hydrogen peroxide


Summary: A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.

Top Publications

  1. pmc How mitochondria produce reactive oxygen species
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB20XY, UK
    Biochem J 417:1-13. 2009
  2. pmc Genomic expression programs in the response of yeast cells to environmental changes
    A P Gasch
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5428, USA
    Mol Biol Cell 11:4241-57. 2000
  3. ncbi Oxidative stress, antioxidants and stress tolerance
    Ron Mittler
    Dept of Botany, Plant Sciences Institute, 353 Bessey Hall, Iowa State University, Ames, IA 50011, USA
    Trends Plant Sci 7:405-10. 2002
  4. ncbi A common mechanism of cellular death induced by bactericidal antibiotics
    Michael A Kohanski
    Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 130:797-810. 2007
  5. pmc A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish
    Philipp Niethammer
    Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 459:996-9. 2009
  6. ncbi Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
    Vsevolod V Belousov
    Laboratory of Genes for Regeneration, Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya Street 16 10, Moscow 117997, Russia
    Nat Methods 3:281-6. 2006
  7. pmc Cellular defenses against superoxide and hydrogen peroxide
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Annu Rev Biochem 77:755-76. 2008
  8. pmc Global transcriptional responses of fission yeast to environmental stress
    Dongrong Chen
    The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
    Mol Biol Cell 14:214-29. 2003
  9. pmc Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1
    M Saitoh
    Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research, 1 37 1 Kami Ikebukuro, Toshima ku, Tokyo 170, Japan
    EMBO J 17:2596-606. 1998
  10. ncbi Myeloperoxidase: friend and foe
    Seymour J Klebanoff
    Department of Medicine, University of Washington School of Medicine, Seattle, WA 98195 7185, USA
    J Leukoc Biol 77:598-625. 2005

Detail Information

Publications350 found, 100 shown here

  1. pmc How mitochondria produce reactive oxygen species
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB20XY, UK
    Biochem J 417:1-13. 2009
  2. pmc Genomic expression programs in the response of yeast cells to environmental changes
    A P Gasch
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5428, USA
    Mol Biol Cell 11:4241-57. 2000
    ..in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing ..
  3. ncbi Oxidative stress, antioxidants and stress tolerance
    Ron Mittler
    Dept of Botany, Plant Sciences Institute, 353 Bessey Hall, Iowa State University, Ames, IA 50011, USA
    Trends Plant Sci 7:405-10. 2002
    ..These raise several intriguing questions about the relationships between ROI signaling, ROI stress and the production and scavenging of ROIs in the different cellular compartments...
  4. ncbi A common mechanism of cellular death induced by bactericidal antibiotics
    Michael A Kohanski
    Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 130:797-810. 2007
    ..g., reca...
  5. pmc A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish
    Philipp Niethammer
    Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 459:996-9. 2009
    ..Owing to their fast diffusion and versatile biological activities, reactive oxygen species, including hydrogen peroxide (H(2)O(2)), are interesting candidates for wound-to-leukocyte signalling...
  6. ncbi Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
    Vsevolod V Belousov
    Laboratory of Genes for Regeneration, Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya Street 16 10, Moscow 117997, Russia
    Nat Methods 3:281-6. 2006
    We developed a genetically encoded, highly specific fluorescent probe for detecting hydrogen peroxide (H(2)O(2)) inside living cells...
  7. pmc Cellular defenses against superoxide and hydrogen peroxide
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Annu Rev Biochem 77:755-76. 2008
    ..They also developed mechanisms to defend themselves against superoxide (O(2)()) and hydrogen peroxide, partially reduced oxygen species that are generated as inadvertent by-products of aerobic metabolism...
  8. pmc Global transcriptional responses of fission yeast to environmental stress
    Dongrong Chen
    The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
    Mol Biol Cell 14:214-29. 2003
    ..profiles of all known and predicted genes in response to five stress conditions: oxidative stress caused by hydrogen peroxide, heavy metal stress caused by cadmium, heat shock caused by temperature increase to 39 degrees C, osmotic ..
  9. pmc Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1
    M Saitoh
    Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research, 1 37 1 Kami Ikebukuro, Toshima ku, Tokyo 170, Japan
    EMBO J 17:2596-606. 1998
    ..Treatment of cells with N-acetyl-L-cysteine also inhibited serum withdrawal-, TNF-alpha- and hydrogen peroxide-induced activation of ASK1 as well as apoptosis...
  10. ncbi Myeloperoxidase: friend and foe
    Seymour J Klebanoff
    Department of Medicine, University of Washington School of Medicine, Seattle, WA 98195 7185, USA
    J Leukoc Biol 77:598-625. 2005
    ..is one consisting of myeloperoxidase (MPO), released into the phagosome during the degranulation process, hydrogen peroxide (H2O2), formed by the respiratory burst and a halide, particularly chloride...
  11. ncbi Salidroside protects human erythrocytes against hydrogen peroxide-induced apoptosis
    Erin Wei Qian
    Programme of Biochemistry, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, People s Republic of China
    J Nat Prod 75:531-7. 2012
    ..Compound 1 is a protective agent in human erythrocytes against oxidative stress and may be a good adaptogen to enhance the body's resistance to stress and fatigue...
  12. ncbi Cell signaling. H2O2, a necessary evil for cell signaling
    Sue Goo Rhee
    Institute of Molecular Life Science and Technology, Ewha Women s University, Seoul 120 750, South Korea
    Science 312:1882-3. 2006
  13. ncbi NF-kappaB activation by reactive oxygen species: fifteen years later
    Geoffrey Gloire
    Center for Biomedical Integrated Genoproteomics CBIG, Virology and Immunology Unit, University of Liege, 4000 Liege, Belgium
    Biochem Pharmacol 72:1493-505. 2006
    ..Finally, we critically review the recent data highlighting the role of ROS in NF-kappaB activation by proinflammatory cytokines (TNF-alpha and IL-1beta) and lipopolysaccharide (LPS), two major components of innate immunity...
  14. ncbi Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader
    H Wang
    Neuroscience Laboratory, USDA ARS, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
    Free Radic Biol Med 27:612-6. 1999
    ..This method is reliable and efficient for evaluating the potency of pro-oxidants and can be used to evaluate the efficacy of antioxidants against OS in cells...
  15. ncbi Topology of superoxide production from different sites in the mitochondrial electron transport chain
    Julie St-Pierre
    MRC Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, England
    J Biol Chem 277:44784-90. 2002
    ..By measuring hydrogen peroxide production in the absence and presence of exogenous superoxide dismutase, we established the topology of ..
  16. ncbi Mediation of poly(ADP-ribose) polymerase-1-dependent cell death by apoptosis-inducing factor
    Seong Woon Yu
    Department of Neurology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Science 297:259-63. 2002
    ..These data support a model in which PARP-1 activation signals AIF release from mitochondria, resulting in a caspase-independent pathway of programmed cell death...
  17. ncbi Hydrogen peroxide regulation of endothelial function: origins, mechanisms, and consequences
    Hua Cai
    Section of Cardiology, Department of Medicine, The Division of Biological Sciences and Pritzker School of Medicine, The University of Chicago, 5841 S Maryland Ave, MC6088, Chicago, IL 60637, USA
    Cardiovasc Res 68:26-36. 2005
    ..O2(*-) rapidly degrades by reacting with nitric oxide (NO*), the O2*- signal preserved by dismutation into hydrogen peroxide (H2O2) exerts prolonged signaling effects...
  18. ncbi Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes
    Gerd P Bienert
    Department of Agricultural Sciences, Faculty of Life Science, Copenhagen University, DK 1871 Frederiksberg C, Denmark
    J Biol Chem 282:1183-92. 2007
    ..g. to cross membranes. Here we present evidence that aquaporins can channel hydrogen peroxide (H2O2)...
