neuraminidase

Summary

Summary: An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992) EC 3.2.1.18.

Top Publications

  1. ncbi Universal primer set for the full-length amplification of all influenza A viruses
    E Hoffmann
    Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105-2794, USA
    Arch Virol 146:2275-89. 2001
  2. pmc Permissive secondary mutations enable the evolution of influenza oseltamivir resistance
    Jesse D Bloom
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 328:1272-5. 2010
  3. pmc Functional balance of the hemagglutinin and neuraminidase activities accompanies the emergence of the 2009 H1N1 influenza pandemic
    Rui Xu
    Department of Molecular Biology, Scripps Research Institute, La Jolla, California, USA
    J Virol 86:9221-32. 2012
  4. pmc Influenza hemagglutinin and neuraminidase membrane glycoproteins
    Steven J Gamblin
    Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Biol Chem 285:28403-9. 2010
  5. pmc The evolutionary genetics and emergence of avian influenza viruses in wild birds
    Vivien G Dugan
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Pathog 4:e1000076. 2008
  6. pmc Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans
    Rebecca J Garten
    WHO Collaborating Center for Influenza, Centers for Disease Control and Prevention CDC, Atlanta, GA 30333, USA
    Science 325:197-201. 2009
  7. ncbi Neuraminidase inhibitors for preventing and treating influenza in healthy adults and children
    Tom Jefferson
    The Cochrane Collaboration, Roma
    Cochrane Database Syst Rev 1:CD008965. 2012
  8. pmc The genomic and epidemiological dynamics of human influenza A virus
    Andrew Rambaut
    Institute of Evolutionary Biology, University of Edinburgh, Ashworth Laboratories, Edinburgh EH9 3JT, UK
    Nature 453:615-9. 2008
  9. ncbi Neuraminidase inhibitors for influenza
    Anne Moscona
    Department of Pediatrics, Weill Medical College of Cornell University, New York, NY 10021, USA
    N Engl J Med 353:1363-73. 2005
  10. pmc Crystal structures of two subtype N10 neuraminidase-like proteins from bat influenza A viruses reveal a diverged putative active site
    Xueyong Zhu
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 109:18903-8. 2012

Detail Information

Publications329 found, 100 shown here

  1. ncbi Universal primer set for the full-length amplification of all influenza A viruses
    E Hoffmann
    Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105-2794, USA
    Arch Virol 146:2275-89. 2001
    ..For N3, N6, N7, and N9 subtypes, the segment specific sequences of the neuraminidase genes are different...
  2. pmc Permissive secondary mutations enable the evolution of influenza oseltamivir resistance
    Jesse D Bloom
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 328:1272-5. 2010
    The His274-->Tyr274 (H274Y) mutation confers oseltamivir resistance on N1 influenza neuraminidase but had long been thought to compromise viral fitness...
  3. pmc Functional balance of the hemagglutinin and neuraminidase activities accompanies the emergence of the 2009 H1N1 influenza pandemic
    Rui Xu
    Department of Molecular Biology, Scripps Research Institute, La Jolla, California, USA
    J Virol 86:9221-32. 2012
    ..progenitors and human viruses from the 2009 pandemic and measured the activities of the hemagglutinin (HA) and neuraminidase (NA), which are the two viral surface proteins that interact with host glycan receptors...
  4. pmc Influenza hemagglutinin and neuraminidase membrane glycoproteins
    Steven J Gamblin
    Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Biol Chem 285:28403-9. 2010
    ..Anti-influenza drugs mimic the natural sialic acid substrate of the virus neuraminidase enzyme but utilize the much tighter binding of the drugs for efficacy...
  5. pmc The evolutionary genetics and emergence of avian influenza viruses in wild birds
    Vivien G Dugan
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Pathog 4:e1000076. 2008
    ..These isolates represented 29 type A influenza virus hemagglutinin (HA) and neuraminidase (NA) subtype combinations, with up to 26% of isolates showing evidence of mixed subtype infection...
  6. pmc Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans
    Rebecca J Garten
    WHO Collaborating Center for Influenza, Centers for Disease Control and Prevention CDC, Atlanta, GA 30333, USA
    Science 325:197-201. 2009
    ..Antigenically the viruses are homogeneous and similar to North American swine A(H1N1) viruses but distinct from seasonal human A(H1N1)...
  7. ncbi Neuraminidase inhibitors for preventing and treating influenza in healthy adults and children
    Tom Jefferson
    The Cochrane Collaboration, Roma
    Cochrane Database Syst Rev 1:CD008965. 2012
    Planning for outbreaks of influenza is a high priority public health issue for national governments. Neuraminidase inhibitors (NIs) are thought to help reduce the symptoms of influenza with several possible mechanisms proposed...
  8. pmc The genomic and epidemiological dynamics of human influenza A virus
    Andrew Rambaut
    Institute of Evolutionary Biology, University of Edinburgh, Ashworth Laboratories, Edinburgh EH9 3JT, UK
    Nature 453:615-9. 2008
    ..These results suggest a sink-source model of viral ecology in which new lineages are seeded from a persistent influenza reservoir, which we hypothesize to be located in the tropics, to sink populations in temperate regions...
  9. ncbi Neuraminidase inhibitors for influenza
    Anne Moscona
    Department of Pediatrics, Weill Medical College of Cornell University, New York, NY 10021, USA
    N Engl J Med 353:1363-73. 2005
  10. pmc Crystal structures of two subtype N10 neuraminidase-like proteins from bat influenza A viruses reveal a diverged putative active site
    Xueyong Zhu
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 109:18903-8. 2012
    Recently, we reported a unique influenza A virus subtype H17N10 from little yellow-shouldered bats. Its neuraminidase (NA) gene encodes a protein that appears to be highly divergent from all known influenza NAs and was assigned as a new ..
  11. pmc Oseltamivir-resistant pandemic A/H1N1 virus is as virulent as its wild-type counterpart in mice and ferrets
    Marie Eve Hamelin
    CHUQ CHUL Research Center in Infectious Diseases and Laval University, Quebec City, Quebec, Canada
    PLoS Pathog 6:e1001015. 2010
    The neuraminidase inhibitor oseltamivir is currently used for treatment of patients infected with the pandemic A/H1N1 (pH1N1) influenza virus, although drug-resistant mutants can emerge rapidly and possibly be transmitted...
