fluorescence spectrometry


Summary: Measurement of the intensity and quality of fluorescence.

Top Publications

  1. ncbi Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
    Nathan C Shaner
    Nat Biotechnol 22:1567-72. 2004
  2. ncbi Fluorescence cross-correlation spectroscopy in living cells
    Kirsten Bacia
    Institute of Biophysics, Dresden University of Technology, Tatzberg 47 51, D 01307 Dresden, Germany
    Nat Methods 3:83-9. 2006
  3. pmc Ultra-high resolution imaging by fluorescence photoactivation localization microscopy
    Samuel T Hess
    Department of Physics and Astronomy, University of Maine, Orono, ME 04469, USA
    Biophys J 91:4258-72. 2006
  4. ncbi A photoactivatable GFP for selective photolabeling of proteins and cells
    George H Patterson
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 297:1873-7. 2002
  5. pmc Mechanisms of tryptophan fluorescence shifts in proteins
    J T Vivian
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    Biophys J 80:2093-109. 2001
  6. ncbi Fluorescence correlation spectroscopy in living cells
    Sally A Kim
    Institute of Biophysics, Dresden University of Technology, Tatzberg 47 51, D 01307 Dresden, Germany
    Nat Methods 4:963-73. 2007
  7. pmc Anomalous diffusion of proteins due to molecular crowding
    Daniel S Banks
    Department of Physics and Astronomy, McMaster University, Hamilton, Ontario, Canada
    Biophys J 89:2960-71. 2005
  8. pmc Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation
    P Schwille
    School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853 USA
    Biophys J 77:2251-65. 1999
  9. ncbi Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots
    Xingyong Wu
    Quantum Dot Corporation, 26118 Research Rd, Hayward, CA 94545, USA
    Nat Biotechnol 21:41-6. 2003
  10. pmc Time-resolved fluorescence of 2-aminopurine as a probe of base flipping in M.HhaI-DNA complexes
    Robert K Neely
    School of Chemistry, The University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK
    Nucleic Acids Res 33:6953-60. 2005

Detail Information

Publications300 found, 100 shown here

  1. ncbi Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
    Nathan C Shaner
    Nat Biotechnol 22:1567-72. 2004
    ..Three monomers with distinguishable hues from yellow-orange to red-orange have higher quantum efficiencies...
  2. ncbi Fluorescence cross-correlation spectroscopy in living cells
    Kirsten Bacia
    Institute of Biophysics, Dresden University of Technology, Tatzberg 47 51, D 01307 Dresden, Germany
    Nat Methods 3:83-9. 2006
    ..We consider FCCS in the context of fluorescence resonance energy transfer and multicolor imaging techniques and discuss application strategies and recent technical advances...
  3. pmc Ultra-high resolution imaging by fluorescence photoactivation localization microscopy
    Samuel T Hess
    Department of Physics and Astronomy, University of Maine, Orono, ME 04469, USA
    Biophys J 91:4258-72. 2006
    ..This new method suggests a means to address a significant number of biological questions that had previously been limited by microscope resolution...
  4. ncbi A photoactivatable GFP for selective photolabeling of proteins and cells
    George H Patterson
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 297:1873-7. 2002
    ..Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange...
  5. pmc Mechanisms of tryptophan fluorescence shifts in proteins
    J T Vivian
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    Biophys J 80:2093-109. 2001
    ..The modeling of electrostatic interactions, including hydration, in proteins is vital to understanding function and structure, and this study helps to assess the effectiveness of current electrostatic models...
  6. ncbi Fluorescence correlation spectroscopy in living cells
    Sally A Kim
    Institute of Biophysics, Dresden University of Technology, Tatzberg 47 51, D 01307 Dresden, Germany
    Nat Methods 4:963-73. 2007
    ..A discussion of advantages and disadvantages of one-photon versus two-photon excitation for FCS is available in Supplementary Methods online...
  7. pmc Anomalous diffusion of proteins due to molecular crowding
    Daniel S Banks
    Department of Physics and Astronomy, McMaster University, Hamilton, Ontario, Canada
    Biophys J 89:2960-71. 2005
  8. pmc Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation
    P Schwille
    School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853 USA
    Biophys J 77:2251-65. 1999
    ..At comparable signal levels, 2PE minimizes photobleaching in spatially restrictive cellular compartments, thereby preserving long-term signal acquisition...
  9. ncbi Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots
    Xingyong Wu
    Quantum Dot Corporation, 26118 Research Rd, Hayward, CA 94545, USA
    Nat Biotechnol 21:41-6. 2003
    ..The results indicate that QD-based probes can be very effective in cellular imaging and offer substantial advantages over organic dyes in multiplex target detection...
  10. pmc Time-resolved fluorescence of 2-aminopurine as a probe of base flipping in M.HhaI-DNA complexes
    Robert K Neely
    School of Chemistry, The University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK
    Nucleic Acids Res 33:6953-60. 2005
    ..Analysis of the AP fluorescence decay function reveals conformational heterogeneity in the DNA-enzyme complexes that cannot be discerned from the present X-ray structures...
  11. ncbi Practical guidelines for dual-color fluorescence cross-correlation spectroscopy
    Kirsten Bacia
    BIOTEC, Department of Biophysics, Dresden University of Technology, Tatzberg 47 51, D 01307 Dresden, Germany
    Nat Protoc 2:2842-56. 2007
    ..It is a technique that is expected to see new applications in various fields of biochemical and cell biological research...
  12. ncbi Detection of quadruplex DNA structures in human telomeres by a fluorescent carbazole derivative
    Cheng Chung Chang
    Institute of Atomic and Molecular Sciences, Academia Sinica, P O Box 23 166, Taipei, 106, Taiwan, Republic of China
    Anal Chem 76:4490-4. 2004
    ..Together these results provide the first evidence for the presence of quadruplex structures in human telomeres...
  13. pmc Photodynamics of red fluorescent proteins studied by fluorescence correlation spectroscopy
    Andreas Schenk
    Department of Biophysics, University of Ulm, Ulm, Germany
    Biophys J 86:384-94. 2004
    ..These observations underscore the intimate connection between protein motions and photophysical processes in fluorescent proteins...
