclones of H1, H7, and H9, indicating that the observed differences between human and mouse ES cells were species-specific rather than arising from differences in culture conditions...
  6. pmc LPA3-mediated lysophosphatidic acid signalling in embryo implantation and spacing
    Xiaoqin Ye
    Department of Molecular Biology, Helen L Dorris Child and Adolescent Neuropsychiatric Disorder Institute, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 435:104-8. 2005
    Every successful pregnancy requires proper embryo implantation. Low implantation rate is a major problem during infertility treatments using assisted reproductive technologies...
  7. ncbi Eight-hydroxy-2'-deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos in an in vitro fertilization-embryo transfer program
    Tomofumi Seino
    Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, Japan
    Fertil Steril 77:1184-90. 2002
    To determine the effects of oxidative stress on the quality of oocytes and embryos, 8-hydroxy-2'-deoxyguanosine (8-OHdG) in granulosa cells was quantitatively studied during an in vitro fertilization and embryo transfer (IVF-ET) program.
  8. ncbi Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo
    Trista E North
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Immunity 16:661-72. 2002
    ..first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent ..
  9. ncbi The homeodomain protein Meis1 is essential for definitive hematopoiesis and vascular patterning in the mouse embryo
    Valeria Azcoitia
    Departamento de Inmunologia y Oncologia, Centro Nacional de Biotecnología CSIC UAM, Campus de Cantoblanco, Madrid, Spain
    Dev Biol 280:307-20. 2005
    ..These results show a requirement for Meis1 in the establishment of definitive hematopoiesis in the mouse embryo. Meis1 mutant mice also displayed complete agenesis of the megakaryocyte lineage and localized defects in vascular ..
  10. ncbi Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF
    L J McKenzie
    Department of Obstetrics, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Reprod 19:2869-74. 2004
    A biochemical marker for embryo development would increase the chance of a successful pregnancy with IVF by optimizing oocyte and embryo selection, and allow fewer embryos to be transferred...
  11. ncbi Endothelial cells stimulate self-renewal and expand neurogenesis of neural stem cells
    Qin Shen
    Center for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY 12208, USA
    Science 304:1338-40. 2004
    ..Endothelial coculture stimulates neuroepithelial cell contact, activating Notch and Hes 1 to promote self-renewal. These findings identify endothelial cells as a critical component of the neural stem cell niche...
  12. ncbi Embryo donation for medical research: attitudes and concerns of potential donors
    Catherine A McMahon
    Royal North Shore Hospital, Sydney, Australia
    Hum Reprod 18:871-7. 2003
    ..However, studies have shown that few couples with stored embryos opt to donate them for research. Herein, the attitudes and concerns of potential embryo donors to donation of surplus embryos for medical research were examined.
  13. ncbi Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens
    J K Henderson
    Section of Reproductive Biology, The School of Medicine and Biomedical Science and Department of Biomedical Science, Univeristy of Sheffield, Sheffield, UK
    Stem Cells 20:329-37. 2002
    ..cell-surface antigens, SSEA3, SSEA4, TRA-1-60, and TRA-1-81, and by the lack of SSEA1, and that inner cell mass cells of the human blastocyst express a similar antigen profile, in contrast to the corresponding cells of the mouse embryo.
  14. ncbi Mitochondrial DNA in the oocyte and the developing embryo
    Pascale May-Panloup
    INSERM U694, F 49033 Angers, France
    Curr Top Dev Biol 77:51-83. 2007
    ..This chapter discusses recent findings concerning mitochondrial DNA content and its expression during oogenesis, fertilization, and early embryonic development...
  15. ncbi Zygote arrest 1 (Zar1) is a novel maternal-effect gene critical for the oocyte-to-embryo transition
    Xuemei Wu
    Department of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Genet 33:187-91. 2003
    ..Thus, Zar1 is the first identified oocyte-specific maternal-effect gene that functions at the oocyte-to-embryo transition and, as such, offers new insights into the initiation of embryonic development and fertility control in ..
  16. ncbi Human embryonic stem cells and their differentiated derivatives are less susceptible to immune rejection than adult cells
    Micha Drukker
    Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University, 91904 Jerusalem, Israel
    Stem Cells 24:221-9. 2006
    ..Thus, immunosuppressive regimens for hESC-based therapeutics could be highly reduced compared with conventional organ transplantation because direct allorejection processes of hESCs and their derivatives are considerably weaker...
  17. ncbi Nodal antagonists regulate formation of the anteroposterior axis of the mouse embryo
    Masamichi Yamamoto
    Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation JST, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    Nature 428:387-92. 2004
    Patterning of the mouse embryo along the anteroposterior axis during body plan development requires migration of the distal visceral endoderm (DVE) towards the future anterior side by a mechanism that has remained unknown...
  18. ncbi A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development
    Elizabeth M Ferguson
    Department of Biology, University of York, York, United Kingdom
    Mol Reprod Dev 73:1195-201. 2006
    ..The development of zygotes exposed to MP during oocyte maturation, and of zygotes exposed to MP during embryo culture has been assessed in terms of oxygen consumption by oocytes and embryos during a 4-6 hr incubation period ..
  19. ncbi Wagging the dogma; tissue-specific cell cycle control in the mouse embryo
    Michele Pagano
    Department of Pathology and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA
    Cell 118:535-8. 2004
  20. ncbi Systems biology of the 2-cell mouse embryo
    A V Evsikov
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    Cytogenet Genome Res 105:240-50. 2004
    The transcriptome of the 2-cell mouse embryo was analyzed to provide insight into the molecular networks at play during nuclear reprogramming and embryonic genome activation...
  21. ncbi Long-term culture of human embryonic stem cells in feeder-free conditions
    Elen S Rosler
    Geron Corporation, Menlo Park, California, USA
    Dev Dyn 229:259-74. 2004
  22. ncbi A germ cell origin of embryonic stem cells?
    Thomas P Zwaka
    University of Wisconsin Medical School and The National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
    Development 132:227-33. 2005
    ..Here, we review recent evidence that the closest in vivo equivalent of an ES cell is an early germ cell...
  23. ncbi Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting
    Masahiro Kaneda
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 429:900-3. 2004
    ..These results indicate that both Dnmt3a and Dnmt3L are required for methylation of most imprinted loci in germ cells, but also suggest the involvement of other factors...
  24. pmc GATA4 is essential for formation of the proepicardium and regulates cardiogenesis
    Alistair J Watt
    Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53202
    Proc Natl Acad Sci U S A 101:12573-8. 2004
    ..We have used tetraploid embryo complementation to rescue these defects and generated clonal embryonic day 9...
  25. pmc Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation
    Lorenzo F Sempere
    Department of Genetics, Dartmouth Medical School, Hanover, NH 03755, USA
    Genome Biol 5:R13. 2004
    ..lin-4, let-7 and other miRNA genes are conserved in mammals, and their potential functions in mammalian development are under active study...
  26. ncbi Expression of CD41 marks the initiation of definitive hematopoiesis in the mouse embryo
    Hanna K A Mikkola
    Department of Hematology Oncology, Children s Hospital, Boston, MA, USA
    Blood 101:508-16. 2003
    ..In the mouse embryo, expression of CD41 was detected in yolk sac blood islands and in fetal liver...
  27. ncbi Methylation dynamics in the early mammalian embryo: implications of genome reprogramming defects for development
    T Haaf
    Johannes Gutenberg Universitat Mainz, Germany
    Curr Top Microbiol Immunol 310:13-22. 2006
    ..whereas the maternal genome is passively demethylated by a replication-dependent mechanism after the two-cell embryo stage...
