- Multiplex real-time PCR for detection of respiratory tract infections
Department of Infection, The Sahlgrenska Academy, Goteborg University, 416 85 Goteborg, Sweden
J Clin Virol 41:53-6. 2008
..Broad diagnostics of respiratory infection by molecular assays has not yet won acceptance due to technical difficulties and high costs...
- Evolution of cell recognition by viruses
, , Cantoblanco, 28049 Madrid, Spain
Science 292:1102-5. 2001
Evolution of receptor specificity by viruses has several implications for viral pathogenesis, host range, virus-mediated gene targeting, and viral adaptation after organ transplantation and xenotransplantation, as well as for the ..
- Host defense, viruses and apoptosis
G N Barber
Department of Microbiology and Immunology and Sylvester Comprehensive Cancer Center, University of Miami School of Medicine, FL 33136, USA
Cell Death Differ 8:113-26. 2001
..This likelihood is additionally underlined by the elaborate strategies developed by viruses to inhibit IFN-antiviral function and the mechanisms of cell death.
- Viral metagenomics
Eric L Delwart
Blood Systems Research Institute, University of California, San Francisco, CA 94118, USA
Rev Med Virol 17:115-31. 2007
Characterisation of new viruses is often hindered by difficulties in amplifying them in cell culture, limited antigenic/serological cross-reactivity or the lack of nucleic acid hybridisation to known viral sequences...
- Viruses, endoplasmic reticulum stress, and interferon responses
Department of Microbiology and Immunology, College of Medicine, The University of Illinois at Chicago, 835 South Wolcott Avenue, Chicago, IL 60612, USA
Cell Death Differ 13:393-403. 2006
..Thus, to establish productive infection, viruses have evolved mechanisms to overcome the deleterious effects of PKR...
- Functional metagenomic profiling of nine biomes
Elizabeth A Dinsdale
Department of Biology, San Diego State University, San Diego, California 92182, USA
Nature 452:629-32. 2008
- Rates of evolutionary change in viruses: patterns and determinants
Center for Infectious Disease Dynamics, Department of Biology, Mueller Laboratory, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
Nat Rev Genet 9:267-76. 2008
..We review our current knowledge of the rates of mutation and substitution, as well as their determinants, in RNA viruses, DNA viruses and retroviruses...
- Marine viruses--major players in the global ecosystem
Curtis A Suttle
University of British Columbia, Departments of Earth and Ocean Sciences, Botany, and Microbiology and Immunology, 1461 BioSciences, 6270 University Boulevard, Vancouver, British Columbia V6T 1Z4, Canada
Nat Rev Microbiol 5:801-12. 2007
b>Viruses are by far the most abundant 'lifeforms' in the oceans and are the reservoir of most of the genetic diversity in the sea...
- Human CD14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors
U838 INSERM, Universite Paris Descartes, Paris, France
Immunity 33:375-86. 2010
..Instead, they selectively produced TNF-α, IL-1β, and CCL3 in response to viruses and immune complexes containing nucleic acids, via a proinflammatory TLR7-TLR 8-MyD88-MEK pathway...
- Viruses in the faecal microbiota of monozygotic twins and their mothers
Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, Missouri 63108, USA
Nature 466:334-8. 2010
..These results indicate that a predatory viral-microbial dynamic, manifest in a number of other characterized environmental ecosystems, is notably absent in the very distal intestine...
- Viral metagenomics
Robert A Edwards
Department of Biology, Center for Microbial Sciences, San Diego State University, 5500 Campanile Drive, San Diego, California 92182, USA
Nat Rev Microbiol 3:504-10. 2005
b>Viruses, most of which infect microorganisms, are the most abundant biological entities on the planet...
- Rapidly evolving CRISPRs implicated in acquired resistance of microorganisms to viruses
Gene W Tyson
Department of Environmental Science, Policy and Management, University of California, Berkeley, Berkeley, CA 94720, USA
Environ Microbiol 10:200-7. 2008
Recent experimental evidence has demonstrated that bacteria acquire resistance to viruses by incorporation of short transcribed nucleotide sequences into regions of clustered regularly interspaced short palindromic repeats (CRISPR)...
