gene knockdown techniques

Summary

Summary: The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.

Top Publications

  1. Zhu B, Xu T, Lin Z, Wang C, Li Y, Zhao M, et al. Recombinant heat shock protein 78 enhances enterovirus 71 propagation in Vero cells and is induced in SK-N-SH cells during the infection. Arch Virol. 2017;162:1649-1660 pubmed publisher
    ..However, knockdown of the HSP78 gene in Vero cells before EV71 infection did not result in a reduced virus titer. In addition, HSP78 on the cell surface was upregulated in human neuroblastoma cells (SK-N-SH) infected with EV71. ..
  2. He M, Tan B, Vasan K, Yuan H, Cheng F, Ramos da Silva S, et al. SIRT1 and AMPK pathways are essential for the proliferation and survival of primary effusion lymphoma cells. J Pathol. 2017;242:309-321 pubmed publisher
    ..Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. ..
  3. Alghamdi T, Majumder S, Thieme K, Batchu S, White K, Liu Y, et al. Janus Kinase 2 Regulates Transcription Factor EB Expression and Autophagy Completion in Glomerular Podocytes. J Am Soc Nephrol. 2017;28:2641-2653 pubmed publisher
    ..These results also raise the possibility that therapeutically modulating TFEB activity may improve podocyte health in glomerular disease. ..
  4. Manikoth Ayyathan D, Ilic N, Gil Henn H, Blank M. Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system. Anal Biochem. 2017;531:56-59 pubmed publisher
    ..Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology. ..
  5. Wang Q, Liu H, Wang Q, Zhou F, Liu Y, Zhang Y, et al. Involvement of c-Fos in cell proliferation, migration, and invasion in osteosarcoma cells accompanied by altered expression of Wnt2 and Fzd9. PLoS ONE. 2017;12:e0180558 pubmed publisher
    ..These findings offer new clues for OS development and progression, and c-Fos may be a potential therapeutic target for OS. ..
  6. Xie H, Zhao Y, Zhou Y, Liu L, Liu Y, Wang D, et al. MiR-9 Regulates the Expression of BACE1 in Dementia Induced by Chronic Brain Hypoperfusion in Rats. Cell Physiol Biochem. 2017;42:1213-1226 pubmed publisher
    ..We conclude that miR-9 plays an important role in regulating the process of dementia induced by 2VO in rats by increasing BACE1 expression via downregulation of CREB. ..
  7. Liu P, Su J, Song X, Wang S. Activation of nuclear ?-catenin/c-Myc axis promotes oxidative stress injury in streptozotocin-induced diabetic cardiomyopathy. Biochem Biophys Res Commun. 2017;493:1573-1580 pubmed publisher
    ..The present study demonstrated that activated nuclear ?-catenin/c-Myc axis was responsible for oxidative cardiac impairment of DCM. Therefore, repressing functional nuclear ?-catenin may provide a hopeful therapeutic strategy for DCM...
  8. Kondo S, Vedanayagam J, Mohammed J, Eizadshenass S, Kan L, Pang N, et al. New genes often acquire male-specific functions but rarely become essential in Drosophila. Genes Dev. 2017;31:1841-1846 pubmed publisher
    ..Moreover, we detected widespread signatures of positive selection on young male-biased genes. Thus, young genes have a preferential impact on male reproductive system function...
  9. Wei Y, Wang Y, Zang A, Shang Y, Song Z, Wang Z, et al. Inducible T-cell co-stimulators regulate the proliferation and invasion of human hepatocellular carcinoma HepG2 cells. Biol Res. 2018;51:2 pubmed publisher
    ..01). Down-regulating ICOS expression in HepG2 cells suppressed cell proliferation and invasion. The underlying mechanism may be related to the expression of the downstream factor, PI3K/AKT. ..
  10. Li P, Liu Y, Burns N, Zhao K, Song R. SIRT1 is required for mitochondrial biogenesis reprogramming in hypoxic human pulmonary arteriolar smooth muscle cells. Int J Mol Med. 2017;39:1127-1136 pubmed publisher
    ..Polydatin improves mitochondrial biogenesis and function by enhancing SIRT1 expression in hypoxic human PASMCs. ..

Detail Information

Publications118 found, 100 shown here

  1. Zhu B, Xu T, Lin Z, Wang C, Li Y, Zhao M, et al. Recombinant heat shock protein 78 enhances enterovirus 71 propagation in Vero cells and is induced in SK-N-SH cells during the infection. Arch Virol. 2017;162:1649-1660 pubmed publisher
    ..However, knockdown of the HSP78 gene in Vero cells before EV71 infection did not result in a reduced virus titer. In addition, HSP78 on the cell surface was upregulated in human neuroblastoma cells (SK-N-SH) infected with EV71. ..
