poliovirus

Summary

Summary: A species of ENTEROVIRUS which is the causal agent of POLIOMYELITIS in humans. Three serotypes (strains) exist. Transmission is by the fecal-oral route, pharyngeal secretions, or mechanical vector (flies). Vaccines with both inactivated and live attenuated virus have proven effective in immunizing against the infection.

Top Publications

  1. pmc Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase
    Peng Gong
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523 1870, USA
    Proc Natl Acad Sci U S A 107:22505-10. 2010
  2. ncbi Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus
    B A Brown
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Virus Res 39:195-205. 1995
  3. pmc Poliovirus RNA is released from the capsid near a twofold symmetry axis
    Mihnea Bostina
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Virol 85:776-83. 2011
  4. pmc Complex dynamic development of poliovirus membranous replication complexes
    George A Belov
    Virginia Maryland College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742, USA
    J Virol 86:302-12. 2012
  5. pmc Catching a virus in the act of RNA release: a novel poliovirus uncoating intermediate characterized by cryo-electron microscopy
    Hazel C Levy
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA
    J Virol 84:4426-41. 2010
  6. pmc Genetic inactivation of poliovirus infectivity by increasing the frequencies of CpG and UpA dinucleotides within and across synonymous capsid region codons
    Cara C Burns
    Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 83:9957-69. 2009
  7. pmc An externalized polypeptide partitions between two distinct sites on genome-released poliovirus particles
    Jun Lin
    Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA
    J Virol 85:9974-83. 2011
  8. pmc Recombination between poliovirus and coxsackie A viruses of species C: a model of viral genetic plasticity and emergence
    Nicolas Combelas
    Biologie des Virus Enteriques, Institut Pasteur, Paris, France
    Viruses 3:1460-84. 2011
  9. ncbi Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily
    C L Mendelsohn
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032
    Cell 56:855-65. 1989
  10. pmc Cross talk between PML and p53 during poliovirus infection: implications for antiviral defense
    Mathieu Pampin
    CNRS FRE 2944, Institut Lwoff, Villejuif, France
    J Virol 80:8582-92. 2006

Research Grants

Detail Information

Publications292 found, 100 shown here

  1. pmc Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase
    Peng Gong
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523 1870, USA
    Proc Natl Acad Sci U S A 107:22505-10. 2010
    ..To further our understanding of RdRP function, we assembled, purified, and then crystallized poliovirus elongation complexes after multiple rounds of nucleotide incorporation...
  2. ncbi Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus
    B A Brown
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Virus Res 39:195-205. 1995
    Enterovirus 71 (EV71) is capable of causing paralytic disease indistinguishable from poliomyelitis due to poliovirus. To determine the relationship of EV71 to poliovirus and other enteroviruses, two strains of EV71 have been cloned and ..
  3. pmc Poliovirus RNA is released from the capsid near a twofold symmetry axis
    Mihnea Bostina
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Virol 85:776-83. 2011
    After recognizing and binding to its host cell, poliovirus (like other nonenveloped viruses) faces the challenge of translocating its genome across a cellular membrane and into the cytoplasm...
  4. pmc Complex dynamic development of poliovirus membranous replication complexes
    George A Belov
    Virginia Maryland College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742, USA
    J Virol 86:302-12. 2012
    ..Here we utilize electron tomography and other methods to investigate the remodeling of membranes in poliovirus-infected cells...
  5. pmc Catching a virus in the act of RNA release: a novel poliovirus uncoating intermediate characterized by cryo-electron microscopy
    Hazel C Levy
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA
    J Virol 84:4426-41. 2010
    b>Poliovirus infection requires that the particle undergo a series of conformational transitions that lead to cell entry and genome release...
  6. pmc Genetic inactivation of poliovirus infectivity by increasing the frequencies of CpG and UpA dinucleotides within and across synonymous capsid region codons
    Cara C Burns
    Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 83:9957-69. 2009
    Replicative fitness of poliovirus can be modulated systematically by replacement of preferred capsid region codons with synonymous unpreferred codons...
  7. pmc An externalized polypeptide partitions between two distinct sites on genome-released poliovirus particles
    Jun Lin
    Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA
    J Virol 85:9974-83. 2011
    During cell entry, native poliovirus (160S) converts to a cell-entry intermediate (135S) particle, resulting in the externalization of capsid proteins VP4 and the amino terminus of VP1 (residues 1 to 53)...
  8. pmc Recombination between poliovirus and coxsackie A viruses of species C: a model of viral genetic plasticity and emergence
    Nicolas Combelas
    Biologie des Virus Enteriques, Institut Pasteur, Paris, France
    Viruses 3:1460-84. 2011
    Genetic recombination in RNA viruses was discovered many years ago for poliovirus (PV), an enterovirus of the Picornaviridae family, and studied using PV or other picornaviruses as models...
  9. ncbi Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily
    C L Mendelsohn
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032
    Cell 56:855-65. 1989
    Restriction of poliovirus replication to a few sites in the infected primate host appears to be controlled by the expression of viral receptors...
  10. pmc Cross talk between PML and p53 during poliovirus infection: implications for antiviral defense
    Mathieu Pampin
    CNRS FRE 2944, Institut Lwoff, Villejuif, France
    J Virol 80:8582-92. 2006
    ..We describe herein the molecular events following poliovirus infection that lead to PML-dependent p53 activation and protection against virus infection...
  11. pmc Expression of foreign proteins by poliovirus polyprotein fusion: analysis of genetic stability reveals rapid deletions and formation of cardioviruslike open reading frames
    S Mueller
    Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, 11794, USA
    J Virol 72:20-31. 1998
    ..or the gag gene (encoding p17-p24) of human immunodeficiency virus type 1 (HIV-1) to the N terminus of the poliovirus polyprotein. All poliovirus expression vectors constructed by us and those obtained from Andino et al...
  12. ncbi Vaccine-derived polioviruses and the endgame strategy for global polio eradication
    Olen M Kew
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Annu Rev Microbiol 59:587-635. 2005
    As the global eradication of wild poliovirus nears, the World Health Organization (WHO) is addressing challenges unprecedented in public health...
  13. pmc Poliovirus cell entry: common structural themes in viral cell entry pathways
    James M Hogle
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Microbiol 56:677-702. 2002
    ..This model has striking parallels with emerging models for the maturation and cell entry of more complex enveloped viruses such as influenza virus and HIV...
  14. ncbi Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155)
    Anja Fuchs
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Immunol 172:3994-8. 2004
    The poliovirus receptor (PVR) belongs to a large family of Ig molecules called nectins and nectin-like proteins, which mediate cell-cell adhesion, cell migration, and serve as entry receptors for viruses...
  15. pmc Structural basis for proteolysis-dependent activation of the poliovirus RNA-dependent RNA polymerase
    Aaron A Thompson
    Program in Cellular and Molecular Biology, Colorado State University, Fort Collins 80523, USA
    EMBO J 23:3462-71. 2004
    The active RNA-dependent RNA polymerase of poliovirus, 3Dpol, is generated by cleavage of the 3CDpro precursor protein, a protease that has no polymerase activity despite containing the entire polymerase domain...
  16. pmc Specific cleavage of the nuclear pore complex protein Nup62 by a viral protease
    Nogi Park
    Department of Basic Medical Sciences, University of Arizona College of Medicine Phoenix, Phoenix, Arizona 85004, USA
    J Biol Chem 285:28796-805. 2010
    ..shown that several nucleoporins, including Nup62 are degraded in cells infected with human rhinovirus (HRV) and poliovirus (PV) and that this contributes to the disruption of certain nuclear transport pathways...
