hemolysis

Summary

Summary: The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.

Top Publications

  1. ncbi In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis
    Dagmar Fischer
    Department of Pharmaceutics and Biopharmacy, University of Marburg, Ketzerbach 63, 35032, Marburg, Germany
    Biomaterials 24:1121-31. 2003
  2. pmc Lactate dehydrogenase as a biomarker of hemolysis-associated nitric oxide resistance, priapism, leg ulceration, pulmonary hypertension, and death in patients with sickle cell disease
    Gregory J Kato
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Dr, MSC 1476, Bldg 10CRC, Rm 5 5140, Bethesda, MD 20892, USA
    Blood 107:2279-85. 2006
  3. ncbi Understanding the mechanisms for metabolism-linked hemolytic toxicity of primaquine against glucose 6-phosphate dehydrogenase deficient human erythrocytes: evaluation of eryptotic pathway
    Shobana Ganesan
    National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, United States
    Toxicology 294:54-60. 2012
  4. ncbi Sunitinib-sensitive suicidal erythrocyte death
    Nazneen Shaik
    Department of Physiology, University of Tuebingen, Tuebingen, Germany
    Cell Physiol Biochem 30:512-22. 2012
  5. ncbi Tanshinone IIA stimulates erythrocyte phosphatidylserine exposure
    Christine Zelenak
    Department of Physiology, University of Tuebingen, Tuebingen, Germany
    Cell Physiol Biochem 30:282-94. 2012
  6. ncbi Enhanced erythrocyte membrane exposure of phosphatidylserine following sorafenib treatment: an in vivo and in vitro study
    Adrian Lupescu
    Department of Physiology, Tuebingen, Tubingen, Germany
    Cell Physiol Biochem 30:876-88. 2012
  7. pmc Hemolysis and free hemoglobin revisited: exploring hemoglobin and hemin scavengers as a novel class of therapeutic proteins
    Dominik J Schaer
    Division of Internal Medicine, University Hospital, Zurich, Switzerland
    Blood 121:1276-84. 2013
  8. ncbi Carbon monoxide-sensitive apoptotic death of erythrocytes
    Elisabeth Lang
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Basic Clin Pharmacol Toxicol 111:348-55. 2012
  9. ncbi In vitro effect of CTAB- and PEG-coated gold nanorods on the induction of eryptosis/erythroptosis in human erythrocytes
    Irene P Lau
    Programme of Biochemistry, School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong
    Nanotoxicology 6:847-56. 2012
  10. ncbi Stimulation of suicidal erythrocyte death by benzethonium
    Elisabeth Lang
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 28:347-54. 2011

Detail Information

Publications342 found, 100 shown here

  1. ncbi In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis
    Dagmar Fischer
    Department of Pharmaceutics and Biopharmacy, University of Marburg, Ketzerbach 63, 35032, Marburg, Germany
    Biomaterials 24:1121-31. 2003
    ..Microscopic observations were carried out as indicators for cell viability. Furthermore, hemolysis of erythrocytes was quantified spectrophotometrically...
  2. pmc Lactate dehydrogenase as a biomarker of hemolysis-associated nitric oxide resistance, priapism, leg ulceration, pulmonary hypertension, and death in patients with sickle cell disease
    Gregory J Kato
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Dr, MSC 1476, Bldg 10CRC, Rm 5 5140, Bethesda, MD 20892, USA
    Blood 107:2279-85. 2006
    ..in adult patients with sickle cell disease and is strongly associated with early mortality and markers of hemolysis, in particular, serum lactate dehydrogenase (LDH)...
  3. ncbi Understanding the mechanisms for metabolism-linked hemolytic toxicity of primaquine against glucose 6-phosphate dehydrogenase deficient human erythrocytes: evaluation of eryptotic pathway
    Shobana Ganesan
    National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, United States
    Toxicology 294:54-60. 2012
    ..The studies suggest that eryptosis mechanisms are not involved in accelerated removal of erythrocytes due to hemolytic toxicity of primaquine...
  4. ncbi Sunitinib-sensitive suicidal erythrocyte death
    Nazneen Shaik
    Department of Physiology, University of Tuebingen, Tuebingen, Germany
    Cell Physiol Biochem 30:512-22. 2012
    ..from Fluo-3-fluorescence, cell volume from forward scatter, phosphatidylserine-exposure from annexin-V-binding, hemolysis from hemoglobin release, ceramide abundance from anti-ceramide antibody binding, and cytosolic ATP from ..
  5. ncbi Tanshinone IIA stimulates erythrocyte phosphatidylserine exposure
    Christine Zelenak
    Department of Physiology, University of Tuebingen, Tuebingen, Germany
    Cell Physiol Biochem 30:282-94. 2012
    ..cell volume from forward scatter, phosphatidylserine exposure from binding of fluorescent annexin V, hemolysis from hemoglobin concentration in the supernatant, ATP concentration utilizing luciferin-luciferase and ceramide ..
  6. ncbi Enhanced erythrocyte membrane exposure of phosphatidylserine following sorafenib treatment: an in vivo and in vitro study
    Adrian Lupescu
    Department of Physiology, Tuebingen, Tubingen, Germany
    Cell Physiol Biochem 30:876-88. 2012
    ..Signaling leading to eryptosis include increase in cytosolic Ca(2+)activity ([Ca(2+)](i)), formation of ceramide, ATP-depletion and oxidative stress. The present study explored, whether sorafenib triggers eryptosis in vitro and in vivo...
  7. pmc Hemolysis and free hemoglobin revisited: exploring hemoglobin and hemin scavengers as a novel class of therapeutic proteins
    Dominik J Schaer
    Division of Internal Medicine, University Hospital, Zurich, Switzerland
    Blood 121:1276-84. 2013
    b>Hemolysis occurs in many hematologic and nonhematologic diseases...
  8. ncbi Carbon monoxide-sensitive apoptotic death of erythrocytes
    Elisabeth Lang
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Basic Clin Pharmacol Toxicol 111:348-55. 2012
    ..In conclusion, CORM-2 stimulates cell membrane scrambling of erythrocytes, an effect largely due to Ca²⁺ entry and partially reversed by O₂...
  9. ncbi In vitro effect of CTAB- and PEG-coated gold nanorods on the induction of eryptosis/erythroptosis in human erythrocytes
    Irene P Lau
    Programme of Biochemistry, School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong
    Nanotoxicology 6:847-56. 2012
    ..Mechanistically, cytosolic Ca(2+) ions seem to be the key mediator in the eryptosis/erythroptosis mediated by the CTAB or CTAB-Au-NRs while caspase-3 and reactive oxygen species did not contribute much to the process...
  10. ncbi Stimulation of suicidal erythrocyte death by benzethonium
    Elisabeth Lang
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 28:347-54. 2011
    ..cell volume from forward scatter in FACS analysis, cytosolic Ca(2+)-concentration from Fluo3-fluorescence, hemolysis from hemoglobin release, lactate formation by colorimetry and ceramide utilizing fluorescent antibodies...
  11. ncbi Induction of apoptotic erythrocyte death by rotenone
    Adrian Lupescu
    Department of Physiology, University of Tuebingen, Gmelinstrasse 5, 72076 Tuebingen, Germany
    Toxicology 300:132-7. 2012
    ..scatter, phosphatidylserine-exposure from annexin-V-binding, ceramide utilizing fluorescence antibodies and hemolysis from hemoglobin release...
  12. ncbi Stimulation of suicidal death of erythrocytes by rifampicin
    Majed Abed
    Department of Physiology, University of Tuebingen, Tuebingen, Germany
    Toxicology 302:123-8. 2012
    ..flow cytometry, PS exposure from annexin binding, ceramide formation from binding of fluorescent antibodies and hemolysis from hemoglobin release...
  13. ncbi Withaferin A-stimulated Ca2+ entry, ceramide formation and suicidal death of erythrocytes
    Kashif Jilani
    Department of Physiology, University of Tuebingen, Gmelinstraße 5, 72076 Tuebingen, Germany
    Toxicol In Vitro 27:52-8. 2013
    ..from Fluo3-fluorescence, cell volume from forward scatter, phosphatidylserine exposure from annexin-V-binding, hemolysis from hemoglobin release, oxidative stress from DCFDA-fluorescence and ceramide abundance utilizing antibodies...