  28. ncbi Endothelial and hematopoietic cell fate of human embryonic stem cells originates from primitive endothelium with hemangioblastic properties
    Lisheng Wang
    Robarts Research Institute, Krembil Centre for Stem Cell Biology and Regenerative Medicine, 100 Perth Drive, London, Ontario N6A 5K8, Canada
    Immunity 21:31-41. 2004
  29. ncbi Induction of Notch signaling by tumor necrosis factor in rheumatoid synovial fibroblasts
    Kiichiro Ando
    Department of Molecular Genetics, Nagoya City University Medical School, 1 Kawasumi, Mizuho cho, Mizuho ku, Nagoya 467 8601, Japan
    Oncogene 22:7796-803. 2003
    ..Notch-1, Notch-4, and Jagged-2 proteins were also detected in the developing synovium of mouse embryo. Thus, RSF may have re-acquired the primordial phenotype, accounting for the hyperproliferation and aggressive ..
  30. ncbi Genomic alterations in cultured human embryonic stem cells
    Anirban Maitra
    McKusick Nathans Institute of Genetic Medicine, Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Genet 37:1099-103. 2005
  31. ncbi Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
    Naohiro Terada
    Department of Pathology, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    Nature 416:542-5. 2002
    ..Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation...
  32. ncbi Embryo morphology and development are dependent on the chromosomal complement
    M Cristina Magli
    Reproductive Medicine Unit, Società Italiana di Studi di Medicina della Riproduzione, Bologna, Italy
    Fertil Steril 87:534-41. 2007
    To analyze embryo morphology in relation to the corresponding chromosomal status, in order to evaluate the effects of aneuploidy over fragmentation, delayed cleavage, and arrested cleavage.
  33. ncbi High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors
    Yue Ma
    National Primate Research Center, School of Medicine, University of Wisconsin, Madison, Wisconsin, USA
    Stem Cells 21:111-7. 2003
    ..These results also have important implications for the possible future use of gene-modified human ES cells in transplantation and tissue regeneration applications...
  34. ncbi The transcription factor NFIA controls the onset of gliogenesis in the developing spinal cord
    Benjamin Deneen
    Division of Biology 216 76, California Institute of Technology, 1201 East California Boulevard, Pasadena, California 91125, USA
    Neuron 52:953-68. 2006
    ..At later stages, NFIA promotes migration and differentiation of astrocyte precursors, a function that is antagonized in oligodendrocyte precursors by Olig2...
  35. ncbi Determination of left-right patterning of the mouse embryo by artificial nodal flow
    Shigenori Nonaka
    Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    Nature 418:96-9. 2002
    ..The artificial flow was also able to direct the situs of mutant mouse embryos with immotile cilia. These results provide the first direct evidence for the role of mechanical fluid flow in L-R patterning...
  36. ncbi Mullerian inhibiting substance levels at the time of HCG administration in IVF cycles predict both ovarian reserve and embryo morphology
    T Silberstein
    Women and Infants Hospital of Rhode Island, Brown University Division of Biology and Medicine, Providence, Rhode Island 02905, USA
    Hum Reprod 21:159-63. 2006
    ..known about how ovarian reserve affects oocyte quality, we attempt here to assess the predictive value of MIS on embryo morphology and IVF outcome...
  37. ncbi CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo
    Michael J Ferkowicz
    Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Development 130:4393-403. 2003
    ..Thus, CD41 expression marks the onset of primitive and definitive hematopoiesis in the murine embryo and persists as a marker of some stem and progenitor cell populations in the fetal liver and adult marrow, ..
  38. ncbi Head-tail patterning of the vertebrate embryo: one, two or many unresolved problems?
    Claudio D Stern
    European Union Network of Excellence Cells into Organs, University College London, UK
    Int J Dev Biol 50:3-15. 2006
    When, where and how is the head-tail axis of the embryo set up during development? These are such fundamental and intensely studied questions that one might expect them to have been answered long ago...
  39. ncbi Wnt5a and Wnt5b exhibit distinct activities in coordinating chondrocyte proliferation and differentiation
    Yingzi Yang
    Genetic Disease Research Branch, National Human Genome Research Institute, National Institute of Health, Bethesda, MD 20892, USA
    Development 130:1003-15. 2003
    ..Our data indicate that Wnt5a and Wnt5b control the pace of transitions between different chondrocyte zones...
  40. ncbi Contribution of the oocyte to embryo quality
    Marc Andre Sirard
    Centre de Recherche en Biologie de la Reproduction, Department of Animal Sciences, Laval University, Pav Comtois, Sainte Foy, Que, Canada G1K 7P4
    Theriogenology 65:126-36. 2006
    The ability of a bovine embryo to develop to the blastocyst stage, to implant and to generate a healthy offspring is not a simple process...
  41. ncbi The role of mitochondrial function in the oocyte and embryo
    Remi Dumollard
    Department of Physiology, University College London, London WC1E 6BT, United Kingdom
    Curr Top Dev Biol 77:21-49. 2007
    ..The localization of mitochondria in the egg during maturation and their segregation to blastomeres in the cleaving embryo are strictly regulated...
  42. ncbi Retinoic acid controls the bilateral symmetry of somite formation in the mouse embryo
    Julien Vermot
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP College de France, BP 10142, 67404 Illkirch Cedex, Strasbourg, France
    Science 308:563-6. 2005
    ..These data implicate retinoic acid as an endogenous signal that maintains the bilateral synchrony of mesoderm segmentation, and therefore controls bilateral symmetry, in vertebrate embryos...
  43. pmc An aneuploid mouse strain carrying human chromosome 21 with Down syndrome phenotypes
    Aideen O'Doherty
    Department of Neurodegenerative Disease, Institute of Neurology, Queen Square, London WC1N 3BG, UK
    Science 309:2033-7. 2005
    ..Transchromosomic mouse lines such as Tc1 may represent useful genetic tools for dissecting other human aneuploidies...
  44. ncbi Definitive hematopoiesis requires the mixed-lineage leukemia gene
    Patricia Ernst
    Howard Hughes Medical Institute, Dana Farber Cancer Institute, Harvard Medical School, Departments of Pathology and Medicine, Harvard Medical School, Boston, MA 02115 USA
    Dev Cell 6:437-43. 2004
    ..These results demonstrate an intrinsic requirement for MLL in definitive hematopoiesis, where it is essential for the generation of HSCs in the embryo.
  45. ncbi Redox regulation of the G1 to S phase transition in the mouse embryo fibroblast cell cycle
    Sarita G Menon
    Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City 52242, USA
    Cancer Res 63:2109-17. 2003
  46. ncbi Nanog: a new recruit to the embryonic stem cell orchestra
    Fatima Cavaleri
    University of Pennsylvania, School of Veterinary Medicine, Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, 382 West Street Road, Kennett Square, PA 19348, USA
    Cell 113:551-2. 2003
    ..Nanog, a homeobox transcription factor, plays a crucial role in the second embryonic cell fate specification following formation of the blastocyst...
  47. pmc Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model
    Lars M Bjorklund
    Udall Parkinson s Disease Research Center of Excellence, Neuroregeneration Laboratories, and Molecular Neurobiology Laboratory, McLean Hospital Harvard Medical School, 115 Mill Street, Belmont, MA 02478, USA
    Proc Natl Acad Sci U S A 99:2344-9. 2002
    ..These results demonstrate that transplanted ES cells can develop spontaneously into DA neurons. Such DA neurons can restore cerebral function and behavior in an animal model of Parkinson's disease...
  48. pmc Rapid generation of inducible mouse mutants
    Jost Seibler
    Artemis Pharmaceuticals GmbH, Neurather Ring 1, 51063 Cologne, Germany
    Nucleic Acids Res 31:e12. 2003
    ..Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol...
