Summary: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).

Top Publications

  1. pmc Matrix crosslinking forces tumor progression by enhancing integrin signaling
    Kandice R Levental
    Department of Bioengineering and Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cell 139:891-906. 2009
  2. ncbi Riboflavin/ultraviolet-a-induced collagen crosslinking for the treatment of keratoconus
    Gregor Wollensak
    Department of Ophthalmology, Technical University of Dresden, Germany
    Am J Ophthalmol 135:620-7. 2003
  3. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
  4. ncbi Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
  5. pmc MicroRNA-192 in diabetic kidney glomeruli and its function in TGF-beta-induced collagen expression via inhibition of E-box repressors
    Mitsuo Kato
    Gonda Diabetes Center, Division of Molecular Biology, Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 104:3432-7. 2007
  6. pmc Platelets amplify inflammation in arthritis via collagen-dependent microparticle production
    Eric Boilard
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Science 327:580-3. 2010
  7. pmc Aligned collagen is a prognostic signature for survival in human breast carcinoma
    Matthew W Conklin
    Department of Pharmacology, and the Laboratory for Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Am J Pathol 178:1221-32. 2011
  8. doi The enhancement of cancer stem cell properties of MCF-7 cells in 3D collagen scaffolds for modeling of cancer and anti-cancer drugs
    Lei Chen
    State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, PR China
    Biomaterials 33:1437-44. 2012
  9. pmc Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis
    Katarina Wolf
    Department of Dermatology, University of Wurzburg, 97080 Wurzburg, Germany
    J Cell Biol 160:267-77. 2003
  10. ncbi Multi-step pericellular proteolysis controls the transition from individual to collective cancer cell invasion
    Katarina Wolf
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine and Department of Dermatology, University of Wurzburg, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    Nat Cell Biol 9:893-904. 2007

Detail Information

Publications407 found, 100 shown here

  1. pmc Matrix crosslinking forces tumor progression by enhancing integrin signaling
    Kandice R Levental
    Department of Bioengineering and Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cell 139:891-906. 2009
    ..We found that breast tumorigenesis is accompanied by collagen crosslinking, ECM stiffening, and increased focal adhesions...
  2. ncbi Riboflavin/ultraviolet-a-induced collagen crosslinking for the treatment of keratoconus
    Gregor Wollensak
    Department of Ophthalmology, Technical University of Dresden, Germany
    Am J Ophthalmol 135:620-7. 2003
    In animal eyes, a significant increase in corneal biomechanical stiffness has been found after collagen crosslinking by combined riboflavin/ultraviolet-A (UVA) treatment...
  3. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
  4. ncbi Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
    ..cultured on surfaces with stiffness ranging from 2 to 55,000 Pa that have been laminated with fibronectin or collagen as adhesive ligand...
  5. pmc MicroRNA-192 in diabetic kidney glomeruli and its function in TGF-beta-induced collagen expression via inhibition of E-box repressors
    Mitsuo Kato
    Gonda Diabetes Center, Division of Molecular Biology, Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 104:3432-7. 2007
    Key features of diabetic nephropathy (DN) include the accumulation of extracellular matrix proteins such as collagen 1-alpha 1 and -2 (Col1a1 and -2)...
  6. pmc Platelets amplify inflammation in arthritis via collagen-dependent microparticle production
    Eric Boilard
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Science 327:580-3. 2010
    ..Using both pharmacologic and genetic approaches, we identified the collagen receptor glycoprotein VI as a key trigger for platelet microparticle generation in arthritis pathophysiology...
  7. pmc Aligned collagen is a prognostic signature for survival in human breast carcinoma
    Matthew W Conklin
    Department of Pharmacology, and the Laboratory for Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Am J Pathol 178:1221-32. 2011
    ..We have performed a rigorous examination of the relationship of a tumor-associated collagen signature-3 (TACS-3) to the long-term survival rate of human patients...
  8. doi The enhancement of cancer stem cell properties of MCF-7 cells in 3D collagen scaffolds for modeling of cancer and anti-cancer drugs
    Lei Chen
    State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, PR China
    Biomaterials 33:1437-44. 2012
    Three-dimensional (3D) culture could partially simulate in vivo conditions. In this work, we developed a 3D collagen scaffold to investigate cellular properties of MCF-7 cells...
  9. pmc Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis
    Katarina Wolf
    Department of Dermatology, University of Wurzburg, 97080 Wurzburg, Germany
    J Cell Biol 160:267-77. 2003
    ..In three-dimensional collagen matrices, spindle-shaped proteolytically potent HT-1080 fibrosarcoma and MDA-MB-231 carcinoma cells exhibited a ..
  10. ncbi Multi-step pericellular proteolysis controls the transition from individual to collective cancer cell invasion
    Katarina Wolf
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine and Department of Dermatology, University of Wurzburg, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    Nat Cell Biol 9:893-904. 2007 invasive HT-1080 fibrosarcoma and MDA-MB-231 breast cancer cells coordinate mechanotransduction and fibrillar collagen remodelling by segregating the anterior force-generating leading edge containing beta1 integrin, MT1-MMP and F-..
  11. pmc Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
    Warren R Zipfel
    Department of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 100:7075-80. 2003
    ..of intrinsic indicators within tissue, such as nicotinamide adenine dinucleotide, retinol, indoleamines, and collagen provides crucial information for physiology and pathology...
  12. pmc Protease-dependent versus -independent cancer cell invasion programs: three-dimensional amoeboid movement revisited
    Farideh Sabeh
    Division of Molecular Medicine and Genetics, Department of Internal Medicine, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    J Cell Biol 185:11-9. 2009
    ..metastasis requires cancer cells to negotiate a stromal environment dominated by cross-linked networks of type I collagen. Although cancer cells are known to use proteinases to sever collagen networks and thus ease their passage ..
  13. doi Comparison of sequential vs same-day simultaneous collagen cross-linking and topography-guided PRK for treatment of keratoconus
    Anastasios John Kanellopoulos
    Laservision gr Institute, Athens, Greece New York University Medical College and Manhattan Eye, Ear and Throat Hospital, New York, NY, USA
    J Refract Surg 25:S812-8. 2009
    The safety and efficacy of corneal collagen cross-linking (CXL) and topography-guided photorefractive keratectomy (PRK) using a different sequence and timing were evaluated in consecutive keratoconus cases.
  14. pmc Integrins
    Malgorzata Barczyk
    Department of Biomedicine, University of Bergen, Norway
    Cell Tissue Res 339:269-80. 2010
    ..In some ECM molecules, such as collagen and certain laminin isoforms, the RGD sequences are exposed upon denaturation or proteolytic cleavage, allowing ..
  15. doi Myocardial remodeling after infarction: the role of myofibroblasts
    Susanne W M van den Borne
    Cardiovascular Research Institute Maastricht, Department of Pharmacology and Toxicology and Cardiology, Maastricht University, Maastricht, The Netherlands
    Nat Rev Cardiol 7:30-7. 2010 no longer considered to be passive tissue; it is an active playground where myofibroblasts play a role in collagen turnover and scar contraction...
  16. pmc Suppression of microRNA-29 expression by TGF-β1 promotes collagen expression and renal fibrosis
    Bo Wang
    JDRF Danielle Alberti Memorial Centre for Diabetes Complications, Diabetes Division, Baker IDI Heart and Diabetes Institute, Melbourne, Australia
    J Am Soc Nephrol 23:252-65. 2012
    ..tubular cells, primary mesangial cells, and podocytes to TGF-β1 to examine its effect on miRNAs and subsequent collagen synthesis...
  17. pmc Collagen prolyl hydroxylases are essential for breast cancer metastasis
    Daniele M Gilkes
    Vascular Program, Institute for Cell Engineering McKusick Nathans Institute of Genetic Medicine, The Johns Hopkins University, Baltimore, Maryland, USA
    Cancer Res 73:3285-96. 2013
    ..In this study, we demonstrate that hypoxia-inducible factor 1 activates the transcription of genes encoding collagen prolyl hydroxylases that are critical for collagen deposition by breast cancer cells...
