Genomes and Genes
Summary: Recombinases involved in the rearrangement of immunity-related GENES such as IMMUNOGLOBULIN GENES and T-CELL RECEPTOR GENES.
Publications172 found, 100 shown here
- RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombinationM A Oettinger
Whitehead Institute for Biomedical Research, Cambridge, MA 02142
Science 248:1517-23. 1990..RAG-1 and RAG-2 might activate the expression of the V(D)J recombinase but, more likely, they directly participate in the recombination reaction...
- RAG1 core and V(D)J recombination signal sequences were derived from Transib transposonsVladimir V Kapitonov
Genetic Information Research Institute, Mountain View, California, USA
PLoS Biol 3:e181. 2005..We also suggest that the RAG2 protein was not encoded by ancient Transib transposons but emerged in jawed vertebrates as a counterpart of RAG1 necessary for the V(D)J recombination reaction...
- DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytesU Grawunder
University of Southern California School of Medicine, Norris Comprehensive Cancer Center, Department of Pathology, Los Angeles 90033, USA
Mol Cell 2:477-84. 1998..Hence, DNA ligase IV is the activity responsible for the ligation step in NHEJ and in V(D)J recombination...
- Unraveling V(D)J recombination; insights into gene regulationDavid Jung
Howard Hughes Medical Institute, The Children s Hospital, The CBR Institute for Biomedical Research, Inc, Harvard Medical School, Boston, MA 02115, USA
Cell 116:299-311. 2004....
- RAG1-DNA binding in V(D)J recombination. Specificity and DNA-induced conformational changes revealed by fluorescence and CD spectroscopyMihai Ciubotaru
Howard Hughes Medical Institute, Yale University School of Medicine, Section of Immunobiology, New Haven, Connecticut 06510, USA
J Biol Chem 278:5584-96. 2003..RSS-induced conformational changes of StrRAG1 may influence the interaction of RAG1 with RAG2 and synaptic complex formation...
- The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell developmentHong Erh Liang
Department of Molecular and Cell Biology, Division of Immunology, University of California, Berkeley 94720, USA
Immunity 17:639-51. 2002....
- B lineage-specific regulation of V(D)J recombinase activity is established in common lymphoid progenitorsLisa Borghesi
Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester 01655, USA
J Exp Med 199:491-502. 2004..As activity of the Erag enhancer is restricted to the B cell lineage, this provides the first molecular evidence for establishment of a lineage-specific transcription program in multipotent progenitors...
- Mechanisms of chromosomal translocations in B cell lymphomasR Kuppers
Institute of Cancer Genetics, Columbia University, New York, NY 10032, USA
Oncogene 20:5580-94. 2001....
- Impaired immunoglobulin gene rearrangement in mice lacking the IL-7 receptorA E Corcoran
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
Nature 391:904-7. 1998..This mechanism augments the recombinational diversity of the primary antibody repertoire...
- A non-sequence-specific DNA binding mode of RAG1 is inhibited by RAG2Shuying Zhao
Department of Biochemistry and Molecular Biology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
J Mol Biol 387:744-58. 2009....
- Developmental separation of V(D)J recombinase expression and initiation of IgH recombination in B lineage progenitors in vivoLisa Borghesi
University of Massachusetts Medical School, Molecular Genetics and Microbiology, 55 Lake Ave North, Worcester 01655, USA
J Exp Med 199:483-9. 2004..Our results indicate that the onset of recombinase activity and the initiation of IgH recombination are developmentally distinct events in the B lineage...
- B cell progenitors are arrested in maturation but have intact VDJ recombination in the absence of Ig-alpha and Ig-betaRoberta Pelanda
Biologie III, University of Freiburg and Max Planck Institute for Immunobiology, Freiburg, Germany
J Immunol 169:865-72. 2002..Finally, Ig-alpha and Ig-beta are not redundant in their putative function, as pro-B cells from Ig-alpha and Ig-beta double-deficient mice are similar to those from single-deficient animals in every aspect analyzed...
- Commitment to the B lymphoid lineage occurs before DH-JH recombinationD Allman
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
J Exp Med 189:735-40. 1999....
- Enhancer control of V(D)J recombination at the TCRbeta locus: differential effects on DNA cleavage and joiningW M Hempel
Centre d Immunologie Institut National de la Santé et de la Recherche Médicale Centre National de la Recherche Scientifique de Marseille Luminy, Marseille Cedex 9, France
Genes Dev 12:2305-17. 1998....
- Accessibility of chromosomal recombination breaks in nuclei of wild-type and DNA-PKcs-deficient cellsDaniel Franco
Molecular and Cellular Biology Program, School of Life Sciences, Center for Infectious Diseases and Vaccinology, The Biodesign Institute at Arizona State University, BDA220, 1001 S McAllister Avenue, Tempe, AZ 85287 5501, United States
DNA Repair (Amst) 8:813-21. 2009..e., the greater the accessibility of the coding ends, the higher level of coding joints formed. Taken together, our findings provide important insights into the resolution of recombination ends by error-prone alternative NHEJ pathways...
- Lack of N regions in antigen receptor variable region genes of TdT-deficient lymphocytesT Komori
Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115
Science 261:1171-5. 1993....
- Regulation of B cell fate commitment and immunoglobulin heavy-chain gene rearrangements by IkarosDamien Reynaud
Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA
Nat Immunol 9:927-36. 2008..Thus, Ikaros is an obligate component of a network that regulates B cell fate commitment and immunoglobulin heavy-chain gene recombination...