  2. He M, Tan B, Vasan K, Yuan H, Cheng F, Ramos da Silva S, et al. SIRT1 and AMPK pathways are essential for the proliferation and survival of primary effusion lymphoma cells. J Pathol. 2017;242:309-321 pubmed publisher
    ..Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. ..
  3. Alghamdi T, Majumder S, Thieme K, Batchu S, White K, Liu Y, et al. Janus Kinase 2 Regulates Transcription Factor EB Expression and Autophagy Completion in Glomerular Podocytes. J Am Soc Nephrol. 2017;28:2641-2653 pubmed publisher
    ..These results also raise the possibility that therapeutically modulating TFEB activity may improve podocyte health in glomerular disease. ..
  4. Manikoth Ayyathan D, Ilic N, Gil Henn H, Blank M. Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system. Anal Biochem. 2017;531:56-59 pubmed publisher
    ..Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology. ..
  5. Wang Q, Liu H, Wang Q, Zhou F, Liu Y, Zhang Y, et al. Involvement of c-Fos in cell proliferation, migration, and invasion in osteosarcoma cells accompanied by altered expression of Wnt2 and Fzd9. PLoS ONE. 2017;12:e0180558 pubmed publisher
    ..These findings offer new clues for OS development and progression, and c-Fos may be a potential therapeutic target for OS. ..
  6. Xie H, Zhao Y, Zhou Y, Liu L, Liu Y, Wang D, et al. MiR-9 Regulates the Expression of BACE1 in Dementia Induced by Chronic Brain Hypoperfusion in Rats. Cell Physiol Biochem. 2017;42:1213-1226 pubmed publisher
    ..We conclude that miR-9 plays an important role in regulating the process of dementia induced by 2VO in rats by increasing BACE1 expression via downregulation of CREB. ..
  7. Liu P, Su J, Song X, Wang S. Activation of nuclear ?-catenin/c-Myc axis promotes oxidative stress injury in streptozotocin-induced diabetic cardiomyopathy. Biochem Biophys Res Commun. 2017;493:1573-1580 pubmed publisher
    ..The present study demonstrated that activated nuclear ?-catenin/c-Myc axis was responsible for oxidative cardiac impairment of DCM. Therefore, repressing functional nuclear ?-catenin may provide a hopeful therapeutic strategy for DCM...
  8. Kondo S, Vedanayagam J, Mohammed J, Eizadshenass S, Kan L, Pang N, et al. New genes often acquire male-specific functions but rarely become essential in Drosophila. Genes Dev. 2017;31:1841-1846 pubmed publisher
    ..Moreover, we detected widespread signatures of positive selection on young male-biased genes. Thus, young genes have a preferential impact on male reproductive system function...
  9. Wei Y, Wang Y, Zang A, Shang Y, Song Z, Wang Z, et al. Inducible T-cell co-stimulators regulate the proliferation and invasion of human hepatocellular carcinoma HepG2 cells. Biol Res. 2018;51:2 pubmed publisher
    ..01). Down-regulating ICOS expression in HepG2 cells suppressed cell proliferation and invasion. The underlying mechanism may be related to the expression of the downstream factor, PI3K/AKT. ..
  10. Li P, Liu Y, Burns N, Zhao K, Song R. SIRT1 is required for mitochondrial biogenesis reprogramming in hypoxic human pulmonary arteriolar smooth muscle cells. Int J Mol Med. 2017;39:1127-1136 pubmed publisher
    ..Polydatin improves mitochondrial biogenesis and function by enhancing SIRT1 expression in hypoxic human PASMCs. ..
  11. Xu B, Zhou M, Qiu W, Ye J, Feng Q. CCR7 mediates human breast cancer cell invasion, migration by inducing epithelial-mesenchymal transition and suppressing apoptosis through AKT pathway. Cancer Med. 2017;6:1062-1071 pubmed publisher
    ..Our results suggested that CCR7 may regard as a therapeutic target for the breast cancer treatment. ..
  12. Howe J, Li E, Streeter S, Rahme G, Chipumuro E, Russo G, et al. MiR-338-3p regulates neuronal maturation and suppresses glioblastoma proliferation. PLoS ONE. 2017;12:e0177661 pubmed publisher
    ..Further, low miR-338-3p expression is associated with increased mortality and disease progression in patients with glioblastoma. These data identify miR-338-3p as a clinically relevant tumor suppressor in glioblastoma. ..