  14. pmc Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction
    Aubrey J Cunnington
    Department of Immunology and Infection, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    J Immunol 189:5336-46. 2012
    ..bacterial infections such as nontyphoid Salmonella during and after a malaria infection, but in mice, malarial hemolysis impairs resistance to nontyphoid Salmonella by impairing the neutrophil oxidative burst...
  15. ncbi Hemolyzed specimens: a major challenge for emergency departments and clinical laboratories
    Giuseppe Lippi
    U O Diagnostica Ematochimica, Azienda Ospedaliero Universitaria di Parma, Parma, Italy
    Crit Rev Clin Lab Sci 48:143-53. 2011
    The term hemolysis designates the pathological process of breakdown of red blood cells in blood, which is typically accompanied by varying degrees of red tinge in serum or plasma once the whole blood specimen has been centrifuged...
  16. pmc Hemoglobin-driven pathophysiology is an in vivo consequence of the red blood cell storage lesion that can be attenuated in guinea pigs by haptoglobin therapy
    Jin Hyen Baek
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, FDA, Bethesda, Maryland 20892, USA
    J Clin Invest 122:1444-58. 2012
    ..Transfusion with old but not new blood led to intravascular hemolysis, acute hypertension, vascular injury, and kidney dysfunction associated with pathophysiology driven by ..
  17. pmc The relationship between the severity of hemolysis, clinical manifestations and risk of death in 415 patients with sickle cell anemia in the US and Europe
    Mehdi Nouraie
    Howard University, Washington, USA
    Haematologica 98:464-72. 2013
    ..A composite variable derived from several individual markers of hemolysis could facilitate studies of the underlying mechanisms of hemolysis...
  18. ncbi Glucose-6-phosphate dehydrogenase deficiency and antimalarial drug development
    Ernest Beutler
    The Scripps Research Institute, La Jolla, California 92037, USA
    Am J Trop Med Hyg 77:779-89. 2007
    ..This deficiency appears to provide some protection from this infection, but it can also cause hemolysis after administration of some antimalarial drugs, especially primaquine...
  19. pmc Deconstructing sickle cell disease: reappraisal of the role of hemolysis in the development of clinical subphenotypes
    Gregory J Kato
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, Critical Care Medicine Department, Clinical Center, National Institutes of Health, 10 Center Drive, Building 10CRC 5 5140, Bethesda, MD 20892 1476, USA
    Blood Rev 21:37-47. 2007
    b>Hemolysis, long discounted as a critical measure of sickle cell disease severity when compared with sickle vaso-occlusion, may be the proximate cause of some disease complications...
  20. ncbi Multicenter evaluation of the hemolysis index in automated clinical chemistry systems
    Giuseppe Lippi
    Clinical Chemistry Laboratory, University of Verona, Verona, Italy
    Clin Chem Lab Med 47:934-9. 2009
    In vitro hemolysis, the prevailing cause of preanalytical error in routine laboratory diagnostics, might influence the reliability of several tests, affect the quality of the total testing process and jeopardize patient safety...
  21. ncbi The clinical sequelae of intravascular hemolysis and extracellular plasma hemoglobin: a novel mechanism of human disease
    Russell P Rother
    Alexion Pharmaceuticals, Cheshire, Conn 06410, USA
    JAMA 293:1653-62. 2005
    ..A growing list of clinical manifestations attributed to hemoglobin release in a variety of acquired and iatrogenic hemolytic disorders suggests that hemolysis and hemoglobinemia should be considered as a novel mechanism of human disease.
  22. ncbi Tumor necrosis factor-alpha mediates hemolysis-induced vasoconstriction and the cerebral vasospasm evoked by subarachnoid hemorrhage
    Carmine Vecchione
    Department of AngioCardioNeurology, Neuromed Institute IRCCS, Pozzilli, Italy
    Hypertension 54:150-6. 2009
    ..that the proinflammatory cytokine tumor necrosis factor-alpha contributes to vascular oxidative stress evoked by hemolysis. We observed that hemolysis induces a significant increase in tumor necrosis factor-alpha both in blood and in ..
  23. pmc Impact of intravascular hemolysis in malaria on liver dysfunction: involvement of hepatic free heme overload, NF-κB activation, and neutrophil infiltration
    Sumanta Dey
    Division of Infectious Diseases and Immunology, Council of Scientific and Industrial Research Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Kolkata 700032, West Bengal, India
    J Biol Chem 287:26630-46. 2012
    We have investigated the impact of persistent intravascular hemolysis on liver dysfunction using the mouse malaria model...
  24. ncbi Key residues for membrane binding, oligomerization, and pore forming activity of staphylococcal alpha-hemolysin identified by cysteine scanning mutagenesis and targeted chemical modification
    B Walker
    Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545, USA
    J Biol Chem 270:23065-71. 1995
    ....
  25. ncbi Cytotoxicity, hemolysis, and acute in vivo toxicity of dendrimers based on melamine, candidate vehicles for drug delivery
    Hui Ting Chen
    Department of Chemistry, Texas A and M University, College Station, Texas 77843 3255, USA
    J Am Chem Soc 126:10044-8. 2004
    ..No toxicity--neither mortality nor abnormal blood chemistry based on blood urea nitrogen levels or alanine transaminase activity--was observed in doses up to 2.56 g/kg i.p. and 1.28 g/kg i.v...
  26. ncbi Amphipathic alpha helical antimicrobial peptides
    A Giangaspero
    Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Italy
    Eur J Biochem 268:5589-600. 2001
    ..The simple guidelines obtained in this study allowed the design of highly active shortened AMPs and may be generally useful in the development of this type of peptides as anti-infective agents...
  27. ncbi Cumulative effects of consecutive running sessions on hemolysis, inflammation and hepcidin activity
    Peter Peeling
    School of Sport Science, Exercise and Health, The University of Western Australia, Crawley, WA, Australia
    Eur J Appl Physiol 106:51-9. 2009
    The effect of two running sessions completed within a 12-h period on hemolysis, inflammation, and hepcidin activity in endurance athletes was investigated...
  28. ncbi Erythrocyte density in sickle cell syndromes is associated with specific clinical manifestations and hemolysis
    Pablo Bartolucci
    Service de Medecine Interne, Hopital Henri Mondor, Assistance Publique Hopitaux de Paris AP HP, Universite Paris Est, Creteil, France
    Blood 120:3136-41. 2012
    ..Thus, DRBCs are associated with specific clinical manifestations and biologic markers and may be a useful addition to the biologic and clinical evaluation of patients with SCD, because they can easily be measured in a hematocrit tube...
  29. pmc The tripartite type III secreton of Shigella flexneri inserts IpaB and IpaC into host membranes
    A Blocker
    Unité de Pathogénie Microbienne Moléculaire INSERM U389, Institut Pasteur, 75724 Paris Cedex 15, France
    J Cell Biol 147:683-93. 1999
    ..We used the contact hemolytic activity of Shigella flexneri to investigate its putative translocator. Hemolysis was caused by formation of a 25-A pore within the red blood cell (RBC) membrane...
  30. pmc In Tanzania, hemolysis after a single dose of primaquine coadministered with an artemisinin is not restricted to glucose-6-phosphate dehydrogenase-deficient (G6PD A-) individuals
    Seif A Shekalaghe
    Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands
    Antimicrob Agents Chemother 54:1762-8. 2010
    ..Despite concerns about PQ-induced hemolysis in glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals, a single dose of PQ may be safe and ..
  31. ncbi A novel protein toxin from the deadly box jellyfish (Sea Wasp, Habu-kurage) Chiropsalmus quadrigatus
    Hiroshi Nagai
    Suntory Institute for Bioorganic Research, Mishima, Osaka, Japan
    Biosci Biotechnol Biochem 66:97-102. 2002
    ..The deduced amino acid sequence of CqTX-A (462 amino acids) showed 25.2% and 21.6% sequence similarity to Carybdea rastoni toxins (CrTXs) and Carybdea alata toxin-A (CrTX-A), respectively, which are Cubozoan jellyfish toxins...
