subtilisin

Summary

Summary: A serine endopeptidase isolated from Bacillus subtilis. It hydrolyzes proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. It also hydrolyzes peptide amides. (From Enzyme Nomenclature, 1992) EC 3.4.21.62.

Top Publications

  1. ncbi Cation-dependent stability of subtilisin
    P A Alexander
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 40:10634-9. 2001
  2. ncbi Engineering a substrate-specific cold-adapted subtilisin
    Nikolaj Tindbaek
    Molecular Biotechnology, Novozymes A S, Krogshoejvej 36, DK 2880 Bagsvaerd, Denmark
    Protein Eng Des Sel 17:149-56. 2004
  3. ncbi Binding of proprotein convertase subtilisin/kexin type 9 to epidermal growth factor-like repeat A of low density lipoprotein receptor decreases receptor recycling and increases degradation
    Da Wei Zhang
    Department of Molecular Genetics, The Donald W Reynolds Cardiovascular Clinical Research Center, Howard Hughes Institute, University of Texas Southwestern Medical Center, Dallas 75390, USA
    J Biol Chem 282:18602-12. 2007
  4. pmc The subtilisin-like protease AprV2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates
    Ruth M Kennan
    Monash University, Clayton, Victoria, Australia
    PLoS Pathog 6:e1001210. 2010
  5. ncbi Identification of subtilisin, Epr and Vpr as enzymes that produce CSF, an extracellular signalling peptide of Bacillus subtilis
    Sara Lanigan-Gerdes
    Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095, USA
    Mol Microbiol 65:1321-33. 2007
  6. pmc A subtilisin-like protein from soybean contains an embedded, cryptic signal that activates defense-related genes
    Gregory Pearce
    Institute of Biological Chemistry, Washington State University, Pullman, WA 99164 6340, USA
    Proc Natl Acad Sci U S A 107:14921-5. 2010
  7. ncbi The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-like serine proteases
    G D Brown
    Department of Medical Biochemistry MRC Centre for Molecular and Cellular Biology, University of Stellenbosch, 7505, Tygerberg, South Africa
    Gene 254:147-55. 2000
  8. ncbi Atorvastatin increases human serum levels of proprotein convertase subtilisin/kexin type 9
    Holly E Careskey
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    J Lipid Res 49:394-8. 2008
  9. pmc A plant alternative to animal caspases: subtilisin-like proteases
    A B Vartapetian
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow, Russia
    Cell Death Differ 18:1289-97. 2011
  10. pmc Microneme protein 5 regulates the activity of Toxoplasma subtilisin 1 by mimicking a subtilisin prodomain
    Savvas Saouros
    Division of Molecular Biosciences, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
    J Biol Chem 287:36029-40. 2012

Detail Information

Publications203 found, 100 shown here

  1. ncbi Cation-dependent stability of subtilisin
    P A Alexander
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 40:10634-9. 2001
    b>Subtilisin BPN' contains two cation binding sites. One specifically binds calcium (site A), and the other can bind both divalent and monovalvent metals (site B)...
  2. ncbi Engineering a substrate-specific cold-adapted subtilisin
    Nikolaj Tindbaek
    Molecular Biotechnology, Novozymes A S, Krogshoejvej 36, DK 2880 Bagsvaerd, Denmark
    Protein Eng Des Sel 17:149-56. 2004
    One region predicted to be highly flexible for a psychrophilic enzyme, TA39 subtilisin (S39), was transferred in silico to the mesophilic subtilisin, savinase (EC 3.4.21.62), from Bacillus lentus (clausii)...
  3. ncbi Binding of proprotein convertase subtilisin/kexin type 9 to epidermal growth factor-like repeat A of low density lipoprotein receptor decreases receptor recycling and increases degradation
    Da Wei Zhang
    Department of Molecular Genetics, The Donald W Reynolds Cardiovascular Clinical Research Center, Howard Hughes Institute, University of Texas Southwestern Medical Center, Dallas 75390, USA
    J Biol Chem 282:18602-12. 2007
    Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes degradation of hepatic low density lipoprotein receptors (LDLR), the major route of clearance of circulating cholesterol...
  4. pmc The subtilisin-like protease AprV2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates
    Ruth M Kennan
    Monash University, Clayton, Victoria, Australia
    PLoS Pathog 6:e1001210. 2010
    ..D. nodosus secretes three subtilisin-like proteases whose analysis forms the basis of diagnostic tests that differentiate between virulent and benign ..
  5. ncbi Identification of subtilisin, Epr and Vpr as enzymes that produce CSF, an extracellular signalling peptide of Bacillus subtilis
    Sara Lanigan-Gerdes
    Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095, USA
    Mol Microbiol 65:1321-33. 2007
    ..Mutants lacking the three proteases that fit these criteria, subtilisin, Epr and Vpr, had a defect in CSF production...
  6. pmc A subtilisin-like protein from soybean contains an embedded, cryptic signal that activates defense-related genes
    Gregory Pearce
    Institute of Biological Chemistry, Washington State University, Pullman, WA 99164 6340, USA
    Proc Natl Acad Sci U S A 107:14921-5. 2010
    ..The amino acid sequence was determined and was found to be derived from a member of the subtilisin-like protease (subtilase) family...
  7. ncbi The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-like serine proteases
    G D Brown
    Department of Medical Biochemistry MRC Centre for Molecular and Cellular Biology, University of Stellenbosch, 7505, Tygerberg, South Africa
    Gene 254:147-55. 2000
    ..contains a catalytic triad (Asp, His, Ser) within highly conserved sequences, typical of proteases of the subtilisin family. These genes are also present in M...
  8. ncbi Atorvastatin increases human serum levels of proprotein convertase subtilisin/kexin type 9
    Holly E Careskey
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    J Lipid Res 49:394-8. 2008
    Proprotein convertase subtilisin/kexin type 9 (PCSK9) has gained attention as a key regulator of serum low density lipoprotein cholesterol (LDL-C) levels...
  9. pmc A plant alternative to animal caspases: subtilisin-like proteases
    A B Vartapetian
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow, Russia
    Cell Death Differ 18:1289-97. 2011
    ..Here, we review recent data showing that some caspase-like activities are attributable to the plant subtilisin-like proteases, saspases and phytaspases...
  10. pmc Microneme protein 5 regulates the activity of Toxoplasma subtilisin 1 by mimicking a subtilisin prodomain
    Savvas Saouros
    Division of Molecular Biosciences, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
    J Biol Chem 287:36029-40. 2012
    ..The subtilisin protease TgSUB1 trims several MICs on the parasite surface to activate gliding motility and host invasion...
  11. ncbi Identification and characterization of a surface-associated, subtilisin-like serine protease in Trichomonas vaginalis
    Pablo Hernández-Romano
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Tercer Circuito Exterior, Ciudad Universitaria, 04510 Mexico DF, Mexico
    Parasitology 137:1621-35. 2010
    ..In this report we identify and characterize a putative subtilisin-like serine protease (SUB1). The sub1 gene encodes a 101-kDa protein...
  12. ncbi Discovery of a new role of human resistin in hepatocyte low-density lipoprotein receptor suppression mediated in part by proprotein convertase subtilisin/kexin type 9
    Michelle Melone
    Department of Medicine, Thrombosis and Atherosclerosis Research Institute, David Braley Cardiac, Vascular and Stroke Research Institute, McMaster University, Hamilton, Ontario, Canada
    J Am Coll Cardiol 59:1697-705. 2012
    ..In this study, our goal was to determine if human resistin plays a role in regulating the uptake of atherogenic low-density lipoproteins in human hepatocytes...