  12. ncbi Influenza virus resistance to neuraminidase inhibitors
    Melanie Samson
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antiviral Res 98:174-85. 2013
    ..The purpose of this article is to review the mechanism of action, pharmacokinetics and clinical indications of neuraminidase inhibitors (NAIs) with an emphasis on the emergence of antiviral drug resistance...
  13. pmc Structural and functional characterization of neuraminidase-like molecule N10 derived from bat influenza A virus
    Qing Li
    School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
    Proc Natl Acad Sci U S A 109:18897-902. 2012
    ..It also identifies a neuraminidase (NA)-like protein, N10, that is highly divergent from the nine other well-established serotypes of influenza A ..
  14. pmc Systematic review of influenza resistance to the neuraminidase inhibitors
    Kristian Thorlund
    Department of Clinical Epidemiology and Biostatistics, Faculty of Health Sciences, McMaster University, Hamilton, L8N 3Z5 Ontario, Canada
    BMC Infect Dis 11:134. 2011
    Antivirals play a critical role in the prevention and the management of influenza. One class of antivirals, neuraminidase inhibitors (NAIs), is effective against all human influenza viruses...
  15. pmc Antibody correlates and predictors of immunity to naturally occurring influenza in humans and the importance of antibody to the neuraminidase
    Robert B Couch
    Departments of Molecular Virology and Microbiology and Medicine, Baylor College of Medicine, One Baylor Plaza, MS BCM280, Houston, TX 77030, USA
    J Infect Dis 207:974-81. 2013
    ..Serum antibody to the hemagglutinin (HA) of influenza viruses is a correlate and predictor of immunity to influenza in humans; the relative values of other correlates are uncertain...
  16. pmc A contributing role for anti-neuraminidase antibodies on immunity to pandemic H1N1 2009 influenza A virus
    Glendie Marcelin
    Department of Infectious Diseases, Division of Virology, St Jude Children s Research Hospital, Memphis, Tennessee, United States of America
    PLoS ONE 6:e26335. 2011
    ..The impact of antibodies to the neuraminidase (NA) of seasonal influenza A viruses to cross-immunity against pH1N1 infection is unknown.
  17. pmc Evidence of person-to-person transmission of oseltamivir-resistant pandemic influenza A(H1N1) 2009 virus in a hematology unit
    Catherine Moore
    Public Health Wales Microbiology, Public Health Wales NHS Trust, Cardiff, UK
    J Infect Dis 203:18-24. 2011
    ..infection were identified, of which, ten were related as shown by sequence analysis of the hemagglutinin and neuraminidase genes...
  18. ncbi Initial genetic characterization of the 1918 "Spanish" influenza virus
    J K Taubenberger
    Division of Molecular Pathology, Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington DC 20306 6000, USA
    Science 275:1793-6. 1997
    ..Nine fragments of viral RNA were sequenced from the coding regions of hemagglutinin, neuraminidase, nucleoprotein, matrix protein 1, and matrix protein 2...
  19. pmc Pre-existing immunity against swine-origin H1N1 influenza viruses in the general human population
    Jason A Greenbaum
    Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:20365-70. 2009
    ..H1N1 strains are conserved in the S-OIV, with only 17% (1/6) conserved in the hemagglutinin (HA) and neuraminidase (NA) surface proteins...
  20. pmc Role of permissive neuraminidase mutations in influenza A/Brisbane/59/2007-like (H1N1) viruses
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    PLoS Pathog 7:e1002431. 2011
    b>Neuraminidase (NA) mutations conferring resistance to NA inhibitors were believed to compromise influenza virus fitness...
  21. pmc Structural and functional analysis of laninamivir and its octanoate prodrug reveals group specific mechanisms for influenza NA inhibition
    Christopher J Vavricka
    CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    PLoS Pathog 7:e1002249. 2011
    The 2009 H1N1 influenza pandemic (pH1N1) led to record sales of neuraminidase (NA) inhibitors, which has contributed significantly to the recent increase in oseltamivir-resistant viruses...
  22. pmc Prevalence of epistasis in the evolution of influenza A surface proteins
    Sergey Kryazhimskiy
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    PLoS Genet 7:e1001301. 2011
    ..We apply this method to the surface proteins hemagglutinin and neuraminidase of influenza A virus subtypes H3N2 and H1N1...
  23. pmc Transport of influenza virus neuraminidase (NA) to host cell surface is regulated by ARHGAP21 and Cdc42 proteins
    Song Wang
    CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences CAS, Beijing 100101, China
    J Biol Chem 287:9804-16. 2012
    Influenza virus neuraminidase (NA) is transported to the virus assembly site at the plasma membrane and is a major viral envelope component that plays a critical role in the release of progeny virions and in determination of host range ..
  24. ncbi Neuraminidase inhibitor resistance in influenza viruses and laboratory testing methods
    Ha T Nguyen
    Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, USA
    Antivir Ther 17:159-73. 2012
    ..Currently, two classes of drugs, adamantanes (M2 blockers) and neuraminidase (NA) inhibitors (NAIs), are available for treatment and chemoprophylaxis of influenza infections...
  25. pmc Generation of replication-competent recombinant influenza A viruses carrying a reporter gene harbored in the neuraminidase segment
    Feng Li
    Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    J Virol 84:12075-81. 2010
    ..In this study, two recombinant influenza A viruses with a neuraminidase (NA) segment harboring enhanced green fluorescent protein (EGFP) in the background of A/PR/8/34 (PR8) were ..
  26. pmc Systematic identification of H274Y compensatory mutations in influenza A virus neuraminidase by high-throughput screening
    Nicholas C Wu
    Department of Molecular and Medical Pharmacology, University of California, Los Angeles David Geffen School of Medicine, Los Angeles, CA, USA
    J Virol 87:1193-9. 2013
    Compensatory mutations contribute to the appearance of the oseltamivir resistance substitution H274Y in the neuraminidase (NA) gene of H1N1 influenza viruses...
  27. pmc Contribution of antibody production against neuraminidase to the protection afforded by influenza vaccines
    Glendie Marcelin
    Department of Infectious Diseases, Division Virology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Rev Med Virol 22:267-79. 2012
    ..Antibodies raised against both major viral surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA), can contribute to protective immunity...
  28. pmc Generation and characterization of recombinant pandemic influenza A(H1N1) viruses resistant to neuraminidase inhibitors
    Andrés Pizzorno
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 203:25-31. 2011
    b>Neuraminidase inhibitors (NAIs) play a key role in the management of influenza epidemics and pandemics...
