density gradient centrifugation

Summary

Summary: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Top Publications

  1. pmc Isolation and characterization of lipid rafts with different properties from RBL-2H3 (rat basophilic leukaemia) cells
    Galina Radeva
    Department of Chemistry and Biochemistry, University of Guelph, Guelph, ON, Canada N1G 2W1
    Biochem J 380:219-30. 2004
  2. ncbi A comparison of BoviPure and Percoll on bull sperm separation protocols for IVF
    M Samardzija
    Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
    Anim Reprod Sci 91:237-47. 2006
  3. ncbi Comparison of two density gradient centrifugation systems for the enrichment of disseminated tumor cells in blood
    R Rosenberg
    Chirurgische Klinik und Poliklinik, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany
    Cytometry 49:150-8. 2002
  4. ncbi A recombinant particulate antigen of Japanese encephalitis virus produced in stably-transformed cells is an effective noninfectious antigen and subunit immunogen
    A R Hunt
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, PO Box 2087, Fort Collins, CO 80522 2087, USA
    J Virol Methods 97:133-49. 2001
  5. pmc Two distinct size classes of immature and mature subviral particles from tick-borne encephalitis virus
    Steven L Allison
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    J Virol 77:11357-66. 2003
  6. pmc Oligomeric rearrangement of tick-borne encephalitis virus envelope proteins induced by an acidic pH
    S L Allison
    Institute of Virology, University of Vienna, Austria
    J Virol 69:695-700. 1995
  7. ncbi Purification of human respiratory syncytial virus: superiority of sucrose gradient over percoll, renografin, and metrizamide gradients
    A Mbiguino
    Laboratory of Immunovirology, Department of Microbiology and Immunology, University of Montreal, Quebec, Canada
    J Virol Methods 31:161-70. 1991
  8. ncbi 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors
    Paola Grandi
    Cellzome AG, Meyerhofstrasse 1, 69117, Heidelberg, Germany
    Mol Cell 10:105-15. 2002
  9. doi Isolation of lipid droplets from cells by density gradient centrifugation
    Dawn L Brasaemle
    Rutgers, The State University of New Jersey, New Brunswick, New Jersey, USA
    Curr Protoc Cell Biol . 2006
  10. ncbi Localization of the murine Niemann-Pick C1 protein to two distinct intracellular compartments
    W S Garver
    Departments of Cell Biology and Anatomy, University of Arizona, Steele Memorial Children s Research Center, Tucson, Arizona 85724, USA
    J Lipid Res 41:673-87. 2000

Detail Information

Publications203 found, 100 shown here

  1. pmc Isolation and characterization of lipid rafts with different properties from RBL-2H3 (rat basophilic leukaemia) cells
    Galina Radeva
    Department of Chemistry and Biochemistry, University of Guelph, Guelph, ON, Canada N1G 2W1
    Biochem J 380:219-30. 2004
    ....
  2. ncbi A comparison of BoviPure and Percoll on bull sperm separation protocols for IVF
    M Samardzija
    Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
    Anim Reprod Sci 91:237-47. 2006
    ..05). Our results indicate that both protocols gave suitable sperm for IVF. This finding and the similarity between those two density gradient preparations suggests that BoviPure is a good alternative for sperm separation in bovine IVF...
  3. ncbi Comparison of two density gradient centrifugation systems for the enrichment of disseminated tumor cells in blood
    R Rosenberg
    Chirurgische Klinik und Poliklinik, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany
    Cytometry 49:150-8. 2002
    ..Therefore, tumor cell detection calls for enrichment systems with effective depletion of blood cells and high tumor cell recovery...
  4. ncbi A recombinant particulate antigen of Japanese encephalitis virus produced in stably-transformed cells is an effective noninfectious antigen and subunit immunogen
    A R Hunt
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, PO Box 2087, Fort Collins, CO 80522 2087, USA
    J Virol Methods 97:133-49. 2001
    ....
  5. pmc Two distinct size classes of immature and mature subviral particles from tick-borne encephalitis virus
    Steven L Allison
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    J Virol 77:11357-66. 2003
    ..These proteins thus appear to have the intrinsic ability to form alternative assembly products that could provide important clues about the role of lateral envelope protein interactions in flavivirus assembly...
  6. pmc Oligomeric rearrangement of tick-borne encephalitis virus envelope proteins induced by an acidic pH
    S L Allison
    Institute of Virology, University of Vienna, Austria
    J Virol 69:695-700. 1995
    ..This is consistent with a protective role of protein prM for protein E during intracellular transport of immature virions through acidic compartments of the trans-Golgi network...
  7. ncbi Purification of human respiratory syncytial virus: superiority of sucrose gradient over percoll, renografin, and metrizamide gradients
    A Mbiguino
    Laboratory of Immunovirology, Department of Microbiology and Immunology, University of Montreal, Quebec, Canada
    J Virol Methods 31:161-70. 1991
    ..Attempts to purify HRSV over percoll, renografin and metrizamide gradients lead to loss of infectivity. Sucrose was found to represent the best purification medium with a yield of up to 60%...
  8. ncbi 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors
    Paola Grandi
    Cellzome AG, Meyerhofstrasse 1, 69117, Heidelberg, Germany
    Mol Cell 10:105-15. 2002
    ..We conclude that the 40S synthesis machinery predominately associates with the 35S pre-rRNA factors, whereas factors required for 60S subunit synthesis largely bind later, showing an unexpected dichotomy in binding...
  9. doi Isolation of lipid droplets from cells by density gradient centrifugation
    Dawn L Brasaemle
    Rutgers, The State University of New Jersey, New Brunswick, New Jersey, USA
    Curr Protoc Cell Biol . 2006
    ..This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.
  10. ncbi Localization of the murine Niemann-Pick C1 protein to two distinct intracellular compartments
    W S Garver
    Departments of Cell Biology and Anatomy, University of Arizona, Steele Memorial Children s Research Center, Tucson, Arizona 85724, USA
    J Lipid Res 41:673-87. 2000
    ....
  11. pmc Association between hepatitis C virus and very-low-density lipoprotein (VLDL)/LDL analyzed in iodixanol density gradients
    Søren U Nielsen
    Liver Research Group, School of Clinical Medical Sciences, University of Newcastle, England
    J Virol 80:2418-28. 2006
    ..Immunoprecipitation analysis showed that ApoB remained associated with HCV after treatment of serum with deoxycholic acid or NP-40, whereas ApoE was removed from HCV with these detergents...
  12. ncbi Detection of circulating tumor cells in blood using an optimized density gradient centrifugation
    Ralf Gertler
    Chirurgische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universitat Munchen, 81675 Munich, Germany
    Recent Results Cancer Res 162:149-55. 2003
    The aim of the study was to compare the new density gradient centrifugation system OncoQuick with the standard density gradient centrifugation system Ficoll for improved tumor cell enrichment in blood of tumor patients...
  13. ncbi A lipophosphoglycan-independent method for isolation of infective Leishmania metacyclic promastigotes by density gradient centrifugation
    G F Späth
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St, Louis, Missouri, 63110, USA
    Exp Parasitol 99:97-103. 2001
    ..Here we describe a simple density gradient centrifugation method, which allows the rapid purification of infective metacyclic parasites from both wild-type and ..
  14. ncbi Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa
    Shyam S R Allamaneni
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19 1, Cleveland, OH 44195, USA
    Asian J Androl 7:86-92. 2005
    ..To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSperm and ISolate...