  49. ncbi Overlapping expression patterns of the multiligand endocytic receptors cubilin and megalin in the CNS, sensory organs and developing epithelia of the rodent embryo
    Emeline Assemat
    Inserm UMR 538, Faculte de Medecine Saint Antoine, Universite Pierre et Marie Curie, 27 rue de Chaligny, 75012 Paris, France
    Gene Expr Patterns 6:69-78. 2005
    ..anti-cubilin antibodies induces fetal resorptions or cranio-facial malformations their function in the developing embryo remains unclear...
  50. ncbi Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease
    Jong Hoon Kim
    Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 418:50-6. 2002
    ..The dopamine neurons generated by these stem cells show electrophysiological and behavioural properties expected of neurons from the midbrain. Our results encourage the use of ES cells in cell-replacement therapy for Parkinson's disease...
  51. ncbi Involvement of SIP1 in positioning of somite boundaries in the mouse embryo
    Mitsuji Maruhashi
    Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
    Dev Dyn 234:332-8. 2005
    ..The phenotype of Sip1-/- embryo introduces a new molecular component SIP1 in positioning of somite boundaries, and provides support for the ..
  52. ncbi No evidence for infection of human embryonic stem cells by feeder cell-derived murine leukemia viruses
    Michal Amit
    Department of Obstetrics and Gynecology, Rambam Medical Center, Faculty of Medicine, The Technion, Haifa, Israel
    Stem Cells 23:761-71. 2005
    ..Our results suggest that recently established hESC lines are free of MuLV infections despite long-term close contact with MEFs...
  53. ncbi Capacity of adult and prepubertal mouse oocytes to undergo embryo development in the presence of cysteamine
    D G De Matos
    Dutch Speaking Brussels Free University, Laarbeeklaan 101, 1090 Brussels, Belgium
    Mol Reprod Dev 64:214-8. 2003
    ..effect of stimulating GSH synthesis during in vitro maturation (IVM) of adult and prepubertal mouse oocytes on the embryo developmental rate...
  54. ncbi Developmental potential and behavior of tetraploid cells in the mouse embryo
    Guy S Eakin
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Dev Biol 288:150-9. 2005
    ..These findings suggest that the results of previously published tetraploid complementation assays may be influenced by the presence of tetraploid cells in the otherwise diploid embryonic regions...
  55. ncbi MRI analysis of angiogenesis during mouse embryo implantation
    Vicki Plaks
    Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
    Magn Reson Med 55:1013-22. 2006
    Uterine receptivity and embryo implantation depend on local induction of angiogenesis and vascular permeability...
  56. ncbi Role of the EGF-CFC gene cripto in cell differentiation and embryo development
    Gabriella Minchiotti
    International Institute of Genetics and Biophysics, CNR, Via Marconi 12, 80125 Naples, Italy
    Gene 287:33-7. 2002
    ..Here we review the biological role of cripto in development and differentiation, pointing out recent findings on the biochemical interactions of Cripto, Nodal and Activin-like receptors...
  57. pmc Distinct roles for ADAM10 and ADAM17 in ectodomain shedding of six EGFR ligands
    Umut Sahin
    Cell Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, Box 368, 1275 York Avenue, New York, NY 10021, USA
    J Cell Biol 164:769-79. 2004
    ..Identification of EGFR ligand sheddases is a crucial step toward understanding the mechanism underlying ectodomain release, and has implications for designing novel inhibitors of EGFR-dependent tumors...
  58. ncbi Current status of embryo technologies in sheep and goat
    Y Cognie
    INRA, Physiologie de la Reproduction et des Comportements, 37380 Nouzilly, France
    Theriogenology 59:171-88. 2003
    This review presents an overview of the technical bases of in vivo and in vitro embryo production in sheep and goat...
  59. ncbi Culture of zygotes increases TRP53 [corrected] expression in B6 mouse embryos, which reduces embryo viability
    A Li
    Human Reproduction Unit, Disciplines of Physiology and Medicine, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Biol Reprod 76:362-7. 2007
    ..001). This shift in viability did not occur when embryos were only subjected to 24 h of culture from the compacted embryo stage...
  60. ncbi Adamts9 is widely expressed during mouse embryo development
    Katherine A Jungers
    Department of Biomedical Engineering and Orthopaedic Research Center, Lerner Research Institute, Cleveland Clinic Foundation ND20, OH 44195, USA
    Gene Expr Patterns 5:609-17. 2005
  61. ncbi An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells
    Petra Stojkovic
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK
    Stem Cells 23:306-14. 2005
    ..Feeder cells derived from hESCs offers a potentially more secure autogeneic and genotypically homogenous system for the growth of undifferentiated hESCs...
  62. ncbi Gene expression and developmental competence of bovine embryos produced in vitro under varying embryo density conditions
    Alexandre Tavares Duarte de Oliveira
    Embryology and Biotechnology of Reproduction Laboratory, Faculty of Veterinary Medicine, UFRGS, Cx Postal 15004, 91501 970 Porto Alegre, RS, Brazil
    Theriogenology 64:1559-72. 2005
    The objective of this study was to determine the effects of oocyte/embryo density (embryo number:medium volume ratio) on developmental competence and relative transcription of developmentally important genes in in vitro-produced bovine ..
  63. pmc GenePaint.org: an atlas of gene expression patterns in the mouse embryo
    Axel Visel
    Max Planck Institute of Experimental Endocrinology, Feodor Lynen Strasse 7, D 30625 Hannover, Germany
    Nucleic Acids Res 32:D552-6. 2004
    ..A virtual microscope tool, the Zoom Image Server, was implemented in GenePaint.org and permits interactive zooming and panning across approximately 15,000 high-resolution images...
  64. ncbi A prediction model for selecting patients undergoing in vitro fertilization for elective single embryo transfer
    Claudine C Hunault
    Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Erasmus University Medical Center, The, Rotterdam, Netherlands
    Fertil Steril 77:725-32. 2002
    ..Construction of a prediction model to enable the selection of patients for elective single ET...
  65. pmc DNA damage response and Ku80 function in the vertebrate embryo
    Catherine L Bladen
    Institute of Molecular Medicine and Genetics, Medical College of Georgia Augusta, GA 30912, USA
    Nucleic Acids Res 33:3002-10. 2005
    ..Here we describe use of the zebrafish embryo as a model system to identify determinants of the in vivo response to ionizing radiation-induced DNA damage...
  66. ncbi BMP receptor IA is required in the mammalian embryo for endodermal morphogenesis and ectodermal patterning
    Shannon Davis
    Department of Cell Biology, Duke University Medical Center, Durham NC 27710, USA
    Dev Biol 270:47-63. 2004
    ..Our results reveal critical roles for BMP signaling in endodermal morphogenesis and ectodermal patterning...
  67. ncbi Cyclooxygenase-2-derived endogenous prostacyclin enhances mouse embryo hatching
    Jaou Chen Huang
    Department of Obstetrics and Gynecology, University of Texas Health Science Center Houston, Houston, TX 77030, USA
    Hum Reprod 19:2900-6. 2004
    The role of prostaglandins (PGs) in embryo hatching remains controversial. In addition, there is no direct evidence that mouse embryos synthesize PGs.
  68. pmc Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors
    Jamie M Sperger
    Wisconsin National Primate Research Center and Department of Anatomy, School of Medicine, University of Wisconsin, Madison, WI 53715, USA
    Proc Natl Acad Sci U S A 100:13350-5. 2003
    ..These genes are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype...