  13. ncbi Decontamination of prion protein (BSE301V) using a genetically engineered protease
    J Dickinson
    Health Protection Agency, Porton Down, Salisbury, UK
    J Hosp Infect 72:65-70. 2009
    ..Here we explored the use of MC3, a genetically engineered variant of Bacillus lentus subtilisin. MC3 was used to digest BSE301V infectious mouse brain homogenate (iMBH)...
  14. ncbi Crystal structure of unautoprocessed precursor of subtilisin from a hyperthermophilic archaeon: evidence for Ca2+-induced folding
    Shun ichi Tanaka
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 282:8246-55. 2007
    The crystal structure of an active site mutant of pro-Tk-subtilisin (pro-S324A) from the hyperthermophilic archaeon Thermococcus kodakaraensis was determined at 2.3 A resolution...
  15. pmc Improving the pathogenicity of a nematode-trapping fungus by genetic engineering of a subtilisin with nematotoxic activity
    Johan Ahman
    Department of Microbial Ecology, Ecology Building, Lund University, S 223 62 Lund, Sweden
    Appl Environ Microbiol 68:3408-15. 2002
    ..In this paper we report that a subtilisin-like extracellular serine protease designated PII is an important pathogenicity factor in the common nematode-..
  16. ncbi Protein engineering of subtilisin
    P N Bryan
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, 20850, Rockville, MD, USA
    Biochim Biophys Acta 1543:203-222. 2000
    The serine protease subtilisin is an important industrial enzyme as well as a model for understanding the enormous rate enhancements affected by enzymes...
  17. pmc The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway
    Marc A B Kolkman
    Genencor, a Danisco Division, Archimedesweg 30, 2333 CN Leiden, The Netherlands
    Appl Environ Microbiol 74:7507-13. 2008
    ..subtilis Tat substrates YwbN and PhoD. Remarkably, the YwbN signal peptide directed secretion of active subtilisin, a typical Sec substrate, via the B. subtilis TatAyCy route...
  18. pmc The cell envelope subtilisin-like proteinase is a virulence determinant for Streptococcus suis
    Laetitia Bonifait
    Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Universite Laval, Quebec City, Quebec, Canada
    BMC Microbiol 10:42. 2010
    ..suis mutant library created by the insertion of Tn917 transposon in order to isolate a mutant deficient in a cell surface proteinase. We characterized the gene and assessed the proteinase for its potential as a virulence factor...
  19. ncbi Preparation and characterization of cross-linked enzyme aggregates (CLEA) of subtilisin for controlled release applications
    K Sangeetha
    Chemical Science and Technology Division, Regional Research Laboratory CSIR, Trivandrum 695019, India
    Int J Biol Macromol 43:314-9. 2008
    Enzyme stabilization is one of the major challenges in the biocatalytic process optimization. Subtilisin was aggregated using ammonium sulphate and polyethylene glycol with surfactants like triton X-100 and tween 20...
  20. ncbi Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation
    Shun ichi Tanaka
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka, Japan
    J Mol Biol 372:1055-69. 2007
    b>Subtilisin from the hyperthermophilic archaeon Thermococcus kodakaraensis (Tk-subtilisin) is matured from Pro-Tk-subtilisin upon autoprocessing and degradation of the propeptide...
  21. ncbi Feasibility of infectious prion digestion using mild conditions and commercial subtilisin
    John L Pilon
    National Wildlife Research Center, 4101 LaPorte Ave, Fort Collins, CO 80521, USA
    J Virol Methods 161:168-72. 2009
    Two serine protease enzymes, subtilisin 309 and subtilisin 309-v, were used to digest brain homogenates containing high levels of prion infectivity using mildly alkaline conditions to investigate prion decontamination methods...
  22. ncbi Purification and characterization of a fibrinolytic subtilisin-like protease of Bacillus subtilis TP-6 from an Indonesian fermented soybean, Tempeh
    Seong Bo Kim
    Department of Biotechnology, Yonsei University, 120 749, Seoul, South Korea
    J Ind Microbiol Biotechnol 33:436-44. 2006
    ..The result demonstrates that TPase is a serine protease of the subtilisin family consisting of 275 amino acid residues in its mature form...
  23. ncbi Characterization of a subtilisin-like protease with apical localization from microsporidian Nosema bombycis
    Xiaoqun Dang
    The State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest University, Beibei, Chongqing 400716, People s Republic of China
    J Invertebr Pathol 112:166-74. 2013
    ..Here we identified a subtilisin-like serine protease NbSLP1 which contains an inhibitor_I9 and a peptidase_S8 domain...
  24. ncbi Immobilization of subtilisin on polycaprolactam for antimicrobial food packaging applications
    Prabhawathi Veluchamy
    Department of Biotechnology, Indian Institute of Technology Madras, Adyar, Chennai 600 036, India
    J Agric Food Chem 59:10869-78. 2011
    b>Subtilisin was immobilized on polycaprolactam and used for food packaging applications to reduce the transference of microorganisms from the packaging material to the packaged food material...
  25. ncbi Molecular and structural characterization of a surfactant-stable high-alkaline protease AprB with a novel structural feature unique to subtilisin family
    Aihua Deng
    Department of Industrial Microbiology and Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Biochimie 93:783-91. 2011
    ..In contrast with members of subtilisin family, such as M-protease and subtilisin BPN', AprB harbored a high content of Glu and Asp residues, and a low ..
  26. ncbi An extremely thermotolerant, alkaliphilic subtilisin-like protease from hyperthermophilic Bacillus sp. MLA64
    Milad Lagzian
    Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
    Int J Biol Macromol 51:960-7. 2012
    ..The protease was inhibited by PMSF but not by TPCK and TLCK, suggesting that it can be a subtilisin-like protease...
  27. ncbi Molecular and immunological characterization of subtilisin like serine protease, a major allergen of Curvularia lunata
    Prabhanshu Tripathi
    Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India
    Immunobiology 216:402-8. 2011
    ..cDNA library screening identified partial protease clones. A clone showed significant homology to subtilisin like serine proteases from Aspergillus and Penicillium species...
  28. ncbi Crystal structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo L
    Kazutaka Murayama
    Division of Biomedical Measurements and Diagnostics, Graduate School of Biomedical Engineering, Tohoku University, Sendai 980 8575, Japan
    J Mol Biol 423:386-96. 2012
    ..A structural homology search indicated that the catalytic domain of cucumisin shares structural similarity with subtilisin and subtilisin-like fold enzymes...
  29. pmc Structural basis for dual-inhibition mechanism of a non-classical Kazal-type serine protease inhibitor from horseshoe crab in complex with subtilisin
    Rajesh T Shenoy
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 6:e18838. 2011
    ..The CrSPIs were biochemically active, especially CrSPI-1, which potently inhibited subtilisin (Ki = 1.43 nM)...
  30. ncbi Highly efficient gene expression of a fibrinolytic enzyme (subtilisin DFE) in Bacillus subtilis mediated by the promoter of alpha-amylase gene from Bacillus amyloliquefaciens
    Lu Xiao
    College of Life Sciences, Sichuan University, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, P R China
    Biotechnol Lett 26:1365-9. 2004
    b>Subtilisin DFE is a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4. The promoter and signal peptide-coding sequence of alpha-amylase gene from B...