- The mechanism of vertebrate nonhomologous DNA end joining and its role in V(D)J recombinationMichael R Lieber
USC Norris Comprehensive Cancer Ctr, Rm 5428, University of Southern California Keck School of Medicine, Department of Pathology, Los Angeles, CA 90033, USA
DNA Repair (Amst) 3:817-26. 2004..The NHEJ pathway is needed to repair these physiologic breaks, as well as challenging pathologic breaks that arise from ionizing radiation and oxidative damage to DNA...
- Identical mutations in RAG1 or RAG2 genes leading to defective V(D)J recombinase activity can cause either T-B-severe combined immune deficiency or Omenn syndromeB Corneo
Developpement Normal et Pathologique du Systeme Immunitaire, Hopital Necker Enfants Malades, Paris, France
Blood 97:2772-6. 2001..The nature of this factor is discussed...
- Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complexS D Fugmann
Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Mol Cell 5:97-107. 2000....
- The V(D)J recombinase efficiently cleaves and transposes signal jointsMatthew B Neiditch
Department of Immunology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
Mol Cell 9:871-8. 2002....
- Impaired V(D)J recombination and lymphocyte development in core RAG1-expressing miceDarryll D Dudley
Howard Hughes Medical Institute, The Children s Hospital, The Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
J Exp Med 198:1439-50. 2003..We discuss the potential implications of these findings for noncore RAG1 functions and for the ordered assembly of VH, DH, and JH segments...
- The V(D)J recombination activating gene, RAG-1D G Schatz
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142
Cell 59:1035-48. 1989..RAG-1 may activate V(D)J recombination indirectly, or it may encode the V(D)J recombinase itself...
- N-terminal RAG1 frameshift mutations in Omenn's syndrome: internal methionine usage leads to partial V(D)J recombination activity and reveals a fundamental role in vivo for the N-terminal domainsS Santagata
Ruttenberg Cancer Center, Immunobiology Center, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
Proc Natl Acad Sci U S A 97:14572-7. 2000..Taken together, our results suggest a novel mechanism for the development of immunodeficiency in a subset of Omenn's syndrome patients...
- Recombination signal sequence variations and the mechanism of patterned T-cell receptor-beta locus rearrangementAlexandru Olaru
Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, 655 West Baltimore Street, BRB 13 017, Baltimore, MD 21201, USA
Mol Immunol 40:1189-201. 2004....
- Sensing of intermediates in V(D)J recombination by ATMEric J Perkins
Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Genes Dev 16:159-64. 2002..We suggest routine surveillance of intermediates in V(D)J recombination by ATM helps suppress potentially oncogenic translocations when repair fails...
- RAG proteins shepherd double-strand breaks to a specific pathway, suppressing error-prone repair, but RAG nicking initiates homologous recombinationGregory S Lee
The Skirball Institute of Biomolecular Medicine, Lab 2 10 and Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
Cell 117:171-84. 2004..We also find that RAG-mediated nicks efficiently stimulate homologous recombination and discuss the implications of these findings for oncogenic chromosomal rearrangements, evolution, and gene targeting...
- Single-strand recombination signal sequence nicks in vivo: evidence for a capture model of synapsisJohn D Curry
Department of Molecular and Cell Biology, Division of Immunology, University of California, Berkeley, California 94720 3200, USA
Nat Immunol 6:1272-9. 2005..These data support a model of capture rather than synapsis for pairwise RSS cleavage during V(D)J recombination...
- Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavy-chain geneMartin Fuxa
Research Institute of Molecular Pathology, Vienna Biocente, A 1030 Vienna, Austria
Genes Dev 18:411-22. 2004..Pax5 subsequently activates locus contraction and distal VH-DJH rearrangements in collaboration with an unknown factor that is present in pro-B cells, but absent in thymocytes...
- Separation-of-function mutants reveal critical roles for RAG2 in both the cleavage and joining steps of V(D)J recombinationJ X Qiu
Department of Immunology, Baylor College of Medicine, Houston, TX 77030, USA
Mol Cell 7:77-87. 2001..Moreover, the discovery that some mutants block RAG-mediated hairpin opening in vitro provides a critical link between this biochemical activity and coding joint formation in vivo...
- Pax5 is required for recombination of transcribed, acetylated, 5' IgH V gene segmentsDavid G T Hesslein
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8011, USA
Genes Dev 17:37-42. 2003..The finding of transcribed, histone acetylated gene segments that fail to recombine suggests that a Pax5-dependent regulatory mechanism is required in addition to standard constraints of accessibility to control V(H) gene recombination...
- Pax5 activates immunoglobulin heavy chain V to DJ rearrangement in transgenic thymocytesLih Yun Hsu
Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
J Exp Med 199:825-30. 2004....
- A PHD finger motif in the C terminus of RAG2 modulates recombination activitySheryl K Elkin
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
J Biol Chem 280:28701-10. 2005..We propose a model in which the equilibrium between these states modulates recombination activity. Together, these data identify the PHD finger as a novel and functionally important domain of RAG2...
- Intermediates in V(D)J recombination: a stable RAG1/2 complex sequesters cleaved RSS endsJ M Jones
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 241, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 98:12926-31. 2001..Sequestration of cleaved ends within the signal end complex would account for the persistence of these ends in the cell after cleavage and may explain why they do not normally activate the DNA-damage-dependent cell cycle checkpoint...
- Identification of anti-herpes simplex virus antibody-producing B cells in a patient with an atypical RAG1 immunodeficiencyS Kumaki
Department of Pediatric Oncology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Blood 98:1464-8. 2001....
- Localized gene-specific induction of accessibility to V(D)J recombination induced by E2A and early B cell factor in nonlymphoid cellsP Goebel
The Scripps Research Institute, Department of Immunology IMM-22, La Jolla, CA 92037, USA
J Exp Med 194:645-56. 2001..We propose that accessibility occurs in a local manner, and that binding sites for factors facilitating accessibility are therefore likely to be associated with individual gene segments...