  69. ncbi Post-insemination milk progesterone concentration and embryo survival in dairy cows
    A J H Stronge
    Animal Reproduction Department, Teagasc Research Centre, Athenry, CO, Galway, Ireland
    Theriogenology 64:1212-24. 2005
    ..analysis was used to evaluate the relationship between post-insemination milk progesterone concentration and embryo survival, and between milk yield and milk progesterone concentration...
  70. pmc Spatial codes in dendritic BC1 RNA
    Ilham A Muslimov
    Department of Physiology and Pharmacology, The Robert F Furchgott Center for Neural and Behavioral Science, State University of New York Health Science Center at Brooklyn, Brooklyn, NY 11203, USA
    J Cell Biol 175:427-39. 2006
    ..Our work suggests that a BC1 KT motif encodes distal targeting via the A2 pathway and that architectural RNA elements, such as KT motifs, may function as spatial codes in neural cells...
  71. pmc Phenotypic analysis of Meltrin alpha (ADAM12)-deficient mice: involvement of Meltrin alpha in adipogenesis and myogenesis
    Tomohiro Kurisaki
    Field of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Japan
    Mol Cell Biol 23:55-61. 2003
    ..We also report here the chromosomal locations of Meltrin alpha in the mouse and rat...
  72. ncbi Associations between milk progesterone concentration on different days and with embryo survival during the early luteal phase in dairy cows
    R E McNeill
    Animal Reproduction Department, Teagasc Research Centre, Athenry, Co Galway, Ireland
    Theriogenology 65:1435-41. 2006
    The relationships between the concentration of milk progesterone and early embryo survival on Days 4-8 inclusive and between the concentration of progesterone on different days from Days 0-8 inclusive following ovulation and insemination ..
  73. ncbi Differential expression and activation of Stat3 during mouse embryo implantation and decidualization
    Chun Bo Teng
    College of Life Sciences, Northeast Agricultural University, Harbin, China
    Mol Reprod Dev 69:1-10. 2004
    ..Stat3 is specifically activated during mouse embryo implantation...
  74. ncbi Expression of neurodevelopmental markers by cultured porcine neural precursor cells
    Philip H Schwartz
    National Human Neural Stem Cell Resource, Children s Hospital of Orange County, Research Institute, 455 South Main Street, Orange, California 92868 3874, USA
    Stem Cells 23:1286-94. 2005
  75. ncbi The two-domain hypothesis in Beckwith-Wiedemann syndrome: autonomous imprinting of the telomeric domain of the distal chromosome 7 cluster
    Flavia Cerrato
    Dipartimento di Scienze Ambientali, Seconda Universita di Napoli, Via Vivaldi 43, 81100 Caserta, Italy
    Hum Mol Genet 14:503-11. 2005
    ..but not paternal, transmission of the transgene results in fetal growth restriction, suggesting that during evolution the acquisition of imprinting may have been facilitated by the opposite effects of the two domains on embryo growth.
  76. ncbi Effect of sperm glutathione peroxidases 1 and 4 on embryo asymmetry and blastocyst quality in oocyte donation cycles
    Marcos Meseguer
    Instituto Valenciano de Infertilidad Valencia, Universidad de Valencia, Valencia, Spain
    Fertil Steril 86:1376-85. 2006
    ..oxidative glutathione stress system in terms of enzymatic activity and mitochondrial RNA (mRNA) expression on embryo quality and reproductive outcome...
  77. ncbi Regionalised signalling within the extraembryonic ectoderm regulates anterior visceral endoderm positioning in the mouse embryo
    Lucy Richardson
    The Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Mech Dev 123:288-96. 2006
    The development of the anterior-posterior (AP) axis in the mammalian embryo is controlled by interactions between embryonic and extraembryonic tissues...
  78. ncbi Hematopoietic stem cell enrichment from the AGM region of the mouse embryo
    Catherine Robin
    Department of Cell Biology and Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands
    Methods Mol Med 105:257-72. 2005
    ..This review presents the best methods (sorting based on specific antibody staining, transgenic markers, cell cycle) for the purification and isolation of HSCs from the mouse embryo.
  79. ncbi Fate-mapping the mammalian hindbrain: segmental origins of vestibular projection neurons assessed using rhombomere-specific Hoxa2 enhancer elements in the mouse embryo
    Massimo Pasqualetti
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique Inserm Université Louis Pasteur, Unite Mixte de Recherche 7104, Commanaute Urbaine de Strasbourg, 67404 Illkirch Cedex, France
    J Neurosci 27:9670-81. 2007
    ..Comparison with studies in the chicken embryo shows that the rostrocaudal patterning of identified vestibular projection neuron groups is generally well ..
  80. ncbi Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century
    Mario R Capecchi
    Howard Hughes Medical Institute, Department of Human Genetics, University of Utah School of Medicine, 15 North 2030 East, Room 5100, Salt Lake City, Utah 84112 5331, USA
    Nat Rev Genet 6:507-12. 2005
    ..Here I provide a personal account of the work that led to the generation of gene targeting which now lies at the centre of functional genomic analysis...
  81. ncbi Embryo quality and production efficiency of porcine parthenotes is improved by phytohemagglutinin
    Mukesh Kumar Gupta
    Department of Bioscience and Biotechnology, Bio Organ Research Center, Konkuk University, Seoul, South Korea
    Mol Reprod Dev 74:435-44. 2007
    ..This study was therefore, designed to investigate the effect of PHA on the preimplantation embryo development and quality of in vitro produced porcine parthenotes...
  82. ncbi Whole insect and mammalian embryo imaging with confocal microscopy: morphology and apoptosis
    Robert M Zucker
    Division of Reproductive Toxicology, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry A 69:1143-52. 2006
    ..LT has been shown to be an indicator of apoptotic cell death which is correlated to other standard apoptotic assays...
  83. ncbi Transglutaminase 3 expression in C57BL/6J mouse embryo epidermis and the correlation with its differentiation
    Jian Zhang
    National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
    Cell Res 15:105-10. 2005
    ..In the present study, we detected the expression of TGM3 in the mouse embryo using RT-PCR. TGM3 mRNA is weakly presented from E11.5 to E14.5 and increases significantly from E15.5 to birth...
  84. ncbi Embryo-uterine interactions via the neuregulin family of growth factors during implantation in the mouse
    Naoko Brown
    Division of Reproductive and Developmental Biology, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2370, USA
    Biol Reprod 71:2003-11. 2004
    ..cell-signaling molecules with recognized roles in cancer and development, but little is known about their role in embryo implantation...
  85. ncbi Genomic imprinting contributes to thyroid hormone metabolism in the mouse embryo
    Chen En Tsai
    Department of Anatomy, University of Cambridge, Downing Street, United Kingdom
    Curr Biol 12:1221-6. 2002
    ..These results demonstrate that disruption of the imprinting status of Dio3 results in abnormal thyroid hormone levels and may contribute to the phenotypic abnormalities in UPD12 mice and UPD14 humans...
  86. ncbi Zygote donor nitrogen metabolism and in vitro embryo culture perturbs in utero development and IGF2R expression in ovine fetal tissues
    K Powell
    Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
    Theriogenology 66:1901-12. 2006
    ..Elevated plasma urea concentrations in zygote donors were associated with accelerated early embryo development, low pregnancy rates (16%) for embryos from the high N, SOF+ treatment, and significantly influenced ..
  87. ncbi Endometrioma undergoing laparoscopic ovarian cystectomy: its influence on the outcome of in vitro fertilization and embryo transfer (IVF-ET)
    Tao Chuan Loo
    Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Chi Mei Medical Center, Tainan, Taiwan
    J Assist Reprod Genet 22:329-33. 2005
    ..To evaluate the effect of laparoscopic ovarian cystectomy for endometrioma on the clinical outcome of IVF treatment...