  19. pmc Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues
    Qi Chen
    Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:13604-9. 2005
    ..These findings give plausibility to i.v. ascorbic acid in cancer treatment, and have unexpected implications for treatment of infections where H(2)O(2) may be beneficial...
  20. ncbi Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing
    Robert D Guzy
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    Cell Metab 1:401-8. 2005
    ..These results demonstrate that mitochondria function as O(2) sensors and signal hypoxic HIF-1 alpha and HIF-2 alpha stabilization by releasing ROS to the cytosol...
  21. pmc Distinct roles of Nox1 and Nox4 in basal and angiotensin II-stimulated superoxide and hydrogen peroxide production
    Sergey I Dikalov
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, 1639 Pierce Drive, Atlanta, GA 30322, USA
    Free Radic Biol Med 45:1340-51. 2008
    NADPH oxidases are major sources of superoxide (O2*-) and hydrogen peroxide (H2O2) in vascular cells...
  22. pmc Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response
    Miguel Angel Torres
    Department of Biology and Curriculum in Genetics and Molecular Biology, CB 3280, 108 Coker Hall, University of North Carolina, Chapel Hill, NC 27599 3280, USA
    Proc Natl Acad Sci U S A 99:517-22. 2002
    ..parasitica. Paradoxically, although atrbohD mutation eliminated the majority of total ROI production, atrbohF mutation exhibited the strongest effect on cell death...
  23. pmc Exposure to hydrogen peroxide induces oxidation and activation of AMP-activated protein kinase
    Jaroslaw W Zmijewski
    Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 0012, USA
    J Biol Chem 285:33154-64. 2010
    ..The present findings also imply that AMPK activation, in addition to being a response to alterations in intracellular metabolic pathways, is directly influenced by cellular redox status...
  24. pmc In vaginal fluid, bacteria associated with bacterial vaginosis can be suppressed with lactic acid but not hydrogen peroxide
    Deirdre E O'Hanlon
    Mucosal Protection Laboratory, Thomas C Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    BMC Infect Dis 11:200. 2011
    b>Hydrogen peroxide (H2O2) produced by vaginal lactobacilli is generally believed to protect against bacteria associated with bacterial vaginosis (BV), and strains of lactobacilli that can produce H2O2 are being developed as vaginal ..
  25. ncbi Myeloperoxidase associated with neutrophil extracellular traps is active and mediates bacterial killing in the presence of hydrogen peroxide
    Heather Parker
    Department of Pathology, University of Otago Christchurch, 2 Riccarton Ave, Christchurch, New Zealand
    J Leukoc Biol 91:369-76. 2012
    ..Our results show that the enzymatic activity of MPO on NETs could contribute to antimicrobial activity or tissue injury when NETs are released from neutrophils at sites of infection or inflammation...
  26. pmc Lyn is a redox sensor that mediates leukocyte wound attraction in vivo
    Sa Kan Yoo
    Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Nature 480:109-12. 2011
    ..Wounds and tumours--a type of 'unhealed wound'--generate hydrogen peroxide (H(2)O(2)) through an NADPH oxidase (NOX)...
  27. ncbi ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis
    Jo Bright
    Centre for Research in Plant Science, Genomics Research Institute, University of the West of England, UWE, Bristol, Frenchay Campus, Coldharbour Lane, UK
    Plant J 45:113-22. 2006
    Nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) are key signalling molecules produced in response to various stimuli and involved in a diverse range of plant signal transduction processes...
  28. ncbi Inactivation of a peroxiredoxin by hydrogen peroxide is critical for thioredoxin-mediated repair of oxidized proteins and cell survival
    Alison M Day
    Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, Tyne and Wear, UK
    Mol Cell 45:398-408. 2012
    ..Here we demonstrate that, in cells treated with hydrogen peroxide, the Prx Tpx1 is a major substrate for thioredoxin in the fission yeast Schizosaccharomyces pombe and, as ..
  29. pmc Duox, Flotillin-2, and Src42A are required to activate or delimit the spread of the transcriptional response to epidermal wounds in Drosophila
    Michelle T Juarez
    Section of Cell and Developmental Biology, Division of Biology, University of California San Diego, La Jolla, California, United States of America
    PLoS Genet 7:e1002424. 2011
    ..One gene required to activate epidermal wound reporters encodes Dual oxidase, an enzyme that produces hydrogen peroxide. We also find that four biochemical treatments (a serine protease, a Src kinase inhibitor, methyl-ß-..
  30. ncbi Hydrogen peroxide sensing and signaling
    Elizabeth A Veal
    Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne, Tyne and Wear, UK
    Mol Cell 26:1-14. 2007
    ..contain antioxidant enzymes that limit oxidative stress by detoxifying reactive oxygen species, including hydrogen peroxide. However, in eukaryotes, hydrogen peroxide also has important roles as a signaling molecule in the ..
  31. ncbi A caspase-related protease regulates apoptosis in yeast
    Frank Madeo
    Institute for Physiological Chemistry, University of Tubingen, Hoppe Seyler Strasse 4, 72076 Tubingen, Germany
    Mol Cell 9:911-7. 2002
    ..b>Hydrogen peroxide treatment induces apoptosis together with a caspase-like enzymatic activity in yeast...
  32. ncbi Complex III releases superoxide to both sides of the inner mitochondrial membrane
    Florian L Muller
    Department of Cellular Biology, University of Texas Health Science Center, San Antonio, TX 78284 7762, USA
    J Biol Chem 279:49064-73. 2004
    ..In the present work, using measurements of hydrogen peroxide (Amplex red) as well as superoxide (modified Cypridina luciferin analog and aconitase), we demonstrate that ..
  33. ncbi Nitric oxide, stomatal closure, and abiotic stress
    Steven Neill
    Centre for Research in Plant Science, Faculty of Health and Life Sciences, University of the West of England, Bristol BS16 1QY, UK
    J Exp Bot 59:165-76. 2008
    ..These are two processes that both involve NO as a key signalling intermediate...
  34. pmc Catabolite control protein A controls hydrogen peroxide production and cell death in Streptococcus sanguinis
    Lanyan Zheng
    Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Bacteriol 193:516-26. 2011
    Streptococcus sanguinis is a commensal oral bacterium producing hydrogen peroxide (H₂O₂) that is dependent on pyruvate oxidase (Spx) activity...
  35. pmc Oxidative stress, caloric restriction, and aging
    R S Sohal
    Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275, USA
    Science 273:59-63. 1996
    ..inversely correlate with the rates of mitochondrial generation of the superoxide anion radical (O2) and hydrogen peroxide. (iii) Restriction of caloric intake lowers steady-state levels of oxidative stress and damage, retards age-..
  36. ncbi Reversible inactivation of the tumor suppressor PTEN by H2O2
    Seung Rock Lee
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 8015, USA
    J Biol Chem 277:20336-42. 2002
  37. pmc Oxidation of tetrahydrobiopterin leads to uncoupling of endothelial cell nitric oxide synthase in hypertension
    Ulf Landmesser
    Division of Cardiology, Emory University School of Medicine and Atlanta Veterans Administration Hospital, 1639 Pierce Drive, Atlanta, GA 30322, USA
    J Clin Invest 111:1201-9. 2003
    ..is only minimally altered in vessels of mice with DOCA-salt hypertension but seems to be mediated by hydrogen peroxide released from uncoupled eNOS, since it is inhibited by catalase...
  38. ncbi Hydrogen peroxide: a metabolic by-product or a common mediator of ageing signals?