  31. pmc Regulation of an intracellular subtilisin protease activity by a short propeptide sequence through an original combined dual mechanism
    Michael Gamble
    School of Biosciences, Cardiff University, Cardiff CF10 3AT, United Kingdom
    Proc Natl Acad Sci U S A 108:3536-41. 2011
    A distinct class of the biologically important subtilisin family of serine proteases functions exclusively within the cell and forms a major component of the bacilli degradome...
  32. ncbi [Occupational airway sensitization due to subtilisin]
    V van Kampen
    Berufsgenossenschaftliches Forschungsinstitut für Arbeitsmedizin, BGFA, Bochum, Germany
    Pneumologie 56:182-6. 2002
    ..b>Subtilisin, a frequently used enzyme often has been shown to cause airway sensitization...
  33. ncbi Crystal structure of Tk-subtilisin folded without propeptide: requirement of propeptide for acceleration of folding
    Shun ichi Tanaka
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    FEBS Lett 582:3875-8. 2008
    Tk-subtilisin (a subtilisin homologue from Thermococcus kodakaraensis) is matured from Pro-Tk-subtilisin upon autoprocessing and degradation of Tk-propeptide...
  34. ncbi Directed evolution of Tk-subtilisin from a hyperthermophilic archaeon: identification of a single amino acid substitution responsible for low-temperature adaptation
    M A Pulido
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Protein Eng Des Sel 20:143-53. 2007
    Tk-subtilisin from the hyperthermophilic archaeon Thermococcus kodakaraensis is synthesized in a prepro-form (prepro-Tk-subtilisin), secreted in a pro-form (pro-Tk-subtilisin), and matured to an active form (mat-Tk-subtilisin*; a Ca(2+)-..
  35. ncbi A near real-time system for continuously monitoring airborne subtilisin-type enzymes in the industrial atmosphere
    Frederick J Rowell
    North East Biotechnology Centre, School of Health, Natural and Social Sciences, University of Sunderland, Sunderland, SR1 3SD, UK
    J Environ Monit 9:33-43. 2007
    ..The system is able to monitor airborne concentrations of subtilisin-type enzymes in the workplace atmosphere on a continuous basis...
  36. ncbi Mutational analysis of the autoprocessing site of subtilisin YaB-G124A
    Yuh Shin Chang
    Institute of Biochemistry, National Yang Ming University, Taipei, Taiwan, Republic of China
    Biochem Biophys Res Commun 291:165-9. 2002
    ..was evaluated from hydrolysis of 19 cleavage-site-mimicking octapeptides, VTTXQTVP (-4 to +4), by the mature subtilisin YaB and YaB-G124A mutants...
  37. ncbi Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi
    Diogo V Lovato
    Departamento de Bioquimica, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil
    Exp Parasitol 129:362-7. 2011
    ..For the first time, a serine protease inhibitor, derived from T. infestans, was shown to impair cell invasion by T. cruzi, representing possible new target in parasite cell invasion...
  38. ncbi Kinetic analysis of salting activation of a subtilisin-like halophilic protease
    Debora N Okamoto
    Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de São Paulo Rua Três de Maio 100, 04044 20 Sao Paulo, Brazil
    Biochim Biophys Acta 1794:367-73. 2009
    ....
  39. pmc Leishmania subtilisin is a maturase for the trypanothione reductase system and contributes to disease pathology
    Ryan K Swenerton
    Department of Pathology, Sandler Center for Drug Discovery, University of California, San Francisco, California 94158, USA
    J Biol Chem 285:31120-9. 2010
    ..Furthermore, proteases are druggable targets for the development of new anti-parasitic therapy. The subtilisin protease (SUB; Clan SB, family S8) of Leishmania donovani was cloned and found to possess a unique catalytic ..
  40. pmc S1 pocket of a bacterially derived subtilisin-like protease underpins effective tissue destruction
    Wilson Wong
    Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Clayton, Victoria 3800, Australia
    J Biol Chem 286:42180-7. 2011
    The ovine footrot pathogen, Dichelobacter nodosus, secretes three subtilisin-like proteases that play an important role in the pathogenesis of footrot through their ability to mediate tissue destruction. Virulent and benign strains of D...
  41. pmc Subtilisin-like autotransporter serves as maturation protease in a bacterial secretion pathway
    L Coutte
    INSERM U447, IBL, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    EMBO J 20:5040-8. 2001
    ..SphB1 is a large exported protein with a subtilisin-like domain and a C-terminal domain typical of bacterial autotransporters...
  42. ncbi Dynamic properties of extremophilic subtilisin-like serine-proteases
    Matteo Tiberti
    Department of Biotechnology and Biosciences, University of Milano Bicocca, 20126 Milan, Italy
    J Struct Biol 174:69-83. 2011
    ..In this context, we present a comparative study of psychrophilic, mesophilic and thermophilic subtilisin-like serine proteases by all-atom molecular dynamics (MD) simulations in explicit solvent using a multiple-..
  43. ncbi Subtilisin protein inhibitor from potato tubers
    T A Revina
    Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr 33, Moscow 119071, Russia
    Biochemistry (Mosc) 69:1092-8. 2004
    ..The protein effectively inhibits the activity of subtilisin Carlsberg (Ki = 1.67 +/- 0.2 nM) by stoichiometric complexing with the enzyme at the molar ratio of 1 : 1...
  44. ncbi Requirement of a unique Ca(2+)-binding loop for folding of Tk-subtilisin from a hyperthermophilic archaeon
    Yuki Takeuchi
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochemistry 48:10637-43. 2009
    Tk-subtilisin from the hyperthermophiolic archaeon Thermococcus kodakaraensis matures from Pro-Tk-subtilisin upon autoprocessing and degradation of Tk-propeptide [Tanaka, S., Saito, K., Chon, H., Matsumura, H., Koga, Y., Takano, K...
  45. ncbi Improved growth and viability of lactobacilli in the presence of Bacillus subtilis (natto), catalase, or subtilisin
    T Hosoi
    Tokyo Metropolitan Food Technology Research Center, Japan
    Can J Microbiol 46:892-7. 2000
    ..subtilis (natto). In addition, a serine protease from B. licheniformis, subtilisin, improved the growth and viability of L. reuteri JCM 1112 and L...
  46. ncbi Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin
    Jeong Sun Kim
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Mol Biol 335:787-97. 2004
    ..A structural alignment shows a distant relationship between the PD-CD substructure of fervidolysin and pro-subtilisin E...
  47. ncbi Crystal structure of an intracellular subtilisin reveals novel structural features unique to this subtilisin family
    Jitka Vévodová
    Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5YW, UK
    Structure 18:744-55. 2010
    The intracellular subtilisin proteases (ISPs) are the only known members of the important and ubiquitous subtilisin family that function exclusively within the cell, constituting a major component of the degradome in many Gram-positive ..
  48. ncbi Cloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4
    Nack Shick Choi
    Primate Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon 305 333, Republic of Korea
    FEMS Microbiol Lett 236:325-31. 2004
    Previously, we purified a strong fibrinolytic enzyme (subtilisin DJ-4) from Bacillus sp. DJ-4 and characterized its enzymatic activity...
  49. ncbi Nucleotide and deduced amino acid sequences of a high-molecular-mass subtilisin from an alkaliphilic Bacillus isolate
    Akinori Ogawa
    Tochigi Research Laboratories of Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321 3497, Japan
    Biochim Biophys Acta 1624:109-14. 2003
    A high-molecular-mass subtilisin was found in culture broth of the alkaliphilic Bacillus sp. strain KSM-KP43...