  15. ncbi Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates
    Juliana Stevanato
    Department of Surgery, Division of Urology, Sao Paulo Federal University, Sao Paulo, Brazil
    Fertil Steril 90:889-90. 2008
    We wished to verify whether semen processing by discontinuous double-layered density gradient centrifugation could improve sperm apoptotic DNA fragmentation rates using a commercially available deoxynucleotidyl transferase-mediated ..
  16. doi Isolation of endocitic organelles by density gradient centrifugation
    Mariana Eça Guimarães de Araújo
    Methods Mol Biol 424:317-31. 2008
    ..Described methods can be easily modified to isolate other organelles and are compatible with subsequent organelle- and functional organelle proteome analyses by, e.g., two-dimensional gel electrophoresis...
  17. doi Isolation of clathrin-coated vesicles by differential and density gradient centrifugation
    Martine Girard
    Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
    Curr Protoc Cell Biol . 2005
    ..for isolating CCVs from adult rat brain using differential centrifugation, Ficoll and D(2)O-sucrose density gradient centrifugation, and velocity sedimentation in linear sucrose gradients...
  18. ncbi Identification of a 60S preribosomal particle that is closely linked to nuclear export
    J Bassler
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Mol Cell 8:517-29. 2001
    ..Eight of these proteins were copurified with nuclear pore complexes, suggesting that this complex represents the transport intermediate for 60S subunit export...
  19. ncbi Purification of avian circulating primordial germ cells by nycodenz density gradient centrifugation
    D F Zhao
    Department of Materials and Life Science, Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan
    Br Poult Sci 44:30-5. 2003
    ..a method to purify circulating primordial germ cells (cPGCs) in quail and chicks by Nycodenz density gradient centrifugation. 3. The process consisted of primary and secondary purification...
  20. ncbi A novel protocol for the subcellular fractionation of C3A hepatoma cells using sucrose density gradient centrifugation
    Kitambi Satish Srinivas
    Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai 600 020, India
    J Biochem Biophys Methods 60:23-7. 2004
    ..Osmolarity was then restored to the cells and the organelles were separated by density gradient centrifugation. The protein profiles were examined by SDS-PAGE and the purity was analysed by marker enzymes and ..
  21. ncbi Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome
    Evgeny M Makarov
    Department of Cellular Biochemistry, Max Planck Institute of Biophysical Chemistry, , Germany
    Science 298:2205-8. 2002
    ....
  22. ncbi Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles
    R J Sugrue
    Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore
    J Gen Virol 78:1861-6. 1997
    ..The E protein migrated with a buoyant density of 1.13 g/cm3 when analysed using sucrose density gradient centrifugation. Spherical structures with an average diameter of 30 nm, whose morphology resembles dengue virions, ..
  23. pmc Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors
    Leonard J Foster
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Proc Natl Acad Sci U S A 100:5813-8. 2003
    ..Our results provide the first large-scale and unbiased evidence, to our knowledge, for the connection of rafts with signaling and place limits on the fraction of plasma membrane composed by rafts...
  24. ncbi Isolation of mitochondria from rat brain using Percoll density gradient centrifugation
    Neil R Sims
    Centre for Neuroscience and Department of Medical Biochemistry, School of Medicine, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
    Nat Protoc 3:1228-39. 2008
    We have developed procedures that combine differential centrifugation and discontinuous Percoll density gradient centrifugation to isolate mitochondria from rat forebrains and brain subregions...
  25. ncbi Polyclonal anti-colorectal cancer Fab phage display library selected in one round using density gradient centrifugation to separate antigen-bound and free phage
    Brent R Williams
    Department of Pathology and Laboratory Medicine, the Hubert H Humphrey Cancer Research Center, Boston University School of Medicine, Room K707, 715 Albany Street, Boston, MA 02118, USA
    Immunol Lett 81:141-8. 2002
    ..display library was selected for binding to a suspension of the human colorectal cancer cells using density gradient centrifugation, instead of washes, to separate cell-bound and free phage...
  26. ncbi Genome-wide analysis of mRNA polysomal profiles with spotted DNA microarrays
    Daniel Melamed
    Department of Biology, Technion Israel Institute of Technology, Haifa, Israel
    Methods Enzymol 431:177-201. 2007
    ..We then provide a detailed protocol for analysis of polysomal fractions using spotted DNA microarrays...
  27. ncbi Quantification of bacterial subgroups in soil: comparison of DNA extracted directly from soil or from cells previously released by density gradient centrifugation
    S Courtois
    Laboratoire d'Ecologie Microbienne, UMR CNRS 5557, , 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    Environ Microbiol 3:431-9. 2001
    ..However, with the exception of the gamma-subclass of Proteobacteria, there was no significant difference in the spectrum of diversity resulting from the two extraction strategies...
  28. pmc Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction
    Enver Kerem Dirican
    Embryology, MAYA Center for Assisted Reproductive Technologies, Ankara, Turkey
    J Assist Reprod Genet 25:375-81. 2008
    ..We evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected spermatozoa...
  29. ncbi Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception
    M J Tomlinson
    Assisted Conception Unit, Birmingham Women's Hospital, Birmingham, UK
    Hum Reprod 16:2160-5. 2001
    ..Samples were examined before and after preparation using discontinuous density gradient centrifugation. RESULTS: Density gradient centrifugation enriched samples by improving the percentage of ..
  30. ncbi Apoptosis and necrosis in human ejaculated spermatozoa
    Christian Lachaud
    Department of Biochemistry and Molecular Biology, University of Granada, Campus Universitario Fuentenueva, 18701 Granada, Spain
    Hum Reprod 19:607-10. 2004
    ..Features of both apoptosis and necrosis have been reported in ejaculated human spermatozoa. This study examines the relative contribution of these two modes of cell death to the demise of these terminally differentiated cells...
  31. pmc Gastric MUC5AC and MUC6 are large oligomeric mucins that differ in size, glycosylation and tissue distribution
    Henrik Nordman
    Mucosal Biology Group, Department of Cell and Molecular Biology, Level C13, BMC Lund University, S 221 84, Lund, Sweden
    Biochem J 364:191-200. 2002
    ..Following ion-exchange HPLC, both MUC5AC and MUC6 appeared as several distinct populations, probably corresponding to 'glycoforms' of the mucins, the most highly charged of which were found in the gland tissue...
  32. ncbi Comparisons of oocyte maturation times and of three methods of sperm preparation for their effects on the production of goat embryos in vitro
    G J Rho
    College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea
    Theriogenology 56:503-16. 2001
    ..149 +/- 4 and 126 +/- 4, respectively). We conclude that Percoll density-gradient centrifugation is superior to the other two methods for separating goat spermatozoa from frozen-thawed semen in preparation for IVF...
  33. pmc Cell-associated West Nile flavivirus is covered with E+pre-M protein heterodimers which are destroyed and reorganized by proteolytic cleavage during virus release
    G Wengler
    Institut fur Virologie, Justus Liebig Universitat, Giessen, Federal Republic of Germany
    J Virol 63:2521-6. 1989
    ..Preparations of cell-associated virus contained approximately 40,000 physical particles for each PFU...
  34. ncbi Advantage of combining magnetic cell separation with sperm preparation techniques
    Tamer M Said
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19 1, Cleveland, OH 44195, USA
    Reprod Biomed Online 10:740-6. 2005
    ..Semen specimens collected from 15 healthy donors were prepared by either density gradient centrifugation or by one-step sperm wash technique separately and in combination with magnetic cell sorting...