    Marco Giorgio
    Marco Giorgio, Enrica Migliaccio and Pier Giuseppe Pelicci are at the Department of Experimental Oncology, IEO European Institute of Oncology, and IFOM FIRC Institute of Molecular Oncology, 20139 Milan, Italy
    Nat Rev Mol Cell Biol 8:722-8. 2007
    The reactive oxygen species that are generated by mitochondrial respiration, including hydrogen peroxide (H2O2), are potent inducers of oxidative damage and mediators of ageing...
  39. ncbi ROS, mitochondria and the regulation of autophagy
    Ruth Scherz-Shouval
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel
    Trends Cell Biol 17:422-7. 2007
    ..We then discuss the relationship between mitochondria and autophagosomes and propose that mitochondria have an essential role in autophagosome biogenesis...
  40. ncbi Reactive oxygen species: a breath of life or death?
    John P Fruehauf
    Department of Medicine, Chao Family Comprehensive Cancer Center, University of California, Irvine, Orange, California 92668, USA
    Clin Cancer Res 13:789-94. 2007
    ..This review will cover recent work in ROS-mediated signaling in cancer cells and its potential as a target for developmental therapeutics...
  41. pmc DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide
    M Zheng
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 183:4562-70. 2001
    The genome-wide transcription profile of Escherichia coli cells treated with hydrogen peroxide was examined with a DNA microarray composed of 4,169 E. coli open reading frames...
  42. ncbi Hydrogen peroxide and nitric oxide as signalling molecules in plants
    Steven J Neill
    Centre for Research in Plant Science, Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK
    J Exp Bot 53:1237-47. 2002
    It is now clear that hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) function as signalling molecules in plants. A wide range of abiotic and biotic stresses results in H(2)O(2) generation, from a variety of sources...
  43. pmc The E-loop is involved in hydrogen peroxide formation by the NADPH oxidase Nox4
    Ina Takac
    Institut für Kardiovaskuläre Physiologie, Goethe Universitat, 60596 Frankfurt am Main, Germany
    J Biol Chem 286:13304-13. 2011
    In contrast to the NADPH oxidases Nox1 and Nox2, which generate superoxide (O(2)(·-)), Nox4 produces hydrogen peroxide (H(2)O(2))...
  44. ncbi Rapamycin sensitive mTOR activation mediates nerve growth factor (NGF) induced cell migration and pro-survival effects against hydrogen peroxide in retinal pigment epithelial cells
    Guo Fan Cao
    The Affiliated Eye Hospital of Nanjing Medical University, Nanjing 210029, China
    Biochem Biophys Res Commun 414:499-505. 2011
    ..human retinal pigment epithelial (RPE) cells, we demonstrate here that NGF protects RPE cells against hydrogen peroxide (H(2)O(2))-induced cell apoptosis...
  45. pmc Cancer cells metabolically "fertilize" the tumor microenvironment with hydrogen peroxide, driving the Warburg effect: implications for PET imaging of human tumors
    Ubaldo E Martinez-Outschoorn
    The Jefferson Stem Cell Biology and Regenerative Medicine Center, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA
    Cell Cycle 10:2504-20. 2011
    ..Interestingly, using this approach, we directly show that cancer cells initially secrete hydrogen peroxide that then triggers oxidative stress in neighboring fibroblasts...
  46. ncbi Mitochondrial free radical generation, oxidative stress, and aging
    E Cadenas
    Department of Molecular Pharmacology and Toxicology, School of Pharmacy University of Southern California, Los Angeles 90033, USA
    Free Radic Biol Med 29:222-30. 2000
    ..chain with molecular oxygen directly generate the superoxide anion radical (O2*-), which dismutates to form hydrogen peroxide (H2O2), which can further react to form the hydroxyl radical (HO*)...
  47. ncbi Ginsenoside Rg1 protects against hydrogen peroxide-induced cell death in PC12 cells via inhibiting NF-κB activation
    Qian Liu
    Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, PR China
    Neurochem Int 58:119-25. 2011
    ..However, its molecular mechanism against oxidative stress induced by exogenous hydrogen peroxide (H(2)O(2)) remained unclear...
  48. ncbi Hydrogen peroxide: a signaling messenger
    James R Stone
    Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Antioxid Redox Signal 8:243-70. 2006
    b>Hydrogen peroxide (H2O2) is a well-documented component of living cells. It plays important roles in host defense and oxidative biosynthetic reactions...
  49. pmc Towards a unifying, systems biology understanding of large-scale cellular death and destruction caused by poorly liganded iron: Parkinson's, Huntington's, Alzheimer's, prions, bactericides, chemical toxicology and others as examples
    Douglas B Kell
    School of Chemistry and The Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester M1 7DN, UK
    Arch Toxicol 84:825-89. 2010
    ..A variety of systems biology approaches, that I summarise, can predict both the mechanisms involved in these cell death pathways and the optimal sites of action for nutritional or pharmacological interventions...
  50. ncbi Oxidative stress and nuclear factor-kappaB activation: a reassessment of the evidence in the light of recent discoveries
    A Bowie
    Department of Biochemistry, Trinity College, Dublin, Ireland
    Biochem Pharmacol 59:13-23. 2000
    ..In particular, the activation of NFkappaB by hydrogen peroxide is cell-specific and distinct from physiological activators such as IL-1 and TNF, while inhibition by ..
  51. ncbi Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling
    Zachary A Wood
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97333, USA
    Science 300:650-3. 2003
    Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction...
  52. pmc Generating disulfides enzymatically: reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p
    Einav Gross
    Department of Structural Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 103:299-304. 2006
    ..Under aerobic conditions, reduction of molecular oxygen by Ero1p yielded stoichiometric hydrogen peroxide. Remarkably, we found that reduced Ero1p can transfer electrons to a variety of small and macromolecular ..
  53. pmc Could heat shock transcription factors function as hydrogen peroxide sensors in plants?
    Gad Miller
    Department of Biochemistry and Molecular Biology, University of Nevada, Mail Stop 200, Reno, NV 89557, USA
    Ann Bot 98:279-88. 2006
  54. ncbi Activated granulocytes and granulocyte-derived hydrogen peroxide are the underlying mechanism of suppression of t-cell function in advanced cancer patients
    J Schmielau
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Cancer Res 61:4756-60. 2001
    ..This action is abrogated by the addition of the hydrogen peroxide (H(2)O(2)) scavenger, catalase, implicating H(2)O(2) as the effector molecule...
  55. pmc Superoxide dismutase in redox biology: the roles of superoxide and hydrogen peroxide
    Garry R Buettner
    Free Radical and Radiation Biology, The University of Iowa, Iowa City, 52242 1181, USA
    Anticancer Agents Med Chem 11:341-6. 2011
    ..These observations indicated that SOD, superoxide, and hydrogen peroxide affected the basic biology of cells and tissues, not just via damaging oxidation reactions...
  56. ncbi Rutin inhibits hydrogen peroxide-induced apoptosis through regulating reactive oxygen species mediated mitochondrial dysfunction pathway in human umbilical vein endothelial cells
    Guohua Gong
    Institute for Nanobiomedical Technology and Membrane Biology, State Key Lab of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, Sichuan, PR China
    Eur J Pharmacol 628:27-35. 2010
    ..protective effects of rutin against apoptosis of human umbilical vein endothelial cells (HUVECs) induced by hydrogen peroxide (H(2)O(2)) and the associated signaling pathways...
  57. ncbi Hydrogen peroxide production by Lactobacillus johnsonii NCC 533 and its role in anti-Salmonella activity
    Raymond David Pridmore
    Department of Nutrition and Health, Nestle Research Center, Vers chez les Blancs, Lausanne, Switzerland
    FEMS Microbiol Lett 283:210-5. 2008
    ..identified three potential gene products that may catalyze the synthesis of the known antimicrobial factor hydrogen peroxide, H(2)O(2). In this work, we confirmed the ability of NCC 533 as well as eight different L...