  14. pmc Networks of gold nanoparticles and bacteriophage as biological sensors and cell-targeting agents
    Glauco R Souza
    University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 103:1215-20. 2006
    ..Together, the physical and biological features within these targeted networks offer convenient multifunctional integration within a single entity with potential for nanotechnology-based biomedical applications...
  15. ncbi Structural analysis of human serum albumin complexes with cationic lipids
    David Charbonneau
    Departement de Chimie Biologie, Universite du Quebec a Trois Rivieres, C P 500, Trois Rivieres, Quebec, G9A 5H7, Canada
    J Phys Chem B 113:1777-84. 2009
    ..HSA conformation was stabilized by cholesterol and DOPE with a slight increase of protein alpha-helical structures, while DOTAP and DDAB induced an important (alpha-->beta) transition, suggesting a partial protein unfolding...
  16. ncbi Fluorescent pteridine nucleoside analogs: a window on DNA interactions
    M E Hawkins
    Pediatric Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USA
    Cell Biochem Biophys 34:257-81. 2001
    ..The two guanosine analogs, 3MI and 6MI, and two adenosine analogs, 6MAP and DMAP, are reviewed in detail along with applications utilizing them...
  17. pmc Digital PCR
    B Vogelstein
    The Howard Hughes Medical Institute and The Johns Hopkins Oncology Center, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 96:9236-41. 1999
    ..The process provides a reliable and quantitative measure of the proportion of variant sequences within a DNA sample...
  18. pmc Intercalating nucleic acids containing insertions of 1-O-(1-pyrenylmethyl)glycerol: stabilisation of dsDNA and discrimination of DNA over RNA
    Ulf B Christensen
    Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, DK 5230 Odense M, Denmark
    Nucleic Acids Res 30:4918-25. 2002
    ..Fluorescence measurements show a stronger interaction of the pyrene moiety with DNA than with RNA, indicating intercalation as the stabilising factor in DNA/INA duplexes...
  19. pmc A rapid bioassay for single bacterial cell quantitation using bioconjugated nanoparticles
    Xiaojun Zhao
    Center for Research at the Bio Nano Interface, Department of Chemistry, and The Shands Cancer Center, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 101:15027-32. 2004
    ..The ultimate power of integrating bionanotechnology into complex biological systems will emerge as a revolutionary tool for ultrasensitive detection of disease markers and infectious agents...
  20. ncbi HIV-1 nucleocapsid protein as a nucleic acid chaperone: spectroscopic study of its helix-destabilizing properties, structural binding specificity, and annealing activity
    Maria A Urbaneja
    AIDS Vaccine Program, SAIC Frederick, Building 535 424, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    J Mol Biol 318:749-64. 2002
  21. pmc Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence cross-correlation spectroscopy
    Ping Liu
    Department of Chemistry, National University of Singapore, Singapore
    Biophys J 93:684-98. 2007
    ..These receptors are major targets of anti-cancer drug development, and the receptors' homo- and heterodimeric structures are relevant for such developments...
  22. ncbi How the HIV-1 nucleocapsid protein binds and destabilises the (-)primer binding site during reverse transcription
    Sarah Bourbigot
    Unite de Pharmacologie Chimique et Genetique, CNRS, UMR 8151, INSERM, U 640, Paris, F 75270 cedex 06, France
    J Mol Biol 383:1112-28. 2008
    ..Moreover, the weakening of the upper base pair of the stem likely promotes the melting of the stem that is required to convert the kissing complex into the final (+/-)PBS extended duplex...
  23. ncbi Real-time DNA sequencing from single polymerase molecules
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA
    Science 323:133-8. 2009
    ..Consensus sequences were generated from the single-molecule reads at 15-fold coverage, showing a median accuracy of 99.3%, with no systematic error beyond fluorophore-dependent error rates...
  24. ncbi Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius
    Gert Jan Kremers
    Section Molecular Cytology and Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM, Amsterdam, The Netherlands
    Biochemistry 45:6570-80. 2006
    ..Using the large lifetime difference between SCFP1 and SCFP3A enabled us to perform for the first time dual-lifetime imaging of spectrally identical fluorescent species in living cells...
  25. ncbi Quantum dot bioconjugates for imaging, labelling and sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Nat Mater 4:435-46. 2005
    ..The potential of QDs in biology has just begun to be realized and new avenues will arise as our ability to manipulate these materials improves...
  26. pmc Mapping dynamic protein interactions in MAP kinase signaling using live-cell fluorescence fluctuation spectroscopy and imaging
    Brian D Slaughter
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 104:20320-5. 2007
    ..These results provide a quantitative spatial map of MAPK complexes in vivo and directly support the model that membrane association and regulation of the Ste5 scaffold are critical steps in MAPK activation...
  27. ncbi Biological and chemical applications of fluorescence correlation spectroscopy: a review
    Samuel T Hess
    Department of Physics and School of Applied and Engineering Physics, Clark Hall, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 41:697-705. 2002
  28. pmc Single-molecule and ensemble fluorescence assays for a functionally important conformational change in T7 DNA polymerase
    Guobin Luo
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 104:12610-5. 2007
    ..The high level of selectivity of incoming dNTPs pertinent to this conformational change is a major contributor to replicative fidelity...
  29. pmc Quantification of alpha-synuclein binding to lipid vesicles using fluorescence correlation spectroscopy
    Elizabeth Rhoades
    School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853 2501, USA
    Biophys J 90:4692-700. 2006
    ..Our results demonstrate that fluorescence correlation spectroscopy provides a powerful tool for the quantitative characterization of alphaS-lipid interactions...
  30. ncbi Selenium speciation by high-performance anion-exchange chromatography-post-column UV irradiation coupled with atomic fluorescence spectrometry
    Lina Liang
    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, P O Box 2871, Beijing 100085, China
    J Chromatogr A 1118:139-43. 2006
    ..was established in this paper using high-performance anion-exchange chromatography coupled with atomic fluorescence spectrometry (HPAEC-AFS)...