  35. ncbi Integrated morphophysiological assessment of two methods for sperm selection in bovine embryo production in vitro
    A Cesari
    Instituto de Investigaciones Biologicas, Facultad de Ciencias Exactas y Naturales, UNMdP CC 1245 7600 Mar del Plata, Buenos Aires, Argentina
    Theriogenology 66:1185-93. 2006
    ....
  36. pmc Differential biophysical properties of infectious intracellular and secreted hepatitis C virus particles
    Pablo Gastaminza
    The Scripps Research Institute, Maildrop SBR 1010550, North Torrey Pines Road, La Jolla, CA 92037, USA
    J Virol 80:11074-81. 2006
    ..15 to 1.20 g/ml) from extracellular particles (approximately 1.03 to 1.16 g/ml). These results indicate that infectious HCV particles are assembled intracellularly and that their biochemical composition is altered during viral egress...
  37. ncbi Has1p, a member of the DEAD-box family, is required for 40S ribosomal subunit biogenesis in Saccharomyces cerevisiae
    Bertrand Emery
    Departement de Biochimie Medicale, Centre Medical Universitaire, 1, rue Michel Servet, CH 1211 Geneve 4, Switzerland
    Mol Microbiol 52:141-58. 2004
    ..Finally, sequence comparisons suggest that Has1p homologues exist in all eukaryotes, and we show that a has1 null strain can be fully complemented by the Candida albicans homologue...
  38. ncbi Isolation of monocytes from whole blood by density gradient centrifugation and counter-current elutriation followed by cryopreservation: six years' experience
    P K Lund
    Department of Clinical Chemistry, The Research and Development Group, Ullevaal University Hospital, Oslo, Norway
    Scand J Clin Lab Invest 60:357-65. 2000
    ..Monocyte purification by means of counter-current elutriation and subsequent cryopreservation for future use was initiated in 1986 and has been established as a routine since 1993...
  39. ncbi Endotoxin-free purification of monocytes for dendritic cell generation via discontinuous density gradient centrifugation based on diluted Ficoll-Paque Plus
    M Lehner
    Children s Cancer Research Institute, St Anna Children s Hospital, Vienna, Austria
    Int Arch Allergy Immunol 128:73-6. 2002
    ..For the investigation of the biology of human monocyte-derived dendritic cells (DCs), and also for further clinical use of these cells, it is important to separate large numbers of monocytes without isolation-induced activation...
  40. pmc Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method
    Klaus Hartmuth
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 99:16719-24. 2002
    ..These data provide insight into the complexity of the splicing machinery at an early stage of its assembly...
  41. ncbi Lipid rafts of purified mouse brain synaptosomes prepared with or without detergent reveal different lipid and protein domains
    Gunter P Eckert
    Department of Pharmacology, Biocenter Niederursel, University of Frankfurt, Marie Curie Str 9, D 60439, Frankfurt, Germany
    Brain Res 962:144-50. 2003
    ..Lipid rafts from the nerve terminal of neurons prepared with or without detergents may represent different membrane domains each with unique specialized functions...
  42. ncbi Are platelets activated after a rapid, one-step density gradient centrifugation? Evidence from flow cytometric analysis
    K Bagamery
    Department of Anesthesiology, University Hospital of Geneva, Switzerland
    Clin Lab Haematol 27:75-7. 2005
    ..whole blood of healthy donors and pregnancy-induced hypertensive (PIH) patients by a rapid, one-step density gradient centrifugation, and the direct immunofluorescence staining of obtained platelets (CD63)...
  43. pmc Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast
    K A Becherer
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Mol Biol Cell 7:579-94. 1996
    ..These observations suggest that Pep12p may mediate the docking of Golgi-derived transport vesicles at the endosome...
  44. ncbi Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART
    Mona Bungum
    Centre of Reproductive Medicine, Malmo University Hospital, 205 02 Malmo, Sweden
    Hum Reprod 23:4-10. 2008
    ..The aim of this study is to assess whether SCSA analysis of sperm prepared by density gradient centrifugation (DGC) could add more information in regard to the prediction of treatment outcome.
  45. ncbi Molecular detection of breast cancer cells in the peripheral blood of advanced-stage breast cancer patients using multimarker real-time reverse transcription-polymerase chain reaction and a novel porous barrier density gradient centrifugation technology
    Megan K Baker
    Department of Surgery, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Clin Cancer Res 9:4865-71. 2003
    ..Our aim was to make available an assay that was not limited by the low concentration of circulating breast cancer cells and the background gene expression that is typically found in peripheral blood...
  46. ncbi Isolation of Golgi membranes from tissues and cells by differential and density gradient centrifugation
    J M Graham
    Liverpool John Moores University, Liverpool, United Kingdom
    Curr Protoc Cell Biol . 2001
    ..A self-generated iodixinol gradient is described to prepare Golgi membranes from a microsomal fraction of rat hepatocytes, and a standard Golgi enzyme marker assay is given in a support protocol...
  47. ncbi Determining the subunit structure of phosphodiesterases using gel filtration and sucrose density gradient centrifugation
    Wito Richter
    Department of Obstetrics and Gynecology, Stanford University School of Medicine, CA, USA
    Methods Mol Biol 307:167-80. 2005
    ..For this reason, they are used in this study to demonstrate the necessity of performing both gel filtration and density gradient centrifugation to determine the molecular weight of a protein.
  48. ncbi Enveloped particles in the serum of chronic hepatitis C patients
    Marie Anne Petit
    INSERM U271, 151 Cours Albert Thomas, 69424 Lyon Cedex 03, France
    Virology 336:144-53. 2005
    ..The virus was analysed by sucrose density gradient centrifugation. The fractions were tested for viral RNA, core antigen and envelope proteins by using a monoclonal ..
  49. ncbi The neutral comet assay detects double strand DNA damage in selected and unselected human spermatozoa of normospermic donors
    R J Van Kooij
    Division of Perinatology and Gynaecology, University Medical Center, Utrecht, The Netherlands
    Int J Androl 27:140-6. 2004
    ..Ds DNA breaks are a characteristic of the sperm population of normal donors. Current sperm selection procedures reduce the fractions of sperm with ds DNA breaks, yet are not effective in eliminating these cells...
  50. ncbi The preparation of subcellular organelles from mouse liver in self-generated gradients of iodixanol
    J Graham
    Department of Biology, University of Essex, Colchester, United Kingdom
    Anal Biochem 220:367-73. 1994
    ..Because Iodixanol does not interfere significantly with marker enzyme activities, gradient fractions can be analyzed without removal of the gradient medium...
  51. ncbi Purification of fibrillin-containing microfibrils and collagen VI microfibrils by density gradient centrifugation
    C M Kielty
    School of Biological Sciences, University of Manchester, United Kingdom
    Anal Biochem 255:108-12. 1998
    ..from nuchal ligament by bacterial collagenase digestion and size fractionation were purified by CsCl density gradient centrifugation. Localization of collagen VI and fibrillin within the gradient was achieved by SDS-PAGE/Western ..
  52. ncbi Detergent-insoluble glycosphingolipid/cholesterol microdomains of the myelin membrane
    Christopher M Taylor
    Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut 6030 3401, USA
    J Neurochem 81:993-1004. 2002
    ....
  53. ncbi Detecting ribosomal association with the 5' leader of mRNAs by Ribosome Density Mapping (RDM)
    Naama Eldad
    Department of Biology, Technion Israel Institute of Technology, Haifa, Israel
    Methods Enzymol 431:163-75. 2007
    ..In this chapter, we provide a detailed protocol for this procedure and discuss its feasibility...