  13. Yi Z, Yang D, Liao X, Guo F, Wang Y, Wang X. PSME3 induces epithelial-mesenchymal transition with inducing the expression of CSC markers and immunosuppression in breast cancer. Exp Cell Res. 2017;358:87-93 pubmed publisher
    ..These findings revealed that PSME3 induces epithelial-mesenchymal transition with inducing the expression of CSC markers and influencing the tumor immune microenvironment in breast cancer. ..
  14. Ka S, Bang I, Bae E, Park B. Hepatocyte-specific sirtuin 6 deletion predisposes to nonalcoholic steatohepatitis by up-regulation of Bach1, an Nrf2 repressor. FASEB J. 2017;31:3999-4010 pubmed publisher
    ..Ka, S.-O, Bang, I. H., Bae, E. J., Park, B.-H. Hepatocyte-specific sirtuin 6 deletion predisposes to nonalcoholic steatohepatitis by up-regulation of Bach1, an Nrf2 repressor. ..
  15. Guo X, Yan F, Shan X, Li J, Yang Y, Zhang J, et al. SIRT3 inhibits Ang II-induced transdifferentiation of cardiac fibroblasts through ?-catenin/PPAR-? signaling. Life Sci. 2017;186:111-117 pubmed publisher
    ..Our findings indicate that this newly identified SIRT3/?-catenin/PPAR-? axis will provide novel insight into the understanding of the mechanism of transdifferentiation of CFs to myofibroblasts. ..
  16. Cimmino L, Dolgalev I, Wang Y, Yoshimi A, Martin G, Wang J, et al. Restoration of TET2 Function Blocks Aberrant Self-Renewal and Leukemia Progression. Cell. 2017;170:1079-1095.e20 pubmed publisher
    ..paperclip. ..
  17. Guo Y, Fu S, Li L. Autographa californica multiple nucleopolyhedrovirus ac75 is required for egress of nucleocapsids from the nucleus and formation of de novo intranuclear membrane microvesicles. PLoS ONE. 2017;12:e0185630 pubmed publisher
  18. Elamin A, Steinicke S, Oehlmann W, Braun Y, Wanas H, Shuralev E, et al. Novel drug targets in cell wall biosynthesis exploited by gene disruption in Pseudomonas aeruginosa. PLoS ONE. 2017;12:e0186801 pubmed publisher
    ..aeruginosa. In conclusion, these findings establish a link between cell wall targets and virulence of P. aeruginosa and thus may lead to development of novel drugs for the treatment of P. aeruginosa infection...
  19. Sakai T, Hatano Y, Zhang W, Fujiwara S, Nishiyori R. Knockdown of either filaggrin or loricrin increases the productions of interleukin (IL)-1?, IL-8, IL-18 and granulocyte macrophage colony-stimulating factor in stratified human keratinocytes. J Dermatol Sci. 2015;80:158-60 pubmed publisher
  20. Yamamotoya T, Nakatsu Y, Matsunaga Y, Fukushima T, Yamazaki H, Kaneko S, et al. Reduced SHARPIN and LUBAC Formation May Contribute to CCl?- or Acetaminophen-Induced Liver Cirrhosis in Mice. Int J Mol Sci. 2017;18: pubmed publisher
    ..Thus, LUBAC is essential for the survival of hepatocytes, and it is likely that reduction of LUBAC is a factor promoting hepatocyte death in addition to the direct effect of drug toxicity. ..
  21. Fischer Huchzermeyer S, Dombrowski A, Hagel C, Mautner V, Schittenhelm J, Harder A. The Cellular Retinoic Acid Binding Protein 2 Promotes Survival of Malignant Peripheral Nerve Sheath Tumor Cells. Am J Pathol. 2017;187:1623-1632 pubmed publisher
    ..These data suggest a retinoic acid-independent, non-tumor suppressor role of CRABP2 for the survival of MPNST cells in vitro. Targeting CRABP2 overexpression may represent a unique approach for the treatment of human MPNSTs. ..
  22. Nakagawa H, Hazama K, Ishida K, Komori M, Nishimura K, Matsuo S. Inhibition of PLD1 activity causes ER stress via regulation of COPII vesicle formation. Biochem Biophys Res Commun. 2017;490:895-900 pubmed publisher
    ..Taken together, these results suggest that PLD1 activity regulates COPII vesicle transport from the ER to the Golgi apparatus by regulating Sec13/31 recruitment from the cytosol to the ER membrane during COPII vesicle formation. ..