- The landscape of human proteins interacting with viruses and other pathogens
Matthew D Dyer
Genetics, Bioinformatics, and Computational Biology Program, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America
PLoS Pathog 4:e32. 2008
..with bacterial pathogens, viral pathogens, and those interacting with multiple bacteria and with multiple viruses. Gene Ontology functions enriched in these sets reveal a number of processes, such as cell cycle regulation, ..
- Metagenomics and future perspectives in virus discovery
John L Mokili
Department of Biology, San Diego State University, San Diego, CA 92182, USA
Curr Opin Virol 2:63-77. 2012
..of the virus in cell culture, a proven technique responsible for the identification of the vast majority of viruses known to date...
- Expanding roles for CD4⁺ T cells in immunity to viruses
Susan L Swain
Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue N, Worcester, Massachusetts 01655, USA
Nat Rev Immunol 12:136-48. 2012
..other lymphocytes, and have described previously unappreciated functions for memory CD4(+) T cells in immunity to viruses. Here, we review the full range of antiviral functions of CD4(+) T cells, discussing the activities of these ..
- Endogenous viruses: insights into viral evolution and impact on host biology
Department of Biology, University of Texas, Arlington, Texas 76016, USA
Nat Rev Genet 13:283-96. 2012
..Surprisingly, it appears that not just retroviruses but almost all types of viruses can become endogenous...
- Activation of host pattern recognition receptors by viruses
School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland
Curr Opin Microbiol 13:503-7. 2010
..Another novel mechanism of viral DNA recognition unveiled, leading to proinflammatory cytokine production, involves the PYHIN family member AIM2...
- Host range and emerging and reemerging pathogens
Mark E J Woolhouse
Centre for Infectious Diseases, University of Edinburgh, Edinburgh, United Kingdom
Emerg Infect Dis 11:1842-7. 2005
..However, although zoonotic pathogens do represent the most likely source of emerging and reemerging infectious disease, only a small minority have proved capable of causing major epidemics in the human population...
- Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures
Richard E Randall
School of Biology, University of St Andrews, The North Haugh, St Andrews KY16 9ST, UK
J Gen Virol 89:1-47. 2008
..However, viruses can still replicate and cause disease in vivo, because they have some strategy for at least partially ..
- How viruses hijack cell regulation
Norman E Davey
Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
Trends Biochem Sci 36:159-69. 2011
b>Viruses, as obligate intracellular parasites, are the pathogens that have the most intimate relationship with their host, and as such, their genomes have been shaped directly by interactions with the host proteome...
- Viruses, microRNAs, and host interactions
Rebecca L Skalsky
Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, North Carolina 27710, USA
Annu Rev Microbiol 64:123-41. 2010
..Several viruses, especially herpesviruses, also encode miRNAs, and over 200 viral miRNAs have now been identified...
- The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses
Department of Tumor Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
Nat Immunol 5:730-7. 2004
Intracellular double-stranded RNA (dsRNA) is a chief sign of replication for many viruses. Host mechanisms detect the dsRNA and initiate antiviral responses...
- The fecal viral flora of wild rodents
Tung G Phan
Blood Systems Research Institute, San Francisco, California, United States of America
PLoS Pathog 7:e1002218. 2011
..We identified in decreasing frequency sequences related to the mammalian viruses families Circoviridae, Picobirnaviridae, Picornaviridae, Astroviridae, Parvoviridae, Papillomaviridae, ..
- Immunity to respiratory viruses
Jacob E Kohlmeier
Trudeau Institute, Saranac Lake, New York 12983, USA
Annu Rev Immunol 27:61-82. 2009
..The investigation of pulmonary immunity to respiratory viruses during a primary infection has demonstrated that multiple innate and adaptive immune mechanisms are necessary for ..
- Viral and microbial community dynamics in four aquatic environments
Computational Science Research Center, San Diego State University, San Diego, CA 92182 1245, USA
ISME J 4:739-51. 2010
..Microbial taxa, viral taxa, and metabolic function persist over time in stable ecosystems and both communities fluctuate in a Kill-the-Winner manner at the level of viral genotypes and microbial strains...