  88. ncbi Differentiation of human embryonic stem cells into hematopoietic cells by coculture with human fetal liver cells recapitulates the globin switch that occurs early in development
    Caihong Qiu
    Einstein Center for Human Embryonic Stem Cell Research, Department of Medicine, Division of Hematology and Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA
    Exp Hematol 33:1450-8. 2005
    ..To find a human cell line that could support differentiation of human embryonic stem cells (hESCs) into hematopoietic cells. To determine in detail the expression profiles of the beta-like globin genes in hESC-derived erythroid cells...
  89. pmc Endometriosis and infertility: a multi-cytokine imbalance versus ovulation, fertilization and early embryo development
    S Vassiliadis
    Department of Biology, University of Crete, Heraklion, Crete, Greece
    Clin Dev Immunol 12:125-9. 2005
    ..Thus, regulating the levels of interferon-gamma and tumor necrosis-alpha will facilitate ovulation and fertilization, whereas adjusting the levels of interleukin-1beta and colony stimulating gactor-1 will facilitate implantation...
  90. pmc Gene expression profiling by DNA microarray analysis in mouse embryonic fibroblasts transformed by rasV12 mutated protein and the E1A oncogene
    Sophie Vasseur
    Centre de Recherche INSERM EMI 0116, 163 Avenue de Luminy, BP172, 13009 Marseille, France
    Mol Cancer 2:19. 2003
    ..We used a systematic approach, based on DNA microarray technology to establish a first catalog of genes whose expression is altered by ras and, as such, potentially involved in the regulation of cell growth and transformation...
  91. ncbi Regulatory networks in embryo-derived pluripotent stem cells
    Michele Boiani
    Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Mendelstrasse 7 Von Esmarch Strasse 56, 48149 Munster, Germany
    Nat Rev Mol Cell Biol 6:872-84. 2005
    ..Investigation of the regulatory networks that are responsible for pluripotency in embryo-derived stem cells is fundamental to understanding mammalian development and realizing therapeutic potential...
  92. ncbi Donor oocyte dysmorphisms and their influence on fertilization and embryo quality
    Jorge Ten
    Department of Biology of Reproduction, Instituto Bernabeu, Alicante 03016, Spain
    Reprod Biomed Online 14:40-8. 2007
    The aim of this study was to identify predictors of fertilization rate (FR) and embryo quality (EQ) in 126 donor women who underwent 160 intracytoplasmic sperm injection (ICSI) cycles...
  93. ncbi Smad3 mediates the TGF-beta-induced contraction of type I collagen gels by mouse embryo fibroblasts
    Xiangde Liu
    Pulmonary and Critical Care Medicine, University of Nebraska Medical Center, Omaha, NE 68198 5125, USA
    Cell Motil Cytoskeleton 54:248-53. 2003
    ..To investigate the importance of Smad2 or Smad3 in this augmentation process, embryo-derived fibroblasts from mice lacking expression of Smad2 or Smad3 genes were cast into native type I collagen ..
  94. ncbi Acquisition of Hox codes during gastrulation and axial elongation in the mouse embryo
    Sylvie Forlani
    Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Development 130:3807-19. 2003
  95. ncbi The control of cell motility and epithelial morphogenesis by Jun kinases
    Ying Xia
    Center for Environmental Genetics and Department of Environmental Health, University of Cincinnati Medical Center, 123 East Shields Street, Cincinnati, OH 45267 0056, USA
    Trends Cell Biol 14:94-101. 2004
    ..This article focuses on recent advances in understanding the role of JNK pathway in the regulation of cell migration, cytoskeleton rearrangement and the morphogenesis of epithelial sheets...
  96. ncbi Energy metabolism in pig oocytes and early embryos
    R G Sturmey
    Department of Biology Area 3, University of York, PO Box 373, York YO10 5YW, UK
    Reproduction 126:197-204. 2003
    ..the hypothesis that endogenous triglyceride acts as a metabolic substrate during in vitro maturation and early embryo development was tested...
  97. ncbi Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei
    Alex Bortvin
    Howard Hughes Medical Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
    Development 130:1673-80. 2003
    ..These observations suggest that failure to reactivate the full spectrum of these Oct4-related genes may contribute to embryonic lethality in somatic-cell clones...
  98. ncbi Anti-Müllerian hormone concentrations in the follicular fluid of the preovulatory follicle are predictive of the implantation potential of the ensuing embryo obtained by in vitro fertilization
    Renato Fanchin
    Department of Obstetrics and Gynecology and Reproductive Medicine, Hopital Antoine Beclere, 157 rue de la Porte de Trivaux, 92141 Clamart, France
    J Clin Endocrinol Metab 92:1796-802. 2007
    ..Yet, it still is unclear whether the aptitude of an individual follicle to produce AMH reflects its reproductive competence...
  99. ncbi Phosphatidylinositol 3-kinase activity is critical for glucose metabolism and embryo survival in murine blastocysts
    Joan K Riley
    Department of Obstetrics and Gynecology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Biol Chem 281:6010-9. 2006
    ..To determine the impact of PI3K inhibition on pregnancy outcome, embryo transfer experiments were performed...
  100. pmc Optical projection tomography as a new tool for studying embryo anatomy
    James Sharpe
    Developmental Genetics, MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK
    J Anat 202:175-81. 2003
    ..This becomes particularly important for the visualization of the 3D shapes of specific organs and tissues as it allows the computer to automatically determine the outline of the desired structure...
  101. pmc Mice lacking the metalloprotease-disintegrin MDC9 (ADAM9) have no evident major abnormalities during development or adult life
    Gisela Weskamp
    Cellular Biochemistry and Biophysics Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell Biol 22:1537-44. 2002

Research Grants123 found, 100 shown here

    Keri Hammel; Fiscal Year: 2013
    ..closure of a normally-occurring gap in the eye (the optic fissure) after the fifth week of life in a human embryo. There have been studies of families in which more than one person has been affected by this disorder...
    Keri Hammel; Fiscal Year: 2012
    ..closure of a normally-occurring gap in the eye (the optic fissure) after the fifth week of life in a human embryo. There have been studies of families in which more than one person has been affected by this disorder...
  3. NIH Director's Pioneer Award
    ERICH JARVIS; Fiscal Year: 2009
    ..Repairing the pathway in vocal learners, when damaged, would have profound impact for correcting neurological disorders of speech. ..
  4. Transcription factors regulating sensory gene expression and pain pathways
    Eric E Turner; Fiscal Year: 2011
    ..Together these studies will significantly advance our understanding of the molecular regulation of pain receptor pathways, which may form the basis for future pain treatments. ..
  5. Role of NGF in inflammatory and proliferative cascades of psoriatic disease
    Siba P Raychaudhuri; Fiscal Year: 2012
    ..NGF is a chemical mainly helps in to grow nerves in the embryo. NGF also irritates skin and other human body parts;in scientific language this is called inflammation...
  6. Epigenetic mechanisms relevant to the pathogenesis of ALS
    Neil W Kowall; Fiscal Year: 2013
    ..This transgenic mouse is produced by adding the abnormal human gene that causes ALS to the mouse embryo. These mice develop progressive weakness and die prematurely at about 4 months of age...
    RUTH M STEWARD; Fiscal Year: 2010
    ..We are aiming at developing a PCR approach to differentiate between the forms of PDCD2 found in normal bone marrow and blood, and the PDCD2 forms associated with leukemic cells. ..
  8. Role of the Mammalian Siderophore in Cellular Iron Metabolism and Erythropoiesis
    Laxminarayana R Devireddy; Fiscal Year: 2012
    ..If successful, this grant could help explain the causes of iron deficiency anemia and could help in designing better drugs to combat iron deficiency anemia. ..