  50. ncbi Nonaqueous biocatalytic synthesis of new cytotoxic doxorubicin derivatives: exploiting unexpected differences in the regioselectivity of salt-activated and solubilized subtilisin
    David H Altreuter
    Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 124:1871-6. 2002
    Two enzymes, Mucor javanicus lipase and subtilisin Carlsberg (SC), catalyzed the nonaqueous acylation of doxorubicin (DOX)...
  51. ncbi Nucleotide and deduced amino acid sequences of a new subtilisin from an alkaliphilic Bacillus isolate
    Katsuhisa Saeki
    Tochigi Research Laboratories of Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi, 321 3497, Japan
    Curr Microbiol 47:337-40. 2003
    The gene for a new subtilisin from the alkaliphilic Bacillus sp. KSM-LD1 was cloned and sequenced...
  52. ncbi Purification of HIV-1 virions by subtilisin digestion or CD45 immunoaffinity depletion for biochemical studies
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    Methods Mol Biol 485:15-25. 2009
    ..One approach, the subtilisin digestion procedure, enzymatically removes the proteins outside the virions...
  53. ncbi BmSI-7, a novel subtilisin inhibitor from Boophilus microplus, with activity toward Pr1 proteases from the fungus Metarhizium anisopliae
    Sergio D Sasaki
    Departamento de Bioquimica, UNIFESP EPM, Rua 3 de Maio, 100, 04044 020 Sao Paulo, SP, Brazil
    Exp Parasitol 118:214-20. 2008
    BmSI-7 and BmSI-6, two Boophilus microplus subtilisin inhibitors (BmSI) were purified and characterized from eggs...
  54. ncbi Domain inhibitory and bacteriostatic activities of the five-domain Kazal-type serine proteinase inhibitor from black tiger shrimp Penaeus monodon
    Suchao Donpudsa
    Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
    Dev Comp Immunol 33:481-8. 2009
    ..domain P1 residues T, A, E, K and E was isolated from the hemocyte cDNA libraries and found to strongly inhibit subtilisin and elastase, and weakly inhibit trypsin...
  55. ncbi Characterization of an extracellular subtilisin protease of Rhizopus microsporus and evidence for its expression during invasive rhinoorbital mycosis
    Annette Spreer
    Department of Medical Microbiology and National Reference Center for Systemic Mycoses, University Hospital of Gottingen, Gottingen, Germany
    Med Mycol 44:723-31. 2006
    ..native enzyme was obtained for the first 20 amino acids and revealed high homology to serine proteases of the subtilisin subfamily...
  56. ncbi Engineering substrate preference in subtilisin: structural and kinetic analysis of a specificity mutant
    Biao Ruan
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 47:6628-36. 2008
    Bacillus subtilisin has been a popular model protein for engineering altered substrate specificity...
  57. ncbi Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes
    A Wlodawer
    Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute at Frederick, Maryland 21702, USA
    Nat Struct Biol 8:442-6. 2001
    ..The fold of PSCP is a superset of the subtilisin fold, and the covalently bound inhibitor is linked to the enzyme through a serine residue...
  58. ncbi [Growth conditions and production of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain]
    Iu M Kirillova
    Mikrobiologiia 75:172-8. 2006
    The effect of the components of the nutrient medium on growth and production of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant strain Bacillus subtilis AJ73(pCS9) was studied...
  59. ncbi The controlled introduction of multiple negative charge at single amino acid sites in subtilisin Bacillus lentus
    B G Davis
    Department of Chemistry, University of Toronto, ON, Canada
    Bioorg Med Chem 7:2293-301. 1999
    ..the controlled incorporation of multiple negative charges at single sites in the representative serine protease, subtilisin Bacillus lentus (SBL)...
  60. ncbi Purification and molecular characterization of subtilisin-like alkaline protease BPP-A from Bacillus pumilus strain MS-1
    T Miyaji
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Tokyo, Japan
    Lett Appl Microbiol 42:242-7. 2006
    ..The present study was conducted by screening zein-degrading bacteria in an attempt to obtain zein-degrading protease...
  61. pmc The mechanical properties of chick (Gallus domesticus) sensory hair bundles: relative contributions of structures sensitive to calcium chelation and subtilisin treatment
    Mikhail E Bashtanov
    School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
    J Physiol 559:287-99. 2004
    ..and investigate changes caused by removing different links with the calcium chelator BAPTA or the protease subtilisin. Immunofluorescence with an antibody to the hair-cell antigen (HCA) and electron microscopy were used to verify ..
  62. ncbi Secreted subtilisin gene family in Trichophyton rubrum
    Olivier Jousson
    Dermatology Service DHURDV, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Gene 339:79-88. 2004
    ..A total of seven genes encoding putative serine proteases of the subtilisin family (SUB) were isolated in Trichophyton rubrum...
  63. ncbi Biochemical properties of Bacillus intermedius subtilisin-like proteinase secreted by a Bacillus subtilis recombinant strain in its stationary phase of growth
    E O Mikhailova
    Kazan State University, 420008 Kazan, Russia
    Biochemistry (Mosc) 74:308-15. 2009
    Biochemical properties of Bacillus intermedius subtilisin-like proteinase (AprBi) secreted by a B. subtilis recombinant strain in the early and late stationary phases of growth have been determined...
  64. ncbi Directed coevolution of stability and catalytic activity in calcium-free subtilisin
    Susan L Strausberg
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 44:3272-9. 2005
    We have coevolved high activity and hyperstability in subtilisin by sequentially randomizing 12 amino acid positions in calcium-free subtilisin...
  65. ncbi Identification of a cell wall-associated subtilisin-like serine protease involved in the pathogenesis of Streptococcus suis serotype 2
    Qiaoyun Hu
    College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
    Microb Pathog 48:103-9. 2010
    ..A surface-associated subtilisin-like serine protease (SspA) of S...
  66. ncbi Stabilizing mutations and calcium-dependent stability of subtilisin
    P A Alexander
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 40:10640-4. 2001
    Stability is a property of subtilisin which has proven particularly amenable to enhancement via random mutagenesis and screening, yet the effects of most stabilizing mutations are not understood in structural and energetic detail...
  67. ncbi A subtilisin-like serine protease essential for mucilage release from Arabidopsis seed coats
    Carsten Rautengarten
    Institut für Biochemie und Biologie, Genetik, Universitat Potsdam, 14476 Golm, Germany
    Plant J 54:466-80. 2008
    ..Mutant seeds carrying loss-of-function alleles of AtSBT1.7 that encodes one of 56 Arabidopsis thaliana subtilisin-like serine proteases (subtilases) do not release mucilage upon hydration...
  68. ncbi SEP-1 - a subtilisin-like serine endopeptidase from germinated seeds of Hordeum vulgare L. cv. Morex
    Debora Fontanini
    University of Wisconsin Madison, Department of Agronomy, 501 Walnut Street, Madison, WI 53726 2334, USA
    Planta 215:885-93. 2002
    ..Its amino acid sequence was similar to those of other plant subtilisin-like serine peptidases (EC 3.4.21), especially to the cucumisin-like group...
  69. pmc Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophages
    Joel A Dave
    Department of Medical Biochemistry, University of Cape Town Medical School, Observatory 7925, Cape Town, South Africa
    BMC Microbiol 2:30. 2002
    ..genes (mycP1-5) present within the genome of Mycobacterium tuberculosis H37Rv that encode a family of secreted, subtilisin-like serine proteases (the mycosins)...