  54. ncbi An optimized technique for separation of human decidual leukocytes for cellular and molecular analyses
    Olga Nagaeva
    Department of Clinical Immunology, Umea University, Sweden
    Am J Reprod Immunol 47:203-12. 2002
    ..In vitro studies of decidual leukocytes require constant improvement of cell isolation technique in order to obtain highly purified viable and functionally active cells...
  55. ncbi Multiple molecular chaperones complex with misfolded large oligomeric glycoproteins in the endoplasmic reticulum
    G Kuznetsov
    Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 272:3057-63. 1997
    ..We therefore propose that these ER chaperones complex together with a variety of large oligomeric secretory glycoproteins as they fold and assemble in the ER...
  56. ncbi Detection of epithelial cells by RT-PCR targeting CEA, CK20, and TEM-8 in colorectal carcinoma patients using OncoQuick density gradient centrifugation system
    Emmanuel E Lagoudianakis
    First Department of Propaedeutic Surgery, Hippocrateion Hospital, Athens Medical School, Athens, Greece
    J Surg Res 155:183-90. 2009
    ..The detection of epithelial-specific mRNA correlates well with the presence of cancer cells in the peripheral blood and provides a rational explanation for subsequent metastasis...
  57. pmc The raft-promoting property of virion-associated cholesterol, but not the presence of virion-associated Brij 98 rafts, is a determinant of human immunodeficiency virus type 1 infectivity
    Shahan Campbell
    Macfarlane Burnet Institute for Medical Research and Public Health, Cnr Punt and Commercial Roads, Melbourne 3004, Victoria, Australia
    J Virol 78:10556-65. 2004
    ..Our data provide the first direct assessment of virion-associated Brij 98 rafts in retroviral replication and illustrate the importance of the raft-promoting property of virion-associated cholesterol in HIV-1 replication...
  58. ncbi Comparison of sperm separation methods: effect on recovery, motility, motion parameters, and hyperactivation
    G M Centola
    Department of Obstetrics and Gynecology, University of Rochester Medical Center, New York 14642, USA
    Fertil Steril 70:1173-5. 1998
    ....
  59. pmc Clathrin-dependent localization of alpha 1,3 mannosyltransferase to the Golgi complex of Saccharomyces cerevisiae
    T R Graham
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235
    J Cell Biol 127:667-78. 1994
    ..These results indicate that clathrin plays a broader role in the localization of Golgi proteins than anticipated...
  60. ncbi Partial purification and characterization of early and late endosomes from yeast. Identification of four novel proteins
    B Singer-Kruger
    Biocenter, University of Basel, Switzerland
    J Biol Chem 268:14376-86. 1993
    ..of differential centrifugation, flotation on a Nycodenz density gradient, and sedimentation density gradient centrifugation on sucrose/D2O...
  61. ncbi Interaction with type IV pili induces structural changes in the bacterial outer membrane secretin PilQ
    Richard F Collins
    Faculty of Life Sciences, The University of Manchester, Faculty of Life Sciences, Sackville Street, P O Box 88, Manchester M60 1QD, United Kingdom
    J Biol Chem 280:18923-30. 2005
    ..secretin PilQ and the type IV pilus fiber can be detected by far-Western analysis and sucrose density gradient centrifugation. Transmission electron microscopy of preparations of purified pili, to which the purified PilQ ..
  62. ncbi The vesicular acetylcholine transporter interacts with clathrin-associated adaptor complexes AP-1 and AP-2
    Myung Hee Kim
    Department of Molecular and Cellular Biochemistry, University of Kentucky, 800 Rose Street, Lexington, KY 40536, USA
    J Biol Chem 279:12580-7. 2004
    ..There appear to be two endocytosis motifs, one involving the adaptor protein 1 binding site and the other involving the adaptor protein 2 binding site. These results suggest a complex trafficking pathway for VAChT...
  63. pmc Fractionation of the epithelial apical junctional complex: reassessment of protein distributions in different substructures
    Roger Vogelmann
    Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
    Mol Biol Cell 16:701-16. 2005
    ....
  64. pmc Association with membrane protrusions makes ErbB2 an internalization-resistant receptor
    Anette M Hommelgaard
    Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, Copenhagen 2200 N, Denmark
    Mol Biol Cell 15:1557-67. 2004
    ..These data show that ErbB2 is a remarkably internalization-resistant receptor and suggest that the mechanism underlying the firm association of ErbB2 with protrusions also is the reason for this resistance...
  65. ncbi Molecular identification and characterization of Xenopus egg uroplakin III, an egg raft-associated transmembrane protein that is tyrosine-phosphorylated upon fertilization
    Keiichi Sakakibara
    Graduate School of Science and Technology, Kobe University, Nada, Japan
    J Biol Chem 280:15029-37. 2005
    ..Thus, xUPIII represents an egg raft-associated protein that is likely involved in sperm-egg interaction as well as subsequent Src-dependent intracellular events of egg activation in Xenopus...
  66. ncbi Characterization of oligomeric human half-ABC transporter ATP-binding cassette G2
    Junkang Xu
    Department of Pharmacology and Toxicology, Indiana University Cancer Center, Walther Oncology Center Walther Cancer Institute, Indiana University School of Medicine, 1044 W Walnut Street, Indianapolis, IN 46202, USA
    J Biol Chem 279:19781-9. 2004
    ..Further immunoprecipitation analysis showed that the oligomeric ABCG2 did not contain any other proteins. Taken together, we conclude that human ABCG2 likely exists and functions as a homotetramer...
  67. ncbi C18:3-GM1a induces apoptosis in Neuro2a cells: enzymatic remodeling of fatty acyl chains of glycosphingolipids
    Tetsuto Nakagawa
    Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6 10 1, Hakozaki, Higashi ku, Fukuoka 812 8581, Japan
    J Lipid Res 46:1103-12. 2005
    ....
  68. ncbi Identification of a myosin VII-talin complex
    Richard I Tuxworth
    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 280:26557-64. 2005
    ....
  69. ncbi Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7
    Paulo J C Lin
    Department of Biochemistry and Molecular Biology, The University of British Columbia, 2146 Health Sciences Mall, Vancouver, BC V6T 1Z4, Canada
    J Cell Sci 118:1885-97. 2005
    ..We propose a model wherein SCAMPs participate in the shuttling of NHE7 between recycling vesicles and the TGN...
  70. ncbi Pex5p, the peroxisomal cycling receptor, is a monomeric non-globular protein
    João Costa-Rodrigues
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Biol Chem 280:24404-11. 2005
    ..Our results show that Pex5p is a monomeric protein with an abnormal shape. The implications of these findings on current models of protein translocation across the peroxisomal membrane are discussed...
  71. ncbi Cholesterol is required for the polarized secretion of erythropoietin in Madin-Darby canine kidney cells
    Masato Maruyama
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Japan
    Arch Biochem Biophys 438:174-81. 2005
    ..These results indicate that apical secretion of EPO involves a cholesterol-dependent mechanism probably not involving lipid rafts...
  72. pmc A prokaryotic condensin/cohesin-like complex can actively compact chromosomes from a single position on the nucleoid and binds to DNA as a ring-like structure
    A Volkov
    Biochemie, Fachbereich Chemie, Philipps Universität Marburg Max Planck Institut für Terrestrische Mikrobiologie, Marburg, Germany
    Mol Cell Biol 23:5638-50. 2003
    ....