- Recognition of viral nucleic acids in innate immunity
Laboratory of Molecular Genetics, Institute for Virus Research, and Laboratory of Molecular Cell Biology, Graduate School of Biostudies, Kyoto University, Kyoto 606 8507, Japan
Rev Med Virol 20:4-22. 2010
..in infected cells but also regulating the induction of adaptive immunity, leading to the swift eradication of viruses. Recent advances in the identification of pathogen receptors in the innate immune system have revealed that ..
- Metagenomics for the discovery of novel human viruses
British Columbia Centre for Disease Control, Department of Pathology and Laboratory Medicine, University of British Columbia, 655 West 12th Avenue, Vancouver, BC, V5Z 4R4, Canada
Future Microbiol 5:177-89. 2010
Modern laboratory techniques for the detection of novel human viruses are greatly needed as physicians and epidemiologists increasingly deal with infectious diseases caused by new or previously unrecognized pathogens...
- Unbiased reconstruction of a mammalian transcriptional network mediating pathogen responses
Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
Science 326:257-63. 2009
..This study establishes a broadly applicable, comprehensive, and unbiased approach to reveal the wiring and functions of a regulatory network controlling a major transcriptional response in primary mammalian cells...
- Immunity and immunopathology to viruses: what decides the outcome?
Barry T Rouse
Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996 0845, USA
Nat Rev Immunol 10:514-26. 2010
Many viruses infect humans and most are controlled satisfactorily by the immune system with limited damage to host tissues...
- New dimensions of the virus world discovered through metagenomics
David M Kristensen
Stowers Institute of Medical Research, Kansas City, MO 64110, USA
Trends Microbiol 18:11-9. 2010
Metagenomic analysis of viruses suggests novel patterns of evolution, changes the existing ideas of the composition of the virus world and reveals novel groups of viruses and virus-like agents...
- Viruses manipulate the marine environment
Department of Biology, San Diego State University, San Diego, California 92182, USA
Nature 459:207-12. 2009
Marine viruses affect Bacteria, Archaea and eukaryotic organisms and are major components of the marine food web...
- Bat guano virome: predominance of dietary viruses from insects and plants plus novel mammalian viruses
Blood Systems Research Institute 270 Masonic Ave, San Francisco, CA 94118, USA
J Virol 84:6955-65. 2010
Bats are hosts to a variety of viruses capable of zoonotic transmissions...
- The fecal virome of pigs on a high-density farm
BSRI, 270 Masonic Ave, San Francisco, CA 94118, USA
J Virol 85:11697-708. 2011
..An average of 4.2 different mammalian viruses were shed by healthy piglets, reflecting a high level of asymptomatic infections...
- Phylodynamics of infectious disease epidemics
Erik M Volz
Department of Epidemiology, University of Michigan, Ann Arbor, Michigan 48109, USA
Genetics 183:1421-30. 2009
..We account for phylogenetic patterns of viruses in susceptible-infected (SI), susceptible-infected-susceptible (SIS), and susceptible-infected-recovered (SIR) ..
- Critical role for the chemokine receptor CXCR6 in NK cell-mediated antigen-specific memory of haptens and viruses
Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
Nat Immunol 11:1127-35. 2010
..NK cell memory of haptens and viruses depended on CXCR6, a chemokine receptor on hepatic NK cells that was required for the persistence of memory NK ..
- Metagenomic characterization of Chesapeake Bay virioplankton
Shellie R Bench
College of Marine and Earth Studies, University of Delaware, Newark, Delaware 19711, USA
Appl Environ Microbiol 73:7629-41. 2007
b>Viruses are ubiquitous and abundant throughout the biosphere. In marine systems, virus-mediated processes can have significant impacts on microbial diversity and on global biogeocehmical cycling...
- Metavir: a web server dedicated to virome analysis
Laboratoire Microorganismes Génome et Environnement, Clermont Universite, Universite Blaise Pascal, BP 10448, F 63000 Clermont Ferrand, France
Bioinformatics 27:3074-5. 2011
..Metavir is thus unique as a platform that allows a comprehensive virome analysis...
- Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02154, USA
Curr Opin Struct Biol 21:265-73. 2011
Nine different near-atomic resolution structures of icosahedral viruses, determined by electron cryo-microscopy and published between early 2008 and late 2010, fulfil predictions made 15 years ago that single-particle cryo-EM techniques ..