  70. ncbi Subtilisin-like proteases in nematodes
    Catherine B Poole
    New England Biolabs, 240 County Road, Ipswich, MA 01938, USA
    Mol Biochem Parasitol 155:1-8. 2007
    Cleavage by subtilisin-like proteases (subtilases) is an essential step in post-translational processing of proteins found in organisms ranging from yeast to mammals...
  71. ncbi Structural insights into cold inactivation of tryptophanase and cold adaptation of subtilisin S41
    Orna Almog
    Department of Clinical Biochemistry, Ben Gurion University of the Negev, POB 653, Beer Sheva 84105, Israel
    Biopolymers 89:354-9. 2008
    ..adaptation, we have looked into the amino acid sequences and the crystal structures of two families of enzymes, subtilisin and tryptophanase...
  72. ncbi SufA--a novel subtilisin-like serine proteinase of Finegoldia magna
    Christofer Karlsson
    Division of Infection Medicine, Department of Clinical Sciences, Lund University, BMC, B14, 221 84 Lund, Sweden
    Microbiology 153:4208-18. 2007
    ..This study describes a novel subtilisin-like extracellular serine proteinase of F...
  73. pmc Control of the Streptomyces Subtilisin inhibitor gene by AdpA in the A-factor regulatory cascade in Streptomyces griseus
    Setsu Hirano
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    J Bacteriol 188:6207-16. 2006
    ..The Streptomyces subtilisin inhibitor (SSI) gene, sgiA, in S...
  74. ncbi Surfactant protein of the Streptomyces subtilisin inhibitor family inhibits transglutaminase activation in Streptomyces hygroscopicus
    Dongxu Zhang
    State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
    J Agric Food Chem 56:3403-8. 2008
    ..acid sequencing and a homology search of the purified TAPI revealed that it is a member of the Streptomyces subtilisin inhibitor (SSI) family. Furthermore, it was found that TAPI (0...
  75. ncbi Mechanism of the kinetically-controlled folding reaction of subtilisin
    Kathryn E Fisher
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 46:640-51. 2007
    Like many secreted proteases, subtilisin is kinetically stable in the mature form but unable to fold without assistance from its prodomain...
  76. ncbi Structural and mutational analyses of the interaction between the barley alpha-amylase/subtilisin inhibitor and the subtilisin savinase reveal a novel mode of inhibition
    Pernille Ollendorff Micheelsen
    Research and Development, Novozymes A S, Krogshøjvej 36, 2880 Bagsvaerd, Denmark
    J Mol Biol 380:681-90. 2008
    ..All interact with subtilisin via an exposed loop covering six interacting residues...
  77. ncbi Interaction of GroEL and GroEL/GroES complexes with a nonnative subtilisin variant: a small-angle neutron scattering study
    Susan Krueger
    NIST Center for Neutron Research, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8562, Bldg 235 Room E151, Gaithersburg, MD 20899 8562, USA
    J Struct Biol 141:240-58. 2003
    ..structure of GroEL and GroEL/GroES chaperonins complexed with a nonnative variant of the polypeptide substrate, subtilisin (PJ9)...
  78. ncbi New subtilisin-like collagenase from leaves of common plantain
    A M Bogacheva
    Moscow State University, Chemistry Department, Vorobevy Gory, B 234, 119899, Moscow, Russia
    Biochimie 83:481-6. 2001
    A new subtilisin-like proteinase hydrolyzing chromogenic peptide substrate Glp-Ala-Ala-Leu-p-nitroanilide optimally at pH 8.1 was found in common plantain leaves...
  79. ncbi Primary structure and reactive site of a novel wheat proteinase inhibitor of subtilisin and chymotrypsin
    Elia Poerio
    Dipartimento di Agrobiologia e Agrochimica, Universita della Tuscia, I 01100 Viterbo, Italy
    Biol Chem 384:295-304. 2003
    The proteinase inhibitor WSCI, active in inhibiting bacterial subtilisin and a number of animal chymotrypsins, was purified from endosperm of exaploid wheat (Triticum aestivum, c.v...
  80. ncbi Dyeing of wool fibres with natural dyes: effect of proteolytic enzymes
    Mehmet Dogru
    Department of Chemistry, Faculty of Science Art, Dicle University, Diyarbakir, Turkey
    Prep Biochem Biotechnol 36:215-21. 2006
    ..and economically obtainable and also environmentally safer for the frequently used proteolytic enzymes, such as subtilisin carlsberg, trypsin, chymotrypsin, and protease type XVI and, if possible, to prepare the specific substrate at ..
  81. ncbi A novel slow-tight binding serine protease inhibitor from eastern oyster (Crassostrea virginica) plasma inhibits perkinsin, the major extracellular protease of the oyster protozoan parasite Perkinsus marinus
    Qing Gang Xue
    Cooperative Aquatic Animal Health Research Program, Department of Veterinary Science, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
    Comp Biochem Physiol B Biochem Mol Biol 145:16-26. 2006
    ..The protein inhibited the serine proteases subtilisin A, trypsin and perkinsin, the major extracellular protease of the oyster protozoan parasite, Perkinsus marinus, ..
  82. ncbi Primary contact sites in intrinsically unstructured proteins: the case of calpastatin and microtubule-associated protein 2
    Veronika Csizmok
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Post Office Box 7, H 1518 Budapest, Hungary
    Biochemistry 44:3955-64. 2005
    ..Proteases of either narrow (trypsin, chymotrypsin, and plasmin) or broad (subtilisin and proteinase K) substrate specificity, applied at very low concentrations, preferentially cleaved both ..
  83. ncbi Purification and characterization of native and recombinant SaPIN2a, a plant sieve element-localized proteinase inhibitor
    Zhen Yu Wang
    State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat Sen University, Guangzhou 510275, China
    Plant Physiol Biochem 45:757-66. 2007
    ..Native SaPIN2a significantly inhibited serine proteinases such as trypsin, chymotrypsin, and subtilisin, with the most potent inhibitory activity on subtilisin...
  84. ncbi Nucleotide and deduced amino acid sequences of a subtilisin-like serine protease from a deep-sea bacterium, Alkalimonas collagenimarina AC40(T)
    Atsushi Kurata
    Extremobiosphere Research Center of Japan Agency for Marine Earth Science and Technology, 2 15 Natsushima, Yokosuka, 237 0061, Japan
    Appl Microbiol Biotechnol 77:311-9. 2007
    ..Based on analysis of the deduced amino acid sequence, AcpI is a subtilisin-like serine protease belonging to subtilase family A...
  85. ncbi Recombinant expression and characterization of five-domain Kazal-type serine proteinase inhibitor of black tiger shrimp (Penaeus monodon)
    Boonyarin Jarasrassamee
    Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
    Mar Biotechnol (NY) 7:46-52. 2005
    ..A 32-kDa recombinant protein (rSPIPm2) showed inhibitory activity against trypsin, chymotrypsin, and subtilisin, but not elastase...
  86. ncbi The expression of the serine proteinase gene of Bacillus intermedius in Bacillus subtilis
    Margarita Sharipova
    Department of Microbiology, Kazan State University, Kazan, Russia
    Microbiol Res 163:39-50. 2008
    ..intermedius serine proteinase expression, including the action of spo0, degU, catabolite repression and demonstrate changes in control of enzyme biosynthesis at different stages of growth...
  87. ncbi [Membrane-bound forms of serine proteases of Bacillus intermedius]
    M R Sharipova
    Kazan State University, Kazan, 420008 Tatarstan, Russia
    Mikrobiologiia 72:639-44. 2003
    ..Proteinase enzymograms revealed four molecular forms of subtilisin and four molecular forms of glutamyl endopeptidase...
