Genomes and Genes
Summary: A species of fruit fly much used in genetics because of the large size of its chromosomes.
Publications347 found, 100 shown here
- Identification of functional elements and regulatory circuits by Drosophila modENCODESushmita Roy
Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA
Science 330:1787-97. 2010..Our results provide a foundation for directed experimental and computational studies in Drosophila and related species and also a model for systematic data integration toward comprehensive genomic and functional annotation...
- A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3Pablo Cingolani
Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, USA
Fly (Austin) 6:80-92. 2012..SNPs in ~117 Mb unique sequences, representing a substitution rate of ~1/305 nucleotides, between the Drosophila melanogaster w(1118); iso-2; iso-3 strain and the reference y(1); cn(1) bw(1) sp(1) strain...
- The Drosophila melanogaster Genetic Reference PanelTrudy F C Mackay
Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA
Nature 482:173-8. 2012..Here, we describe the Drosophila melanogaster Genetic Reference Panel (DGRP), a community resource for analysis of population genomics and quantitative ..
- The developmental transcriptome of Drosophila melanogasterBrenton R Graveley
Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 6403, USA
Nature 471:473-9. 2011b>Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites...
- FlyBase 101--the basics of navigating FlyBasePeter McQuilton
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
Nucleic Acids Res 40:D706-14. 2012..org) is the leading database and web portal for genetic and genomic information on the fruit fly Drosophila melanogaster and related fly species...
- An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrasesJohannes Bischof
Frontiers in Genetics, National Center of Competence in Research, Institute of Molecular Biology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Proc Natl Acad Sci U S A 104:3312-7. 2007Germ-line transformation via transposable elements is a powerful tool to study gene function in Drosophila melanogaster. However, some inherent characteristics of transposon-mediated transgenesis limit its use for transgene analysis...
- A genome-wide transgenic RNAi library for conditional gene inactivation in DrosophilaGeorg Dietzl
Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, A 1030 Vienna, Austria
Nature 448:151-6. 2007..Here we report the generation and validation of a genome-wide library of Drosophila melanogaster RNAi transgenes, enabling the conditional inactivation of gene function in specific tissues of the intact ..
- MicroRNA targets in DrosophilaAnton J Enright
Computational Biology Center, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
Genome Biol 5:R1. 2003..and characterization of the first few target genes regulated by miRNAs in Caenorhabditis elegans and Drosophila melanogaster have set the stage for elucidation of a novel network of regulatory control...
- The host defense of Drosophila melanogasterBruno Lemaitre
Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
Annu Rev Immunol 25:697-743. 2007To combat infection, the fruit fly Drosophila melanogaster relies on multiple innate defense reactions, many of which are shared with higher organisms...
- Using FlyAtlas to identify better Drosophila melanogaster models of human diseaseVenkateswara R Chintapalli
Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, UK
Nat Genet 39:715-20. 2007..a new online resource, provides the most comprehensive view yet of expression in multiple tissues of Drosophila melanogaster. Meta-analysis of the data shows that a significant fraction of the genome is expressed with great tissue ..
- Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicatorsLin Tian
Howard Hughes Medical Institute, Ashburn, Virginia, USA
Nat Methods 6:875-81. 2009..In Caenorhabditis elegans chemosensory neurons and the Drosophila melanogaster antennal lobe, sensory stimulation-evoked fluorescence responses were significantly enhanced with GCaMP3 (..
- The evolutionary fate and consequences of duplicate genesM Lynch
Department of Biology, University of Oregon, Eugene, OR 97403, USA
Science 290:1151-5. 2000..Although duplicate genes may only rarely evolve new functions, the stochastic silencing of such genes may play a significant role in the passive origin of new species...
- Genome sequence of Aedes aegypti, a major arbovirus vectorVishvanath Nene
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 316:1718-23. 2007..gambiae and Drosophila melanogaster. Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation ..
- A GAL4-driver line resource for Drosophila neurobiologyArnim Jenett
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA
Cell Rep 2:991-1001. 2012We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer...
- Systematic protein location mapping reveals five principal chromatin types in Drosophila cellsGuillaume J Filion
Division of Gene Regulation, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
Cell 143:212-24. 2010..Finally, we provide evidence that the different chromatin types help to target DNA-binding factors to specific genomic regions. These results provide a global view of chromatin diversity and domain organization in a metazoan cell...
- Inhibition of mTOR induces autophagy and reduces toxicity of polyglutamine expansions in fly and mouse models of Huntington diseaseBrinda Ravikumar
Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2XY, UK
Nat Genet 36:585-95. 2004..Our data provide proof-of-principle for the potential of inducing autophagy to treat Huntington disease...
- Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolutionD Allan Drummond
FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA
Cell 134:341-52. 2008....
- bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in DrosophilaJulius Brennecke
European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
Cell 113:25-36. 2003..bantam microRNA simultaneously stimulates cell proliferation and prevents apoptosis. We identify the pro-apoptotic gene hid as a target for regulation by bantam miRNA, providing an explanation for bantam's anti-apoptotic activity...
- Comprehensive analysis of the chromatin landscape in Drosophila melanogasterPeter V Kharchenko
Center for Biomedical Informatics, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 471:480-5. 2011..Here we present a genome-wide chromatin landscape for Drosophila melanogaster based on eighteen histone modifications, summarized by nine prevalent combinatorial patterns...
- Discrete small RNA-generating loci as master regulators of transposon activity in DrosophilaJulius Brennecke
Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, NY 11724, USA
Cell 128:1089-103. 2007..Thus, sense piRNAs, formed following cleavage of transposon mRNAs may enhance production of antisense piRNAs, complementary to active elements, by directing cleavage of transcripts from master control loci...
- The hippo signaling pathway in development and cancerDuojia Pan
Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Dev Cell 19:491-505. 2010..These studies suggest that the core Hippo kinase cascade integrates multiple upstream inputs, enabling dynamic regulation of tissue homeostasis in animal development and physiology...