- Evolutionary conflicts between viruses and restriction factors shape immunity
Nisha K Duggal
Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington 98109, USA
Nat Rev Immunol 12:687-95. 2012
..However, viruses have evolved mechanisms that antagonize restriction factors...
- Avoiding the void: cell-to-cell spread of human viruses
The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Nat Rev Microbiol 6:815-26. 2008
..Although this route of infection is undoubtedly important, many viruses that are pathogenic for humans, including HIV-1, herpes simplex virus and measles, can also move between cells ..
- Innate host defense mechanisms of fish against viruses and bacteria
A E Ellis
FRS Marine Laboratory, PO Box 101, Victoria Road, Aberdeen AB11 9DB, Scotland, UK
Dev Comp Immunol 25:827-39. 2001
..tract is a hostile environment of acids, bile salts and enzymes able to inactivate and digest many viruses and bacteria...
- Two-stepping through time: mammals and viruses
Nicholas R Meyerson
Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Trends Microbiol 19:286-94. 2011
..Long-term interactions between viruses and their hosts often develop into genetic arms races where both parties continually jockey for evolutionary ..
- Cross-reactive CD8+ T-cell immunity between the pandemic H1N1-2009 and H1N1-1918 influenza A viruses
Protein Crystallography Unit, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Proc Natl Acad Sci U S A 107:12599-604. 2010
..evolution of immunization protocols that provide a measure of protection against unpredicted pandemic influenza viruses. Thus, it is worth exploring the potential of vaccines that incorporate peptide variants with a proven potential ..
- The airway epithelium: soldier in the fight against respiratory viruses
Division of Respiratory Medicine, Department of Pediatrics, University Children s Hospital of Bern, Inselpital, 3010 Bern, Switzerland
Clin Microbiol Rev 24:210-29. 2011
The airway epithelium acts as a frontline defense against respiratory viruses, not only as a physical barrier and through the mucociliary apparatus but also through its immunological functions...
- Viral infections of the lower respiratory tract: old viruses, new viruses, and the role of diagnosis
Andrew T Pavia
Department of Pediatrics, Division of Pediatric Infectious Diseases, University of Utah, Salt Lake City, UT 84108, USA
Clin Infect Dis 52:S284-9. 2011
..of the lower respiratory tract cause an enormous disease burden in children, and the role of respiratory viruses in serious lower respiratory tract infections (LRTIs) in older adults is increasingly appreciated...
- Mechanisms of action of ribavirin against distinct viruses
Jason D Graci
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA
Rev Med Virol 16:37-48. 2006
The nucleoside analogue ribavirin has antiviral activity against many distinct viruses both in vitro and in vivo. Five distinct mechanisms have been proposed to explain the antiviral properties of ribavirin...
- Reprogrammed viruses as cancer therapeutics: targeted, armed and shielded
Department of Molecular Medicine, Rochester, MayoClinic, Minnesota 55905, USA
Nat Rev Microbiol 6:529-40. 2008
..b>Viruses can be reprogrammed into oncolytic vectors by combining three types of modification: targeting, arming and ..
- Selective autophagy and viruses
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX USA
Autophagy 7:260-5. 2011
..However, until recently, there have been virtually no studies on the selective autophagy of viruses, although they are perhaps one of the most ubiquitous unwanted constituents in human cells...
- Viruses and endosome membrane dynamics
Department of Biochemistry, University of Geneva, Geneva, Switzerland
Curr Opin Cell Biol 21:582-8. 2009
..It seems that viruses can enter and infect cells through most of, if not all, endocytic routes, having evolved different, sometimes ..
- Viral disorder or disordered viruses: do viral proteins possess unique features?
Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Protein Pept Lett 17:932-51. 2010
..b>Viruses, these "organisms at the edge of life", have uniquely evolved to be highly adaptive for fast change in their ..
- Paleovirology - ghosts and gifts of viruses past
Maulik R Patel
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle WA USA
Curr Opin Virol 1:304-9. 2011
The emerging field of paleovirology aims to study the evolutionary age and impact of ancient viruses (paleoviruses) on host biology...
- New genotype of dengue type 3 virus circulating in Brazil and Colombia showed a close relationship to old Asian viruses
Victor Hugo Aquino
Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo, Brasil
PLoS ONE 4:e7299. 2009
Dengue type 3 genotype V viruses have been recently detected in Brazil and Colombia. In this study, we described another Brazilian isolate belonging to this genotype...
- Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People s Republic of China
J Virol 86:10999-1012. 2012
Bats are natural hosts for a large variety of zoonotic viruses. This study aimed to describe the range of bat viromes, including viruses from mammals, insects, fungi, plants, and phages, in 11 insectivorous bat species (216 bats in total)..
- Single-particle tracking as a quantitative microscopy-based approach to unravel cell entry mechanisms of viruses and pharmaceutical nanoparticles
Department of Chemistry, Ludwig Maximilians Universitat Munchen, Munich, Germany
Mol Ther 19:1199-211. 2011
..Such an analysis provides important information regarding the uptake pathway and location of viruses and nanoparticles...
- Nanobodies®: new ammunition to battle viruses
Ablynx NV, Ghent, Belgium
Antiviral Res 92:389-407. 2011
..be described in the context of different experimental therapeutic applications of Nanobodies against different viruses: HIV, Hepatitis B virus, influenza virus, Respiratory Syncytial virus, Rabies virus, FMDV, Poliovirus, Rotavirus, ..
- Frequent detection of respiratory viruses without symptoms: toward defining clinically relevant cutoff values
Rogier R Jansen
Department of Medical Microbiology1 and Department of Pediatrics, 2 Academical Medical Centre, University of Amsterdam, Amsterdam, Netherlands
J Clin Microbiol 49:2631-6. 2011
Highly sensitive techniques, such as PCR, have greatly improved the detection of respiratory viruses. However, the sensitivity of PCR tests also complicates clinical interpretation, as the presence of small amounts of viral targets may ..
- Viruses and autophagy
Sagar B Kudchodkar
Department of Internal Medicine, University of Texas Southwestern Medical Center at Dallas, TX, USA
Rev Med Virol 19:359-78. 2009
..As intracellular parasites, during the course of an infection, viruses encounter autophagy and interact with the proteins that execute this process...
- To kill or be killed: how viruses interact with the cell death machinery
Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden
J Intern Med 267:473-82. 2010
..b>Viruses are found wherever there is life and have probably existed since living cells first evolved...
- Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children
K H Chan
Department of Microbiology, The University of Hong Kong and Queen Mary Hospital, Hong Kong SAR, China
J Clin Virol 42:65-9. 2008
..The quality of clinical specimens is a crucial determinant for virological diagnosis...
- The evolution of RNAi as a defence against viruses and transposable elements
Darren J Obbard
Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Ashworth Laboratories, King s Buildings, Edinburgh EH9 3JT, UK
Philos Trans R Soc Lond B Biol Sci 364:99-115. 2009
RNA interference (RNAi) is an important defence against viruses and transposable elements (TEs)...
- Heterologous immunity between viruses
Raymond M Welsh
Department of Pathology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Immunol Rev 235:244-66. 2010
..Here, we discuss the nature of T-cell cross-reactivity and describe matrices of epitopes from different viruses eliciting cross-reactive CD8(+) T-cell responses...
- Ten reasons to exclude viruses from the tree of life
Unité d Ecologie, Systematique et Evolution, UMR CNRS 8, 079, Universite Paris Sud, Batiment 360, 91405 Orsay Cedex, France
Nat Rev Microbiol 7:306-11. 2009
When viruses were discovered, they were accepted as missing links between the inert world and living organisms. However, this idea was soon abandoned as information about their molecular parasitic nature accumulated...
- Transneuronal circuit tracing with neurotropic viruses
Edward M Callaway
The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Curr Opin Neurobiol 18:617-23. 2008
Because neurotropic viruses naturally traverse neural pathways, they are extremely valuable for elucidating neural circuits. Naturally occurring herpes and rabies viruses have been used for transneuronal circuit tracing for decades...
- Viruses as vaccine vectors for infectious diseases and cancer
Simon J Draper
The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK
Nat Rev Microbiol 8:62-73. 2010
Recent developments in the use of viruses as vaccine vectors have been facilitated by a better understanding of viral biology. Advances occur as we gain greater insight into the interrelationship of viruses and the immune system...