- V(D)J recombination and RAG-mediated transposition in yeastAnne E Clatworthy
Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
Mol Cell 12:489-99. 2003..Cleaved signal ends are also transposed into new sites in DNA, allowing RAG-induced transposition to be studied in vivo...
- Overlapping signals for protein degradation and nuclear localization define a role for intrinsic RAG-2 nuclear uptake in dividing cellsAshley E Ross
Department of Molecular Biology and Genetics and Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
Mol Cell Biol 23:5308-19. 2003..These results suggest that the intrinsic RAG-2 NLS functions in the nuclear uptake of RAG-2 following its reexpression in cycling cells...
- Complementation of V(D)J recombination deficiency in RAG-1(-/-) B cells reveals a requirement for novel elements in the N-terminus of RAG-1C A Roman
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Immunity 7:13-24. 1997..Our data suggest that the RAG-1 N-terminus enhances the formation of an active recombination complex that facilitates the rearrangement process...
- Access roads for RAG-ged terrains: control of T cell receptor gene rearrangement at multiple levelsFerenc Livak
Department of Microbiology and Immunology, University of Maryland School of Medicine, 655 West Baltimore Street, Baltimore, MD 21201, USA
Semin Immunol 14:297-309. 2002..The emerging results suggest that formation of the primary repertoire is controlled by two, partially independent factors: chromosomal accessibility and direct recombinase-DNA interactions...
- Functions of E2A-HEB heterodimers in T-cell development revealed by a dominant negative mutation of HEBR J Barndt
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA
Mol Cell Biol 20:6677-85. 2000..These results indicate that E2A-HEB heterodimers play obligatory roles both before and after TCRbeta gene rearrangement during the alpha/beta lineage T-cell development...
- Roles for NBS1 in alternative nonhomologous end-joining of V(D)J recombination intermediatesLudovic Deriano
Department of Pathology, The Helen L and Martin S Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine and, New York University School of Medicine, New York, NY 10016, USA
Mol Cell 34:13-25. 2009..These data demonstrate that the MRE11 complex functions at two distinct levels, regulating repair pathway choice (likely through enhancing the stability of DNA end complexes) and participating in alternative NHEJ of coding ends...
- RAG: a recombinase diversifiedAdam G W Matthews
Howard Hughes Medical Institute, Department of Biology, Cambridge, Massachusetts, USA
Nat Immunol 10:817-21. 2009..Here we discuss some of these functions and suggest that the RAG-1-RAG-2 complex nucleates a specialized subnuclear compartment that we call the 'V(D)J recombination factory'...
- Cutting edge: targeting of V beta to D beta rearrangement by RSSs can be mediated by the V(D)J recombinase in the absence of additional lymphoid-specific factorsRobert E Tillman
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110 1093, USA
J Immunol 170:5-9. 2003..These findings demonstrate that targeting of Vbeta to Dbeta rearrangement can be enforced by the V(D)J recombinase in the absence of lymphoid-specific factors other than the recombinase-activating gene-1/2 proteins...
- Restraining the V(D)J recombinaseDavid B Roth
Department of Pathology, Program in Molecular Pathogenesis, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, New York 10016, USA
Nat Rev Immunol 3:656-66. 2003..What mechanisms constrain the dangerous potential of the recombinase and preserve genomic integrity during immune-system development?..
- DNA-Rag protein interactions in the control of selective D gene utilization in the TCR beta locusAlexandru Olaru
Department of Microbiology and Immunology, Graduate Program in Molecular and Cellular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
J Immunol 171:3605-11. 2003....
- Antigen receptor loci poised for V(D)J rearrangement are broadly associated with BRG1 and flanked by peaks of histone H3 dimethylated at lysine 4Katrina B Morshead
Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 100:11577-82. 2003..The specific patterns of modification imply that the regulation of V(D)J recombination involves recruitment of specific methyltransferases in a localized manner...
- Ordered assembly of the V(D)J synaptic complex ensures accurate recombinationJessica M Jones
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5 Room 241, Bethesda, MD 20892, USA
EMBO J 21:4162-71. 2002..Additional cellular factors such as chromatin may ensure that RAG1/2 first assembles on a 12 RSS, and then a free 23 RSS enters to activate cleavage...
- In vivo transposition mediated by V(D)J recombinase in human T lymphocytesTerri L Messier
Department of Pediatrics, University of Vermont, Burlington, VT 05405, USA
EMBO J 22:1381-8. 2003..These findings implicate V(D)J recombinase-mediated transposition as a mutagenic mechanism capable of deleterious genetic rearrangements in humans...
- Targeted transposition by the V(D)J recombinaseGregory S Lee
Department of Immunology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
Mol Cell Biol 22:2068-77. 2002..The RAG proteins are capable of mediating all necessary breakage and joining events on both partner chromosomes; thus, the V(D)J recombinase may be far more culpable for oncogenic translocations than has been suspected...
- A functional analysis of the spacer of V(D)J recombination signal sequencesAlfred Ian Lee
Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut, USA
PLoS Biol 1:E1. 2003..quot; The interplay between such binary and multiplicative modes of interactions provides a general model for analyzing protein-DNA interactions in various biological systems...
- Deletion of the RAG2 C terminus leads to impaired lymphoid development in miceYoshiko Akamatsu
Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 100:1209-14. 2003..We discuss potential roles of the RAG2 C terminus in mediating rearrangement of endogenous antigen-receptor loci...
- Leaky Scid phenotype associated with defective V(D)J coding end processing in Artemis-deficient miceSean Rooney
Howard Hughes Medical Institute, The Children s Hospital, The Center for Blood Research, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
Mol Cell 10:1379-90. 2002..Finally, Artemis deficiency leads to chromosomal instability in fibroblasts, demonstrating that Artemis functions as a genomic caretaker...
- Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transpositionCatherine P Lu
Program in Molecular Pathogenesis, Department of Pathology, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Nucleic Acids Res 36:2864-73. 2008..These findings provide the first identification of components of Rag1 that are specifically required for transesterification and suggest an unexpected role for Rag2 in DNA cleavage and transposition...
- Full-length RAG-2, and not full-length RAG-1, specifically suppresses RAG-mediated transposition but not hybrid joint formation or disintegrationPatrick C Swanson
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, Nebraska 68178, USA
J Biol Chem 279:4034-44. 2004....
- The mechanism and regulation of chromosomal V(D)J recombinationCraig H Bassing
Howard Hughes Medical Institute, The Children s Hospital, The Center for Blood Research, Boston, MA 02115, USA
Cell 109:S45-55. 2002..We review our current understanding of the V(D)J recombination reaction and how it is regulated during lymphocyte development. We also discuss how defects in the mechanism or regulation of V(D)J recombination can lead to human disease...
- Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombinationChia Lun Tsai
Department of Molecular Biophysics and Biochemistry, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
Genes Dev 16:1934-49. 2002..Our results suggest that the RAG proteins have an architectural function in facilitating proper and efficient V(D)J joining, and a protective function in preventing degradation of broken ends prior to joining...
- Mutational analysis of all conserved basic amino acids in RAG-1 reveals catalytic, step arrest, and joining-deficient mutants in the V(D)J recombinaseLeslie E Huye
Department of Immunolog, Baylor College of Medicine, Houston, Texas 77030, USA
Mol Cell Biol 22:3460-73. 2002..Our analysis of these mutants provides insights into the molecular mechanisms underlying these disorders...
- V(D)J recombinationDavid G Schatz
Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520 8011, USA
Immunol Rev 200:5-11. 2004
- The phylogenetic origins of the antigen-binding receptors and somatic diversification mechanismsJohn P Cannon
Department of Molecular Genetics, All Children s Hospital, St Petersburg, FL, USA
Immunol Rev 200:12-22. 2004..Such studies have the potential for uncovering previously unknown mechanisms of generating receptor diversity...
- Genomic instability due to V(D)J recombination-associated transpositionYeturu V R Reddy
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Genes Dev 20:1575-82. 2006..In total, transposition occurred approximately once every 50,000 V(D)J recombinations. Transposition may thus contribute significantly to genomic instability...
- V(D)J recombinase mediated inter-chromosomal HPRT alterations at cryptic recombination signal sequences in peripheral human T cellsTerri L Messier
Department of Pediatrics, University of Vermont, Burlington, Vermont, USA
Hum Mutat 27:829. 2006....
- Accessibility control of V(D)J recombinationRobin Milley Cobb
Department of Microbiology and Immunology, Vanderbilt University, Nashville, Tennessee, USA
Adv Immunol 91:45-109. 2006..The discussion will focus on cis-acting regulation by ACEs, the nuclear factors that control ACE function, and the epigenetic modifications that establish recombinase accessibility...
- Amino acid residues in Rag1 crucial for DNA hairpin formationCatherine P Lu
Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, and Department of Pathology, New York University School of Medicine, New York, New York 10016, USA
Nat Struct Mol Biol 13:1010-5. 2006..Other residues surrounding the catalytic glutamate (YKEFRK) may share functional similarities with the YREK motif in IS4 family transposases...
- Essential functions of Pax5 (BSAP) in pro-B cell development: difference between fetal and adult B lymphopoiesis and reduced V-to-DJ recombination at the IgH locusS L Nutt
Research Institute of Molecular Pathology, Vienna, Austria
Genes Dev 11:476-91. 1997..In contrast, V(H)-to-D(H)J(H) rearrangements were reduced approximately 50-fold in Pax5-deficient pre-BI cells, suggesting a role for Pax5 in the developmental pathway controlling V-to-DJ recombination...
- Assessing the pathogenic potential of the V(D)J recombinase by interlocus immunoglobulin light-chain gene rearrangementS N Bailey
Department of Pathology and Graduate Program in Immunology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
Mol Cell Biol 17:887-94. 1997..Considering the large numbers of lymphocytes generated throughout life, aberrant rearrangements generated by the V(D)J recombinase may be relatively common...
- Neoteny in lymphocytes: Rag1 and Rag2 expression in germinal center B cellsS Han
Department of Microbiology and Immunology, University of Maryland School of Medicine, 655 West Baltimore Street, Baltimore, MD 21201, USA
Science 274:2094-7. 1996..Germinal center B cells thus share fundamental characteristics of immature lymphocytes, raising the possibility that antigen-dependent secondary V(D)J rearrangements modify the peripheral antibody repertoire...
- The DDE recombinases: diverse roles in acquired and innate immunityDavid H Dreyfus
Department of Pediatrics, Yale School of Medicine, New Haven, Connecticut 06511, USA
Ann Allergy Asthma Immunol 97:567-76; quiz 576-8, 602. 2006..A similar DDE-like magnesium binding site also is present in transposases, retroviral integrases, and the innate antiviral response enzymes RNAse H and RNA-induced silencing complex (RISC)...
- DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequencesC A Cuomo
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell Biol 16:5683-90. 1996..Sequence alterations that facilitate unpairing of the bases at the signal/coding border activate the cleavage reaction, suggesting that DNA distortion is critical for V(D)J recombination...
- Gene-targeted deletion and replacement mutations of the T-cell receptor beta-chain enhancer: the role of enhancer elements in controlling V(D)J recombination accessibilityJ C Bories
Unité 93, Institut National de la Sante et de la Recherche Medicale, Hopital Saint Louis, Paris, France
Proc Natl Acad Sci U S A 93:7871-6. 1996..These results demonstrate a critical role for Ebeta in promoting accessibility of the TCRbeta locus and suggest that additional negative elements may cooperate to further modulate this process...
- RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombinationAdam G W Matthews
Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
Nature 450:1106-10. 2007..Furthermore, our results provide the first evidence indicating that disrupting the read-out of histone modifications can cause an inherited human disease...
- Regulation of Tcrb recombination ordering by c-Fos-dependent RAG depositionXiaoming Wang
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Nat Immunol 9:794-801. 2008..Our results show an unexpected function for c-Fos as a direct regulator of Tcrb recombination, rather than its usual function as a transcription regulator, and provide new insight into the mechanisms of recombination ordering...
- Formation and resolution of double-strand break intermediates in V(D)J rearrangementD A Ramsden
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
Genes Dev 9:2409-20. 1995..Efficient formation of signal junctions may require cell cycle progression, or down-regulation of the recombination machinery...
- DNA cleavage of a cryptic recombination signal sequence by RAG1 and RAG2. Implications for partial V(H) gene replacementNegar S Rahman
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
J Biol Chem 281:12370-80. 2006..These results have implications for further diversification of the antigen receptor repertoire as well as the role of the RAG proteins in genomic instability...
- The bounty of RAGs: recombination signal complexes and reaction outcomesPatrick C Swanson
Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, NE 68178, USA
Immunol Rev 200:90-114. 2004..Finally, I discuss alternative DNA transactions mediated by the V(D)J recombinase, the protein-DNA complexes that support them, and factors and forces that control them...
- The taming of a transposon: V(D)J recombination and the immune systemJessica M Jones
Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington DC, USA
Immunol Rev 200:233-48. 2004..This work highlights the considerable diversity of transposition systems and their relation to V(D)J recombination...
- Analysis of the V(D)J recombination efficiency at lymphoid chromosomal translocation breakpointsS C Raghavan
Department of Pathology, Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, California 90089 9176, USA
J Biol Chem 276:29126-33. 2001..These results quantify one of the three major factors that determine the frequency of these translocations in T-cell acute lymphocytic leukemia...
- Regulation of T-cell receptor beta-chain gene assembly by recombination signals: the beyond 12/23 restrictionRobert E Tillman
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110 1093, USA
Immunol Rev 200:36-43. 2004..By preserving Dbeta gene segment utilization, the B12/23 restriction is required, at a minimum, for the generation of a diverse repertoire of TCRbeta chains...
- New concepts in the regulation of an ancient reaction: transposition by RAG1/RAG2Monalisa Chatterji
Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA
Immunol Rev 200:261-71. 2004..A major challenge has been to determine how the transposition activity of the RAG proteins is regulated in vivo. Although a variety of mechanisms have been suggested by recent studies, a clear resolution of this issue remains elusive...
- Joining mutants of RAG1 and RAG2 that demonstrate impaired interactions with the coding-end DNAFumikiyo Nagawa
Department of Biophysics and Biochemistry, Graduate School of Science, and Core Research for Evolutional Science and Technology Program of the Japan Science and Technology Agency, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
J Biol Chem 279:38360-8. 2004..A possible role of this RAG coding region interaction is discussed in the context of V(D)J recombination...
- DNA cleavage activity of the V(D)J recombination protein RAG1 is autoregulatedPallabi De
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
Mol Cell Biol 24:6850-60. 2004..We propose how the combined actions of the RAG1 domains may function in V(D)J recombination and also in aberrant cleavage reactions that may lead to genomic instability in B and T lymphocytes...
- Deletion of germline promoter PD beta 1 from the TCR beta locus causes hypermethylation that impairs D beta 1 recombination by multiple mechanismsC E Whitehurst
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Immunity 13:703-14. 2000..These findings suggest that methylation can regulate V(D)J recombination both at a general level by influencing regional chromatin accessibility and specifically by blocking RSS recognition or cleavage by the V(D)J recombinase...
- A direct interaction between the RAG2 C terminus and the core histones is required for efficient V(D)J recombinationKelly L West
Immunobiology Center, Mount Sinai School of Medicine, New York, New York 10029, USA
Immunity 23:203-12. 2005..Point mutations that disrupt the RAG2-histone association inhibit the efficiency of the V(D)J recombination reaction at the endogenous immunoglobulin locus, with the most dramatic effect in the V to DJ(H) rearrangement...
- Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinaseD R Kim
Molecular Biology Department, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
Genes Dev 13:3070-80. 1999..Our results support a model in which the RAG1 protein contains the active site of the V(D)J recombinase and are interpreted in light of predictions about the structure of RAG1...
- Mutational analysis of RAG1 and RAG2 identifies three catalytic amino acids in RAG1 critical for both cleavage steps of V(D)J recombinationM A Landree
Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Genes Dev 13:3059-69. 1999..These data suggest that RAG-mediated DNA cleavage involves coordination of divalent metal ion(s) by RAG1...
- Turning T-cell receptor beta recombination on and off: more questions than answersAnnette M Jackson
Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA
Immunol Rev 209:129-41. 2006..We highlight the many unresolved issues and we discuss how recent concepts emerging from studies of other antigen receptor loci may (or may not) help to resolve these issues...
- Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repairQi Ding
College of Veterinary Medicine and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan 48824, USA
Mol Cell Biol 23:5836-48. 2003..However, autophosphorylation at these sites may not be sufficient for kinase disassembly...
- Alternative pathways for the repair of RAG-induced DNA breaksDavid M Weinstock
Department of Medicine, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
Mol Cell Biol 26:131-9. 2006..Thus, RAG-generated DSBs are typically repaired by the NHEJ pathway in ES cells, but in the absence of NHEJ components, a substantial fraction of breaks can be efficiently channeled into alternative pathways in these cells...