- A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNAG Hutvagner
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Science 293:834-8. 2001..We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce ..
- P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogasterKoen J T Venken
Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
Science 314:1747-51. 2006We describe a transgenesis platform for Drosophila melanogaster that integrates three recently developed technologies: a conditionally amplifiable bacterial artificial chromosome (BAC), recombineering, and bacteriophage PhiC31-mediated ..
- Transposable elements and the epigenetic regulation of the genomeR Keith Slotkin
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nat Rev Genet 8:272-85. 2007..In addition, new insights have been gained into how this silencing has been co-opted to serve essential functions in 'host' cells, highlighting the importance of TEs in the epigenetic regulation of the genome...
- Identification of novel genes coding for small expressed RNAsM Lagos-Quintana
Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
Science 294:853-8. 2001..This suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated...
- A cis-regulatory map of the Drosophila genomeNicolas Negre
Institute for Genomics and Systems Biology, Department of Human Genetics, The University of Chicago, 900 East 57th Street, Chicago, Illinois 60637, USA
Nature 471:527-31. 2011..Here we describe results from the modENCODE cis-regulatory annotation project. We produced a map of the Drosophila melanogaster regulatory genome on the basis of more than 300 chromatin immunoprecipitation data sets for eight ..
- Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31Amy C Groth
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
Genetics 166:1775-82. 2004..Here we show that this site-specific integration system also functions efficiently in Drosophila melanogaster in cultured cells and in embryos...
- The many landscapes of recombination in Drosophila melanogasterJosep M Comeron
Department of Biology, University of Iowa, Iowa City, Iowa, USA
PLoS Genet 8:e1002905. 2012..We characterized the products of 5,860 female meioses in Drosophila melanogaster by genotyping a total of 139 million informative SNPs and mapped 106,964 recombination events at a ..
- Genomic variation in natural populations of Drosophila melanogasterCharles H Langley
Department of Evolution and Ecology, University of California, Davis, CA 95616, USA
Genetics 192:533-98. 2012This report of independent genome sequences of two natural populations of Drosophila melanogaster (37 from North America and 6 from Africa) provides unique insight into forces shaping genomic polymorphism and divergence...
- Mutations affecting segment number and polarity in DrosophilaC Nusslein-Volhard
Nature 287:795-801. 1980In systematic searches for embryonic lethal mutants of Drosophila melanogaster we have identified 15 loci which when mutated alter the segmental pattern of the larva. These loci probably represent the majority of such genes in Drosophila...
- Evidence for compensatory upregulation of expressed X-linked genes in mammals, Caenorhabditis elegans and Drosophila melanogasterXinxian Deng
Department of Pathology, University of Washington School of Medicine, Seattle, USA
Nat Genet 43:1179-85. 2011..elegans and Drosophila. Our analyses, which take into account the skewed gene content of the X chromosome, support the hypothesis of upregulation of expressed X-linked genes to balance expression of the genome...
- Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivoRichard Benton
Laboratory of Neurogenetics and Behavior, The Rockefeller University, New York, New York, USA
PLoS Biol 4:e20. 2006....
- Adaptive protein evolution at the Adh locus in DrosophilaJ H McDonald
Department of Ecology and Evolutionary Biology, Princeton University, New Jersey 08544
Nature 351:652-4. 1991..DNA sequence data on the Adh locus (encoding alcohol dehydrogenase, EC 18.104.22.168) in three species in the Drosophila melanogaster species subgroup do not fit this expectation; instead, there are more fixed replacement differences ..
- Drosophila pink1 is required for mitochondrial function and interacts genetically with parkinIra E Clark
Nature 441:1162-6. 2006..The role of the pink1-parkin pathway in regulating mitochondrial function underscores the importance of mitochondrial dysfunction as a central mechanism of Parkinson's disease pathogenesis...
- Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channelsDieter Wicher
Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans Knöll St 8, D 07745 Jena, Germany
Nature 452:1007-11. 2008..Thereby, they provide rapid and transient as well as sensitive and prolonged odorant signalling...
- Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genomeAnnette L Parks
Exelixis, 170 Harbor Way, South San Francisco, California 94083 0511, USA
Nat Genet 36:288-92. 2004..The latter methodology enables the generation of small custom deletions with predictable endpoints throughout the genome and should make their isolation a simple and routine task...
- Coding of odors by a receptor repertoireElissa A Hallem
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
Cell 125:143-60. 2006..The space provides a basis for predicting behavioral responses to odors...
- A protein complex network of Drosophila melanogasterK G Guruharsha
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Cell 147:690-703. 2011..The DPiM represents, to our knowledge, the largest metazoan protein complex map and provides a valuable resource for analysis of protein complex evolution...
- HDAC6 rescues neurodegeneration and provides an essential link between autophagy and the UPSUdai Bhan Pandey
Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
Nature 447:859-63. 2007..Here we show that autophagy acts as a compensatory degradation system when the UPS is impaired in Drosophila melanogaster, and that histone deacetylase 6 (HDAC6), a microtubule-associated deacetylase that interacts with ..
- Role of histone H2A ubiquitination in Polycomb silencingHengbin Wang
Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
Nature 431:873-8. 2004..Thus, our studies identify the H2A ubiquitin ligase, and link H2A ubiquitination to Polycomb silencing...
- Drosophila parkin requires PINK1 for mitochondrial translocation and ubiquitinates mitofusinElena Ziviani
Department of Biomedical Sciences, Medical Research Council Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN, United Kingdom
Proc Natl Acad Sci U S A 107:5018-23. 2010..We hypothesize that Mfn ubiquitination may provide a mechanism by which terminally damaged mitochondria are labeled and sequestered for degradation by autophagy...
- Sequencing of pooled DNA samples (Pool-Seq) uncovers complex dynamics of transposable element insertions in Drosophila melanogasterRobert Kofler
Institut für Populationsgenetik, Vetmeduni Vienna, Wien, Austria
PLoS Genet 8:e1002487. 2012..We apply this method to data from a natural Drosophila melanogaster population from Portugal...