- Prevalence of human respiratory viruses in adults with acute respiratory tract infections in Beijing, 2005-2007
Dr Christophe Mérieux Laboratory, IPB, CAMS Fondation Mérieux and State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology IPB, Chinese Academy of Medical Sciences CAMS, Beijing, China
Clin Microbiol Infect 15:1146-53. 2009
To determine the aetiological role and epidemiological profile of common respiratory viruses in adults with acute respiratory tract infections (ARTIs), a 2-year study was conducted in Beijing, China, from May 2005 to July 2007...
- Natural killer cells, viruses and cancer
Department of Microbiology and Immunology and the Cancer Research Institute, University of California, San Francisco, 94143-0414, USA
Nat Rev Immunol 1:41-9. 2001
..Here, we review the emerging evidence that natural killer cells have an important role in vivo in immune defence...
- Viruses and apoptosis
GeminX Biotechnologies Inc, Montreal, Quebec, Canada
Annu Rev Microbiol 53:577-628. 1999
..In addition to inducing immune and inflammatory responses, infection by most viruses triggers apoptosis or programmed cell death of the infected cell...
- Metagenomics and the molecular identification of novel viruses
Department of Veterinary Medicine, University of Cambridge, Cambridge, UK
Vet J 190:191-8. 2011
There have been rapid recent developments in establishing methods for identifying and characterising viruses associated with animal and human diseases...
- Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay
Suk Ran Kim
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
J Virol Methods 156:111-6. 2009
..Seoul, Korea) for simultaneous detection and identification of 12 respiratory viruses using two primer mixes...
- Are viruses a source of new protein folds for organisms? - Virosphere structure space and evolution
Estonian Biocentre, Tartu, Estonia
Bioessays 33:626-35. 2011
..the virosphere into the global picture of protein structure space reveals that 63 protein domain superfamilies in viruses do not have any structural and evolutionary relatives in modern cellular organisms...
- A biophysical protein folding model accounts for most mutational fitness effects in viruses
C Scott Wylie
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 108:9916-21. 2011
..Compensatory mutations are common in small populations with high mutation rates. More broadly, we conclude that interplay between biophysical and population genetic forces shapes the DFE...
- Detection of new respiratory viruses in hospitalized infants with bronchiolitis: a three-year prospective study
Pediatrics Department, Severo Ochoa Hospital, Madrid, Spain
Acta Paediatr 99:883-7. 2010
We have designed a study with the objective of describing the clinical impact of other viruses different from the respiratory syncytial virus (RSV) in hospitalized infants with bronchiolitis.
- Real-time quantitative PCR assays for detection and monitoring of pathogenic human viruses in immunosuppressed pediatric patients
Division of Molecular Microbiology and Development of Genetic Diagnostics, Children s Cancer Research Institute, Vienna, Austria
J Clin Microbiol 42:5189-98. 2004
..PCR assays based on TaqMan technology has been developed for the detection and monitoring of 16 different viruses and virus families including human polyomaviruses BK virus and JC virus, human herpesviruses 6, 7, and 8, human ..
- High burden of non-influenza viruses in influenza-like illness in the early weeks of H1N1v epidemic in France
Universite Paris Diderot, Sorbonne Paris Cité, Microbiology Department, Saint Louis Hospital, APHP, Paris, France
PLoS ONE 6:e23514. 2011
..Clinical observations are of little help to recognise myxovirus infection and implement appropriate prevention measures. The limited use of molecular tools underestimates the role of other common pathogens...
- Marine viruses and global climate change
Department of Marine Sciences, Polytechnic University of Marche, Ancona, Italy
FEMS Microbiol Rev 35:993-1034. 2011
..Changing climate has direct and indirect consequences on marine viruses, including cascading effects on biogeochemical cycles, food webs, and the metabolic balance of the ocean...
- Detection of respiratory viruses by molecular methods
James B Mahony
Regional Virology Laboratory, St Joseph s Healthcare Hamilton, Hamilton, Ontario, Canada
Clin Microbiol Rev 21:716-47. 2008
..With the discovery of six new respiratory viruses since 2000, laboratories are faced with the challenge of detecting up to 19 different viruses that cause acute ..
- VISA is an adapter protein required for virus-triggered IFN-beta signaling
Liang Guo Xu
Department of Immunology, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado 80206, USA
Mol Cell 19:727-40. 2005
..These findings suggest that VISA is critically involved in both virus-triggered TLR3-independent and TLR3-mediated antiviral IFN signaling...