  73. ncbi Biological activities and pore formation of Clostridium perfringens beta toxin in HL 60 cells
    Masahiro Nagahama
    Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro cho, Tokushima 770 8514, Japan
    J Biol Chem 278:36934-41. 2003
    ..We conclude that the toxin acts on HL 60 cells by binding to lipid rafts and forming a functional oligomer with 228 kDa...
  74. ncbi Evidence for a role of SNX16 in regulating traffic between the early and later endosomal compartments
    Brendon J Hanson
    Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Singapore
    J Biol Chem 278:34617-30. 2003
    ..Taken together, these results suggest that SNX16 is a sorting nexin that may function in the trafficking of proteins between the early and late endosomal compartments...
  75. ncbi Folding of active calcium channel beta(1b) -subunit by size-exclusion chromatography and its role on channel function
    Alan Neely
    Centro de Neurociencia de Valparaiso, Universidad de Valparaiso, Valparaíso 2349400, Chile
    J Biol Chem 279:21689-94. 2004
    ....
  76. ncbi Intracellular trafficking of thyroid peroxidase to the cell surface
    Regina Kuliawat
    Division of Endocrinology and Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 280:27713-8. 2005
    ..Rat and mouse thyroid tissue TPO also shows little or no endo H resistance, although cell fractionation still needs to be optimized for these tissues...
  77. ncbi Apolipoprotein A-I-stimulated apolipoprotein E secretion from human macrophages is independent of cholesterol efflux
    Maaike Kockx
    Macrophage Biology Group, Centre for Vascular Research, University of New South Wales, Sydney 2052, Australia
    J Biol Chem 279:25966-77. 2004
    ....
  78. ncbi Mass spectrometry is a powerful tool for identification of proteins associated with lipid rafts of Jurkat T-cell line
    P Pompach
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biochem Soc Trans 32:777-9. 2004
    ..in cold, proteins associated with them could be efficiently purified by techniques such as sucrose-density gradient centrifugation. The complexity of the resulting protein mixture requires powerful MS technique for its analysis...
  79. ncbi Tetraspanin CD82 controls the association of cholesterol-dependent microdomains with the actin cytoskeleton in T lymphocytes: relevance to co-stimulation
    Alix Delaguillaumie
    INSERM U396, Hopital Saint Louis, 1 avenue Claude Vellefaux 75010 Paris, France
    J Cell Sci 117:5269-82. 2004
    ..More generally, the interaction of tetraspanins with raft domains and with the actin cytoskeleton might relate with their role in many cellular functions as membrane organizers...
  80. ncbi Ribophorin I associates with a subset of membrane proteins after their integration at the sec61 translocon
    Cornelia M Wilson
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 280:4195-206. 2005
    ..Alternatively ribophorin I may be multifunctional and facilitate additional processes, for example, ER quality control...
  81. ncbi Secretogranin III binds to cholesterol in the secretory granule membrane as an adapter for chromogranin A
    Masahiro Hosaka
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
    J Biol Chem 279:3627-34. 2004
    ..Thus, we suggest that SgIII directly binds to cholesterol components of the SGM and targets CgA to SGs in pituitary and pancreatic endocrine cells...
  82. ncbi A dynamic pool of calcium in catecholamine storage vesicles. Exploration in living cells by a novel vesicle-targeted chromogranin A-aequorin chimeric photoprotein
    Nitish R Mahapatra
    Department of Medicine, University of California, San Diego, La Jolla 92093 0838, USA
    J Biol Chem 279:51107-21. 2004
    ....
  83. pmc Echovirus 1 endocytosis into caveosomes requires lipid rafts, dynamin II, and signaling events
    Vilja Pietiäinen
    Department of Virology, Haartman Institute, University of Helsinki, FIN 00014 Helsinki, Finland
    Mol Biol Cell 15:4911-25. 2004
    ..These results shed further light on the cell entry of nonenveloped viral pathogens and illustrate the use of viruses as probes to dissect caveolin-associated endocytic pathways...
  84. ncbi Partitioning of NaPi cotransporter in cholesterol-, sphingomyelin-, and glycosphingolipid-enriched membrane domains modulates NaPi protein diffusion, clustering, and activity
    Makoto Inoue
    Department of Medicine, University of Colorado Health Sciences Center and Denver Veterans Affairs Medical Center, Denver, Colorado 80262, USA
    J Biol Chem 279:49160-71. 2004
    ....
  85. ncbi Heat shock induces preferential translation of ERGIC-53 and affects its recycling pathway
    Carmen Spatuzza
    Dipartimento di Biochimica e Biotecnologie Mediche, Universita degli Studi di Napoli Federico II, I 80131, Naples, Italy
    J Biol Chem 279:42535-44. 2004
    ..These results support the view that ERGIC-53 alone, or in association with MCFD2, plays important functions during cellular response to stress conditions...
  86. ncbi Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors
    K Saidas Nair
    Department of Molecular and Cellular Pharmacology and Neuroscience Program, University of Miami, Miami, Florida 33136, USA
    J Biol Chem 279:41240-8. 2004
    ....
  87. pmc Association of active caspase 8 with the mitochondrial membrane during apoptosis: potential roles in cleaving BAP31 and caspase 3 and mediating mitochondrion-endoplasmic reticulum cross talk in etoposide-induced cell death
    Dhyan Chandra
    Dept of Carcinogenesis, University of Texas M D Anderson Cancer Center, Science Park Research Division, Park Rd 1C, Smithville, TX 78957, USA
    Mol Cell Biol 24:6592-607. 2004
    ..Cleavage of BAP31 leads to the generation of ER- localized, proapoptotic BAP20, which may mediate mitochondrion-ER cross talk through a Ca(2+)-dependent mechanism...
  88. ncbi ERK kinase inhibition stabilizes the aryl hydrocarbon receptor: implications for transcriptional activation and protein degradation
    Shujuan Chen
    Laboratory of Environmental Toxicology, Department of Pharmacology, Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 280:4350-9. 2005
    ....
  89. ncbi Lipid rafts in higher plant cells: purification and characterization of Triton X-100-insoluble microdomains from tobacco plasma membrane
    Sebastien Mongrand
    Laboratoire de Biogenese Membranaire, FRE 2694 CNRS Université Victor Segalen Bordeaux 2, 146 rue Leo Saignat, 33076 Bordeaux Cedex, France
    J Biol Chem 279:36277-86. 2004
    ..We demonstrate the recruitment of the NADPH oxidase after elicitation by cryptogein and the presence of the small G protein NtRac5, a negative regulator of NADPH oxidase, in lipid rafts...
  90. ncbi AcrA, AcrB, and TolC of Escherichia coli Form a Stable Intermembrane Multidrug Efflux Complex
    Elena B Tikhonova
    Department of Chemistry and Biochemistry, University of Oklahoma, Norman, 73019, USA
    J Biol Chem 279:32116-24. 2004
    ..We show that antibiotics, the substrates of AcrAB-TolC, stabilize interactions within the complex. However, the formation of the AcrAB-TolC complex does not require an input of energy...
  91. ncbi Lipopolysaccharide transport to the bacterial outer membrane in spheroplasts
    Boris Tefsen
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Biol Chem 280:4504-9. 2005
    ..In contrast to LPS, de novo synthesized phospholipids were not transported to the outer membrane in spheroplasts. Apparently, LPS and phospholipids have different requirements for their transport to the outer membrane...