  18. ncbi Matrigel: basement membrane matrix with biological activity
    Hynda K Kleinman
    NIH, NIDCR, 30 433, 30 Convent Dr MSC 4370, Bethesda, MD 20892 4370, USA
    Semin Cancer Biol 15:378-86. 2005
    ..In vivo, it is used for measuring angiogenic inhibitors and stimulators, to improve graft survival, repair damaged tissues, and increase tumor growth...
  19. ncbi Rac1 orientates epithelial apical polarity through effects on basolateral laminin assembly
    T S Jou
    Department of Anatomy, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Nat Cell Biol 3:831-8. 2001
    ..These findings implicate Rac1-mediated laminin assembly in apical pole orientation. By linking apical orientation to generation of the basement membrane, epithelial cells ensure the coordination of polarity with tissue architecture...
  20. pmc Collagen fibrillogenesis: fibronectin, integrins, and minor collagens as organizers and nucleators
    Karl E Kadler
    Wellcome Trust Centre for Cell Matrix Research, University of Manchester, Faculty of Life Sciences, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom
    Curr Opin Cell Biol 20:495-501. 2008
    ..There are more than 30 collagens and collagen-related proteins but the most abundant are collagens I and II that exist as D-periodic (where D = 67 nm) fibrils...
  21. pmc MMP-9/gelatinase B is a key regulator of growth plate angiogenesis and apoptosis of hypertrophic chondrocytes
    T H Vu
    Department of Anatomy, University of California, San Francisco 94143, USA
    Cell 93:411-22. 1998
    ..Growth plates from gelatinase B-null mice in culture show a delayed release of an angiogenic activator, establishing a role for this proteinase in controlling angiogenesis...
  22. pmc Targeted disruption of decorin leads to abnormal collagen fibril morphology and skin fragility
    K G Danielson
    Department of Pathology, Anatomy, and Cell Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Cell Biol 136:729-43. 1997
    ..Ultrastructural analysis revealed abnormal collagen morphology in skin and tendon, with coarser and irregular fiber outlines...
  23. ncbi Structural basis of collagen recognition by integrin alpha2beta1
    J Emsley
    Department of Biochemistry, University of Leicester, United Kingdom
    Cell 101:47-56. 2000
    ..determined the crystal structure of a complex between the I domain of integrin alpha2beta1 and a triple helical collagen peptide containing a critical GFOGER motif...
  24. doi Degradation of fibrinogen and collagen by staphopains, cysteine proteases released from Staphylococcus aureus
    Takehisa Ohbayashi
    Department of Oral and Maxillo Facial Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan
    Microbiology 157:786-92. 2011
    ..activities of staphopains pertinent to clotting disorders and tissue destruction, we examined their effects on collagen, one of the major tissue components, and on plasma clotting...
  25. doi Collagen cross-links as a determinant of bone quality: a possible explanation for bone fragility in aging, osteoporosis, and diabetes mellitus
    M Saito
    Department of Orthopaedic Surgery, Jikei University School of Medicine, 3 25 8 Nishi shinbashi, Minato ku, Tokyo 105 8461, Japan
    Osteoporos Int 21:195-214. 2010
    b>Collagen cross-linking, a major post-translational modification of collagen, plays important roles in the biological and biomechanical features of bone...
  26. pmc Collagen reorganization at the tumor-stromal interface facilitates local invasion
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI, USA
    BMC Med 4:38. 2006
    ..Moreover, collagen-dense breast tissue increases the risk of breast carcinoma, although the relationship between collagen density ..
  27. pmc Human umbilical cord mesenchymal stem cells reduce fibrosis of bleomycin-induced lung injury
    Yuben Moodley
    Department of Medicine, Monash Immunology and Stem Cell Laboratories, School of Biomedical Sciences, Monash University, Melbourne, Australia
    Am J Pathol 175:303-13. 2009
    ..b>Collagen concentration in the lung was significantly reduced by uMSC treatment, which may have been a consequence of the ..
  28. pmc The actin-bundling protein fascin stabilizes actin in invadopodia and potentiates protrusive invasion
    Ang Li
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland, UK
    Curr Biol 20:339-45. 2010
    ..We further implicate fascin in invasive migration into collagen I-Matrigel gels and particularly in cell types that use an elongated mesenchymal type of motility in 3D...
  29. doi Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin
    Stefan R Braam
    Leiden University Medical Centre, Postal Zone S 1 P, P O Box 9600, 2300 RC Leiden, The Netherlands
    Stem Cells 26:2257-65. 2008
    ..attached efficiently to natural human vitronectin, fibronectin, and Matrigel but poorly to laminin + entactin and collagen IV...
  30. ncbi Use of mesenchymal stem cells in a collagen matrix for Achilles tendon repair
    R G Young
    Osiris Therapeutics, Cleveland, Ohio, USA
    J Orthop Res 16:406-13. 1998
    ..Cultured, autologous, marrow-derived mesenchymal stem cells were suspended in a collagen gel delivery vehicle; the cell-gel composite was subsequently contracted onto a pretensioned suture...
  31. pmc Salivary antigen-5/CAP family members are Cu2+-dependent antioxidant enzymes that scavenge O₂₋. and inhibit collagen-induced platelet aggregation and neutrophil oxidative burst
    Teresa C F Assumpção
    Vector Biology Section, Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 288:14341-61. 2013
    ..and discovered to attenuate platelet aggregation, ATP secretion, and thromboxane A2 generation by low doses of collagen (<1 μg/ml) but no other agonists. DMAV did not interact with collagen, glycoprotein VI, or integrin α2β1...
  32. pmc Targeted deletion of matrix metalloproteinase-9 attenuates left ventricular enlargement and collagen accumulation after experimental myocardial infarction
    A Ducharme
    Cardiovascular Division, Department of Medicine, and Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 106:55-62. 2000
    ..MMP-9 KO mice had less collagen accumulation in the infarcted area than did WT mice, and they showed enhanced expression of MMP-2, MMP-13, and ..
  33. pmc Structural insights into triple-helical collagen cleavage by matrix metalloproteinase 1
    Szymon W Manka
    Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Kennedy Institute of Rheumatology, University of Oxford, London W6 8LH, United Kingdom
    Proc Natl Acad Sci U S A 109:12461-6. 2012 major roles in morphogenesis, tissue repair, and human diseases, but how they recognize and cleave the collagen triple helix is not fully understood...
  34. pmc Interleukin 6 mediates myocardial fibrosis, concentric hypertrophy, and diastolic dysfunction in rats
    Giselle C Melendez
    Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, SC 29208, USA
    Hypertension 56:225-31. 2010
    ..Myocardial interstitial collagen volume fraction and isolated cardiomyocyte size were also assessed...
  35. pmc Collagen fibrils: nanoscale ropes
    Laurent Bozec
    Bone and Mineral Centre, Department of Medicine, and Department of Civil and Environmental Engineering, University College London, London, England
    Biophys J 92:70-5. 2007
    The formation of collagen fibrils from staggered repeats of individual molecules has become "accepted" wisdom. However, for over thirty years now, such a model has failed to resolve several structural and functional questions...
  36. ncbi Collagens--structure, function, and biosynthesis
    K Gelse
    Cartilage Research, Department of Pathology, University of Erlangen Nurnberg, Krankenhausstr 8 10, D 91054 Erlangen, Germany
    Adv Drug Deliv Rev 55:1531-46. 2003
    ..The most abundant family is the collagens with more than 20 different collagen types identified so far...
  37. pmc Collagen-based cell migration models in vitro and in vivo
    Katarina Wolf
    Department of Cell Biology, Nijmegen Center for Molecular Life Science, Radboud University Nijmegen, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Semin Cell Dev Biol 20:931-41. 2009
    Fibrillar collagen is the most abundant extracellular matrix (ECM) constituent which maintains the structure of most interstitial tissues and organs, including skin, gut, and breast...
  38. pmc Physiological levels of tumstatin, a fragment of collagen IV alpha3 chain, are generated by MMP-9 proteolysis and suppress angiogenesis via alphaV beta3 integrin
    Yuki Hamano
    Center for Matrix Biology, Department of Medicine and Gastroenterology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Cancer Cell 3:589-601. 2003
    We demonstrate a physiological role for tumstatin, a cleavage fragment of the alpha3 chain of type IV collagen (Col IValpha3), which is present in the circulation...