- Type 1 interferons and the virus-host relationship: a lesson in détente
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
Science 312:879-82. 2006
..host's arsenal of antimicrobial factors, the type 1 interferons (IFNs) induce potent defense mechanisms against viruses and are key in the host-virus standoff...
- Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study
Laboratory Medicine, Memorial Medical Center, Springfield, Illinois 62781, USA
J Clin Microbiol 38:2824-8. 2000
To assess the expected benefits of rapid reporting of respiratory viruses, we compared patients whose samples were processed using standard techniques such as enzyme immunoassays, shell vial assays, and culture tube assays (year 1) to ..
- Association of bacteria and viruses with wheezy episodes in young children: prospective birth cohort study
The Copenhagen Prospective Studies on Asthma in Childhood, Health Sciences, University of Copenhagen, and The Danish Pediatric Asthma Centre, Copenhagen University Hospital, Gentofte, Ledreborg Alle 34, DK 2820 Gentofte, Copenhagen, Denmark
BMJ 341:c4978. 2010
..To study the association between wheezy symptoms in young children and the presence of bacteria in the airways...
- Nasal swab versus nasopharyngeal aspirate for isolation of respiratory viruses
Department of Pediatrics, Turku University Hospital Department of Virology, University of Turku, Turku, Finland
J Clin Microbiol 40:4337-9. 2002
To determine the usefulness of nasal swabs as a simple method for detection of respiratory viruses, we compared nasal swabs and nasopharyngeal aspirates obtained at the same time from the opposite nostrils of 230 children with upper ..
- Synthetic viruses: a new opportunity to understand and prevent viral disease
Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA
Nat Biotechnol 27:1163-72. 2009
..Although only a few applications of virus synthesis have been described as yet, key recent findings have been the resurrection of the 1918 influenza virus and the generation of codon- and codon pair-deoptimized polioviruses...
- Viruses and the autophagy machinery
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, USA
Cell Cycle 9:1295-1307. 2010
..Our understanding of the fascinating relationship between viruses and the autophagy machinery is still in its infancy but it is clear that autophagy is a newly recognized facet of ..
- Metagenomic analysis of viruses in reclaimed water
College of Marine Science, University of South Florida, St Petersburg, Florida, USA
Environ Microbiol 11:2806-20. 2009
..This study characterized the viral community found in reclaimed water and compared it with viruses in potable water...
- Modulation of natural killer cell activity by viruses
Vanda Juranic Lisnic
Department for Histology and Embryology and Center for Proteomics, Faculty of Medicine, University of Rijeka, B Branchetta 20, 51000 Rijeka, Croatia
Curr Opin Microbiol 13:530-9. 2010
..These mechanisms include not only virally encoded immunoevasion proteins but also viral miRNA. Moreover, the evidence has been accumulated supporting the role of viral immunoevasion of NK cells in viral pathogenesis in vivo...
- Mechanisms for enveloped virus budding: can some viruses do without an ESCRT?
Benjamin J Chen
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
Virology 372:221-32. 2008
Many enveloped viruses complete their replication cycle by forming vesicles that bud from the plasma membrane...
- Optimization of procedures for counting viruses by flow cytometry
Corina P D Brussaard
Department of Biological Oceanography, Royal Netherlands Institute for Sea Research, NL 1790 AB Den Burg, The Netherlands
Appl Environ Microbiol 70:1506-13. 2004
..acid stains, in combination with flow cytometric techniques, has allowed the identification and enumeration of viruses in aquatic systems...
- Tentative characterization of new environmental giant viruses by MALDI-TOF mass spectrometry
Bernard La Scola
Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille and URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Intervirology 53:344-53. 2010
..polyphaga Mimivirus relatives are common in the environment; however, only three Acanthamoeba-growing giant viruses have been isolated from hundreds of environmental samples...
- Innate immune recognition of viruses and viral vectors
Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
Hum Gene Ther 20:293-301. 2009
..in immunology have suggested a crucial role for the innate immune system in the induction of immune responses to viruses. Thus, a better understanding of the mechanisms by which the host's innate immune system recognizes viruses and ..