- A genome-scale shRNA resource for transgenic RNAi in DrosophilaJian Quan Ni
Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts, USA
Nat Methods 8:405-7. 2011Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system ..
- Asymmetry in the assembly of the RNAi enzyme complexDianne S Schwarz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, MA 01605, USA
Cell 115:199-208. 2003..Thus, the common step of RISC assembly is an unexpected source of asymmetry for both siRNA function and miRNA biogenesis...
- BioGRID: a general repository for interaction datasetsChris Stark
Ontario Cancer Institute, Princess Margaret Hospital, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
Nucleic Acids Res 34:D535-9. 2006..0 includes >116 000 interactions from Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster and Homo sapiens...
- Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAsQingfa Wu
Department of Plant Pathology and Microbiology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA
Proc Natl Acad Sci U S A 107:1606-11. 2010..We also report detection of virus-derived PIWI-interacting RNAs (piRNAs) in Drosophila melanogaster that have not been previously described in any other host species and demonstrate viral genome assembly ..
- Mechanisms of life span extension by rapamycin in the fruit fly Drosophila melanogasterIvana Bjedov
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, UK
Cell Metab 11:35-46. 2010..Rapamycin could increase life span of weak insulin/Igf signaling (IIS) pathway mutants and of flies with life span maximized by dietary restriction, indicating additional mechanisms...
- Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfactionMattias C Larsson
Laboratory of Neurogenetics and Behavior, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Neuron 43:703-14. 2004..Consistent with this cellular defect, the Or83b mutation disrupts behavioral and electrophysiological responses to many odorants. Or83b therefore encodes an atypical odorant receptor that plays an essential general role in olfaction...
- TRANSFAC and its module TRANSCompel: transcriptional gene regulation in eukaryotesV Matys
BIOBASE GmbH, Halchtersche Strasse 33, D 38304 Wolfenbuttel, Germany
Nucleic Acids Res 34:D108-10. 2006..The here described public releases, TRANSFAC 7.0 and TRANSCompel 7.0, are accessible under http://www.gene-regulation.com/pub/databases.html...
- Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAsJ Graham Ruby
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Genome Res 17:1850-64. 2007..to predict miRNAs conserved among Drosophila species and large-scale sequencing of small RNAs from Drosophila melanogaster to experimentally confirm and complement these predictions...
- RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryoJulia Zeitlinger
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
Nat Genet 39:1512-6. 2007..We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development...
- The BioGRID Interaction Database: 2008 updateBobby Joe Breitkreutz
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
Nucleic Acids Res 36:D637-40. 2008..Source code for the BioGRID and the linked Osprey network visualization system is now freely available without restriction...
- An internal thermal sensor controlling temperature preference in DrosophilaFumika N Hamada
National Center for Behavioral Genomics, Volen Center for Complex Systems, Biology Department, Brandeis University MS 008, 415 South Street, Waltham, Massachusetts 02454, USA
Nature 454:217-20. 2008..This provides a potentially general strategy for robustly selecting a narrow temperature range optimal for survival...
- Wolbachia and virus protection in insectsLauren M Hedges
School of Integrative Biology, University of Queensland, Brisbane 4072, Australia
Science 322:702. 2008..We demonstrate a fitness benefit for Wolbachia-infected insects that may explain this paradox. Drosophila melanogaster flies infected with Wolbachia are less susceptible to mortality induced by a range of RNA viruses...
- Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenesMichele Markstein
Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Genet 40:476-83. 2008..The generality of our approach makes it adaptable to other model systems to identify and modify loci for optimal transgene expression...
- Application of a time-delay neural network to promoter annotation in the Drosophila melanogaster genomeM G Reese
Berkeley Drosophila Genome Project, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
Comput Chem 26:51-6. 2001..of a eukaryotic core promoter region has been developed and its application for analysis of the Drosophila melanogaster genome is presented...
- Correlates of sleep and waking in Drosophila melanogasterP J Shaw
The Neurosciences Institute, 10640 John Jay Hopkins Drive, San Diego, CA 92121, USA
Science 287:1834-7. 2000..These results implicate the catabolism of monoamines in the regulation of sleep and waking in the fly and suggest that Drosophila may serve as a model system for the genetic dissection of sleep...
- Deep annotation of Drosophila melanogaster microRNAs yields insights into their processing, modification, and emergenceEugene Berezikov
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands
Genome Res 21:203-15. 2011..We report here a meta-analysis of short RNA data from Drosophila melanogaster, aggregating published libraries with 76 data sets that we generated for the modENCODE project...
- The generic genome browser: a building block for a model organism system databaseLincoln D Stein
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11790, USA
Genome Res 12:1599-610. 2002..GBrowse is freely available under an open source license. The software, its documentation, and support are available at http://www.gmod.org...
- G protein-coupled receptors in Anopheles gambiaeCatherine A Hill
Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
Science 298:176-8. 2002..expression and, along with 76 putative gustatory receptors, for their molecular evolution relative to Drosophila melanogaster. Examples of lineage-specific gene expansions were observed as well as a single instance of unusually ..
- Layered reward signalling through octopamine and dopamine in DrosophilaChristopher J Burke
Department of Neurobiology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
Nature 492:433-7. 2012..In addition, they reconcile previous findings with octopamine and dopamine and suggest that reinforcement systems in flies are more similar to mammals than previously thought...
- Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9Xingjie Ren
Gene Regulatory Laboratory, School of Medicine, Tsinghua University, Beijing 100084, China
Proc Natl Acad Sci U S A 110:19012-7. 2013..Here we report an effective and inexpensive method for genome DNA editing in Drosophila melanogaster whereby plasmid DNAs encoding short sgRNAs under the control of the U6b promoter are injected into ..
- Accessibility of the Drosophila genome discriminates PcG repression, H4K16 acetylation and replication timingOliver Bell
Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
Nat Struct Mol Biol 17:894-900. 2010..Here, we measured genome-wide DNA accessibility in Drosophila melanogaster by combining M.SssI methylation footprinting with methylated DNA immunoprecipitation...
