Genomes and Genes
escherichia coli o157
Summary: A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. Recently, a strain from this serogroup, serotype H7 which produces Shiga-like toxins, has been linked to human disease outbreaks resulting from contamination of foods from bovine origin. This serogroup causes hemorrhagic and HEMOLYTIC-UREMIC SYNDROME and predominantly hemorrhagic diarrhea and GASTROENTERITIS in children.
Publications310 found, 100 shown here
- Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNetEfrain M Ribot
Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
Foodborne Pathog Dis 3:59-67. 2006Standardized rapid pulsed-field gel electrophoresis (PFGE) protocols for the subtyping of Escherichia coli O157:H7, Salmonella serotypes, and Shigella species are described...
- Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndromePhillip I Tarr
Division of Gastroenterology, Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, Campus Box 8208, 660 South Euclid Avenue, St Louis, MO 63110, USA
Lancet 365:1073-86. 2005..The vascular injury leading to HUS is likely to be well under way by the time infected patients seek medical attention for diarrhoea. The best way to prevent HUS is to prevent primary infection with Shiga-toxin-producing bacteria...
- Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coliYoshitoshi Ogura
Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, 5200 Kiyotake, Miyazaki 889 1692, Japan
Proc Natl Acad Sci U S A 106:17939-44. 2009..Our data demonstrate how E. coli strains of different phylogenies can independently evolve into EHECs, providing unique insights into the mechanisms underlying the parallel evolution of complex virulence systems in bacteria...
- Variation in virulence among clades of Escherichia coli O157:H7 associated with disease outbreaksShannon D Manning
Microbial Evolution Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824, USA
Proc Natl Acad Sci U S A 105:4868-73. 2008b>Escherichia coli O157:H7, a toxin-producing food and waterborne bacterial pathogen, has been linked to large outbreaks of gastrointestinal illness for more than two decades. E...
- Genomic anatomy of Escherichia coli O157:H7 outbreaksMark Eppinger
Department of Microbiology and Immunology, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Proc Natl Acad Sci U S A 108:20142-7. 2011The rapid emergence of Escherichia coli O157:H7 from an unknown strain in 1982 to the dominant hemorrhagic E...
- Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982-2002Josefa M Rangel
Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Emerg Infect Dis 11:603-9. 2005b>Escherichia coli O157:H7 causes 73,000 illnesses in the United States annually. We reviewed E. coli O157 outbreaks reported to Centers for Disease Control and Prevention (CDC) to better understand the epidemiology of E. coli O157. E...
- Genome sequence of enterohaemorrhagic Escherichia coli O157:H7N T Perna
Genome Center of Wisconsin, and Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA
Nature 409:529-33. 2001The bacterium Escherichia coli O157:H7 is a worldwide threat to public health and has been implicated in many outbreaks of haemorrhagic colitis, some of which included fatalities caused by haemolytic uraemic syndrome...
- Escherichia coli O157Hugh Pennington
University of Aberdeen, Aberdeen, UK
Lancet 376:1428-35. 2010b>Escherichia coli O157 is an uncommon but serious cause of gastroenteritis...
- Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptomsAlexander W Friedrich
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Wurzburg, Germany
J Infect Dis 185:74-84. 2002..stx2c-positive STEC isolates can cause HUS, but the presence of stx2d or stx2e may predict a milder disease with a minimal risk of HUS...
- Risk of hemolytic uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 enteritis: a meta-analysisNasia Safdar
Department of Medicine, University of Wisconsin Medical School and University of Wisconsin Hospital and Clinics, Madison, WI 53792 5158, USA
JAMA 288:996-1001. 2002The use of antibiotics for treatment of Escherichia coli O157:H7 infection has become controversial since a recent small study found that it may increase the risk of hemolytic uremic syndrome (HUS)...
- Derivation of Escherichia coli O157:H7 from its O55:H7 precursorZhemin Zhou
Tianjin Economic Technological Development Area School of Biological Sciences and Biotechnology, Nankai University, Tianjin, China
PLoS ONE 5:e8700. 2010..Our findings also suggest that E. coli has a much lower frequency of recombination relative to mutation than was observed in a comparable study of a Vibrio cholerae lineage...
- Analysis of the genome of the Escherichia coli O157:H7 2006 spinach-associated outbreak isolate indicates candidate genes that may enhance virulenceBridget R Kulasekara
Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98195, USA
Infect Immun 77:3713-21. 2009In addition to causing diarrhea, Escherichia coli O157:H7 infection can lead to hemolytic-uremic syndrome (HUS), a severe disease characterized by hemolysis and renal failure. Differences in HUS frequency among E...
- Super-shedding and the link between human infection and livestock carriage of Escherichia coli O157Margo Chase-Topping
Centre for Infectious Diseases, University of Edinburgh, Kings Buildings, Edinburgh, EH9 3JT, UK
Nat Rev Microbiol 6:904-12. 2008Cattle that excrete more Escherichia coli O157 than others are known as super-shedders. Super-shedding has important consequences for the epidemiology of E...
- Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7Roger Thilmony
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
Plant J 46:34-53. 2006..transcriptome associated with basal defense to Pst DC3000 hrp mutants and the human pathogenic bacterium Escherichia coli O157:H7...
- Mosaic structure of Shiga-toxin-2-encoding phages isolated from Escherichia coli O157:H7 indicates frequent gene exchange between lambdoid phage genomesB K Johansen
Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, PO Box 8146 Dep, N-0033 Oslo, Norway
Microbiology 147:1929-36. 2001Shiga-toxin-2 (stx(2))-encoding bacteriophages were isolated from Norwegian Escherichia coli O157:H7 isolates of cattle and human origin...
- Hemolytic uremic syndrome and death in persons with Escherichia coli O157:H7 infection, foodborne diseases active surveillance network sites, 2000-2006L Hannah Gould
Enteric Diseases Epidemiology Branch, National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
Clin Infect Dis 49:1480-5. 2009..uremic syndrome (HUS) is a life-threatening illness usually caused by infection with Shiga toxin-producing Escherichia coli O157 (STEC O157)...
- Lineage and host source are both correlated with levels of Shiga toxin 2 production by Escherichia coli O157:H7 strainsYongxiang Zhang
Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, AB T1J 3Z4, Canada
Appl Environ Microbiol 76:474-82. 2010b>Escherichia coli O157:H7 strains fall into three major genetic lineages that differ in their distribution among humans and cattle. Several recent studies have reported differences in the expression of virulence factors between E...
- Risk factors for the hemolytic uremic syndrome in children infected with Escherichia coli O157:H7: a multivariable analysisCraig S Wong
Department of Pediatrics, Division of Nephrology, University of New Mexico Children s Hospital, Albuquerque, USA
Clin Infect Dis 55:33-41. 2012b>Escherichia coli O157:H7 is the leading cause of hemolytic uremic syndrome (HUS). Risk factors for development of this complication warrant identification.