  29. pmc Characterization of oseltamivir-resistant 2009 H1N1 pandemic influenza A viruses
    Maki Kiso
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato ku, Tokyo, Japan
    PLoS Pathog 6:e1001079. 2010
    ..treatment in many countries of patients infected with 2009 pandemic influenza A (H1N1) viruses with the neuraminidase (NA) inhibitors oseltamivir and zanamivir has led to the emergence of pandemic strains resistant to these ..
  30. pmc Oseltamivir-resistant pandemic H1N1/2009 influenza virus possesses lower transmissibility and fitness in ferrets
    Susu Duan
    Division of Virology, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    PLoS Pathog 6:e1001022. 2010
    The neuraminidase (NA) inhibitor oseltamivir offers an important immediate option for the control of influenza, and its clinical use has increased substantially during the recent H1N1 pandemic...
  31. pmc Mutations in a conserved residue in the influenza virus neuraminidase active site decreases sensitivity to Neu5Ac2en-derived inhibitors
    J L McKimm-Breschkin
    Biomolecular Research Institute, Parkville, Australia
    J Virol 72:2456-62. 1998
    The influenza virus neuraminidase (NA)-specific inhibitor zanamivir (4-guanidino-Neu5Ac2en) is effective in humans when administered topically within the respiratory tract...
  32. ncbi Avian influenza virus exhibits rapid evolutionary dynamics
    Rubing Chen
    Center for Infectious Disease Dynamics, Department of Biology, The Pennsylvania State University, PA, USA
    Mol Biol Evol 23:2336-41. 2006
    ..Using the same approach, we also estimated that the common ancestors of the hemagglutinin and neuraminidase sequences of AIV arose within the last 3,000 years, with most intrasubtype diversity emerging within the last ..
  33. pmc Antigenic fingerprinting of H5N1 avian influenza using convalescent sera and monoclonal antibodies reveals potential vaccine and diagnostic targets
    Surender Khurana
    Division of Viral Products, Center for Biologics Evaluation and Research CBER, Food and Drug Administration, Bethesda, Maryland, USA
    PLoS Med 6:e1000049. 2009
    ..Characterizing the B cell responses in convalescent individuals could help in the design of future vaccines and therapeutics...
  34. pmc In vitro assessment of attachment pattern and replication efficiency of H5N1 influenza A viruses with altered receptor specificity
    Salin Chutinimitkul
    Department of Virology and National Influenza Center, Erasmus MC, P O Box 2040, 3000CA Rotterdam, Netherlands
    J Virol 84:6825-33. 2010
    ....
  35. ncbi The 2009 pandemic H1N1 neuraminidase N1 lacks the 150-cavity in its active site
    Qing Li
    CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Nat Struct Mol Biol 17:1266-8. 2010
    Influenza A virus neuraminidase can be classified into groups 1 and 2 on the basis of its primary structure. The main structural feature of group 1 neuraminidase is an extra cavity in the active site, the 150-cavity...
  36. pmc Ensemble-based virtual screening reveals potential novel antiviral compounds for avian influenza neuraminidase
    Lily S Cheng
    National Biomedical Computation Resource, University of California, San Diego, La Jolla, California 92093, USA
    J Med Chem 51:3878-94. 2008
    ..representative receptor ensembles extracted from explicitly solvated molecular dynamics simulations of the neuraminidase system...
  37. ncbi Tissue-specific contributions of pneumococcal virulence factors to pathogenesis
    Carlos J Orihuela
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    J Infect Dis 190:1661-9. 2004
    ..A (CbpA), pneumolysin (Pln), pyruvate oxidase (SpxB), autolysin (LytA), pneumococcal surface protein A, or neuraminidase A (NanA) to replicate in distinct anatomical sites and translocate from one site to the next...
  38. ncbi Sialic acid: a preventable signal for pneumococcal biofilm formation, colonization, and invasion of the host
    Claudia Trappetti
    Laboratorio di Microbiologia Molecolare e Biotecnologia, Dipartimento di Biologia Molecolare, Universita di Siena, Siena, Italy
    J Infect Dis 199:1497-505. 2009
    ..Competition of both sialic acid-dependent phenotypes was found to be successful when evaluated using the neuraminidase inhibitors DANA (i.e., 2,3-didehydro-2-deoxy-N-acetylneuraminic acid), zanamivir, and oseltamivir...
  39. pmc Influenza virus hemagglutinin and neuraminidase, but not the matrix protein, are required for assembly and budding of plasmid-derived virus-like particles
    Benjamin J Chen
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208 3500, USA
    J Virol 81:7111-23. 2007
    ..Additionally, hemagglutinin (HA), when treated with exogenous neuraminidase (NA) or coexpressed with viral NA, could be released from cells independently of M1...
  40. pmc Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
    Bruna C G de Alencar
    Centro Interdisciplinar de Terapia Gênica CINTERGEN, Universidade Federal de Sao Paulo Escola Paulista de Medicina, Rua Mirassol 207, Sao Paulo, SP 04044 010, Brazil
    Infect Immun 77:4383-95. 2009
    ..Our study thus demonstrated a role for perforin and IFN-gamma in a number of T-cell-mediated effector functions and in the antiparasitic immunity generated by a heterologous plasmid DNA prime-adenovirus boost vaccination strategy...
  41. ncbi Respiratory viruses predisposing to bacterial infections: role of neuraminidase
    Ville T Peltola
    Department of Infectious Diseases, St Judes Children s Research Hospital, Memphis, TN, USA
    Pediatr Infect Dis J 23:S87-97. 2004
    ..This article focuses on viral and bacterial combinations, particularly synergism between influenza and Streptococcus pneumoniae...
  42. pmc Combinatorial effect of two framework mutations (E119V and I222L) in the neuraminidase active site of H3N2 influenza virus on resistance to oseltamivir
    Mathilde Richard
    Laboratoire Virologie et Pathologie Humaine VirPath, CNRS FRE 3011 UCBL1, Universite de Lyon, Faculté de médecine RTH Laennec, 7 11 rue Guillaume Paradin, 69372 Lyon Cedex 08, France
    Antimicrob Agents Chemother 55:2942-52. 2011
    b>Neuraminidase (NA) inhibitors (NIs) are the first line of defense against influenza virus. Reverse genetics experiments allow the study of resistance mechanisms by anticipating the impacts of mutations to the virus...