  88. ncbi Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I
    Daichika Hayata
    Saito Laboratory, Research and Development, Kringle Pharma, Inc, 7 7 15 Saitoasagi, Ibaraki, Osaka 567 0085, Japan
    J Biotechnol 133:478-85. 2008
    ....
  89. ncbi A Kunitz trypsin inhibitor from chickpea (Cicer arietinum L.) that exerts anti-metabolic effect on podborer (Helicoverpa armigera) larvae
    Ajay Srinivasan
    Plant Molecular Biology Group, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India
    Plant Mol Biol 57:359-74. 2005
    ..The recombinant protein did not inhibit chymotrypsin or subtilisin activity but did exhibit stoichiometric inhibition of trypsin, comparable to soybean Kunitz trypsin inhibitor...
  90. ncbi cDNA cloning and heterologous expression of a wheat proteinase inhibitor of subtilisin and chymotrypsin (WSCI) that interferes with digestive enzymes of insect pests
    Simone Di Gennaro
    Department of Agrobiologia e Agrochimica, Universita della Tuscia, Italy
    Biol Chem 386:383-9. 2005
    A cDNA encoding the proteinase inhibitor WSCI (wheat subtilisin/chymotrypsin inhibitor) was isolated by RT-PCR...
  91. ncbi Hydrolysis of polyesters by serine proteases
    Hyun A Lim
    Institute of Agricultural Science and Technology, Chonbuk National University, Jeonju, 561 756, Korea
    Biotechnol Lett 27:459-64. 2005
    The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB)..
  92. pmc Cloning and expression of islandisin, a new thermostable subtilisin from Fervidobacterium islandicum, in Escherichia coli
    Carolin Gödde
    Institute of Technical Microbiology, Technical University Hamburg Harburg, Kasernenstr 12, D 21073 Hamburg, Germany
    Appl Environ Microbiol 71:3951-8. 2005
    A gene encoding a subtilisin-like protease, designated islandisin, from the extremely thermophilic bacterium Fervidobacterium islandicum (DSMZ 5733) was cloned and actively expressed in Escherichia coli...
  93. ncbi Conformational changes associated with receptor-stimulated guanine nucleotide exchange in a heterotrimeric G-protein alpha-subunit: NMR analysis of GTPgammaS-bound states
    Kevin D Ridge
    Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Health Science Center, Houston, TX 77030, USA
    J Biol Chem 281:7635-48. 2006
    ..Overall, these results demonstrated that R*-induced changes in G(alpha) can be followed by NMR and that guanine nucleotide exchange can be uncoupled from heterotrimer dissociation...
  94. ncbi [Biosynthesis of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain]
    Iu M Kirillova
    Mikrobiologiia 75:179-85. 2006
    The effect of certain nutrients on the growth and production of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant strain Bacillus subtilis AJ73(pCS9) was studied...
  95. ncbi Isolation and characterization of a subtilisin-like proteinase of Bacillus intermedius secreted by the Bacillus subtilis recombinant strain AJ73 at different growth stages
    E O Mikhailova
    Kazan State University, Kazan, Russia
    Biochemistry (Mosc) 72:192-8. 2007
    Two subtilisin-like serine proteinases of Bacillus intermedius secreted by the Bacillus subtilis recombinant strain AJ73 (pCS9) on the 28th and 48th h of culture growth (early and late proteinase, respectively) have been isolated by ion-..
  96. ncbi 13C- and 1H-NMR studies of oxyanion and tetrahedral intermediate stabilization by the serine proteinases: optimizing inhibitor warhead specificity and potency by studying the inhibition of the serine proteinases by peptide-derived chloromethane and glyoxa
    J P G Malthouse
    UCD School of Biomolecular and Biomedical Science, Centre for Synthesis and Chemical Biology, UCD Conway Institute, University College Dublin, Dublin 4, Ireland
    Biochem Soc Trans 35:566-70. 2007
    ..There have been extensive (13)C-NMR studies of oxyanion and tetrahedral intermediate stabilization in trypsin, subtilisin and chymotrypsin using substrate-derived chloromethane inhibitors...
  97. ncbi Construction of a 3D model of nattokinase, a novel fibrinolytic enzyme from Bacillus natto. A novel nucleophilic catalytic mechanism for nattokinase
    Zhong Liang Zheng
    Key Laboratory of Virology Wuhan University, Ministry of Education, Life Science s College of Wuhan University, Wuhan 430072, China
    J Mol Graph Model 23:373-80. 2005
    ..High-resolution X-ray structures of Subtilisin BPN' (SB), Subtilisin Carlsberg (SC), Subtilisin E (SE) and Subtilisin Savinase (SS), four proteins with ..
  98. ncbi The production of recombinant APRP, an alkaline protease derived from Bacillus pumilus TYO-67, by in vitro refolding of pro-enzyme fixed on a solid surface
    Masakazu Takahashi
    Department of Bioscience, Fukui Prefectural University, 4 1 1 Kenjojima, Fukui 910 1195
    J Biochem 136:549-56. 2004
    ..SMCE is an alkaline serine protease belonging to the subtilisin family; its candidate gene, aprP, which encodes a prepro-enzyme, was isolated in a previous study...
  99. ncbi Sol-gel immobilization of serine proteases for application in organic solvents
    D J van Unen
    Laboratory of Supramolecular Chemistry and Technology, MESA Research Institute, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
    Biotechnol Bioeng 75:154-8. 2001
    The serine proteases alpha-chymotrypsin, trypsin, and subtilisin Carlsberg were immobilized in a sol-gel matrix and the effects on the enzyme activity in organic media are evaluated...
  100. ncbi Purification and characterization of proteases from Bacillus amyloliquefaciens isolated from traditional soybean fermentation starter
    Seong Jun Cho
    Graduate School of Biotechnology, Korea University, Seoul 136 701, Korea
    J Agric Food Chem 51:7664-70. 2003
    ..These results indicate that the proteases are natural variants of subtilisin and bacillolysin.
  101. ncbi The 0.93A crystal structure of sphericase: a calcium-loaded serine protease from Bacillus sphaericus
    Orna Almog
    Department of Clinical Biochemistry, Faculty of Health Sciences, Ben Gurion University, Beer Sheva, 84105, Israel
    J Mol Biol 332:1071-82. 2003
    ..Although Sph is a mesophilic subtilisin, its amino acid sequence is similar to that of the psychrophilic subtilisins, which suggests that the crystal ..

Research Grants62

  1. Cathepsin L in the Biosynthesis of Enkephalins and beta-Endorphin Opioid Peptides
    Steven J Bark; Fiscal Year: 2012
    ..These new findings indicate a distinct cysteine protease pathway, in addition to the well known subtilisin-like Prohormone Convertases 1 and 2 for production of neuropeptides...
  2. Targeting PCSK9 Transcription to Combat Hypercholesterolemia by Berberine
    Jingwen Liu; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): PCSK9 (proprotein convertase subtilisin/kexin type 9) plays an important role in the control of circulating LDL- cholesterol (LDL-C) levels via modulation of rates of degradation of hepatic LDL ..
  3. Molecular Basis of Autosomal Dominant Hypercholesterolemia in a Multiethnic Cohor
    Zahid Ahmad; Fiscal Year: 2013
    ..in at least three genes including LDL receptor (LDLR), apolipoprotein B-100 (APOB), and proprotein convertase subtilisin-like kexin type 9 (PCSK9)...