- hippo encodes a Ste-20 family protein kinase that restricts cell proliferation and promotes apoptosis in conjunction with salvador and wartsShian Wu
Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Cell 114:445-56. 2003..A human homolog of hpo completely rescues the overgrowth phenotype of Drosophila hpo mutants, suggesting that hpo might play a conserved role for growth control in mammals...
- A microRNA in a multiple-turnover RNAi enzyme complexGyorgy Hutvagner
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
Science 297:2056-60. 2002..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
- Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogasterFleur Ponton
School of Biological Sciences, Heydon Laurence Building A08, The University of Sydney, NSW 2006, Australia
J Insect Physiol 57:840-50. 2011b>Drosophila melanogaster is one of the most important genetic models and techniques such as reverse transcription quantitative real-time PCR (RT-qPCR) are being employed extensively for deciphering the genetics basis of physiological ..
- Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovaryColin D Malone
Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
Cell 137:522-35. 2009....
- X chromosome dosage compensation via enhanced transcriptional elongation in DrosophilaErica Larschan
Division of Genetics, Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
Nature 471:115-8. 2011..Improving transcriptional output downstream of typical gene-specific controls may explain how dosage compensation can be imposed on the diverse set of genes along an entire chromosome...
- Evolution of supergene families associated with insecticide resistanceHilary Ranson
Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
Science 298:179-81. 2002..A comparative genomic analysis with Drosophila melanogaster reveals that a considerable expansion of these supergene families has occurred in the mosquito...
- The Toll and Imd pathways are the major regulators of the immune response in DrosophilaEnnio De Gregorio
Centre de Genetique Moleculaire, CNRS, F 91198 Gif sur Yvette, France
EMBO J 21:2568-79. 2002..Interestingly, we observed that some Drosophila immune-responsive genes are located in gene clusters, which often are transcriptionally co-regulated...
- RNA polymerase is poised for activation across the genomeGinger W Muse
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
Nat Genet 39:1507-11. 2007..importance of polymerase stalling for transcription regulation, we carried out a genome-wide search for Drosophila melanogaster genes with Pol II stalled within the promoter-proximal region...
- GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targetsJoerg E Braun
Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
Mol Cell 44:120-33. 2011....
- Combinatorial binding predicts spatio-temporal cis-regulatory activityRobert P Zinzen
European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
Nature 462:65-70. 2009..This data-driven approach does not require previous knowledge of transcription factor sequence affinity, function or expression, making it widely applicable...
- Polycomb-dependent regulatory contacts between distant Hox loci in DrosophilaFrédéric Bantignies
Institut de Genetique Humaine, CNRS UPR 1142, Montpellier, France
Cell 144:214-26. 2011In Drosophila melanogaster, Hox genes are organized in an anterior and a posterior cluster, called Antennapedia complex and bithorax complex, located on the same chromosome arm and separated by 10 Mb of DNA...
- An endogenous small interfering RNA pathway in DrosophilaBenjamin Czech
Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nature 453:798-802. 2008..These observations expand the repertoire of small RNAs in Drosophila, adding a class that blurs distinctions based on known biogenesis mechanisms and functional roles...
- A high-sugar diet produces obesity and insulin resistance in wild-type DrosophilaLaura Palanker Musselman
Division of Endocrinology, Metabolism, and Lipid Research, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
Dis Model Mech 4:842-9. 2011..To gain insights into how dietary excess affects insulin resistance, we studied the simple model organism Drosophila melanogaster. Larvae reared on a high-sugar diet were hyperglycemic, insulin resistant and accumulated fat--hallmarks ..
- The regulatory activity of microRNA* species has substantial influence on microRNA and 3' UTR evolutionKatsutomo Okamura
Sloan Kettering Institute, Department of Developmental Biology, 521 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, New York 10065, USA
Nat Struct Mol Biol 15:354-63. 2008..Here we show that, although Drosophila melanogaster miRNA* species are less abundant than their partners, they are often present at physiologically relevant ..
- Remote control of insulin secretion by fat cells in DrosophilaCharles Geminard
Institute for Developmental Biology and Cancer, CNRS University of Nice Sophia Antipolis, Parc Valrose, 06108 Nice, France
Cell Metab 10:199-207. 2009..Thus, the availability of nutrients is remotely sensed in fat body cells and conveyed to the brain IPCs by a humoral signal controlling ILP release...
- Drosophila OBP LUSH is required for activity of pheromone-sensitive neuronsPingxi Xu
Department of Pharmacology, Center for Basic Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75335, USA
Neuron 45:193-200. 2005..These studies directly link pheromone-induced behavior with OBP-dependent activation of a subset of olfactory neurons...
- Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and functionEric Lecuyer
Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada
Cell 131:174-87. 2007..A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions...
- Insect olfactory receptors are heteromeric ligand-gated ion channelsKoji Sato
Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
Nature 452:1002-6. 2008..The ligand-gated ion channel formed by an insect OR complex seems to be the basis for a unique strategy that insects have acquired to respond to the olfactory environment...
- Sirtuin 2 inhibitors rescue alpha-synuclein-mediated toxicity in models of Parkinson's diseaseTiago Fleming Outeiro
Alzheimer s Research Unit, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129, USA
Science 317:516-9. 2007..Furthermore, the inhibitors protected against dopaminergic cell death both in vitro and in a Drosophila model of Parkinson's disease. The results suggest a link between neurodegeneration and aging...
- Systems genetics of complex traits in Drosophila melanogasterJulien F Ayroles
Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA
Nat Genet 41:299-307. 2009..Overlapping transcripts in modules associated with different traits provide insight into the molecular basis of pleiotropy between complex traits...
- The endocrine regulation of aging by insulin-like signalsMarc Tatar
Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, USA
Science 299:1346-51. 2003....