  31. ncbi Structural characterization and immunogenicity in wild-type and immune tolerant mice of degraded recombinant human interferon alpha2b
    Suzanne Hermeling
    Central Laboratory Animal Institute, Utrecht University, Utrecht, The Netherlands
    Pharm Res 22:1997-2006. 2005
    ..This study was conducted to study the influence of protein structure on the immunogenicity in wild-type and immune tolerant mice of well-characterized degradation products of recombinant human interferon alpha2b (rhIFNalpha2b)...
  32. pmc Probing protein oligomerization in living cells with fluorescence fluctuation spectroscopy
    Yan Chen
    School of Physics and Astronomy, University of Minnesota, 116 Church Street Southeast, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:15492-7. 2003
    ..Resolving protein interactions in a cell is an important step in understanding cellular function on a molecular level. Brightness analysis promises to develop into an important tool for determining protein complex formation in cells...
  33. ncbi Online fluorescent dye detection method for the characterization of immunoglobulin G aggregation by size exclusion chromatography and asymmetrical flow field flow fractionation
    Andrea Hawe
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, 2300 RA Leiden, The Netherlands
    Anal Biochem 378:115-22. 2008
    ..The developed online fluorescent dye detection for HP-SEC or AF4 with Bis-ANS is a powerful method to detect both aggregation and structural changes of both monomeric and aggregated IgG in heat-stressed formulations...
  34. ncbi Attomolar sensitivity in bioassays based on surface plasmon fluorescence spectroscopy
    Fang Yu
    Max Planck Institute for Polymer Research, Ackermannweg 10, D 55128 Mainz, Germany
    J Am Chem Soc 126:8902-3. 2004
    ..Time-resolved ultratrace detection of fluorophore (Alexa-Fluor 647)-labeled rabbit anti-mouse antibody down to 500 aM (10-18 M) was accomplished, corresponding to a binding rate of approximately 10 molecules mm-2 min-1...
  35. ncbi Fluorescence resonance energy transfer as a probe for G-quartet formation by a telomeric repeat
    J L Mergny
    Laboratoire de Biophysique, Museum national d histoire naturelle, INSERM U 201, CNRS UMR 8646, 43 rue Cuvier, 75005 Paris, France
    Chembiochem 2:124-32. 2001
    ..The folding of the G-quartet could be followed at concentrations as low as 100 pM. Fluorescence resonance energy transfer can thus be used to reveal the formation of multistranded DNA structures...
  36. ncbi Catalytic activation of human glucokinase by substrate binding: residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions
    Janne Molnes
    Department of Clinical Medicine, University of Bergen, Norway
    FEBS J 275:2467-81. 2008
  37. pmc Spectroscopic and structural study of proton and halide ion cooperative binding to gfp
    Daniele Arosio
    Scuola Normale Superiore, National Enterprise for nanoScience and nanoTechnology Consiglio Nazionale delle Ricerche Instituto Nazionale di Fisica della Materia, Pisa, Italy
    Biophys J 93:232-44. 2007
    ..halogen complex is formed. The impact of these results on high-sensitivity biosensor design will be discussed...
  38. pmc Sequence-specific binding of human immunodeficiency virus type 1 nucleocapsid protein to short oligonucleotides
    R J Fisher
    Protein Chemistry Laboratory, SAIC Frederick, NCI Frederick Cancer Research and Development Center, Maryland 21702, USA
    J Virol 72:1902-9. 1998
  39. ncbi Examining the proteins of functional retinal lipofuscin using proteomic analysis as a guide for understanding its origin
    Sarah Warburton
    Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA
    Mol Vis 11:1122-34. 2005
    ..To elucidate the origins of biologically active retinal lipofuscin (RLF) by examining its protein composition...
  40. pmc Ethidium derivatives bind to G-quartets, inhibit telomerase and act as fluorescent probes for quadruplexes
    F Koeppel
    Laboratoire de Biophysique, , INSERM U201, CNRS UMR 8646, 43 rue Cuvier, 75005 Paris, France
    Nucleic Acids Res 29:1087-96. 2001
    ..This recognition was associated with telomerase inhibition in vitro: these derivatives showed a potent anti-telomerase activity, with IC(50) values of 18-100 nM in a standard TRAP assay...
  41. ncbi Mechanism of interaction between human serum albumin and N-alkyl phenothiazines studied using spectroscopic methods
    P B Kandagal
    Department of Chemistry, Karnatak University, Dharwad, India
    J Pharm Biomed Anal 47:260-7. 2008
    ..The decreased binding constants of HAS-LMM and HAS-PPC systems in presence of common ions indicated the availability of higher concentration of free drug in plasma...
  42. pmc The kinetics of conformational fluctuations in an unfolded protein measured by fluorescence methods
    Krishnananda Chattopadhyay
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 102:2385-9. 2005
    ..These measurements, which should be widely applicable to other systems, can provide important information about the still incompletely understood conformational properties of unfolded proteins and the mechanism of protein folding...
  43. ncbi A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy
    Kirsten Bacia
    Experimental Biophysics Group, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077, Gottingen, Germany
    Methods 29:74-85. 2003
    ..Practical implications of nonideal conditions including incomplete focus overlap and spectral cross-talk are considered. Recent examples of both auto- and cross-correlation applications demonstrate the potential of FCS for cell biology...
  44. ncbi Two-photon image correlation spectroscopy and image cross-correlation spectroscopy
    P W Wiseman
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093 0339, USA
    J Microsc 200:14-25. 2000
    ..We also demonstrate cellular application of two-photon ICS for measurements of slow diffusion of green fluorescent protein/adhesion receptor constructs within the basal membrane of live CHO fibroblast cells...
  45. pmc Emerging imaging techniques
    Elliot R McVeigh
    Laboratory of Cardiac Energetics, NHLBI, National Institutes of Health, Bethesda, MD, USA
    Circ Res 98:879-86. 2006
    ..The techniques covered are: ultrasound biomicroscopy (UBM), microSPECT, microPET, near infrared imaging, and quantum dots. For each technique, the basic physical principles are explained and recent example applications demonstrated...