  32. ncbi Isolation and characterization of a novel protein toxin from the Hawaiian box jellyfish (sea wasp) Carybdea alata
    H Nagai
    Suntory Institute for Bioorganic Research, 1 1 1, Wakayamadai, Shimamoto, Mishima, Osaka, 618 8503, Japan
    Biochem Biophys Res Commun 275:589-94. 2000
    ..Secondary structure analysis of CaTX-A and CrTXs suggested the presence of amphiphilic alpha-helices, which are also seen in several known hemolytic or cytolytic protein toxins, including peptide toxins...
  33. ncbi Haemolysis: an overview of the leading cause of unsuitable specimens in clinical laboratories
    Giuseppe Lippi
    Clinical Chemistry Laboratory, University of Verona, Verona, Italy
    Clin Chem Lab Med 46:764-72. 2008
    ....
  34. pmc Effects of net charge and the number of positively charged residues on the biological activity of amphipathic alpha-helical cationic antimicrobial peptides
    Ziqing Jiang
    Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA
    Biopolymers 90:369-83. 2008
    ..and gram-positive bacteria were determined along with the maximal peptide concentration that produces no hemolysis of human red blood cells (MHC)...
  35. ncbi Cerebral malaria and the hemolysis/methemoglobin/heme hypothesis: shedding new light on an old disease
    Ana Pamplona
    Unidade de Malaria, Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649 028 Lisboa, Portugal
    Int J Biochem Cell Biol 41:711-6. 2009
    ..In this review, we discuss the role of hemolysis, methemoglobin and free heme in CM, brought to light by our recent studies in mice as well as by other studies ..
  36. ncbi Sickle cell anemia and vascular dysfunction: the nitric oxide connection
    Idowu Akinsheye
    Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Cell Physiol 224:620-5. 2010
    ..Hemoglobin polymerization resulting from the HbS mutation produces erythrocyte deformation and hemolysis. Free hemoglobin, released into plasma by hemolysis scavenges on nitric oxide, and leads to reduced nitric oxide ..
  37. ncbi Storage lesion in banked blood due to hemolysis-dependent disruption of nitric oxide homeostasis
    Mark T Gladwin
    Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Curr Opin Hematol 16:515-23. 2009
    ..However, the degree of intravascular hemolysis and microparticle formation in humans post-transfusion due to both storage and physiological shear has not been ..
  38. pmc Lysine-scanning mutagenesis reveals an amendable face of the cyclotide kalata B1 for the optimization of nematocidal activity
    Yen Hua Huang
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 285:10797-805. 2010
    ..Our data clearly highlight the residues crucial for nematocidal and hemolytic activity in cyclotides, and demonstrate that the nematocidal activity of cyclotides can be increased by incorporation of basic amino acids...
  39. pmc Prevention and treatment of Staphylococcus aureus pneumonia with a beta-cyclodextrin derivative
    Brook E Ragle
    Department of Microbiology, University of Chicago, 920 E 58th St, CLSC 607A, Chicago, IL 60637, USA
    Antimicrob Agents Chemother 54:298-304. 2010
    ..These observations, coupled with a favorable safety profile of beta-cyclodextrin compounds, provide a novel strategy that may be developed to combat S. aureus pneumonia...
  40. ncbi Selective cytotoxicity following Arg-to-Lys substitution in tritrpticin adopting a unique amphipathic turn structure
    Sung Tae Yang
    Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 500 712, South Korea
    FEBS Lett 540:229-33. 2003
    ..Our results obtained in this study may be helpful in the design of drugs that target negatively charged phospholipids...
  41. ncbi Effect of Leucine and Lysine substitution on the antimicrobial activity and evaluation of the mechanism of the HPA3NT3 analog peptide
    Ramamourthy Gopal
    Research Center for Proteineous Materials RCPM, Chosun University, Gwangju, Korea
    J Pept Sci 15:589-94. 2009
    ..The HPA3NT3-analog also induced less hemolysis (4.2%) than the HPA3NT3 peptide (71%) at 200 microM...
  42. ncbi Vesicular stabilization and activity augmentation of enterohaemorrhagic Escherichia coli haemolysin
    Thomas Aldick
    Institute of Hygiene, University of Munster, 48149 Munster, Germany
    Mol Microbiol 71:1496-508. 2009
    ..Our findings provide a general concept for stabilization of RTX toxins and open new insights into the biology of these important virulence factors...
  43. ncbi Enhanced splenomegaly and severe liver inflammation in haptoglobin/hemopexin double-null mice after acute hemolysis
    Emanuela Tolosano
    Department of Genetics, Biology and Biochemistry, University of Turin and the San Giovanni Battista Hospital, Turin, Italy
    Blood 100:4201-8. 2002
    Intravascular hemolysis is associated with several pathologic conditions that include hemoglobinopathies, trauma, malaria, and bacterial infections...
  44. ncbi Human erythrocyte hemolysis induced by selenium and tellurium compounds increased by GSH or glucose: a possible involvement of reactive oxygen species
    Viviane Patrícia P Schiar
    Departamento de Quimica, Centro de Ciencias Naturais e Exatas, Universidade Federal de Santa Maria, 97105 900 Santa Maria, RS, Brazil
    Chem Biol Interact 177:28-33. 2009
    ..stress can induce complex alterations of membrane proteins in red blood cells (RBCs) eventually leading to hemolysis. RBCs represent a good model to investigate the damage induced by oxidizing agents...
  45. ncbi Pore-forming activity of type III system-secreted proteins leads to oncosis of Pseudomonas aeruginosa-infected macrophages
    D Dacheux
    , DBMS, CEA, 17 Avenue des Martyrs, 38054 Grenoble Cedex 09, France
    Mol Microbiol 40:76-85. 2001
    ..Therefore, CHA-induced macrophage death results from a pore-forming activity that is dependent on the intact pcrGVHpopBD operon...
  46. ncbi Haemolysis risk in methylene blue treatment of G6PD-sufficient and G6PD-deficient West-African children with uncomplicated falciparum malaria: a synopsis of four RCTs
    Olaf Müller
    Institute of Public Health, Medical School, Ruprecht Karls University, Heidelberg, Germany
    Pharmacoepidemiol Drug Saf 22:376-85. 2013
    ..Together with a number of other antimalarials, MB is on the list of drugs which potentially induce haemolysis in patients with G6PD deficiency. Ruling out safety concerns is of major importance during drug development...
  47. ncbi Anti-HIV cyclotides from the Chinese medicinal herb Viola yedoensis
    Conan K L Wang
    Institute for Molecular Bioscience, Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Australia
    J Nat Prod 71:47-52. 2008
    ....
  48. pmc Effect of repetitive lysine-tryptophan motifs on the bactericidal activity of antimicrobial peptides
    Ramamourthy Gopal
    Research Center for Proteineous Materials, Chosun University, Kwangju, South Korea
    Amino Acids 44:645-60. 2013
    ..Collectively, the results show (KW)(4) to be the most efficacious peptide in the (KW)( n ) series, exhibiting strong antibacterial activity with little cytotoxicity...
  49. ncbi Loxosceles sphingomyelinase induces complement-dependent dermonecrosis, neutrophil infiltration, and endogenous gelatinase expression
    Denise V Tambourgi
    Laboratório de Imunoquímica, Instituto Butantan, Sao Paulo, Brazil
    J Invest Dermatol 124:725-31. 2005
    ..Furthermore, skin fibroblasts also secreted MMP-9 and MMP-2 upon incubation with venom, suggesting that locally produced MMPs can also contribute to proteolytic tissue destruction...
  50. ncbi Interference in coagulation testing: focus on spurious hemolysis, icterus, and lipemia
    Giuseppe Lippi
    Dipartimento di Patologia e Medicina di Laboratorio, U O Diagnostica Ematochimica, Azienda Ospedaliero Universitaria di Parma, 43100 Parma, Italy
    Semin Thromb Hemost 39:258-66. 2013
    ..In the coagulation laboratory, as well as in other areas of diagnostic testing, spurious hemolysis, icteria, and lipemia in test samples represent by far the leading diagnostic challenges...
