phosphotransferases

Summary

Summary: A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.

Top Publications

  1. ncbi Two-component signal transduction
    A M Stock
    Center for Advanced Biotechnology and Medicine and Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Annu Rev Biochem 69:183-215. 2000
  2. pmc Distinct modes of ATR activation after replication stress and DNA double-strand breaks in Caenorhabditis elegans
    Tatiana Garcia-Muse
    DNA Damage Response Laboratory, Cancer Research UK, The London Research Institute, Clare Hall Laboratories, South Mimms, UK
    EMBO J 24:4345-55. 2005
  3. pmc Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli
    Kazushi Suzuki
    Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322, USA
    J Bacteriol 184:5130-40. 2002
  4. ncbi Compilation of all genes encoding two-component phosphotransfer signal transducers in the genome of Escherichia coli
    T Mizuno
    Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan
    DNA Res 4:161-8. 1997
  5. ncbi Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli
    A K Pernestig
    Microbiology and Tumorbiology Center, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 276:225-31. 2001
  6. ncbi The spindle-assembly checkpoint in space and time
    Andrea Musacchio
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Rev Mol Cell Biol 8:379-93. 2007
  7. pmc The Arabidopsis thaliana response regulator ARR22 is a putative AHP phospho-histidine phosphatase expressed in the chalaza of developing seeds
    Jakub Horak
    Zentrum für Molekularbiologie der Pflanzen Pflanzenphysiologie, Universitat Tubingen, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    BMC Plant Biol 8:77. 2008
  8. pmc An asymmetry-to-symmetry switch in signal transmission by the histidine kinase receptor for TMAO
    Jason O Moore
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Structure 20:729-41. 2012
  9. pmc Integral membrane lipid phosphatases/phosphotransferases: common structure and diverse functions
    Yury J Sigal
    Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7090, USA
    Biochem J 387:281-93. 2005
  10. pmc Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold
    J E Walker
    EMBO J 1:945-51. 1982

Research Grants

  1. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
  2. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
  3. Lauren Endres; Fiscal Year: 2014
  4. Xenoestrogen regulation of Leydig cells
    BENSON TOKUNBO AKINGBEMI; Fiscal Year: 2010
  5. Pyruvate Dehydrogenase kinase as Therapeutic Target for Hepatic Steatosis, Obesit
    Robert A Harris; Fiscal Year: 2012
  6. SHAOCHUNG VICTOR HSIA; Fiscal Year: 2016
  7. Yun Fai Chris Lau; Fiscal Year: 2014
  8. Cory Teuscher; Fiscal Year: 2014
  9. Role of Chromogranin A in Metabolic Syndrome
    Sushil K Mahata; Fiscal Year: 2013
  10. VINCENT SCOTT TAGLIABRACCI; Fiscal Year: 2014

Detail Information

Publications329 found, 100 shown here

  1. ncbi Two-component signal transduction
    A M Stock
    Center for Advanced Biotechnology and Medicine and Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Annu Rev Biochem 69:183-215. 2000
    ..Thus detailed analyses of a relatively small number of representative proteins provide a foundation for understanding this large family of signaling proteins...
  2. pmc Distinct modes of ATR activation after replication stress and DNA double-strand breaks in Caenorhabditis elegans
    Tatiana Garcia-Muse
    DNA Damage Response Laboratory, Cancer Research UK, The London Research Institute, Clare Hall Laboratories, South Mimms, UK
    EMBO J 24:4345-55. 2005
    ..We propose that atl-1 and rad-5/clk-2 respond to single-stranded DNA generated by replication stress and function with atm-1 following DSB resection...
  3. pmc Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli
    Kazushi Suzuki
    Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322, USA
    J Bacteriol 184:5130-40. 2002
    ..Collectively, these results demonstrate that uvrY resides downstream from csrA in a signaling pathway for csrB and that CsrA stimulates UvrY-dependent activation of csrB expression by BarA-dependent and -independent mechanisms...
  4. ncbi Compilation of all genes encoding two-component phosphotransfer signal transducers in the genome of Escherichia coli
    T Mizuno
    Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan
    DNA Res 4:161-8. 1997
    ..In addition, E. coli has five hybrid sensory kinases. The precise location of each ORF was mapped on a physical map of the entire E. coli genome. All of these ORFs were then compiled and annotated extensively...
  5. ncbi Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli
    A K Pernestig
    Microbiology and Tumorbiology Center, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 276:225-31. 2001
    ..These results provide the first biochemical evidence as well as genetic support for a link between BarA and UvrY, suggesting that the two proteins constitute a new two-component system for gene regulation in Escherichia coli...
  6. ncbi The spindle-assembly checkpoint in space and time
    Andrea Musacchio
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Rev Mol Cell Biol 8:379-93. 2007
    ..These studies are finally starting to reveal the mechanisms of checkpoint activation and silencing during mitotic progression...
  7. pmc The Arabidopsis thaliana response regulator ARR22 is a putative AHP phospho-histidine phosphatase expressed in the chalaza of developing seeds
    Jakub Horak
    Zentrum für Molekularbiologie der Pflanzen Pflanzenphysiologie, Universitat Tubingen, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    BMC Plant Biol 8:77. 2008
    ..While the functions of the other TCS elements in Arabidopsis, such as histidine kinases (AHKs), histidine-containing phosphotransfer proteins (AHPs) and A-type and B-type ARRs are becoming evident, the role of ARR22 is poorly understood...
  8. pmc An asymmetry-to-symmetry switch in signal transmission by the histidine kinase receptor for TMAO
    Jason O Moore
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Structure 20:729-41. 2012
    ..TorS-TorT(apo) is an asymmetric 2:2 complex that binds TMAO with negative cooperativity to form a symmetric active kinase...
  9. pmc Integral membrane lipid phosphatases/phosphotransferases: common structure and diverse functions
    Yury J Sigal
    Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7090, USA
    Biochem J 387:281-93. 2005
    ..These five groups of homologous proteins, which we collectively term LPTs (lipid phosphatases/phosphotransferases), are characterized by a core domain containing six transmembrane-spanning alpha-helices connected by ..
  10. pmc Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold
    J E Walker
    EMBO J 1:945-51. 1982
    ....
  11. doi Ethylene signaling: identification of a putative ETR1-AHP1 phosphorelay complex by fluorescence spectroscopy
    Benjamin Scharein
    Biochemie der Pflanzen, Heinrich Heine Universitat, Universitatsstr 1, 40225 Dusseldorf, Germany
    Anal Biochem 377:72-6. 2008
    ..Thereby it provides the basics for a more quantitative elucidation of phosphorelay modules acquired in phytohormone signaling...