- Infection by Shiga toxin-producing Escherichia coli: an overviewMohamed A Karmali
Laboratory for Foodborne Zoonoses, Health Canada, 110 Stone Road West, Guelph, Ontario, Canada, N1G 3W4
Mol Biotechnol 26:117-22. 2004..The recent sequencing of genomes of two epidemic E. coli O157 strains has revealed novel pathogenicity islands which will likely provide new insights into the virulence of these bacteria...
- Enteropathogenic and enterohemorrhagic Escherichia coli virulence gene regulationJay L Mellies
Biology Department, Reed College, 3203 S E Woodstock Boulevard, Portland, OR 97202, USA
Infect Immun 75:4199-210. 2007
- Toxin gene expression by shiga toxin-producing Escherichia coli: the role of antibiotics and the bacterial SOS responseP T Kimmitt
The Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom
Emerg Infect Dis 6:458-65. 2000..These features and the use of SOS-inducing antibiotics in clinical practice and animal husbandry may account for the recent emergence of STEC disease...
- Fresh fruit and vegetables as vehicles for the transmission of human pathogensCedric N Berger
Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, UK
Environ Microbiol 12:2385-97. 2010....
- The defective prophage pool of Escherichia coli O157: prophage-prophage interactions potentiate horizontal transfer of virulence determinantsMd Asadulghani
Division of Bioenvironmental Sciences, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan
PLoS Pathog 5:e1000408. 2009..Enterohemorrhagic Escherichia coli O157 is the most striking case...
- Escherichia coli O157:H7: animal reservoir and sources of human infectionWitold A Ferens
Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 3052, USA
Foodborne Pathog Dis 8:465-87. 2011..Transmission to people occurs primarily via ingestion of inadequately processed contaminated food or water and less frequently through contact with manure, animals, or infected people...
- The effect of lemon, orange and bergamot essential oils and their components on the survival of Campylobacter jejuni, Escherichia coli O157, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus in vitro and in food systemsK Fisher
School of Health, The University of Northampton, Northampton, UK
J Appl Microbiol 101:1232-40. 2006..To investigate the effectiveness of oils and vapours of lemon (Citrus limon), sweet orange (Citrus sinensis) and bergamot (Citrus bergamia) and their components against a number of common foodborne pathogens...
- Genome-wide transposon mutagenesis reveals a role for pO157 genes in biofilm development in Escherichia coli O157:H7 EDL933Supraja Puttamreddy
Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011, USA
Infect Immun 78:2377-84. 2010Enterohemorrhagic Escherichia coli O157:H7, a world-wide human food-borne pathogen, causes mild to severe diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome...
- Bacteria-host communication: the language of hormonesVanessa Sperandio
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
Proc Natl Acad Sci U S A 100:8951-6. 2003..Given that eukaryotic cell-to-cell signaling typically occurs through hormones, and that bacterial cell-to-cell signaling occurs through QS, we speculate that QS might be a "language" by which bacteria and host cells communicate...
- Diet, Escherichia coli O157:H7, and cattle: a review after 10 yearsTodd R Callaway
Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, Agricultural Research Service, USDA, College Station, TX 77845, USA
Curr Issues Mol Biol 11:67-79. 2009..Data from researchers across North America indicate that diet does impact STEC/EHEC populations in cattle prior to slaughter; however the economic, logistic and practical impacts of dietary changes must be examined and accounted for...
- Grazing protozoa and the evolution of the Escherichia coli O157:H7 Shiga toxin-encoding prophageKaryn Meltz Steinberg
Graduate Program in Population Biology, Ecology and Evolution, Emory University, Atlanta, GA 30322, USA
Proc Biol Sci 274:1921-9. 2007Humans play little role in the epidemiology of Escherichia coli O157:H7, a commensal bacterium of cattle. Why then does E...
- Shiga toxin 2 overexpression in Escherichia coli O157:H7 strains associated with severe human diseaseMahesh Neupane
Microbial Evolution and Epidemiology Laboratory, Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States
Microb Pathog 51:466-70. 2011Variation in disease severity among Escherichia coli O157:H7 infections may result from differential expression of Shiga toxin 2 (Stx2)...
- Effects of environmental stress on stability of tandem repeats in Escherichia coli O157:H7Michael B Cooley
Produce Safety and Microbiology Research Unit, USDA Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA
Appl Environ Microbiol 76:3398-400. 2010Multilocus variable-number tandem-repeat analysis (MLVA) is used for source tracking Escherichia coli O157:H7 in agricultural environments...
- A multistate outbreak of Escherichia coli O157:H7 infections associated with consumption of mesclun lettuceE D Hilborn
Epidemiology Program Office, Centers for Disease Control and Prevention, Atlanta, GA, USA
Arch Intern Med 159:1758-64. 1999An outbreak of Escherichia coli O157:H7 infections in Connecticut and Illinois during May 28 to June 27, 1996, was investigated to determine the source of infections.
- Characterization of a ViI-like phage specific to Escherichia coli O157:H7Elizabeth M Kutter
The Evergreen State College, Olympia, WA, USA
Virol J 8:430. 2011Phage vB_EcoM_CBA120 (CBA120), isolated against Escherichia coli O157:H7 from a cattle feedlot, is morphologically very similar to the classic phage ViI of Salmonella enterica serovar Typhi...
- Antibiotic treatment of Escherichia coli O157 infection and the risk of hemolytic uremic syndrome, MinnesotaKirk E Smith
Acute Disease Investigation and Control Section, Minnesota Department of Health, St Paul, MN 55164, USA
Pediatr Infect Dis J 31:37-41. 2012Infection with Escherichia coli O157 (O157) can lead to the development of hemolytic uremic syndrome (HUS)...
- Inference of the impact of insertion sequence (IS) elements on bacterial genome diversification through analysis of small-size structural polymorphisms in Escherichia coli O157 genomesTadasuke Ooka
Department of Infectious Diseases, University of Miyazaki, Japan
Genome Res 19:1809-16. 2009..In enterohemorrhagic Escherichia coli O157, a major factor that affects genomic diversity is prophages, which generate most of the large-size ..
- Expression of receptors for verotoxin 1 from Escherichia coli O157 on bovine intestinal epitheliumD E Elaine Hoey
Department of Medical Microbiology, University of Edinburgh, UK
J Med Microbiol 51:143-9. 2002..Expression of Gb3 on the bovine intestinal epithelium, together with previously described effects, may affect EHEC colonisation in its reservoir hosts and hence the potential for distribution to man...
- Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmissionAlfredo Caprioli
Dipartimento di Sanità alimentare e animale, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
Vet Res 36:289-311. 2005..However, their occurrence can be minimised by applying high standards of hygiene in all the steps of the food production chain...