  43. pmc Structural characterization of the 1918 influenza virus H1N1 neuraminidase
    Xiaojin Xu
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 82:10493-501. 2008
    Influenza virus neuraminidase (NA) plays a crucial role in facilitating the spread of newly synthesized virus in the host and is an important target for controlling disease progression...
  44. pmc A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus
    Alejandra Castillo Alvarez
    Biochip Innovations Pty Ltd, 8 Mile Plains, Queensland, Australia
    Virol J 5:77. 2008
    ..Type A virus can be classified into subtypes according to the viral envelope glycoproteins, hemagglutinin and neuraminidase. Here we review the existing specificity and amplification of published primers to subtype neuraminidase genes ..
  45. ncbi Simultaneous quantification of hemagglutinin and neuraminidase of influenza virus using isotope dilution mass spectrometry
    Tracie L Williams
    Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, United States
    Vaccine 30:2475-82. 2012
    ..There is currently no regulatory method that quantifies neuraminidase (NA), the other major membrane-bound protein thought to have protective capability...
  46. pmc Influenza B viruses with mutation in the neuraminidase active site, North Carolina, USA, 2010-11
    Katrina Sleeman
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 17:2043-6. 2011
    ..and characterize a cluster of influenza B viruses circulating in North Carolina with a mutation in the neuraminidase active site that may reduce susceptibility to oseltamivir and the investigational drug peramivir but not to ..
  47. ncbi Novel H1N2 swine influenza reassortant strain in pigs derived from the pandemic H1N1/2009 virus
    Ana Moreno
    Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia Romagna IZSLER, Via Bianchi, 9, 25124 Brescia, Italy
    Vet Microbiol 149:472-7. 2011
    ..Phylogenetic analysis showed that all of the genes of A/Sw/It/116114/2010, with the exception of neuraminidase (NA), belonged to the H1N1pdm cluster...
  48. ncbi Global assessment of resistance to neuraminidase inhibitors, 2008-2011: the Influenza Resistance Information Study (IRIS)
    Richard J Whitley
    Department of Pediatrics, Microbiology, Medicine and Neurosurgery, University of Alabama at Birmingham, 303 CHB, 1600 Seventh Ave S, Birmingham, AL 35233, USA
    Clin Infect Dis 56:1197-205. 2013
    ..influenza A(H1N1) viruses, a global observational investigation, the Influenza Resistance Information Study (IRIS; NCT00884117), was initiated in 2008 to study neuraminidase inhibitor (NAI) resistance and clinical outcome.
  49. pmc Neuraminidase inhibitors for treatment and prophylaxis of influenza in children: systematic review and meta-analysis of randomised controlled trials
    Matthew Shun-Shin
    Kadoorie Centre, John Radcliffe Hospital, Headington, Oxford OX3 9DU
    BMJ 339:b3172. 2009
    To assess the effects of the neuraminidase inhibitors oseltamivir and zanamivir in treatment of children with seasonal influenza and prevention of transmission to children in households.
  50. pmc Neuraminidase-inhibiting antibody is a correlate of cross-protection against lethal H5N1 influenza virus in ferrets immunized with seasonal influenza vaccine
    Steven Rockman
    CSL Limited, Parkville, Victoria, Australia
    J Virol 87:3053-61. 2013
    ..although the hemagglutinin contributed to protection, the dominant protective response was targeted toward the neuraminidase (NA) and correlated with sialic acid cleavage-inhibiting antibody titers...
  51. pmc Impact of neuraminidase inhibitor treatment on outcomes of public health importance during the 2009-2010 influenza A(H1N1) pandemic: a systematic review and meta-analysis in hospitalized patients
    Stella G Muthuri
    Health Protection and Influenza Research Group, Division of Epidemiology and Public Health, University of Nottingham, United Kingdom
    J Infect Dis 207:553-63. 2013
    The impact of neuraminidase inhibitor (NAI) treatment on clinical outcomes of public health importance during the 2009-2010 pandemic has not been firmly established.
  52. ncbi Evolution of highly pathogenic avian influenza H5N1 viruses in Egypt indicating progressive adaptation
    A Arafa
    National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, Giza, Egypt
    Arch Virol 157:1931-47. 2012
    ..Genetic analysis of the neuraminidase (NA) gene and the other six internal genes indicates a grouping of the Egyptian viruses similar to that ..
  53. ncbi Efficient protective immunity against Trypanosoma cruzi infection after nasal vaccination with recombinant Sendai virus vector expressing amastigote surface protein-2
    Xuefeng Duan
    Department of Parasitology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Vaccine 27:6154-9. 2009
    ..cruzi are interesting candidates for the development of a new mode of recombinant viral vaccine against Chagas' disease...
  54. pmc The value of neuraminidase inhibitors for the prevention and treatment of seasonal influenza: a systematic review of systematic reviews
    Barbara Michiels
    Department of Primary and Interdisciplinary Care Antwerp, Centre for General Practice, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium
    PLoS ONE 8:e60348. 2013
    Controversy has arisen regarding the effectiveness of neuraminidase inhibitors (NIs), especially against influenza-related complications...
  55. pmc Molecular basis for broad neuraminidase immunity: conserved epitopes in seasonal and pandemic H1N1 as well as H5N1 influenza viruses
    Hongquan Wan
    Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    J Virol 87:9290-300. 2013
    Influenza A viruses, including H1N1 and H5N1 subtypes, pose a serious threat to public health. Neuraminidase (NA)-related immunity contributes to protection against influenza virus infection...
  56. pmc Neuraminidase inhibitor sensitivity and receptor-binding specificity of Cambodian clade 1 highly pathogenic H5N1 influenza virus
    M Naughtin
    Institut Pasteur in Cambodia, Virology Unit, 5 Monivong blvd, P O Box 983, Phnom Penh, Cambodia
    Antimicrob Agents Chemother 55:2004-10. 2011
    ..We have observed naturally occurring mutations in hemagglutinin (HA) and neuraminidase (NA) of Cambodian H5N1 viruses that were predicted to alter sensitivity to neuraminidase inhibitors (NAIs) and/..
  57. pmc Genomic analyses, gene expression and antigenic profile of the trans-sialidase superfamily of Trypanosoma cruzi reveal an undetected level of complexity
    Leandro M Freitas
    Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
    PLoS ONE 6:e25914. 2011
    ..cruzi epitopes during infection. Taken together, our results contribute to a better understanding of the real complexity of the TcS family and open new avenues for investigating novel roles of this family during T. cruzi infection...