  12. ncbi Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein
    Tea Vallenius
    Haartman Institute and Helsinki University Central Hospital, Biomedicum Helsinki, P O Box 63, 00014 University of Helsinki, Finland
    J Cell Sci 115:2067-73. 2002
    ..Taken together these results establish the CLP-36 PDZ-LIM protein as an adapter, recruiting the Clik1 kinase to actin stress fibers in nonmuscle cells, and suggest that Clik1 represents a novel regulator of actin stress fibers...
  13. pmc Plant two-component signaling systems and the role of response regulators
    Jens Lohrmann
    Universitat Freiburg, Institut für Biologie II Botanik, Schanzlestrasse 1, 79104 Freiburg, Germany
    Plant Physiol 128:363-9. 2002
  14. pmc An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins
    P Bork
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Proc Natl Acad Sci U S A 89:7290-4. 1992
    ..A common evolutionary origin for all of the proteins in this class is proposed...
  15. pmc Phosphoproteomic analysis reveals interconnected system-wide responses to perturbations of kinases and phosphatases in yeast
    Bernd Bodenmiller
    Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland
    Sci Signal 3:rs4. 2010
    ..Our results serve to constrain future models of cellular signaling and reinforce the idea that simple linear representations of signaling pathways might be insufficient for drug development and for describing organismal homeostasis...
  16. ncbi Selenoprotein biosynthesis: purification and assay of components involved in selenocysteine biosynthesis and insertion in Escherichia coli
    Martin Thanbichler
    Institute of Genetics and Microbiology, University of Munich, D 80638 Munich, Germany
    Methods Enzymol 347:3-16. 2002
  17. ncbi Kinetic models of phosphorylation cycles: a systematic approach using the rapid-equilibrium approximation for protein-protein interactions
    Carlos Salazar
    Theoretische Biophysik, Institut fur Biologie, Humboldt Universitat zu Berlin, Invalidenstr 42, D 10115 Berlin, Germany
    Biosystems 83:195-206. 2006
    ..The approach outlined here may be useful also for analysing more complex systems, including multiple phosphorylation and kinase cascades...
  18. doi Strategies for kinome profiling in cancer and potential clinical applications: chemical proteomics and array-based methods
    Sander R Piersma
    Department of Medical Oncology, OncoProteomics Laboratory, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands
    Anal Bioanal Chem 397:3163-71. 2010
    ....
  19. ncbi A new family of phosphotransferases with a P-loop motif
    Anne Galinier
    Laboratoire de Chimie Bacterienne, UPR 9043, Institut de Biologie Structurale et Microbiologie CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille, France
    J Biol Chem 277:11362-7. 2002
    ..and phosphoenolpyruvate carboxykinase active sites supports the view that both enzymes bear significant resemblance in their overall mechanism of functioning showing that these two enzymes constitute a new family of phosphotransferases.
  20. pmc Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids
    C J Thompson
    Proc Natl Acad Sci U S A 80:5190-4. 1983
    ..fradiae aph with the aph genes encoded by bacterial transposons Tn5 and Tn903 showed significant nucleotide and amino acid homologies which indicated a common evolutionary origin for these antibiotic-resistance genes...
  21. ncbi Sulfate-activating enzymes of Penicillium chrysogenum. The ATP sulfurylase.adenosine 5'-phosphosulfate complex does not serve as a substrate for adenosine 5'-phosphosulfate kinase
    F Renosto
    Department of Biochemistry and Biophysics, University of California, Davis 95616
    J Biol Chem 264:9433-7. 1989
    ..The cumulative results suggest that the two sulfate activating enzymes do not associate to form a "3'-phosphoadenosine 5'-phosphosulfate synthetase" complex...
  22. doi ADP-Glo: A Bioluminescent and homogeneous ADP monitoring assay for kinases
    Hicham Zegzouti
    Promega Corporation, Madison, Wisconsin 53711, USA
    Assay Drug Dev Technol 7:560-72. 2009
    ..Due to its high signal to background and luminescent readout, this assay is less susceptible to generation of false hits and thus it is applicable to not only primary and secondary screening but also kinase profiling...
  23. pmc ArcS, the cognate sensor kinase in an atypical Arc system of Shewanella oneidensis MR-1
    Jürgen Lassak
    Department of Ecophysiology, Max Planck Institut fur terrestrische Mikrobiologie, Marburg, Germany
    Appl Environ Microbiol 76:3263-74. 2010
    ..Thus, we speculate that ArcS might have adopted the role of ArcB after a loss of the original sensor kinase, perhaps as a consequence of regulatory adaptation to a redox-stratified environment...
  24. ncbi Characterization of two novel regulatory genes affecting Salmonella invasion gene expression
    C Altier
    Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    Mol Microbiol 35:635-46. 2000
    ....
  25. doi Sixteen-kinase gene expression identifies luminal breast cancers with poor prognosis
    Pascal Finetti
    UMR599 Inserm, Institut Paoli Calmettes, Laboratoire d Oncologie Moleculaire, Centre de Recherche en Cancerologie de Marseille, Marseille, France
    Cancer Res 68:767-76. 2008
    ..The identification of luminal A cases of poor prognosis should help select appropriate treatment, whereas the identification of a relevant kinase set provides potential targets...
  26. ncbi Peptide arrays for kinase profiling
    Mike Schutkowski
    JPT Peptide Technologies GmbH, Invalidenstrasse 130, 10115 Berlin, Germany
    Chembiochem 6:513-21. 2005
  27. pmc Cyclin-dependent kinase 5 activity regulates pain signaling
    Tej K Pareek
    Functional Genomics Section, Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:791-6. 2006
    ..In conclusion, our data demonstrate a role for Cdk5/p35 activity in primary afferent nociceptive signaling, suggesting that Cdk5/p35 may be a target for the development of analgesic drugs...
  28. pmc Large-scale profiling of Rab GTPase trafficking networks: the membrome
    Cemal Gurkan
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 16:3847-64. 2005
    ..An interactive website for exploring datasets comprising components of the Rab-regulated hubs that define the membrome of different cell and organ systems in both human and mouse is available at http://www.membrome.org/...
  29. ncbi Down-regulation of pyrophosphate: fructose 6-phosphate 1-phosphotransferase (PFP) activity in sugarcane enhances sucrose accumulation in immature internodes
    Jan Hendrik Groenewald
    Institute for Plant Biotechnology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
    Transgenic Res 17:85-92. 2008
    ..Moreover, it suggests a role for PFP in glycolytic carbon flow, which could be rate limiting under conditions of high metabolic activity...
  30. ncbi Maltose utilization in Enterococcus faecalis
    Y Le Breton
    Laboratoire de Microbiologie de l Environnement, USC INRA, IRBA, Universite de Caen, 14032 Caen Cedex, France
    J Appl Microbiol 98:806-13. 2005
    ..The aim of this research was to characterize the metabolic pathway for maltose utilization in Enterococcus faecalis...