- Global regulation by horizontally transferred regulators establishes the pathogenicity of Escherichia coliHiroyuki Abe
Division of Applied Bacteriology, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
DNA Res 15:25-38. 2008..These results indicate that Ler and Pch orchestrate the transcription of virulence genes, which are captured by horizontal transfer and scattered throughout the chromosome...
- Genome evolution in major Escherichia coli O157:H7 lineagesYongxiang Zhang
Laboratory for Foodborne Zoonoses, Health Canada, Lethbridge, AB, Canada
BMC Genomics 8:121. 2007Genetic analysis of Escherichia coli O157:H7 strains has shown divergence into two distinct lineages, lineages I and II, that appear to have distinct ecological characteristics, with lineage I strains more commonly associated with human ..
- Incidence and tracking of Escherichia coli O157:H7 in a major produce production region in CaliforniaMichael Cooley
Produce Safety and Microbiology Research Unit, United States Department of Agriculture Agricultural Research Service, Western Regional Research Center, Albany, California, United States of America
PLoS ONE 2:e1159. 2007Fresh vegetables have become associated with outbreaks caused by Escherichia coli O157:H7 (EcO157)...
- Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7Marie E Fraser
Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada
J Biol Chem 279:27511-7. 2004..Any of these structural differences might result in different mechanisms of action of the two toxins and the development of hemolytic uremic syndrome upon exposure to Stx2...
- Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12T Hayashi
Department of Microbiology, Miyazaki Medical College, Kiyotake, Japan
DNA Res 8:11-22. 2001b>Escherichia coli O157:H7 is a major food-borne infectious pathogen that causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome...
- Spinach-associated Escherichia coli O157:H7 outbreak, Utah and New Mexico, 2006Juliana Grant
Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Emerg Infect Dis 14:1633-6. 2008In 2006, Utah and New Mexico health departments investigated a multistate cluster of Escherichia coli O157:H7. A case-control study of 22 case-patients found that consuming bagged spinach was significantly associated with illness (p<0...
- Increased adherence and expression of virulence genes in a lineage of Escherichia coli O157:H7 commonly associated with human infectionsGaleb S Abu-Ali
Microbial Evolution Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan, United States of America
PLoS ONE 5:e10167. 2010..Little is known, however, about how these two lineages vary with regard to phenotypic traits important for disease pathogenesis and in the expression of shared virulence genes...
- Relative nephroprotection during Escherichia coli O157:H7 infections: association with intravenous volume expansionJulie A Ake
Department of Medicine, Madigan Army Medical Center, Tacoma, Washington, USA
Pediatrics 115:e673-80. 2005....
- Mouse models of Escherichia coli O157:H7 infection and shiga toxin injectionKrystle L Mohawk
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
J Biomed Biotechnol 2011:258185. 2011b>Escherichia coli O157:H7 has been responsible for multiple food- and waterborne outbreaks of diarrhea and/or hemorrhagic colitis (HC) worldwide. More importantly, a portion of E...
- Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7G O'Flynn
Dairy Products Research Centre, Teagasc, Moorepark, Fermoy, County Cork, Ireland
Appl Environ Microbiol 70:3417-24. 2004b>Escherichia coli O157:H7 is an endemic pathogen causing a variety of human diseases including mild diarrhea, hemorrhagic colitis, hemolytic-uremic syndrome, and thrombotic thrombocytopenic purpura...
- Quorum-sensing Escherichia coli regulator A: a regulator of the LysR family involved in the regulation of the locus of enterocyte effacement pathogenicity island in enterohemorrhagic E. coliVanessa Sperandio
Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
Infect Immun 70:3085-93. 2002..Similar results were also observed with a qseA mutant of EPEC. The QseA regulator is part of the regulatory cascade that regulates EHEC and EPEC virulence genes by quorum sensing...
- Phylogeny of Shiga toxin-producing Escherichia coli O157 isolated from cattle and clinically ill humansJames L Bono
United States Department of Agriculture, Agricultural Research Service, US Meat Animal Research Center, Clay Center, Nebraska, USA
Mol Biol Evol 29:2047-62. 2012Cattle are a major reservoir for Shiga toxin-producing Escherichia coli O157 (STEC O157) and harbor multiple genetic subtypes that do not all associate with human disease. STEC O157 evolved from an E...
- Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and foodCarl M Schroeder
Department of Nutrition and Food Science, 3304 Marie Mount Hall, University of Maryland, College Park, MD 20742, USA
Appl Environ Microbiol 68:576-81. 2002A total of 361 Escherichia coli O157 isolates, recovered from humans, cattle, swine, and food during the years 1985 to 2000, were examined to better understand the prevalence of antimicrobial resistance among these organisms...
- Genome signatures of Escherichia coli O157:H7 isolates from the bovine host reservoirMark Eppinger
Institute for Genome Sciences IGS, University of Maryland, School of Medicine, Baltimore, MD 21201, USA
Appl Environ Microbiol 77:2916-25. 2011Cattle comprise a main reservoir of Shiga toxin-producing Escherichia coli O157:H7 (STEC)...
- Bile salts induce resistance to polymyxin in enterohemorrhagic Escherichia coli O157:H7Julianne V Kus
Department of Chemistry and Biology, Ryerson University, 350 Victoria St, Toronto, ON M5B 2K3, Canada
J Bacteriol 193:4509-15. 2011..Microarray analysis of enterohemorrhagic Escherichia coli O157:H7 (EHEC) treated with bile salts revealed upregulation of genes for an efflux system (acrAB), a two-..
- Bacteriophages reduce experimental contamination of hard surfaces, tomato, spinach, broccoli, and ground beef by Escherichia coli O157:H7Tamar Abuladze
Intralytix, Inc, Baltimore, MD 21202, USA
Appl Environ Microbiol 74:6230-8. 2008A bacteriophage cocktail (designated ECP-100) containing three Myoviridae phages lytic for Escherichia coli O157:H7 was examined for its ability to reduce experimental contamination of hard surfaces (glass coverslips and gypsum boards), ..
- Human serum amyloid P component protects against Escherichia coli O157:H7 Shiga toxin 2 in vivo: therapeutic implications for hemolytic-uremic syndromeGlen D Armstrong
Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada
J Infect Dis 193:1120-4. 2006..2 causes hemolytic-uremic syndrome (HUS), an intractable and often fatal complication of enterohemorrhagic Escherichia coli O157:H7 infection...
- Contributions of O island 48 to adherence of enterohemorrhagic Escherichia coli O157:H7 to epithelial cells in vitro and in ligated pig ileal loopsXianhua Yin
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G2W1
Appl Environ Microbiol 75:5779-86. 2009..These data suggest that Te(r), Iha, and urease may contribute to EHEC O157:H7 pathogenesis by promoting adherence of the pathogen to the host intestinal epithelium...
- Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cellsS Gruenheid
Biotechnology Laboratory, University of British Columbia, 6174 University Boulevard, Vancouver V6T 1G3, Canada
Nat Cell Biol 3:856-9. 2001..Cells with null alleles of both mammalian Nck genes are resistant to the effects of EPEC on the actin cytoskeleton. These results implicate Nck adaptors as host-cell determinants of EPEC virulence...
- A brief overview of Escherichia coli O157:H7 and its plasmid O157Ji Youn Lim
Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844, USA
J Microbiol Biotechnol 20:5-14. 2010Enterohemorrhagic Escherichia coli O157:H7 is a major food-borne pathogen causing severe disease in humans worldwide. Healthy cattle are a reservoir of E...
- Rectoanal junction colonization of feedlot cattle by Escherichia coli O157:H7 and its association with supershedders and excretion dynamicsRowland N Cobbold
Field Disease Investigation Unit, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164 6610, USA
Appl Environ Microbiol 73:1563-8. 2007Feedlot cattle were observed for fecal excretion of and rectoanal junction (RAJ) colonization with Escherichia coli O157:H7 to identify potential "supershedders." RAJ colonization and fecal excretion prevalences were correlated, and E...
- Lymphoid follicle-dense mucosa at the terminal rectum is the principal site of colonization of enterohemorrhagic Escherichia coli O157:H7 in the bovine hostStuart W Naylor
Zoonotic and Animal Pathogens Research Laboratory, Medical Microbiology, Edinburgh University, Edinburgh, EH8 9AG, United Kingdom
Infect Immun 71:1505-12. 2003b>Escherichia coli O157:H7 causes bloody diarrhea and potentially fatal systemic sequelae in humans. Cattle are most frequently identified as the primary source of infection, and E...
- Quorum sensing is a global regulatory mechanism in enterohemorrhagic Escherichia coli O157:H7V Sperandio
Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
J Bacteriol 183:5187-97. 2001..These results indicate that quorum sensing is a global regulatory mechanism for basic physiological functions of E. coli as well as for virulence factors...
- Enterohaemorrhagic Escherichia coli O157:H7 target Peyer's patches in humans and cause attaching/effacing lesions in both human and bovine intestineA D Phillips
University Department of Paediatric Gastroenterology, Royal Free Hospital, Pond Street, London NW3 2QG, UK
Gut 47:377-81. 2000..However, although EHEC produce this lesion on cultured human cells, this has not been demonstrated on human intestinal mucosal surfaces. In addition, the initial site(s) of colonisation of EHEC in humans is not known...
- Mauve: multiple alignment of conserved genomic sequence with rearrangementsAaron C E Darling
Department of Computer Science, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Genome Res 14:1394-403. 2004..We have evaluated the quality of Mauve alignments and drawn comparison to other methods through extensive simulations of genome evolution...
- The central Scotland Escherichia coli O157:H7 outbreak: risk factors for the hemolytic uremic syndrome and death among hospitalized patientsS Dundas
Department of Infectious Diseases, Monklands Hospital, Lanarkshire, United Kingdom
Clin Infect Dis 33:923-31. 2001Little is known about risk factors for complications of Escherichia coli O157:H7 infection in adults...
- An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epitheliumArvind Mahajan
Division of Infection and Immunity, The Roslin Institute, Royal Dick School of Veterinary Studies, University of Edinburgh, Penicuik EH25 9EZ, UK
Cell Microbiol 11:121-37. 2009Enterohaemorrhagic Escherichia coli O157 : H7 is a bacterial pathogen that can cause haemorrhagic colitis and haemolytic uremic syndrome. In the primary reservoir host, cattle, the terminal rectum is the principal site of E...
- Escherichia coli O157:H7 survives within human macrophages: global gene expression profile and involvement of the Shiga toxinsKatherine Poirier
Department of Microbiology and Immunology, University of Montreal, C P 6128 Succursale Centre Ville, Montreal, Quebec, Canada H3C 3J7
Infect Immun 76:4814-22. 2008b>Escherichia coli O157:H7 is an important food-borne pathogen that specifically binds to the follicle-associated epithelium in the intestine, which rapidly brings this bacterial pathogen in contact with underlying human macrophages...
- Role for a phage promoter in Shiga toxin 2 expression from a pathogenic Escherichia coli strainP L Wagner
Division of Geographic Medicine and Infectious Diseases, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
J Bacteriol 183:2081-5. 2001Shiga toxins (Stxs), encoded by the stxA and stxB genes, are important contributors to the virulence of Escherichia coli O157:H7 and other Stx-producing E. coli (STEC) strains...
- Prevalence and pathogenicity of Shiga toxin-producing Escherichia coli in beef cattle and their productsH S Hussein
Department of Animal Biotechnology, University of Nevada, Reno 89557, USA
J Anim Sci 85:E63-72. 2007..The results indicated the prevalence of a large number of pathogenic STEC in beef and beef cattle at high rates and emphasized the critical need for control measures to assure beef safety...
- Transcriptional regulators of the GAD acid stress island are carried by effector protein-encoding prophages and indirectly control type III secretion in enterohemorrhagic Escherichia coli O157:H7Jai J Tree
Immunity and Infection Division, The Roslin Institute and R D SVS, University of Edinburgh, Edinburgh EH25 9RG, UK
Mol Microbiol 80:1349-65. 2011..We further demonstrate that PsrA and effectors encoded within cryptic prophage CP933-N are required for persistence in a ruminant model of colonization...
- Analysis of fimbrial gene clusters and their expression in enterohaemorrhagic Escherichia coli O157:H7Alison S Low
Zoonotic and Animal Pathogens Research Laboratory, Centre for Infectious Diseases, Chancellor s Building, University of Edinburgh, Edinburgh, EH16 4SB, UK
Environ Microbiol 8:1033-47. 2006..coli O157:H7 has a limited repertoire of expressed functional fimbriae. This restricted selection of fimbriae may be important in bringing about the tropism E. coli O157:H7 demonstrates for the terminal rectum of cattle...
- Effect of a new probiotic Saccharomyces cerevisiae strain on survival of Escherichia coli O157:H7 in a dynamic gastrointestinal modelLucie Etienne-Mesmin
Clermont Universite, Centre de Recherche en Nutrition Humaine Auvergne, ERT 18, Conception Ingénierie et Développement de l Aliment et du Médicament, BP 10448, F 63000 Clermont Ferrand, France
Appl Environ Microbiol 77:1127-31. 2011Survival of Escherichia coli O157:H7 was investigated using a dynamic gastrointestinal model. A high bacterial mortality was observed in the stomach and duodenum. In contrast, bacteria grew in the distal parts of the small intestine...