  17. ncbi Differential localization of neurons susceptible to enterovirus 71 and poliovirus type 1 in the central nervous system of cynomolgus monkeys after intravenous inoculation
    Noriyo Nagata
    Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan
    J Gen Virol 85:2981-9. 2004
    b>Poliovirus and enterovirus 71 (EV71) are both neurotropic enteroviruses that cause serious neurological diseases, such as poliomyelitis and encephalitis...
  18. ncbi Reaching the last one per cent: progress and challenges in global polio eradication
    Olen Kew
    Division of Viral Diseases, G 10, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Curr Opin Virol 2:188-98. 2012
    ..and Nigeria have been offset by continued endemicity in Pakistan and Afghanistan, and repeated reseeding of wild poliovirus into polio-free areas has led to large outbreaks and re-established transmission...
  19. pmc Bottleneck-mediated quasispecies restriction during spread of an RNA virus from inoculation site to brain
    Julie K Pfeiffer
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 103:5520-5. 2006
    The amplification of RNA viruses such as poliovirus is associated with high error rates, and the resulting diversity likely facilitates viral survival within an infected host...
  20. ncbi Mechanistic intersections between picornavirus translation and RNA replication
    Sarah Daijogo
    Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, California, USA
    Adv Virus Res 80:1-24. 2011
    ..The most extensively studied picornavirus, poliovirus, will be the focus of this review...
  21. pmc Evaluation of murine norovirus, feline calicivirus, poliovirus, and MS2 as surrogates for human norovirus in a model of viral persistence in surface water and groundwater
    Jinhee Bae
    Johns Hopkins Bloomberg School of Public Health, Department of Environmental Health Sciences, 615 N Wolfe St, Room E6620, Baltimore, MD 21205 2103, USA
    Appl Environ Microbiol 74:477-84. 2008
    ..The NoV surrogates murine NoV (MNV), feline calicivirus (FCV), poliovirus (PV), and male-specific coliphage MS2, in conjunction with Norwalk virus (NV), were spiked into surface water ..
  22. pmc Delayed kinetics of poliovirus RNA synthesis in a human cell line with reduced levels of hnRNP C proteins
    Jo Ellen Brunner
    Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA 92697, USA
    Virology 400:240-7. 2010
    ..Infection of HeLa cells with poliovirus causes hnRNP C to re-localize from the nucleus, where it is normally retained during interphase, to the ..
  23. ncbi Poliovirus serotype-specific VP1 sequencing primers
    David R Kilpatrick
    Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Virol Methods 174:128-30. 2011
    The Global Polio Laboratory Network routinely uses poliovirus-specific PCR primers and probes to determine the serotype and genotype of poliovirus isolates obtained as part of global poliovirus surveillance...
  24. ncbi Implications of a circulating vaccine-derived poliovirus in Nigeria
    Helen E Jenkins
    Medical Research Council Centre for Outbreak Analysis and Modelling, Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, United Kingdom
    N Engl J Med 362:2360-9. 2010
    The largest recorded outbreak of a circulating vaccine-derived poliovirus (cVDPV), detected in Nigeria, provides a unique opportunity to analyze the pathogenicity of the virus, the clinical severity of the disease, and the effectiveness ..
  25. pmc From emergence to eradication: the epidemiology of poliomyelitis deconstructed
    Neal Nathanson
    Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6021, USA
    Am J Epidemiol 172:1213-29. 2010
    ..Beginning in 1955, the creation of poliovirus vaccines led to a stepwise reduction in poliomyelitis, culminating in the unpredicted elimination of wild ..
  26. pmc Modification of cellular autophagy protein LC3 by poliovirus
    Matthew P Taylor
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Virol 81:12543-53. 2007
    b>Poliovirus infection remodels intracellular membranes, creating a large number of membranous vesicles on which viral RNA replication occurs...
  27. pmc A critical role of a cellular membrane traffic protein in poliovirus RNA replication
    George A Belov
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 4:e1000216. 2008
    Replication of many RNA viruses is accompanied by extensive remodeling of intracellular membranes. In poliovirus-infected cells, ER and Golgi stacks disappear, while new clusters of vesicle-like structures form sites for viral RNA ..
  28. ncbi Refined X-ray crystallographic structure of the poliovirus 3C gene product
    S C Mosimann
    Medical Research Council of Canada Group in Protein Structure and Function Department of Biochemistry, University of Alberta, Edmonton, AB, T6G 2H7, Canada
    J Mol Biol 273:1032-47. 1997
    The X-ray crystallographic structure of the recombinant poliovirus 3C gene product (Mahoney strain) has been determined by single isomorphous replacement and non-crystallographic symmetry averaging and refined at 2.1 A resolution...
  29. ncbi Outbreak of poliomyelitis in Hispaniola associated with circulating type 1 vaccine-derived poliovirus
    Olen Kew
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention CDC, 1600 Clifton Road, Atlanta, GA 30333, USA
    Science 296:356-9. 2002
    ..vaccinated children, and cases occurred in communities with very low (7 to 40%) rates of coverage with oral poliovirus vaccine (OPV)...
  30. pmc Stabilization of poliovirus polymerase by NTP binding and fingers-thumb interactions
    Aaron A Thompson
    Program in Cellular and Molecular Biology, Colorado State University, Fort Collins, CO 80523 1870, USA
    J Mol Biol 366:1459-74. 2007
    ..We have solved the crystal structures of poliovirus polymerase (3D(pol)) in complex with all four NTPs, showing that they all bind in a common pre-insertion site ..
  31. pmc Neurovirulent vaccine-derived polioviruses in sewage from highly immune populations
    Lester M Shulman
    Central Virology Laboratory, Public Health Services, Ministry of Health, Chaim Sheba Medical Center, Tel Hashomer, Israel
    PLoS ONE 1:e69. 2006
    ..6x10(6)) and Group 2 (2006, 2 systems, populations 0.7x10(6) and 5x10(4))...
  32. pmc A putative loop E motif and an H-H kissing loop interaction are conserved and functional features in a group C enterovirus RNA that inhibits ribonuclease L
    Hannah L Townsend
    Department of Microbiology, University of Colorado Denver, School of Medicine, Aurora, Colorado 80045, USA
    RNA Biol 5:263-72. 2008
    A phylogenetically conserved RNA structure within the open reading frame of poliovirus and other group C enteroviruses functions as a competitive inhibitor of the antiviral endoribonuclease RNase L...
  33. pmc Bidirectional increase in permeability of nuclear envelope upon poliovirus infection and accompanying alterations of nuclear pores
    George A Belov
    M P Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow
    J Virol 78:10166-77. 2004
    b>Poliovirus and some other picornaviruses trigger relocation of certain nuclear proteins into the cytoplasm...
  34. pmc A template RNA entry channel in the fingers domain of the poliovirus polymerase
    Matthew G Kortus
    Department of Biochemistry and Molecular Biology, Colorado State University, 1870 Campus Delivery, Colorado State University, Fort Collins, CO 80523 1870, USA
    J Mol Biol 417:263-78. 2012
    ..Structures of 3D(pol) from poliovirus, coxsackievirus, human rhinoviruses, and other picornaviruses reveal a putative template RNA entry channel on ..