  31. ncbi Polyphosphate kinase from Escherichia coli. Purification and demonstration of a phosphoenzyme intermediate
    K Ahn
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307
    J Biol Chem 265:11734-9. 1990
    ..The basis for this stimulatory effect is not clear inasmuch as tetrapolyphosphate does not promote the dephosphorylation of the presumed phosphoenzyme intermediate...
  32. doi Prediction of response to preoperative chemoradiotherapy in rectal cancer by multiplex kinase activity profiling
    Sigurd Folkvord
    Department of Tumor Biology, Norwegian Radium Hospital, Oslo University Hospital, Norway
    Int J Radiat Oncol Biol Phys 78:555-62. 2010
    ..This study aimed to determine whether tumor kinase activity profiles might predict tumor response to preoperative CRT in locally advanced rectal cancer (LARC)...
  33. pmc The Escherichia coli BarA-UvrY two-component system is needed for efficient switching between glycolytic and gluconeogenic carbon sources
    Anna Karin Pernestig
    Microbiology and Tumorbiology Center, Karolinska Institutet, SE 17177 Stockholm, Sweden
    J Bacteriol 185:843-53. 2003
    ..Taking these results together, we propose the BarA-UvrY two-component system is crucial for efficient adaptation between different metabolic pathways, an essential function for adaptation to a new environment...
  34. pmc A combination of multisite phosphorylation and substrate sequestration produces switchlike responses
    Xinfeng Liu
    Department of Mathematics, University of South Carolina, Columbia, South Carolina, USA
    Biophys J 98:1396-407. 2010
    ..In some strategies, such as when phosphorylation and dephosphorylation are segregated, a near-optimal switch is possible, where the effective Hill number equals the number of phosphorylation sites...
  35. pmc Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
    R Lynn Sherrer
    Department of Molecular Biophysics, Yale University, New Haven, CT 06520 8114, USA
    Nucleic Acids Res 36:1247-59. 2008
    ..Phylogenetic analysis of PSTK in the context of its 'DxTN' kinase family shows that PSTK co-evolved precisely with SepSecS and indicates the presence of a previously unidentified PSTK in Plasmodium species...
  36. pmc Protein kinase Czeta regulates Cdk5/p25 signaling during myogenesis
    Aurelie de Thonel
    Turku Centre for Biotechnology, University of Turku and Abo Akademi University, 20521 Turku, Finland
    Mol Biol Cell 21:1423-34. 2010
    ....
  37. ncbi The role of the Rcs phosphorelay in Enterobacteriaceae
    Ya Hui Huang
    Molecular Microbiology Laboratory, Department of Biology and Biochemistry, University of Bath, UK
    Res Microbiol 157:206-12. 2006
    ..In this review we discuss the role of the Rcs phosphorelay in the Enterobacteriaceae, highlighting the observation that the Rcs phosphorelay appears to play a key role in the temporal regulation of biofilm formation and pathogenicity...
  38. ncbi Evolution and classification of P-loop kinases and related proteins
    Detlef D Leipe
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    J Mol Biol 333:781-815. 2003
    ..The results could serve as a framework for systematic experimental characterization of new biochemical and biological functions of kinases...
  39. doi Non-small-cell lung cancer harbouring mutations in the EGFR kinase domain
    Rafael Rosell
    Catalan Institute of Oncology, Hospital Germans Trias i Pujol, Badalona, Barcelona, Spain
    Clin Transl Oncol 12:75-80. 2010
    ..The sensitivity to EGFR TKIs could be greatly influenced by the expression of genes involved in the repair of DNA double-strand breaks by homologous recombination and non-homologous end joining...
  40. ncbi Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance
    Jeffrey P Mackeigan
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Cell Biol 7:591-600. 2005
    ..Finally, RNAi targeting of specific protein kinases sensitizes resistant cells to chemotherapeutic agents. The development of inhibitors that target these kinases or phosphatases may lead to new anti-cancer strategies...
  41. pmc A novel device of bacterial signal transducers
    K Ishige
    Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan
    EMBO J 13:5195-202. 1994
    ..These findings suggest that this unique feature of ArcB and BarA, in terms of the signaling modules, make it possible for these sensory kinases to function as dual-signaling transducers...
  42. ncbi A new class of phosphotransferases phosphorylated on an aspartate residue in an amino-terminal DXDX(T/V) motif
    J F Collet
    Laboratory of Physiological Chemistry, Christian de Duve Institute of Cellular Pathology and Catholic University of Louvain, B 1200 Brussels, Belgium
    J Biol Chem 273:14107-12. 1998
    ..In conclusion, phosphomannomutase and L-3-phosphoserine phosphatase belong to a new phosphotransferase family with an amino-terminal DXDX(T/V) motif that serves as an intermediate phosphoryl acceptor...
  43. pmc Pathogenic forms of tau inhibit kinesin-dependent axonal transport through a mechanism involving activation of axonal phosphotransferases
    Nicholas M Kanaan
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Neurosci 31:9858-68. 2011
    ..Results from these studies reveal a novel role for tau in modulating axonal phosphotransferases and provide a molecular basis for a toxic gain-of-function associated with pathogenic forms of tau.
  44. ncbi Sequence of a putative glucose 6-phosphate translocase, mutated in glycogen storage disease type Ib
    I Gerin
    Laboratory of Physiological Chemistry, Institute of Cellular Pathology and University of Louvain, Brussels, Belgium
    FEBS Lett 419:235-8. 1997
    ..The encoded protein is therefore most likely the glucose 6-phosphate translocase that is functionally associated with glucose-6-phosphatase...
  45. pmc The Arabidopsis histidine phosphotransfer proteins are redundant positive regulators of cytokinin signaling
    Claire E Hutchison
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Plant Cell 18:3073-87. 2006
    ..These data indicate that most of the AHPs are redundant, positive regulators of cytokinin signaling and affect multiple aspects of plant development...
  46. doi The subcellular distribution of the Arabidopsis histidine phosphotransfer proteins is independent of cytokinin signaling
    Jayson A Punwani
    Department of Biology, University of North Carolina, CB 3280, Chapel Hill, NC 27599 3280, USA
    Plant J 62:473-82. 2010
    ..Rather, AHPs actively maintain a consistent nuclear/cytosolic distribution regardless of the status of the cytokinin response pathway...
  47. doi FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region
    Toshiyasu Koike
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 421:157-62. 2009
    ..These results suggest that FAM20B regulates the number of glycosaminoglycan chains by phosphorylating the xylose residue in the glycosaminoglycan-protein linkage region of proteoglycans...