  58. pmc Emergence of H5N1 avian influenza viruses with reduced sensitivity to neuraminidase inhibitors and novel reassortants in Lao People's Democratic Republic
    David A Boltz
    Division of Virology, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    J Gen Virol 91:949-59. 2010
    ..Of greatest concern was the circulation of three isolates that showed reduced sensitivity to the neuraminidase (NA) inhibitor oseltamivir in an enzyme inhibition assay, each with different NA mutations - V116A, I222L and ..
  59. ncbi Oseltamivir resistance and the H274Y neuraminidase mutation in seasonal, pandemic and highly pathogenic influenza viruses
    Aeron C Hurt
    WHO Collaborating Centre for Reference and Research on Influenza, Melbourne, Victoria, Australia
    Drugs 69:2523-31. 2009
    ..These drugs - oseltamivir (Tamiflu) and zanamivir (Relenza) - are classified as neuraminidase inhibitors (NAIs) because they act by inhibiting one of the key surface proteins of the influenza virus, the ..
  60. pmc The surface-anchored NanA protein promotes pneumococcal brain endothelial cell invasion
    Satoshi Uchiyama
    Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA
    J Exp Med 206:1845-52. 2009
    ..All SPN strains possess a gene encoding the surface-anchored sialidase (neuraminidase) NanA, which cleaves sialic acid on host cells and proteins...
  61. ncbi Three Streptococcus pneumoniae sialidases: three different products
    Guogang Xu
    Biomedical Sciences Research Complex, University of St Andrews, KY16 9ST, UK
    J Am Chem Soc 133:1718-21. 2011
    ..We propose that the three pneumococcal sialidases share a common catalytic mechanism up to the final product formation step, and speculate on the roles of the enzymes in the lifecycle of the bacterium...
  62. ncbi Treatment of epidemic and pandemic influenza with neuraminidase and M2 proton channel inhibitors
    J S Oxford
    Academic and Retroscreen Virology, Barts and The London, Queen Mary s School of Medicine and Dentistry, London, UK
    Clin Microbiol Infect 9:1-14. 2003
    ..of anti-influenza drugs now exists, and includes the M2 blockers (amantadine and rimantadine) and the neuraminidase inhibitors (Relenza and Tamiflu)...
  63. ncbi Susceptibility of human influenza viruses from Australasia and South East Asia to the neuraminidase inhibitors zanamivir and oseltamivir
    Aeron C Hurt
    WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, VIC 3052, Australia
    Antiviral Res 62:37-45. 2004
    ..Based on median IC50 values, influenza A viruses (with neuraminidase subtypes N1 and N2) were more sensitive to both the NA inhibitors than were influenza B strains...
  64. ncbi Effect of the neuraminidase mutation H274Y conferring resistance to oseltamivir on the replicative capacity and virulence of old and recent human influenza A(H1N1) viruses
    Mariana Baz
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 201:740-5. 2010
    The viral fitness of neuraminidase inhibitor (NAI)-resistant influenza viruses is believed to be impaired...
  65. ncbi Measurement of neutralizing antibody responses against H5N1 clades in immunized mice and ferrets using pseudotypes expressing influenza hemagglutinin and neuraminidase
    Cheguo Tsai
    The Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
    Vaccine 27:6777-90. 2009
    ..Toward this goal, we generated a panel of pseudotypes expressing influenza hemagglutinin (HA) and neuraminidase (NA) and developed a pseudotype-based neutralization (PN) assay...
  66. pmc A two-year survey of the oseltamivir-resistant influenza A(H1N1) virus in Yamagata, Japan and the clinical effectiveness of oseltamivir and zanamivir
    Yoko Matsuzaki
    Course of Clinical Nursing, Yamagata University Faculty of Medicine, Yamagata 990 9585, Japan
    Virol J 7:53. 2010
    ....
  67. pmc CD8+-T-cell-dependent control of Trypanosoma cruzi infection in a highly susceptible mouse strain after immunization with recombinant proteins based on amastigote surface protein 2
    Adriano F S Araújo
    CINTERGEN, UNIFESP Escola Paulista de Medicina, Rua Mirassol, 207, São Paulo SP 04044 010, Brazil
    Infect Immun 73:6017-25. 2005
    ..cruzi infection...
  68. pmc Neuraminidase receptor binding variants of human influenza A(H3N2) viruses resulting from substitution of aspartic acid 151 in the catalytic site: a role in virus attachment?
    Yi Pu Lin
    Virology Division, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Virol 84:6769-81. 2010
    ..an antigenic change in hemagglutinin (HA) but was shown to be the result of a mutation in aspartic acid 151 of neuraminidase (NA) to glycine, asparagine, or alanine, which caused an oseltamivir-sensitive agglutination of RBCs...
  69. ncbi Long-term protective immunity induced against Trypanosoma cruzi infection after vaccination with recombinant adenoviruses encoding amastigote surface protein-2 and trans-sialidase
    Alexandre V Machado
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270 910, Brazil
    Hum Gene Ther 17:898-908. 2006
    ..Taking together, these results show that recombinant adenoviruses expressing TS and ASP-2 antigens of T. cruzi are interesting candidates for the development of a vaccine against Chagas' disease...
  70. pmc Neuraminidase stalk length and additional glycosylation of the hemagglutinin influence the virulence of influenza H5N1 viruses for mice
    Yumiko Matsuoka
    Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Virol 83:4704-8. 2009
    ..H5 and H7 viruses in poultry, the hemagglutinin (HA) can acquire additional glycosylation sites, and the neuraminidase (NA) stalk becomes shorter...
  71. pmc The NanA neuraminidase of Streptococcus pneumoniae is involved in biofilm formation
    Dane Parker
    Department of Pediatrics and Pharmacology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Infect Immun 77:3722-30. 2009
    ..The neuraminidase of S...
  72. ncbi Drug design against a shifting target: a structural basis for resistance to inhibitors in a variant of influenza virus neuraminidase
    J N Varghese
    Biomolecular Research Institute 343 Royal Parade, Parkville, 3052, Australia
    Structure 6:735-46. 1998
    Inhibitors of the influenza virus neuraminidase have been shown to be effective antiviral agents in humans...