  23. Erdogan S, Doganlar Z, Doganlar O, Türkekul K, Serttas R. Inhibition of Midkine Suppresses Prostate Cancer CD133+ Stem Cell Growth and Migration. Am J Med Sci. 2017;354:299-309 pubmed publisher
    ..Therefore, suppression of endogenous expression of MDK would, in combination with traditional chemotherapy drugs, be a potential treatment for PCSCs. ..
  24. Solomon Zemler R, Sarfstein R, Werner H. Nuclear insulin-like growth factor-1 receptor (IGF1R) displays proliferative and regulatory activities in non-malignant cells. PLoS ONE. 2017;12:e0185164 pubmed publisher
    ..In conclusion, results are consistent with the concept that nuclear IGF1R fulfills important physiological and pathological roles. ..
  25. Gupta V, Rajala A, Rajala R. Non-canonical regulation of phosphatidylinositol 3-kinase gamma isoform activity in retinal rod photoreceptor cells. Cell Commun Signal. 2015;13:7 pubmed publisher
    ..Our studies suggest a functional non-canonical PI3Kγ activation in retinal rod photoreceptor cells. ..
  26. Tu M, Cai L, Zheng W, Su Z, Chen Y, Qi S. CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma. Mol Med Rep. 2017;15:1713-1721 pubmed publisher
  27. Jang J, Park K, Lee J, Ko K, Kang S, Kim I. Selenoprotein S is required for clearance of C99 through endoplasmic reticulum-associated degradation. Biochem Biophys Res Commun. 2017;486:444-450 pubmed publisher
    ..These results suggest that SelS is required for C99 degradation through ERAD, resulting in inhibition of A? production. ..
  28. Jiang G, Wang P, Wang W, Li W, Dai L, Chen K. Annexin A13 promotes tumor cell invasion in vitro and is associated with metastasis in human colorectal cancer. Oncotarget. 2017;8:21663-21673 pubmed publisher
    ..ANXA13 is associated with CRC cell invasion in vitro, and with lymph node metastasis and poor survival in CRC patients. Our results indicate that ANXA13 can be exploited as a biomarker for its diagnostic and prognostic values. ..
  29. An Y, Wang G, Diao Y, Long Y, Fu X, Weng M, et al. A Molecular Switch Regulating Cell Fate Choice between Muscle Progenitor Cells and Brown Adipocytes. Dev Cell. 2017;41:382-391.e5 pubmed publisher
    ..Thus, the MyoD/Myf5-E2F4/p107/p130 axis functions in both the Pax7+/Myf5+ embryonic progenitor cells and postnatal myoblasts to repress the alternative BA fate. ..
  30. Oike A, Kodama M, Yasumasu S, Yamamoto T, Nakamura Y, Ito E, et al. Participation of androgen and its receptor in sex determination of an amphibian species. PLoS ONE. 2017;12:e0178067 pubmed publisher
    ..These results suggest that AR with its androgen ligand functions as a male sex-determinant in the ZW type R. rugosa frogs. ..
  31. Ha Y, Tak K, Kim C, Roh S, Choi E, Cho D, et al. PSMB8 as a Candidate Marker of Responsiveness to Preoperative Radiation Therapy in Rectal Cancer Patients. Int J Radiat Oncol Biol Phys. 2017;98:1164-1173 pubmed publisher
    ..These results suggest that PSMB8 is a predictive marker of preoperative radiosensitivity in LARC patients. Clinical validation in a larger cohort is now required. ..
  32. Sobkowicz A, Sanders A, Mason M, Jiang W. Potential Implication of Paxillin in Cancer Establishment Within the Bone Environment. Anticancer Res. 2017;37:4255-4268 pubmed
    ..The responsiveness of cells to environmental factors such as HGF or BME may be influenced by paxillin status, although this seems to be dependent on cell type. ..
  33. Huang T, Lee P, Wu M, Huang C, Ko C, Lee Y, et al. Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells. PLoS ONE. 2017;12:e0181034 pubmed publisher
    ..Altogether, our study showed that, for the first time, different Wnt5a mRNA isoforms play distinct roles in CRC and can be used as novel prognostic markers for CRC in the future. ..