  48. doi Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle
    Henrik Daub
    Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 31:438-48. 2008
    ..These results provide a vastly extended knowledge base for functional studies on kinases and their regulation through site-specific phosphorylation...
  49. pmc Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry
    An Chi
    Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA
    Proc Natl Acad Sci U S A 104:2193-8. 2007
    ..They are also likely to be conserved across large evolutionary distances...
  50. pmc The BarA-UvrY two-component system regulates virulence in avian pathogenic Escherichia coli O78:K80:H9
    Christopher D Herren
    Virginia Maryland Regional College of Veterinary Medicine, University of Maryland at College Park, 8075 Greenmead Drive, College Park, Maryland 20742 3711, USA
    Infect Immun 74:4900-9. 2006
    ..Our results indicate that BarA-UvrY regulates virulence properties in APEC and that the chicken embryo lethality assay can be used as a surrogate model to determine virulence determinants and their regulation in APEC strains...
  51. doi DNA damage during meiosis induces chromatin remodeling and synaptonemal complex disassembly
    Florence Couteau
    Department of Biology, McGill University, 1205 Avenue Doctor Penfield, Montreal, QC H3A 1B1, Canada
    Dev Cell 20:353-63. 2011
    ..We propose that the meiotic DDR involves early chromatin remodeling at chromosome axes to dismantle structures promoting interhomolog recombination and facilitate efficient nonhomolog-based repair before pachytene exit...
  52. ncbi Cables links Cdk5 and c-Abl and facilitates Cdk5 tyrosine phosphorylation, kinase upregulation, and neurite outgrowth
    L R Zukerberg
    Howard Hughes Medical Institute, Department of Pathology, Massachusetts General Hospital, Boston 02114, USA
    Neuron 26:633-46. 2000
    ..Furthermore, expression of active Abl resulted in lengthening of neurites. The data provide evidence for a Cables-mediated interplay between the Cdk5 and c-Abl signaling pathways in the developing nervous system...
  53. pmc An efficient rapid system for profiling the cellular activities of molecular libraries
    Jonathan S Melnick
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Proc Natl Acad Sci U S A 103:3153-8. 2006
    ..This methodology provides a powerful tool for unraveling the cellular biology and molecular pharmacology of both naturally occurring and synthetic chemical diversity...
  54. ncbi DIAP1 suppresses ROS-induced apoptosis caused by impairment of the selD/sps1 homolog in Drosophila
    Marta Morey
    Departament de Genetica, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
    J Cell Sci 116:4597-604. 2003
    ..These observations indicate that selDptuf ROS-induced apoptosis in Drosophila is mainly driven by the caspase-dependent Dmp53/Rpr pathway...
  55. doi Trends in kinase selectivity: insights for target class-focused library screening
    Shana L Posy
    Computer Assisted Drug Design, Applied Biotechnology, Bristol Myers Squibb Research and Development, Princeton, New Jersey 08543, United States
    J Med Chem 54:54-66. 2011
    ..Our results support a scaffold-oriented approach for building compound collections to screen kinase targets...
  56. ncbi The putative glucose 6-phosphate translocase gene is mutated in essentially all cases of glycogen storage disease type I non-a
    M Veiga-da-Cunha
    Laboratory of Physiological Chemistry, Brussels, Belgium
    Eur J Hum Genet 7:717-23. 1999
    ..Isolated defects in microsomal Pi transporter or in microsomal glucose transporter must be very rare or have phenotypes that are not recognised as GSD I, so that in practice there are only two subtypes of GSD I (GSD Ia and GSD Ib)...
  57. pmc An enriched structural kinase database to enable kinome-wide structure-based analyses and drug discovery
    Natasja Brooijmans
    Chemical Sciences, Wyeth Research, Pearl River, New York 10965, USA
    Protein Sci 19:763-74. 2010
    ..Furthermore, 90% of kinases are found to have at least one stabilizing interaction for the hinge region, which has not been described before...
  58. ncbi Microbial genome analyses: comparative transport capabilities in eighteen prokaryotes
    I T Paulsen
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    J Mol Biol 301:75-100. 2000
    ..Archaea and spirochetes exhibit fewer proteins with multiple transmembrane segments and fewer net transporters than most bacteria. These results provide insight into the relevance of transport to the overall physiology of prokaryotes...
  59. ncbi patufet, the gene encoding the Drosophila melanogaster homologue of selenophosphate synthetase, is involved in imaginal disc morphogenesis
    B Alsina
    Departament de Genetica, Universitat de Barcelona, Spain
    Mol Gen Genet 257:113-23. 1998
    ..The synthesis of selenoproteins by the selenophosphate synthetase, the role of selenoproteins in the maintenance of the oxidant/antioxidant balance of the cell and its possible implications in imaginal disc morphogenesis are discussed...
  60. pmc Elevation of glutamine level by selenophosphate synthetase 1 knockdown induces megamitochondrial formation in Drosophila cells
    Myoung Sup Shim
    Laboratory of Molecular Genetics and Genomics, School of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 284:32881-94. 2009
    ....
  61. pmc Human selenophosphate synthetase 1 has five splice variants with unique interactions, subcellular localizations and expression patterns
    Jin Young Kim
    Laboratory of Molecular Genetics and Genomics, School of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151 742, Republic of Korea
    Biochem Biophys Res Commun 397:53-8. 2010
    ..DeltaE2 expression peaked at mid-S phase and then gradually decreased. However, +E9/+E9a expression decreased gradually after cell cycle arrest. The possible involvement of SPS1 splice variants in cell cycle regulation is discussed...
  62. doi PtdIns5P regulation through evolution: roles in membrane trafficking?
    Odile Lecompte
    Department of Structural Biology and Genomics, rue Laurent Fries, Illkirch, F 67400 France
    Trends Biochem Sci 33:453-60. 2008
    ..By examining PtdIns5P metabolism in this new perspective, we propose a role for PtdIns5P in membrane trafficking from late endosomal compartments to the plasma membrane...
  63. ncbi Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli
    A Brinkkötter
    Universitat Osnabruck, Fachbereich Biologie chemie, D 49069 Osnabruck, Germany
    Mol Microbiol 37:125-35. 2000
    ..coli K-12. These retain the DeltaagaW' EF 'A deletion and carry suppressor mutations in the gat and nag genes for galactitol and N-acetyl-glucosamine metabolism, respectively, that allow growth on Aga but not on GalN...
  64. ncbi Prenatal diagnosis of glycogen storage disease type 1b using denaturing high performance liquid chromatography
    C W Lam
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, China
    Prenat Diagn 20:765-8. 2000
    ..We show here that DNA mutation analysis can be used in the prenatal diagnosis of GSD1b and that DHPLC promises to be a robust technique for this and other prenatal molecular diagnoses...