  4. An immunoadhesin for treatment of hypercholesterolemia
    KEITH WYCOFF; Fiscal Year: 2012
    ..Proprotein convertase subtilisin/kexin type 9 (PCSK9), a protein secreted in the liver, plays a key role in maintaining cholesterol homeostasis ..
  5. Non-CD4 Host defense against P. carinii Pneumonia
    Jay K Kolls; Fiscal Year: 2013
    ..this protection Moreover this serum reacts strongly with the immunodominant antigen Kex1, as surface expressed subtilisin protease...
  6. Identification of serine hydrolases involved in Plasmodium host cell invasion
    Brooke Anderson-White; Fiscal Year: 2013
    ..In P. falciparum two essential serine hydrolases have already been identified, subtilisin-like serine protease 1 (PfSUB1) and 2 (PfSUB2)...
  7. Spatial Regulation of BMP Signaling in Dorsoventral Patterning
    David A Weisblat; Fiscal Year: 2013
    ..differences in the processing of BMP pro-peptides by Furin or other members of the pro- peptide convertase subtilisin/kexin (PCSK) family of proteases, which normally process BMP pro-peptides to their mature forms...
  8. PCSK9, Lipoprotein receptors, and Atherosclerosis
    Sergio Fazio; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Proprotein convertase subtilisin kexin type 9 (PCSK9) regulates plasma cholesterol levels by enhancing the intracellular degradation of the LDL receptor (LDLR), the primary driver of LDL clearance ..
  9. Active Immunity Targeted at PCSK9 for the Treatment of Hypercholesterolemia
    Ba Bie Teng; Fiscal Year: 2012
    ..PCSK9 (Proprotein convertase subtilisin/kexin type 9) is a newly discovered gene that has a profound effect on LDL cholesterol levels by binding to LDL ..
  10. Enzymes involved in neuropeptide biosynthesis and regulation
    Lakshmi A Devi; Fiscal Year: 2013
    ..occurs by proteolysis of the precursors at 'classical'cleavage sites involving endopeptidases of the subtilisin family...
  11. PCSK9-LDLR inhibitors from fragment-based design
    JOHN LAURENCE KULP; Fiscal Year: 2013
    DESCRIPTION: Recent phase II clinical studies demonstrate that an antibody targeting proportion convertase subtilisin/kexin type 9 (PCSK9)-a target for the treatment of hypercholesterolemia and coronary heart disease-successfully and ..
  12. Role of Proteases in Cryptosporidium-Host Cell Interactions
    Honorine Ward; Fiscal Year: 2009
    ..The overall goal of this project is to investigate the role of subtilisin-like serine proteases (subtilases) of C. parvum in host-parasite interactions...
  13. Targeting the serine protease PCSK9 via covalent complementarity
    John Chorba; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a serine protease which regulates LDL cholesterol levels and, by extension, the development of atherosclerosis...
  14. PTHRP 1 to 173 IN CHONDROCYTES
    Robert Terkeltaub; Fiscal Year: 2005
    ..In doing so, we will test the hypothesis that furin-like subtilisin family proprotein convertases (PCs) are at least partially responsible...
  15. Protease chain reactions for molecular analysis of cancer markers
    Biao Ruan; Fiscal Year: 2012
    ..Accordingly, the three experimental Aims are: 1) Engineering the subtilisin prodomain to maximize inhibition;2) Design and construct prodomain inhibitors with cleavable loops;3) Construct ..
  16. Novel Modulators of LDL Metabolism
    Nabil Elshourbagy; Fiscal Year: 2009
    ..Our therapeutic target is the interface between the protease proprotein convertase subtilisin-like kexin type 9 (PCSK9) and the low density lipoprotein receptor (LDLR), where we propose to identify and ..
  17. Towards the ab Initio Prediction of Catalytic Proficiencies of Enzymes
    Steven E Wheeler; Fiscal Year: 2010
    ..with an extensive set of experimentally determined kinetic data for wild type and mutated chorismate mutase and subtilisin proteins...
  18. RESPIRATORY ANAPHYLAXIS TO INDUSTRIAL CHEMICALS
    MERYL KAROL; Fiscal Year: 1991
    ..both immediate and late-onset pulmonary responses have been obtained to both an industrial protein allergen (subtilisin) and to a reactive industrial chemical, diphenylmethane diisocyanate (MDI)...
  19. ENZYME EFFECTOR DESIGN
    Richard Schowen; Fiscal Year: 1993
    ..The temperature dependence of the solvent isotope effect for chymotrypsin, trypsin, elastase and subtilisin will be measured to deduce the degree to which "physical" steps and "chemical" steps determine the rate, and to ..
  20. STRUCTURAL DETERMINANTS IN REGULATION OF POMC PROCESSING
    Theodore Friedman; Fiscal Year: 1999
    ..Recently PC1 and PC2, members of a new family of subtilisin-like serine proteases, the prohormone convertases, have been cloned and shown to cleave POMC in co-transfection ..
  21. Specificity and Structure: The Kex 2 and Furin Proteases
    Robert Fuller; Fiscal Year: 2005
    ..The yeast Kex2 protease is the founding member and prototype of the family of subtilisin-related proprotein processing enzymes--conserved in eukaryotes from fungi to vertebrates - that is responsible ..
  22. ACQUISITION OF NMR SPECTROMETER
    William Bachovchin; Fiscal Year: 1991
    ..The NMR studies currently focus on a-lytic protease, subtilisin, and the Polovirus 3C protease, but this laboratory is also actively working on IgA proteases and dipeptidyl ..
  23. MOLECULAR STUDIES OF HUMAN CYTOMEGALOVIRUS PROTEASE
    Liang Tong; Fiscal Year: 2003
    ..belongs to a new class of serine protease, distinct from the classical serine proteases such as chymotrypsin and subtilisin. Therefore, a new body of knowledge is needed on this new class of enzymes...
  24. PRIMARY STRUCTURE OF THE CYCLIC AMP RECEPTOR PROTEIN
    David Schlesinger; Fiscal Year: 1980
    ..In addition, core fragments of CRP produced by digestion of CRP and cAMP with subtilisin, trypsin, chymotrypsin and S...
  25. NOVEL VIRULENCE GENES OF YERSINIA
    VIRGINIA MILLER; Fiscal Year: 2003
    ..The hre-22 locus has a gene, hreP, that encodes a homologue of the subtilisin family of serine proteases. A mutation in hreP causes a 50-fold increase in the LD50...
  26. CLONING OF PNEUMOCYSTIS CARINII CHROMOSOME ENDS
    Melanie Cushion; Fiscal Year: 2002
    ..These protease genes are present in multiple copies and encode subtilisin-like serine proteases. The protease proteins are also expressed on the surface of the P. carinii organisms...
  27. Specificity of Subtilisin-like Proprotein Convertases
    JOSEPH SUCIC; Fiscal Year: 2003
    b>Subtilisin-like proprotein convertases (SPCs) are endoproteases that function within the secretory pathway of eucaryotic cells...
  28. NEUTRON STUDIES OF PROTEIN DYNAMICS AND SOLVATION
    Anthony Kossiakoff; Fiscal Year: 1991
    ..The protein, subtilisin, has been genetically engineered to alter, in a controlled way, several physicochemical properties related to ..