  58. ncbi Association of oxidative stress with postmenopausal osteoporosis and the effects of hydrogen peroxide on osteoclast formation in human bone marrow cell cultures
    Ki Hyun Baek
    Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea
    Calcif Tissue Int 87:226-35. 2010
    ..In addition, RANKL and M-CSF stimulation induced by oxidative stress may participate in osteoclastogenesis in human bone...
  59. pmc Human umbilical cord blood-derived mesenchymal stem cells undergo cellular senescence in response to oxidative stress
    Eun Ko
    Department of Biological Sciences, Seoul National University, Seoul, Korea
    Stem Cells Dev 21:1877-86. 2012
    ..Therefore, the proliferation and fate of hUCB-MSCs can be controlled by exposure to oxidative stresses...
  60. pmc A hydrogen peroxide-inactivated virus vaccine elicits humoral and cellular immunity and protects against lethal West Nile virus infection in aged mice
    Amelia K Pinto
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    J Virol 87:1926-36. 2013
    ..Here, we describe the generation and preclinical efficacy of a hydrogen peroxide (H(2)O(2))-inactivated WNV Kunjin strain (WNV-KUNV) vaccine as a candidate for further development...
  61. ncbi A Mitogen-activated protein kinase kinase kinase mediates reactive oxygen species homeostasis in Arabidopsis
    Hirofumi Nakagami
    Department of Plant Molecular Biology, Max F Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, A 1030 Vienna, Austria
    J Biol Chem 281:38697-704. 2006
    ..Overall, our data defines MPK4 as downstream target of MEKK1 and show that MEKK1 functions in integrating ROS homeostasis with plant development and hormone signaling...
  62. pmc Molecular evolution of hydrogen peroxide degrading enzymes
    Marcel Zamocky
    Division of Biochemistry, Department of Chemistry, Vienna Institute of Biotechnology at BOKU University of Natural Resources and Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    Arch Biochem Biophys 525:131-44. 2012
    For efficient removal of intra- and/or extracellular hydrogen peroxide by dismutation to harmless dioxygen and water (2H(2)O(2) → O(2) + 2H(2)O), nature designed three metalloenzyme families that differ in oligomeric organization, ..
  63. pmc Oxidative protein folding by an endoplasmic reticulum-localized peroxiredoxin
    Ester Zito
    Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 40:787-97. 2010
    ..These observations implicate ER-localized PRDX4 in a previously unanticipated, parallel, ERO1-independent pathway that couples hydroperoxide production to oxidative protein folding in mammalian cells...
  64. ncbi In vivo mapping of hydrogen peroxide and oxidized glutathione reveals chemical and regional specificity of redox homeostasis
    Simone C Albrecht
    Division of Redox Regulation, DKFZ ZMBH Alliance, German Cancer Research Center DKFZ, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Cell Metab 14:819-29. 2011
    The glutathione redox couple (GSH/GSSG) and hydrogen peroxide (H(2)O(2)) are central to redox homeostasis and redox signaling, yet their distribution within an organism is difficult to measure...
  65. pmc Hydrogen peroxide: a Jekyll and Hyde signalling molecule
    D R Gough
    Tumour Biology Laboratory, Biochemistry Department, Bioscience Research Institute, University College Cork, Cork, Ireland
    Cell Death Dis 2:e213. 2011
    ..Endogenous ROS such as hydrogen peroxide (H₂O₂) have long been recognised as destructive molecules...
  66. pmc Peroxiredoxin functions as a peroxidase and a regulator and sensor of local peroxides
    Sue Goo Rhee
    Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul 120 750, Korea
    J Biol Chem 287:4403-10. 2012
    ..The fact that the oxidation state of the active site cysteine of Prx can be transferred to other proteins that are less intrinsically susceptible to H(2)O(2) also allows Prx to function as an H(2)O(2) sensor...
  67. pmc Oxidative stress activates a positive feedback between the gamma- and beta-secretase cleavages of the beta-amyloid precursor protein
    Elena Tamagno
    Department of Experimental Medicine and Oncology, University of Torino, Torino, Italy
    J Neurochem 104:683-95. 2008
    ..Our findings suggest a mechanism by which OS induces BACE1 transcription, thereby promoting production of pathological levels of amyloid beta in AD...
  68. pmc Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling
    Fan Lin
    College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People s Republic of China
    J Exp Bot 60:3221-38. 2009
    ..The results suggest that there is a positive feedback loop involving NADPH oxidase, H(2)O(2), and ZmMPK5 in ABA signalling...
  69. ncbi JunD reduces tumor angiogenesis by protecting cells from oxidative stress
    Damien Gerald
    Unit of Gene Expression and Diseases, CNRS URA 1644, Pasteur Institute, 25 rue du Docteur Roux, 75724 Paris, Cedex 15, France
    Cell 118:781-94. 2004
    ..Furthermore, we provide new insights into the regulation of PHD activity, allowing immediate reactive adaptation to changes in O2 or iron levels in the cell...
  70. ncbi Photo-irradiated titanium dioxide catalyzes site specific DNA damage via generation of hydrogen peroxide
    Kazutaka Hirakawa
    Department of Radiation Chemistry, Life Science Research Center, Mie University, Edobashi 2 174, Tsu Mie 514 8507, Japan
    Free Radic Res 38:439-47. 2004
    ..SOD and bathocuproine, a chelator of Cu(I), inhibited the DNA damage, suggesting the involvement of hydrogen peroxide, superoxide and Cu(I)...
  71. pmc Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis
    Sholpan Davletova
    Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Nevada 89557, USA
    Plant Cell 17:268-81. 2005
    ..Our study points to a key role for the cytosol in protecting the chloroplast during light stress and provides evidence for cross-compartment protection of thylakoid and stromal/mitochondrial APXs by cytosolic APX1...
  72. ncbi Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators
    Colette T Dooley
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093 0647, USA
    J Biol Chem 279:22284-93. 2004
    ..g. epidermal growth factor and lysophosphatidic acid...
  73. ncbi Identification of the NF-E2-related factor-2-dependent genes conferring protection against oxidative stress in primary cortical astrocytes using oligonucleotide microarray analysis
    Jong Min Lee
    School of Pharmacy, Molecular and Environmental Toxicology Center, University of Wisconsin, Madison, Wisconsin 53705, USA
    J Biol Chem 278:12029-38. 2003
  74. ncbi OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis
    Maike C Rentel
    Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, UK
    Nature 427:858-61. 2004
    ..Thus, OXI1 is an essential part of the signal transduction pathway linking oxidative burst signals to diverse downstream responses...
  75. pmc An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses
    Yuchen Miao
    Henan Key Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng 475001, China
    Plant Cell 18:2749-66. 2006
  76. pmc Kallikrein-modified mesenchymal stem cell implantation provides enhanced protection against acute ischemic kidney injury by inhibiting apoptosis and inflammation
    Makoto Hagiwara
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Hum Gene Ther 19:807-19. 2008
    ..These results indicate that implantation of kallikrein-modified MSCs in the kidney provides advanced benefits in protection against ischemia-induced kidney injury by suppression of apoptosis and inflammation...
  77. pmc Activation of dual oxidases Duox1 and Duox2: differential regulation mediated by camp-dependent protein kinase and protein kinase C-dependent phosphorylation
    Sabrina Rigutto
    Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire, Université Libre Bruxelles, Brussels, Belgium
    J Biol Chem 284:6725-34. 2009
    ..Our data provide, for the first time, detailed insights into the mechanisms controlling the activation of Duox1-2 proteins and reveal additional phosphorylation-mediated regulation...