  35. pmc A phylogenetically conserved RNA structure in the poliovirus open reading frame inhibits the antiviral endoribonuclease RNase L
    Jian Qiu Han
    Department of Microbiology, School of Medicine, University of Colorado, Mail Stop 8333, Room P18 9116, 12800 E 19th Ave, Aurora, CO 80045, USA
    J Virol 81:5561-72. 2007
    RNase L is an antiviral endoribonuclease that cleaves viral mRNAs after single-stranded UA and UU dinucleotides. Poliovirus (PV) mRNA is surprisingly resistant to cleavage by RNase L due to an RNA structure in the 3C(Pro) open reading ..
  36. pmc Analysis of poliovirus protein 3A interactions with viral and cellular proteins in infected cells
    Natalya L Teterina
    Laboratory of Infections Diseases, National Institutes of Health, Bldg 33, Room 3W10A 2, 33 North Drive, MSC 3203, Bethesda, MD 20892 3203, USA
    J Virol 85:4284-96. 2011
    b>Poliovirus proteins 3A and 3AB are small, membrane-binding proteins that play multiple roles in viral RNA replication complex formation and function...
  37. pmc Poliovirus-mediated disruption of cytoplasmic processing bodies
    Jonathan D Dougherty
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Virol 85:64-75. 2011
    ..b>Poliovirus (PV) is a plus-strand RNA virus containing a genome that is a functional mRNA; thus, we investigated if PV ..
  38. ncbi Immunogenicity of supplemental doses of poliovirus vaccine for children aged 6-9 months in Moradabad, India: a community-based, randomised controlled trial
    Concepcion F Estivariz
    Global Immunization Division, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Lancet Infect Dis 12:128-35. 2012
    The continued presence of polio in northern India poses challenges to the interruption of wild poliovirus transmission and the management of poliovirus risks in the post-eradication era...
  39. ncbi Poliomyelitis outbreaks in Angola genetically linked to India: risk factors and implications for prevention of outbreaks due to wild poliovirus importations
    Sarah Kidd
    Epidemic Intelligence Service, Centers for Disease Control and Prevention, Atlanta, GA United States
    Vaccine 29:3760-6. 2011
    We conducted an investigation of two outbreaks of poliomyelitis in Angola during 2007-2008 due to wild poliovirus (WPV) genetically linked to India...
  40. ncbi Mucosal immunity after vaccination with monovalent and trivalent oral poliovirus vaccine in India
    Nicholas C Grassly
    Department of Infectious Disease Epidemiology, Imperial College London, United Kingdom
    J Infect Dis 200:794-801. 2009
    Persistent wild-poliovirus transmission, particularly in India, has raised questions about the degree of mucosal immunity induced by oral poliovirus vaccine (OPV) in tropical countries.
  41. pmc The multifaceted poliovirus 2A protease: regulation of gene expression by picornavirus proteases
    Alfredo Castello
    European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    J Biomed Biotechnol 2011:369648. 2011
    ..This is the case of poliovirus, which abrogates cellular translation soon after virus internalization...
  42. pmc Dendritic cells and macrophages are productively infected by poliovirus
    Rahnuma Wahid
    Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Virol 79:401-9. 2005
    Expression of the poliovirus receptor (PVR) on cells is a major host determinant of infection by poliovirus...
  43. pmc Long-range interaction networks in the function and fidelity of poliovirus RNA-dependent RNA polymerase studied by nuclear magnetic resonance
    Xiaorong Yang
    Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA
    Biochemistry 49:9361-71. 2010
    The fidelity of the poliovirus RNA-dependent RNA polymerase (3D(pol)) plays a direct role in the genomic evolution and pathogenesis of the virus...
  44. pmc Genome delivery and ion channel properties are altered in VP4 mutants of poliovirus
    Pranav Danthi
    Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205, USA
    J Virol 77:5266-74. 2003
    During entry into host cells, poliovirus undergoes a receptor-mediated conformational transition to form 135S particles with irreversible exposure of VP4 capsid sequences and VP1 N termini...
  45. pmc Poliovirus RNA-dependent RNA polymerase (3Dpol): pre-steady-state kinetic analysis of ribonucleotide incorporation in the presence of Mg2+
    Jamie J Arnold
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 43:5126-37. 2004
    ..kinetic mechanism for correct nucleotide incorporation catalyzed by the RNA-dependent RNA polymerase from poliovirus, 3D(pol). The phosphoryl-transfer step is flanked by two isomerization steps...
  46. pmc Genetic determinants of cell type-specific poliovirus propagation in HEK 293 cells
    Stephanie A Campbell
    Dept of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3020, Durham, NC 27710, USA
    J Virol 79:6281-90. 2005
    The ability of poliovirus to propagate in neuronal cells can be reduced by introducing appropriate nucleotide substitutions into the viral genome...
  47. ncbi Asymptomatic wild-type poliovirus infection in India among children with previous oral poliovirus vaccination
    Nicholas C Grassly
    Department of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom
    J Infect Dis 201:1535-43. 2010
    Mucosal immunity induced by oral poliovirus vaccine (OPV) is imperfect and potentially allows immunized individuals to participate in asymptomatic wild-type poliovirus transmission in settings with efficient fecal-oral transmission of ..
  48. pmc Motif D of viral RNA-dependent RNA polymerases determines efficiency and fidelity of nucleotide addition
    Xiaorong Yang
    Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA
    Structure 20:1519-27. 2012
    ..We conclude that motif D of RdRps and, by inference, RTs is the functional equivalent to the fidelity helix of other polymerases...
  49. ncbi Effect of different vaccination schedules on excretion of oral poliovirus vaccine strains
    Majid Laassri
    Center for Biologics Evaluation and Research, Food and Drug Administration, Baltimore, Maryland, USA
    J Infect Dis 192:2092-8. 2005
    Inactivated poliovirus vaccine (IPV) is believed to induce significantly lower mucosal immunity than oral poliovirus vaccine (OPV). Most of the data supporting this were generated before enhanced IPV (eIPV) was introduced...
  50. pmc Cholesterol removal by methyl-beta-cyclodextrin inhibits poliovirus entry
    Pranav Danthi
    Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Virol 78:33-41. 2004
    Upon binding to the poliovirus receptor (PVR), the poliovirus 160S particles undergo a conformational transition to generate 135S particles, which are believed to be intermediates in the virus entry process...
  51. ncbi Inhibition of nucleo-cytoplasmic trafficking by RNA viruses: targeting the nuclear pore complex
    Kurt E Gustin
    Department of Microbiology, University of Idaho, Moscow, ID 83844, USA
    Virus Res 95:35-44. 2003
    ..This review will examine the recent evidence showing that poliovirus and vesicular stomatitis virus (VSV) can inhibit nucleo-cytoplasmic transport within cells...
  52. pmc Structure of the Fab-labeled "breathing" state of native poliovirus
    Jun Lin
    Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah, USA
    J Virol 86:5959-62. 2012
    At 37°C, the structure of poliovirus is dynamic, and internal polypeptides VP4 and N terminus of VP1 (residues 1 to 53) externalize reversibly...
  53. ncbi The poliovirus receptor CD155 mediates cell-to-matrix contacts by specifically binding to vitronectin
    R Lange
    Department of Tumor and Immunogenetics, Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, Berlin, 13092, Germany
    Virology 285:218-27. 2001
    The human receptor for poliovirus (CD155) is an immunoglobulin-like molecule with unknown normal function(s)...
  54. pmc A statistical model of the international spread of wild poliovirus in Africa used to predict and prevent outbreaks
    Kathleen M O'Reilly
    Medical Research Council Centre for Outbreak Analysis and Modelling, Department of Infectious Disease Epidemiology, School of Public Health, Imperial College London, London, United Kingdom
    PLoS Med 8:e1001109. 2011
    Outbreaks of poliomyelitis in African countries that were previously free of wild-type poliovirus cost the Global Polio Eradication Initiative US$850 million during 2003-2009, and have limited the ability of the program to focus on ..