  51. pmc Effects of long and short carboxylated or aminated multiwalled carbon nanotubes on blood coagulation
    Jie Meng
    Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People s Republic of China
    PLoS ONE 7:e38995. 2012
    ..The effects of MWCNTs depended on the size and chemistry of the nanotubes and the type of cells they contacted...
  52. ncbi Cell-selective lysis by novel analogues of melittin against human red blood cells and Escherichia coli
    Brijesh K Pandey
    Molecular and Structural Biology Division, Central Drug Research Institute, CSIR, Lucknow 226001, India
    Biochemistry 49:7920-9. 2010
    ....
  53. ncbi Loxosceles intermedia spider envenomation induces activation of an endogenous metalloproteinase, resulting in cleavage of glycophorins from the erythrocyte surface and facilitating complement-mediated lysis
    D V Tambourgi
    Laboratório de Imunoquímica, Instituto Butantan, Sao Paulo, Brazil
    Blood 95:683-91. 2000
    ..most venomous spider in Brazil, and envenomation causes dermonecrosis and complement (C)-dependent intravascular hemolysis. The authors studied the mechanism of induction of C-induced hemolysis...
  54. ncbi Improvement of cyclic decapeptides against plant pathogenic bacteria using a combinatorial chemistry approach
    Sylvie Monroc
    Laboratori d Innovació en Processos i Productes de Síntesi Orgànica LIPPSO, Departament de Quimica, Universitat de Girona, Campus Montilivi, 17071 Girona, Spain
    Peptides 27:2575-84. 2006
    ..The best analogs can be considered as good candidates for the development of effective antibacterial agents for use in plant protection...
  55. ncbi Reversed sequence enhances antimicrobial activity of a synthetic peptide
    Ramamourthy Gopal
    Research Center for Proteineous Materials RCPM, Chosun University, Gwangju, Korea
    J Pept Sci 17:329-34. 2011
    ..Both the peptides show less affinities while binding to EYPC:CH (10:1, w/w). This clearly indicated that the reversal of sequence factors is relevant to increased antimicrobial activity and lipid membrane permeability...
  56. pmc The reality of using primaquine
    Kathy L Burgoine
    Shoklo Malaria Research Unit, Mae Sot, Tak, Thailand, 63110
    Malar J 9:376. 2010
    ..This potentially fatal clinical syndrome can be avoided if patients are tested for G6PD deficiency and adequately informed before being treated...
  57. ncbi Inhibition of oxidative hemolysis by quercetin, but not other antioxidants
    Christopher D Hapner
    Department of Military and Emergency Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Chem Biol Interact 186:275-9. 2010
    ..Quercetin, but not other polyphenols, significantly reduced AAPH-induced oxidative hemolysis. No significant effect on neutrophil oxidative burst or oxidative hemolysis was found with any of the tested ..
  58. ncbi Pulmonary hypertension as a risk factor for death in patients with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    N Engl J Med 350:886-95. 2004
    ..The prevalence of pulmonary hypertension in adults with sickle cell disease, the mechanism of its development, and its prospective prognostic significance are unknown...
  59. ncbi A functional comparison of the venom of three Australian jellyfish--Chironex fleckeri, Chiropsalmus sp., and Carybdea xaymacana--on cytosolic Ca2+, haemolysis and Artemia sp. lethality
    Paul M Bailey
    Department of Biochemistry and Molecular Biology, School of Biomedical and Chemical Sciences, The University of Western Australia, Perth, WA 6009, Australia
    Toxicon 45:233-42. 2005
    ..This suggests that the observed lytic effects may not underlie the lethal effects of the venom, and raises the question of how such potent activities are dealt with by envenomed humans...
  60. ncbi Bacterial two-component and hetero-heptameric pore-forming cytolytic toxins: structures, pore-forming mechanism, and organization of the genes
    Jun Kaneko
    Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
    Biosci Biotechnol Biochem 68:981-1003. 2004
    ..We also discuss the current status of knowledge of the genetic organization of PVL-converting phages in order to achieve an understanding of their molecular evolution...
  61. pmc Cationic amphiphiles, a new generation of antimicrobials inspired by the natural antimicrobial peptide scaffold
    Brandon Findlay
    Department of Chemistry, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada
    Antimicrob Agents Chemother 54:4049-58. 2010
    ..In summary, cationic amphiphiles promise to provide a new and rich source of diverse antibacterial lead structures in the years to come...
  62. pmc Sphingomyelin metabolism at the plasma membrane: implications for bioactive sphingolipids
    Delphine Milhas
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    FEBS Lett 584:1887-94. 2010
    ..We will outline what biological functions are associated with SM metabolism/production at the PM, and discuss what we believe are major challenges that need to be addressed in future studies...
  63. ncbi The effect of oil components on the physicochemical properties and drug delivery of emulsions: tocol emulsion versus lipid emulsion
    Chi Feng Hung
    School of Medicine, Fu Jen Catholic University, Taipei, Taiwan
    Int J Pharm 335:193-202. 2007
    ..The percentages of erythrocyte hemolysis by the emulsions and aqueous micelle system were approximately 0 and approximately 10%, respectively...
  64. pmc Chronic hyper-hemolysis in sickle cell anemia: association of vascular complications and mortality with less frequent vasoocclusive pain
    James G Taylor
    Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 3:e2095. 2008
    Intravascular hemolysis in sickle cell anemia could contribute to complications associated with nitric oxide deficiency, advancing age, and increased mortality...
  65. ncbi Mapping of the complement C9 binding domain in paramyosin of the blood fluke Schistosoma mansoni
    Jiusheng Deng
    Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    Int J Parasitol 37:67-75. 2007
    ..Functional analyses demonstrated that PmyCC inhibited hemolysis of rabbit erythrocytes and of antibody-sensitized sheep erythrocytes by human complement...
  66. ncbi Antitumour activity and specificity as a function of substitutions in the lipophilic sector of helical lactoferrin-derived peptide
    Nannan Yang
    Institute of Medical Biology, Faculty of Medicine, University of Tromsø, Norway
    J Pept Sci 9:300-11. 2003
    ..This information may offer a possibility for de novo design of an antitumour peptide with an improved therapeutic index...
  67. ncbi Enhanced antitumor activity and selectivity of lactoferrin-derived peptides
    N Yang
    Institute of Medical Biology, Faculty of Medicine, University of Tromsø, Norway
    J Pept Res 60:187-97. 2002
    ..Peptides encompassing these parameters were slightly less active against tumor cells, but gained significant selectivity...
  68. ncbi Purification and characterization of a phospholipase A2 isoenzyme isolated from Lachesis muta snake venom
    André L Fuly
    Departamento de Bioquimica Medica, Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro, RJ, Rio de Janeiro, Brazil
    Biochem Pharmacol 63:1589-97. 2002
    ..Pretreatment of LM-PLA2-II with p-bromophenacyl bromide abolished all of these actions, clearly indicating that the biological activities, including the edematogenic effect, are dependent entirely on its enzymatic activity...
  69. ncbi Origin of low mammalian cell toxicity in a class of highly active antimicrobial amphipathic helical peptides
    Ayman Hawrani
    Department of Cellular and Molecular Medicine, Bristol University, Bristol BS8 1TD, United Kingdom
    J Biol Chem 283:18636-45. 2008
    ..We highlight a significant role for tryptophan in promoting binding of amphipathic helical peptides to neutral bilayers, augmenting the arsenal of strategies to reduce mammalian toxicity in antimicrobial peptides...
  70. ncbi A hemolysis-hemagglutination assay for characterizing constitutive innate humoral immunity in wild and domestic birds
    Kevin D Matson
    Department of Biology, University of Missouri, 8001 Natural Bridge Road, St Louis, MO 63121 4499, USA
    Dev Comp Immunol 29:275-86. 2005
    ..8-8.0 titers) vary significantly among species. Accordingly, this assay can be used to compare constitutive innate humoral immunity among species and with respect to age, sex, and experimental treatments within populations...
  71. pmc Direct and synergistic hemolysis caused by Staphylococcus phenol-soluble modulins: implications for diagnosis and pathogenesis
    Gordon Y C Cheung
    Pathogen Molecular Genetics Section, Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, MD 20892, USA
    Microbes Infect 14:380-6. 2012
    ..interest, several PSMs, in particular PSMα peptides, contributed to a considerable extent to synergistic hemolysis with β-toxin or when using the β-toxin-producing strain RN4220 in CAMP assays...