  73. pmc Proteolytic activation of the 1918 influenza virus hemagglutinin
    Chawaree Chaipan
    Nikolaus Fiebiger Center for Molecular Medicine, University Hospital Erlangen, 91054 Erlangen, Germany
    J Virol 83:3200-11. 2009
    ..The neuraminidase (NA) protein of the 1918 influenza virus allows trypsin-independent growth in canine kidney cells (MDCK)...
  74. ncbi Role of neuraminidase in lethal synergism between influenza virus and Streptococcus pneumoniae
    Jonathan A McCullers
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    J Infect Dis 187:1000-9. 2003
    ..Using a model of viral-bacterial synergism, we assessed the role that the influenza virus neuraminidase (NA) has in priming mice for pneumococcal infection...
  75. ncbi Remarkable loop flexibility in avian influenza N1 and its implications for antiviral drug design
    Rommie E Amaro
    Department of Chemistry and Biochemistry, NSF Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California 92093 0365, USA
    J Am Chem Soc 129:7764-5. 2007
  76. pmc Genetic diversity of the 2009 pandemic influenza A(H1N1) viruses in Finland
    Niina Ikonen
    Viral Infections Unit, Department of Vaccination and Immune Protection, National Institute for Health and Welfare THL, Helsinki, Finland
    PLoS ONE 5:e13329. 2010
    ..In September 2009 the pandemic virus started to spread in the general population, leading to localized outbreaks and peak epidemic activity was reached during weeks 43-48...
  77. pmc Transforming growth factor-β: activation by neuraminidase and role in highly pathogenic H5N1 influenza pathogenesis
    Christina M Carlson
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin, United States of America
    PLoS Pathog 6:e1001136. 2010
    ..shown that TGF-β activity increases during influenza virus infection in mice and suggested that the neuraminidase (NA) protein mediates this activation...
  78. pmc Growth of Streptococcus pneumoniae on human glycoconjugates is dependent upon the sequential activity of bacterial exoglycosidases
    Amanda M Burnaugh
    Center for Microbial Pathogenesis, Research Institute at Nationwide Children s Hospital, and Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio 43205 2696, USA
    J Bacteriol 190:221-30. 2008
    ..Three exoglycosidases, a neuraminidase (NanA), beta-galactosidase (BgaA), and N-acetylglucosaminidase (StrH), have been previously demonstrated to ..
  79. ncbi The Trypanosoma cruzi proteome
    J A Atwood
    Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA
    Science 309:473-6. 2005
    ..The four parasite stages appear to use distinct energy sources, including histidine for stages present in the insect vectors and fatty acids by intracellular amastigotes...
  80. pmc Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression
    Yoichiro Kakugawa
    Division of Biochemistry, Research Institute, Department of Surgery, Miyagi Prefectural Cancer Center, Natori, Miyagi 981 1293, Japan
    Proc Natl Acad Sci U S A 99:10718-23. 2002
    ..This finding provides a possible sialidase target for diagnosis and therapy of colon cancer...
  81. pmc Prediction of biological functions on glycosylation site migrations in human influenza H1N1 viruses
    Shisheng Sun
    Laboratory of Functional Glycomics, College of Life Sciences, Northwest University, Xi an, People s Republic of China
    PLoS ONE 7:e32119. 2012
    ..to more effectively mask the antigenic sites, to more effectively protect the enzymatic cleavage sites of neuraminidase (NA), to stabilize the polymeric structures, to regulate the receptor binding and catalytic activities and to ..
  82. pmc Mechanism of 150-cavity formation in influenza neuraminidase
    Rommie E Amaro
    Department of Pharmaceutical Sciences, Computer Science and Chemistry, University of California, Irvine, California 92697, USA
    Nat Commun 2:388. 2011
    The recently discovered 150-cavity in the active site of group-1 influenza A neuraminidase (NA) proteins provides a target for rational structure-based drug development to counter the increasing frequency of antiviral resistance in ..
  83. ncbi Very low pandemic influenza A (H1N1) 2009 mortality associated with early neuraminidase inhibitor treatment in Japan: analysis of 1000 hospitalized children
    Norio Sugaya
    Department of Paediatrics, Keiyu Hospital, 3 7 3 Minatomirai, Nishi ku, Yokohama, 220 0012 Kanagawa, Japan
    J Infect 63:288-94. 2011
    ..We analyzed the clinical manifestations and treatment of children hospitalized because of H1N1/09 infection in order to clarify the association between treatment with neuraminidase inhibitors and the low mortality rate.
  84. ncbi Telomere and subtelomere of Trypanosoma cruzi chromosomes are enriched in (pseudo)genes of retrotransposon hot spot and trans-sialidase-like gene families: the origins of T. cruzi telomeres
    Dong Kim
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862, CEP 04023 062, S Paulo, Brazil
    Gene 346:153-61. 2005
    ..cruzi. Finally, a mechanism for generation of T. cruzi telomere by chromosome breakage and telomere healing is proposed...
  85. pmc Plasminogen-binding activity of neuraminidase determines the pathogenicity of influenza A virus
    H Goto
    Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
    J Virol 75:9297-301. 2001
    When expressed in vitro, the neuraminidase (NA) of A/WSN/33 (WSN) virus binds and sequesters plasminogen on the cell surface, leading to enhanced cleavage of the viral hemagglutinin...
  86. pmc Budding capability of the influenza virus neuraminidase can be modulated by tetherin
    Mark A Yondola
    Mount Sinai School of Medicine, 1 Gustave L Levy Place, Department of Microbiology, Annenberg Bldg 16 20, New York, NY 10128, USA
    J Virol 85:2480-91. 2011
    We have determined that, in addition to its receptor-destroying activity, the influenza virus neuraminidase is capable of efficiently forming virus-like particles (VLPs) when expressed individually from plasmid DNA...
  87. pmc Influenza virus neuraminidase contributes to secondary bacterial pneumonia
    Ville T Peltola
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    J Infect Dis 192:249-57. 2005
    ..The specific level of their neuraminidase activity correlated with their ability to support secondary bacterial pneumonia...
  88. pmc Glycosylation site alteration in the evolution of influenza A (H1N1) viruses
    Shisheng Sun
    Laboratory of Functional Glycomics, College of Life Sciences, National Engineering Research Center for Miniaturized Detection System, Northwest University, Xi an, People s Republic of China
    PLoS ONE 6:e22844. 2011
    ....