    Christina Chambers; Fiscal Year: 2011
    ..The Robert L. Brent Lecture will explore aspects of Radiation Exposure to the Embryo and Child, including aspects of the 60-year follow-up study of radiation exposure in Japan during World War II...
  10. Intracellular Phase Diagram Creation Using a Micro-Differential Scanning Calorime
    GARY L SOLBREKKEN; Fiscal Year: 2010
    ..We will test the new machine on mouse oocytes, mouse and rat embryos, and zebrafish embryos. ..
  11. Glutamate receptor recruitment to new synapses in vivo
    PHILIP ERIC WASHBOURNE; Fiscal Year: 2013
    ..light on whether these proteins and their interactions are required for forming a specific circuit in a developing embryo that is required for a sensorimotor reflex...
  12. Integrated signaling of eicosanoids in HSC formation and regeneration
    Trista E North; Fiscal Year: 2013
    ..The first HSCs arise in the embryo from an area encompassing the dorsal aorta, termed the aorta-gonad mesonephros (AGM) region...
  13. The Role of Decidual Innate Immunity in the Pathogenesis of Preeclampsia
    Se Te Joseph Huang; Fiscal Year: 2012
    ..Perturbation of the balance between defense against pathogens and tolerance of the semi-allogeneic embryo in the decidua contributes to preeclampsia-toxemia (PET), a leading cause of perinatal and maternal morbidity and ..
  14. Transgenerational effects of endocrine disruptors: epigenetics and physiology
    Rebecca A Simmons; Fiscal Year: 2013
    ..pregnancy results in loss of imprinting at two genes critical for development and obesity, Igf2 and Snrpn, in the embryo and placenta...
  15. Mouse models of genomic instability and birth defects
    Michael R Green; Fiscal Year: 2013
    ..A specific objective of the application is to study the mechanism by which tumor suppressor genes become transcriptionally silenced during cancer development. ..
  17. Phosphotyrosine signaling pathways controlling tracheal tube geometry
    KAI G ZINN; Fiscal Year: 2013
    ..We can also analyze tyrosine phosphorylation of the proteins and determine if they physically interact with each other in the embryo.
  18. Novel Zebrafish Models to Study Toxicity of PCBs and Pesticides During Pregnancy
    Lisa Satterwhite; Fiscal Year: 2009
  19. Improving Epigenetic-based Cell Reprogramming with Proteasome Inhibition
    Jaroslaw Staszkiewicz; Fiscal Year: 2011
  20. The Role of MYST Histone Acetyltransferase in Genome Stability
    M Mitchell Smith; Fiscal Year: 2013
    ..Little is known about their range of functions and target pathways. The research proposed in this application is designed to uncover new principles in how these enzymes work, what they do, and how they do it. ..
  21. Pluripotent stem cell-derived HSCs: improvements and molecular mechanisms
    Patrick Cahan; Fiscal Year: 2013
    ..expression profiling of purified HSCs and their immediate precursors at key developmental stages from the mouse embryo (McKinney-Freeman et al 2012)...
  22. RSK-2 regulates integrin-mediated adhesion and migration
    JOE WILLIAM RAMOS; Fiscal Year: 2013
    ..We will also employ mouse embryo fibroblasts and primary culture cells derived from mouse knock-out models...
  23. Role of murine induced pluripotent stem cells on the correction of cardiac and sk
    Diego Fraidenraich; Fiscal Year: 2010
    ..These experiments will help elucidate the molecular mechanisms that the iPS cells may utilize to effect corrections in cardiac and skeletal muscle and will broaden the therapeutic applicability of the iPS cells. ..
  24. Analysis of epigenetic regulation in early mammalian embryos via RNA interference
    Charles R Long; Fiscal Year: 2010
    ..first erased and subsequently re-established during early embryonic development, concomitant with the period of embryo culture following IVF in fertility clinics...
  25. Regulation of Genomic and Epigenomic Stability at CpG Sites
    Alfonso Bellacosa; Fiscal Year: 2013
    ..strain and assess which TDG function is required for ES cell differentiation in vitro and for phenotypes in mouse embryo fibroblasts...
  26. Modifiers of hepcidin expression as new therapies for iron overload
    PAULA GOODMAN FRAENKEL; Fiscal Year: 2013
    ..These studies will lead to the identification of molecules and pathways that may be adapted to generate treatments for patients with thalassemia or hereditary hemochromatosis. ..
  27. Regulation of Rhox5 Transcriptional Elongation
    Marcy E Richardson; Fiscal Year: 2013
    ..The control of these two phenomena will be analyzed in the developmental and tissue-specific program that allows the expression of Rhox5 Pp in the mouse testis. ..
  28. Cooperative Multicenter Reproductive Medicine Network (U10)
    MARCELLE IVONNE CEDARS; Fiscal Year: 2013
    ..activity in oocytes and reduced production of ATP, which limits normal oocyte chromosomal disjunction and embryo development...
  29. Genetic Analysis of FAK Activity
    David D Schlaepfer; Fiscal Year: 2012
    ..Moreover, as FAK-null mouse embryo fibroblasts (MEFs) exhibit both proliferation and motility defects, it remains undetermined whether FAK activity ..
  30. A Cis-regulatory Model for Neural Border Induction
    Daniel Meulemans Medeiros; Fiscal Year: 2013
    ..development, neural crest cells migrate from this neural border domain to form diverse derivatives throughout the embryo, including bones and cartilage in the head, and portions of the cranial ganglia and heart...
  31. Development of a Porcine Model of Atherosclerosis
    Christopher Rogers; Fiscal Year: 2012
    ..Thus, this work will accelerate the discovery of novel therapies for this costly and deadly disease. ..
  32. Graves'Disease Therapy Risks to Mother and Fetus
    Scott A Rivkees; Fiscal Year: 2013
    ..We will define periods of vulnerability of the embryo to affects of PTU and MMI. We will examine the contribution of the hyperthyroid state to birth defects...
  33. Protein Modifications and Unfolded Protein Response in Diabetic Embryopathy
    Zhiyong Zhao; Fiscal Year: 2013
    ..Maternal hyperglycemia disturbs glucose metabolism (glycolysis) in the embryo, augmenting the hexosamine biosynthetic pathway (HBP) to produce high levels of uridine diphosphate N-..
  34. Germ Cell Migration in Drosophila.
    Ruth Lehmann; Fiscal Year: 2012
    ..We use genetic and biochemical approaches, live imaging and in vitro germ cell cultures to address how lipids and lipid modification provide migratory cues and how these signals are interpretated by the migrating germ cells. ..
  35. Validation of Novel Infertility Biomarker
    PETER SUTOVSKY SUTOVSKY; Fiscal Year: 2012
    ..SPTRX3 content will be evaluated against basic measures of treatment outcome, including fertilization and first embryo cleavage rates, incidence of spontaneous abortion following a successful assisted fertilization and embryo ..
    Jacques Robert; Fiscal Year: 2013
    ..We are proposing to continue the maintenance and the development of this unique non-mammalian resource facility for biomedical research for the benefit of the whole scientific community. ..
  37. Vascular Surgery - Estrogen and the Injury Response
    Richard H Karas; Fiscal Year: 2012
    ..studies to date have relied on exploring estrogen effects in mouse models in which an ER is disrupted in the early embryo (whole animal ER knockout mice), so that the ER is deleted from conception in all tissues and cells of the body...
  38. Air Toxics, Neighborhood Environment and Risk of Oral Clefts
    Philip J Lupo; Fiscal Year: 2013
    ..Furthermore, the developing embryo appears to be particularly vulnerable to the effects of HAPs...