  72. ncbi In vitro hemolysis of human erythrocytes -- by plant extracts with antiplasmodial activity
    P Sharma
    School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India
    J Ethnopharmacol 74:239-43. 2001
    ..been made to relate their antiplasmodial activity with their cytotoxicity as represented by the in vitro rate of hemolysis. Intact erythrocytes were found to respond with a dose--time-integral and fitted to models of pseudo first-order ..
  73. ncbi Toxicity of gold nanoparticles functionalized with cationic and anionic side chains
    Catherine M Goodman
    Department of Chemistry, University of Massachusetts, Amherst 01003, USA
    Bioconjug Chem 15:897-900. 2004
    ..Toxicity, however, has been observed at high concentrations using these systems. MTT, hemolysis, and bacterial viability assays were used to explore differential toxicity among the cell types used, using 2 nm ..
  74. pmc Glomerular hyperfiltration in adult sickle cell anemia: a frequent hemolysis associated feature
    Jean Philippe Haymann
    Universite Pierre et Marie Curie, Universite Paris 6, Institut National de la Santé et de la Recherche Médicale Unit 702, Physiology, Paris, France
    Clin J Am Soc Nephrol 5:756-61. 2010
    ..Glomerular damage is a common feature revealed by a microalbuminuria or a macroalbuminuria. Although glomerular hyperfiltration has been described for decades in this population, its prevalence in young adults is unknown...
  75. pmc Alpha-hemolysin from Escherichia coli uses endogenous amplification through P2X receptor activation to induce hemolysis
    Marianne Skals
    Department of Physiology and Biophysics, Water and Salt Research Center, Aarhus University, Ole Worms Alle 1160, 8000 Aarhus C, Denmark
    Proc Natl Acad Sci U S A 106:4030-5. 2009
    ..Invasive strains frequently produce virulence factors such as alpha-hemolysin (HlyA), which causes hemolysis by forming pores in the erythrocyte membrane...
  76. ncbi Antibacterial and antifungal properties of alpha-helical, cationic peptides in the venom of scorpions from southern Africa
    Leentje Moerman
    Interdisciplinary Research Center, Katholieke Universiteit Leuven Campus Kortrijk, Kortrijk, Belgium
    Eur J Biochem 269:4799-810. 2002
    ..Furthermore, all peptides inhibited growth of fungi. Experiments with SYTOX green suggested that this effect is related to membrane permeabilization...
  77. pmc Imcroporin, a new cationic antimicrobial peptide from the venom of the scorpion Isometrus maculates
    Zhenhuan Zhao
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei, 430072, China
    Antimicrob Agents Chemother 53:3472-7. 2009
    ..These results revealed that imcroporin could be considered a potential anti-infective drug or lead compound, especially for treating antibiotic-resistant pathogens...
  78. pmc MF59-adjuvanted H5N1 vaccine induces immunologic memory and heterotypic antibody responses in non-elderly and elderly adults
    Angelika Banzhoff
    Novartis Vaccines, Marburg, Germany
    PLoS ONE 4:e4384. 2009
    ..Pathogenic avian influenza virus (H5N1) has the potential to cause a major global pandemic in humans. Safe and effective vaccines that induce immunologic memory and broad heterotypic response are needed...
  79. ncbi Suicidal erythrocyte death in sepsis
    Daniela S Kempe
    Department of Physiology, University of Tubingen, Tubingen, Germany
    J Mol Med (Berl) 85:273-81. 2007
    ..Exposure to plasma from septic patients triggers phosphatidylserine exposure leading to adherence to the vascular wall and clearance from circulating blood...
  80. ncbi Ostreopsis sp., a possible origin of palytoxin (PTX) in parrotfish Scarus ovifrons
    Shigeto Taniyama
    Graduate School of Science and Technology, Nagasaki University, Nagasaki 852 8521, Japan
    Toxicon 42:29-33. 2003
    ..in October 1997 at Tokushima Prefecture. From the above results, it can be strongly postulated that the dinoflagellate Ostreopsis sp. is the origin of PTX which is sequestered by the parrotfish S. ovifrons through food chain...
  81. pmc Hemolysis in sickle cell mice causes pulmonary hypertension due to global impairment in nitric oxide bioavailability
    Lewis L Hsu
    Marian Anderson Sickle Cell Center at St Christopher s Hospital for Children, Drexel University College of Medicine, Philadelphia, PA 19134, USA
    Blood 109:3088-98. 2007
    ..the same phenotype, and similar pathobiology was observed in a nonsickle mouse model of acute alloimmune hemolysis. Although the time course is shorter than typical pulmonary hypertension in human sickle cell disease, these ..
  82. ncbi Increased susceptibility in Hp knockout mice during acute hemolysis
    S K Lim
    Cardiovascular Research Institute, National University Medical Institutes, The National University of Singapore
    Blood 92:1870-7. 1998
    ..Induction of severe hemolysis by phenylhydrazine caused extensive hemoglobin precipitation in the renal tubular cells of both -/- and +/+ mice,..
  83. ncbi Chemical inhibition of alpha-toxin, a key corneal virulence factor of Staphylococcus aureus
    Clare C McCormick
    Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA
    Invest Ophthalmol Vis Sci 50:2848-54. 2009
    ..alpha-Toxin mediates extreme corneal damage during Staphylococcus aureus keratitis. Chemical inhibition of this toxin was sought to provide relief from toxin-mediated pathology...
  84. pmc An unusual structural motif of antimicrobial peptides containing end-to-end macrocycle and cystine-knot disulfides
    J P Tam
    Department of Microbiology and Immunology, Vanderbilt University, A 5119 MCN, 1161 21st Avenue South, Nashville, TN 37232 2363, USA
    Proc Natl Acad Sci U S A 96:8913-8. 1999
    ..Furthermore, their end-to-end cyclic structure with a cystine-knot motif represents a molecular structure of antimicrobials and may provide a useful template for the design of novel peptide antibiotics...
  85. ncbi Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease
    Christopher D Reiter
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 8:1383-9. 2002
    ..effects of cell-free, hemoglobin-based blood substitutes have been appreciated, the systemic effects of chronic hemolysis on nitric oxide bioavailability have not been considered or quantified...
  86. ncbi A library of linear undecapeptides with bactericidal activity against phytopathogenic bacteria
    Esther Badosa
    Laboratory of Plant Pathology, Institute of Food and Agricultural Technology CIDSAV CeRTA, University of Girona, Campus Montilivi, 17071 Girona, Spain
    Peptides 28:2276-85. 2007
    ..The peptide H-KKLFKKILKYL-NH(2) (BP100) showed efficacies in flowers of 63-76% at 100 microM, being more potent than BP76 and only less effective than streptomycin, currently used for fire blight control...
  87. ncbi Defective recovery and severe renal damage after acute hemolysis in hemopexin-deficient mice
    E Tolosano
    Department of Genetics, Biology and Biochemistry, University of Turin, Italy
    Blood 94:3906-14. 1999
    ..Moreover, they were sensitive to acute hemolysis, as are wild-type mice...
  88. pmc Erythrocyte glutamine depletion, altered redox environment, and pulmonary hypertension in sickle cell disease
    Claudia R Morris
    Department of Emergency Medicine, Children s Hospital and Research Center Oakland, 747 52nd St, Oakland, CA 94609, USA
    Blood 111:402-10. 2008
    Erythrocyte glutathione depletion has been linked to hemolysis and oxidative stress...
  89. ncbi A pharmacological solution for a conspecific conflict: ROS-mediated territorial aggression in sea anemones
    Grzegorz Bartosz
    Department of Biophysics, University of Lodz, Banacha 12 16, 90237 Lodz, Poland
    Toxicon 51:1038-50. 2008
    ....
  90. ncbi Tumor specific ultrasound enhanced gene transfer in vivo with novel liposomal bubbles
    Ryo Suzuki
    Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, 1091 1 Suwarashi, Sagamiko, Sagamihara, Kanagawa 229 0195, Japan
    J Control Release 125:137-44. 2008
    ..We conclude that the combination of Bubble liposomes and ultrasound is a minimally-invasive and tumor specific gene transfer method in vivo...