- Rest in Drosophila is a sleep-like stateJ C Hendricks
Center for Sleep and Respiratory Neurobiology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
Neuron 25:129-38. 2000To facilitate the genetic study of sleep, we documented that rest behavior in Drosophila melanogaster is a sleep-like state...
- A microRNA imparts robustness against environmental fluctuation during developmentXin Li
Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, Illinois 60208, USA
Cell 137:273-82. 2009..We suggest that some conserved microRNAs like miR-7 may enter into novel genetic relationships to buffer developmental programs against variation and impart robustness to diverse regulatory networks...
- Improved splice site detection in GenieM G Reese
Human Genome Informatics Group, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
J Comput Biol 4:311-23. 1997..In further splice site experiments, we also looked at correlations between splice site scores and intron and exon lengths, as well as at the effect of distance to the nearest splice site on false positive rates...
- The shaping of male courtship posture by lateralized gustatory inputs to male-specific interneuronsMasayuki Koganezawa
Division of Neurogenetics, Tohoku University Graduate School of Life Sciences, Sendai 980 8578, Japan
Curr Biol 20:1-8. 2010Unilateral wing vibration to generate "love songs" is a hallmark of male courtship posture in Drosophila melanogaster. In contrast, males of some other Drosophila species extend both wings simultaneously during courtship...
- Genome-wide analysis of a long-term evolution experiment with DrosophilaMolly K Burke
Ecology and Evolutionary Biology, University of California, Irvine, 321 Steinhaus Hall, Irvine, California 92697 2525, USA
Nature 467:587-90. 2010..Here we present whole-genome resequencing data from Drosophila melanogaster populations that have experienced over 600 generations of laboratory selection for accelerated ..
- Regulation of Drosophila lifespan by JNK signalingBenoit Biteau
Department of Biology, University of Rochester, Rochester, NY 14627, USA
Exp Gerontol 46:349-54. 2011..Here, we review recent studies in Drosophila that elucidate the tissue-specific and systemic consequences of JNK activation that ultimately impact lifespan of the organism...
- Functional genomic screen reveals genes involved in lipid-droplet formation and utilizationYi Guo
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA
Nature 453:657-61. 2008..These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage...
- Analysis of homeodomain specificities allows the family-wide prediction of preferred recognition sitesMarcus B Noyes
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 133:1277-89. 2008..This resource also allows prediction of homeodomain specificities from other organisms, which is demonstrated by the prediction and analysis of human homeodomain specificities...
- Developmental roles of 21 Drosophila transcription factors are determined by quantitative differences in binding to an overlapping set of thousands of genomic regionsStewart MacArthur
Genomics Division, Lawrence Berkeley National Laboratory, Cyclotron Road MS 84 181, Berkeley, CA 94720, USA
Genome Biol 10:R80. 2009....
- Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cellsMegha Ghildiyal
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 320:1077-81. 2008..in length, that correspond to transposons and heterochromatic sequences in the somatic cells of Drosophila melanogaster. We also detected endo-siRNAs complementary to messenger RNAs (mRNAs); these siRNAs disproportionately ..
- Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complexD D Sarbassov
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
Science 307:1098-101. 2005..Rictor-mTOR may serve as a drug target in tumors that have lost the expression of PTEN, a tumor suppressor that opposes Akt/PKB activation...
- The Drosophila melanogaster transcriptome by paired-end RNA sequencingBryce Daines
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Genome Res 21:315-24. 2011RNA-seq was used to generate an extensive map of the Drosophila melanogaster transcriptome by broad sampling of 10 developmental stages. In total, 142...
- Expression pattern and, surprisingly, gene length shape codon usage in Caenorhabditis, Drosophila, and ArabidopsisL Duret
Laboratoire de Biometrie, Génétique et Biologie des Populations, Unité Mixte de Recherche Centre National de la Recherche Scientifique 5558, Villeurbanne Cedex, France
Proc Natl Acad Sci U S A 96:4482-7. 1999..and analyzed codon usage in 8,133, 1,550, and 2,917 genes, respectively, from Caenorhabditis elegans, Drosophila melanogaster, and Arabidopsis thaliana...
- GAL4 system in Drosophila: a fly geneticist's Swiss army knifeJoseph B Duffy
Department of Biology, Indiana University, 1001 E 3rd Street, Bloomington, IN 47405, USA
Genesis 34:1-15. 2002
- Activation of pheromone-sensitive neurons is mediated by conformational activation of pheromone-binding proteinJohn D Laughlin
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, 12801 East 17th Avenue, M S 8303, P O Box 6511, Aurora, CO 80045, USA
Cell 133:1255-65. 2008..Therefore, the pheromone-binding protein is an inactive, extracellular ligand converted by pheromone molecules into an activator of pheromone-sensitive neurons and reveals a distinct paradigm for detection of odorants...
- A single p450 allele associated with insecticide resistance in DrosophilaP J Daborn
Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
Science 297:2253-6. 2002..Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a ..
- Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1Janghoo Lim
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Nature 452:713-8. 2008..This model provides mechanistic insight into the molecular pathogenesis of SCA1 as well as other polyglutamine diseases...
- Sphingolipid Metabolism in Drosophila DevelopmentJulie D Saba; Fiscal Year: 2013..Studies supported by this grant have explored the role of sphingolipids in the model organism Drosophila melanogaster. These studies have resulted in the chemical characterization of Drosophila sphingoid bases and the ..
- Using Drosophila to understand WNK and SPAK/OSR1 regulation of SLC12 cotransporteAYLIN RACHEL RODAN; Fiscal Year: 2013..D., and fellowship studies, and in particular previous experience studying Drosophila melanogaster, by developing new scientific skills in physiology and biochemistry...
- Novel genomic effects of Y-linked polymorphismsDaniel L Hartl; Fiscal Year: 2012..The novelty emerges from our recent discovery that the Y chromosome of Drosophila melanogaster is polymorphic for sequences that differentially affect the expression of many hundreds of autosomal and ..