  46. pmc Molecular beacons with intrinsically fluorescent nucleotides
    Angel A Marti
    Department of Chemistry, Columbia University, New York, NY 10027, USA
    Nucleic Acids Res 34:e50. 2006
    ..The thermal denaturation profile of MB-FB hybridized with its target shows a marked fluorescence reduction at 53 degrees C, consistent with a transition from double stranded helix to random coil DNA...
  47. pmc Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution
    P Schwille
    Department of Biochemical Kinetics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 72:1878-86. 1997
    ..The concentration of the reaction product can be directly determined from the cross-correlation amplitude...
  48. ncbi Antibody response to aggregated human interferon alpha2b in wild-type and transgenic immune tolerant mice depends on type and level of aggregation
    Suzanne Hermeling
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    J Pharm Sci 95:1084-96. 2006
    ..In conclusion, the transgenic mouse model can be used to screen rhIFNalpha2b formulations for low levels of immunogenic aggregates obtained under accelerated storage conditions...
  49. ncbi Interaction of EGF receptor and grb2 in living cells visualized by fluorescence resonance energy transfer (FRET) microscopy
    A Sorkin
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80111, USA
    Curr Biol 10:1395-8. 2000
    ..The work also highlights the potential of FRET microscopy in the study of subcellular compartmentalization of protein-protein interactions in living cells...
  50. ncbi Unambiguous detection of target DNAs by excimer-monomer switching molecular beacons
    Kazuhisa Fujimoto
    Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama 930 0194, Japan
    J Org Chem 69:3271-5. 2004
    ..Advantages of the excimer-monomer switching molecular beacons were discussed in comparison with conventional ones...
  51. ncbi RNA targeting through binding of small molecules: Studies on t-RNA binding by the cytotoxic protoberberine alkaloid coralyne
    Md Maidul Islam
    Biophysical Chemistry Laboratory, Indian Institute of Chemical Biology Council of Scientific and Industrial Research, 4 Raja S C Mullick Road, Kolkata 700032, India
    Mol Biosyst 5:244-54. 2009
    ..Docking studies provided further insights into the partially intercalated state of coralyne inside the t-RNA structure. This study presents a complete binding and thermodynamic profile of coralyne interaction to t-RNA...
  52. ncbi Promoter clearance by T7 RNA polymerase. Initial bubble collapse and transcript dissociation monitored by base analog fluorescence
    Cuihua Liu
    Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Biol Chem 277:2725-31. 2002
    ..They further indicate that initial bubble collapse precedes dissociation of the 5' end of the RNA...
  53. pmc Enzymatic signal amplification of molecular beacons for sensitive DNA detection
    Jianwei Jeffery Li
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Nucleic Acids Res 36:e36. 2008
    ..Moreover, the extended version provides one additional level of signal amplification, bringing the detection limit down to tens of femtomolar, nearly five orders of magnitude lower than that of conventional hybridization assay...
  54. ncbi Detection and imaging of zinc secretion from pancreatic beta-cells using a new fluorescent zinc indicator
    Kyle R Gee
    Molecular Probes, Inc, 4849 Pitchford Avenue, Eugene, Oregon 97402, USA
    J Am Chem Soc 124:776-8. 2002
    ..Also, within secreting cells some regions of the cell membrane gave rise to secretion while others did not, suggesting active zones of secretion on the cell surface...
  55. ncbi Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs
    Emmanuelle Delagoutte
    Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, Oregon 97403, USA
    J Biol Chem 278:25435-47. 2003
    ..We suggest that changes in the kinetics and thermodynamics of these steps by auxiliary replication proteins constitute a basic mechanism for protein coupling within the replication complex...
  56. ncbi Mechanism of nucleocapsid protein catalyzed structural isomerization of the dimerization initiation site of HIV-1
    Manuela J Rist
    Center for Advanced Research in Biotechnology of the University of Maryland, Rockville 20850, USA
    Biochemistry 41:14762-70. 2002
  57. pmc Probing dynamics of HIV-1 nucleocapsid protein/target hexanucleotide complexes by 2-aminopurine
    S V Avilov
    Institut Gilbert Laustriat, UMR 7175 CNRS Université Louis Pasteur Strasbourg I, Dépt Pharmacologie et Physicochimie, Faculte de Pharmacie, 74 route du Rhin, 67401 Illkirch, France
    Nucleic Acids Res 36:885-96. 2008
    ..Since this platform supports the NC chaperone properties, the restriction of the local mobility of the bases is likely a mechanistic component of these properties...
  58. ncbi Fluorescence resonance energy transfer microscopy: a mini review
    A Periasamy
    University of Virginia, W M Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall, Charlottesville, Virginia 22904, USA
    J Biomed Opt 6:287-91. 2001
    ..This paper will provide a brief review of the above-mentioned FRET techniques...
  59. pmc Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy
    Yan Chen
    Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 82:133-44. 2002
    ..We found the molecular brightness of EGFP to be a very robust parameter, and anticipate the use of PCH analysis for the study of oligomerization processes in vivo...
  60. pmc Spatial-temporal studies of membrane dynamics: scanning fluorescence correlation spectroscopy (SFCS)
    Qiaoqiao Ruan
    Department of Biophysics, University of Illinois in Urbana Champaign, Urbana, IL, USA
    Biophys J 87:1260-7. 2004
    ..Scanning FCS provided a simple, quantitative, yet highly sensitive method to study protein-membrane interactions...
  61. ncbi An ultrasensitive bacterial motor revealed by monitoring signaling proteins in single cells
    P Cluzel
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Science 287:1652-5. 2000
    ..This experimental approach can be extended to quantitative in vivo studies of other biochemical networks...
  62. ncbi Using GFP in FRET-based applications
    B A Pollok
    Aurora Biosciences Corporation, San Diego, CA 92121, USA
    Trends Cell Biol 9:57-60. 1999
    ..This review summarizes the use of GFP for FRET and illustrates the theme with specific examples on how GFP has been employed as an intracellular molecular sensor...