  39. ncbi Collagens, modifying enzymes and their mutations in humans, flies and worms
    Johanna Myllyharju
    Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, University of Oulu, FIN 90014 Oulu, Finland
    Trends Genet 20:33-43. 2004
    Collagens and proteins with collagen-like domains form large superfamilies in various species, and the numbers of known family members are increasing constantly...
  40. ncbi Collagen-based implants reinforced by chitin fibres in a goat shank bone defect model
    Xiaoming Li
    Key Laboratory of Advanced Materials, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
    Biomaterials 27:1917-23. 2006
    ..this study, we used a goat shank model to evaluate the bone-regenerating efficacy of a novel nano-hydroxyapatite/collagen/PLLA (nHACP) composite reinforced by chitin fibres...
  41. pmc Losartan inhibits collagen I synthesis and improves the distribution and efficacy of nanotherapeutics in tumors
    Benjamin Diop-Frimpong
    Department of Radiation Oncology, Edwin L Steele Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 108:2909-14. 2011
    The dense collagen network in tumors significantly reduces the penetration and efficacy of nanotherapeutics...
  42. pmc The collagen family
    Sylvie Ricard-Blum
    Institut de Biologie et Chimie des Proteines, UMR 5086 CNRS, Universite Lyon 1, Lyon, 69367, France
    Cold Spring Harb Perspect Biol 3:a004978. 2011
    Collagens are the most abundant proteins in mammals. The collagen family comprises 28 members that contain at least one triple-helical domain...
  43. pmc A role for fibrillar collagen deposition and the collagen internalization receptor endo180 in glioma invasion
    Ivo J Huijbers
    Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom
    PLoS ONE 5:e9808. 2010
    ..In GBMs, surprisingly little is known about the associated matrix components, in particular the fibrillar proteins such as collagens that are known to play a key role in the invasion of other tumor types...
  44. ncbi Type I macrophage scavenger receptor contains alpha-helical and collagen-like coiled coils
    T Kodama
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Nature 343:531-5. 1990
    ..This stalk structure, composed of an alpha-helical coiled coil and a collagen-like triple helix, has not previously been observed in an integral membrane protein.
  45. pmc Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences
    C C Mello
    Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138
    EMBO J 10:3959-70. 1991
    ..Integration of the injected DNA, though relatively rare, was reproducibly achieved when single-stranded oligonucleotide was co-injected with the double-stranded DNA...
  46. pmc Inhibition of VEGF receptors causes lung cell apoptosis and emphysema
    Y Kasahara
    Division of Pulmonary Sciences and Critical Care Medicine, Pulmonary Hypertension Center, Department of Medicine, University of Colorado Health Sciences Center, Denver, USA
    J Clin Invest 106:1311-9. 2000
    ..These findings suggest that VEGF receptor signaling is required for maintenance of the alveolar structures and, further, that alveolar septal cell apoptosis contributes to the pathogenesis of emphysema...
  47. pmc The non-phagocytic route of collagen uptake: a distinct degradation pathway
    Daniel H Madsen
    Finsen Laboratory BRIC, Rigshospitalet, DK 2200 Copenhagen, Denmark
    J Biol Chem 286:26996-7010. 2011
    ..An important turnover pathway involves cellular internalization and degradation of large, soluble collagen fragments, generated by initial cleavage of the insoluble collagen fibers...
  48. pmc A self-propagating matrix metalloprotease-9 (MMP-9) dependent cycle of chronic neutrophilic inflammation
    Xin Xu
    Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States of America
    PLoS ONE 6:e15781. 2011
    ..Ac-PGP) as an important neutrophil (PMN) chemoattractant in chronic inflammation generated from the breakdown of collagen by the actions of MMP-9...
  49. doi Long-term results of riboflavin ultraviolet a corneal collagen cross-linking for keratoconus in Italy: the Siena eye cross study
    Aldo Caporossi
    Department of Ophthalmology and Neurosurgery, Santa Maria delle Scotte Hospital, Siena University, Siena, Italy
    Am J Ophthalmol 149:585-93. 2010
    To report the long-term results of 44 keratoconic eyes treated by combined riboflavin ultraviolet A collagen cross-linking in the first Italian open, nonrandomized phase II clinical trial, the Siena Eye Cross Study.
  50. pmc Perlecan maintains the integrity of cartilage and some basement membranes
    M Costell
    Department of Experimental Pathology, Lund University, S 221 85 Lund, Sweden
    J Cell Biol 147:1109-22. 1999
    ..The chondrodysplasia is characterized by a reduction of the fibrillar collagen network, shortened collagen fibers, and elevated expression of cartilage extracellular matrix genes, suggesting ..
  51. pmc D-2-hydroxyglutarate produced by mutant IDH1 perturbs collagen maturation and basement membrane function
    Masato Sasaki
    The Campbell Family Institute for Breast Cancer Research, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada
    Genes Dev 26:2038-49. 2012
    ..Intriguingly, D2HG also blocks prolyl-hydroxylation of collagen, causing a defect in collagen protein maturation...
  52. pmc A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene
    V Lefebvre
    Department of Molecular Genetics, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Box 11, Houston, TX 77030, USA
    EMBO J 17:5718-33. 1998
    ..Our data suggest that L-Sox5/Sox6 and Sox9, which belong to two different classes of Sox transcription factors, cooperate with each other in expression of Col2a1 and possibly other genes of the chondrocytic program...
  53. pmc Alpha-smooth muscle actin expression upregulates fibroblast contractile activity
    B Hinz
    Department of Pathology, CMU, University of Geneva, 1211 Geneva 4, Switzerland
    Mol Biol Cell 12:2730-41. 2001
    ..In a second approach, we measured the isotonic contraction of SCF- and LF-populated attached collagen lattices. SCFs exhibited 41% diameter reduction compared with 63% by LFs...
  54. doi Attenuation of transforming growth factor-β-stimulated collagen production in fibroblasts by quercetin-induced heme oxygenase-1
    Toshinobu Nakamura
    Department of Respiratory Medicine, Graduate School of Medicine, Nagoya University, Japan
    Am J Respir Cell Mol Biol 44:614-20. 2011
    ..We hypothesized that quercetin suppresses the production of collagen in fibroblasts via the induction of HO-1...
  55. pmc Discoidin domain receptors: unique receptor tyrosine kinases in collagen-mediated signaling
    Hsueh Liang Fu
    Department of Pathology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 288:7430-7. 2013
    ..DDRs regulate cell-collagen interactions in normal and pathological conditions and thus are emerging as major sensors of collagen matrices ..
  56. pmc LOX-mediated collagen crosslinking is responsible for fibrosis-enhanced metastasis
    Thomas R Cox
    Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark
    Cancer Res 73:1721-32. 2013
    ..We show that LOX-dependent collagen crosslinking is involved in creating a growth-permissive fibrotic microenvironment capable of supporting ..
  57. doi Lysine hydroxylation and cross-linking of collagen
    Mitsuo Yamauchi
    Dental Research Center, University of North Carolina, Chapel Hill, NC, USA
    Methods Mol Biol 446:95-108. 2008
    ..Hydroxylation of specific lysine residues is one of such unique modifications found in collagen, and the pattern/extent of this modification influences fibrillogenesis, cross-linking, and matrix mineralization...
  58. pmc Dynamic interplay between the collagen scaffold and tumor evolution
    Mikala Egeblad
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Curr Opin Cell Biol 22:697-706. 2010
    ..Here we discuss how abnormal expression, proteolysis and structure of these collagens influence cellular functions to elicit multiple effects on tumors, including proliferation, initiation, invasion, metastasis, and therapy response...
  59. pmc Myocardin-related transcription factor-a controls myofibroblast activation and fibrosis in response to myocardial infarction
    Eric M Small
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Circ Res 107:294-304. 2010
    ..Myocardin-related transcription factors (MRTFs) are serum response factor (SRF) cofactors that promote a smooth muscle phenotype and are emerging as components of stress-responsive signaling...