  65. ncbi Phosphorylation-induced transient intrinsic structure in the kinase-inducible domain of CREB facilitates its recognition by the KIX domain of CBP
    Iván Solt
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest, Hungary
    Proteins 64:749-57. 2006
    ....
  66. ncbi Formation of a novel four-helix bundle and molecular recognition sites by dimerization of a response regulator phosphotransferase
    K I Varughese
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA
    Mol Cell 2:485-93. 1998
    ..It is apparent that different surfaces of response regulators may be involved in recognition of the protein partners to which they are paired...
  67. pmc The Escherichia coli BarA-UvrY two-component system is a virulence determinant in the urinary tract
    Henrik Tomenius
    Swedish Institute for Infectious Disease Control, SE 17182 Solna, Sweden
    BMC Microbiol 6:27. 2006
    ..Our aim was to determine if the Escherichia coli BarA-UvrY two-component system is connected with virulence using a monkey cystitis model...
  68. pmc Identification of a novel selD homolog from eukaryotes, bacteria, and archaea: is there an autoregulatory mechanism in selenocysteine metabolism?
    M J Guimaraes
    Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA
    Proc Natl Acad Sci U S A 93:15086-91. 1996
    ..This mechanism is likely to have been present in ancient life forms and conserved in a variety of living organisms from all domains of life...
  69. pmc A KaiC-associating SasA-RpaA two-component regulatory system as a major circadian timing mediator in cyanobacteria
    Naoki Takai
    Division of Biological Science, Graduate School of Science, Nagoya University, Furocho, Nagoya 464 8602, Japan
    Proc Natl Acad Sci U S A 103:12109-14. 2006
    ..Moreover, our results indicate the presence of secondary output pathways from the clock to transcription control, suggesting that multiple pathways ensure a genome-wide circadian system...
  70. pmc Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database
    Thomas S Nühse
    Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, United Kingdom
    Plant Cell 16:2394-405. 2004
    ..All data are deposited in a new searchable database for plant phosphorylation sites maintained by PlantsP (http://plantsp.sdsc.edu) that will be updated as the project expands to encompass additional tissues and organelles...
  71. ncbi Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis
    S C Low
    Thyroid Division, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 270:21659-64. 1995
    ..Further analysis revealed that reduced selenium labeling was due to altered ATP binding properties of the mutant selenophosphate synthetases...
  72. ncbi Current in vitro kinase assay technologies: the quest for a universal format
    Yong Jia
    Abbott Laboratories, 100 Research Drive, Worcester, MA 01605, USA
    Curr Drug Discov Technol 5:59-69. 2008
    ..Several factors, including the intended applications, cost, timeline, expertise, familiarity, and comfort level, should be considered prior to pursuing a particular kinase assay technology...
  73. pmc Fetal mouse selenophosphate synthetase 2 (SPS2): characterization of the cysteine mutant form overproduced in a baculovirus-insect cell system
    I Y Kim
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 94:418-21. 1997
    ....
  74. ncbi Inverse metabolic engineering with phosphagen kinase systems improves the cellular energy state
    Uwe Sauer
    Institute of Biotechnology, Swiss Federal Institute of Technology ETH Zurich, CH 8093
    Metab Eng 6:220-8. 2004
    ..Examples are discussed that illustrate how microbial metabolism can be tailored for large-scale industrial processes with imperfect mixing and how the liver can be protected from metabolic insults or stimulated for better regeneration...
  75. ncbi AHP2 is required for bivalent formation and for segregation of homologous chromosomes in Arabidopsis meiosis
    Carla Schommer
    Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK
    Plant J 36:1-11. 2003
    ..Our results suggest that meu13+ function is conserved in higher eukaryotes and support the idea that Arabidopsis, yeast and mouse share a pairing pathway that is not present in Drosophila melanogaster and Caenorhabditis elegans...
  76. pmc Cilia and the cell cycle?
    Lynne M Quarmby
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    J Cell Biol 169:707-10. 2005
    ..Work from the unicellular alga Chlamydomonas is providing novel insights as to how cilia and the cell cycle are coordinately regulated...
  77. ncbi Screening of substrate analogs as potential enzyme inhibitors for the arginine kinase of Trypanosoma cruzi
    Claudio A Pereira
    Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
    J Eukaryot Microbiol 50:132-4. 2003
    ..The development of the arginine kinase inhibitors of T. cruzi could be an important feature because the phosphagens biosynthetic pathway in trypanosomatids is different from the one in their mammalian hosts...
  78. ncbi Selenocysteine-containing proteins in mammals
    V N Gladyshev
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
    J Biomed Sci 6:151-60. 1999
    ..This review describes known mammalian selenoproteins and discusses recent developments and future directions in the selenium field...
  79. ncbi Regulation of vertebrate cellular Mg2+ homeostasis by TRPM7
    Carsten Schmitz
    Department of Pediatrics, University of Washington and Children s Hospital and Regional Medical Center, Seattle, WA 98195, USA
    Cell 114:191-200. 2003
    ..Overall, our results indicate that TRPM7 has a central role in Mg(2+) homeostasis as a Mg(2+) uptake pathway regulated through a functional coupling between its channel and kinase domains...
  80. ncbi Regulation of gene expression in the PTS in Escherichia coli: the role and interactions of Mlc
    Jacqueline Plumbridge
    Institut de Biologie Physico Chimique, UPR9073, 13 rue Pierre et Marie Curie, 75005, Paris, France
    Curr Opin Microbiol 5:187-93. 2002
    ..PtsG is, thus, subject to multiple layers of control that, in turn, feed back on the regulation of the PTS by Mlc. The regulatory function of Mlc is compared with that of FruR and of the PTS-regulation-domain-containing protein, BglG...
  81. pmc The dihydroxyacetone kinase of Escherichia coli utilizes a phosphoprotein instead of ATP as phosphoryl donor
    R Gutknecht
    Departement fur Chemie und Biochemie, Universitat Bern, Freiestrasse 3, CH 3012 Bern, Switzerland
    EMBO J 20:2480-6. 2001
    ..DhaK and DhaL are not phosphorylated. The IIA domain of DhaM, instead of ATP, is the phosphoryl donor to dihydroxyacetone (Dha). Unlike the carbohydrate-specific transporters of the PTS, DhaK, DhaL and DhaM have no transport activity...
  82. ncbi The relationship between external glucose concentration and cAMP levels inside Escherichia coli: implications for models of phosphotransferase-mediated regulation of adenylate cyclase
    L Notley-McRobb
    Department of Microbiology G08, University of Sydney, New South Wales, Australia
    Microbiology 143:1909-18. 1997
    ..3 mM, a second shift occurred below 1 muM glucose. Approaching starvation, well below saturation of the PTS, cAMP levels either increased or decreased depending on unknown factors that differ between common E. coli K-12 strains...