  63. ncbi Interaction of beta-lactoglobulin with resveratrol and its biological implications
    Li Liang
    Chaire de recherche du Canada sur les proteines, les bio systemes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels INAF STELA, Universite Laval, Sainte Foy, Canada
    Biomacromolecules 9:50-6. 2008
    ..Complexing with beta-LG provides a slight increase in the photostability of resveratrol and a significant increase in its hydrosolubility...
  64. ncbi Green fluorescent protein variants as ratiometric dual emission pH sensors. 1. Structural characterization and preliminary application
    George T Hanson
    Department of Chemistry, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Biochemistry 41:15477-88. 2002
    ..Given their favorable optical characteristics, suitable pK(a)'s for the physiological pH range, and suitability for ratiometric measurements, dual emission GFPs should make excellent probes for studying pH in vivo...
  65. pmc Direct measurement of Gag-Gag interaction during retrovirus assembly with FRET and fluorescence correlation spectroscopy
    Daniel R Larson
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 162:1233-44. 2003
    ..These methods also have general applicability to in vivo studies of protein-protein and -membrane interactions involved in the formation of complex macromolecular structures...
  66. pmc Low-energy circular dichroism of 2-aminopurine dinucleotide as a probe of local conformation of DNA and RNA
    Neil P Johnson
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Proc Natl Acad Sci U S A 101:3426-31. 2004
    ..We have used this CD signal to provide information about the conformation of the AP dinucleotide at a defined position within single- and double-stranded nucleic acids...
  67. pmc Conformational instability of the cholera toxin A1 polypeptide
    Abhay H Pande
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL 32826, USA
    J Mol Biol 374:1114-28. 2007
    ..Thermal instability in the reduced CTA1 polypeptide has not been reported before, yet both the translocation and degradation of CTA1 may depend upon this physical property...
  68. ncbi Binding of ricin A-chain to negatively charged phospholipid vesicles leads to protein structural changes and destabilizes the lipid bilayer
    Philip J Day
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U K
    Biochemistry 41:2836-43. 2002
    ..RTA also released trapped calcein from POPG vesicles, indicating that it destabilized the lipid bilayer. We speculate that membrane-induced partial unfolding of RTA during cell entry may facilitate its recognition as an ERAD substrate...
  69. ncbi Flavonoid-serum albumin complexation: determination of binding constants and binding sites by fluorescence spectroscopy
    Claire Dufour
    UMR A408 Institut National de la Recherche Agronomique, Universite d Avignon, Safety and Quality of Plant Products Agroparc, 84914 Avignon Cedex 9, France
    Biochim Biophys Acta 1721:164-73. 2005
    ..Significant differences in affinity and binding location are observed for the highly homologous HSA and BSA...
  70. ncbi Inter- and intramolecular fluorescence quenching of organic dyes by tryptophan
    Nicole Marmé
    Physikalisch Chemisches Institut, Universitat Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany
    Bioconjug Chem 14:1133-9. 2003
    ..Changes in the fluorescence quantum yield of dye-labeled peptides and proteins might be used advantageously for the quantification of proteases and specific binding partners...
  71. ncbi The effect of non-enzymatic glycation on the unfolding of human serum albumin
    Deanna L Mendez
    Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, CA 94132, USA
    Arch Biochem Biophys 444:92-9. 2005
    ..These results showed that glycation altered the local structure around Trp-214 while not significantly impacting the secondary structure, and this alteration translated into an overall change in the stability of gHSA compared to HSA...
  72. ncbi Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors
    Aaron R Clapp
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 126:301-10. 2004
  73. ncbi Characterization of the mangiferin-human serum albumin complex by spectroscopic and molecular modeling approaches
    Yuanyuan Yue
    Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China
    J Pharm Biomed Anal 49:753-9. 2009
    ..Computational mapping of the possible binding sites of mangiferin revealed the molecule to be bound in the large hydrophobic cavity of subdomain IIA...
  74. ncbi Assessment of bile fluorescence patterns in a tropical fish, Nile tilapia (Oreochromis niloticus) exposed to naphthalene, phenanthrene, pyrene and chrysene using fixed wavelength fluorescence and synchronous fluorescence spectrometry
    A Pathiratne
    Department of Zoology, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka
    Bull Environ Contam Toxicol 84:554-8. 2010
    ..This study supports the use of bile fluorescence in Nile tilapia by fixed wavelength fluorescence and synchronous fluorescence spectrometry with non-normalized data as a simple method for screening bioavailability of these PAHs.
  75. pmc High sensitivity of Stark-shift voltage-sensing dyes by one- or two-photon excitation near the red spectral edge
    Bernd Kuhn
    Department for Biomedical Optics, Max Planck Institute for Medical Research, Heidelberg, Germany
    Biophys J 87:631-9. 2004
    ..Such fractional sensitivities are approximately 5 times larger than what is commonly found with other voltage-sensing dyes and approach the theoretical limit given by the spectral Boltzmann tail...
  76. ncbi Structure of the DNA binding domain of E. coli SSB bound to ssDNA
    S Raghunathan
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Campus Box 8231, 660 S Euclid Ave, Saint Louis, Missouri 63110, USA
    Nat Struct Biol 7:648-52. 2000
    ..8 A. This structure describes the vast network of interactions that results in the extensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes...
  77. pmc Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells
    J C Politz
    Worcester Foundation for Biomedical Research, University of Massachusetts Medical Center, Worcester Foundation Campus, Shrewsbury, MA 01545, USA
    Proc Natl Acad Sci U S A 95:6043-8. 1998
    ..These results demonstrate that oligos can move about within the nucleus at rates comparable to those in aqueous solution and further suggest that this is true for large ribonucleoprotein complexes as well...
  78. pmc Analyzing intracellular binding and diffusion with continuous fluorescence photobleaching
    Malte Wachsmuth
    Division Biophysics of Macromolecules, German Cancer Research Center, D 69120 Heidelberg, Germany
    Biophys J 84:3353-63. 2003
    ..The cells show fluorescent nucleoli, and binding is transient. CP yields residence times for mTTF-I-EGFP of approximately 13 s...