  60. pmc Mast cell tryptase stimulates the synthesis of type I collagen in human lung fibroblasts
    J A Cairns
    Immunopharmacology Group, Southampton General Hospital, United Kingdom
    J Clin Invest 99:1313-21. 1997
    ..a characteristic feature of chronic inflammation, a condition that may lead to fibrosis as a result of increased collagen synthesis by fibroblasts...
  61. ncbi Cell interactions with three-dimensional matrices
    Edna Cukierman
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 4370, USA
    Curr Opin Cell Biol 14:633-9. 2002
    ..In this review, we examine recent advances in studies of interactions of fibroblasts with collagen gels and fibronectin-containing matrices that mimic in vivo three-dimensional microenvironments...
  62. ncbi Circulating fibrocytes: collagen-secreting cells of the peripheral blood
    Timothy E Quan
    Department of Medicine and Dermatology, Section of Rheumatology, Yale University School of Medicine, P O Box 208031, 300 Cedar Street, TAC S525, New Haven, CT 06520 8031, USA
    Int J Biochem Cell Biol 36:598-606. 2004
    ..Ongoing research is focusing on the molecular signals that influence fibrocyte migration, proliferation, and function in the context of normal physiology and pathology...
  63. pmc Reduced collagen deposition in infarcted myocardium facilitates induced pluripotent stem cell engraftment and angiomyogenesis for improvement of left ventricular function
    Bo Dai
    Liuhua Qiao Hospital, Guangzhou, Guangdong, China
    J Am Coll Cardiol 58:2118-27. 2011
    ..The purpose of this study was to assess the effect of scar tissue composition on engraftment of progenitor cells into infarcted myocardium...
  64. pmc Decorin modulates collagen matrix assembly and mineralization
    Yoshiyuki Mochida
    Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7455, United States
    Matrix Biol 28:44-52. 2009
    ..At the microscopic level, collagen fibers in S-DCN clones were thinner while those of AS-DCN clones were thicker and lacked directionality compared ..
  65. doi Corneal collagen cross-linking using riboflavin and ultraviolet-A irradiation: a review of clinical and experimental studies
    Maria Gkika
    Eye Institute of Thrace and Department of Ophthalmology, Medical School of Democritus University of Thrace, Dragana, 68100, Alexandroupolis, Greece
    Int Ophthalmol 31:309-19. 2011
    Corneal collagen cross-linking (CXL) using riboflavin and ultraviolet-A irradiation is a common method of tissue stabilization and has been developed primarily to address the need of treating keratoconus...
  66. doi Effect of superficial collagen patterns and fibrillation of femoral articular cartilage on knee joint mechanics-a 3D finite element analysis
    M E Mononen
    Department of Applied Physics, University of Eastern Finland, POB 1627, FI 70211 Kuopio, Finland
    J Biomech 45:579-87. 2012
    b>Collagen fibrils of articular cartilage have specific depth-dependent orientations and the fibrils bend in the cartilage surface to exhibit split-lines. Fibrillation of superficial collagen takes place in osteoarthritis...
  67. ncbi MT1-MMP: a potent modifier of pericellular microenvironment
    Yoshifumi Itoh
    Kennedy Institute of Rheumatology Division, Imperial College London, Hammersmith, London, United Kingdom
    J Cell Physiol 206:1-8. 2006
    ..Developing new means to inhibit "functional activity" of MT1-MMP may be a new direction to establish treatments for the diseases that MT1-MMP mediates such as cancer and rheumatoid arthritis...
  68. pmc Alternatively activated macrophages and collagen remodeling characterize the postpartum involuting mammary gland across species
    Jenean O'Brien
    Department of Medicine, Division of Medical Oncology, University of Colorado Denver, Mail Stop 8117, RC 1 S, 8401K, 12801 E 17 Avenue, UCD, Aurora, CO 80045, USA
    Am J Pathol 176:1241-55. 2010
    ..Fibrillar collagen levels and proteolysis increased dramatically during involution, and denatured collagen I acted as a strong ..
  69. pmc Interleukin 6 is required for the development of collagen-induced arthritis
    T Alonzi
    Istituto Ricerche di Biologia Molecolare P Angeletti, 00040 Pomezia, Rome, Italy
    J Exp Med 187:461-8. 1998
    ..In this study we have analysed the effect of ablating cytokine production in two mouse models of arthritis: collagen-induced arthritis (CIA) in DBA/1J mice and the inflammatory polyarthritis of tumor necrosis factor alpha (TNF-..
  70. ncbi Desmoplastic reaction in pancreatic cancer: role of pancreatic stellate cells
    M V Apte
    Pancreatic Research Group, Department of Gastroenterology, Bankstown Lidcombe and Liverpool Hospitals, NSW, Sydney, Australia
    Pancreas 29:179-87. 2004
  71. ncbi cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1)
    K Maeda
    Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan
    Biochem Biophys Res Commun 221:286-9. 1996
  72. ncbi Stress-strain measurements of human and porcine corneas after riboflavin-ultraviolet-A-induced cross-linking
    Gregor Wollensak
    Department of Ophthalmology, Technical University of Dresden, Dresden, Germany
    J Cataract Refract Surg 29:1780-5. 2003
    ..To evaluate the biomechanical effect of combined riboflavin-ultraviolet A (UVA) treatment on porcine and human corneas...
  73. doi Collagen crosslinking with riboflavin and ultraviolet-A light in keratoconus: long-term results
    Frederik Raiskup-Wolf
    Department of Ophthalmology, C G Carus University Hospital, Dresden, Germany
    J Cataract Refract Surg 34:796-801. 2008
    To prove the long-term dampening effect of riboflavin- and ultraviolet-A-induced collagen crosslinking on progressive keratoconus.
  74. pmc Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects
    Luis A Ortiz
    Division of Occupational Medicine, Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Proc Natl Acad Sci U S A 100:8407-11. 2003
    ..immediately after exposure to BLM also significantly reduced the degree of BLM-induced inflammation and collagen deposition within lung tissue...
  75. ncbi Attenuation of murine collagen-induced arthritis by a novel, potent, selective small molecule inhibitor of IkappaB Kinase 2, TPCA-1 (2-[(aminocarbonyl)amino]-5-(4-fluorophenyl)-3-thiophenecarboxamide), occurs via reduction of proinflammatory cytokines and
    Patricia L Podolin
    Respiratory and Inflammation Center of Excellence for Drug Discovery, GlaxoSmithKline, King of Prussia, PA 19406, USA
    J Pharmacol Exp Ther 312:373-81. 2005
    ..p., b.i.d., resulted in a dose-dependent reduction in the severity of murine collagen-induced arthritis (CIA)...
  76. doi Transepithelial corneal collagen cross-linking in keratoconus
    Antonio Leccisotti
    School of Biomedical Sciences, University of Ulster, Coleraine, UK
    J Refract Surg 26:942-8. 2010
    ..To evaluate the clinical effects of transepithelial corneal cross-linking (CXL) on keratoconic eyes pre-treated with substances enhancing epithelial permeability...
  77. ncbi Mechanism of stabilization of a bacterial collagen triple helix in the absence of hydroxyproline
    Angela Mohs
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 282:29757-65. 2007
    The Streptococcus pyogenes cell-surface protein Scl2 contains a globular N-terminal domain and a collagen-like domain, (Gly-Xaa-X'aa)(79), which forms a triple helix with a thermal stability close to that seen for mammalian collagens...
  78. ncbi A novel human serum lectin with collagen- and fibrinogen-like domains that functions as an opsonin
    M Matsushita
    Department of Biochemistry, Fukushima Medical College, Japan
    J Biol Chem 271:2448-54. 1996
    ..We report here a novel Ca(2+)-dependent and GlcNAc-binding lectin consisting of subunits of 35 kDa (P35) with a collagen-like sequence...
  79. ncbi Biomedical applications of collagen
    C H Lee
    Department of Pharmaceutics, College of Pharmacy, The University of Missouri Kansas City, 5005 Rockhill Rd, Katz Bdg 108, Kansas City, MO 64110, USA
    Int J Pharm 221:1-22. 2001
    b>Collagen is regarded as one of the most useful biomaterials...