- Critical roles for stx2, eae, and tir in enterohemorrhagic Escherichia coli-induced diarrhea and intestinal inflammation in infant rabbitsJennifer M Ritchie
Division of Geographic Medicine and Infectious Diseases, Tufts New England Medical Center, and Howard Hughes Medical Institute, Boston, Massachusetts 02111, USA
Infect Immun 71:7129-39. 2003....
- Human Stx2-specific monoclonal antibodies prevent systemic complications of Escherichia coli O157:H7 infectionJean Mukherjee
Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
Infect Immun 70:612-9. 2002..The Stx2-neutralizing ability of these HuMAbs could potentially be used clinically to passively protect against HUS development in individuals infected with Stx-producing bacteria, including E. coli O157:H7...
- Shiga toxin of enterohemorrhagic Escherichia coli type O157:H7 promotes intestinal colonizationCory M Robinson
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814 4799, USA
Proc Natl Acad Sci U S A 103:9667-72. 2006..Tissue culture experiments showed that this toxin-mediated increase in bacterial adherence correlated with an Stx-evoked increase in a eukaryotic receptor for the EHEC O157:H7 attachment factor intimin...
- Temporal and spatial patterns of bovine Escherichia coli O157 prevalence and comparison of temporal changes in the patterns of phage types associated with bovine shedding and human E. coli O157 cases in Scotland between 1998-2000 and 2002-2004Michael C Pearce
8 Stour Court, Sandwich, Kent, CT13 9FY, UK
BMC Microbiol 9:276. 2009b>Escherichia coli O157 is an important cause of acute diarrhoea, haemorrhagic colitis and, especially in children, haemolytic uraemic syndrome (HUS). Incidence rates for human E...
- Modulation of neutrophil function by a secreted mucinase of Escherichia coli O157:H7Rose L Szabady
Department of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA
PLoS Pathog 5:e1000320. 2009b>Escherichia coli O157:H7 is a human enteric pathogen that causes hemorrhagic colitis which can progress to hemolytic uremic syndrome, a severe kidney disease with immune involvement. During infection, E...
- Preventing household transmission of Shiga toxin-producing Escherichia coli O157 infection: promptly separating siblings might be the keyDirk Werber
Communicable Disease Surveillance Centre, National Public Health Service for Wales, Temple of Peace and Health, Cathays Park, Cardiff, United Kingdom
Clin Infect Dis 46:1189-96. 2008Preventing household transmission of Shiga toxin-producing Escherichia coli O157 (STEC O157) infection is important because of the ease of interpersonal transmission and the potential disease severity.
- The StcE protease contributes to intimate adherence of enterohemorrhagic Escherichia coli O157:H7 to host cellsThomas E Grys
Department of Medical Microbiology and Immunology, University of Wisconsin Madison, 1300 University Ave, Room 481 MSC, Madison, WI 53706, USA
Infect Immun 73:1295-303. 2005..Thus, StcE may help block host clearance of E. coli O157:H7 by destruction of some classes of glycoproteins, and it contributes to intimate adherence of E. coli O157:H7 to the HEp-2 cell surface...
- Characterization of the effects of salicylidene acylhydrazide compounds on type III secretion in Escherichia coli O157:H7Jai J Tree
Zoonotic and Animal Pathogens Research Laboratory, Immunity and Infection Division, The Roslin Institute and Royal Dick School of Veterinary Studies, University of Edinburgh, 49 Little France Crescent, Edinburgh EH16 4SB, United Kingdom
Infect Immun 77:4209-20. 2009..we show that small-molecule inhibitors affect the expression of the type III secretion system (T3SS) of Escherichia coli O157:H7 in liquid culture and when this bacterium is attached to bovine epithelial cells...
- Detection of Salmonella spp., Yersinia enterocolitica and verocytotoxin-producing Escherichia coli O157 in pigs at slaughter in ItalyS Bonardi
Dipartimento di Salute Animale, Sezione di Ispezione degli Alimenti di Origine Animale, Facolta di Medicina Veterinaria, Universita di Parma, Via del Taglio 8, 43100, Parma, Italy
Int J Food Microbiol 85:101-10. 2003..Caecal material and carcass swabs were collected and examined for Salmonella, Yersinia enterocolitica, and Escherichia coli O157. Tonsils were examined for Salmonella and Y. enterocolitica...
- Heterogeneous surface expression of EspA translocon filaments by Escherichia coli O157:H7 is controlled at the posttranscriptional levelAndrew J Roe
Zoonotic and Animal Pathogens Research Laboratory, Medical Microbiology, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, Scotland, UK
Infect Immun 71:5900-9. 2003..Secreted proteins of verotoxigenic Escherichia coli O157 strains include components of a translocation apparatus, EspA, -B, and -D, as well as "effectors"..
- IKKβ phosphorylation regulates RPS3 nuclear translocation and NF-κB function during infection with Escherichia coli strain O157:H7Fengyi Wan
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
Nat Immunol 12:335-43. 2011..Thus, the IKKβ-dependent modification of a specific amino acid in RPS3 promoted specific NF-κB functions that underlie the molecular pathogenetic mechanisms of E. coli O157:H7...
- Organization of the LEE1 operon regulatory region of enterohaemorrhagic Escherichia coli O157:H7 and activation by GrlAMd Shahidul Islam
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Mol Microbiol 79:468-83. 2011..Hence, at the P1 promoter, the action of GrlA resembles that of many MerR family transcription activators at their target promoters...
- Biochemical and structural studies of a HECT-like ubiquitin ligase from Escherichia coli O157:H7David Yin Wei Lin
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
J Biol Chem 286:441-9. 2011..Here, we describe biochemical and structural characterization of an effector protein (NleL) from Escherichia coli O157:H7, a widespread pathogen causing severe foodborne diseases...
- Application of bacteriophages to control intestinal Escherichia coli O157:H7 levels in ruminantsHaiqing Sheng
University of Idaho, Department of Microbiology, Molecular Biology, and Biochemistry, Moscow, ID 83844 3052, USA
Appl Environ Microbiol 72:5359-66. 2006..and a newly isolated phage designated SH1 were tested, alone or in combination, for reducing intestinal Escherichia coli O157:H7 in animals. Oral treatment with phage KH1 did not reduce the intestinal E. coli O157:H7 in sheep...
- Nonpathogenic Escherichia coli can contribute to the production of Shiga toxinShantini D Gamage
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Ohio 45267, USA
Infect Immun 71:3107-15. 2003The food-borne pathogen, Escherichia coli O157:H7, has been associated with gastrointestinal disease and the life-threatening sequela hemolytic uremic syndrome. The genes for the virulence factor, Shiga toxin 2 (Stx2), in E...