  34. Tuesta L, Chen Z, Duncan A, Fowler C, Ishikawa M, Lee B, et al. GLP-1 acts on habenular avoidance circuits to control nicotine intake. Nat Neurosci. 2017;20:708-716 pubmed publisher
    ..GLP-1 neurons may therefore serve as 'satiety sensors' for nicotine that stimulate habenular systems to promote nicotine avoidance before its aversive effects are encountered. ..
  35. He Q, Sun C, Lei W, Ma J. SOCS1 Regulates Apoptosis and Inflammation by Inhibiting IL-4 Signaling in IL-1?-Stimulated Human Osteoarthritic Chondrocytes. Biomed Res Int. 2017;2017:4601959 pubmed publisher
    ..These findings provide a potential therapeutic target for the clinical treatment of OA. ..
  36. Jimenez L, Lim J, Burd B, Harris T, Ow T, Kawachi N, et al. miR-375 Regulates Invasion-Related Proteins Vimentin and L-Plastin. Am J Pathol. 2017;187:1523-1536 pubmed publisher
    ..Furthermore, knockdown of RUNX1, L-plastin, and vimentin resulted in significant reductions in cell invasion in vitro, indicating the functional significance of miR-375 regulation of specific proteins involved in HNSCC invasion. ..
  37. Gao H, Li D, Yang P, Zhao L, Wei L, Chen Y, et al. Suppression of CD36 attenuates adipogenesis with a reduction of P2X7 expression in 3T3-L1 cells. Biochem Biophys Res Commun. 2017;491:204-208 pubmed publisher
    ..In addition, purinergic receptor P2X, ligand-gated ion channel 7 (P2X7) was downregulated in CD36-knockdown 3T3-L1 cells, suggesting that the suppression of CD36 attenuates adipogenesis via the P2X7 pathway in 3T3-L1 cells. ..
  38. Jin J, Chu Z, Ma P, Meng Y, Yang Y. Long non-coding RNA SPRY4-IT1 promotes proliferation and invasion by acting as a ceRNA of miR-101-3p in colorectal cancer cells. Tumour Biol. 2017;39:1010428317716250 pubmed publisher
    ..The bioinformatics prediction revealed putative miR-101-3p binding sites within SPRY4-IT1 transcripts. Above all, knockdown of SPRY4-IT1 could represent a rational therapeutic strategy for colorectal carcinoma. ..
  39. Mascarenhas D, Cerqueira D, Pereira M, Castanheira F, Fernandes T, Manin G, et al. Inhibition of caspase-1 or gasdermin-D enable caspase-8 activation in the Naip5/NLRC4/ASC inflammasome. PLoS Pathog. 2017;13:e1006502 pubmed publisher
    ..Our data suggest that caspase-8 activation in the Naip5/NLRC4/ASC inflammasome enable induction of cell death when caspase-1 or gasdermin-D is suppressed. ..
  40. Britton E, Rogerson C, Mehta S, Li Y, Li X, Fitzgerald R, et al. Open chromatin profiling identifies AP1 as a transcriptional regulator in oesophageal adenocarcinoma. PLoS Genet. 2017;13:e1006879 pubmed publisher
  41. Serrano Flores B, Garay E, Vázquez Cuevas F, Arellano R. Differential role of STIM1 and STIM2 during transient inward (T in) current generation and the maturation process in the Xenopus oocyte. BMC Physiol. 2014;14:9 pubmed publisher
    ..Thus, the endogenous SOCE response in Xenopus oocytes depended mainly on STIM2, and its expression was necessary for entry into meiosis induced by progesterone. ..
  42. Xiao L, Kan W. Poly ADP-ribose polymerase inhibition suppresses cisplatin toxicity in chronic myeloid leukemia cells. Anticancer Drugs. 2017;28:316-321 pubmed publisher
    ..These results indicated that the toxicity of cisplatin in CML cells requires PARP activity. Therefore, PARP inhibitors may not be useful with DNA-damaging agents such as cisplatin in CML treatment. ..
  43. Fujino T, Sakamaki R, Ito H, Furusato Y, Sakamoto N, Oshima T, et al. Farnesoid X receptor regulates the growth of renal adenocarcinoma cells without affecting that of a normal renal cell-derived cell line. J Toxicol Sci. 2017;42:259-265 pubmed publisher
    ..Our study showed that FXR stimulates proliferation of renal adenocarcinoma cells and that FXR knockdown is useful for growth suppression of renal adenocarcinoma without cytotoxicity to normal renal cells. ..