  55. pmc RNA transfer from poliovirus 135S particles across membranes is mediated by long umbilical connectors
    Mike Strauss
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA
    J Virol 87:3903-14. 2013
    During infection, the binding of poliovirus to its cell surface receptor at 37°C triggers an expansion of the virus in which internal polypeptides that bind to membranes are externalized...
  56. ncbi Induction of mucosal immunity by inactivated poliovirus vaccine is dependent on previous mucosal contact with live virus
    T M Herremans
    Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment RIVM, Bilthoven, The Netherlands
    J Immunol 162:5011-8. 1999
    The inactivated poliovirus vaccine (IPV) is used for protection against poliomyelitis in The Netherlands. It is not clear, however, whether IPV vaccination can lead to priming of the mucosal immune system and the induction of IgA...
  57. ncbi Role of environmental poliovirus surveillance in global polio eradication and beyond
    T Hovi
    National Institute for Health and Welfare, Mannerheimintie 166, Helsinki, Finland
    Epidemiol Infect 140:1-13. 2012
    Environmental poliovirus surveillance (ENV) means monitoring of poliovirus (PV) transmission in human populations by examining environmental specimens supposedly contaminated by human faeces...
  58. pmc Dual stem loops within the poliovirus internal ribosomal entry site control neurovirulence
    M Gromeier
    Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
    J Virol 73:958-64. 1999
    In the human central nervous system, susceptibility to poliovirus (PV) infection is largely confined to a specific subpopulation of neuronal cells...
  59. pmc Translation without eIF2 promoted by poliovirus 2A protease
    Natalia Redondo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Madrid, Spain
    PLoS ONE 6:e25699. 2011
    b>Poliovirus RNA utilizes eIF2 for the initiation of translation in cell free systems. Remarkably, we now describe that poliovirus translation takes place at late times of infection when eIF2 is inactivated by phosphorylation...
  60. ncbi Polypyrimidine tract-binding proteins are cleaved by caspase-3 during apoptosis
    Sung Hoon Back
    National Research Laboratory, Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San31, Hyoja Dong, Pohang, Kyungbuk 790 784, Korea
    J Biol Chem 277:27200-9. 2002
    ..This suggests that translation of some IRES-containing mRNAs is regulated by proteolytic cleavage of PTB during apoptosis...
  61. pmc Poliovirus Sabin type 1 neutralization epitopes recognized by immunoglobulin A monoclonal antibodies
    L Fiore
    Laboratorio di Virologia, Istituto Superiore di Sanita, Rome, Italy
    J Virol 71:6905-12. 1997
    ..on humoral neutralizing antibodies, both after natural (wild virus or vaccine) infection and after inactivated poliovirus vaccine inoculation...
  62. pmc Early events in poliovirus infection: virus-receptor interactions
    V R Racaniello
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 93:11378-81. 1996
    The interaction of poliovirus with its cell receptor initiates conformational changes that lead to uncoating of the viral RNA...
  63. ncbi Trial of a supplemental dose of four poliovirus vaccines
    R W Sutter
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    N Engl J Med 343:767-73. 2000
    The immunogenicity of oral poliovirus vaccine (OPV), particularly the type 3 component, is lower in infants in most developing countries than in infants in industrialized countries...
  64. ncbi Immunogenicity of bivalent types 1 and 3 oral poliovirus vaccine: a randomised, double-blind, controlled trial
    Roland W Sutter
    World Health Organization, Geneva, Switzerland
    Lancet 376:1682-8. 2010
    b>Poliovirus types 1 and 3 co-circulate in poliomyelitis-endemic countries. We aimed to assess the immunogenicity of a novel bivalent types 1 and 3 oral poliovirus vaccine (bOPV).
  65. ncbi Serological response and poliovirus excretion following different combined oral and inactivated poliovirus vaccines immunization schedules
    Isabelle Parent Du Chatelet
    Association pour l Aide a la Medecine Preventive, 25 rue du Dr Roux, 75724 Cedex 15, Paris, France
    Vaccine 21:1710-8. 2003
    ..Pakistan to compare humoral and mucosal immune responses against polioviruses in infants who received oral poliovirus vaccine (OPV) at birth and at 6, 10, and 14 weeks according to the Expanded Program on Immunization (EPI) with ..
  66. pmc Identification of tolerated insertion sites in poliovirus non-structural proteins
    Natalya L Teterina
    Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA
    Virology 409:1-11. 2011
    ..To identify regions in the poliovirus polyprotein that could potentially accommodate insertion of tags, transposon-mediated insertion mutagenesis was ..
  67. pmc Identification of two functionally redundant RNA elements in the coding sequence of poliovirus using computer-generated design
    Yutong Song
    Department of Molecular Genetics and Microbiology and Computer Science, Stony Brook University, Stony Brook, NY 11794, USA
    Proc Natl Acad Sci U S A 109:14301-7. 2012
    ..We use unique approaches to identify functional RNA elements in the coding sequence of poliovirus (PV), a plus strand RNA virus...
  68. pmc The 5'CL-PCBP RNP complex, 3' poly(A) tail and 2A(pro) are required for optimal translation of poliovirus RNA
    Sushma A Ogram
    Department of Biochemistry and Molecular Biology, University of Florida, College of Medicine, Gainesville, FL 32610 0245, USA
    Virology 397:14-22. 2010
    ..These results are consistent with a model in which the 5'CL-PCBP complex interacts with the 3' poly(A)-PABP complex to form a 5'-3' circular complex that facilitates ribosome reloading and stimulates PV RNA translation...
  69. pmc Systematic review of mucosal immunity induced by oral and inactivated poliovirus vaccines against virus shedding following oral poliovirus challenge
    Thomas R Hird
    Department of Infectious Disease Epidemiology, Imperial College London, Norfolk Place, London, United Kingdom
    PLoS Pathog 8:e1002599. 2012
    Inactivated poliovirus vaccine (IPV) may be used in mass vaccination campaigns during the final stages of polio eradication...
  70. ncbi Preexisting poliovirus-specific IgA in the circulation correlates with protection against virus excretion in the elderly
    Anne Marie Buisman
    Center for Infectious Diseases Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
    J Infect Dis 197:698-706. 2008
    Epidemiological studies have indicated that at least 10% of the Dutch elderly do not have poliovirus serotype-specific neutralizing antibody titers and might be at risk for poliovirus infection...
  71. ncbi A "slide-back" mechanism for the initiation of protein-primed RNA synthesis by the RNA polymerase of poliovirus
    Aniko V Paul
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, New York 11790, USA
    J Biol Chem 278:43951-60. 2003
    b>Poliovirus RNA replication is initiated when a molecule of UMP is covalently linked to the hydroxyl group of a tyrosine in the terminal protein VPg...
  72. ncbi Temperature-sensitive mutants of enterovirus 71 show attenuation in cynomolgus monkeys
    Minetaro Arita
    Department of Virology II, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    J Gen Virol 86:1391-401. 2005
    ..EV71 prototype BrCr was constructed; temperature-sensitive (ts) mutations of an attenuated strain of EV71 or of poliovirus (PV) Sabin vaccine strains were then introduced into the infectious clone...