  92. pmc The deubiquitinating enzyme Ubp1 affects sorting of the ATP-binding cassette-transporter Ste6 in the endocytic pathway
    Carolin Schmitz
    Institut fur Mikrobiologie, Heinrich Heine Universitat Dusseldorf, D 40225 Dusseldorf, Germany
    Mol Biol Cell 16:1319-29. 2005
    ..mUbp1 variant could be localized to the endoplasmic reticulum (ER) membrane by sucrose density gradient centrifugation and by immunofluorescence microscopy...
  93. ncbi Isolation and purification of human placental plasma membranes from normal and pre-eclamptic pregnancies. a comparative study
    V Jimenez
    Programa de Fisiologia y Biofisica, Instituto de Ciencias Biomedicas ICBM, Facultad de Medicina, Universidad de Chile, Casilla 70005, Santiago 7, Chile
    Placenta 25:422-37. 2004
    ..We conclude that MVM and BM isolated from placentae of pre-eclamptic women are similar in enrichment and purity to those of healthy women, thus allowing their use in comparative electrophysiological studies...
  94. ncbi Laminin binding protein, 34/67 laminin receptor, carries stage-specific embryonic antigen-4 epitope defined by monoclonal antibody Raft.2
    Yohko U Katagiri
    Department of Developmental Biology, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo 157 8535, Japan
    Biochem Biophys Res Commun 332:1004-11. 2005
    ..An SSEA-4-positive human EC cell line, NCR-G3 cells, also expressed Raft.2-binding LBP...
  95. ncbi The functional integrity of the serpin domain of C1-inhibitor depends on the unique N-terminal domain, as revealed by a pathological mutant
    Ineke G A Bos
    Department of Immunopathology, Sanquin Research at CLB, and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 278:29463-70. 2003
    ....
  96. ncbi CpG-binding protein (CXXC finger protein 1) is a component of the mammalian Set1 histone H3-Lys4 methyltransferase complex, the analogue of the yeast Set1/COMPASS complex
    Jeong Heon Lee
    Herman B Wells Center for Pediatric Research, Section of Pediatric Hematology Oncology, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    J Biol Chem 280:41725-31. 2005
    ....
  97. ncbi A fast, simple and sensitive method for the detection and quantification of detergent-resistant membranes
    Norbert Blank
    Institute for Clinical Immunology and Rheumatology, University of Erlangen Nuremberg, Glueckstrasse 4a, Germany
    J Immunol Methods 271:25-35. 2002
    ..The presence of protein in these fractions was confirmed by silver-stained polyacrylamide gels. We confirmed the presence of adaptor molecules (LAT) and Src kinases (Lck) in the DRM containing fractions 4 to 6...
  98. ncbi Epithelial growth factor-induced phosphorylation of caveolin 1 at tyrosine 14 stimulates caveolae formation in epithelial cells
    Lidiya Orlichenko
    Center for Basic Research in Digestive Diseases and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 281:4570-9. 2006
    ..This study provides novel insights into how cells regulate caveolae formation and implicates EGF-based signaling cascades in the phosphorylation of Cav1 as a stimulus for caveolae assembly...
  99. ncbi The VP2/VP3 minor capsid protein of simian virus 40 promotes the in vitro assembly of the major capsid protein VP1 into particles
    Masa aki Kawano
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8503, Japan
    J Biol Chem 281:10164-73. 2006
    ..These results are relevant for controlling recombinant capsid formation in vitro, which is potentially useful for the in vitro development of SV40 virus vectors...
  100. ncbi Protein arginine methyltransferase I: substrate specificity and role in hnRNP assembly
    Maciej R Pawlak
    Department of Microbiology and Immunology, Room AA5206 MCN, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2363, USA
    J Cell Biochem 87:394-407. 2002
    ..Finally, methylation of the most abundant Prmt1 substrates appeared to be extensive and constitutive throughout the cell cycle, suggesting the modification does not modulate protein function under normal growth conditions...
  101. pmc Binding of Acf1 to DNA involves a WAC motif and is important for ACF-mediated chromatin assembly
    Dmitry V Fyodorov
    Section of Molecular Biology, University of California, San Diego, La Jolla 92093 0347, USA
    Mol Cell Biol 22:6344-53. 2002
    ....

Research Grants62

  1. PLCgamma regulates HNE3 activity through direct binding and dynamic complexes
    NICHOLAS CONSTANTINE ZACHOS; Fiscal Year: 2011
    ..Biochemical studies, including sucrose density gradient centrifugation, will be performed to analyze the contribution of PLCg on formation of NHE3 complexes...
  2. Analysis of the Spatiotemporal Regulation of Lipolysis
    Lisa M DiPilato; Fiscal Year: 2012
    ..Specifically, I will isolate lipid droplets from 3T3L1 adipocytes via density gradient centrifugation method and probe the fractions for specific isoforms of caveolins and AKAPs upon lipolytic and anti-..
  3. High Efficiency Microfluidic Purification of Stem Cells to Improve Transplants
    James C Sturm; Fiscal Year: 2012
    ..Current techniques, including density gradient centrifugation and differential sedimentation, result in incomplete erythrocyte depletion and may lose 25% ..
  4. Multicenter Cartilage Repair Preclinical Trial in Horses
    Constance R Chu; Fiscal Year: 2010
    ..use the technique of increasing the number of bone marrow derived pluripotential cells through ficoll density gradient centrifugation and culture expansion of what has been classically described as mesenchymal stem cells (MSC)...
  5. Endothelial BK Channel Regulation by Caveolae Targeting
    Hon Chi Lee; Fiscal Year: 2010
    ..These will be assessed by cell fractionation, density gradient centrifugation, immunoprecipitation, immunofluorescence imaging, mutagenesis of hSIo and caveoline-1, and GST-fusion ..
  6. Translational Regulation of Mosquito mRNAs during Plasmodium Infection
    Jinsong Zhu; Fiscal Year: 2010
    ..We will use sucrose density gradient centrifugation to separate mRNAs associated with polyribosomes from those that are translationally inactive...
  7. Examination of the Pre-pubertal Mammalian Growth Plate
    MICHELE REBECCA HUTCHISON; Fiscal Year: 2010
    ..The chondrocytes have been separated by density gradient centrifugation into fractions that correspond to the recognized zones of the growth plate, based on cell size, ..
  8. Biophysical analysis of LRRK2
    DAVID MICHAEL JAMESON; Fiscal Year: 2012
    ..b>Density gradient centrifugation, kinase and GTPase assays, and cell toxicity analyses will be performed in parallel to characterize ..
  9. Perlecan modulates VEGF Bio-Availability in Human Osteoarthritic Cartilage
    RONALD GOMES; Fiscal Year: 2009
    ..Under the first aim, an already proven cesium chloride density gradient centrifugation and anion exchange chromatography approach will be used to purify perlecan from human osteoarthritic ..
  10. VON WILLEBRAND FACTOR, FIBRINOLYSIS, & THE ENDOTHELIUM
    Karen Hamilton; Fiscal Year: 1991
    ..The storage organelle for vWF (Weibel-Palade body) will be purified by colloidal silica density gradient centrifugation. The vWF multimer distribution will be evaluated by immunoblotting of agarose gels and compared to ..
  11. Peripheral Blood Molecular Staging of Breast Cancer
    William E Gillanders; Fiscal Year: 2010
    ..detect breast cancer-associated gene overexpression in the peripheral blood: a novel porous barrier density gradient centrifugation technique, and real-time RT-PCR...
  12. VASOTOCIN RECEPTORS
    PATRICK EGGENA; Fiscal Year: 1990
    ..problem by isolating and purifying AVT receptors from isolated toad bladders by Percoll- and sucrose- density gradient centrifugation, affinity chromatography with a biotinyl derivative of AVT linked to an avidin column, and ..