  39. Functional Analysis of the Embryonic Epigenome in a Non-rodent Model
    Mark E Westhusin; Fiscal Year: 2013
    ..then decipher the mechanisms by which aberrations in epigenetic reprogramming which occur during preimplantation embryo development result in failed development or disease...
  40. Human Decidual Leukocytes and Their Placental Ligands
    JACK STROMINGER; Fiscal Year: 2009
    ..spiral arteries to increase blood flow to the developing fetus, implantation of the blastocyst (16 cell stage embryo) into the uterus and maternal-fetal tolerance...
  41. Ribosomal Protein Gene Silencing in Neural Crest Stem Cells
    JOHNSON MING YU LIU; Fiscal Year: 2010
    ..that generates most of the bone, cartilage, connective and peripheral nerve tissue in the head in the developing embryo, and abnormalities in the neural crest can lead to craniofacial defects like cleft palate...
  42. In vivo analysis of the mechanisms of axon transport.
    Catherine M Drerup; Fiscal Year: 2011
    ..To address these questions, I will first use the live embryo imaging approach I developed to determine if microtubule dynamics and axon transport of specific cargos are ..
  43. Trophoblast MHC-I: Trigger for Immune-Mediated Rejection of Cloned Bovine Fetuses
    Kenneth L White; Fiscal Year: 2011
  44. Mouse Models of Non-Syndromic Cardiac Progeria
    Ali J Marian; Fiscal Year: 2012
    ..The models could afford the opportunity to delineate the molecular and cellular mechanisms of cardiolaminopathy and shed light into the mechanisms of cardiovascular senescence. ..
  45. Pbx Homeodomain Proteins in Skeletal Muscle Differentiation
    LISA A MAVES; Fiscal Year: 2011
    ..Understanding these mechanisms ultimately may be useful in improving the ability to control muscle precursor differentiation and, therefore, relevant to developing therapies for treating musculoskeletal diseases. ..
    Jeffrey A Simon; Fiscal Year: 2012
    ..This research will advance basic understanding of gene regulatory mechanisms and provide knowledge that could impact stem cell applications in medicine and development of anti-cancer strategies. ..
  47. Experimental engineering of ovarian grafting to promote angiogenesis for fertilit
    GLENN LEWIS SCHATTMAN; Fiscal Year: 2010
    ..tissue banking has been adopted by several thousand patients worldwide because it offers some advantages over embryo/ oocyte banking, especially for children, but tissue grafted back has only generated a few viable pregnancies so ..
  48. Analysis of FIP200 in signal transduction
    Jun Lin Guan; Fiscal Year: 2012
  49. CTGF promotes beta cell proliferation &revascularization in islet transplants
    Uma Gunasekaran; Fiscal Year: 2011
  50. Novel High Affinity and Long-Acting Bovine FSH Analogs for Veterinary Superovulat
    BRUCE DALE WEINTRAUB; Fiscal Year: 2012
  51. Determinants of Disease Expression in Arrhythmogenic Cardiomyopathy
    Jeffrey E Saffitz; Fiscal Year: 2010
    ..This will not only help families with the rare disease, but also people at risk of sudden death due to common forms of heart disease. ..
  52. Generation of Site-Specific Recombinase-Expressing Transgenic Rats using an Enhan
    Eric M Ostertag; Fiscal Year: 2011
    ..The results from this study will generate valuable genetic tools for refined and subtle investigations of gene function in specific tissues. ..
  53. Development of a Porcine Model of Ataxia-Telangiectasia
    Christopher Rogers; Fiscal Year: 2013
    Hannah C Kinney; Fiscal Year: 2010
    ..abstract_text> ..
  55. Effect of preimplantation embryo culture on adult glucose homeostasis
    Paolo Rinaudo; Fiscal Year: 2013
    ..An initial stressor, occurring when the embryo is exquisitely sensitive to environmental changes, reprograms the developing gene networks, tissue, and organs so ..
  56. The Forkhead Transcription Factor, FOXO1, and its Role in Pituitary Gland Develop
    BUFFY SUE ELLSWORTH; Fiscal Year: 2010
    ..Our objective is to determine the requirement of FOXO1 for normal pituitary development by analyzing the pituitary phenotype in mice that lack FOXO1 in the pituitary gland. ..
  57. Germ Cell Migration in Drosophila.
    Ruth Lehmann; Fiscal Year: 2013
    ..Findings resulting from this application are likely to contribute to a better understanding of the cues and cellular processes that guide germ cell migration and that physically distinguish germ cells from other cells in the body. ..
  58. Study on the intracellular Network of TBX3
    Taosheng Huang; Fiscal Year: 2010
    ..demonstrate that TBX3 is overexpressed in breast cancer cell lines, and can immortalize and transform mouse embryo fibroblast cells...
  59. Generation of Human iPS Cells via Non-integrating Vectors
    Wen Shu Wu; Fiscal Year: 2010
    ..Therefore, this is an alternative to the use of human embryonic stem cells for regenerative medicine applications. ..
  60. Role of Cited2 in Hematopoiesis
    Yu Chung Yang; Fiscal Year: 2011
  61. The Effects of Ethanol on Microtubules during Mouse Pre-implantation Development
    Yixian Zheng; Fiscal Year: 2012
    ..deacetylation of 1-tubulin;2) we will Investigate the impact of ethanol on the pre-implantation mouse embryo in regard to cell polarity, migration, and the expression of key developmental genes;3) we will establish a direct ..
  62. Molecular Basis of Nuchal Edema
    Young Kwon Hong; Fiscal Year: 2013
    ..In this proposal, we provide our evidences that causatively link NE with dysregulated Notch signaling and propose to further dissect the molecular mechanism for NE. ..
    GERTRUD M SCHUPBACH; Fiscal Year: 2013
    ..Egfr by Grk initiates several different follicle cell responses and is required for axis formation of the egg and embryo. We have also shown that grk expression in oogenesis can be regulated by a checkpoint mechanism...
  64. Novel mechanisms of immune activation following allogeneic, hematopoietic stem ce
    JOHN JAMES LETTERIO; Fiscal Year: 2013
  65. The Molecular Genetics of Early Neurogenesis
    Ethan Bier; Fiscal Year: 2013
    ..e., BMPs repress CRM activity). We will follow a similar strategy to identify and characterize CRMs driving expression of laterally (Pax6) and ventrally (Nkx2.2.) genes. ..
  66. The role of the primitive choana in amniote craniofacial morphogenesis
    John Abramyan; Fiscal Year: 2013
    ..The presence of a new signalling center in the face will be investigated, and novel molecular causes for facial abnormalities such as cleft palate will be discovered. ..
  67. The amelioration of peroxisomal disorders due to defects in Pex10
    Lee A Niswander; Fiscal Year: 2013
    ..We will test the hypothesis that alterations in peroxisomal function in the embryo inhibit termination of axons at the synapse which disrupts the connectivity of the locomotor circuit...
  68. The S1P1 receptor regulates sarcomere assembly and cardiac development
    Roland S Wu; Fiscal Year: 2013
    ..The effects of this disruption will then be assayed by echocardiography, immunohistochemistry, and electron microscopy. ..
  69. Molecular mechanisms of electrical synapse formation in vivo
    Adam C Miller; Fiscal Year: 2013
    ..and their pre and postsynaptic partners, synapses, and function are exquisitely visualized in a living, vertebrate embryo. A forward genetic screen for mutations causing defects in the stereotyped M electrical synapses was performed ..
  70. The role of b-carotene in maternal-fetal nutrition
    Loredana Quadro; Fiscal Year: 2013
    ..Vitamin A is an essential nutrient for the developing embryo, which depends on maternal circulating vitamin A (or retinoids) to fulfill its needs...