  78. ncbi Hydrogen peroxide signalling
    Steven Neill
    Centre for Research in Plant Science, Faculty of Applied Sciences, University of the West of England UWE, Frenchay, Bristol, BS161QY, UK
    Curr Opin Plant Biol 5:388-95. 2002
    Recent biochemical and genetic studies confirm that hydrogen peroxide is a signalling molecule in plants that mediates responses to abiotic and biotic stresses...
  79. ncbi Redox signaling: hydrogen peroxide as intracellular messenger
    S G Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Exp Mol Med 31:53-9. 1999
    ..The goal of this report is to review our current knowledge of ROS-regulated signal transduction and propose future directions...
  80. ncbi The apoplastic oxidative burst in response to biotic stress in plants: a three-component system
    G Paul Bolwell
    Division of Biochemistry, School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK
    J Exp Bot 53:1367-76. 2002
    ..Thus it is possible that Arabidopsis is another species with the potential to mount an apoplastic oxidative burst and these transformed plant lines may be useful to identify the peroxidase that is responsible...
  81. ncbi Hydrogen peroxide as second messenger in lymphocyte activation
    Michael Reth
    Max Planck Institute für Immunobiologie, Universitat Freiburg, Biologie III, Abteilung Molekulare Immunologie, Stuebeweg 51, Freiburg 79108, Germany
    Nat Immunol 3:1129-34. 2002
    ..Thus, by controlling the activity of the negative regulatory phosphatases inside the cell, H2O2 can set and influence critical thresholds for lymphocyte activation...
  82. pmc Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: another inconvenient truth
    Jacek Zielonka
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 48:983-1001. 2010
  83. ncbi Regeneration of peroxiredoxins by p53-regulated sestrins, homologs of bacterial AhpD
    Andrei V Budanov
    Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Science 304:596-600. 2004
    Acting as a signal, hydrogen peroxide circumvents antioxidant defense by overoxidizing peroxiredoxins (Prxs), the enzymes that metabolize peroxides...
  84. pmc Increased oxidative stress in obesity and its impact on metabolic syndrome
    Shigetada Furukawa
    Department of Medicine and Pathophysiology, Graduate School of Medicine, Department of Organismal Biosystems, Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Clin Invest 114:1752-61. 2004
  85. ncbi Trehalose accumulation during cellular stress protects cells and cellular proteins from damage by oxygen radicals
    N Benaroudj
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:24261-7. 2001
    ..Therefore, trehalose accumulation in stressed cells plays a major role in protecting cellular constituents from oxidative damage...
  86. ncbi Angiotensin II stimulation of NAD(P)H oxidase activity: upstream mediators
    Puvi N Seshiah
    Division of Cardiology, Emory University, Atlanta, GA 30322, USA
    Circ Res 91:406-13. 2002
  87. ncbi Reactive oxygen species as a signal in glucose-stimulated insulin secretion
    Jingbo Pi
    Endocrine Biology Program, The Hamner Institutes for Health Sciences, 6 Davis Dr, Research Triangle Park, NC 27709, USA
    Diabetes 56:1783-91. 2007
    ..Taken together, these findings suggest that H2O2 derived from glucose metabolism is one of the metabolic signals for insulin secretion, whereas oxidative stress may disturb its signaling function...
  88. ncbi Mitochondrial DNA mutations, oxidative stress, and apoptosis in mammalian aging
    G C Kujoth
    Departments of Genetics and Medical Genetics, University of Wisconsin, Madison, WI 53706, USA
    Science 309:481-4. 2005
    ..The levels of apoptotic markers were also found to increase during aging in normal mice. Thus, accumulation of mtDNA mutations that promote apoptosis may be a central mechanism driving mammalian aging...
  89. ncbi Blocking late sodium current reduces hydrogen peroxide-induced arrhythmogenic activity and contractile dysfunction
    Yejia Song
    Division of Cardiovascular Medicine, University of Florida, 1600 SW Archer Rd, M 411, Gainesville, FL 32610 0277, USA
    J Pharmacol Exp Ther 318:214-22. 2006
  90. ncbi Mitochondrial alpha-ketoglutarate dehydrogenase complex generates reactive oxygen species
    Anatoly A Starkov
    Department of Neurology and Neuroscience, Weill Medical College, Cornell University, New York, New York 10021, USA
    J Neurosci 24:7779-88. 2004
    ..These mitochondria also produced significantly less H(2)O(2) than mitochondria isolated from their littermate wild-type mice. The data strongly indicate that KGDHC is a primary site of ROS production in normally functioning mitochondria...
  91. pmc Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants
    Aying Zhang
    College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People s Republic of China
    Plant Physiol 141:475-87. 2006
    ..ABA-induced H(2)O(2) production activates MAPK, which in turn induces the expression and the activities of antioxidant enzymes. The activation of MAPK also enhances the H(2)O(2) production, forming a positive feedback loop...
  92. ncbi Generation of reactive oxygen species in the reaction catalyzed by alpha-ketoglutarate dehydrogenase
    Laszlo Tretter
    Department of Medical Biochemistry, Semmelweis University, and Neurochemistry Group, Hungarian Academy of Sciences, Budapest H 1444, Hungary
    J Neurosci 24:7771-8. 2004
    ..Thus alpha-KGDH is not only a target of ROS but could significantly contribute to generation of oxidative stress in the mitochondria...
  93. ncbi Effect of miR-23 on oxidant-induced injury in human retinal pigment epithelial cells
    Haijiang Lin
    Departments of Ophthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, Texas 77555, USA
    Invest Ophthalmol Vis Sci 52:6308-14. 2011
    ..The purpose of this study was to investigate the role of miR-23a in modulating RPE cell survival and gene expression in response to oxidative damage...
  94. pmc Genome-wide analysis of hydrogen peroxide-regulated gene expression in Arabidopsis reveals a high light-induced transcriptional cluster involved in anthocyanin biosynthesis
    Sandy Vanderauwera
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Belgium
    Plant Physiol 139:806-21. 2005
    In plants, reactive oxygen species and, more particularly, hydrogen peroxide (H(2)O(2)) play a dual role as toxic by-products of normal cell metabolism and as regulatory molecules in stress perception and signal transduction...
  95. ncbi A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance
    Takayuki Asano
    National Institute of Agrobiological Sciences, 2 1 2 Kannondai, Tsukuba, Ibaraki 305 8602, Japan
    Plant J 69:26-36. 2012
    ..The accumulation of hydrogen peroxide (H(2) O(2) ) in the leaves was less in OsCPK12-OX plants than in wild-type (WT) plants...
  96. pmc Hydrogen peroxide fuels aging, inflammation, cancer metabolism and metastasis: the seed and soil also needs "fertilizer"
    Michael P Lisanti
    The Jefferson Stem Cell Biology and Regenerative Medicine Center, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA
    Cell Cycle 10:2440-9. 2011
    ..In this regard, a series of recent studies have elegantly shown that the production of hydrogen peroxide, by both cancer cells and cancer-associated fibroblasts, may provide the necessary "fertilizer," by driving ..
  97. pmc Hydrogen peroxide-dependent DNA release and transfer of antibiotic resistance genes in Streptococcus gordonii
    Andreas Itzek
    Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Bacteriol 193:6912-22. 2011
  98. pmc Caloric restriction or catalase inactivation extends yeast chronological lifespan by inducing H2O2 and superoxide dismutase activity
    Ana Mesquita
    Instituto de Investigação em Ciências da Vida e Saúde, Escola de Ciências da Saúde, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Proc Natl Acad Sci U S A 107:15123-8. 2010
    ..of catalases extends chronological lifespan by inducing elevated levels of the reactive oxygen species hydrogen peroxide, which activate superoxide dismutases that inhibit the accumulation of superoxide anions...