  79. ncbi Novel, isotype-specific sensors for protein kinase A subunit interaction based on bioluminescence resonance energy transfer (BRET)
    Anke Prinz
    University of Kassel, Department of Biochemistry, Heinrich Plett Str 40, D 34132 Kassel, Germany
    Cell Signal 18:1616-25. 2006
  80. ncbi DNA cytosine C5 methyltransferase Dnmt1: catalysis-dependent release of allosteric inhibition
    Zeljko M Svedruzić
    Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106, USA
    Biochemistry 44:9472-85. 2005
    ..We describe limitations in the Michaelis-Menten kinetic analysis of Dnmt1 and suggest alternative approaches...
  81. ncbi Temperature-dependent simultaneous ligand binding in human serum albumin
    Sudarson Sekhar Sinha
    Unit for Nano Science and Technology, Department of Chemical, Biological and Macromolecular Sciences, SN Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India
    J Phys Chem B 112:4884-91. 2008
    ..From the FRET studies, an attempt has been made to characterize the simultaneous binding of the two ligands in various temperature-dependent folded states of HSA...
  82. pmc The photon counting histogram in fluorescence fluctuation spectroscopy
    Y Chen
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 77:553-67. 1999
  83. ncbi Simple fluorescent pyrimidine analogues detect the presence of DNA abasic sites
    Nicholas J Greco
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093, USA
    J Am Chem Soc 127:10784-5. 2005
    ..The most responsive of the family was incorporated in DNA. This isosteric fluorescent DNA analogue monitors denaturation of a DNA duplex via fluorescence and positively detects the presence of abasic sites in DNA duplexes...
  84. ncbi Cold vapour atomic fluorescence spectrometry and gas chromatography-pyrolysis-atomic fluorescence spectrometry for routine determination of total and organometallic mercury in food samples
    Les Ebdon
    Plymouth Environmental Research Centre, University of Plymouth, Drake Circus, Plymouth
    Analyst 127:1108-14. 2002
    ..after nitric acid/peroxide digestion, evaporation of the solvent and detection by cold vapour-atomic fluorescence spectrometry. Full organomercury speciation requires a clean-up step before analysis of the final extract in ..
  85. ncbi A close look at fluorescence quenching of organic dyes by tryptophan
    Soren Doose
    Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Universitatsstr 25, 33615 Bielefeld, Germany
    Chemphyschem 6:2277-85. 2005
  86. ncbi Peculiar 2-aminopurine fluorescence monitors the dynamics of open complex formation by bacteriophage T7 RNA polymerase
    R P Bandwar
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Biol Chem 276:14075-82. 2001
    ..Thus, in the absence of the initiating nucleotide, GTP, the overall equilibrium constant for closed to open complex conversion is 0.5 and the net rate of open complex formation is nearly 150 s(-1)...
  87. ncbi Whole-body fluorescence lifetime imaging of a tumor-targeted near-infrared molecular probe in mice
    Sharon Bloch
    Washington University School of Medicine, Department of Radiology, St Louis, Missouri 63110, USA
    J Biomed Opt 10:054003. 2005
    ..This study clearly demonstrated the feasibility of whole-body NIR fluorescence lifetime imaging for tumor localization and its spatial functional status in living small animals...
  88. ncbi The LSM 510 META - ConfoCor 2 system: an integrated imaging and spectroscopic platform for single-molecule detection
    Klaus Weisshart
    Carl Zeiss Jena GmbH, Carl Zeiss Promenade 10, 07745 Jena, Germany
    Curr Pharm Biotechnol 5:135-54. 2004
    ..In addition, we outline the calibration of the instrument and the work flow for data analysis with emphasis on in vivo pharmacological applications...
  89. ncbi Simultaneous speciation analysis of Sb(III), Sb(V) and (CH3)3SbCl2 by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry detection (HPLC-HG-AFS): application to antimony speciation in sea water
    Ida De Gregori
    Laboratorio de Química Analítica y Ambiental, Instituto de Quimica, Pontificia Universidad Catolica de Valparaiso, Avenida Brasil 2950, P O Box 4059, Valparaiso, Chile
    J Chromatogr A 1091:94-101. 2005
    ..species by high performance liquid chromatography (HPLC) and its detection by hydride generation-atomic fluorescence spectrometry (HG-AFS)...
  90. ncbi Pyrene-labeled oligodeoxynucleotide probe for detecting base insertion by excimer fluorescence emission
    Akimitsu Okamoto
    Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University and SORST, Japan Science and Technology Corporation, Kyoto 615 8510, Japan
    J Am Chem Soc 126:8364-5. 2004
    ..This method is useful for the determination of the presence/absence of insertion polymorphisms located at a specific site on the target DNA...
  91. ncbi Simultaneous determination of levodopa and carbidopa by synchronous fluorescence spectrometry using double scans
    Wook Hyun Kim
    Department of Chemistry, Kyungpook National University, Taegu 702 701, Republic of Korea
    Anal Chim Acta 619:2-7. 2008
    ..006 microg mL(-1) for carbidopa and levodopa, respectively. The method was successfully applied to the determination of levodopa and carbidopa in pharmaceutical formulation and urine sample. The recovery results were satisfactory...
  92. ncbi Indirect determination of sulfide at ultratrace levels in natural waters by flow injection on-line sorption in a knotted reactor coupled with hydride generation atomic fluorescence spectrometry
    Yan Jin
    School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry and Key Laboratory of MOE for Life Science, Nanjing University, Nanjing 210093, China
    Anal Chem 79:7176-81. 2007
    ..reactor (KR) was developed for flow injection on-line sorption coupled with hydride generation atomic fluorescence spectrometry (HG-AFS). With the Hg2+ pH kept at 2...
  93. ncbi Kinetic stabilization of the alpha-synuclein protofibril by a dopamine-alpha-synuclein adduct
    K A Conway
    Center for Neurologic Diseases, Brigham and Women s Hospital, Department of Neurology, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA
    Science 294:1346-9. 2001
    ..Adduct formation provides an explanation for the dopaminergic selectivity of alpha-synuclein-associated neurotoxicity in PD and has implications for current and future PD therapeutic and diagnostic strategies...