  71. Understanding Wnt signaling through Ror and Ryk family receptor tyrosine kinases
    Mark A Lemmon; Fiscal Year: 2013
    ..In addition, our findings should open potential new avenues for therapeutic inhibition - as the roles of these Wnt-binding RTKs in disease become increasingly clear. ..
  72. Role of Cyclins in Brain Construction and Interneuron Genesis
    KRISTIN GIAMANCO; Fiscal Year: 2013
    ..These studies will contribute to the ultimate goal to devise more effective therapies for neuropsychiatric disorders. ..
  73. GATA1 Mutation in Defective Erythropoiesis
    John D Crispino; Fiscal Year: 2013
    ..The research described in this proposal will greatly expand our insights into how loss of the N-terminus of GATA1 alters erythropoiesis and will benefit patients with congenital anemia and DS-AMKL. ..
  74. Role of the Bradykinin Pathway in Craniofacial Development
    LAURA ANNE JACOX; Fiscal Year: 2013
    ..These studies interface with the NIDCR mission, and have potential to provide etiologies for craniofacial defects and suggest future early, minimally invasive treatments. ..
  75. Role of endocytosis by the neural crest in cardio-craniofacial development
    ANNA LUISE KEYTE; Fiscal Year: 2013
    Michael S Levine; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The early Drosophila embryo represents one of the premier systems for visualizing differential gene activity in animal development...
  77. Homolog orientation and segregation in acentrosomal meiosis
    Kim S McKim; Fiscal Year: 2013
    ..Defects in this process lead to aneuploidy in the fertilized egg and usually in death of the developing embryo. In humans, aneuploidy is a leading cause of spontaneous abortions and infertility in women and causes diseases ..
    Stuart H Orkin; Fiscal Year: 2013
    ..supported investigators through education and consultation, oocyte and blastocyst microinjections, assistance in embryo and hematological phenotyping, and de novo ES cell derivation...
  79. A Neural Development mRNA Decay Network
    Michael Cleary; Fiscal Year: 2013
    ..This work will generate a comprehensive and predictive network map of neural mRNA decay dynamics, thus filling a significant gap in current models of gene expression during neural development. ..
  80. Organization of the inactive X-chromosome
    Yuya Ogawa; Fiscal Year: 2013
    ..The proposed study in gene regulation during X-inactivation will potentially reveal a universal role for long non-protein coding (non-coding) RNAs in a wide variety of biological processes. ..
  81. Transcriptional regulation of neuronal differentiation
    Bennett G Novitch; Fiscal Year: 2013
  82. Oocyte polarity and mRNA localization in Zebrafish
    FLORENCE LOUISE MARLOW; Fiscal Year: 2013
    ..In model systems such as zebrafish where fertilization and development of the embryo occur externally every egg that is produced can be examined for developmental abnormalities...
  83. Regulation of Cytokinesis and Tumor Formation by RhoA
    ANN LOUISE MILLER; Fiscal Year: 2013
    ..Finally, I will test whether cytokinesis failure induced by other Rho zone regulators, especially those that are up- or down-regulated or mutated in human tumors, promotes tumor formation. ..
  84. Deleted in liver cancer 1 (DLC1) in liver development and disease
    Yi Ping Shih; Fiscal Year: 2013
    ..An understanding of the functional impact of DLC1 in liver development and disease progression will help find new early diagnosis or therapeutic targets for liver disease. ..
  85. Signaling pathways that mediate mammalian oocyte cortical mechanics
    Douglas N Robinson; Fiscal Year: 2013
    ..Defects in these processes during meiosis I or II can compromise egg quality and competence to form a healthy embryo, thus negatively impacting female fertility...
  86. Parental exposure to ionizing radiation and birth defects
    DOROTHY KIM WALLER; Fiscal Year: 2013
    ..to ionizing radiation in early pregnancy may result in the death of rapidly dividing cells in the developing embryo resulting in a birth defect...
  87. Proteolysis of cadherins in cranial neural crest
    ..cells that undergo an epithelial-to-mesenchymal transition (EMT) to acquire motility, migrate throughout the whole embryo and then differentiate into a diverse range of cell types including peripheral nervous system ganglia and neurons, ..
  88. Identification of XX DSD Mutations by RNA-seq and Comparative Genomics
    Vicki N Meyers-Wallen; Fiscal Year: 2013
    Joseph G Gall; Fiscal Year: 2013
    ..This stable intronic sequence (sis) RNA is transmitted to the embryo at fertilization and persists during early development until at least the blastula stage...
  90. The Tunicate Ciona: A New Model for the Effects of Aging on Tissue Regeneration
    William R Jeffery; Fiscal Year: 2013
    ..This study will develop the tunicate Ciona intestinalis, a vertebrate relative with powerful regeneration abilities that also recede with age, as a model to study the relationship between aging and tissue regeneration. ..
  91. Biophysical Mechanisms of Drosophila Development.
    Jennifer A Zallen; Fiscal Year: 2013
    ..In the Drosophila embryo, the polarized cell rearrangements that drive body axis elongation are guided by the spatial and temporal ..
  92. Functional and Phenotypic Characterization of a New FSGS Gene
    ..Our genetic and mechanistic approaches will advance our understanding of the molecular pathogenesis of podocyte phenotype changes in FSGS and lead to identification of novel therapeutic targets and less toxic pharmacologic approaches. ..
  93. Hybridization Chain Reaction: In Situ Amplification for Biological Imaging
    Niles A Pierce; Fiscal Year: 2013
    ..Our major goals are: Accurate and precise relative quantitation of mRNA abundance across whole-embryo images. Sub-cellular imaging of single mRNA transcripts with quantitative yield in whole-mount zebrafish embryos...
  94. Polarized transport in nervous system development and disease in zebrafish
    Philip Campbell; Fiscal Year: 2013
    ..The Zebrafish embryo is optically transparent and genetically accessible, allowing for in vivo imaging of disease models and ..
  95. Device for Hyperfast Scalable Vitrification of Germplasm in Large Volumes
    IGOR KATKOV; Fiscal Year: 2013
    ..In Phase II, we will build a closed modular stem for hyperfast cooling, cryogenic storage and shipment, and hyperfast thawing of cells and test Krioblast-3 on real germplasm cells. ..
    Christos Coutifaris; Fiscal Year: 2013
    ..It is anticipated that this trial will establish the standard of care for human embryo culture...
  97. Identifying transcription factors expressed by ventral spinal cord interneurons
    KATHARINE EMMA LEWIS; Fiscal Year: 2013
    ..of this research is to determine how different types of nerve cells (neurons) are made in a developing vertebrate embryo. This proposal specifically focuses on five distinct types of neurons that are located in the ventral spinal cord ..
  98. Ethanol-induced Lower Jaw Loss in Bmp Signaling Pathway Mutants
    CHARLES BENJAMIN LOVELY; Fiscal Year: 2013
    ..abstract_text> ..
  99. Does in utero exposure to domoic acid cause temporal lobe epilepsy?
    Paul S Buckmaster; Fiscal Year: 2013
    ..The investigators will determine whether or not exposure during pregnancy can cause temporal lobe epilepsy in laboratory mice and naturally exposed sea lions. ..
  100. Bio-imaging with Isothermal DNA Self-Assembly
    David Yu Zhang; Fiscal Year: 2013
    ..For in situ whole embryo mRNA imaging, geometrically precise DNA nanostructures will act as bright optical "tags" specific to each mRNA ..
  101. Uncovering mechanisms controlling notochord vacuole and spine morphogenesis
    Michel Bagnat; Fiscal Year: 2013
    ..notochord plays critical signaling roles during vertebrate development and acts as a hydrostatic skeleton for the embryo before bone formation...