  99. pmc Characterization of hydrogen peroxide-induced DNA release by Streptococcus sanguinis and Streptococcus gordonii
    Jens Kreth
    Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Bacteriol 191:6281-91. 2009
    ..and Streptococcus gordonii release DNA in a process induced by pyruvate oxidase-dependent production of hydrogen peroxide (H(2)O(2)). Surprisingly, S. sanguinis and S...
  100. ncbi The impact of oxidative stress on Arabidopsis mitochondria
    L J Sweetlove
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
    Plant J 32:891-904. 2002
  101. ncbi The multifaceted capacity of Dps proteins to combat bacterial stress conditions: Detoxification of iron and hydrogen peroxide and DNA binding
    Emilia Chiancone
    Department of Biochemical Sciences A Rossi Fanelli, Sapienza University of Rome, Rome, Italy
    Biochim Biophys Acta 1800:798-805. 2010
    ..The widely expressed Dps proteins, so named after the DNA-binding properties of the first characterized member of the family in Escherichia coli, are considered major players in the bacterial response to stress...

Research Grants76

  1. Anti-tumor properties of curcumin in oral cancers: role of thioredoxin reductase
    STEPHEN WILLIAM TUTTLE; Fiscal Year: 2010
    ..The electrons donated by NADPH are instead diverted to O2, to form superoxide and ultimately hydrogen peroxide. In this proposal we will investigate the role of TxRd1 as a critical mediator of the antitumorigeniceffects ..
  2. A Hybrid PARP Inhibitor and Redox Catalyst for Lung Transplantation
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..free radical superoxide anion that is subsequently converted into powerful toxins, including peroxynitrite, hydrogen peroxide, and hydroxyl radical...
  3. Peroxynitrite Decomposition Catalyst and Nitric Oxide Donor for Endotoxemia
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..of reactive oxygen species degradation: a superoxide dismutase mimetic, a catalase mimic that detoxifies hydrogen peroxide, and a peroxynitrite decomposition catalyst...
  4. Epigenetic mechanisms relevant to the pathogenesis of ALS
    Neil W Kowall; Fiscal Year: 2013
    ..of oxidative injury in neurons, we treated NSC-34 motor neuronal cells transfected with LSD1 shRNA with hydrogen peroxide and found that reduced LSD1 expression was associated with resistance to oxidative injury further supporting ..
  5. Group A Streptococcus responses to oxidative stress
    Ioannis Gryllos; Fiscal Year: 2010
    ..1) plays a role in GAS oxidative stress responses and virulence. Comparison of global responses to hydrogen peroxide (H2O2) or to H2O2 combined with human neutrophil granule contents in wild type versus perR mutant bacteria ..
  6. Vascular dysfunction and oxidative stress in primary amyloidosis
    RAYMOND QUEZON MIGRINO; Fiscal Year: 2010
    ..measure reactive oxygen species (superoxide and hydrogen peroxide) in coronary and adipose arterioles following exposure to amyloid light chain and 3...
  7. Novel Peptide MPO Inhibitors for Treating Atherosclerosis
    Kirkwood A Pritchard; Fiscal Year: 2012
    ..Upon activation these inflammatory phagocytes release MPO and begin to generate hydrogen peroxide (H2O2), which is required to activate MPO...
  8. A novel peroxidase in vascular endothelium and the development of atherosclerosis
    Guangjie Cheng; Fiscal Year: 2012
    ..VPO, when co- expressed in cells with novel vascular Noxes, utilizes hydrogen peroxide produced by these Noxes to catalyze peroxidative reactions...
  9. Human neutrophils, phospholipase A2 and S.aureus: microbial targets and responses
    William M Nauseef; Fiscal Year: 2013
    ..Importantly, hydrogen peroxide (H2O2) produced by PMN is amplified by the PMN granule protein myeloperoxidase (MPO) to generate HOCl (..
  10. Global regulatory interactions in bacterial pathogenesis
    BRIAN AKERLEY; Fiscal Year: 2009
    ..critical virulence properties including the structure of its lipooligosaccharide (LOS) and resistance to hydrogen peroxide. The outermost structure of LOS is composed of a variable set of carbohydrates and other modifications ..
  11. Role of Peroxidasin in Glomerular Matrix Homeostasis
    Gautam Bhave; Fiscal Year: 2013
    ..Superoxide and its product, hydrogen peroxide (H2O2), are ROS that have garnered greatest attention in DN...
  12. Impaired mitochondrial fusion in pulmonary arterial hypertension
    Stephen L Archer; Fiscal Year: 2010
    ..Hooded Rats (FHR), and have identified problems in the FHR's mitochondria, namely lower production of hydrogen peroxide and fragmentation of the mitochondrial network, which create a pseudohypoxic environment that favors rapid ..
  13. Alcohol-Induced Bone Resorption: The Role of Oxidative Stress
    Martin J J Ronis; Fiscal Year: 2013
    ..and loss of function studies will be performed by transient transfection and use of siRNA and the effects of hydrogen peroxide and free radical scavenging on the ERK/STAT3/RANKL cascade will be examined...
  14. Redox regulation of phagocyte NOX2 in inflammation and aging
    Robert A Clark; Fiscal Year: 2013
    ..We have recently demonstrated a novel feed-forward regulation of NOX5 by hydrogen peroxide (H2O2) acting through a signaling pathway involving calcium and the non-receptor tyrosine kinase c-Abl...
  15. Scavenging of Reactive Oxygen and Nitrogen Species by Cobalamins
    NICOLA ELIZABETH BRASCH; Fiscal Year: 2011
    ..of Reactive Oxygen and Nitrogen Species (ROS/RNS), such as nitric oxide, superoxide, the hydroxyl radical, hydrogen peroxide and peroxynitrite...
  16. Novel pathways for fat burning that protects from high fat diet-induced metabolic
    Sihem Boudina; Fiscal Year: 2013
    ..of manganese superoxide dismutase (MnSOD), the sole enzyme responsible for detoxifying superoxide to hydrogen peroxide in the mitochondrial matrix...
  17. Mitochondrial Mechanisms of Programmed Necrosis in the Aged Female Heart
    Alexandra M Machikas; Fiscal Year: 2013
    ..rate, calcium retention capacity (CRC), and fluorescent detection of superoxide, peroxynitrite, and hydrogen peroxide. The molecular basis of PN will be further analyzed using co- immunoprecipitation and co-localization ..
  18. HTS development for targeted anti-fungal small molecules
    Paul D Kaufman; Fiscal Year: 2010
    ..sensitive to genotoxic agents including DNA alkylating agents and reactive oxygen species (ROS) such as hydrogen peroxide. Notably, rtt109-/- mutant cells are much less pathogenic in a mouse model of systemic candidaisis induced ..
  19. TRP channels in regulation of vascular tone
    David X Zhang; Fiscal Year: 2013
    ..NO) in subjects without coronary artery disease (CAD), and reactive oxygen species (ROS), specifically hydrogen peroxide (H2O2) derived from the mitochondrial electron transport chain, in CAD patients...
  20. Novel therapeutics for vesicants and toxic inhaled chemicals (U54)
    Carl W White; Fiscal Year: 2013
    ..Catalytic antioxidant AEOL10150, which scavenges superoxide, hydrogen peroxide, lipid peroxides, and peroxynitrite, rescued: a) upper (nasal) and lower airway injuries by CEES;b) lower ..