  73. ncbi Vaccine-derived poliovirus (VDPV): Impact on poliomyelitis eradication
    Philip Minor
    Division of Virology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshirre EN6 3QG, UK
    Vaccine 27:2649-52. 2009
    The live attenuated strains used in the oral poliovirus (OPV) have been the main tool in the WHO polio eradication programme. However, these strains replicate in the human gut and are excreted for several weeks after immunisation...
  74. pmc The linker domain of poly(rC) binding protein 2 is a major determinant in poliovirus cap-independent translation
    Polen Sean
    Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA 92697, USA
    Virology 378:243-53. 2008
    b>Poliovirus, a member of the enterovirus genus in the family Picornaviridae, is the causative agent of poliomyelitis...
  75. pmc Environmental poliovirus surveillance during oral poliovirus vaccine and inactivated poliovirus vaccine use in Córdoba Province, Argentina
    Judith E Mueller
    Agence de Medecine Preventive, Paris, France
    Appl Environ Microbiol 75:1395-401. 2009
    This study compares the presence of environmental poliovirus in two Argentinean populations using oral poliovirus vaccine (OPV) or inactivated poliovirus vaccine (IPV)...
  76. ncbi Recurrent isolation of poliovirus 3 strains with chimeric capsid protein Vp1 suggests a recombination hot-spot site in Vp1
    Soile Blomqvist
    National Institute for Health and Welfare THL, Department of Infectious Disease Surveillance and Control, Intestinal Viruses Unit, P O Box 30, 00271 Helsinki, Finland
    Virus Res 151:246-51. 2010
    Five oral poliovirus vaccine (OPV) strains carrying an intertypic PV3/PV2 recombination in VP1 capsid protein were isolated during poliovirus surveillance...
  77. pmc Direct interaction between two viral proteins, the nonstructural protein 2C and the capsid protein VP3, is required for enterovirus morphogenesis
    Ying Liu
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, New York, United States of America
    PLoS Pathog 6:e1001066. 2010
    ..of morphogenesis of plus-stranded RNA viruses belonging to the genus Enterovirus of Picornaviridae, including poliovirus (PV), is not understood. Numerous attempts to identify an RNA encapsidation signal have failed...
  78. pmc Molecular evolution of a type 1 wild-vaccine poliovirus recombinant during widespread circulation in China
    H M Liu
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 74:11153-61. 2000
    ..All recombinants had a 367-nt block of sequence (nt 3271 to 3637) derived from the Sabin 1 oral poliovirus vaccine strain spanning the 3'-terminal sequences of VP1 (115 nt) and the 5' half of 2A (252 nt)...
  79. pmc In vitro antiviral activity of V-073 against polioviruses
    M Steven Oberste
    Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Antimicrob Agents Chemother 53:4501-3. 2009
    ..003 to 0.126 microM. Ninety percent of the polioviruses tested were inhibited at EC(50)s of < or = 0.076 microM (MIC90 = 32 ng/ml). V-073 is a promising antiviral candidate for the posteradication management of poliovirus incidents.
  80. ncbi Anti-poliovirus activity of Baccharis dracunculifolia and propolis by cell viability determination and real-time PCR
    M C Búfalo
    Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil
    J Appl Microbiol 107:1669-80. 2009
    ..of this work was to evaluate the antiviral activities of Baccharis dracunculifolia (extract and essential oil), propolis and some isolated compounds (caffeic and cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells.
  81. pmc Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site
    Hidemi Toyoda
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11790, USA
    J Virol 81:10017-28. 2007
    The 5' nontranslated region of poliovirus RNA contains two highly structured regions, the cloverleaf (CL) and the internal ribosomal entry site (IRES)...
  82. ncbi Cleavage of eIF4G by HIV-1 protease: effects on translation
    Celia Perales
    Centro de Biologi a Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autonoma CSIC, Cantoblanco 28049, Madrid, Spain
    FEBS Lett 533:89-94. 2003
    ..specific proteolytic activity of the retroviral protease on eIF4GI was more selective than that observed with poliovirus 2A(pro)...
  83. pmc Different effect of proteasome inhibition on vesicular stomatitis virus and poliovirus replication
    Nickolay Neznanov
    Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, United States of America
    PLoS ONE 3:e1887. 2008
    ..we examined the effect of proteasome inhibitors on the replication of vesicular stomatitis virus (VSV) and poliovirus. We found that the proteasome inhibitors significantly suppressed VSV protein synthesis, virus accumulation, and ..
  84. pmc Calibration of multiple poliovirus molecular clocks covering an extended evolutionary range
    Jaume Jorba
    Polio and Picornavirus Laboratory Branch, G 10, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Virol 82:4429-40. 2008
    We have calibrated five different molecular clocks for circulating poliovirus based upon the rates of fixation of total substitutions (K(t)), synonymous substitutions (K(s)), synonymous transitions (A(s)), synonymous transversions (B(s)), ..
  85. pmc Effects of poliovirus infection on nucleo-cytoplasmic trafficking and nuclear pore complex composition
    K E Gustin
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    EMBO J 20:240-9. 2001
    ..We show here that infection of cells with poliovirus results in the cytoplasmic accumulation of a variety of shuttling and non-shuttling nuclear proteins that use ..
  86. pmc Cooperative effect of the attenuation determinants derived from poliovirus sabin 1 strain is essential for attenuation of enterovirus 71 in the NOD/SCID mouse infection model
    Minetaro Arita
    Department of Virology II, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    J Virol 82:1787-97. 2008
    ..the cynomolgus monkey infection model; this strain contains the attenuation determinants derived from the type 1 poliovirus vaccine strain, Sabin 1 [PV1(Sabin)], in the 5' nontranslated region (NTR), 3D polymerase, and 3' NTR...
  87. pmc Prolonged replication of a type 1 vaccine-derived poliovirus in an immunodeficient patient
    O M Kew
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, National Immunization Program, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 36:2893-9. 1998
    VP1 sequences were determined for poliovirus type 1 isolates obtained over a 189-day period from a poliomyelitis patient with common variable immunodeficiency syndrome (a defect in antibody formation)...
  88. ncbi Oncolytic treatment and cure of neuroblastoma by a novel attenuated poliovirus in a novel poliovirus-susceptible animal model
    Hidemi Toyoda
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, New York 11794 5222, USA
    Cancer Res 67:2857-64. 2007
    ..b>Poliovirus targets motor neurons leading to irreversible paralysis...
  89. ncbi Effectiveness of immunization against paralytic poliomyelitis in Nigeria
    Helen E Jenkins
    Medical Research Council Centre for Outbreak Analysis and Modeling, Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, United Kingdom
    N Engl J Med 359:1666-74. 2008
    ..of paralytic poliomyelitis has declined in Nigeria since the introduction of newly licensed monovalent oral poliovirus vaccines and new techniques of vaccine delivery...
  90. ncbi Characterization of a rare natural intertypic type 2/type 3 penta-recombinant vaccine-derived poliovirus isolated from a child with acute flaccid paralysis
    Yong Zhang
    WHO WPRO Regional Polio Reference Laboratory and State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No 27 Nanwei Road, Xuanwu District, Beijing 100050, PR China
    J Gen Virol 91:421-9. 2010
    A type 2 vaccine-derived poliovirus (VDPV) (strain CHN1025), with a 1.1 % (10/903) difference from Sabin strain in the VP1 coding region, was isolated from a child with poliomyelitis caused by a poliovirus variant infection...
  91. ncbi Poliovirus, pathogenesis of poliomyelitis, and apoptosis
    B Blondel
    Laboratoire des Virus Entérotropes et Stratégies Antivirales, Institut Pasteur, 75724 Paris Cedex 15, France
    Curr Top Microbiol Immunol 289:25-56. 2005
    b>Poliovirus (PV) is the causal agent of paralytic poliomyelitis, an acute disease of the central nervous system (CNS) resulting in flaccid paralysis...