  91. pmc RssAB-FlhDC-ShlBA as a major pathogenesis pathway in Serratia marcescens
    Chuan Sheng Lin
    Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kweishan, Taoyuan, Taiwan, Republic of China
    Infect Immun 78:4870-81. 2010
    ..Hemolysin production is essential in this context. Collectively, a major virulence regulatory pathway is identified in S. marcescens...
  92. ncbi Mutagenesis of Propionibacterium acnes and analysis of two CAMP factor knock-out mutants
    Meike Sörensen
    Max Planck Institute for Infection Biology, Department of Molecular Biology, D 10117 Berlin, Germany
    J Microbiol Methods 83:211-6. 2010
    ..acnes. The here presented insertional mutagenesis approach will facilitate future studies on P. acnes...
  93. pmc The hemolytic and cytolytic activities of Serratia marcescens phospholipase A (PhlA) depend on lysophospholipid production by PhlA
    Ken Shimuta
    Department of Bacteriology I, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    BMC Microbiol 9:261. 2009
    ..There have been few studies of the virulence factors of this bacterium. The only S. marcescens hemolytic and cytotoxic factor reported, thus far, is the hemolysin ShlA...
  94. pmc Hemolysis is associated with low reticulocyte production index and predicts blood transfusion in severe malarial anemia
    Rolf Fendel
    Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon
    PLoS ONE 5:e10038. 2010
    ..Emerging drug resistant parasites might aggravate the situation. This case control study investigates biomarkers of enhanced hemolysis in hospitalized children with either SMA or mild malaria (MM).
  95. ncbi Microangiopathic hemolysis and renal failure in malignant hypertension
    Bert Jan H van den Born
    Department of Internal Medicine, Academic Medical Centre, Meibergdreef 9, Room F4 222, PO Box 22660, 1100 DD, Amsterdam, The Netherlands
    Hypertension 45:246-51. 2005
    ..Microangiopathic hemolysis (MAHA) related to malignant hypertension may cause renal insufficiency by obstruction of interlobular arteries...
  96. ncbi Partial characterization of the hemolytic activity of the nematocyst venom from the jellyfish Cyanea nozakii Kishinouye
    Jinhua Feng
    Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
    Toxicol In Vitro 24:1750-6. 2010
    ..PLA(2) activity was also found in the nematocyst venom of C. nozakii. These characteristics provide us a fundamental knowledge in the C. nozakii nematocyst venom which would benefit future research...
  97. pmc Transfusion of human volunteers with older, stored red blood cells produces extravascular hemolysis and circulating non-transferrin-bound iron
    Eldad A Hod
    Departments of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons New York Presbyterian Hospital, New York, NY 10032, USA
    Blood 118:6675-82. 2011
    ..between fresh and older transfusions were only observed in iron parameters and markers of extravascular hemolysis. Compared with fresh RBCs, mean serum total bilirubin increased by 0...
  98. pmc Hemolysis of erythrocytes by granulysin-derived peptides but not by granulysin
    Qing Li
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine, 300 Pasteur Dr, Stanford, CA 94305, USA
    Antimicrob Agents Chemother 49:388-97. 2005
    ..the central region of granulysin recapitulate the lytic activity of the intact molecule, and some peptides cause hemolysis of red blood cells...
  99. ncbi Hemolytic anemia
    Gurpreet Dhaliwal
    San Francisco Veterans Affairs Medical Center University of California San Francisco School of Medicine, San Francisco, California 94121, USA
    Am Fam Physician 69:2599-606. 2004
    b>Hemolysis presents as acute or chronic anemia, reticulocytosis, or jaundice...
  100. ncbi Processing of a 22 kDa precursor protein to produce the circular protein tricyclon A
    Jason P Mulvenna
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    Structure 13:691-701. 2005
    ..The 22 kDa precursor protein of tricyclon A was identified and provides clues to the processing of these fascinating miniproteins...
  101. pmc Biosurfactant production and surface translocation are regulated by PlcR in Bacillus cereus ATCC 14579 under low-nutrient conditions
    Yi Huang Hsueh
    Department of Bacteriology, Food Research Institute, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    Appl Environ Microbiol 73:7225-31. 2007
    ..1722 (m/z). This biosurfactant has hemolytic activity and inhibited the growth of several gram-positive bacteria...

Research Grants69

  1. Iron-mediated vascular disease in sickle cell disease.
    John C Wood; Fiscal Year: 2010
    ..Chronic intravascular hemolysis, which releases red cell components in the circulation, is a leading cause of vasculopathy and acts by impairing ..
  2. Targeting superbugs: discovery and development of new broad-spectrum lipopeptides
    Jian Li; Fiscal Year: 2013
    ..Potential for development of resistance, hemolysis, cytotoxicity and nephrotoxicity, and physicochemical and pharmacokinetic properties, will be assessed...
  3. Mechanisms of Endothelin 1-Induced Sickle Cell Pathology
    Alicia Rivera; Fiscal Year: 2011
    ..It is characterized by red blood cell hemolysis, chronic anemia, and recurrent episodic pain...
  4. Impacts of surface receptors [Toll like receptor (TLRs) and Fc gamma receptors (F
    Collins Ouma; Fiscal Year: 2012
    ..of SMA is multi-factorial and has been attributed to direct destruction of red blood cells (RBCs) by parasites, hemolysis of non-parasitized RBCs, suppression of erythropoiesis, erythrophagocytosis, and dysregulation in cytokine ..
  5. Platelet TSP1 Mediates Vascular Disease and pulmonary hypertension in Sickle Cell
    Enrico M Novelli; Fiscal Year: 2013
    ..mutant hemoglobin S polymerizes when deoxygenated, driving red blood cell (RBC)-dependent vaso-occlusion and hemolysis. These processes lead to platelet and hemostatic activation, pulmonary hypertension and vascular disease...
  6. Clinical and genomic responses to remote ischemic preconditioning
    Brian W McCrindle; Fiscal Year: 2010
    ..in important morbidity and sometimes mortality related to the adverse effects of bypass induced by hypothermia, hemolysis, acid-base management, hemodilution and hypoxia-ischemia...
  7. Non-viral gene therapy for sickle cell anemia
    CLIFFORD JOHN STEER; Fiscal Year: 2012
    ..response of transgenic sickle mice to stem cell gene therapy by examining red cell sickling, Hb expression, hemolysis, oxidative stress, vascular inflammation and vaso-occlusion...
  8. Rheologic and Vascular Modulators in Sickle Vaso-occlusion
    Dhananjay K Kaul; Fiscal Year: 2012
    ..Under Specific Aim 3, we will test the hypothesis that, in SCD, hemolysis and oxidative stress have distinct effects on HIF-1[unreadable] expression and microvascular regulation via NO-..
  9. PlGF-HIF1a-miRNA Axis in Sickle Pulmonary Hypertension
    Punam Malik; Fiscal Year: 2013
    ..The pathogenesis of this vasculopathy is likely multi-factorial, potentiated by hemolysis-induced impaired nitric oxide bioavailability, chronic thrombo-embolism from a procoagulant state, and increased ..
  10. Effect of Diet on Inflammation in Sicke Cell Mice
    Jacqueline M Hibbert; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Characteristic chronic hemolysis in sickle cell anemia is presumed to induce an inflammatory response through sub-clinical vasooclusion...
  11. Heme trafficking and its impact on systemic iron homeostasis
    Janis L Abkowitz; Fiscal Year: 2010
    ..FLVCR into the bile permitting iron to exit the body;and explore the implications of these findings in models of hemolysis, chronic inflammation, and hemochromatosis...
  12. Heme trafficking and its impact on systemic iron homeostasis
    Janis L Abkowitz; Fiscal Year: 2013
    ..FLVCR into the bile permitting iron to exit the body;and explore the implications of these findings in models of hemolysis, chronic inflammation, and hemochromatosis...
  13. Defining target specificity of oxadiazole 2-oxides in Ancylostoma ceylanicum
    Jon J Vermeire; Fiscal Year: 2012
    ..blood, has evolved an efficient mechanism for detoxifying the reactive oxygen species that are generated through hemolysis of red blood cells and protease mediated digestion of hemoglobin...