- Persisting Grand Mal and Myoclonic Seizures in JME including VeteransAntonio V Delgado-Escueta; Fiscal Year: 2013..guide which putative epilepsy genes have priority for studies of functions and disease mechanisms in Drosophila melanogaster (Aim 3), mice (Aim 2) and humans (Aim 1)...
- Regulation of Rhox5 Transcriptional ElongationMarcy E Richardson; Fiscal Year: 2013..Recent studies conducted in human embryonic stem cells and Drosophila melanogaster embryos have identified transcriptional elongation as a widely used alternative control mechanism of ..
- Analysis of the functions of axon guidance genes during vector mosquito developmeMOLLY DUMAN SCHEEL; Fiscal Year: 2012..recent years, evolutionary developmental biologists have applied knowledge of developmental genetics in Drosophila melanogaster, a genetically tractable model organism, to better understand development of other arthropods...
- Oxidative stress and neurogenetic networks in DrosophilaRobert R H Anholt; Fiscal Year: 2010..b>Drosophila melanogaster provides a genetically tractable model system, because the genetic background and environmental ..
- Genetic Control of Axial Patterning in Drosophila EyeAmit Singh; Fiscal Year: 2010..A well established model of the Drosophila melanogaster (fruit fly) will be employed to study dorso-ventral (DV) (axial) patterning and growth in the developing ..
- AAA ATPases linking mitochondria with microtubule processing in flies and yeastKaren G Hales; Fiscal Year: 2012..Many human genetic neurodegenerative disorders are associated with defects of mitochondrial dynamics. Drosophila melanogaster spermatogenesis is an ideal model system for genetic dissection of conserved mechanisms of mitochondrial ..
- Molecular Genetics of Caloric Restriction in Aging FliesBlanka Rogina; Fiscal Year: 2013..Mutation in the Indy gene in the fruit fly, Drosophila melanogaster, dramatically extends life span...
- Is symmetrical dimethylation of Arginine 2 of Histone H3 used as an epigenetic siMarjorie A Oettinger; Fiscal Year: 2010..Fourth, we will analyze these modifications in model organisms (S. cerevisiae, S. pombe and Drosophila melanogaster), determining both the genome-wide localization pattern of the modifications and whether they are ..
- Analyzing Anthrax Toxins in DrosophiliaEthan Bier; Fiscal Year: 2013..in barrier disruption We discovered this novel effect of anthrax toxins using the model genetic system Drosophila melanogaster (fruit fly) and then validated these effects in human vascular endothelial cells as well as in vivo in ..
- Genome-wide Identification of Immediate Early Genes in InsectsAnandasankar Ray; Fiscal Year: 2012..few genes have been identified as IEGs in mammals, but very little is known about IEGs in insects such as Drosophila melanogaster, which is used as a model to study these biological process...
- EGF RECEPTOR MEDIATED SIGNALING IN DROSOPHILAGERTRUD M SCHUPBACH; Fiscal Year: 2013..focuses on signaling between the female germline and its surrounding follicle cells in the ovary of Drosophila melanogaster. We have shown that the Drosophila Egfr is expressed in the follicle cells and receives a highly ..
- Insulin, IGF and Insulin Signaling: effects on Anopheles lifespan and immunityMICHAEL ALLEN RIEHLE; Fiscal Year: 2012..impacts on mosquito physiology and vector competence that were conceptualized for Caenorhabditis elegans, Drosophila melanogaster and mammals...
- Regulation of TRP channels and visual transductionCraig Montell; Fiscal Year: 2013..goal of our research is to understand the mechanisms underlying phototransduction in the fruitfly, Drosophila melanogaster. Drosophila phototransduction functions through a phospholipase C (PLC)- dependent signaling system, and ..
- Expression control in Drosophila splicing assembly factor RNP-4FJACK VAUGHN; Fiscal Year: 2010..of the evolutionarily-conserved splicing assembly factor RNP-4F in the important genetic model organism Drosophila melanogaster. Specifically, we postulate that an isoform of dADAR adenosine deaminase plays a major role in a ..
- Genetic and Molecular Studies of Drosophila Chromatin Remodeling FactorsJohn W Tamkun; Fiscal Year: 2013..To address these important issues, our laboratory uses Drosophila melanogaster as a model organism to study the function of chromatin-remodeling and modifying enzymes...
- How do Amine Neurons Work?Edward A Kravitz; Fiscal Year: 2013..These experimental approaches are the farthest advanced in fruit flies (Drosophila melanogaster) where powerful genetic methods allow identification and manipulation of single neurons concerned with ..
- Using Drosophila as a model to understand TDP-43 function in ALSDavid B Morton; Fiscal Year: 2012..We are using the fruit fly, Drosophila melanogaster, as a model to understand the function of TDP- 43 in neurons and the consequences of aberrant expression...
- Genetic control of quantitative traits associated with the metabolic syndromeMaria De Luca; Fiscal Year: 2013..These genetic pathways will be identified by integrating genetic and expression network analysis in Drosophila melanogaster. Using this information, human population-based association studies will be performed to investigate the ..
- Generation of Wunen/LPP3-based therapy for muscular dystrophyHannele Ruohola Baker; Fiscal Year: 2010..of LPP3 that reduces the level of active second messenger, S1P can suppress dystrophic phenotypes in Drosophila melanogaster. We will now study the mechanism of sphingolipid pathway and Dystrophin interactions and further, will ..
- Regulation of tubulogenesis in the Drosophila ovaryCeleste A Berg; Fiscal Year: 2012..This proposal uses Drosophila melanogaster egg chambers to investigate the mechanisms that control tube size and shape...
- Genetic and Molecular Mechanisms of Ethanol-Induced Developmental DefectsRACHAEL LOUISE FRENCH; Fiscal Year: 2013..and study the molecular targets of developmental ethanol using the genetically amenable model organism Drosophila melanogaster. Drosophila melanogaster, the common fruit fly, has been utilized extensively in biological research, ..