  80. pmc Lifespan extension and delayed immune and collagen aging in mutant mice with defects in growth hormone production
    K Flurkey
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    Proc Natl Acad Sci U S A 98:6736-41. 2001
    ..Mutant dw(J)/dw animals show delays in age-dependent collagen cross-linking and in six age-sensitive indices of immune system status...
  81. ncbi Increased resistance of crosslinked cornea against enzymatic digestion
    Eberhard Spoerl
    Department of Ophthalmology, Universitatsklinikum CGC, Fetscherstrasse 74, D 01307 Dresden, Germany
    Curr Eye Res 29:35-40. 2004
    b>Collagen-crosslinking using combined riboflavin/ UVA treatment has been developed by us as a new treatment for keratoconus by stiffening the collagenous matrix...
  82. ncbi Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling
    S E Bell
    Department of Pathology and Laboratory Medicine, Texas A and M University System Health Science Center, College Station 77843 1114, USA
    J Cell Sci 114:2755-73. 2001
    ..A recombinant portion of CMG-2 was found to bind collagen type IV and laminin, suggesting a potential role in basement membrane matrix synthesis and assembly...
  83. ncbi Collagen scaffolds for tissue engineering
    Julie Glowacki
    Department of Orthopedic Surgery, Brigham and Women s Hospital, Boston, MA, USA
    Biopolymers 89:338-44. 2008
    ..Of the many scaffold materials being investigated, collagen type I, with selective removal of its telopeptides, has been shown to have many advantageous features for both of ..
  84. ncbi Pancreatic carcinoma cells induce fibrosis by stimulating proliferation and matrix synthesis of stellate cells
    Max G Bachem
    Department of Clinical Chemistry, University of Ulm, D 89070 Ulm, Germany
    Gastroenterology 128:907-21. 2005
    ..The aims of this study were to examine the cellular and molecular mechanisms of fibrogenesis associated with pancreatic adenocarcinomas...
  85. doi Regulation of TGFbeta1-mediated collagen formation by LOX-1: studies based on forced overexpression of TGFbeta1 in wild-type and lox-1 knock-out mouse cardiac fibroblasts
    Changping Hu
    Cardiovascular Medicine, University of Arkansas for Medical Sciences and Central Arkansas Veterans Healthcare System, Little Rock, Arkansas 72205 7199, USA
    J Biol Chem 283:10226-31. 2008
    ..on the role of LOX-1, a lectin-like 52-kDa receptor for oxidized low density lipoprotein, in TGFbeta(1)-mediated collagen expression and underlying signaling in mouse cardiac fibroblasts...
  86. doi Cytoplasmic superoxide causes bone fragility owing to low-turnover osteoporosis and impaired collagen cross-linking
    Hidetoshi Nojiri
    Molecular Gerontology, Tokyo Metropolitan Institute of Gerontology, Itabashi ku, Tokyo, Japan
    J Bone Miner Res 26:2682-94. 2011 cytoplasmic superoxide caused a distinct weakness in bone stiffness and decreased BMD, aging-like changes in collagen cross-linking, and transcriptional alterations in the genes associated with osteogenesis...
  87. pmc Inhibition of signal transducer and activator of transcription 3 (STAT3) attenuates interleukin-6 (IL-6)-induced collagen synthesis and resultant hypertrophy in rat heart
    Saiful Anam Mir
    Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700 019, India
    J Biol Chem 287:2666-77. 2012
    IL-6 has been shown to play a major role in collagen up-regulation process during cardiac hypertrophy, although the precise mechanism is still not known...
  88. doi Collagen cross-linking: a new treatment paradigm in corneal disease - a review
    Grant R Snibson
    Centre for Eye Research Australia, Department of Ophthalmology, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia
    Clin Experiment Ophthalmol 38:141-53. 2010
    The last 2 years has seen a marked increase in the prominence of corneal collagen cross-linking as a treatment strategy for progressive keratoconus...
  89. doi The EBV oncogene LMP1 protects lymphoma cells from cell death through the collagen-mediated activation of DDR1
    Fathima Zumla Cader
    School of Cancer Sciences, University of Birmingham, Birmingham, United Kingdom and
    Blood 122:4237-45. 2013
    ..a tumor microenvironment that is composed of a variety of cell types, as well as noncellular components such as collagen. Although HRS cells harbor oncogenic Epstein-Barr virus (EBV) in approximately 50% of cases, it is not known if ..
  90. doi The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves
    Ahmet Bozkurt
    Department of Plastic Surgery, Reconstructive and Hand Surgery, Burn Center, Medical Faculty, RWTH Aachen University, Aachen, Germany
    Biomaterials 33:1363-75. 2012
    ..In the present investigation, we present a collagen-based micro-structured nerve guide (Perimaix) for the repair of 2 cm rat sciatic nerve defects...
  91. doi miR-133a mediates TGF-β-dependent derepression of collagen synthesis in hepatic stellate cells during liver fibrosis
    Christoph Roderburg
    Department of Medicine III, University of Aachen RWTH, 52074 Aachen, Germany
    J Hepatol 58:736-42. 2013
    ..miRNAs are novel regulators of organ fibrosis. miR-133a plays a role in cardiac and muscle remodeling, but its function in the liver is unclear. We therefore aimed at evaluating a possible function of miR-133a in hepatofibrogenesis...
  92. pmc Structure of the discoidin domain receptor 1 extracellular region bound to an inhibitory Fab fragment reveals features important for signaling
    Federico Carafoli
    Department of Life Sciences, Imperial College London, London SW7 2AZ, UK
    Structure 20:688-97. 2012
    ..DDR1 and DDR2, are constitutively dimeric receptor tyrosine kinases that are activated by triple-helical collagen. Aberrant DDR signaling contributes to several human pathologies, including many cancers...
  93. ncbi Collagen in the scarless fetal skin wound: detection with picrosirius-polarization
    Leila Cuttle
    Department of Paediatrics and Child Health, University of Queensland, Royal Children s Hospital, Queensland, Australia
    Wound Repair Regen 13:198-204. 2005
    ..The comparison of collagen structure between these two different mechanisms of healing may elucidate the process of scarless wound healing...
  94. ncbi Safety of UVA-riboflavin cross-linking of the cornea
    Eberhard Spoerl
    Department of Ophthalmology, Universitatsklinikum Dresden, Dresden, Germany
    Cornea 26:385-9. 2007
    To study potential damage to ocular tissue during corneal collagen cross-linking (X-linking) by means of the riboflavin/UVA (370 nm) approach.
  95. pmc Nature designs tough collagen: explaining the nanostructure of collagen fibrils
    Markus J Buehler
    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 1 272, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 103:12285-90. 2006
    b>Collagen is a protein material with superior mechanical properties. It consists of collagen fibrils composed of a staggered array of ultra-long tropocollagen (TC) molecules...
  96. doi In situ multi-level analysis of viscoelastic deformation mechanisms in tendon collagen
    H S Gupta
    School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    J Struct Biol 169:183-91. 2010 the fibril and fibre levels has been developed, capturing this behaviour by serially coupling a Voigt element (collagen fibril), with two Maxwell elements (non-collagenous matrix between fibrils and fibres)...
  97. ncbi Matrix production and collagen structure are enhanced in two types of osteogenic progenitor cells by a simple fluid shear stress stimulus
    R M Delaine-Smith
    Kroto Research Institute, Department of Materials Science and Engineering, University of Sheffield, Sheffield, UK
    Eur Cell Mater 24:162-74. 2012
    ..Both types of progenitor cell subjected to FSS showed increases in collagen secretion and apparent collagen organisation as imaged by second harmonic generation.
  98. pmc Bone regeneration with low dose BMP-2 amplified by biomimetic supramolecular nanofibers within collagen scaffolds
    Sungsoo S Lee
    Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA
    Biomaterials 34:452-9. 2013
    ..biological role of syndecan and fibronectin, were allowed to form gel networks within the pores of an absorbable collagen scaffold by simply infiltrating dilute solutions of the peptide amphiphile, heparan sulfate, and BMP-2...