  94. ncbi Fluorescence spectroscopy of single biomolecules
    S Weiss
    Materials Sciences and Physical Biosciences Divisions, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Science 283:1676-83. 1999
    ..The progress in applying these tools to biological studies with the use of fluorophores that are site-specifically attached to macromolecules is reviewed...
  95. ncbi In vivo cancer targeting and imaging with semiconductor quantum dots
    Xiaohu Gao
    Department of Biomedical Engineering, and the Winship Cancer Institute, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001, Atlanta, Georgia 30322, USA
    Nat Biotechnol 22:969-76. 2004
    ..These results raise new possibilities for ultrasensitive and multiplexed imaging of molecular targets in vivo...
  96. pmc EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion
    Jörg Wiedenmann
    Department of General Zoology and Endocrinology, University of Ulm, 89069 Ulm, Germany
    Proc Natl Acad Sci U S A 101:15905-10. 2004
    ..The possibility to locally change the emission wavelength by focused UV light makes EosFP a superb marker for experiments aimed at tracking the movements of biomolecules within the living cell...
  97. ncbi Oligomeric assembly of native-like precursors precedes amyloid formation by beta-2 microglobulin
    Catherine M Eakin
    Department of Molecular Biophysics and Biochemistry, Yale University, 260 Whitney Avenue, New Haven, Connecticut 06520 8114, USA
    Biochemistry 43:7808-15. 2004
    ..The native-like structure and discrete oligomeric size of beta2m amyloid intermediates suggest that this protein forms fibrils by structural domain swapping...
  98. ncbi Speciation analysis of mercury in sediments, zoobenthos and river water samples by high-performance liquid chromatography hyphenated to atomic fluorescence spectrometry following preconcentration by solid phase extraction
    Jana Margetínová
    Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, Brno CZ 613 00, Czech Republic
    Anal Chim Acta 615:115-23. 2008
    ..The limits of detection were 4.3microg L(-1) for methylmercury (MeHg(+)), 1.4microg L(-1) for ethylmercury (EtHg(+)), 0.8microg L(-1) for inorganic mercury (Hg(2+)), 0.8microg L(-1) for phenylmercury (PhHg(+))...
  99. pmc Probing polyproline structure and dynamics by photoinduced electron transfer provides evidence for deviations from a regular polyproline type II helix
    Soren Doose
    Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Proc Natl Acad Sci U S A 104:17400-5. 2007
  100. ncbi Direct determination of closely overlapping drug mixtures of diflunisal and salicylic acid in serum by means of derivative matrix isopotential synchronous fluorescence spectrometry
    J A Murillo Pulgarin
    Department of Analytical Chemistry and Foods Technology, University Castilla La Mancha, 13071 Ciudad Real, Spain
    Anal Chim Acta 583:55-62. 2007
    ..The limit of detection calculated according to Clayton who uses error propagation throughout the calibration curve and a non-centralized security factor was 36.8 and 37.3 ng mL(-1) for diflunisal and salicylic acid, respectively...
  101. pmc Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2
    Yvonne N Tallini
    Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA
    Proc Natl Acad Sci U S A 103:4753-8. 2006
    ..d. 13.5, atrioventricular conduction through the canal was abolished at this stage. These studies demonstrate that GCaMP2 will have broad utility in the dissection of numerous complex cellular interactions in mammals, in vivo...

Research Grants40

    DANYEL TACKER; Fiscal Year: 2004
    ..be treated with CE and intracellular cation flux (calcium, magnesium, and hydronium) recorded utilizing fluorescence spectrometry. Flow cytometry and/or Western blotting will be used to monitor changes in calcium channel density over ..
  2. Optimized Hydride Generation Systems for Arsenic Specia*
    Miroslav Styblo; Fiscal Year: 2006
    ..and instrumentation to increase sensitivity of the arsenic speciation analyses by AAS or by atomic fluorescence spectrometry. The optimized HG systems will then be used in the parent project for analysis of metabolites produced ..
    RODNEY WELCH; Fiscal Year: 2001
    ..These methods involve CD spectroscopy, mass spectrometry, tryptophan fluorescence spectrometry, FACS analysis of hemolysin-MAb reactivities, and site-specific-fluorescein-labeled hemolysin...
    JAMES WINEFORDNER; Fiscal Year: 1991
    ..of trace elements in small amounts of biological samples and their use as sources in atomic fluorescence spectrometry; (7) development of a laser microanalyzer to allow spatial measurements of small amounts of non-..
    SANDRA GREER; Fiscal Year: 2001
    ..mass density by dilatometry, the enthalpy by stopped flow calorimetry, the extent of polymerization by fluorescence spectrometry, and the concentration susceptibility and length scales by neutron scattering, all as functions of the ..
    Michael Barry; Fiscal Year: 2003
    ..mass spectrometry to identify metabolites implicated in known syndromes of bone marrow failure; and fluorescence spectrometry for the detection of oxidative stress...
  7. Nuclease-Resistant Aptamers for Anthrax Opsonization
    John Bruno; Fiscal Year: 2004
    ..Such shielded aptamers could even be used in inhalers to enhance alveolar macrophage phagocytosis. ..
  8. Nuclease-Resistant Aptamers to Botox and Anthrax Toxins
    John Bruno; Fiscal Year: 2004
    ..Such reagents could also be used in environmental biosensors or diagnostic test kits for detection of BOTOX and anthrax toxins in body fluids or environmental samples. ..
  9. MOEMS device for fluorescence spectroscopy of tissues and cells
    Laura Marcu; Fiscal Year: 2006
    ..The device will widely be applied to various biomedical instruments that require wavelength differentiation and selection. ..
  10. Fluorescence lifetime method for guided therapy of brain tumors
    Laura Marcu; Fiscal Year: 2007
    ..While this project is initially tailored to brain tumors demarcation, the device could potentially be also applied to diagnosis of other tumor types. [unreadable] [unreadable] [unreadable]..
  11. Technology for DNA Damage Analysis and Biomarkers
    X Le; Fiscal Year: 2004
    ..The technology will also be useful for monitoring DNA damage induced by anticancer agents and for studying DNA repair, which is an essential protection mechanism against cancer initiation. ..