- Proteomics of Cell Death via 2-D Microfluidic ProfilingDON DEVOE; Fiscal Year: 2009..By using the fruit fly Drosophila melanogaster as a model system, these studies will explore pathways and identify biomarkers associated with Caspase ..
- A Drosophila Model for Genetic Studies of MetabolismCarl S Thummel; Fiscal Year: 2010..The goal of our research is to use the fruit fly, Drosophila melanogaster, as a simple model system to define the central regulatory pathways that control metabolism and maintain ..
- Identifying novel anti-infectives by high through-put screening in whole animalsFrederick M Ausubel; Fiscal Year: 2013..in 384-well plates is followed by secondary screening in a more highly evolved model host, the fruit fly Drosophila melanogaster, increasing the likelihood of isolating drugs that will work in humans...
- Group II Intron Mobility and Gene TargetingAlan M Lambowitz; Fiscal Year: 2012..group II intron-based gene targeting methods for eukaryotic model organisms, focusing initially on Drosophila melanogaster. (4) To use Xenopus tropicalis to further develop methods for using group II introns for gene targeting ..
- Discovery of Vibrio cholerae factors contributing to virulence in Drosophila.ALEXANDRA ELIZABETH PURDY; Fiscal Year: 2010..Previous work in the Watnick laboratory has established Drosophila melanogaster as a model organism for V. cholerae intestinal infection...
- Steroid-Dependent Changes in the Yorkie InteractomeAlexey Veraksa; Fiscal Year: 2013..SWH) pathway is a well-conserved metazoan signaling cascade that restricts organ size in the fruit fly Drosophila melanogaster and limits tumorigenesis in mammals...
- Aging specific gene expression in DrosophilaJOHN GERARD TOWER; Fiscal Year: 2013..of this research is to understand the regulation of gene expression during aging using the model organism Drosophila melanogaster. Aging in both flies and humans is found to be associated with a tissue-specific induction of oxidative ..
- Octopamine Neuroanatomy and Functioning in Fruit Fly Social BehaviorKYLE LYNN GOBROGGE; Fiscal Year: 2013..The fruit fly (Drosophila melanogaster) genome is fully sequenced, and as a model system, fruit flies offer numerous possibilities for ..
- Developmental Regulation of the Cell Cycle in DrosophilaLAURA ANNE LEE; Fiscal Year: 2013..conservation of genes involved in the cell cycle combined with the superb genetics and cytology of Drosophila melanogaster make it an ideal model organism for studying cell-cycle regulation in a developmental context...
- Drosophila feeding behavior: New tools for the genetic dissection of prandiologyWilliam W Ja; Fiscal Year: 2012..The fruit fly, Drosophila melanogaster, is a powerful system for studying the relationship of genes with behavior and disease...
- Hormonal Modulation of Aggression in DrosophilaEdward A Kravitz; Fiscal Year: 2013..Over the past decade, we have developed a Drosophila melanogaster model of aggression...
- Nuclear mechanics and mechanotransduction in muscular laminopathiesJan Lammerding; Fiscal Year: 2013..Using drosophila melanogaster models expressing a panel of lamin mutations with variable muscle involvement, we will relate effects of ..
- Interplay between the Endocrine and Innate Systems of DrosphilaNeal Silverman; Fiscal Year: 2013..This proposal uses the fruit fly Drosophila melanogaster as a model for the study of these interactions...
- A model of kidney stone disease using D. melanogasterMarshall Stoller; Fiscal Year: 2013..In Drosophila melanogaster (D...
- Regulation of RNA SurveillanceMILES FROME WILKINSON; Fiscal Year: 2012..analyses from several groups indicate that between 5 and 10% of the genes in Saccharomyces cerevisiae, Drosophila melanogaster, and mammals give rise to transcripts that are subject to NMD...
- Developing cell lines from clock neurons in DrosophilaPaul E Hardin; Fiscal Year: 2013DESCRIPTION (provided by applicant): The identification and analysis of clock genes in the fruit fly, Drosophila melanogaster, revealed that the circadian clock is based on autoregulatory feedback loops in gene expression...
- Taste and feeding circuits in DrosophilaMICHAEL DOUGLAS GORDON; Fiscal Year: 2010..proposal will examine the neural substrates of taste perception and feeding behavior in the fruit fly Drosophila melanogaster. Flies offer an excellent model system for studying this problem because they exhibit robust behavioral ..
- Function of the Six and Eya Genes in Retinal DevelopmentJustin P Kumar; Fiscal Year: 2012..patients and mouse model systems as well as total elimination of the compound eyes of the fruit fly, Drosophila melanogaster. The association of Six and Eya gene lesions with retinal disorders in both insect and mammalian systems ..
- Role of Myc in the Growth Regulation PathwaysPAOLA BELLOSTA BELLOSTA; Fiscal Year: 2010..In our laboratory we are using the fruitfly Drosophila melanogaster as an animal model to study the processes that regulate these diseases...
- Small RNA and whole chromosome recognition in Drosophila melanogaster.Victoria H Meller; Fiscal Year: 2013..The similarities between analogous processes in flies and mammals suggest that this mechanism may also contribute to normal genome regulation in humans. ..
- Mobilization of Triacylglycerol Stores in InsectsEstela L Arrese; Fiscal Year: 2012..The main lipase found in the insect fat body, TG-lipase (TGL) homolog to Drosophila melanogaster CG8552, and a lipid droplet-associated protein, Lsd1, whose phosphorylation stimulates the rate of ..
- The epigenetic basis of cytoplasmic incompatibilityKRISTIN KALIE JERNIGAN; Fiscal Year: 2012..iii) Third, our preliminary evidence shows that infected Drosophila melanogaster flies treated with a small molecule inhibitor of DNA methylation (5-Azacytidine) exhibit CI when only one ..
- Genetics and Molecular Biology of Striated Muscle MyosinSanford I Bernstein; Fiscal Year: 2013..will employ a multidisciplinary approach that takes advantage of the powerful genetic tools available for Drosophila melanogaster along with a broad range of expertise that allows us to study myosin in an integrative manner, from its ..