  14. Randomized trial of inhaled nitric oxide to treat acute pulmonary embolism
    Jeffrey A Kline; Fiscal Year: 2013
    ..b>Hemolysis also disrupts lung NO biosynthesis through the release of arginase-1, which depletes the endothelial nitric ..
  15. Arginine, Nitric Oxide, and Severe Malaria
    JOE BRICE WEINBERG; Fiscal Year: 2012
    ..In vivo NO bioavailability can be decreased because of both reduced NO production and hemolysis (hemoglobin and arginase released from erythrocytes can quench NO and consume arginine, respectively)...
  16. Risk factors for encephalopathy in newborns with severe hyperbilirubinemia
    RICHARD PARTRIDGE WENNBERG; Fiscal Year: 2010
    ..free bilirubin determined by three different methods, photoisomers, and carboxyhemoglobin as an indicator of hemolysis) will be performed in research laboratories at Stanford University...
  17. The role of erythroferrone in iron homeostasis and in iron-loading anemias
    Tomas Ganz; Fiscal Year: 2013
    ..The iron supply to the marrow comes under particular strain after hemorrhage, hemolysis, and other events that trigger expanded erythropoiesis...
  18. Molecular Analysis of the Erythropoietic Stress Response in vivo
    Merav Socolovsky; Fiscal Year: 2010
    ..We will generate Fas and FasL mutant mice on an immune-deficient background free of autoimmune hemolysis, to test the hypothesis that early erythroblast Fas negatively regulates basal erythropoiesis...
  19. Modulators of Nitric Oxide Synthase Activity in Sickle Cell Disease
    Mary E Fabry; Fiscal Year: 2010
    ..We speculate that simultaneous reduction of BH4 and serine phosphorylation will severely impact NOS activity. Hemolysis is thought to be a major factor in reduction of NO bioavailability, both through reaction of cell free ..
  20. Development of a Pediatric Ventricular Assist Device
    Robert Jarvik; Fiscal Year: 2009
    ..Some thrombus, thromboembolism, and hemolysis has occurred, but at an acceptably low incidence...
  21. Alternative Pathway Inhibitors for Orphan Indication
    REKHA BANSAL; Fiscal Year: 2013
    ..to C5 and prevents C5 cleavage and the formation of C5b-9, a complement product responsible for erythrocyte hemolysis. This mechanism prevents intravascular hemolysis (IVH), reduces LDH release, and reduces the need for ..
  22. Cincinnati Center of Excellence in Hemoglobinopathies Research
    Punam Malik; Fiscal Year: 2013
    ..ROS in SCD is derived via RAS-mediated G-protein signaling to worsen oxidative membrane damage and hemolysis using pharmacological, or genetic approaches and study the effects of ROS on cardio-renal pathologies;{Aim 2) ..
  23. Countermeasure for Chlorine Inhalation
    SIGRIDUR G SUMAN; Fiscal Year: 2011
    ..reducing agents based on the known compounds;(3) determine erythrocyte (RBC) cell wall damage using a degree of hemolysis and ektaflow cytometry;and (4) conduct in vitro efficacy studies using an epithelial cell model to evaluate the ..
  24. Heme toxicity and vaso-occlusion in sickle cell disease
    Gregory M Vercellotti; Fiscal Year: 2013
    ..b>Hemolysis releases hemoglobin in the vasculature, which when oxidized to methemoglobin can release toxic heme that ..
  25. Kidney Injury and Dysfunction Support (KIDS)
    Matthew Paden; Fiscal Year: 2010
    ..to an appropriate size (<15% of a patient's calculated blood volume), while maintaining acceptable hemolysis and thrombosis risks...
  26. Hemolytic Anemia: Biochemical, Molecular and Proteomic Diagnostics
    Jeffrey S Friedman; Fiscal Year: 2010
    ..and if there is any relationship between the level of ROS production/oxidative damage and the severity of hemolysis. All samples will undergo standard enzyme studies in parallel so that we may compare proteomics against current ..
  27. Metabolites, Sickle Cell Disease, and Novel Therapeutics
    Yang Xia; Fiscal Year: 2013
    ..induce S1P production within RBCs and that activated platelets and complement activation-induced intravascular hemolysis underlie the further elevation of S1P in plasma...
  28. Ultrasound assisted thrombolysis for acute pulmonary embolism
    CURTIS GENSTLER; Fiscal Year: 2012
    ..v SPECIFIC AIM #2: Optimize blood clot formation and conduct bioefficacy and hemolysis evaluation in vitro...
  29. Effects of Nitric Oxide in Sickle Cell Blood
    Daniel B Kim-Shapiro; Fiscal Year: 2013
    ..Sickle red blood cells are fragile, rupturing during transit in the circulation (hemolysis)...
  30. Development of Therapeutic CD59 inhibitor for treating B-cell malignancies
    Xuebin Qin; Fiscal Year: 2013
    ..NHL cells and primary CLL to RTX or OFA treatment;2) by itself ILYd4 does not adversely mediate in vivo hemolysis of hCD59-expressing erythrocytes;3) the sensitivity to CDC effects mediated by RTX or OFA on RTX-resistant NHL ..
  31. Niacin therapy to improve endothelial function in Sickle Cell Disease
    NAOMI L LUBAN; Fiscal Year: 2011
    ..a mechanism involving consumption of nitric oxide by cell-free plasma hemoglobin released during intravascular hemolysis. We recently have completed studies that directly demonstrate endothelial dysfunction in patients with sickle ..
  32. FLVCR protein trafficking and the regulation of heme export
    JOHN GERARD QUIGLEY; Fiscal Year: 2010
    ..and trafficking may be of relevance to a wide range of human diseases, in particular those characterized by hemolysis or cellular iron overload...
  33. Hemolysis, Lipid Oxidation and Vascular Dysfunction in Sickle Cell Disease
    TIMOTHY CHARLES FLEWELEN; Fiscal Year: 2012
    ..Sickle red cells are more prone to hemolysis than normal red cells, resulting in increased levels of plasma hemoglobin, a known pro-oxidant...
  34. Cellular and Molecular Mechanisms of Acute Lung Injury in Sickle Cell Disease
    SOLOMON FIIFI OFORI-ACQUAH; Fiscal Year: 2013
    ..Our overarching scientific hypothesis is that heme, a product of tissue damage and hemolysis induces ACS via interaction with toll-like receptor 4 (TLR4)...
  35. Pathobiology of Incompatible transfusion
    James C Zimring; Fiscal Year: 2013
    ..Although incompatible transfusion is strictly avoided, because of the risks of hemolysis, hemolysis is not the inevitable outcome of incompatible transfusion...
  36. Ph 2 Study of Vitamin D for Tx of Respiratory Complications in Sickle Cell Ds
    Margaret T Lee; Fiscal Year: 2013
    ..with acute vaso-occlusive complications and chronic multi-organ damage resulting from microvascular oclusion, hemolysis-induced endothelial dysfunction and vasculopathy...
  37. A novel mechanism of coagulation in sickle cell disease
    JULIA BRITTAIN; Fiscal Year: 2012
    ..TF expression/activity and established parameters of coagulation activation in SCD patients in the context of hemolysis and inflammation present in the disease...
  38. Self Regulating Continuous Flow Total Artificial Heart
    Kiyotaka Fukamachi; Fiscal Year: 2013
    ..suction of tissues and atrial pressure imbalance, 3) Perform in vitro system characterization, human blood hemolysis, and endurance testing to verify the CFTAH meets requirements for system performance, resistance to ..
  39. Transfusion-associated immune hemolysis
    Karina Yazdanbakhsh; Fiscal Year: 2010
    ..In our grant application, we will expand our animal studies to focus on the triggers that make immune cells accept or reject transfused blood. ..
  40. Hydroxyurea Pharmacokinetics and Pharmacogenomics
    Russell E Ware; Fiscal Year: 2011
    ..Therapeutic reduction in hemolysis (lower LDH) is also variable though less well described...
  41. Intravenous Magnesium for Sickle Cell Vasoocclusive Crisis
    David C Brousseau; Fiscal Year: 2013
    ..we will gain valuable insight into the pathophysiology of sickle cell crisis through the measurement of hemolysis, nitric oxide pathway metabolites, and markers of cellular injury and inflammation...