  29. Acquisition of a 600 MHz NMR Cryoprobe
    JOHN MARINO; Fiscal Year: 2002
    ..system include: 1) Membrane-targeting Calcium Sensors in Vision (Ames); 2) Protein Folding and Stability of Subtilisin (Bryan); 3) Structural Basis of RNA Stem-Loop Kissing Complexes (Marino); 4) Frm Genomic Sequences in Protein ..
  30. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2006
    ..Inouye's laboratory has elucidated the role of the N-terminal 77-residue propeptide in the folding of subtilisin, an alkaline serine protease...
  31. Structural examination of serpin-protein interactions
    PETER GETTINS; Fiscal Year: 2009
    ..Specific Aim 3 will determine the conformational changes that occur in caspases, cathepsins and subtilisin-like proteinases upon formation of covalent complexes with serpins...
  32. Controlled Diversity in Bacterial Stress Response
    Adam Arkin; Fiscal Year: 2009
    ..subtilis cells committing to all possible combinations of spore formation, competence for DNA transformation, subtilisin synthesis, and motility, in response to a series of stress conditions designed to exercise each part of the ..
  33. CARDIOVASCULAR LUNG, AND BLOOD IMMUNOBIOLOGY IN HEALTH
    David Corry; Fiscal Year: 2004
    ..Efficacy of CCSP will be compared to a specific protease inhibitor, streptomyces subtilisin inhibitor (SSI)...
  34. ENZYMES IN ORGANIC SYNTHESIS
    Chi Huey Wong; Fiscal Year: 1999
    ..Use genetic engineering to improve the production and performance of synthetically useful enzymes, particularly subtilisin, glycosyltransferases, and aldolases. 4...
  35. CELL ADHESION FACTOR: BIOLOGY AND CHEMISTRY
    EDWARD PEARLSTEIN; Fiscal Year: 1980
    ..b>Subtilisin digestion of fibronectin under specific conditions yields reproducible patterns of digestion products...
  36. Proteases and tags for protein purification and analysis.
    Biao Ruan; Fiscal Year: 2006
    ..The foundation for this system is a highly engineered subtilisin which hydrolyzes substrates in response to a chemical trigger...
  37. HOST CELL PROTEASE AND HIV-GP160 IN AIDS
    Alex Franzusoff; Fiscal Year: 2000
    ..During the current award, the P.I. has provided evidence that the host cell protease belongs to the subtilisin-like SPC protease family and has recently cloned the gene encoding the hPC6 protease from human T cells...
  38. UPSTREAM ACTIVATION OF BACILLUS PROMOTERS
    JAMES HOCH; Fiscal Year: 1990
    ..The site of action of the regulatory genes will be determined for the aprE (subtilisin) promoter. Extensive deletion analyses from both directions will be used to define the target sites...
  39. Label-free electrical detection of proteins
    Raj Chakrabarti; Fiscal Year: 2005
    ..I will initially test this approach by fabricating field effect sensors for the model protein subtilisin in glycerol...
  40. Proprotein Processing, Trafficking and Secretion Gordon Conference
    Nabil Seidah; Fiscal Year: 2006
    ..The integration of protease systems that are required both for normal physiology and human disease will be relevant to our understanding of the molecular bases of diabetes, cancer, Alzheimer's and drug addiction. ..
  41. BIOSYNTHESIS OF ENKEPHALIN OPIOID PEPTIDES
    Vivian Hook; Fiscal Year: 2009
    ..Arg/Lys aminopeptidase enyzmes for PE processing complement our earlier findings showing participation of the subtilisin-like PC1 and PC2 and carboxypeptidase E/H in processing PE...
  42. POMC Processing and Beta-Endorphine-Related Opioid Peptides
    Vivian Y H Hook; Fiscal Year: 2010
    ..L and Arg/Lys aminopeptidase as a new protease pathway for prohormone processing, in addition to the well known subtilisin-like PC1 and PC2 and carboxypeptidase E/H pathway...
  43. Serpin Protease Inhibitors & Opioid Neuropeptides
    Vivian Hook; Fiscal Year: 2006
    ..Serpin regulation of opioid peptides that mediate analgesia and stress may lead to development of new therapeutic approaches in pain and neurologic disease. ..
  44. Proteomics/Genomics of Opiate Analgesia and Addiction
    Vivian Hook; Fiscal Year: 2004
    ..Elucidation of distinct pathways will be significant, since it could allow future design of opiate drug regimens that provide effective analgesia, without the tolerance and dependence of addiction. ..
  45. INTEGRATED DNA SEQUENCING SYSTEM
    Vivian Hook; Fiscal Year: 2002
    ..abstract_text> ..
  46. Characterization of Microsomal Fatty Acid Elongation
    JAY HORTON; Fiscal Year: 2007
    ..abstract_text> ..
  47. Taskforce for Obesity Research at Southwestern (RMI)
    JAY HORTON; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  48. Pathogenesis of Obesity and Metabolic Syndrome
    JAY HORTON; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  49. Creation of site-specific DNA methyltransferases
    Marc Ostermeier; Fiscal Year: 2006
    ..abstract_text> ..
  50. Structure and Function of the LDL Receptor
    STEPHEN BLACKLOW; Fiscal Year: 2006
    ..Determine how the ligand-binding modules of the LDL receptor recognize apolipoprotein E (apoE)-containing ligands. 2. Elucidate the mechanism of ligand release by the LDLR at low pH. ..
  51. THE CRES GENE IN REPRODUCTION
    Gail Cornwall; Fiscal Year: 2005
    ..abstract_text> ..
  52. SERINE PROTEINASES IN APICOMPLEXAN PARASITES
    Kami Kim; Fiscal Year: 2009
    ..We have characterized two subtilisin-like serine proteinases (subtilases) from T. gondii, TgSUB1 and TgSUB2...
  53. MOLECULAR BASIS OF ENTEROCYTE REGULATION DURING ONTOGENY
    Martin Martin; Fiscal Year: 2002
    ..Analysis of the FcRn and pIgR genes offers a unique model that may lead to a better understanding of the molecular mechanism of intestinal ontogeny, and specifically the intrinsic clock which controls its regulation. ..
  54. THE CRES GENE IN REPRODUCTION
    Gail Cornwall; Fiscal Year: 2007
    ..Also, the newly purchased mass spec will enable on site protein analysis for the studies described above. In short, the environment at TTUHSC is exceptional and will fully support my proposed studies. ..
  55. Regulation of Intestinal Nutrient Transporters
    Martin Martin; Fiscal Year: 2002
    ..The identification and further characterization of these cis elements will provide better insight into the molecular mechanism of enterocyte-specific cell fate and the various factors mediating its regulation. ..
  56. MOLECULAR COMPONENTS OF INSULIN RECEPTOR BIOGENESIS
    Joseph Bass; Fiscal Year: 2003
    ..abstract_text> ..
  57. Structural Analysis of the Rhodopsin-Transducin Complex
    KEVIN RIDGE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  58. MINERALIZATION OF PRIMARY BONE
    JEFFREY GORSKI; Fiscal Year: 2008
    ..Results of this work should better define the differences between primariy and lamellar bone, as well as aid in the diagnosis and treatment of osteoporosis and atherosclerosis. ..
  59. PROTEIN FOLDING AND STABILITY OF SUBTILISIN
    Philip Bryan; Fiscal Year: 2004
    b>Subtilisin BPN is a 275 amino acid, serine protease secreted from the soil bacterium Bacillus amyloliquefaciens...
  60. GENETIC EPIDEMIOLOGY OF CHANGE IN CVD RISK FACTORS
    DAVID MICHAEL HALLMAN; Fiscal Year: 2010
    ....