  44. Zhang X, Lin D, Lin Y, Chen H, Zou M, Zhong S, et al. Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b. Tumour Biol. 2017;39:1010428317705767 pubmed publisher
    ..Our findings, for the first time, indicated that the miR-148b/proteasome beta-4 subunit axis contributed to the development of melanoma, revealing novel therapeutic targets for the treatment of melanoma. ..
  45. Zhang Y, Kurupati R, Liu L, Zhou X, Zhang G, Hudaihed A, et al. Enhancing CD8+ T Cell Fatty Acid Catabolism within a Metabolically Challenging Tumor Microenvironment Increases the Efficacy of Melanoma Immunotherapy. Cancer Cell. 2017;32:377-391.e9 pubmed publisher
    ..PD-1 blockade delays tumor growth without changing TIL metabolism or functions. It synergizes with metabolic reprogramming of T cells to achieve superior antitumor efficacy and even complete cures. ..
  46. Chao C, Chang C, Wu M, Ko H, Wang D, Hung M, et al. MicroRNA-205 signaling regulates mammary stem cell fate and tumorigenesis. J Clin Invest. 2014;124:3093-106 pubmed publisher
    ..This study elucidates an important role for miR-205 in the regulation of mammary stem cell fate, suggesting a potential therapeutic target for limiting breast cancer genesis. ..
  47. Zhu S, Huang J, Peng W, Wu D, Luo M, Li Q, et al. Inhibition of smoothened decreases proliferation of synoviocytes in rheumatoid arthritis. Cell Mol Immunol. 2017;14:214-222 pubmed publisher
    ..05). The results suggest that Shh signaling plays an important role in RA-FLSs proliferation in a Smo-dependent manner and may contribute to synovial hyperplasia. Targeting Shh signaling may help control joint damage in patients with RA. ..
  48. Qian Y, Wong C, Xu J, Chen H, Zhang Y, Kang W, et al. Sodium Channel Subunit SCNN1B Suppresses Gastric Cancer Growth and Metastasis via GRP78 Degradation. Cancer Res. 2017;77:1968-1982 pubmed publisher
    ..i>Cancer Res; 77(8); 1968-82. ©2017 AACR. ..
  49. Yokoyama Y, Watanabe T, Tamura Y, Hashizume Y, Miyazono K, Ehata S. Autocrine BMP-4 Signaling Is a Therapeutic Target in Colorectal Cancer. Cancer Res. 2017;77:4026-4038 pubmed publisher
    ..Our findings suggest inhibition of autocrine BMP-4 as a candidate treatment strategy for colorectal cancer. Cancer Res; 77(15); 4026-38. ©2017 AACR. ..
  50. Satkunanathan S, Thorpe R, Zhao Y. The function of DNA binding protein nucleophosmin in AAV replication. Virology. 2017;510:46-54 pubmed publisher
    ..Understanding the role of cellular proteins in the AAV life cycle will greatly facilitate high titre production of AAV vectors for clinical use. ..
  51. Bansod S, Kageyama R, Ohtsuka T. Hes5 regulates the transition timing of neurogenesis and gliogenesis in mammalian neocortical development. Development. 2017;144:3156-3167 pubmed publisher
  52. Chen L, Yang W, Guo Y, Chen W, Zheng P, Zeng J, et al. Exosomal lncRNA GAS5 regulates the apoptosis of macrophages and vascular endothelial cells in atherosclerosis. PLoS ONE. 2017;12:e0185406 pubmed publisher
  53. Cheng J, Chang H, Leung P. TGF-?1 Inhibits Human Trophoblast Cell Invasion by Upregulating Connective Tissue Growth Factor Expression. Endocrinology. 2017;158:3620-3628 pubmed publisher
    ..Furthermore, cell invasiveness is decreased by treatment with recombinant CTGF. These results provide evidence that CTGF mediates TGF-?1-inhibited human trophoblast cell invasion. ..
  54. Oguchi M, Noguchi K, Fukuda M. TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS ONE. 2017;12:e0174883 pubmed publisher
    ..These findings indicated that TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. ..
  55. Xie X, Zhan X, Hu Z. [Zinc finger protein 521 suppresses osteogenic differentiation of rat mesenchymal stem cells by inhibiting the Wnt/beta-catenin signaling pathway]. Mol Biol (Mosk). 2017;51:464-472 pubmed publisher
    ..Therefore, we conclude that Zfp521 regulates osteogenic differentiation of rat MSCs through the suppression of the Wnt/beta-catenin signaling pathway. ..