- Ecdysone modulation of sexual behavior in Drosophila melanogasterGEOFFREY GANTER; Fiscal Year: 2009We will explore the control of sexual behavior of the fruit fly by the steroid hormone ecdysone. The Drosophila melanogaster model system has produced a quantity of data relevant to higher functions of the human nervous system, ..
- Cellular Biomechanics of Heat-Shock Induced Defects in Drosophila EmbryogenesisM Shane Hutson; Fiscal Year: 2013..The project focuses on similar effects in the model system of Drosophila melanogaster in which the developmental defects have been termed phenocopies for their ability to mimic the phenotypes ..
- IDENTIFICATION OF GENES ASSOCIATED WITH MYOBLAST FUSIONSusan M Abmayr; Fiscal Year: 2012..We have chosen embryonic development of the larval body wall muscles in the fruitfly Drosophila melanogaster as a model system, in an effort to utilize the advantages of a combined genetic and molecular approach...
- Neuronal Cell Cycle and SurvivalNicholas E Baker; Fiscal Year: 2013..The eye of the fruit fly Drosophila melanogaster will be used to evaluate this new hypothesis, and to identify specific aspects of cell cycle progression, ..
- A Drosophila Model for Williams Syndrome Cognitive ProcessingRalph J Greenspan; Fiscal Year: 2010..This proposal outlines a plan to develop a behavioral/genetic model in Drosophila melanogaster to study the distinctive cognitive phenotype affecting visuospatial processing found in humans with ..
- Function of the Six and Eya Genes in Retinal DevelopmentJUSTIN KUMAR; Fiscal Year: 2009..patients and mouse model systems as well as total elimination of the compound eyes of the fruit fly, Drosophila melanogaster. The association of Six and Eya gene lesions with retinal disorders in both insect and mammalian systems ..
- Variable Dose Rate X-ray IrradiatorHoward B Lieberman; Fiscal Year: 2010..ideal to enable studies to be undertaken on a wide array of model systems such as radioresistant yeast, Drosophila melanogaster and Caenorhabditis elegans, as well as mammalian cells in culture, or small rodents. Drs...
- Deciphering the Composition of oskar mRNP via in vivo Fluorescence ImagingDIANA BRATU; Fiscal Year: 2010..is to examine the spatio-temporal requirements of trans-acting factors during mRNA transport in Drosophila melanogaster oocytes...
- Role of circadian clocks in maintaining a healthy nervous systemSVETLANA NIKOLAEVNA RADYUK; Fiscal Year: 2012..To test this hypothesis, we propose an interdisciplinary collaboration using the excellent model system Drosophila melanogaster. In aim 1, we will determine roles of circadian clocks in the regulation of glutathione biosynthesis in ..
- Genetic analysis and regulation of amyloids flux in the Drosophila heartGIRISH CHANDRA MELKANI; Fiscal Year: 2011..To test our hypothesis, we will use the genetically tractable model organism Drosophila melanogaster and employ an integrative approach in exploration and suppression of cardiac amyloidosis...
- Evolution of Drosophila melanogaster cis-regulatory modulesAaron Hardin; Fiscal Year: 2013..I use as a model early anterior-posterior patterning in Drosophila melanogaster and its relatives...
- L2DTL Function in Maintaining Genomic Stability during Drosophila DevelopmentCATHERINE SILVER KEY; Fiscal Year: 2010..Since Cdt1/Dup has been extensively studied in Drosophila in various cell and development contexts, Drosophila melanogaster serves as an excellent model for elucidating the role of the L2DTL family in regulating Cdt1/Dup levels ..
- Role of FGF Signaling in Controlling Cell Movements During Drosophila DevelopmentANGELIKE M STATHOPOULOS; Fiscal Year: 2013..We have found that only three of six possible FGF-FGFR interactions are acting in Drosophila melanogaster: Pyramus and Thisbe FGF ligands each support activation of the FGFR Heartless, while only the FGF ligand ..
- Characterization of a mutation that affects feeding and digestion in DrosophilaEDWARD BLUMENTHAL; Fiscal Year: 2009..and physiological pathways required for the regulation of digestion and feeding behavior in the fruit fly Drosophila melanogaster, an organism that has proven to be an excellent model system for studies of feeding regulation and energy ..
- Activation of Insect Immunity by Gram-negative BacteriaNeal Silverman; Fiscal Year: 2013..This proposal uses the fruit fly Drosophila melanogaster as a model for the study of innate immunity...
- CRYOPRESERVATION OF DROSOPHILA MELANOGASTER EMBRYOSPETER STEPONKUS; Fiscal Year: 1993The ultimate goal of this project is to develop a protocol for the cryopreservtion of Drosophila melanogaster embryos. We have investigated three different strategies: conventional cryopreservation, vitrification, and supercooling...
- TEST OF NEUTRAL MOLECULAR EVOLUTIONMartin Kreitman; Fiscal Year: 1999..are proposed; all are based on the collection of DNA sequence data from the closely related species Drosophila melanogaster and D. simulans, as well as the species pairs D. yakuba - D. teissieri and D. erecta - D. orena...
- EFFECTS OF AGING ON GERMLINE AND SOMATIC STEM CELLS IN DROSOPHILA MELANOGASTERMATTHEW WALLENFANG; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Mapping and rescuing EMS-induced lethal mutations on the Drosophila X ChromosomeHugo J Bellen; Fiscal Year: 2010..to map and deposit a collection of well defined chemically induced mutations in essential genes on the Drosophila melanogaster X chromosome for distribution by the Bloomington Drosophila Stock Center (BDSC) to the fly community...
- Drosophila melanogaster as a model host for Vibrio choleraePaula I Watnick; Fiscal Year: 2010..We have previously shown that ingestion of pathogenic strains of V. cholerae by Drosophila melanogaster results in lethal infection...