- A sensitive assay for virus discovery in respiratory clinical samples
Michel de Vries
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands
PLoS ONE 6:e16118. 2011
..Eighteen nasopharyngeal swabs were analysed, all containing known respiratory viruses. We could identify the proper virus in the majority of samples tested (11/18)...
- Viral etiology of acute respiratory infections with cough in infancy: a community-based birth cohort study
Pediatric Respiratory Medicine, University Children s Hospital of Berne, Berne, Switzerland
Pediatr Infect Dis J 27:100-5. 2008
..in detail the viral aetiology of ARI with such symptoms in otherwise healthy infants, including rhinoviruses and recently discovered viruses such as human metapneumovirus (HMPV), coronavirus NL63 and HKU1, and human bocavirus (HBoV).
- Eukaryotic viruses in wastewater samples from the United States
Erin M Symonds
College of Marine Science, University of South Florida, St Petersburg, Florida 33701, USA
Appl Environ Microbiol 75:1402-9. 2009
Human fecal matter contains a large number of viruses, and current bacterial indicators used for monitoring water quality do not correlate with the presence of pathogenic viruses...
- Plant microRNAs and their role in defense against viruses: a bioinformatics approach
Alvaro L Pérez-Quintero
Universidad Nacional de Colombia, Bogota, Departamento de Biologia, Bogota D C Colombia
BMC Plant Biol 10:138. 2010
..In this work, a bioinformatics approach was taken to test whether plant miRNAs from six species could have antiviral activity by targeting the genomes of plant infecting viruses.
- Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children
Infectious Disease Research Center of the CHUQ CHUL and Laval University, Sainte Foy, Quebec, Canada
J Clin Microbiol 47:743-50. 2009
..We developed and compared two assays that allow the detection of up to 23 different respiratory viruses that frequently infect children...
- The source of self: genetic parasites and the origin of adaptive immunity
Luis P Villarreal
Center for Virus Research, Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
Ann N Y Acad Sci 1178:194-232. 2009
Stable colonization of the host by viruses (genetic parasites) can alter the systems of host identity and provide immunity against related viruses...
- Evaluation of high-throughput sequencing for identifying known and unknown viruses in biological samples
Institut Pasteur, Genotyping of Pathogens and Public Health Platform, 28 rue du Docteur Roux, F 75015 Paris, France
J Clin Microbiol 49:3268-75. 2011
..a large number of short sequence reads from uncloned DNA and has rapidly become a major tool for identifying viruses in biological samples, and in particular when the target sequence is undefined...
- What have RNAi screens taught us about viral-host interactions?
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Curr Opin Microbiol 12:446-52. 2009
..This has led to the discovery of hundreds of new factors, and has increased our knowledge of the host factors that impact viral infection and highlighted the cellular pathways at play...
- Atomic force microscopy in imaging of viruses and virus-infected cells
Yurii G Kuznetsov
Department of Molecular Biology and Biochemistry, University of California, Irvine, 560 Steinhaus Hall, Irvine, CA 92697 3900, USA
Microbiol Mol Biol Rev 75:268-85. 2011
..Capsids of icosahedral viruses and the icosahedral capsids of enveloped viruses have been seen at high resolution, in some cases sufficiently ..
- Viral and cellular microRNAs as determinants of viral pathogenesis and immunity
Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, USA
Cell Host Microbe 3:375-87. 2008
..Here, we discuss what is known about how viral and cellular miRNAs influence viral replication and pathogenic potential through their regulation of viral mRNAs or by reshaping cellular gene expression...
- Respiratory viruses in bronchoalveolar lavage: a hospital-based cohort study in adults
University Hospitals of Geneva, Switzerland
Thorax 64:399-404. 2009
The epidemiology of respiratory viruses and their potential clinical impact when recovered in lower respiratory specimens has not been established in the hospital setting...
- Predominance and circulation of enteric viruses in the region of Greater Cairo, Egypt
Aziza H Kamel
Laboratory of Virology, National Reference Center for Enteric Viruses, Public Hospital of Dijon, Dijon, France
J Clin Microbiol 47:1037-45. 2009
The circulation of enteric viruses among the population of Cairo, Egypt, between March 2006 and February 2007 was studied. At least one virus was detected in 50% of fecal samples, 57...