  56. Moore K, Thomas G, Duffy S, Warwick J, Gabe R, Chou P, et al. Therapeutic targeting of integrin ?v?6 in breast cancer. J Natl Cancer Inst. 2014;106: pubmed publisher
    ..Targeting ?v?6 with 264RAD alone or in combination with trastuzumab may provide a novel therapy for treating high-risk and trastuzumab-resistant breast cancer patients. ..
  57. Posey K, Coustry F, Veerisetty A, Hossain M, Gattis D, Booten S, et al. Antisense Reduction of Mutant COMP Reduces Growth Plate Chondrocyte Pathology. Mol Ther. 2017;25:705-714 pubmed publisher
    ..This novel and exciting work demonstrates that antisense-based therapy is a viable approach for treating pseudoachondroplasia and other human cartilage disorders. ..
  58. Basu A, Sridharan S. Regulation of anti-apoptotic Bcl-2 family protein Mcl-1 by S6 kinase 2. PLoS ONE. 2017;12:e0173854 pubmed publisher
    ..JNK1 knockdown also had a modest effect in attenuating the increase in doxorubicin-induced apoptosis caused by S6K2 deficiency. These results suggest that S6K2 regulates apoptosis via multiple mechanisms, and involves both Akt and JNK. ..
  59. Yang Z, Lv Y, Wang L, Chen Y, Han J, Zhao S, et al. Inhibition of hedgehog pathway reveals the regulatory role of SMO in gastric cancer cells. Tumour Biol. 2017;39:1010428317715546 pubmed publisher
    ..Our studies demonstrate an important role for smoothened and glioma-associated oncogene homology-1 in gastric cancer and suggest inhibition of hedgehog pathway as a novel and potent strategy to treat gastric cancer patients. ..
  60. Grimaldo L, Sandoval A, Garza López E, Felix R. Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels. PLoS ONE. 2017;12:e0185289 pubmed publisher
    ..Together these results unveil a novel functional coupling between Parkin and the CaV2.2 channels and provide a novel insight into the basic mechanisms of CaV channels protein quality control and functional expression. ..
  61. Lombardi M, Gillieron C, Berkelaar M, Gabay C. Salt-inducible kinases (SIK) inhibition reduces RANKL-induced osteoclastogenesis. PLoS ONE. 2017;12:e0185426 pubmed publisher
    ..SIK kinase inhibitors could thus represent a potential novel therapy to prevent bone erosions...
  62. Lohr J, Meinzer F, Dalla S, Romey Glüsing R, Dobler S. The function and evolutionary significance of a triplicated Na,K-ATPase gene in a toxin-specialized insect. BMC Evol Biol. 2017;17:256 pubmed publisher
    ..Gene duplications of the Na,K-ATPase thus represent an excellent example of subfunctionalization in response to a new environmental challenge. ..
  63. Isaji T, Im S, Gu W, Wang Y, Hang Q, Lu J, et al. An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J Biol Chem. 2014;289:20694-705 pubmed
    ..These results are the first direct demonstration of the role of GOLPH3 in N-glycosylation to regulate cell biological functions. ..
  64. Putiri E, Tiedemann R, Thompson J, Liu C, Ho T, Choi J, et al. Distinct and overlapping control of 5-methylcytosine and 5-hydroxymethylcytosine by the TET proteins in human cancer cells. Genome Biol. 2014;15:R81 pubmed publisher
    ..These results provide novel insight into the division of labor among TET proteins and reveal important connections between TET activity, the chromatin landscape, and gene expression. ..
  65. Ge C, Ye J, Zhang H, Zhang Y, Sun W, Sang Y, et al. Dmrt1 induces the male pathway in a turtle species with temperature-dependent sex determination. Development. 2017;144:2222-2233 pubmed publisher
  66. Tan M, Zhang D, Zhang E, Xu D, Liu Z, Qiu J, et al. SENP2 suppresses epithelial-mesenchymal transition of bladder cancer cells through deSUMOylation of TGF-?RI. Mol Carcinog. 2017;56:2332-2341 pubmed publisher
    ..Our results establish a function of SENP2 in metastatic progression and suggest its candidacy as a new prognostic biomarker and target for clinical management of bladder cancer. ..
  67. Wang L, Qi Y, Xiong Y, Peng Z, Ma Q, Zhang Y, et al. Ezrin-Radixin-Moesin Binding Phosphoprotein 50 (EBP50) Suppresses the Metastasis of Breast Cancer and HeLa Cells by Inhibiting Matrix Metalloproteinase-2 Activity. Anticancer Res. 2017;37:4353-4360 pubmed
    ..Our work reveals the anti-metastatic effect and a new mechanism of EBP50 action in breast and cervical cancer cells. ..