- Biochemistry of V(D)J recombinationD G Schatz
Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520 8011, USA
Curr Top Microbiol Immunol 290:49-85. 2005..We discuss how the RAG proteins interact with DNA and how coordinate cleavage of the DNA at two sites might be achieved. Finally, we consider the RAG proteins and V(D)J recombination from an evolutionary point of view...
- The ontogeny and fate of NK cells marked by permanent DNA rearrangementsKristy Pilbeam
Department of Immunology, University of Pittsburgh School of Medicine, 200 Lothrop Street, Pittsburgh, PA 15261, USA
J Immunol 180:1432-41. 2008....
- Induction of V(D)J-mediated recombination of an extrachromosomal substrate following exposure to DNA-damaging agentsRobert L Pinsonneault
Department of Pediatrics, University of Vermont, Burlington, Vermont, USA
Environ Mol Mutagen 48:440-50. 2007....
- V(D)J recombination: how to tame a transposaseVicky L Brandt
Program in Molecular Pathogenesis, The Skirball Institute, New York University School of Medicine, New York, NY, USA
Immunol Rev 200:249-60. 2004..These novel strategies for harnessing the RAG transposase not only shed light on V(D)J recombination but also may provide insight into the regulation of other transposases...
- V(D)J recombination and the evolution of the adaptive immune systemEleonora Market
Rockefeller University, New York, New York, USA
PLoS Biol 1:E16. 2003
- Quantitative analyses of RAG-RSS interactions and conformations revealed by atomic force microscopyJeffrey W Pavlicek
School of Life Sciences, Center for Infectious Disease and Vaccinology, The Biodesign Institute, Arizona State University, Tempe, Arizona 85287 5501, USA
Biochemistry 47:11204-11. 2008....
- Does artemis end the hunt for the hairpin-opening activity in V(D)J recombination?Mark S Schlissel
Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Cell 109:1-4. 2002..Both genetic and biochemical data point toward a physiological role for this complex as the elusive hairpin-opening activity in V(D)J recombination...
- V(D)J recombination is not required for the development of lymphoma in p53-deficient miceM Nacht
Howard Hughes Medical Institute, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
Cell Growth Differ 9:131-8. 1998..These data suggest that normal V(D)J recombination is not required for the development of thymic lymphoma in the context of a p53 germ-line mutation...
- RAG-1 and RAG-2 gene expression and V(D)J recombinase activity are enhanced by protein phosphatase 1 and 2A inhibition in lymphocyte cell linesA M Casillas
Department of Biology, University of California at Los Angeles 90024
Mol Immunol 32:167-75. 1995..These results suggest novel intracellular mechanisms for the upregulation of RAG gene expression and confirm a role for type 1 and 2A phosphatases in the control of RAG gene expression and recombinase activity in lymphocyte cell lines...
- Control of chromatin accessibility for V(D)J recombination by interleukin-7J Huang
Laboratory of Molecular Immuneregulation, SAIC-FCRDC, National Cancer Institute, Frederick, Maryland, USA
J Leukoc Biol 69:907-11. 2001..This opening leads to a higher accessibility for the transcription machinery and increased accessibility of the Rag heterodimer that performs the cleavage of DNA...
- Functional organization of single and paired V(D)J cleavage complexesM A Landree
Interdepartmental Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Mol Cell Biol 21:4256-64. 2001..These results strongly suggest that two RAG-1 dimers are responsible for RSS cleavage in a synaptic complex, with one monomer of each dimer catalyzing both nicking and hairpin formation at each RSS...
- DNA repair: spot(light)s on chromatinM Modesti
Department of Cell Biology and Genetics, Erasmus University Rotterdam, PO Box 1738, 3000 DR, Rotterdam, The Netherlands
Curr Biol 11:R229-32. 2001..Recent studies on the phosphorylation of a histone 2A variant have revealed that this chromatin modification is a general and evolutionarily conserved cellular response to DNA double-strand breaks...
- The RAG proteins in V(D)J recombination: more than just a nucleaseM J Sadofsky
Medical College of Georgia, Institute of Molecular Medicine and Genetics, CB 2803, Augusta, GA 30912, USA
Nucleic Acids Res 29:1399-409. 2001..Two proteins, RAG1 and RAG2, together form the nuclease that cleaves the DNA at the border of the signal sequences. Additional roles of these proteins in organizing the reaction complex for subsequent steps are explored...
- Regulation of the VDJ recombinase during genotoxic stressKarla K Rodgers; Fiscal Year: 2013..The ability to enhance such a regulatory process may lead to decreased risks of developing lymphoid malignancies following exposure to radiation and other DNA damaging drugs. ..
- Long-Range Genetic and Epigenetic Control of Igh Gene AssemblyEugene M Oltz; Fiscal Year: 2010..We propose studies to address how precursor B cells control long-range changes in the spatial organization of the immunoglobulin heavy chain locus to ensure its proper assembly and pursuant generation of an adaptive immune system. ..
- LOCALIZED REVISION OF EPIGENETIC LANDSCAPES INDUCED BY DNA DOUBLE-STRAND BREAKSBarry P Sleckman; Fiscal Year: 2012..We now propose to identify histone modifications that function as intermediates to coordinate the normal repair of DNA-DSBs in mammalian cells. ..
- Accessibility Control of Antigen Receptor Gene AssemblyEugene M Oltz; Fiscal Year: 2013..We propose studies to address how precursor T lymphocytes activate the T cell receptor beta locus at the earliest stages of thymocyte development to ensure its proper assembly by V(D)J recombination. ..