- Global effect of RpoS on gene expression in pathogenic Escherichia coli O157:H7 strain EDL933Tao Dong
Department of Biology Life Sciences Building, RM, 433, McMaster University, 1280 Main Street, West Hamilton, ON L8S 4K1, Canada
BMC Genomics 10:349. 2009..The genomic difference may result in substantial changes in RpoS-regulated gene expression. To test this, we compared the transcriptional profile of wild type and rpoS mutants of the E. coli O157:H7 EDL933 type strain...
- Relationships of the Escherichia coli O157, O111, and O55 O-antigen gene clusters with those of Salmonella enterica and Citrobacter freundii, which express identical O antigensGabrielle Samuel
School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
J Bacteriol 186:6536-43. 2004b>Escherichia coli O157, Salmonella enterica O30, and Citrobacter freundii F90 have identical O-antigen structures, as do E. coli O55 and S. enterica O50. The O-antigen gene cluster sequences for E. coli O157 and E...
- Identification and characterization of lpfABCC'DE, a fimbrial operon of enterohemorrhagic Escherichia coli O157:H7Alfredo G Torres
Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
Infect Immun 70:5416-27. 2002The mechanisms underlying the adherence of Escherichia coli O157:H7 and other enterohemorrhagic E. coli (EHEC) strains to intestinal epithelial cells are poorly understood...
- Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary modelPeter C H Feng
Food and Drug Administration, College Park, Maryland 20740, USA
Emerg Infect Dis 13:1701-6. 2007b>Escherichia coli O157:H7 variants were examined for trait mutations and by molecular subtyping to better define clonal complexes postulated on the O157:H7 evolution model...
- Stx-phages: drivers and mediators of the evolution of STEC and STEC-like pathogensHeather E Allison
University of Liverpool, School of Biological Sciences, Division of Integrative Biology, Biosciences Building, Liverpool, Merseyside, UK
Future Microbiol 2:165-74. 2007..toxin, have driven and are driving the emergence of Shiga toxin-producing pathogens such as the infamous Escherichia coli O157:H7...
- Recycling of Shiga toxin 2 genes in sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NMAlexander Mellmann
Institute of Hygiene, University of Munster, Robert Koch Str 41, 48149 Munster, Germany
Appl Environ Microbiol 74:67-72. 2008..The ability to recycle stx(2), a critical virulence trait, makes SF E. coli O157:NM strains ephemeral EHEC that can exist as stx-negative variants during certain phases of their life cycle...
- Distinctiveness of the genomic sequence of Shiga toxin 2-converting phage isolated from Escherichia coli O157:H7 Okayama strain as compared to other Shiga toxin 2-converting phagesToshio Sato
Research Institute, International Medical Center of Japan, 1 21 1, Toyama, Shinjuku ku, Tokyo 162 8655, Japan
Gene 309:35-48. 2003Shiga toxin 2-converting phage was isolated from Escherichia coli O157:H7 associated with an outbreak that occurred in Okayama, Japan in 1996 (M. Watarai, T. Sato, M. Kobayashi, T. Shimizu, S. Yamasaki, T. Tobe, C. Sasakawa and Y...
- Multistate outbreak of Escherichia coli O157:H7 infection associated with consumption of packaged spinach, August-September 2006: the Wisconsin investigationArthur M Wendel
Wisconsin Division of Public Health, Madison, Wisconsin, USA
Clin Infect Dis 48:1079-86. 2009b>Escherichia coli O157:H7 infection often causes hemorrhagic colitis and hemolytic uremic syndrome.
- The S-layer proteins of Lactobacillus crispatus strain ZJ001 is responsible for competitive exclusion against Escherichia coli O157:H7 and Salmonella typhimuriumXueyan Chen
Zhejiang University Institute of Preventive Veterinary Medicine, and Zhejiang Provincial Key Laboratory of Prevent Veterinary Medicine, Hangzhou, PR China
Int J Food Microbiol 115:307-12. 2007..properties such as adhesion, inhibition of the pathogen growth and competitive exclusion against Escherichia coli O157:H7 and Salmonella typhimurium were apparently advantageous over L. acidophilus ATCC 4356...
- In silico genomic analyses reveal three distinct lineages of Escherichia coli O157:H7, one of which is associated with hyper-virulenceChad R Laing
Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, AB, Canada
BMC Genomics 10:287. 2009Many approaches have been used to study the evolution, population structure and genetic diversity of Escherichia coli O157:H7; however, observations made with different genotyping systems are not easily relatable to each other...
- Optical mapping and 454 sequencing of Escherichia coli O157 : H7 isolates linked to the US 2006 spinach-associated outbreakMichael L Kotewicz
Division of Molecular Biology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD 20708, USA
Microbiology 154:3518-28. 2008Optical maps for five representative clinical, food-borne and bovine-derived isolates from the 2006 Escherichia coli O157 : H7 outbreak linked to fresh spinach in the United States showed a common set of 14 distinct chromosomal markers ..
- Iha: a novel Escherichia coli O157:H7 adherence-conferring molecule encoded on a recently acquired chromosomal island of conserved structureP I Tarr
Division of Gastroenterology, Children s Hospital and Regional Medical Center, University of Washington School of Medicine, Seattle, Washington, USA
Infect Immun 68:1400-7. 2000..We conclude that Iha is a novel bacterial adherence-conferring protein and is contained within an E. coli chromosomal island of conserved structure. Pathogenic E. coli O157:H7 has only recently acquired this island...
- Intimin types alpha, beta, and gamma bind to nucleolin with equivalent affinity but lower avidity than to the translocated intimin receptorJames F Sinclair
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
J Biol Chem 279:33751-8. 2004..Taken together, these findings suggest that nucleolin is involved in bacterial adherence promoted by all intimin types and that Tir and nucleolin compete for intimin during adherence...
- Analysis of the expression, regulation and export of NleA-E in Escherichia coli O157 : H7Andrew J Roe
Zoonotic and Animal Pathogens Research Laboratory, Centre for Infectious Disease, The Chancellor s Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK
Microbiology 153:1350-60. 2007..effector proteins such as Tir and Map can be exported via the type III secretion system (T3SS) of Escherichia coli O157 : H7...
- Effect of four probiotic strains and Escherichia coli O157:H7 on tight junction integrity and cyclo-oxygenase expressionHeli Putaala
Danisco Finland, Enteromix Research, Sokeritehtaantie 20, FIN 02460 Kantvik, Finland
Res Microbiol 159:692-8. 2008..acidophilus NCFM, Lactobacillus salivarius Ls-33, and by a cell-free supernatant of a pathogenic bacteria, Escherichia coli O157:H7 (EHEC)...
- Genomic instability in regions adjacent to a highly conserved pch prophage in Escherichia coli O157:H7 generates diversity in expression patterns of the LEE pathogenicity islandZhijie Yang
Department of Food Science and Technology, University of Nebraska, Lincoln, NE 68583 0919, USA
J Bacteriol 191:3553-68. 2009....