  83. ncbi The thrH gene product of Pseudomonas aeruginosa is a dual activity enzyme with a novel phosphoserine:homoserine phosphotransferase activity
    S Kumar Singh
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 279:13166-73. 2004
    ....
  84. pmc Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase
    Bradley A Carlson
    Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:12848-53. 2004
    ..The fact that PSTK has been highly conserved in evolution suggests that it plays an important role in selenoprotein biosynthesis and/or regulation...
  85. ncbi The kinase homology domain of receptor guanylyl cyclase C: ATP binding and identification of an adenine nucleotide sensitive site
    Mahaboobi Jaleel
    Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India
    Biochemistry 45:1888-98. 2006
    ....
  86. ncbi Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes
    C Clepet
    Laboratoire de Chimie Bacterienne, Centre National de la Recherche Scientifique, Marseille, France
    Mol Microbiol 6:3109-19. 1992
    ..HDH activity was feedback inhibited by threonine; the expression of all three genes was constitutive. The overall organization of these three genes appears to differ from that in other bacterial species...
  87. pmc Xylitol and D-arabitol toxicities due to derepressed fructose, galactitol, and sorbitol phosphotransferases of Escherichia coli
    A M Reiner
    J Bacteriol 132:166-73. 1977
    ....
  88. pmc Selenophosphate synthetase 2 is essential for selenoprotein biosynthesis
    Xue Ming Xu
    Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem J 404:115-20. 2007
    ..These in vivo studies indicate that SPS2 is essential for generating the selenium donor for selenocysteine biosynthesis in mammals, whereas SPS1 probably has a more specialized, non-essential role in selenoprotein metabolism...
  89. ncbi Molecular basis for positive and negative signaling by the natural killer cell receptor 2B4 (CD244)
    Philipp Eissmann
    Institute for Immunology, University Heidelberg, Germany
    Blood 105:4722-9. 2005
    ..We now show that Fyn can also associate with phosphorylated 2B4. Finally, we demonstrate that Fyn and Csk can both phosphorylate 2B4, suggesting a possible mechanism of 2B4 phosphorylation...
  90. ncbi Specificity in two-component signal transduction pathways
    Michael T Laub
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Annu Rev Genet 41:121-45. 2007
    ....
  91. ncbi Processive phosphorylation of p130Cas by Src depends on SH3-polyproline interactions
    P Pellicena
    Department of Physiology and Biophysics, School of Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794-8661, USA
    J Biol Chem 276:28190-6. 2001
    ..Second, this region aids in anchoring the kinase to Cas to facilitate processive phosphorylation of the substrate domain. The two processes combine to ensure phosphorylation of Cas with high efficiency...
  92. pmc Metal ion-mediated polymer superquenching for highly sensitive detection of kinase and phosphatase activities
    Frauke Rininsland
    QTL Biosystems, 2778 Agua Fria Street, Santa Fe, NM 87507, USA
    Proc Natl Acad Sci U S A 101:15295-300. 2004
    ..The assay meets the sensitivity required for high-throughput screening of kinase or phosphatase inhibitors and is a valuable tool for drug discovery. A modified version of the assay allows for the detection of protein phosphorylation...
  93. ncbi Specificity of the BvgAS and EvgAS phosphorelay is mediated by the C-terminal HPt domains of the sensor proteins
    A L Perraud
    Lehrstuhl fur Mikrobiologie, Theodor Boveri Institut, Biozentrum der Universität Würzburg, Germany
    Mol Microbiol 27:875-87. 1998
    ....
  94. ncbi Evolutionary relationships between sugar kinases and transcriptional repressors in bacteria
    F Titgemeyer
    Department of Biology, University of California at San Diego, La Jolla, CA 92093 0116
    Microbiology 140:2349-54. 1994
    ....
  95. ncbi A trans acting ribozyme that phosphorylates exogenous RNA
    Dayal Saran
    Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, Indiana 47405, USA
    Biochemistry 44:15007-16. 2005
    ..Lack of reactivity in [Co(NH(3))(6)](3+) indicates a requirement for inner sphere contact with the metal ion, either for structural stabilization, catalysis, or both...
  96. pmc Comparative genomic analyses of the bacterial phosphotransferase system
    Ravi D Barabote
    Division of Biological Sciences, University of California at San Diego, La Jolla, California 92093 0116, USA
    Microbiol Mol Biol Rev 69:608-34. 2005
    ..Our studies provide insight into the development of complex multicomponent enzyme systems and lead to predictions regarding the types of protein-protein interactions that promote efficient PTS-mediated phosphoryl transfer...
  97. pmc Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue
    J P J Saeij
    Department of Microbiology and Immunology, Fairchild Building D305, 300 Pasteur Drive, Stanford University School of Medicine, Stanford, California 94305 5124, USA
    Nature 445:324-7. 2007
    ..Our findings provide a new mechanism for how an intracellular eukaryotic pathogen can interact with its host and reveal important differences in how different Toxoplasma lineages have evolved to exploit this interaction...
  98. ncbi Structure and mutation analysis of the glycogen storage disease type 1b gene
    P Marcolongo
    Institute of General Pathology, University of Siena, Italy
    FEBS Lett 436:247-50. 1998
    ..Primers based on the genomic sequence were used in single strand conformation polymorphism (SSCP) analysis and mutations were found in six out of seven GSD 1b patients analysed...
  99. doi PhosphoPOINT: a comprehensive human kinase interactome and phospho-protein database
    Chia Ying Yang
    Department of Computer Science and Information Engineering, National Taiwan University, Taipei 106, Taiwan, Republic of China
    Bioinformatics 24:i14-20. 2008
    ..In contrast, 85% of the kinases have protein-protein interaction (PPI) datasets, raising the possibility that we might reveal potential kinase-substrate pairs from these PPIs...
  100. ncbi Differential activation of the DNA replication checkpoint contributes to asynchrony of cell division in C. elegans embryos
    Michael Brauchle
    ISREC Swiss Institute for Experimental Cancer Research, 155, Ch des Boveresses, CH 1066 Epalinges Lausanne, Switzerland
    Curr Biol 13:819-27. 2003
    ..How anterior-posterior (A-P) polarity cues control this asynchrony remains to be elucidated...
  101. ncbi Mechanisms of drug inhibition of signalling molecules
    Judith S Sebolt-Leopold
    Pfizer Global Research and Development, Michigan Laboratories, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA
    Nature 441:457-62. 2006
    ..However, despite uncertainty over precise or dominant mechanisms of action, especially for compounds targeting multiple gene products, emerging agents are producing significant therapeutic advances against a broad range of human cancers...

Research Grants74

  1. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  2. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
    ....