- Long-Range Genetic and Epigenetic Control of Tcrb Gene AssemblyEugene M Oltz; Fiscal Year: 2010..We propose studies to address how precursor T lymphocytes control long-range changes in the spatial organization of T cell receptor beta loci to ensure their proper assembly and pursuant generation of a normal adaptive immune system. ..
- Spatiotemporal control of recombination by the RAG proteins and ATMJANE AMANDA SKOK; Fiscal Year: 2010..We propose to further these studies to understand precisely how these factors achieve these unexpected effects. ..
- BIOCHEMISTRY AND REGULATION OF V(D)J RECOMBINATIONMark S Schlissel; Fiscal Year: 2012..Our approach is to take what has been learned in vitro and use it to frame and test hypotheses regarding the regulation of recombination in vivo. ..
- NORMAL AND MUTANT LYMPHOID V(D)J RECOMBINASEMichael R Lieber; Fiscal Year: 2011..In the long-term, all of these studies will help establish optimal therapy for many human SCID patients and to understand the human immune repertoire. ..
- Temporal and Spatial Control of V(D)J RecombinationSTEPHEN V DESIDERIO; Fiscal Year: 2013....
- Optimal Generation and Characterization of iPS Cell Lines from Healthy and ADHD SKwang Soo Kim; Fiscal Year: 2012..This approach will advance the iPS cell technology and provide a platform for investigating the biological and molecular mechanisms underlying ADHD pathogenesis. ..
- Optimal Generation and Characterization of iPS Cell Lines from Healthy and ADHD SKwang Soo Kim; Fiscal Year: 2010..This approach will advance the iPS cell technology and provide a platform for investigating the biological and molecular mechanisms underlying ADHD pathogenesis. ..
- Molecular basis of the requirements for IRF4 and IRF8 in pre-B cell developmentRunqing Lu; Fiscal Year: 2010..In the absence of IRF4,8, pro-B cell development appears to be normal but pre-B cell development is blocked. We hypothesize that expression of IRF4,8 is induced at the pre-B stage by pre-BCR though Blnk/Btk-mediated signaling. ..
- EXPRESSION OF IMMUNOGLOBULIN GENES IN TRANSGENIC MICEUrsula Storb; Fiscal Year: 2000..It is expected that these experiments will contribute to the understanding of the control of B cell development and Ig gene expression, and will provide new insights into immunological disorders with a B cell component. ..
- CONTROL OF IMMUNOGLOBULIN SYNTHESISUrsula Storb; Fiscal Year: 2001..Understanding this mechanism will provide a fundamental basis for the unraveling of many diseases involving the immune system, such as autoimmunities, infectious diseases, allergies and cancer. ..
- SOMATIC DNA RECOMBINATION IN ANTIBODY GENESHitoshi Sakano; Fiscal Year: 1993..They will also shed light on the basic processes of some immunodeficiencies and chromosome abnormalities caused by DNA arrangement...
- MOLECULAR CONTROL OF ANTIGEN RECEPTOR GENE ASSEMBLYEugene Oltz; Fiscal Year: 2000..The interactive environment, and diverse expertise offered by Dr. Oltz's colleagues at Vanderbilt, and its Cancer Center, provide an ideal scientific setting to foster these broadened approaches. ..
- PROTEOMEWORKSTM SYSTEMRichard Cook; Fiscal Year: 2001..For all of these projects, the requested 2-D gel/MALDI-TOF ProteomeWorks(TM) System is essential for accomplishing the stated goals. ..
- ASSEMBLY AND EXPRESSION OF IG VARIABLE REGION GENESFrederick Alt; Fiscal Year: 2000....
- ORGANIZATION AND EXPRESSION OF V GENESPETER BRODEUR; Fiscal Year: 2007..We will use a novel cell line model as well as primary bone marrow cells to determine the mechanism through which IL-7 receptor signaling selectively mediates D-distal (5') Vh gene accessibility. ..
- SCID MICE AND THE CONTROL OF LYMPHOCYTE DIFFERENTIATIONMELVIN BOSMA; Fiscal Year: 1993..The experiments outlined in this proposal should tell us more about the effect of SCID on early lymphocyte development. This is important for understanding the genetic control of both normal and abnormal lymphocyte differentiation...
- MOLECULAR CONTROL OF ANTIGEN RECEPTOR GENE ASSEMBLYEugene Oltz; Fiscal Year: 2000..Collectively, these studies will yield fundamental insights into the mechanisms that direct, the re-direct recombinase activity to specific antigen receptor loci, and thereby ensure proper lymphocyte development. ..
- Protein-DNA Interactions in V(D)J RecombinationKarla Rodgers; Fiscal Year: 2007..Results from these studies will provide a valuable framework in the determination of the assembly and mechanism of the V(D)J recombinase. ..
- DIMINISHED V(D)J RECOMBINATION DURING B CELL DEVELOPMENTRachel Gerstein; Fiscal Year: 2001..This work uses new techniques to study the effect of aging on the immune system and intends, as a long term goal, to identify mechanisms responsible for diminished B cell production in aged mice and humans. ..
- New models for RAG-mediated lymphomagenesisSANDRA SALUS; Fiscal Year: 2004....
- Elements of B Cell MemoryMarilia Cascalho; Fiscal Year: 2005..memory cell upon engagement by antigen. These goals will be achieved by analysis of the antigen specific Ig titer and by analysis of somatic hypermutation of the heavy-chain V exons following immunization with the cognate antigen. ..
- MOLECULAR CONTROL OF ANTIGEN RECEPTOR GENE ASSEMBLYEugene Oltz; Fiscal Year: 2002..studies will resolve a fundamental question in immunology; how do developing lymphocytes regulate choices amongst large arrays of gene segments, any of which could serve as sequence-specific targets for the V(D)J recombinase complex? ..