  99. ncbi The discoidin domain receptor tyrosine kinases are activated by collagen
    W Vogel
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Mol Cell 1:13-23. 1997
    ..Here, we show that the mammalian DDR receptors bind and are activated by specific types of collagen. Stimulation of DDR receptor tyrosine kinase activity requires the native triple-helical structure of collagen ..
  100. pmc Discoidin domain receptor tyrosine kinases: new players in cancer progression
    Rajeshwari R Valiathan
    Department of Pathology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cancer Metastasis Rev 31:295-321. 2012
    ..DDRs) play a key role in cancer progression by regulating the interactions of tumor cells with their surrounding collagen matrix. The DDRs are the only RTKs that specifically bind to and are activated by collagen...
  101. doi Collagen-related genes influence the glaucoma risk factor, central corneal thickness
    Eranga N Vithana
    Singapore Eye Research Institute, Singapore
    Hum Mol Genet 20:649-58. 2011
    ..5 × 10⁻⁹). These findings implicate the involvement of collagen genes influencing CCT and thus, possibly the pathogenesis of glaucoma.

Research Grants82

  1. UV-induced collagen reduction: treating skin scleroderma
    Sewon Kang; Fiscal Year: 2005
    ..The exact causes of scleroderma are unknown, however, the hallmark of the disease process is over-production of collagen. Currently, there is no safe and effective therapy for the disease...
  2. Serpin Modulation of Inflammatory Vasculitis; Potential for Immunomodulatory Ther
    ALEXANDRA ROSE LUCAS; Fiscal Year: 2010
    ..with proven anti-inflammatory actions in atherosclerosis (Fig/s 2 &3), transplant vasculopathy, and collagen-induced arthritis in animal models...
  3. Development of a Nonpsychotropic Cannabinoid Agonist for the Treatment of Sclerod
    Robert B Zurier; Fiscal Year: 2012
    ..Fibrosis, the characteristic histopathology in patients with SSc, includes increased production of collagen and other extracellular matrix (ECM) components, and accumulation of alpha smooth muscle actin (ASMA) positive ..
  4. Roles of Nitric Oxide and Superoxide in Cystitis
    ANTHONY JOHN KANAI; Fiscal Year: 2013
    ..A chronic phase can develop in 6-12 months with vascular endothelial cell damage, ischemia, collagen deposition and decreased bladder compliance...
  5. Lung Lysyl Oxidase Regulation by Metal Ion Homeostasis
    Wande Li; Fiscal Year: 2013
    ..Lysyl oxidase (LO), a copper-(Cu) dependent enzyme, oxidizes peptidyl lysine residues in substrates, e.g., collagen, elastin and histone H1, essential for organization and stabilization of the extracellular matrix (ECM) and the ..
  6. Comparative Resistance Exercise Effects on Knee Osteoarthritis Pain, Functional I
    KEVIN ROBERT VINCENT; Fiscal Year: 2013
    ..cartilage biomarker turnover than CRX or CON by month four (using C-propeptide of type II procollagen [CTX-II], Collagen Type II C- telopeptide [CPII])...
    Chella David; Fiscal Year: 2009
    ..b>Collagen-induced arthritis (CIA) susceptible HLA transgenic mice produce rheumatoid factor and anti-cyclic citrullinated ..
  8. Collagen X and heparan sulfate proteoglycan in the hematopoietic stem cell niche.
    ELIZABETH G SWEENEY; Fiscal Year: 2012
    ..During my post doctoral studies, I have been able to further characterize the lymphopoietic defects in the collagen X transgenic and null mice, as well as describe the immune response defects at both a cellular level in vitro and ..
  9. Biology and Pathologies of Type V Collagen
    Daniel S Greenspan; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Proposed are studies into the nature of collagen V [col(V)] and its roles in human disease: 1) Mice null for the Col5a3 gene, encoding the poorly characterized 13(V) chain, have been produced in the ..
  10. Chronic Sleep Restriction Increases Immunity to Autoantigen: Role of the SNS
    Arnold E Postlethwaite; Fiscal Year: 2010
    ..chronic disrupted sleep, we have been studying the effect of chronic (14-30 day) sleep restriction on type II collagen (CII)-induced arthritis (CIA) in DBA/1LacJ mice...
  11. Alternative Macrophage Activation Limits Immunopathology
    DEBROSKI R HERBERT; Fiscal Year: 2011
    ..We now have evidence that arginase itself is responsible for blocking IL-12/23p40 induction, driving collagen production, and limiting severe inflammation of liver and intestine...
  12. Immune Mechanism of Rejection in Human Lung Allografts
    Thalachallour Mohanakumar; Fiscal Year: 2013
    ..of recent LTx recipients (142 from our center) has demonstrated that 30% have pre-existing Abs to self-Ags (collagen V (ColV) and K-alpha 1 Tubulin (K?1T)) and these patients demonstrate an increased risk of developing PGD (odds ..
  13. Role of Peroxidasin in Glomerular Matrix Homeostasis
    Gautam Bhave; Fiscal Year: 2013
    ..Peroxidasin uses HOX to form novel sulfilimine bonds (S=N) in basement membrane collagen IV, the first identified function for peroxidasin...
  14. BBP (Bone Morphogenetic Protein Binding Peptide) and Bone Healing
    SAMUEL SCOTT MURRAY; Fiscal Year: 2013
    ..intramembranous bone formation and chondrogenesis by quantitative histology and immunohistology of types I and II collagen and osteocalcin...
  15. A Bifunctional Katp Channel Activator and Redox Mimetic for BPD
    Kanneganti Murthy; Fiscal Year: 2013
    ..peroxynitrite formation (3-nitrotyrosine), poly(ADP-ribose) formation, fibrosis (Mason Trichrome staining for collagen), R- 801 levels, and the protein expression of pro-inflammatory genes, including eotaxin, G-CSF, GM-CSF, IFN-?, ..
    Tibor T Glant; Fiscal Year: 2013
    ..Many of these quantitative trait loci (QTLs) correlated with those identified in collagen-induced arthritis (CIA) in mice (mCia) and rats, colocalized with QTLs of other autoimmune models, and ..
  17. Runx Proteins and Sex Steroid Signaling in Bone
    Baruch Frenkel; Fiscal Year: 2009
    ..These inhibitory activities are important in light of recent data from [unreadable]1(I)collagen-Runx2 transgenic mice, indicating that Runx2 must be restrained in order to keep bone turnover in check and ..
  18. A Novel Thioredoxin Mimetic Prodrug for Prevention of Bronchopulmonary Dysplasia
    Prakash Jagtap; Fiscal Year: 2013
    ..peroxynitrite formation (3-nitrotyrosine), poly(ADP-ribose) formation, fibrosis (Mason Trichrome staining for collagen), R-908 and R-901 levels, and pro-inflammatory gene expression...
  19. Roles of oxidation and inflammation in aortic stiffening
    David G Harrison; Fiscal Year: 2013
    ..Biochemical and experimental studies have also implicated reactive oxygen species in modifications of elastin and collagen that would promote arterial stiffening...
  20. Modeling TH17 Cells in Acute GVHD
    JONATHAN STUART SERODY; Fiscal Year: 2010 the pathogenesis of multiple different autoimmune diseases including experimental autoimmune encephalitis, collagen-induced arthritis, and inflammatory bowel disease...
  21. High throughput screen for selective inhibitors of cyclophilin B
    Richard J Bram; Fiscal Year: 2013
    ..we found that mice completely lacking CypB have normal development and fertility with a minor phenotype involving collagen processing, demonstrating that loss of CypB is not required for viability of the organism...
  22. Novel Collagen II Alternative Transcripts and Mouse Skeletal Development
    Audrey McAlinden; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: This grant proposal will investigate the significance of novel type II collagen (Col2a1 gene) alternative transcripts within the context of skeletal development, primarily by generation of a ..
  23. IGFBP-5 and Hsp70 in Extracellular Matrix Homeostasis and Fibrosis
    Kristen L Veraldi; Fiscal Year: 2013
    ..IGFBP-5 induces expression of the major extracellular matrix proteins fibronectin and collagen I...