  92. ncbi Function of minor polypeptides in foot-and-mouth disease virus and poliovirus
    J F Newman
    US Dept of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944
    Trends Microbiol 2:494-7. 1994
    Foot-and-mouth disease virus and poliovirus each contain several minor polypeptides, in addition to the four structural proteins...
  93. ncbi In vitro antiviral activity of single domain antibody fragments against poliovirus
    Bert Thys
    Department of Pharmaceutical Biotechnology and Molecular Biology, Centre for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Antiviral Res 87:257-64. 2010
    ..good immune responses in camelids; therefore we hypothesized that VHHs selected from a dromedary immunized with poliovirus type 1 might inhibit the in vitro replication of poliovirus through binding to essential biological sites on the ..
  94. ncbi Two types of death of poliovirus-infected cells: caspase involvement in the apoptosis but not cytopathic effect
    V I Agol
    M P Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow, Region 142782, Russia
    Virology 252:343-53. 1998
    The death of poliovirus-infected cells may occur in two forms: canonical cytopathic effect (CPE) (on productive infections) or apoptosis (when the viral reproduction is hindered by certain drugs or some other restrictive conditions)...
  95. pmc Preparing an oncolytic poliovirus recombinant for clinical application against glioblastoma multiforme
    Christian Goetz
    Division of Neurosurgery, Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Cytokine Growth Factor Rev 21:197-203. 2010
    PVS-RIPO is a genetically recombinant, non-pathogenic poliovirus chimera with a tumor-specific conditional replication phenotype...
  96. pmc Differential rescue of poliovirus RNA replication functions by genetically modified RNA polymerase precursors
    Christopher T Cornell
    Department of Microbiology and Molecular Genetics, Med Sci B240, University of California, Irvine, CA 92697 4025, USA
    J Virol 78:13007-18. 2004
    We have previously described the RNA replication properties of poliovirus transcripts harboring chimeric RNA polymerase sequences representing suballelic exchanges between poliovirus type 1 (PV1) and coxsackievirus B3 (CVB3) utilizing an ..
  97. pmc An RNA element at the 5'-end of the poliovirus genome functions as a general promoter for RNA synthesis
    Dorothee A Vogt
    Department of Microbiology and Immunology, University of California, San Francisco, California, United States of America
    PLoS Pathog 6:e1000936. 2010
    ..An RNA structure formed around the 5'- end of the poliovirus genomic RNA has been implicated in the initiation of both negative- and positive-strand RNA synthesis...
  98. pmc Outbreak of type 2 vaccine-derived poliovirus in Nigeria: emergence and widespread circulation in an underimmunized population
    Steven Wassilak
    Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Infect Dis 203:898-909. 2011
    Wild poliovirus has remained endemic in northern Nigeria because of low coverage achieved in the routine immunization program and in supplementary immunization activities (SIAs)...
  99. pmc Strand-specific RNA synthesis determinants in the RNA-dependent RNA polymerase of poliovirus
    Christopher T Cornell
    Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine, California 92697 4025, USA
    J Virol 78:4397-407. 2004
    The viral RNA-dependent RNA polymerase (3D(pol)) is highly conserved between the closely related enteroviruses poliovirus type 1 (PV1) and coxsackievirus B3 (CVB3)...
  100. ncbi Correlation between recombination junctions and RNA secondary structure elements in poliovirus Sabin strains
    Evaggelos Dedepsidis
    Department of Biochemistry and Biotechnology, Microbiology Virology Laboratory, University of Thessaly, 26 Ploutonos and Aiolou Str, Larisa, Greece
    Virus Genes 41:181-91. 2010
    ..elements promote the distribution of certain types of recombination junctions in each one of the 2C and 3D poliovirus genomic regions (Sabin 3/Sabin 2 or Sabin 1 in 2C and Sabin 2/Sabin 1 or Sabin 3 in 3D), we searched in 2C and ..
  101. ncbi A global call for new polio vaccines
    David L Heymann
    Global Polio Eradication Initiative, World Health Organization, 20 Avenue Appia, CH 1211 Geneva, Switzerland
    Nature 434:699-700. 2005

Research Grants63

  1. Enhanced Membrane Systems for Supplying Quality Drinking Water
    Andrew Feiring; Fiscal Year: 2013
    ..g. E. Coli, Enterococcus Faecalis, MS2 bacteriophage Poliovirus, Cryptosporidium parvum, Legionelle pneumophila, and Salmonella enterica plus specific chemicals, phenol, and ..
  2. Enterovirus RNA Translation and Replication
    David J Barton; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Poliovirus and related human enteroviruses cause a diverse spectrum of human diseases including acute flaccid paralysis (paralytic poliomyelitis), aseptic meningitis, myocarditis, conjunctivitis and ..
  3. Molecular Biology of Eukaryotic Viruses
    Bert L Semler; Fiscal Year: 2012
    ..viruses/viral systems being studied include murine leukemia virus, a sheep retrovirus (JSRV), HIV, yeast Ty3, poliovirus, human rhinovirus, coxsackievirus, mouse hepatitis virus, SARS coronavirus, arenaviruses, herpes simplex virus, ..
  4. IRES-mediated translation initiation on viral mRNAs
    CHRISTOPHER ULRICH TRISTRAM HELLEN; Fiscal Year: 2013
    ..The mechanism of initiation on type 1 picornaviruses (e.g. poliovirus) is not known, but also involves specific interaction with eIF4G...
  5. ASM Conference on Viral Genome Replication
    Sean P J Whelan; Fiscal Year: 2011
    ..shortly be the second infection eradicated by vaccination and this will hopefully be followed by measles and poliovirus. Despite this, there are numerous chronic viral infections - HIV, HCV and HSV as well as acute viral infections -..
  6. Functions of 5'NCRs of picornavirus and cellular mRNAs
    Bert L Semler; Fiscal Year: 2013
    ..b>Poliovirus, coxsackievirus, and human rhinovirus are members of the Picornaviridae that share a common RNA secondary ..
  7. Reconstitution of Poliovirus and HCV RNA Replication Complexes on Membranes
    JENNIFER BARBARA PTACEK; Fiscal Year: 2010
    ..by applicant): It has been well established that all positive-strand RNA viruses of eukaryotic cells, including poliovirus and hepatitis C virus (HCV), replicate their genomes on the cytosolic surface of membranous structures...
  8. Antagonism of Innate Immunity By Picornaviruses
    Vincent R Racaniello; Fiscal Year: 2013
    ..Experiments in this application utilize a well-characterized human pathogen, poliovirus, to identify inhibitory ISG products and study their mechanism of action...
  9. The roles of acidic autophagosomes in production of infectious poliovirus
    William T Jackson; Fiscal Year: 2013
    ..In poliovirus (PV), a model for a host of medically important positive strand RNA viruses, two distinct classes of vesicle ..
  10. Electron microscopy studies of novel poliovirus genome-releasing intermediates
    Hazel C Levy; Fiscal Year: 2010
    ..These irreversible changes include externalization of peptides from the interior of the capsid shell. Poliovirus is the prototypical picornavirus and serves as an excellent model system...
  11. Genetic dissection of the RNAi-mediated antiviral immunity in C. elegans
    Shou Wei Ding; Fiscal Year: 2013
    ..Many RNA viruses such as influenza, hepatitis C virus, poliovirus, dengue virus and West Nile virus are important human pathogens...