  89. pmc Hemagglutinin-neuraminidase balance confers respiratory-droplet transmissibility of the pandemic H1N1 influenza virus in ferrets
    Hui Ling Yen
    Department of Microbiology, HKU Pasteur Research Centre, University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Proc Natl Acad Sci U S A 108:14264-9. 2011
    ..the M gene derived from EAsw and differed from the gene constellation of the pandemic H1N1 virus by the neuraminidase (NA) gene alone...
  90. ncbi Evaluation of hemagglutinin subtype 1 swine influenza viruses from the United States
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, ARS, Ames, IA 50010, USA
    Vet Microbiol 118:212-22. 2006
    ..In addition, the hemagglutinin and neuraminidase genes of each isolate were sequenced for genetic comparison, and serological cross-reactivity was evaluated ..
  91. pmc Overexpression of the alpha-2,6-sialyltransferase in MDCK cells increases influenza virus sensitivity to neuraminidase inhibitors
    Mikhail Matrosovich
    Institute of Virology, Philipps University, 35037 Marburg, Germany
    J Virol 77:8418-25. 2003
    No reliable cell culture assay is currently available for monitoring human influenza virus sensitivity to neuraminidase inhibitors (NAI)...
  92. pmc Improvement of influenza A/Fujian/411/02 (H3N2) virus growth in embryonated chicken eggs by balancing the hemagglutinin and neuraminidase activities, using reverse genetics
    Bin Lu
    MedImmune Vaccines, Inc, 297 N Bernardo Ave, Mountain View, CA 94043, USA
    J Virol 79:6763-71. 2005
    ..A/Sendai differed from A/Fujian by three amino acids in the neuraminidase (NA), whereas A/Wyoming differed from A/Fujian by five amino acids in the hemagglutinin (HA)...
  93. pmc CD8+ T-Cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes
    Diana L Martin
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, United States of America
    PLoS Pathog 2:e77. 2006
    ..Experimental models also demonstrated that the dominance patterns vary depending on the infective strain of T. cruzi, suggesting that immune evasion may be occurring at a population rather than single-parasite level...
  94. ncbi Infections with oseltamivir-resistant influenza A(H1N1) virus in the United States
    Nila J Dharan
    Epidemic Intelligence Service, Office of Workforce and Career Development Assigned to Influenza Division, Centers for Disease Control and Prevention, 1600 Clifton Rd, MS A 32, Atlanta, GA 30333, USA
    JAMA 301:1034-41. 2009
    ..Early surveillance data suggest that the prevalence of oseltamivir resistance among A(H1N1) viruses will most likely be higher during the 2008-2009 season...
  95. ncbi Structural basis for oseltamivir resistance of influenza viruses
    P J Collins
    MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Vaccine 27:6317-23. 2009
    Oseltamivir, one of the two anti-neuraminidase drugs, is currently the most widely used drug against influenza...
  96. ncbi Emergence of H274Y oseltamivir-resistant A(H1N1) influenza viruses in Japan during the 2008-2009 season
    Tatiana Baranovich
    Division of Public Health, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1 757 Asahimachi dori, Chuoku, Niigata City, Niigata Prefecture 951 8510, Japan
    J Clin Virol 47:23-8. 2010
    ..A substantial increase in oseltamivir-resistant A(H1N1) influenza viruses was reported in Europe in late 2007...
  97. ncbi Critical role for cross-linking of trimeric lectin domains of surfactant protein D in antiviral activity against influenza A virus
    Tesfaldet Tecle
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Biochem J 412:323-9. 2008
    ..mAbs significantly increased binding and antiviral activity of NCRDs as assessed by haemagglutination and neuraminidase inhibition and by viral neutralization...
  98. ncbi Contacts between influenza virus N9 neuraminidase and monoclonal antibody NC10
    Janis T Lee
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    Virology 300:255-68. 2002
    ..We propose that the difference lies in the contribution of buried water molecules to the NA-NC10 complex...
  99. pmc Transport of human lysosomal neuraminidase to mature lysosomes requires protective protein/cathepsin A
    A Van der Spoel
    Department of Genetics, St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, TN 38105, USA
    EMBO J 17:1588-97. 1998
    Human lysosomal N-acetyl-alpha-neuraminidase is deficient in two lysosomal storage disorders, sialidosis, caused by structural mutations in the neuraminidase gene, and galactosialidosis, in which a primary defect of protective protein/..
  100. pmc Influenza A virus neuraminidase limits viral superinfection
    I Chueh Huang
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Southborough, Massachusetts 01772, USA
    J Virol 82:4834-43. 2008
    ..Here, we show that cellular expression of influenza A virus neuraminidase (NA), but not hemagglutinin (HA) or the M2 proton pump, inhibits entry of HA-pseudotyped retroviruses...
  101. pmc Distinct kinetics of effector CD8+ cytotoxic T cells after infection with Trypanosoma cruzi in naive or vaccinated mice
    Fanny Tzelepis
    CINTERGEN, UNIFESP Escola Paulista de Medicina, Rua Mirassol, 207, Sao Paulo SP, Brazil, 04044 010
    Infect Immun 74:2477-81. 2006
    ..In contrast, in vaccinated mice, the swift CD8+-T-cell response helps host survival after challenge...

Research Grants65

  1. Carbohydrate-based Antiinfective Agents
    Chi Huey Wong; Fiscal Year: 2013
    ..in two aims targeting: 1) bacterial transglycosylase, and 2) the influenza coat proteins hemagglutinin and neuraminidase. Transglycosylase (TGase) is the enzyme responsible for assembling the carbohydrate backbone of the bacterial ..
  2. H. influenzae: Role of Sialic Acid in Virulence
    Michael A Apicella; Fiscal Year: 2013
    ..13 annotated NTHi genomes indicate that there are no genes homologous to any known eukaryotic or prokaryotic neuraminidase. We have also found that about 40% of NTHi strains do not incorporate sialic acid into their LOS...
  3. Crossprotective CTL Against Influenza
    Ellis L Reinherz; Fiscal Year: 2013
    ..based upon elicitation of neutralizing antibodies directed at virion surface expressed hemagglutinin (HA) and neuraminidase (NA) proteins...
  4. H. influenzae: Role of Sialic Acid in Virulence
    Michael Apicella; Fiscal Year: 2009
    ..13 annotated NTHi genomes indicate that there are no genes homologous to any known eukaryotic or prokaryotic neuraminidase. We have also found that about 40% of NTHi strains do not incorporate sialic acid into their LOS...