  24. Salivary-based Bone-loss Marker Detection Platform for Point-of-Care Screening
    MANAL BESHAY; Fiscal Year: 2012
    ..assess two key bone activities: bone formation, presented in osteocalcin (OC), and bone resorption, presented in collagen crosslink deoxypyridinoline (D-PYR)...
  25. Asthma Increases Vaso-occlusion in Sickle Cell Disease
    Kirkwood A Pritchard; Fiscal Year: 2013
    ..experimental asthma induced by ovalbumin (OVA) sensitization of SCD mice increases pulmonary inflammation and collagen deposition in SCD mice to a much greater extent than in OVA-sensitized control mice...
  26. Fate of Lung Stem Cells During Pulmonary Disease
    Susan M Majka; Fiscal Year: 2012
    ..Myofibroblasts were characterized by collagen/elastin synthesis...
  27. Hematopoietic Stem Cell Transplantation in Osteogenesis Imperfecta
    Meenal Mehrotra; Fiscal Year: 2012
    ..OI or "brittle bone disease", a genetic disorder resulting from the abnormal amount and/or structure of Type I collagen, is the most common hereditary bone disease...
  28. Anti-Fibrotic Therapy for Scleroderma/SSc
    Latha Paka; Fiscal Year: 2010
    ..caused by a surplus of extracellular matrix protein localized in the skin, or it may become systemic with excess collagen collecting in the kidneys, lungs, gastrointestinal tract, and heart...
  29. Role of Matrix Metalloproteinase-1 in Endothelial Cell Senescence
    Catherine D Mao; Fiscal Year: 2010
    ..MMP-1, in addition to degrade collagen, can signals via cleavage and activation of the proteinase-activated-receptor (PAR)-1...
  30. Chemokine Decoy Receptor for Therapy of Autoimmune Arthritis
    Kanneganti Murthy; Fiscal Year: 2012
    ..Male DBA mice inoculated with collagen in CFA and rechallenged on Day 21 to induce autoimmune arthritis, will be treated with mR-421 (2, 4, 8 mg/kg), an ..
  31. Regulation of vasculature by estrogen receptors during aging in mouse models
    Margaret Markiewicz; Fiscal Year: 2013
    ..Furthermore, we observed increased collagen synthesis in the ER ?-/- mice, while a decrease was observed in ER?? -/- mice...
  32. Conditional Knockout of Calcium Receptors in Bone Cells
    Dolores M Shoback; Fiscal Year: 2013
    ..and osteoblast- specific Tg mice expressing Cre recombinase under the control of specific bone promoters (type 1 collagen, osteocalcin, osterix, dentin matrix protein 1) to develop conditional knockouts of the CaR targeted to bone cell ..
  33. Mechanisms of Airway Smooth Muscle Hypertrophy
    Marc B Hershenson; Fiscal Year: 2013
    ..In the "new BPD," alveolar septa are thickened with collagen and 1-smooth muscle actin-positive, transforming growth factor (TGF)-[unreadable]-positive myofibroblasts...
  34. Adult and Transgenerational Toxicity Due to Developmental TCDD Exposure
    Tracie R Baker; Fiscal Year: 2013
    ..I will identify the source of these changes in skeletal development examining bone and collagen by staining and measuring alterations in gene expression...
    Veena Taneja; Fiscal Year: 2013
    ..b>Collagen-induced arthritis (CIA) susceptible HLA transgenic mice produce rheumatoid factor and anti-cyclic citrullinated ..
  36. Aligned Tendon or Ligament Derived Matrix Nanoscaffolds for Rotator Cuff Repair
    Dianne Little; Fiscal Year: 2013
    ..and none mimic the interdigitating multi-layered structure of the normal rotator cuff which has layers of collagen aligned at 45 degrees to each other to allow efficient load transfer and distribution, suture retention and ..
  37. Aldosterone, Autophagy, and Arterial Stiffness
    Jane A Leopold; Fiscal Year: 2013
    ..of the vessel media characterized by vascular smooth muscle cell disarray, increased deposition of aberrant collagen and fragmentation of elastin, all of which contribute to decrease a functional decrease in vascular compliance...
  38. Pluripotent human stem cells as models for normal and diseased trophoblast
    ROBERT MICHAEL ROBERTS; Fiscal Year: 2013
    ..of the EVT derived from BMP-hESC to invade through cross-linked, relatively stiff matrices, such as Type I collagen, and to interact with microcapillaries cultured within such matrices...
  39. TNF Family Members and Control of Fibrotic Disease
    Michael Croft; Fiscal Year: 2013
    ..applicant): Human systemic sclerosis (SSc) is an autoimmune-like disease that results in excessive production of collagen in the skin as well as tissues such as kidney, heart, and lung...
  40. Nonenzymatic glycation, bone quality, and microdamage in type 2 diabetic bone
    Eve Donnelly; Fiscal Year: 2013
    ..The immediate goal of the proposed work is to provide critical training in collagen biochemistry and bone micro damage to enable characterization of the role of nonenzymatic glycation in bone ..
  41. Evaluating Novel Multi-kinase Peptide Inhibitors as a Treatment for Rheumatoid Ar
    DAVID MEN HWEI TSAI; Fiscal Year: 2013
    ..effects of AX-3 and its active component on joint degradation and various biomarkers will be assessed using a collagen- induced RA rat model...
  42. Regulation of Chronic Colitis by Mesenchymal Stem Cells
    Matthew B Grisham; Fiscal Year: 2013
    ..they suppress the inflammation observed in animal models of autoimmune encephalomyelitis, allograft rejection, collagen-induced arthritis and graft vs. host disease...
  43. Impact of smoking on immune response and arthritis in humanized mice
    Veena Taneja; Fiscal Year: 2011
    ..b>Collagen-induced arthritis (CIA) susceptible HLA transgenic mice produce rheumatoid factor and anti-cyclic citrullinated ..
  44. Role of SIRT3 in fetal programming and kidney dysfunction
    Karen R Jonscher; Fiscal Year: 2013
    ..Dr. Jonscher's compelling and novel preliminary data show altered lipid accumulation, collagen deposition and protein hyperacetylation in kidneys from juvenile offspring exposed to maternal obesity, ..
  45. Biochemical, Biomechanical & Morphological Properties of Quantitative Ultrasound
    Barbara L McFarlin; Fiscal Year: 2010
    ..frequency-dependent QUS-derived parameters in the rat cervix as ripening occurs, with cervix tissue biochemical (collagen and water), biostructural (birefringence, histology, 3-D impedance map) biomechanical (stress-strain) properties...
  46. The Role of Carboxypeptidase B and its Substrates in Rheumatoid Arthritis
    William H Robinson; Fiscal Year: 2013
    ..developed dramatically more severe arthritis as compared to wild type (WT) controls following transfer of anti-collagen antibodies...
  47. Caveolins in the Pathogenesis of Idiopathic Pulmonary Fibrosis
    Dan N Predescu; Fiscal Year: 2010 cellular proliferation (especially the fibroblast) and accumulation of extracellular material (mostly collagen like), that finally leads to irreversible destruction of lung architecture...
  48. Mechanisms in transcriptional regulation during cardiac hypertrophy
    Maha Abdellatif; Fiscal Year: 2013
    ..g. ANF, BNP, alpha skeletal actin, collagen, etc...
  49. Multiplexed DNA Origami-Based Biomarker Detection Assay for Early Dx of Arthritis
    John E Mueller; Fiscal Year: 2013
    ..AR is associated with the breakdown of collagen and the production of inflammation-related factors such as cytokines...
  50. Thrombospondin 4 A1
    JOHN W LAWLER; Fiscal Year: 2010
    ..MIDAS) and the adjacent to MIDAS motif will be mutated to alanines to establish the importance of these sites in collagen and zinc binding, and matrix assembly...
  51. Periostin Regulation of Lung Fibrosis
    Bethany B Moore; Fiscal Year: 2013
    ..We recently demonstrated that periostin can induce mesenchymal cell proliferation, collagen expression and ability to close a scratch wound...