- Stomate-based innate immunity against bacterial infection in ArabidopsisMaeli Melotto; Fiscal Year: 2012..Specifically, stomata close in response to Escherichia coli O157:H7 and Pseudomonas syringae pv. tomato (Pst) DC3000 or bacterial PAMPs...
- A New Point of Use Decontamination Method for Fresh ProduceADRIAN J DENVIR; Fiscal Year: 2010..Foodborne illnesses in fruits and vegetables have been linked to several microbial species, including Escherichia coli O157:H7, Salmonella species, Listeria monocytogenes, Shigella species, Cyclospora cayetanensis, Hepatitis A ..
- Delivery of a bacterial inhibitor of NF-kB to colon tumorsPHILIP ROSS HARDWIDGE; Fiscal Year: 2012..This protein, NleH1, expressed by Escherichia coli O157:H7, binds to and inhibits the function of a newly identified subunit of NF-?B, the ribosomal protein S3 (..
- MULTI-ANALYTE IMMUNOCHROMATOGRAPHIC TEST STRIP FOR BACTERIAL TOXIN DETECTIONTHOMAS W OWEN; Fiscal Year: 2011..For example, Escherichia coli O157:H7 infections can spread through contaminated food, contaminated drinking water, contaminated swimming ..
- Protein Translocation by the Type III Secretion SystemPartho Ghosh; Fiscal Year: 2010..Salmonella spp., and Escherichia coli O157:H7 among others...
- Structure of the multidrug efflux protein AcrAPartho Ghosh; Fiscal Year: 2006..RND efflux systems are also encoded by Escherichia coli O157:H7, Salmonella enterica, Shigella flexneri, and Yersinia pestis...
- A High-Performance Food Pathogen Detection InstrumentXiaoli Su; Fiscal Year: 2006..and a low per sample cost for rapid, specific, and sensitive detection of multiple pathogens including Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium in ready-to-eat (RTE) foods, such as processed meat ..
- Electrochemical Method for Viable Pathogen DetectionADRIAN DENVIR; Fiscal Year: 2006..is to develop a new, automated system for the rapid detection of bacterial contamination (especially Escherichia coli O157:H7) in meat and meat products...
- EVOLUTION OF DURABILITY IN EMERGING BACTERIAL PATHOGENSTHOMAS WHITTAM; Fiscal Year: 2000..For Escherichia coli O157:H7, a foodborne pathogen and growing public health problem, the ability to tolerate extreme acidity ..
- NEUROENDOCRINE MEDIATION OF E. COLI 0157:H7 INFECTIONMark Lyte; Fiscal Year: 2003..NE) to increase growth and production of virulence-associated factors of the enteric pathogen Escherichia coli O157:H7. High concentration of NE occur in foods such as ground beef which are contaminated by E. coli O157:H7...
- E. coli O157:H7 genes induced during human infectionINDIRA KUDVA; Fiscal Year: 2005..Here, we propose the identification of Escherichia coli O157:H7 (O157) genes expressed only during human infection, using a recently described novel technique termed ..
- Rapid detection of food borne pathogenic bacteriaSIMON BYSTRYAK; Fiscal Year: 2005..is to develop an ultrasound particle agglutination (UPA) method for determination of pathogenic bacteria Escherichia coli O157:H7 and Listeria monocytogenes in body fluids, food and environmental samples...
- A NEW APPROACH TO MICROBIAL DECONTAMINATION OF FOODThomas Rogers; Fiscal Year: 2001..Significant amounts of pathogens including Escherichia coli O157:H7 and Salmonella typhimurium, cross contaminate beef carcasses throughout processing and can further ..
- INTESTINAL EPITHELIAL RESPONSES TO FOOD BORNE PATHOGENSMartin Kagnoff; Fiscal Year: 2004..of an enteroinvasive foodborne pathogen, Cryptosporidium parvum as a model intraepithelial pathogen, and Escherichia coli O157:H7 as an intraluminal pathogen that lives in intimate association with the apical intestinal epithelial ..
- Screen for Quorum Sensing Molecular Inhibitors (RMI)Vanessa Sperandio; Fiscal Year: 2005....
- EHEC interactions with the normal intestinal floraVanessa Sperandio; Fiscal Year: 2004..In Specific Aim 2, we propose to monitor EHEC virulence gene expression in a mixed population in the presence of the intestinal flora. ..
- Rapid Detection of Major Food-borne PathogensPeixuan Zhu; Fiscal Year: 2003..The instrument will be redesigned to be more compact and portable, and assays developed for use outside a laboratory setting. Tests will be performed on location where these foods are produced, transported and/or prepared. ..
- Development of Vaccine for Neisseria gonorrhoeaePeixuan Zhu; Fiscal Year: 2005..The data from this project will provide fundamental information for understanding the role of this protein in N. gonorrhoeae and the possibility to develop a gonococcal vaccine. ..
- Assay and Instrument Development for Diagnosis of Prion DiseasePeixuan Zhu; Fiscal Year: 2007..Current tests are all postmortem assays using brain tissue. This proposal is to develop a sensitive blood test to detect the disease in its early stage. [unreadable] [unreadable] [unreadable]..
- Ler control of EPEC virulenceJAY MELLIES; Fiscal Year: 2008..Further elucidation of the molecular mechanisms of Ler action should lead to the development of chemotherapeutic agents directed against EPEC and related E. coli O157 bacteria. [unreadable] [unreadable] [unreadable] [unreadable]..
- EVOLUTION OF VIRULENCE BY GENE ACQUISITIONPhillip Tarr; Fiscal Year: 2002..Data generated by this project will elucidate the mechanisms used by virulent organisms to accumulate the necessary genetic components to be successful pathogens. ..
- Chemical Glycobiology on AnthracyclinesPeng Wang; Fiscal Year: 2009..The success of such a platform will accelerate new drug discovery in the field of anticancer drug involving DNA-enzyme-drug complex. This research program should produce new generations of preclinical anthracycline drug candidates. ..
- PATHOPHYSIOLOGY OF CHILDHOOD HEMOLYTIC UREMIC SYNDROMEPhillip Tarr; Fiscal Year: 2005b>Escherichia coli O157:H7-associated hemolytic uremic syndrome (HUS) remains a challenging medical problem. Extensive thrombogenesis, with accompanying fibrinolysis inhibition, precede the earliest indications of renal injury...
- MECHANISMS AND CONTROL OF ACID RESISTANCE IN E COLIJohn Foster; Fiscal Year: 2004..In a broader sense, knowledge gained from this study will also provide insights into fundamental questions concerning proton circulations, protein structure-function relationships and gene expression as each relates to acid stress ..