  68. Liu Y, Zong Z, Guan X, Wang L, Zhao Y. The role of long non-coding RNA PCA3 in epithelial ovarian carcinoma tumorigenesis and progression. Gene. 2017;633:42-47 pubmed publisher
    ..PCA3 may be a novel and important diagnostic biomarker and a valuable marker for prediction in the clinical care of epithelial ovarian carcinoma. ..
  69. Chu H, McIver E, Kovaleski R, Atherton J, Bevan M. Loss of Hyperdirect Pathway Cortico-Subthalamic Inputs Following Degeneration of Midbrain Dopamine Neurons. Neuron. 2017;95:1306-1318.e5 pubmed publisher
    ..Together, the data suggest that loss of dopamine triggers a maladaptive shift in the balance of synaptic excitation and inhibition in the STN, which contributes to parkinsonian activity and motor dysfunction. ..
  70. Cherry J, DiDonato C, Androphy E, Calo A, Potter K, Custer S, et al. In vitro and in vivo effects of 2,4 diaminoquinazoline inhibitors of the decapping scavenger enzyme DcpS: Context-specific modulation of SMN transcript levels. PLoS ONE. 2017;12:e0185079 pubmed publisher
    ..We conclude that DAQ-DcpSi have reproducible benefit in SMA mice and a broad spectrum of biological effects in vitro and in vivo, but these are complex, context specific, and not the result of simple SMN2 transcriptional activation. ..
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    ..Collectively, our data reveal the roles and functional mechanisms of HOTAIR in human chondrosarcoma and suggest that HOTAIR may act as a prognostic biomarker and potential therapeutic target for chondrosarcoma. ..
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    ..Targeting of single nucleotide changes in other genes yielded analogous results. These results support our enrichment method as an efficient tool in genome editing. ..
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    ..We propose that genome-wide sgRNA screens based on plasmids coupled with WGS are powerful tools for forward genetics studies and drug target discovery. ..
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    ..The activation of ROCK1 was repressed by MYBPH. Luminal delivery of MYBPH adenovirus to carotid arteries decreased neointimal hyperplasia in vivo. MYBPH may, therefore, serve as a novel therapeutic target for postangioplasty restenosis. ..
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    ..SIRT1 has been shown to have a neuroprotective effect; however, its working mechanisms are not well understood. This study aimed to address this issue...
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    ..Collectively, our findings reveal that p53-induced lncRNA TRINGS controls the necrotic pathway and contributes to the survival of cancer cells harboring wild-type p53 under glucose stress. ..
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    ..These results shed new light on the role of Spp1 in nerve degeneration and regeneration during WD. ..
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    ..These findings underscore an important role for HuR in sculpting the molecular signature and phenotype of activated microglia, and as a possible therapeutic target in ALS and other neurodegenerative diseases. ..
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    ..Finally, we show that NMS-873, a small molecule inhibitor of VCP, is a potent HCMV antiviral with potential as a novel host targeting therapeutic for HCMV infection. ..
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    ..If additional studies in mice and in pilot human trials confirm our results, inhibition of P2Y12 might be a new therapeutic option that can be used in adjuvant to the traditional surgery and chemotherapy in patients with ovarian cancer. ..
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    ..Taken together, this study reveals a crucial role of CRNDE in the pathology of hepatoblastoma. CRNDE may serve as a promising diagnostic marker and therapeutic target for hepatoblastoma. ..
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    ..The anti-inflammation and growth inhibition effects exhibited by enhanced SOCS3 in OA appear to be related to its capacity to down-regulate expression levels of NF-?B and COX2. ..
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    ..Overexpression of miR-199a/b-5p decreases GCNT2 expression and suppresses I antigen production. Based on these findings, we propose that miR-199a/b-5p regulates GCNT2 and I antigen expression in colon cancer cells undergoing EMT. ..
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    ..Collectively, these results indicate that Smac plays an important role in ERS-induced apoptosis in HLECs, suggesting its close association with cataract development. ..
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    ..Thus, our results help to delineate new strategies against P. aeruginosa infections, simultaneously targeting ?-lactam resistance, cell-wall metabolism and virulence, ultimately enhancing the activity of our innate immune weapons. ..
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    ..Thus, the polarized secretion of Wnt1 is regulated by different mechanisms than other Wnts...