  52. Regulation of Lung Fibrosis by Alternatively Activated Macrophages and Arginase-1
    ROBERT MATTHEW TIGHE; Fiscal Year: 2013
    ..Polyamines are involved in cell proliferation while proline is a direct precursor of collagen. The role of arginase-1 expression by macrophages and fibroblasts in progressive fibrosis has not been explored...
    Namasivayam Ambalavanan; Fiscal Year: 2012
    ..novel observations that Thy-1 null mice have IAD and increased phospho-Smad2, (-smooth muscle actin, interstitial collagen, tissue lung resistance (but normal airway resistance), and decreased lung compliance, demonstrating that absence ..
  54. The Role of FKBP10 in Recessive Osteogenesis Imperfecta
    Caressa Lietman; Fiscal Year: 2013
    ..complexes with Heat Shock Protein 47 (HSP47) and together they are thought to be involved in chaperoning of collagen in the endoplasmic reticulum [4, 5]...
  55. Matrix Otopathology
    MICHAEL ANNE NMI GRATTON; Fiscal Year: 2011
    ..One such matrix, the basement membrane, utilizes type IV collagen to form a unique protein lattice to which other matrix proteins bind...
  56. A Novel Model of, and Pre-clinical Therapy for, Scleroderma Lung Disease
    Sergei P Atamas; Fiscal Year: 2012
    ..features of human SLD, causing infiltration of lymphocytes, disturbances in cytokine milieu, and a tendency to collagen accumulation...
  57. Homocysteine and Angiotensin II in Renovascular Remodeling
    Utpal Sen; Fiscal Year: 2013
    ..To determine whether the Hcy instigates Ang II hypertension and renovascular fibrosis, in part, by inducing collagen IV, MMP-2, -9, -13;TIMP-1,-2, -3, -4;and if FA mitigates this renovascular fibrosis;and (3) To determine whether ..
  58. Thiol Isomerases During Thrombus Formation
    Barbara C Furie; Fiscal Year: 2013
    ..on/off rates, platelet thrombus embolization, microparticle accumulation, vWF accumulation, TF expression, and collagen exposure will be evaluated in the laser-induced vessel wall injury model and the ferric chloride model in a ..
  59. Genetically testing interactions of ER and oxidative stresses in retinal disease
    Douglas Gould; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Mutation of the type IV collagen alpha 1 gene (Col4a1) gene cause retinal phenotypes in mice that resemble age-related macular degeneration (AMD)...
  60. Identification of Mechanoresponsive Promoter Elements in Chondrogenesis
    Dominik R Haudenschild; Fiscal Year: 2013
    ..of mechanotransduction pathways in cartilage include extracellular matrix genes such as Aggrecan and type II collagen and Cartilage Oligomeric Matrix Protein (COMP)...
  61. Experimental engineering of ovarian grafting to promote angiogenesis for fertilit
    GLENN LEWIS SCHATTMAN; Fiscal Year: 2010
    ..In the second, we will reduce collagen bulk in human ovarian tissue to test whether the higher fiber density is a barrier to vascular invasion and ..
  62. 3D Skin Model to Test Toxic and Sensitizing Potentials of Environmental Chemicals
    Akira Takashima; Fiscal Year: 2010
    ..Under Aim 2, we will reconstitute a 3D skin model by: a) culturing the NS FB line in hydrated collagen gel to form a dermal-equivalent matrix, b) culturing a KC biosensor clone on the top of the collagen matrix, and ..
  63. Humanized Transgenic Mice Reproduce the Disease Evolution in Severe RA
    Grete Sonderstrup; Fiscal Year: 2010
    ..In the recently reported D1CC transgenic mice that express the class II transactivator (CIITA) under the rat collagen II promoter, we presented an inducible disease syndrome, which very closely reproduces the late stages of RA with ..
    Harvey Lodish; Fiscal Year: 2007
    ..during adipocyte differentiation), and secreted proteins not stimulated by insulin like adipocyte-specific (3 VI collagen (which, unlike ubiquitously expressed type VI collagen has a 100 AA insert in the C3 domain identified by the PI ..
    ELLEN ZIMMERMANN; Fiscal Year: 1999
    ..IGF-I is a potent mitogen for fibroblasts and smooth muscle cells' and stimulates synthesis of several collagen types in vitro...
    James McCarthy; Fiscal Year: 1999
    ..well established role for laminin in promoting tumor cell adhesion, there is also an important role for type IV collagen in mediating the adhesion, migration and invasion of transformed and malignant tumors...
    Richard Mayne; Fiscal Year: 2002
    Experiments will investigate the structure and formation of the collagen fibrils of hyaline cartilage from mammalian and avian species...
    SHERRILL ADAMS; Fiscal Year: 1993
    Type III collagen is found in most connective tissues, but is not found in hyaline cartilage. However, a unique, previously undescribed RNA species has been detected in chick cartilage...
  69. Collagenase degradation in extracellular matrix in aging
    Gary J Fisher; Fiscal Year: 2010
    ..Reduced function of aged skin is largely caused by irreversible destruction of fibrillar collagen, the major structural protein in skin connective tissue (dermis)...
  70. Expressions of Specialized Collagens in the Cornea
    Marion K Gordon; Fiscal Year: 2010
    ..b>Collagen fibrils in the corneal stroma are thin, uniform diameter structures, evenly spaced apart at discreet distances, ..
    Richard Mayne; Fiscal Year: 1992
    The structure and function of the minor cartilage collagens called type IX collagen and 1 Alpha, 2 Alpha, 3 Alpha collagen will be investigated...
    Subha Sen; Fiscal Year: 2002
    ..Excess collagen accumulations are known to increase the heart's rigidity, compromising its function and leading to failure...
    MARK ZORN; Fiscal Year: 1991
    ..The sclera is made largely of collagen types I and III. Collagen type III is less able to resist stretch than is collagen type I...
    Richard Berg; Fiscal Year: 1992
    The regulation of collagen has clear health-related implications because a number of human diseases or dysfunctions such as rheumatoid arthritis, pulmonary fibrosis, cirrhosis, atherosclerosis, acute and chronic inflammation, Ehler's ..
    Jerome F Strauss; Fiscal Year: 2010
    ..We now propose to 1) To determine whether variants in genes encoding proteins involved in collagen synthesis and posttranslational modification, including the SERPINH1 gene, which encodes a chaperone that ..
    Deborah Redford Badwal; Fiscal Year: 1993
    ..increased our understanding of the molecular basis of heritable connective tissue disorders affecting type I collagen. The molecular defects range from a single base changes and multi-exon deletions to abnormalities of RNA splicing...
    Mitsuo Yamauchi; Fiscal Year: 1991
    ..bone disorders which express themselves as a loss in bone mass (osteoporosis), undermineralization of collagen (osteomalacia) and failure of collagen to mineralize (rachitic bone)...
  78. Studies of Collagen Gene Regulation in Two Murine Models
    Stephen Clark; Fiscal Year: 2005
    ..Both mutations display excessive accumulation of collagen and other extracellular matrix components in the skin, a hallmark feature of the human disease...
  79. The Role of P4H in Tissue-Titanium Integration
    Takahiro Ogawa; Fiscal Year: 2006
    ..P4H functions during post-translational hydroxylation of collagen chains, a process essential for cross-linking individual collagen molecules into a trimetric mature collagen to ..
  80. Investigating Ultrastructural Collagen Changes in Osteogenesis Imperfecta
    Joseph Wallace; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): The lack of understanding of how altered collagen structure in Osteogenesis Imperfecta (Ol) translates to insufficiencies in tissue integrity is a critical impediment to the implementation of ..
  81. Enabling ACL Repair Using Proprietary Collagen Device
    Emma Wright; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): A collagen-platelet composite has been developed and validated by the investigators in a canine model to heal partial ACL tears both biomechanically and histologically...
    DANIEL GREENSPAN; Fiscal Year: 2000
    DESCRIPTION: This proposal comprises studies of type V collagen and its roles in human diseases and studies into the tissue-specific expression of the type VII collagen gene...