  12. Interactions in the Site of Acetylcholinesterase
    Terrone Rosenberry; Fiscal Year: 2005
    ..abstract_text> ..
  13. Semiconductor Nanocrystals for Biomedical Imaging
    Fei Wang; Fiscal Year: 2005
    ..Both types of encapsulated nanoparticle probes will be used to transfect cultured mammalian cells in order to assess QD luminescence in a physiological milieu. ..
    Henry J Pownall; Fiscal Year: 2010
    Carlos Bustamante; Fiscal Year: 2008
    ..Finally, in the experiments described here, mechanical force will be used as a new controllable variable to characterize the process of mechanochemical transduction in this motor enzyme. ..
  16. Antigenic structure of adeno-associated virus capsids and antibody escape mutants
    Colin Parrish; Fiscal Year: 2008
    ..In this study we seek to define the antigenic structures if the viruses so that the adverse effects of antibodies on gene therapy trials can be better understood, and perhaps avoided in the future. [unreadable] [unreadable] [unreadable]..
  17. Densely Multiplexed Fluorescence Imaging by Fourier Transform Fluorometry
    Leilei Peng; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  18. Functionalized Star Polymer Nanoparticles for Controlled Drug Delivery
    Fei Wang; Fiscal Year: 2008
    ..The polymer/drug medication will minimize early degradation of the encapsulated drug and decrease harmful side-effects from premature drug release. [unreadable] [unreadable] [unreadable] [unreadable]..
  19. The biology of gastrin-ferric ion complexes
    GRAHAM BALDWIN; Fiscal Year: 2009
    ..Recognition that metal ions are essential for the biological activity of NAGs may permit the development of novel therapies for colon cancer. ..
  20. Comprehensive Magnetic Resonance in PAD
    Christopher Kramer; Fiscal Year: 2008
    ..Improving the health outcomes and quality of life in patients with PAD is a major goal of this proposal. ..
  21. Trekking with the Ribognome: Single Molecule Microscopy of Intracellular miRNPs
    Nils G Walter; Fiscal Year: 2010
    ..concept? What are the lower limits for DNA repeat sequence and protein polymerization detection and can these limits be further pushed? Can multiple different biopolymers be detected in parallel and how does this affect detection limits? ..
    Colin R Parrish; Fiscal Year: 2011
    ..We would seek to complement endosomal escape mutants in trans by adding adenovirus capsids, competent wild type parvovirus, or transferrin-PEI conjugates to the cells along with the parvoviruses. ..
  23. In Vivo Characterization of Bacteria-mediated Extracellular Reduction of Chromium
    H Peter Lu; Fiscal Year: 2011
  24. Sugar signaling networks detected by high content fluxomics
    Wolf B Frommer; Fiscal Year: 2010
    ..The networks and databases developed in this project promise to provide novel screening methods for novel drugs as well as help identifying new drug targets. ..
  25. Spectral-Encoding for Video-Rate Hemodynamic Tomography
    Brian Pogue; Fiscal Year: 2007
    ..abstract_text> ..
    Christopher Kramer; Fiscal Year: 2002
    ..Effects of beta-blockade on regional cell morphometry, collagen content, and blood flow will be assessed using isolated myocytes, quantitative histopathology, and fluorescent microspheres, respectively. ..
  27. In Vivo fluorescence correlation spectroscopy
    Yan Chen; Fiscal Year: 2003
    ..In addition, GFP tagged retinoid receptors will be used to perform an in vivo study to resolve their molecular oligomerization state in the presence and absence of ligands. ..
    Fei Wang; Fiscal Year: 2004
    ..abstract_text> ..
    Ira Ropson; Fiscal Year: 2003
    ..Single-site mutant proteins will be generated and studied for their effects on the transition state in protein folding. ..
    YUAN LEE; Fiscal Year: 2005
    ..These studies are significant from the evolutionary standpoint, since the occurrence of alpha-Gal in glycoproteins is rare in birds, and the responsible transferase seems to be different from that of mammals. ..
    Fei Wang; Fiscal Year: 2006
    ..New drug delivery systems have had an impact on nearly every branch of medicine. Controlled drug delivery is a $20 billion industry and rising rapidly...
    Douglas Borchman; Fiscal Year: 2003
    ..An understanding of these alterations will facilitate the development of therapies to prevent or perhaps even reverse these changes. ..
  33. Cytokine signal transduction in immune system
    Hodaka Fujii; Fiscal Year: 2007
    ..Detailed information gained from the proposed experiments will be valuable for elucidation of molecular mechanisms of oncogenesis and autoimmune diseases caused by dysregulation of cytokine signaling. ..
    Colin Parrish; Fiscal Year: 2007
    ..abstract_text> ..
  35. Mercury exposure and the innate immune response in autoimmune heart disease
    Jennifer Nyland; Fiscal Year: 2008
    ..Specific Aim 3. To test the hypothesis that: Human exposure to Hg induces changes in the immune system that are measurable and indicative of elevated risk of autoimmune dysfunction. ..
  36. Magnetic Resonance Imaging of Alzheimer's Amyloid Plaque
    JOSEPH PODUSLO; Fiscal Year: 2007
    ..unreadable] [unreadable]..
    JOSEPH PODUSLO; Fiscal Year: 2003
    ..This grant proposal, therefore, will further test whether this hypothesis-driven therapy is beneficial in the treatment of FALS transgenic mice, which will have direct implications for the treatment of both familial and sporadic ALS. ..
  38. New Technology for Selective Delivery of PNAs in Cancer Cells In Vitro and In Viv
    OLEG ANDREEV; Fiscal Year: 2008
    ..The pHLIP nanosyringe could be a very effective tool for molecular analysis of cancer cells and diagnosis and treatment of cancer. [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
    Robert Phillips; Fiscal Year: 2004
    ..Thus the results of this research are likely to make a significant contribution to the goal of improving health care for Americans in the future. ..
  40. Fluorescent Zinc Sensors for Cellular Imaging
    CHRISTOPH FAHRNI; Fiscal Year: 2008
    ..The lack of a non-invasive zinc specific fluorescent sensor is currently a limiting factor in the study of intracellular zinc regulation and trafficking. ..