  13. REGULATION OF PROTEIN TOBACCO MOSAIC VIRUS RNA COMPLEXES
    Vitaly Citovsky; Fiscal Year: 2002
    ..MP-TMV RNA complexes will be isolated from TMV-infected plants by density gradient centrifugation and examined by Western blot analysis, atomic force microscopy, and RNA footprinting...
  14. SERUM PROTEIN BINDING THE HN AUTOANTIGEN
    JOHN KANALAS; Fiscal Year: 1993
    ..monoclonal antibodies to serum reactive proteins using both gel exclusion chromatography and sucrose density gradient centrifugation analysis of sera from diseased rats...
  15. Regulation of Na-CI cotransporter (NCC) subcellular distribution in DCT
    ALICIA MCDONOUGH; Fiscal Year: 2007
    ..Applying a density gradient centrifugation approach discovered that NCC redistributes to higher density membranes in response to high salt diet ..
  16. REGULATION OF PITUITARY HYPERPLASIA AND NEOPLASIA
    Ricardo Lloyd; Fiscal Year: 1999
    ..populations of folliculo-stellate cells using flow cytometric cell sorting and discontinuous percoll density gradient centrifugation. These cells will be characterized by immunohistochemistry, electron microscopy, in situ ..
  17. HISTAMINE METABOLISM IN PULMONARY VASCULAR CELLS
    NANCY BAENZIGER; Fiscal Year: 1992
    ..enzymes will be delineated in subcellular compartments after cell lysis and sucrose or Percoll density gradient centrifugation. Potential binding and cell surface activity of enzymes catalyzing the metabolic steps will be ..
  18. RESPIRATOR EFFICACY VERSUS TUBERCULOSIS AEROSOLS
    John Koerner; Fiscal Year: 2000
    ..Novel applications will be used in the study, such as: density gradient centrifugation for determination of tuberculosis droplet nuclei density; the firefly luciferase assay will be ..
  19. Resolution of Stage-Specific Growth Plate Chondrocytes
    Wenhan Chang; Fiscal Year: 2004
    ..b>Density gradient centrifugation has been used to separate these chondrocytes into subpopulations, but it is unclear that differences ..
  20. SMALL, DENSE LDL IN THE PATHOGENESIS OF PREECLAMPSIA
    Carl Hubel; Fiscal Year: 1999
    ..Thus, Aim 1 is to profile LDL particle size subclasses during gestation and 6 weeks post partum. Density gradient centrifugation is used to isolate LDL from plasma...
  21. Retrograde Signaling in Alcohol-Induced Mitochondrial Stress and Biogenesis.
    Neil Kaplowitz; Fiscal Year: 2009
    ..cytosol) and TORC3 (mitochondria matrix) will be carefully tested defined using differential or density gradient centrifugation, mitoplasts and high resolution confocal imaging: 2) Examine the role of TORC3 in MSR and ..
  22. CHARACTERIZATION OF AH RECEPTOR-RADIOLIGAND INTERACTIONS
    Stephen Safe; Fiscal Year: 1991
    ..be calculated for all the radioligands using a common rat hepatic Ah receptor preparation; b) sucrose density gradient centrifugation studies will be carried out using the selected radioligands (Table D1) and rat hepatic cytosol (..
  23. GLUTATHIONE AND MITOCHONDRIA IN TOXIC RENAL INJURY
    Lawrence Lash; Fiscal Year: 1992
    ..The research will help provide a better understanding of renal drug metabolism and the biochemical mechanisms by which chemicals produce nephrotoxicity...
  24. CAPSULE SYNTHESIS BY MUCOID PSEUDOMONAS AERUGINOSA
    MARILYN STEELE; Fiscal Year: 1992
    ..DNA from mucoid strains of P. aeruginosa will be isolated by cesium chloride density gradient centrifugation, digested with Eco Rl, ligated into lambda gtll, and introduced into E. coli...
  25. BIOCHEMISTRY OF COATED VESICLES
    JAMES ROTHMAN; Fiscal Year: 1999
    ..can be accumulated during cell-free incubations of isolated Golgi membranes, and then purified by density gradient centrifugation following salt extraction...
  26. Probing Microb Comm - Stable Isotope & RNA/DNA Analyses
    James Tiedje; Fiscal Year: 2004
    ..separation of their heavier RNA and DNA from that of the non-active populations (with 12C-RNA/DNA) by density gradient centrifugation. The heavy nucleic acids are then interrogated by (a) sequencing of the ribosomal RNA to determine ..
  27. MECHANISMS OF LUTEAL REGULATION BY GONADOTROPINS
    PATRICK MC ILROY; Fiscal Year: 1992
    ..be made to isolate, using classical techniques such as precipitation, column chromatography, sucrose density gradient centrifugation and electrophoresis, and characterize one or more factors present in urea extracts of luteal ..
  28. FATTY ALCOHOL METABOLISM IN SJOGREN-LARSSON SYNDROME
    WILLIAM RIZZO; Fiscal Year: 1991
    ..These studies will provide fundamental information about normal fatty alcohol metabolism and the deranged fatty alcohol metabolism in SLS...
  29. BETA-ENDORPHIN IN THE PANCREATIC ISLETS
    Rosemary Schleicher; Fiscal Year: 1991
    ..Beta cells will be isolated using Percoll density gradient centrifugation, and a comparison of the relative potencies of a series of opioid agonists on glucose-stimulated ..
  30. SITE AND MECHANISM OF ACTION OF A HEPATOTOXIC PEPTIDE
    THOMAS FOXALL; Fiscal Year: 1990
    ..This potent, naturally occurring, biologically active molecule may eventually be a useful research tool and/or a pharmacologic agent as are other biotoxins...
  31. SEMI-AUTOMATED PRENATAL SCREENING USING MATERNAL BLOOD
    Fatima Merchant; Fiscal Year: 1999
    ..Our approach incorporates fetal cell isolation by routine density gradient centrifugation, followed by uncomplicated May-Giemsa specimen staining...
  32. Actin and myosins in organelle motility
    Christina King Smith; Fiscal Year: 2003
    ..To accomplish this, melanosomes will be purified using density gradient centrifugation, and proteins associated with melanosome membranes will be identified using SDS-PAGE and ..
  33. FECAL COLONOCYTE SCREENING FOR COLORECTAL NEOPLASIA
    David Ahlquist; Fiscal Year: 2000
    ..These markers will be detected by selective immunolabeling of whole fecal colonocytes isolated by density gradient centrifugation. Marker-positive colonocytes will be quantified by immunocytochemistry and flow-cytometry...
  34. HETEROGENEITY OF ALPHA-2 ADRENERGIC RECEPTORS
    David Bylund; Fiscal Year: 1992
    ..radiation inactivation); (4) The apparent molecular size determined by gel filtration and sucrose density gradient centrifugation of receptor preparations solubilized in the presence and absence of agonist; and (5) The apparent ..
  35. OLIGOMERIZATION AND FOLDING OF MEMBRANE PROTEINS
    Rebecca Wells; Fiscal Year: 1999
    ..will be detected by cross-linking mutated proteins made in transfected cells and in vitro, by sucrose-density gradient centrifugation, and by immunofluorescence and co-patching studies on live transfected cells...
  36. CONTROL OF ION TRANSPORT IN HEALTH AND DIARRHEAL DISEASE
    JOHN DOBBINS; Fiscal Year: 1991
    ..and basolateral membranes from these two cell populations using cation precipitation and sucrose density gradient centrifugation techniques...