  12. Oral Polio Vaccine Circulation in Mexico
    Stephanie B Troy; Fiscal Year: 2013
    ..The safest strategy after wild poliovirus eradication would be to globally stop using all OPV and use inactivated polio vaccine (IPV) until all OPV is out ..
  13. Validation of Src kinases as Anti-Dengue Viral Targets
    PRISCILLA LI NING YANG; Fiscal Year: 2012
    ..active against dengue viral isolates representative of each of the four dengue serotypes but not against poliovirus, an unrelated RNA virus. RNAi "knockdown" of c-Src phenocopies the inhibitory effect of the SFK inhibitors...
  14. Interdisciplinary Training in Virology and Gene Therapy
    Ren Sun; Fiscal Year: 2013
    ..With strong basic research programs on retroviruses, adenoviruses, SV40, hepatitis C, poliovirus, influenza virus and herpesviruses, our faculty has been and will be continuously developing viral vectors from ..
  15. Poliovirus Cell Entry Pathways
    James M Hogle; Fiscal Year: 2012
    ..This application proposes a program of structural studies of poliovirus cell entry intermediates as a simple model for understanding how nonenveloped viruses gain entry into the cell...
  16. Computational Studies of Early Stage Cell Entry Events by Non-enveloped Viruses
    ERIC ROBERT MAY; Fiscal Year: 2013
    ..I have chosen to study a model system for both of these phenomena. Poliovirus is structurally well characterized including a structure in complex with the poliovirus receptor...
  17. Enterovirus RNA Translation and Replication
    David Barton; Fiscal Year: 2009
    b>Poliovirus and related human enteroviruses cause a diverse spectrum of human diseases including acute flaccid paralysis (paralytic poliomyelitis), aseptic meningitis, myocarditis, conjunctivitis and fatal systemic infections of neonates...
  18. Towards a Vaccine for the Common Cold
    THOMAS JAMES SMITH; Fiscal Year: 2010
    ..are normally developed by either creating attenuated viral strains for inoculation, such as the Sabine strain of poliovirus, or by injecting the patients with purified viral proteins such as employed in the hepatitis B vaccine...
  19. POLIOVIRUS GENE FUNCTION AND REGULATION
    Bert L Semler; Fiscal Year: 2013
    ..b>Poliovirus, coxsackievirus, and human rhinovirus are members of the Picornaviridae that share a common RNA replication ..
  20. NIH Director's Pioneer Award
    Karla Kirkegaard; Fiscal Year: 2010
    ..Recently, we have consolidated this research to show that, for poliovirus, choices of drug target can be made so that drug-sensitive genomes dominantly inhibit the outgrowth of drug-..
  21. Subversion of Autophagy Pathway and Constituents by RNA viruses
    Karla Kirkegaard; Fiscal Year: 2013
    ..Several of these viruses, including poliovirus and Dengue virus, mimic or subvert the cellular pathway of autophagy to form double-membraned intracellular ..
  22. Assembly of Picornaviral Replication Complexes
    Olve Peersen; Fiscal Year: 2009
    ..B3 virus, rhinoviruses that cause more than half the occurrences of the common cold, and the paralyzing poliovirus. These viruses share a common life cycle where their RNA replication and viral assembly occurs in large membrane ..
  23. Towards the Disruption of Viral RNA Replication
    Esther Bullitt; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Diseases caused by positive-sense single-stranded RNA viruses, including poliovirus, rhinovirus, hepatitis C virus, and SARS, are critical health issues worldwide...
  24. PICORNAVIRUS MOLECULAR BIOLOGY
    Eckard Wimmer; Fiscal Year: 2013
    ..objective of the work proposed in this grant application to enhance our understanding of the mechanisms by which poliovirus (PV), an enterovirus belonging to the Picornaviridae, encapsidates its genome...
  25. Assembly of Picornaviral Replication Complexes
    OLVE BREIEN PEERSEN; Fiscal Year: 2013
    ..B3 virus, rhinoviruses that cause more than half the occurrences of the common cold, and the paralyzing poliovirus. These viruses share a life cycle where RNA replication and viral assembly occurs in large membrane anchored ..
  26. Picornavirus Genome Replication
    Craig E Cameron; Fiscal Year: 2013
    ..b>Poliovirus has served as an important model system for elucidation of viral factors, host factors and corresponding ..
  27. Oncolytic Virotherapy of Meningeal Cancer
    Matthias Gromeier; Fiscal Year: 2013
    ..We uncovered that oncogenic signaling controls anti-cancer activity of the prototype oncolytic poliovirus PVS-RIPO by modulating eIF4E activity...
  28. Host factors involved in viral RNA recombination
    Peter Nagy; Fiscal Year: 2006
    ..recombination has been demonstrated to occur for a large number of human, animal and plant viruses, including poliovirus, dengue virus, infectious bronchitis virus, influenza A virus, hantavirus, hepatitis D virus, Human Respiratory ..
  29. Nanocarriers for Vaccine Delivery
    John D Clements; Fiscal Year: 2013
    ..has been made towards the eradication of polio through the use of two different vaccines: the inactivated poliovirus vaccine (IPV) and the attenuated oral poliovirus vaccine (OPV)...
  30. Effect of tissue tropism on RNA viral evolutionary rates: three family-level anal
    SIOBAIN MARIE DEIRDRE DUFFY; Fiscal Year: 2012
    ..which include the leading cause of foodborne illness in this country, Picornaviridae, which include poliovirus, and Reoviridae, which include rotavirus, the most common cause of infant diarrhea worldwide...
  31. Assembly of a Dodecahedral Virus
    Adam Zlotnick; Fiscal Year: 2013
    ..We have chosen to examine Bovine Enterovirus (BEV) a non-pathogenic relation of Poliovirus and Human Enterovirus-71. BEV is non-pathogenic even in cattle, where it is endemic...
  32. Virology of the Central Nervous System
    John H Elder; Fiscal Year: 2010
    ..Appointments will be for a minimum of two years, with three years the norm. ..
  33. Study of a novel picornavirus genus associated with Acute Flaccid Paralysis
    Amit Kapoor; Fiscal Year: 2012
    ..In 2008 alone, 85,400 cases of acute flaccid paralysis (AFP) were reported from the countries with active poliovirus surveillance programs, of which less than 1,731 (2.03%) were found to be associated with poliovirus infection...
  34. Translation Regulation by Enterovirus Proteinase
    Richard E Lloyd; Fiscal Year: 2013
    ..by applicant): The long term goal of this research is to understand the mechanism by which enteroviruses such as poliovirus (PV) and Coxsackievirus (CVB3) control cellular and viral translation in infected cells and in turn, discern how ..
  35. Population Dynamics an Evolutionary Capacity of Viral Quasispecies
    ADAM SCOTT LAURING; Fiscal Year: 2012
    ..Aim 1) To characterize, at a functional level, the dynamics of an evolving viral population in vivo using poliovirus, an archetypal RNA virus...
  36. Mechanism of poliovirus replication
    Raul Andino; Fiscal Year: 2013
    ..The central hypothesis in this application is that RNA recombination plays a critical role in the generation of virus diversity and evolution and is critical for viral fitness and pathogenesis. ..
  37. Immunobiology and Prevention of H. pylori Disease
    Phillip Smith; Fiscal Year: 2009
    ..3) Delivery of H. pylori urease and either IL-10 or FoxP3 in a novel poliovirus vaccine vector to young mice protects the animals against H. pylori...