  42. Amphotericin B Analogs
    Charles Hutchinson; Fiscal Year: 2009
    ..vitro (MIC values) against a panel of six important fungal pathogens;4) to determine the IC50 for red blood cell hemolysis of the compounds with MIC's =5 micrograms/ml as a surrogate for in vivo nephrotoxicity;and 5) based on all ..
  43. Treatment of sickle cell acute pain episodes with intravenous gammagblobulin
    PATRICIA ANN SHI; Fiscal Year: 2013
    ..to endothelium and red blood cell- leukocyte interactions, microcirculatory blood flow, and serum markers of hemolysis. Completion of this trial, along with further formal training in biostatistics and translational science ..
  44. Characterization of Hemolysin A from Proteus mirabilis
    Todd Weaver; Fiscal Year: 2004
    ..of HpmA (trcHpmA) was found sufficient to activate full-length inactive hemolysin A (HpmA*) and restore in vitro hemolysis. Both a CxxC and a deacetylase motif have been identified within the N-terminal fragment...
  45. Cavopulmonary Assist: Circulatory Support for Fontan Circulation
    Mark D Rodefeld; Fiscal Year: 2013
    ..performance of a rotary disk pump through in vitro feedback from mock flow loop, flow visualization, and hemolysis studies, 4) demonstrate percutaneous viscous pump support in an animal model of univentricular Fontan ..
  46. von Willebrand Factor in Sickle Cell Disease Pathophysiology
    JOSE ARON LOPEZ; Fiscal Year: 2013
    ..of hyperadhesive von Willebrand factor (VWF) in their plasma, the quantity of which correlates with the rate of hemolysis in the patients, b) another study showing that VWF oxidation by neutrophil oxidants increases its platelet ..
  47. PumpKIN PreClinical
    Robert Jarvik; Fiscal Year: 2013
    ..Animal implants have shown that the pump remains free of thrombus and operates at low power without causing hemolysis. The pump represents a completed design, incorporating all of the requirements for a long term implant, ..
  48. PumpKIN Preclinical Program
    Bartley Griffith; Fiscal Year: 2013
    ..Maglev bearingless pump/motor technology, which are ultracompact, and possess a low priming volume and hemolysis risk...
  49. PumpKIN PreClinical
    Robert Jarvik; Fiscal Year: 2012
    ..Animal implants have shown that the pump remains free of thrombus and operates at low power without causing hemolysis. The pump represents a completed design, incorporating all of the requirements for a long term implant, ..
  50. PumpKIN PreClinical
    Robert Jarvik; Fiscal Year: 2010
    ..Animal implants have shown that the pump remains free of thrombus and operates at low power without causing hemolysis. The pump represents a completed design, incorporating all of the requirements for a long term implant, ..
  51. PumpKIN PreClinical
    Robert Jarvik; Fiscal Year: 2011
    ..Animal implants have shown that the pump remains free of thrombus and operates at low power without causing hemolysis. The pump represents a completed design, incorporating all of the requirements for a long term implant, ..
  52. PumpKIN Preclinical Program
    JANET SIMONS; Fiscal Year: 2011
    ..Maglev bearingless pump/motor technology, which are ultracompact, and possess a low priming volume and hemolysis risk...
  53. PumpKIN Preclinical Program
    JANET SIMONS; Fiscal Year: 2010
    ..Maglev bearingless pump/motor technology, which are ultracompact, and possess a low priming volume and hemolysis risk...
  54. PumpKIN Preclinical Program
    Bartley Griffith; Fiscal Year: 2012
    ..Maglev bearingless pump/motor technology, which are ultracompact, and possess a low priming volume and hemolysis risk...
  55. Oxidative Stress & Inflammation in Aging: Insights from Sickle Cell Disease
    JOAN DENISE BECKMAN; Fiscal Year: 2010
    ..Closing this gap in knowledge will be the focus of this proposal. We will examine the link between hemolysis, oxidative stress, vascular inflammation and vaso-occlusion during aging using sickle cell disease as a model...
  56. A Novel Pediatric Pulsatile Rotary Ventricular Pump
    DANIEL EDWARD MAZUR; Fiscal Year: 2010
    ..Performance targets for durability and hemolysis were met...
  57. Synthesis and Evaluation of Nano-antioxidants for Medical Applications
    Choon Lee; Fiscal Year: 2009
    ..diene formation with ultraviolet spectrophotometry while cell damage will be evaluated by red blood cell hemolysis. These studies should provide invaluable data towards the protective effects of nano-antioxidants on ..
  58. NEW METHODOLOGIES FOR THE DESIGN OF SMALL BLOOD PUMPS
    Gerson Rosenberg; Fiscal Year: 2013
    ..and Reynolds stresses, using particle image velocimetry (PIV) and laser Doppler velocimetry (LDV), and in-vitro hemolysis testing...
  59. Labtype to Preclinical Prototype VAD
    William A Smith; Fiscal Year: 2012
    ..Hardware to date has shown good hydraulic performance, low hemolysis, very long life and preliminary in vivo results, compromised by construction shortcuts necessary in the lab ..
  60. Storage Lesion in Banked Blood Due to Disruption of Nitric Oxide Homeostasis
    Daniel B Kim-Shapiro; Fiscal Year: 2013
    ..is due to increased nitric oxide scavenging by cell-free hemoglobin and microparticles that are released during hemolysis in stored blood and diminished nitric oxide production by the newly discovered red cell nitric oxide synthase...
  61. SLS: Molecular Basis and Role in Invasive GAS Disease
    Victor Nizet; Fiscal Year: 2006
    ..GAS are recognized phenotypically by a zone of beta-hemolysis produced largely by the cytolytic toxin streptolysin S (SLS)...
  62. AUTOMATED CLOSED SYSTEM FOR DMSO REMOVAL FROM PERIPHERAL BLOOD STEM CELLS
    ERIK WOODS; Fiscal Year: 2006
    ..skin flushing, headache, fever, dyspnea, abdominal cramping, nausea, and diarrhea to more sever effects such as, hemolysis, cardiovascular symptoms and cerebrovascular ischemia leading to neurological events...
  63. A minimally invasive cardiac assist device
    Walid Aboulhosn; Fiscal Year: 2004
    ..A novel dual-impeller design will allow for lower speed operation, which is expected to reduce hemolysis due to shear gradients...
  64. ROLE OF THE PNH PHENOTYPE IN LEUKEMIC TRANSFORMATION
    Monica Bessler; Fiscal Year: 2005
    ..It presented classically with hemoglobinuria due to intravascular hemolysis, thrombotic complications, and pancytopenia...
  65. REFERENCE LABORATORY TO EVALUATE THERAPIES FOR SCD
    Toshio Asakura; Fiscal Year: 2004
    ..The SCD-RL also studies the adverse effects of potential drugs such as formation of methemoglobin, hemolysis, and/or increase in the amount of denatured hemoglobin inside RBCs...
  66. METHODS FOR PCR SAMPLE PREPARATION--HEME NEUTRALIZATION
    Lily Lin; Fiscal Year: 1991
    ..with low copy number of pathogens since large volumes of blood are required for sample preparation and hemolysis must be avoided in the procedure...
  67. HUMAN PFK ISOZYMES: BIOCHEMICAL AND GENETIC STUDIES
    Alan McLachlan; Fiscal Year: 1993
    ..storage disease type VII [GSD type VII]) is most commonly characterized by exertional myopathy and compensated hemolysis. In patients with this disease, PFK activity is essentially absent from muscle and approximately 50% of the ..
  68. Development of a Miniature Hydrodynamically Suspended Intrathoracic Tesla Pump
    Gerson Rosenberg; Fiscal Year: 2011
    ..designed and tested in vitro and in vivo, demonstrating flows above 12 L/min with 90 mmHg pressure rise and low hemolysis levels. However, the device is large compared to current contemporary LVADs...
  69. Development of a Pediatric Ventricular Assist Device
    Brian Duncan; Fiscal Year: 2009
    ..with minimal host impact, including the potential for minimal or no anticoagulation, low levels of hemolysis and minimal infection risk...