  21. Mitochondrial Metabolism and ROS Regulate Lung Cancer
    Navdeep S Chandel; Fiscal Year: 2013
    ..The major form of ROS that participates in signaling in the cytosol is hydrogen peroxide (H2O2), which is generated by its conversion from superoxide (O2-) by copper-zinc superoxide dismutase (SOD1)..
  22. Androgen Receptor-JunD Complex Inhibitors to Prevent Prostate Cancer Progression
    Hirak S Basu; Fiscal Year: 2012
    ..cancer causative agents, cellular reactive oxygen species (ROS) such as superoxide, hydroxyl radical, hydrogen peroxide, etc...
  23. Targeted investigation of tissue specific oxidative stress in the etiology of ALS
    Holly Van Remmen; Fiscal Year: 2013
  24. Apple peel extract inhibits Cr(VI)-induced lung cancer
    Zhuo Zhang; Fiscal Year: 2013
    ..radical and chelation of Cr(V) by apple peel extract to make this chromium intermediate less reactive toward hydrogen peroxide(H2O2) for [unreadable]OH radical generation...
  25. DNA Repair of Endogenous Lesions in Carcinogenesis
    Rabindra Roy; Fiscal Year: 2010
    ..glycosylase(s) and/or other rate-limiting BER enzymes by assessing the effects of metals (copper and iron), hydrogen peroxide, and enals in hepatocytes of LEA and LEG rats and comparing the effects with that observed in tissues ..
  26. Novel Catalases and their Products
    Alan R Brash; Fiscal Year: 2013
    ..Catalase is renowned for its efficient reaction with hydrogen peroxide and its key role in the oxidative defense of all aerobic organisms...
  27. Redox regulation of intestinal T cells
    ALAN DAVID LEVINE; Fiscal Year: 2010
    ..proposal we will focus on reactive oxygen species (ROS) derived from incomplete reduction of oxygen, such as hydrogen peroxide, acting as second messengers and thus endogenous modulators of signal transduction emanating from the TCR...
  28. Molecular Imaging Probes for Reporting on Vascular Oxidative Response
    Alexei A Bogdanov; Fiscal Year: 2013
    ..MPO activity in vascular lesions is rate-limited by the rates of local hydrogen peroxide production...
  29. Angiotensin-induced Oxidants and Vascular Cell Phenotype
    Richard Cohen; Fiscal Year: 2009
    ..In addition, in the smooth muscle, hydrogen peroxide (H2O2) formed by dismutation of NADPH oxidase-derived superoxide (O2) is an important ROS that mediates ..
  30. NADPH-Oxidase and SLE Susceptibility
    Swapan K Nath; Fiscal Year: 2013
    ..Reactive oxygen species (ROS), such as superoxide and hydrogen peroxide are key for defense against invading microbial pathogens, and are produced by the multi-protein NADPH- ..
  31. Role of Polyamine Oxidase in Apoptosis Studies with RNA Interference
    GLENN KUEHN; Fiscal Year: 2009
    ..Polyamine oxidase (PAO) catalyzes oxygen-dependent cleavage of the polyamines to form hydrogen peroxide (H2O2), among other products...
  32. Plasmalogen-derived bioactive lipids in atherosclerosis
    David A Ford; Fiscal Year: 2013
    ..Upon activation, monocytes and macrophages release MPO and produce its substrate, hydrogen peroxide, resulting in MPO-catalyzed production of the reactive chlorinating species (RCS), hypochlorous acid...
  33. Ca2+ mishandling and ischemia-vulnerability in fALS model motor terminals
    Gavriel David; Fiscal Year: 2012
    ..stimulation of motor nerves, energy stresses (ischemia/reperfusion, oxygen/glucose deprivation) and hydrogen peroxide. We hypothesize that mitochondria of fALS mouse terminals eventually lose their capacity to increase ..
    Joseph J Cullen; Fiscal Year: 2013
    ..normal cells. We have firmly established that pharmacological ascorbate is a pro-drug for delivery of hydrogen peroxide (H2O2) in vitro and in vivo via its autoxidation...
  35. Hydrogen Peroxide and Flow-Induced Dilation of Human Coronary Microcirculation
    David D Gutterman; Fiscal Year: 2012
    ..through a novel mechanism involving endothelial production of reactive oxygen species (ROS) including hydrogen peroxide (H2O2). Surprisingly the mitochondrial respiratory chain plays a necessary role in FMD in the human heart...
  36. Respiratory and Metabolic Adaptation to Hypoxia
    Paul T Schumacker; Fiscal Year: 2013
    ..Collectively, these studies will provide new information regarding the mechanisms of oxygen sensing in cells, which is important in normal tissues and in disease states. ..
  37. Studies toward the development of monoamine oxidase B specific inhibitors
    ERIKA MARIE MILCZEK; Fiscal Year: 2010
    ..that the age-related increase in MAO B levels in neuronal tissue together with the catalytic production of hydrogen peroxide (leading to reactive oxygen species) contributes to cellular apoptosis and subsequent neurodegeneration...
  38. Catalase therapy for cardiac regeneration
    Michael E Davis; Fiscal Year: 2013
    ..following infarction not only increases damage to the local myocardium, but through dismutation to hydrogen peroxide may increase lipid peroxidation and cardiac fibrosis...
  39. Preventing Human Prion Diseases - Inactivation of Prions
    Stanley B Prusiner; Fiscal Year: 2010
    ..denaturants that are more efficacious than acidic SDS, and adjuncts to acidic SDS treatment such as hydrogen peroxide, which may be as effective at lower temperatures...
  40. Mechanistic Studies of Alkyl Hydroperoxide Reductase and Related Redox Proteins
    Leslie B Poole; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Hydrogen peroxide is a toxin used by the human immune system to kill infectious organisms, and increasing evidence shows that it is also a common second messenger in eukaryotic signaling...
  41. The Electrochemistry of Diheme Cytochrome c Peroxidases
    Sean J Elliott; Fiscal Year: 2010
    ..Bacterial organisms, like all organisms, destroy toxic hydrogen peroxide by the use of specific enzymes...
  42. RNA Modifications as Biomarkers of Environmental Stress and Inflammation
    Thomas J Begley; Fiscal Year: 2013
    ..after exposure of human HEK293 cells to the DNA damaging agents methyl methanesulfonate (MMS) and hydrogen peroxide (H2O2)...
  43. Oxidative activation of Src in smoke-induced epithelial mesenchymal transition
    Henry Jay Forman; Fiscal Year: 2013
    ..Aim 1 is to test the hypothesis that CSE and two CSE components, hydrogen peroxide (H2O2) and acrolein, initiate Src activation through oxidation or alkylation of regulatory cysteine residues...
  44. Engineered tumor-specific T cells Resist Hypoxic-Tumor Immunosuppressive Attacks.
    Patricia Yotnda; Fiscal Year: 2013
    ..Most tumors also constitutively express large quantities of reactive oxygen species (ROS), including hydrogen peroxide (H2O2), that promote their proliferation and further inhibit immune responses...
  45. Preventing Burn Wound Infection Using a Novel Free Radical Sterilization System
    BENJAMIN DAVID SADOWITZ; Fiscal Year: 2011
    ..developed a totally novel free radical sterilization system by combining non-thermal plasma processing with hydrogen peroxide and ozone technologies...
  46. Reactive oxygen species in Ni(II) carcinogenesis
    Xianglin Shi; Fiscal Year: 2012
    ..to activate NADPH oxidase via cdc42 and p47phox to produce superoxide radical, which is then converted to hydrogen peroxide and hydroxyl radical...