  38. Positive Strand RNA Viruses
    David L Woodland; Fiscal Year: 2013
    ..number of important human pathogens like hepatitis C virus, West Nile and dengue viruses, hepatitis A virus and poliovirus. Furthermore, the enormous variety of positive-stranded RNA viruses found in animals, and their evolutionary ..
  39. Targeting Translation Control in Malignant Glioma
    Matthias Gromeier; Fiscal Year: 2013
    ..We are exploiting this principle to target glioblastoma (GBM) with genetically recombinant poliovirus. Conventional protein synthesis is initiated upon engaging a complex protein network at the canonical 5'cap-..
  40. Manipulation of cellular translation machinery by viral RNAs
    Jeffrey S Kieft; Fiscal Year: 2013
    ..IRESs are central players in the infection cycles of many important viruses (e.g. hepatitis C, hepatitis A, poliovirus, HIV-1) and are significant regulators of gene expression...
  41. Enterovirus entry into polarized endothelial cells
    Carolyn B Coyne; Fiscal Year: 2012
    ..Our previous studies have established that coxsackievirus B (CVB) and poliovirus (PV) enter polarized cells by endocytic mechanisms that require activation of specific intracellular signaling ..
  42. Enteric virus-microbiota interactions
    Julie K Pfeiffer; Fiscal Year: 2013
    ..models, it was recently shown that intestinal bacteria promote infection with three unrelated enteric viruses, poliovirus, reovirus, and mouse mammary tumor virus...
  43. MOLECULAR BIOLOGY OF POLIOVIRUS RNA REPLICATION
    James Bert Flanegan; Fiscal Year: 2010
    b>Poliovirus is a member of the Picornaviridae family of small positive-strand RNA viruses. Diseases associated with these viruses include paralytic poliomyelitis, meningitis, encephalitis, myocarditis, hepatitis and the common cold...
  44. RNA-dependent RNA Polymerase
    Craig E Cameron; Fiscal Year: 2013
    ..Our program has employed a prototypical RNA virus, poliovirus (PV), and its RdRp (3Dpol) as our model system...
  45. CrPV IRES function and animal virus replication in yeast
    SUNNIE THOMPSON; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): Although poliovirus, the most well known enterovirus in the picornavirus family, is soon to be declared eradicated by the World Health Organization, there are 66 other distinct human ..
  46. GENETIC ANALYSIS OF PICORNAVIRUS RNA REPLICATION IN VIVO
    JAMES FLANEGAN; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): Poliovirus has served as the prototype virus for studying the replication mechanisms of picornaviruses and other (+) strand RNA viruses...
  47. STRUCTURE, SYNTHESIS AND ASSEMBLY OF PICORNAVIRUSES
    ROLAND RUECKERT; Fiscal Year: 1991
    ..the atomic structure of the protein subunit is now known for three of them: human rhinovirus 14 (HRV14), type 1 poliovirus (PV1) and mengovirus...
  48. STRUCTURE OF POLIOVIRUS/ANTIVIRAL DRUG COMPLEXES
    JAMES HOGLE; Fiscal Year: 1991
    ..We propose a series of crystallographic studies of complexes of several antiviral drugs with poliovirus. The goals of these studies will be twofold: 1) to provide a basis for the rational design of new antivirals ..
  49. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 2000
    ..To this end, we have developed replication-competent poliovirus recombinants that can carry and express antigens derived from HIV and the simian immunodeficiency virus (SIV)...
  50. Enterovirus RNA Translation and Replication
    David Barton; Fiscal Year: 2003
    ..b>Poliovirus, the prototypic enterovirus, is well characterized at the molecular level and still serves as the most ..
  51. Structual Analysis of Enterovirus Cell Entry Pathways
    JAMES HOGLE; Fiscal Year: 2002
    Over the past 10 years poliovirus and its close relatives (Coxsackieviruses, echoviruses and rhinoviruses) have emerged as model systems for probing the as yet poorly understood cell-entry mechanisms of non-enveloped viruses...
  52. STRUCTURE AND FUNCTION OF THE POLIOVIRUS RNA GENOME
    Peter Sarnow; Fiscal Year: 1991
    An ideal tool to study biosynthesis pathways in mammalian cells is a virus which interferes with these processes. Poliovirus is a positive-strand cytoplasmic RNA virus that undergoes extensive interactions with its host cell, including ..
  53. Cell Proteins Required for Function of the IRES
    VINCENT RACANIELLO; Fiscal Year: 2001
    ..cell proteins required for function of the internal ribosome entry site (IRES) of hepatitis C virus (HCV), poliovirus (PV), encephalomyocarditis virus (EMCV), and the S. cerevisiae TFIID gene...
  54. MOLECULAR AND STRUCTURAL APPROACHES TO ANTIVIRALS
    JAMES HOGLE; Fiscal Year: 1999
    ..of structure- based methods to design antiviral agents targeting several well- characterized target proteins of poliovirus, including the capsid itself, the viral RNA-dependent RNA polymerase (3D), and a protease-polymerase precursor (..
  55. REPLICATION OF VIRULENT AND ONCOGENIC VIRUSES
    ROBERT THACH; Fiscal Year: 1992
    ..Subsequently, the genome of poliovirus, and perhaps other viruses, will be mapped in the same way...
  56. RNA Replicons to Enhance Nasal Vaccines
    CASEY MORROW; Fiscal Year: 2004
    ..In previous studies from our laboratory as well as others, the use of RNA vectors (replicons) based on poliovirus or Semliki Forest Virus (SFV) have established the potential of RNA immunization...
  57. EXPRESSION OF FUNCTIONAL POLIOVIRUS REPLICATION PROTEINS
    CASEY MORROW; Fiscal Year: 2002
    The overall objective of this research proposal is to delineate at the molecular level the different steps in poliovirus encapsidation...
  58. CONTROL OF TRANSLATION BY POLIOVIRUS PROTEINASE
    Richard Lloyd; Fiscal Year: 1999
    The aim of this research is to understand the mechanism by which poliovirus specifically inactivates translation of cellular mRNA while allowing efficient translation of virus RNA in infected HeLa cells, an event known as host cell ..
  59. BIOLOGY OF C CLUSTER COXSACKIEVIRUSES
    Eckard Wimmer; Fiscal Year: 2000
    ..coxsackievirus (C-CAV), which is the group that are defined on the basis of sequence similarities as containing poliovirus, and various coxsackieviruses, including CAV21 and CAV24...
  60. Temperature-dependent, Intermediate Particles from the Entry of Picornaviruses in
    David Belnap; Fiscal Year: 2009
    ..For poliovirus, the type member of the picornavirus family, the virus-receptor interaction induces temperature-dependent ..
  61. Genetically stable Picornavirus HIV Immunization vector
    Matthias Gromeier; Fiscal Year: 2004
    ..We are pursuing an ambitious program to generate live attenuated poliovirus vaccine vectors expressing immunogenic material derived of HIV...
  62. POLIOVIRUS USING INFECTIOUS VIRAL CDNA CLONES
    VINCENT RACANIELLO; Fiscal Year: 1992
    ..in this proposal is to employ a combination of genetic and molecular biological approaches for studying poliovirus replication and pathogenesis...
  63. Improved data collection for FEI TF20 and Philips CM12 electron microscopes
    Esther Bullitt; Fiscal Year: 2009
    ..with its binding receptor, LDLR, 4) studies on the role of protein oligomerization for the replication of poliovirus RNA, including studies on the 3Dpol polymerase in isolation and tethered to membranes via the protein 3AB, 5) ..