- Control of B Cell Development by Histone MethylationLANCE THOMAS; Fiscal Year: 2006..These experiments will reveal the role of G9a in the regulation of B cell maturation, antigen receptor gene assembly, and effector functions in mature B cells. [unreadable] [unreadable] [unreadable]..
- V(D)J RECOMBINASE & CHROMOSOME TRANSLOCATION IN LYMPHOMAK VANASSE; Fiscal Year: 2003..Such insight may further the understanding of lymphomagenesis and may help in the development of novel anti-tumor strategies. ..
- PESTICIDES AND VDJ-RECOMBINASE MUTATIONS IN CHILD NHLJonathan Buckley; Fiscal Year: 2002..The investigators state that the results will be potentially relevant to childhood leukemia as well as lymphoma, and may also have implications for these malignancies in adults. ..
- Lymphomagenesis as a Response to Antigenic StimulationSITING STEPHEN CHEN; Fiscal Year: 2002..These studies may improve the understanding of how perturbations in immunity may contribute to NHL. ..
- IN VIVO SOMATIC MUTATIONS IN CHILDREN WITH CANCERBARRY FINETTE; Fiscal Year: 2001..In addition, the isolation of mutator phenotype clones will allow for future m e chanistic studies characterizing the genetic and biologic defect(s) associated with leukemogenesis in children. ..
- IMMUNOGLOBULIN AND T CELL RECEPTOR GENE ASSEMBLYDavid Schatz; Fiscal Year: 2006..Overall, our experiments will address both the targeting and rejoining functions of the RAG proteins, with the long term goal of linking these fundamental activities to chromosomal translocations found in human malignancies. ..
- Targeting of somatic hypermutation and gene conversionDavid Schatz; Fiscal Year: 2006..The results of these experiments should set the stage for significant progress toward an understanding of the mechanism by which protein factors and DNA sequences collaborate to target SM and GC to Ig genes. ..
- Characterization of V(D)J cleavage and repair complexesPATRICK SWANSON; Fiscal Year: 2007..abstract_text> ..
- Transcriptional Control of AICDA ExpressionFerenc Livak; Fiscal Year: 2005..abstract_text> ..
- BIOCHEMISTRY AND REGULATION OF V (D) J RECOMBINATIONMark Schlissel; Fiscal Year: 2004....
- Control Of Tcr Data And Tcr Alpha RearrangementMICHAEL KRANGEL; Fiscal Year: 2005..abstract_text> ..
- HUMAN V(D)J RECOMBINASE IN NEOPLASTIC AND PRIMARY CELLSMichael Lieber; Fiscal Year: 2004..Corresponding studies of such regions on minichromosomes will permit the testing of the effects of the direction of DNA replication and transcription. ..
- c-Abl and PKC-eta in Cell Development and LeukemiaMark Schlissel; Fiscal Year: 2008..abstract_text> ..
- SOMATIC MUTATIONS IN CHILDRENBARRY FINETTE; Fiscal Year: 2002..Results obtained from these studies will provide fundamental insights regarding the clinical relevance of age-specific, spontaneous and environmentally induced somatic mutations in children. ..
- REGULATION OF V(D)J RECOMBINATION IN B CELL DEVELOPMENTRachel M Gerstein; Fiscal Year: 2011..abstract_text> ..
- Notch signaling in B cell development and survivalWarren Pear; Fiscal Year: 2002..abstract_text> ..
- MDS Nordian Gammacell-40 Exactor Whole Animal IrradiatorJohn Monroe; Fiscal Year: 2002..abstract_text> ..
- MOLECULAR ANALYSIS OF THE ANTIH INFLUENZAE RESPONSEANN FEENEY; Fiscal Year: 2001..We will also further analyze the diversity in the ani-hib response through the use of combinatorial libraries, correlating affinity with the Vh, Vk and CDR3's which are used. ..
- GENETIC PATHWAY TO IGH TRANSLOCATIONS IN LYMPHOMADENNIS WILLERFORD; Fiscal Year: 2003..Ultimately, strategies to prevent lymphoma development in groups at increased risk may emerge. ..
- Role of the BCR in B lymphocyte survival and developmentJohn Monroe; Fiscal Year: 2002....
- Intrinsic and extrinsic control of B cells toleranceJohn Monroe; Fiscal Year: 2004..abstract_text> ..
- Analyzing VDJ recombination at the single molecule levelDavid Roth; Fiscal Year: 2005....
- Control of Accessibility for V(D) J RecombinationANN FEENEY; Fiscal Year: 2006..We will determine if other transcription factors such as Pax5 or Btk will induce rearrangement of other Ig loci, such as Vh or lambda. ..
- Modulatory signaling motifs in B cell antigen receptor functionRoberta Pelanda; Fiscal Year: 2006..These studies will provide new avenues with which to ameliorate immunodeficiencies, autoimmunities and cancer. ..
- Genotoxicity & Emergence of Genome Instability in HumansBARRY FINETTE; Fiscal Year: 2007....
- The role of AID protein in the host response to viral infectionF Papavasiliou; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Tolerance in polyclonal and oligoclonal immune systemsRoberta Pelanda; Fiscal Year: 2007..abstract_text> ..
- Receptor Editing in Autoimmune and Normal MiceANN FEENEY; Fiscal Year: 2009..The information gained in these aims should give insights into the response of the various Ig loci to receptor editing signals, and how these processes may potentially be misregulated in B cells of autoimmune mice. ..
- B CELL REPERTOIRE FORMATIONANN FEENEY; Fiscal Year: 2009..Together, these studies should give insight into the epigenetic mechanisms and cis-elements which control VH gene rearrangement in fetal and adult pro-B cells. ..