  3. Lauren Endres; Fiscal Year: 2014
    ..Overall, these findings will form the basis of future studies into Abh8 regulation during the DDR. ..
  4. Xenoestrogen regulation of Leydig cells
    BENSON TOKUNBO AKINGBEMI; Fiscal Year: 2010
    ..Research training to be afforded by the present application to professional veterinary students will contribute to efforts geared to preparing future veterinarians to meet these challenges. ..
  5. Pyruvate Dehydrogenase kinase as Therapeutic Target for Hepatic Steatosis, Obesit
    Robert A Harris; Fiscal Year: 2012
    ..We therefore believe the proposed work has the potential to have a significant impact on the health care of Veterans. ..
  6. SHAOCHUNG VICTOR HSIA; Fiscal Year: 2016
    ..The long-term goal is to identify the regulatory mechanisms to assist in the development of novel therapeutic protocols for better treatments. ..
  7. Yun Fai Chris Lau; Fiscal Year: 2014
    ..This project will shed important insights on how the Y-oncogene and X-tumor suppressor operate and how one can interfere or boost their actions respectively to treat prostate cancer. ..
  8. Cory Teuscher; Fiscal Year: 2014
    ....
  9. Role of Chromogranin A in Metabolic Syndrome
    Sushil K Mahata; Fiscal Year: 2013
    ..2. Evaluate the potential therapeutic effects of CST and its variants on insulin sensitivity and baroreflex sensitivity and heart rate variability in high fat diet-induced insulin resistant and in db/db diabetic mice. ..
  10. VINCENT SCOTT TAGLIABRACCI; Fiscal Year: 2014
    ....
  11. FASEB SRC on Lysophospholipd Mediators in Health and Disease
    Julie D Saba; Fiscal Year: 2011
    ....
  12. Ang II and Aldosterone Effects on Insulin Resistance in Cardiovascular Tissue
    JAMES RUSSELL SOWERS; Fiscal Year: 2013
    ..A better understanding of factors involved in insulin resistance should enable development of therapeutic targets to help prevent diabetes and cardiovascular disease. ..
  13. Emilio B Gonzalez; Fiscal Year: 2014
    ..These studies may help to devise new more specific and less harmful modalities than the anticoagulation or general immunosuppression currently used to treat and prevent thrombosis in patients with aPL Abs. ..
  14. CLIFFORD RUSSELL GREYSON; Fiscal Year: 2016
    ..Agents with potential clinical utility will be identified using the isolated papillary muscle model and tested in the pig model. ..
  15. JAMES RUSSELL SOWERS; Fiscal Year: 2015
    ..A better understanding of factors involved in insulin resistance should help in the development of therapeutic targets to help prevent diabetes and cardiovascular disease. ..
  16. Role of Txnip in adapting fuel metabolism to nutritional state
    SIMON HUI; Fiscal Year: 2013
    ..Results of this proposed research will provide fundamental information that may lead to better prevention and/or therapeutics for diabetes and cardiovascular disease in humans. ..
  17. Agrin/alpha 3 Na,K-ATPase signaling at the neuromuscular junction
    Martin A Smith; Fiscal Year: 2012
    ..This novel signal pathway represents a new therapeutic target for the treatment of neuromuscular disease. ..
  18. Sphingolipid Metabolism in Drosophila Development
    Julie D Saba; Fiscal Year: 2013
    ..Thus, our research plan has potentially broad translational significance. ..
  19. Stephen E McGowan; Fiscal Year: 2016
    ..These studies are relevant to other vascular diseases including stroke, diabetes, and chronic renal insufficiency, which are also common among veterans. ..
  20. Randal J Kaufman; Fiscal Year: 2014
    ..The proposed studies on protein synthesis will provide vital information that should encourage the development of novel therapeutics to treat diabetes. ..
  21. Inhibition of human neutrophil function by Francisella tularensis
    Lee Ann H Allen; Fiscal Year: 2012
    ..The results of this study may lead to new treatments to combat this severe and often fatal lung infection. ..
  22. Cellular &Inflammatory Pathways in Ashtma &Allergic Diseases: From IgE to Cells
    LAWRENCE BARRY SCHWARTZ; Fiscal Year: 2012
    ..Understanding more precisely the characteristics of and the mechanism(s) behind desensitization will enable physicians to better utilize this approach to reduce mast cell/basophil-mediated contributions to asthma and allergic diseases. ..
  23. REGULATION AND FUNCTION OF STE20-RELATED PROTEIN KINASES
    Melanie H Cobb; Fiscal Year: 2012
    ..Understanding the mechanisms of action of this family will provide significant insights into the control of ion balance and other essential processes, and may reveal novel strategies to develop anti-hypertensive therapies. ..
  24. Michael Demetriou; Fiscal Year: 2014
    ..Here we further explore the mechanisms by which protein glycosylation regulates immune function and autoimmunity. ..
  25. Bayar Thimmapaya; Fiscal Year: 2016
    ....
  26. N-methanocarbathymidine (N-MCT) for the Treatment of Herpes and Pox Virus Infecti
    Aquilur Rahman; Fiscal Year: 2010
    ....
  27. Patient-Derived Stem Cells for Phosphoproteomic Profiling Neuropsychopathology
    Dieter A Wolf; Fiscal Year: 2011
    ....
  28. Translational Machinery in Stress Signaling and Tumor Suppression
    THOMAS J contact BEGLEY; Fiscal Year: 2010
    ..The long term potential of the proposed research is significant, as understanding the mechanisms by which TRM9 inhibits tumor growth may provide novel therapeutic opportunities to restore growth-restricting programs in cancer cells. ..
  29. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
    ..Our research will be useful in the development of novel treatments for the most aggressive forms of the childhood cancer neuroblastoma. ..
  30. Genetic and physiological causes of inherited Vascular and Metabolic Diseases
    Arya Mani; Fiscal Year: 2013
    ..The current study goals are investigating the effect of the mutation on metabolism of glucose and insulin secretion and function in patients with this mutation. ..
  31. Mitchell Kronenberg; Fiscal Year: 2014
    ..This proposal will study the mechanisms by which the immune responses are properly controlled. Such knowledge will eventually facilitate the design of novel therapeutic approaches for immunological diseases. ..
  32. Trichothecene Toxicity and the Ribotoxic Stress Response
    James J Pestka; Fiscal Year: 2012
    ..Collectively, these outcomes will positively impact public health by providing a scientific basis for generating sound recommendations relative to these important toxins and appropriate remedial actions should exposures occur. ..
  33. David A Bernlohr; Fiscal Year: 2014
    ..If the hypothesis is proven to be correct, the study would be immediately translatable to human biology and afford a new view of how type 2 diabetes may be combated. ..