  37. HIV-LIKE PARTICLES: PRODUCTION AND PURIFICATION SCALE-UP
    STEVEN DISCIULLO; Fiscal Year: 1993
    ..by filtration and/or continuous flow centrifugation and 2) purification by large scale sucrose density gradient centrifugation and/or column chromatography...
  38. LIPIDS AND UROLITHIASIS
    Saeed Khan; Fiscal Year: 1999
    ..Crystal associated vesicles will be separated from the non-crystal associated vesicles by density gradient centrifugation. Lipids of both types of vesicles will be isolated and identified...
  39. MAMMALIAN GENOMES: STASIS AND CHANGE
    HOLLY WICHMAN; Fiscal Year: 1991
    ..Equid DNA will be factionated by density gradient centrifugation, and these fractions will be used to determine whether repetitive families from the Hartmann's ..
  40. CELL MEMBRANE DETERMINANTS OF NAC1 TRANSPORT
    ISIDORE EDELMAN; Fiscal Year: 1980
    ..underway indicate that effective separation of the labeled surfaces is achieved by continuous sucrose density gradient centrifugation. Further explorations of separation methods are planned, including affinity techniques...
  41. RENAL CORTICOSTEROID RECEPTOR STRUCTURE, PURIFICATION
    MERRY SHERMAN; Fiscal Year: 1980
    ..include chromatography on Sephadex LH-20, ion exchange resins and Agarose gels of various porosities, density gradient centrifugation and assays of proteolytic activity using flurorogenic substrates and 125I-fibrin plates...
  42. METHODS FOR PCR SAMPLE PREPARATION: TARGET CAPTURE
    George Cimino; Fiscal Year: 1991
    ..in this manner will be compared to that of target nucleic acids prepared by conventional Ficoll density gradient centrifugation. If the target capture approach is successful, it will significantly simplify sample preparation for ..
  43. EPIDERMAL POLYCYCLIC AROMATIC HYDROCARBON METABOLISM
    John Reiners; Fiscal Year: 1992
    ..differentiating murine keratinocytes, and freshly isolated suspensions of keratinocytes separated by density gradient centrifugation into basal and differentiating cell populations...
  44. INTERACTION OF THERMOTOLERANCE & RRNA TRANSCRIPTION
    John Subjeck; Fiscal Year: 1992
    ..Techniques: In-vivo labeling, polyacrylamide and agar gel electrophoresis, density gradient centrifugation, cell survival, in- vivo/in-vitro tumor systems, cell fractionation, in-vitro transcription systems, ..
  45. REGULATION OF ORGANELLE TRANSPORT IN HUMAN FIBROBLASTS
    John Hayden; Fiscal Year: 1990
    ..b>Density gradient centrifugation and two-dimensional polyacrylamide gel electrophoresis will be used to isolate the particles and ..
  46. CIGARETTE SMOKE AND DNA EXPRESSION IN MAMMALIAN CELLS
    Joseph Wu; Fiscal Year: 1990
    ..These goals will be accomplished by (i) in vivo pulse labeling with radioactive precursors, (ii) density gradient centrifugation, (iii) column chromatography and (iv) determining changes in nucleoid sedimentation...
  47. CELLULAR DIFFERENTIATION OF AIRWAY EPITHELIUM
    MOIRA AITKEN; Fiscal Year: 1991
    ..Epithelial cells will also be fractionated by a density gradient centrifugation procedure and cloned in culture to determine which are stem cells or precursors to other types...
  48. Phosphorylation Dependent Regulation of PTP1B by SUMO Modification
    Shu Chin Yip; Fiscal Year: 2009
    ..between PTP1B sumoylation and its subcellular localization, which will be confirmed biochemically by density gradient centrifugation. PUBLIC HEALTH RELEVANCE Physiological significance of PTP1B sumoylation is accompanied by reduction ..
  49. STEROID HORMONE RECEPTORS IN HUMAN PROSTATE TISSUE
    HERBERT WOTIZ; Fiscal Year: 1980
    ..Determination of specific receptors will be made by sucrose density gradient centrifugation from cytoplasm and nuclei and their presence will be correlated with tissue diagnosis (presence or ..
  50. DEVELOPMENTAL GENETICS OF SPERMATID DIFFERENTIATION
    BARRY KIEFER; Fiscal Year: 1980
    ..The methods of investigation include testes isolation and fractionation, electron microscopy, autoradiography, electrophoresis, density gradient centrifugation, and molecular hybridization.
  51. XALD: Role of Very Long Chain Fatty Acyl-CoA Synthetases
    Paul Watkins; Fiscal Year: 2006
    ..Furthermore, they will facilitate elucidation of the role of enzymes belonging to the VLACS or BG families in XALD. [unreadable] [unreadable]..
  52. Brain Uptake and Utilization of Fatty Acids and Lipids
    Paul Watkins; Fiscal Year: 2004
    ..Mol. Neurosci., which has committed to publish the proceedings of the proposed meeting. ..
  53. Megalin function in the normal and diseased kidney
    DANIEL R BIEMESDERFER; Fiscal Year: 2010
    ..Finally, we will examine the effect of chronic proteinuria on megalin-mediated absorption and signaling by studying these processes in the puromycin aminonucleoside-induced nephrotic rat kidney. ..
  54. HERPESVIRUS PROTEIN SYNTHESIS AND VIRION ASSEMBLY
    D Gibson; Fiscal Year: 2005
    ....
  55. Cocaine Regulation of Norepinephrine Transporter
    LANKUPALLE JAYANTHI; Fiscal Year: 2005
    ..Results from these studies will provide valuable scientific insights to our understanding of the role of drugs of abuse in regulating NET function and expression. ..
  56. ENDOTHELIAL BARRIER FUNCTION IN PREECLAMPSIA
    Yuping Wang; Fiscal Year: 2010
    ..The influence of CLP on placental sFlt-1 will be studied. Results obtained from the proposed work should enhance current knowledge of the role of the placenta and EC dysfunction in the pathogenesis of PE. ..
  57. Proteomic Analysis of Renal Response to Acidosis
    Norman Curthoys; Fiscal Year: 2008
    ..Proximal tubules were isolated by collagenase digestion and Percoll density gradient centrifugation and shown by western blot analysis to be approximately 95% pure...
  58. Leishmania Antigen Presentation by Infected Cells
    PETER KIMA; Fiscal Year: 2007
    ..These studies will expose parasite processes that can be targeted for the control of Leishmania ..
  59. High Throughput Screening for Toll-Like Receptors
    Peter Tobias; Fiscal Year: 2007
    ..This project will utilize an assay that we have recently developed to search for such compounds. [unreadable] [unreadable] [unreadable]..
  60. READTHROUGH ACTIVATION OF GENE TRANSCRIPTION
    David Spector; Fiscal Year: 2001
    ..Understanding new molecular mechanisms for controlling gene expression has applications in the development of viral vectors and cellular expression systems for gene therapy. ..
  61. HIGH THROUGHPUT SCREENING FOR TOLL-LIKE RECEPTORS INHIBITORS
    Peter S Tobias; Fiscal Year: 2010
    ..The goal of this application is to develop techniques for finding new inhibitors of inflammation. ..
  62. Pancreas Transporter Development and Validation
    Michael Taylor; Fiscal Year: 2006
    ..This research program is supported by collaboration with three major clinical centers interested in validation of this perfusion technology for their future use. [unreadable] [unreadable]..