- SYNTHESES APPLICATIONS OF ALPHA GALACTOSYL EPITOPESPeng Wang; Fiscal Year: 2002....
- MOLECULAR GENETICS OF ENTEROPATHOGENIC E COLI ADHESIONJAMES KAPER; Fiscal Year: 2003..The proposed experimental approach will use a combination of molecular genetics, cell biology, and animal studies to achieve a better understanding of how EPEC infects intestinal epithelial cells and causes disease. ..
- Structure and Function of ADAMTS13 MetalloproteaseX Long Zheng; Fiscal Year: 2009....
- THE GUT MICROBIOME IN DEVELOPMENT, HEALTH, AND DISEASEPhillip Tarr; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- NOVEL E. COLI 0157:H7 INTESTINAL COLONIZATION FACTORSJAMES KAPER; Fiscal Year: 2009DESCRIPTION (provided by applicant): Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is an important cause of hemorrhagic colitis and the hemolytic uremic syndrome (HUS)...
- Dielectrophoretic Separator for Cell/Pathogen SeparationYing Huang; Fiscal Year: 2003..There examples address the needs of lowering the threshold for detection of cancer cells in blood, with the goal of diagnosing infectious disease and biowarfare agents without the need to first culture the pathogens. ..
- Randomonized controlled trial-lactoferrin prevention of diarrhea in childrenTHOMAS GEORGE CLEARY; Fiscal Year: 2010..Diarrhea and malnutrition after weaning is a global problem that may be amenable to this approach. ..
- PEDIATRIC GASTROENTEROLOGY RESEARCH TRAININGPhillip Tarr; Fiscal Year: 2005..D.s with deep commitments to gastrointestinal biology. ..
- DEVELOPMENT OF A LIVE ORAL CHOLERA VACCINEJames B Kaper; Fiscal Year: 2010..Lay summary: Vibrio cholerae is a bacterium that causes cholera, a severe diarrheal disease that can often cause death. This project will develop improved vaccines for the prevention of cholera. ..
- Host and Bacterial Factors in Disease due to H. pyloriKathryn A Eaton; Fiscal Year: 2010..This will allow us to hire additional staff to ensure rapid completion of the remaining experiments. With these changes we fully expect to complete these experiments in 2 years. ..
- PATHOGENIC MECHANISMS AND INTERVENTION STRATEGIES IN HUSRichard Siegler; Fiscal Year: 2002....
- ZAP70 IN T CELL DEVELOPMENTJohn Atkinson; Fiscal Year: 2002..Moreover, these studies may provide additional mechanistic information for therapeutic interventions in drug design for T cell lymphomas, immunodeficiencies, autoimmune diseases, and transplantation rejection. ..
- H PYLORI RECOMBINATION AND GASTRIC COLONIZATIONMartin Blaser; Fiscal Year: 2001..pylori infections. ..
- Type IV Pilins as Vaccines against Bioterrorism ThreatsMichael Donnenberg; Fiscal Year: 2004....
- The eae Gene Cluster of Enteropathogenic E.coliMichael Donnenberg; Fiscal Year: 2007..The significance of this work is that it will lead to a better understanding of how bacteria inject proteins into host cells and interfere with pathways that protect the cells from death and maintain barrier function. ..
- Role of phage in expression & transmission of STX genesDavid Friedman; Fiscal Year: 2008..unreadable] [unreadable]..
- Resistant E.coli in Humans and PoultryJames Johnson; Fiscal Year: 2005..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
- CHEMISTRY AND BIOCHEMISTRY OF NO RELEASING AGENTSPeng Wang; Fiscal Year: 2005..In addition, N-acetylneuraminic acid conjugated RNS will be synthesized as neuraminidase-catalyzed probes to study the interaction of RNS with viruses. ..
- FASEB Conference-Mechanism and Regulation of Prokaryotic TranscriptionCarol Gross; Fiscal Year: 2005..It is also the only meeting where physicists or modelers wishing to enter this field can gain both collaborators and a historical perspective. This activity will have a national impact far beyond the scope of this meeting ..
- Recombinant murine IL-10 produced in situ by H. pyloriKATHRYN EATON; Fiscal Year: 2005..pylori, but will also establish a model in which the roles of other cytokines and mediators can be determined, and host and bacterial interactions can be directly evaluated in vivo. ..
- 2004 Microbial Stress Response Gordon ConferenceCarol Gross; Fiscal Year: 2004..This meeting is generally oversubscribed and given the current program is likely to have very wide appeal. ..
- Development of furanones for treatment of anthraxMartin Blaser; Fiscal Year: 2005..In initial studies, spores will be given subcutaneosly. Efficacy parameters will be defined as above. Successful trials can lead to phase I studies in humans, and treatment studies in non-human primates. ..
- CELL-SURFACE PROTEINS IN CAMPYLOBACTER FETUS VIRULENCEMartin Blaser; Fiscal Year: 2004..Finally, the data generated can be used to develop a mathematical model of antigenic variation that focuses on the relationship between microbial recombination generating diversity and host selection determining the direction of change. ..
- Colloidal Quantum Dot/Well Near-Infrared NanoLabelsYongcheng Liu; Fiscal Year: 2006..3). produce CdS/CdSe/CdTe or CdS/CdTe/CdSe quantum well type-ll nanocrystals by SILAR and develop standard procedures. 4). develop dendron-nanocrystals and box-nanocrystals by chemical modification of these nanocrystals. ..
- RECOMBINOGENIC ENGINEERING OF PATHOGENIC BACTERIAKENAN MURPHY; Fiscal Year: 2003....
- A Nanocrystal Biosensor for Detection of Multiple TBI Neurochemical BiomarkersYongcheng Liu; Fiscal Year: 2007..In comparison to existing technologies such as ELISA, this new technology will be more sensitive, reliable, specific, accurate, portable, robust, inexpensive and rapider to assess TBI. [unreadable] [unreadable] [unreadable]..
- NKT Cells Immunotherapy: Targeting Dendritic Cells with Glycolipid LiposomesPeng Wang; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- 2007 Carbohydrates Gordon Research ConferencePeng Wang; Fiscal Year: 2007..In addition three half-day symposia (three sessions) will be held on the following topics: 1.) carbohydrate structure, 2.) conformational analysis/NMR spectroscopy and 3.) glycoengineering. [unreadable] [unreadable] [unreadable]..
- Nanoarray-Type Detection of Oct3/4 and Cdx2 using AuNPs and E-beam PatternsJeong Yeol Yoon; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- MOLECULAR BASIS OF LOCALIZED ADHERENCE IN E. COLIMichael Donnenberg; Fiscal Year: 2009..The structure of the distantly related B6 variant of bundlin will be solved to shed light on the role of sequence variation in pilus structure and immunogenicity. ..