  34. Maria Diakonova; Fiscal Year: 2014
    ..Understanding how these proteins work together will help to design new therapeutic approaches and possibly drugs for treatment of different human diseases and breast cancer. ..
  35. Kazem M Azadzoi; Fiscal Year: 2016
    ..abstract_text> ..
  36. Inderjit Singh; Fiscal Year: 2014
    ....
  37. Tat and CDK9 on gene expression alterations coupled to HIV-1 Associated Dementia
    Xavier Grana; Fiscal Year: 2010
    ....
  38. MATTHEW PETER COGGINS; Fiscal Year: 2014
    ..abstract_text> ..
  39. SAMY LEWIZ HABIB; Fiscal Year: 2016
    ....
  40. DHAN V KALVAKOLANU; Fiscal Year: 2014
    ..Studies proposed in this application will investigate the regulation of an anti-metastatic gene, DAPK1, which is critical for tumor suppression and autophagic responses. ..
  41. John S Davis; Fiscal Year: 2014
    ..Considering the importance sex steroids in health and disease, we must have a better understanding of the mechanisms controlling the synthesis of steroid hormones. ..
  42. Junichi Sadoshima; Fiscal Year: 2016
    ..The knowledge obtained from this investigation should be useful for the development of better treatment for heart failure, ischemic injury and stem cell therapy. ..
  43. Balakuntalam S Kasinath; Fiscal Year: 2016
    ..Upstream regulators and downstream effectors of GSK 3beta in transcription and translation pathways involved in matrix protein synthesis will be explored in renal tissues as stated in Aim 1. ..
  44. Ethanol Regulation of Serum Glucocorticoid Kinase I
    BLAIR N COSTIN; Fiscal Year: 2012
    ..The work proposed here could shed light on molecular mechanisms underlying alcoholism and eventually have implications in the development of novel pharmacological agents for alcoholism or alcohol-related neurological disorders. ..
  45. DAVID MATTHEW KELLER; Fiscal Year: 2016
    ..This proposal will characterize a new target of cAMP signaling, microRNA-375, which has the potential to be used as a complementary therapy to enhance the efficacy of existing therapeutics that target the cAMP signaling pathway. ..
  46. Subhash C Pandey; Fiscal Year: 2014
    ....
  47. AYLIN RACHEL RODAN; Fiscal Year: 2015
    ..This project seeks to better understand the molecular mechanisms by which these cotransporters work and how they are regulated, to improve understanding and treatment of high blood pressure and disorders of acid-base balance. ..
  48. MARK L DELL'ACQUA; Fiscal Year: 2016
    ....
  49. Protein Nitration in the Placenta
    Leslie Myatt; Fiscal Year: 2012
    ....
  50. BONE MATRIX AND BONE RESORPTION
    Paul R Odgren; Fiscal Year: 2012
    ..This proposal aims to investigate new genes that regulate the formation and activity of osteoclasts to better understand bone biology in general, and potentially to find new ways to treat these widespread bone disorders. ..
  51. Mahin D Maines; Fiscal Year: 2015
    ..In addition, the enzyme activity is necessary for protection against the fatal "green jaundice". Our work has revealed a novel approach for development of therapeutic interventions in a range of human diseases. ..
  52. Haifei Shi; Fiscal Year: 2015
    ....
  53. The Relationship Between STK39, Salt Sensitivity, and HCTZ-induced BP Response
    YEN PEI CHRISTY CHANG; Fiscal Year: 2010
    ....
  54. ALTERED CARDIAC MYOCYTE SIGNALING IN DIABETIC MYOCARDIUM AND FUNCTIONAL SEQUELAE
    ANTHONY JUSTIN MUSLIN; Fiscal Year: 2010
    ..The analysis of the role of Akt2 in diabetic cardiomyopathy may lead to new therapies that may help to reduce the high mortality rate associated with diabetes. ..
  55. Mary K Sheats; Fiscal Year: 2016
    ....
  56. Lai Hua Xie; Fiscal Year: 2015
    ....
  57. JoAnn Sekiguchi; Fiscal Year: 2014
    ..abstract_text> ..
  58. Christie P Thomas; Fiscal Year: 2014
    ....
  59. Mechanisms of Endothelin 1-Induced Sickle Cell Pathology
    Alicia Rivera; Fiscal Year: 2011
    ..We plan to characterize mechanisms of ET-1-stimulated sickle red cell dehydration as a means to develop ET-1 receptor antagonists as a novel therapy in the treatment of sickle cell disease. ..
  60. James B Martins; Fiscal Year: 2014
    ..Similarly such drugs may be combined with other antioxidants to prevent dangerous heart rhythms. ..
  61. ER stress: role in myocyte apoptosis and myocardial remodeling
    Krishna Singh; Fiscal Year: 2013
    ..The proposed studies investigating the role of ER stress in cardiac myocyte apoptosis and myocardial remodeling may uncover novel therapeutic strategies for the treatment of heart failure. ..
  62. Alzheimer's Disease Therapeutic
    Trevor P Castor; Fiscal Year: 2012
    ..Alternatively, at this stage, Amylon will merge with a mid-tier public company or be acquired by a multinational pharmaceutical company. ..
  63. Therapeutic for Intracerebral Hemorrhage
    Dale Christensen; Fiscal Year: 2009
    ....
  64. ACTIONS OF CYCLIC GMP IN SMOOTH MUSCLE
    Thomas Lincoln; Fiscal Year: 1999
    ....
  65. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Clarence Chan; Fiscal Year: 1999
    ..An alternative explanation would be that the sli mutations lead to an overproduction of PP1. He suggests experiments to test all three hypotheses, as well as the cloning and characterization of the wild type sli genes. ..
  66. ANTIGENIC DETERMINANTS OF VARICELLA VIRUS
    Charles Grose; Fiscal Year: 2006
    ..These proposed VZV studies will provide further insight into the multiple functions of gE and the gE:gI complex. ..
  67. MEIOTIC CELL CYCLE REGULATION IN REPRODUCTION
    Stuart Ravnik; Fiscal Year: 2002
    ..CycAl, Cdkl, and Cdk2 expression will be determined using immunohistochemistry. Co-immunoprecipitated proteins will be examined as Aim 1. ..
  68. REGULATION OF PLASMA CELL MALIGNANCY BY INSULIN AND IGF
    GREGORY FREUND; Fiscal Year: 2000
    ..Freund has a unique opportunity to develop into an outstanding clinician and scientist. ..
  69. CALMODULIN KINASE II AND ION TRANSPORT IN THE KIDNEY
    Rochelle Hanley; Fiscal Year: 1992
    ..These experiments should provide new information on the regulation of this protein kinase in the renal proximal convoluted tubule...