  47. A New Low Power Low Cost Ozone Sterilizer for Medical Equipment
    Joseph R Stetter; Fiscal Year: 2012
    ..Medical sterilizers available on the market today are primarily steam-based, with ethylene oxide and hydrogen peroxide plasma as other alternatives...
  48. Spectroscopic Studies of Active Sites in Copper Proteins
    Edward I Solomon; Fiscal Year: 2011
    ..abstract_text> ..
  49. The Use of 2-Deoxyglucose in Head and Neck Cancer Therapy
    DOUGLAS ROBERT SPITZ; Fiscal Year: 2013
  50. Bifunctional Nitric Oxide Donor Refractory to Nitrate Tolerance
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..reactive oxygen species degradation: a superoxide dismutase (SOD) mimetic, a catalase mimic that detoxifies hydrogen peroxide, and a peroxynitrite decomposition catalyst...
  51. ecSOD derived peroxide in pulmonary adaptation to hypoxia
    Michael S Wolin; Fiscal Year: 2013
    ..expression of pulmonary hypertension resulting from exposure to chronic hypoxia through the generation of hydrogen peroxide and its stimulation of cGMP protein kinase (PKG)...
  52. Oxidant Mechanisms in Neonatal Brain Injury
    Donna M Ferriero; Fiscal Year: 2013
    ..We have shown that hydrogen peroxide toxicity is related to cell death but a paradox exists such that H2O2 may also be a signaling molecule that ..
  53. Perinatal regulation of endothelial NOS
    Stephen M Black; Fiscal Year: 2010
    ..ET-1 inhibits eNOS activity: decreasing ser1177 phosphorylation secondary to a catalase-mediated decrease in hydrogen peroxide (H2O2) that is regulated by PKCd activation...
  54. Androgen Modulation of Neurodegeneration in Dopamine Neurons
    Rebecca L Cunningham; Fiscal Year: 2010
    ..apoptotic signaling pathways activated by androgens in dopaminergic N27 cells following oxidative stress (hydrogen peroxide)...
  55. Estrogen and EDHF in NO Deficiency
    An Huang; Fiscal Year: 2012
    ..In addition, as a consequence of activation of CYP, the impact of superoxide/hydrogen peroxide on estrogen- and shear stress-specific signaling cascades will be evaluated...
  56. Investigating the Import Mechanism of Folded Proteins into Peroxisomes
    Angelyn Larkin; Fiscal Year: 2013
    ..Along with mitochondria, these organelles segregate toxic reactive oxygen species, such as hydrogen peroxide and superoxide anions, from the rest of the cell to prevent damage to proteins and nucleic acids associated ..
  57. Regulation of Vascular Smooth Muscle Calcium by NADPH Redox
    Sachin A Gupte; Fiscal Year: 2012
    ..protein inactivates the channel and whether changes in the levels of reduced/oxidized glutathione (GSH) or hydrogen peroxide (H2O2), induced by decrease in NADPH levels (due to the inhibition of G6PD activity), modulates L-type Ca2+ ..
  58. Vascular Smooth Muscle Function in Pulmonary Hypertension
    Nikki L Jernigan; Fiscal Year: 2013
    ..3) Examine the effect of cellular redox potential on ASIC1 activation. We hypothesize that decreased hydrogen peroxide (H2O2) following CH increases ASIC1 surface expression and channel activity...
  59. p38 MAPK a regulator of muscle contraction and function
    Zhen Yan; Fiscal Year: 2013
    ..In cultured myotubes, we and others have shown that atrophy induced by the oxidant hydrogen peroxide could be blocked by inhibitors to p381/p382, resulting in attenuated induction of E3 ubiquitin ligases and ..
  60. The role of peroxidasin in Goodpasture's disease
    ABRAHAM SCOTT MCCALL; Fiscal Year: 2013
    ..This bond is formed by the recently identified enzyme peroxidasin (PXDN) which putatively utilizes hydrogen peroxide as an initial oxidant to form hypobromous acid (HOBr) as the terminal oxidant for S=N bond formation...
  61. Regulation of PMCA Pump-Channels by Oxidant Stress
    William P Schilling; Fiscal Year: 2010
    ..abstract_text> ..
  62. Omics-Based Assays for Screening Chemical Toxicity Using Stem Cells
    Daojing Wang; Fiscal Year: 2013
    ..studies of their responses to genotoxic stresses such as ionizing radiation and chemical toxicants such as hydrogen peroxide. In this Phase I project, we will demonstrate a robust and universal two-stage workflow for Omics-based and ..
  63. Bacterial Response to Singlet Oxygen
    Timothy Donohue; Fiscal Year: 2009
    ..When single electrons are transferred to O2, it is reduced to superoxide, hydrogen peroxide, or hydroxyl radicals. When energy is transferred to O2, singlet oxygen ([1]O2) is formed...
    Sudhir Shah; Fiscal Year: 1993
    ..Specific Aim: A. To examine intracellular generation of hydrogen peroxide by normal glomeruli and by glomeruli in response to anti-Fx1A antibody...
    David Eschenbach; Fiscal Year: 1999
    ..upon three components: normal vaginal flora which inhibits microorganisms by producing a low pH and hydrogen peroxide, reducing the attachment of bacteria to the vaginal epithelium which reduces the ability of the bacteria to ..
  66. Mitochondrial SOD & Breast Cancer Risk-Mechanism
    JAMES YAGER; Fiscal Year: 2003
    ..1) Determine the relationship between mitochondrial MnSOD protein levels, MnSOD activity, and superoxide and hydrogen peroxide production...
    SPENCER FARR; Fiscal Year: 1993
    ..application) Normal aerobic metabolism produces reactive oxygen species including superoxide anions, hydrogen peroxide and hydroxyl radicals...
    Julie Andersen; Fiscal Year: 1999
    Superoxide and hydrogen peroxide are produced during normal metabolism...
  69. Dense Medium Plasma Remediation of VOCs in Groundwater
    Yonghui Ma; Fiscal Year: 2007
    ..generated by the submerged micro- discharges produce a variety of reactive species including ozone (O3), hydrogen peroxide (H2O2), and free radicals (e.g...
    b>Hydrogen peroxide has been found in the aqueous humor of several animal species including man, at the low uM level (5 to 90 uM)...
    EDWIN THOMAS; Fiscal Year: 1992
    Lactoperoxidase (LP), hydrogen peroxide (H2O2) and thiocyanate ion (SCN-) form a bacteriostatic system in human saliva. The oral "lactic-acid bacteria" release H2O2 as a by-product of carbohydrate metabolism...
    GARY PIELAK; Fiscal Year: 2001
    ..The proposed in vitro studies focus on the reactive oxygen species, hydrogen peroxide, and two proteins found in Lewy bodies, cytochrome C and alpha-synuclein...
  73. Dosimetry of Lipid-Derived Amino Acid Adducts by LC/MS
    Ian Blair; Fiscal Year: 2006
    ..These studies will serve as a precursor to future dosimetry studies in large populations and will provide insight into the role of lipid peroxidation as a mediator of carcinogenesis. ..
  74. Development of a Live Combinational Microbicide for Women
    Qiang Xu; Fiscal Year: 2010
    ..Both UTIs and BV are associated with a depletion of hydrogen peroxide (H2O2)-producing lactobacilli that normally protect the vagina from infection by opportunistic pathogens...
    Michael S Wolin; Fiscal Year: 2010
    This proposal focuses on defining the role of Nox oxidase-derived hydrogen peroxide, and cytosolic NADPH and NADH redox systems in processes that control signaling mechanisms regulating vascular smooth muscle force responses to ..
    Y Kang; Fiscal Year: 1999
    ..Catalase is a major enzyme involved in detoxification of hydrogen peroxide (H2O2) in mammalian cells...