  5. The Evolutionary and Biological Bases of Host Switching in Viruses
    Colin R Parrish; Fiscal Year: 2013
    ..the evolutionary connections between the host sialic acid receptors and the viral hemagglutinin and neuraminidase genes...
  6. A novel assay for inhibitors of influenza A virus polymerase complex assembly
    Feng Li; Fiscal Year: 2009
    Although effective neuraminidase inhibitors are available to treat influenza viral infections in humans, the emergence of virus isolates resistant to these drugs can have a significant adverse impact on both treatment options and ..
  7. Leukocyte Signaling in the Elderly and Vaccine Immunogenicity
    DAVID BRAM LEWIS; Fiscal Year: 2012
    ..TIV, which contains the hemagglutinin (HA) and neuraminidase (NA) major viral surface proteins and is unadjuvanted, provides protection mainly by inducing neutralizing ..
  8. Surrogate endpoints for correlating protective immunity in response to influenza
    Gary Fujii; Fiscal Year: 2010
    ..ectodomain segment of the Matrix 2 (M2e) protein as an alternative to the haemagglutinin (H or HA) or neuraminidase (N) antigens for vaccine development...
  9. Adjuvant Enhanced Antiviral Immunity
    JEFF C FAIRMAN; Fiscal Year: 2011
    ..The efficacy of these vaccines is highly dependent on close matching of the hemagglutinin (HA) and neuraminidase (NA) surface proteins of the vaccine with currently circulating virus...
  10. ROLE OF COMPLEMENT RECEPTOR 1 IN ERYTHROCYTE INVASION BY PLASMODIUM FALCIPARUM IN
    GORDON AKANZUWINE AWANDARE; Fiscal Year: 2013
    ..However, some P. falciparum strains have the ability to invade neuraminidase-treated erythrocytes which lack SA, demonstrating the existence of alternative SA-independent invasion ..
  11. TRANSMISSION OF OSELTAMIVIR-RESISTANT INFLUENZA A H1N1 VIRUSES IN GUINEA PIGS
    Nicole M Bouvier; Fiscal Year: 2012
    ..viruses were less fit than sensitive strains, due to compromised enzymatic activity of the mutated neuraminidase (NA) [18, 48-50, 70, 71]...
  12. Eritoran (E5564), a TLR4 antagonist, as a novel therapeutic for influenza
    Stefanie N Vogel; Fiscal Year: 2013
    ..Antiviral agents, such as neuraminidase inhibitors (NAIs), are hampered by the increasing resistance of many influenza strains to such agents and the ..
  13. 3D human lung model to study lung disease and formation of fibrosis
    Joan E Nichols; Fiscal Year: 2013
    ..strains of influenza A that do not elicit these responses will be used as controls as will addition of neuraminidase inhibitors Tamiflu and Amantidine...
  14. Structural insights into pandemic and emerging influenza viruses
    Ian A Wilson; Fiscal Year: 2013
    ..Influenza A viruses exhibit extreme diversity via multiple serotypes of the hemagglutinin (HA 1-16) and neuraminidase (NA 1-9) surface antigens...
  15. Investigating the Homing and Engraftment of CML Stem Cells in the Bone Marrow
    JULIANA BETH LEWIS; Fiscal Year: 2012
    ..b>Neuraminidase, anti-selectin reagents, monoclonal antibodies and small molecule selectin antagonists will be tested for ..
  16. Development and Maintenance of Memory CD8 T Cells
    Jianzhu Chen; Fiscal Year: 2010
    ..every year due to waning of the elicited antibody responses to viral coat proteins hemagglutanin (HA) and neuraminidase (NA) in about six months and rapid changes of viral HA and NA genes...
  17. The Role of the Actin Cytoskeleton and Cholesterol in Influenza Virus Assembly
    Samuel T Hess; Fiscal Year: 2010
    ..through hemagglutinin-mediated fusion, and for assembly of viral components such as hemagglutinin (HA) and neuraminidase (NA) before budding...
  18. Molecular basis for paramyxovirus entry
    Anne Moscona; Fiscal Year: 2013
    ..During entry, the viral surface glycoproteins HN (receptor-binding protein;hemagglutinin-neuraminidase) and F (fusion protein) cooperate to mediate fusion upon receptor binding...
  19. Molecular basis for paramyxovirus entry
    Anne Moscona; Fiscal Year: 2012
    ..During entry, the viral surface glycoproteins HN (receptor-binding protein;hemagglutinin-neuraminidase) and F (fusion protein) cooperate to mediate fusion upon receptor binding...
  20. Hepatic Lectin Receptors in Glycoprotein Homeostasis
    JAMEY MARTH; Fiscal Year: 2013
    ..AMR), binds and internalizes exogenously administered circulating glycoproteins that have been remodeled by neuraminidase (sialidase) activity...
  21. Foundation Immunogens for Influenza Vaccines
    Eric A Weaver; Fiscal Year: 2013
    ..These will include hemagglutinin (HA) and neuraminidase (NA) for H1, H2, H3, H5, N1 and N2 subtypes...
  22. Structure of M2 proton channel from influenza A Virus
    William F Degrado; Fiscal Year: 2013
    ..and rimantadine target the M2 proton channel, while Tamiflu (oseltamivir) and related compounds target neuraminidase. Resistance to both classes of drugs poses a major problem, and most isolates of influenza A virus are now ..
  23. FUNCTIONAL/STRUCTURAL STUDIES OF MAMMALIAN NEURAMINIDASE
    ALESSANDRA D AZZO; Fiscal Year: 2009
    The scope of this proposal is to study the function of mammalian lysosomal neuraminidase (NEU1) in normal cell metabolism, and the consequences of its loss in human diseases. NEU1 belongs to the ubiquitous superfamily of sialidases...
  24. Identification of Novel Broad Spectrum Influenza Virus Inhibitors
    Arnab Basu; Fiscal Year: 2013
    ..The inhibitors also displayed synergy with the neuraminidase inhibitor, oseltamivir...
  25. Identification of novel influenza antivirals
    GREGORY WILLIAM HENKEL; Fiscal Year: 2012
    ..However, only one class of drug (neuraminidase inhibitors) is currently available;amantadine-resistance has become so widespread the amantadanes have become ..
  26. Influenza vaccines inducing broadly cross protective immunity
    Sang Moo Kang; Fiscal Year: 2013
    ..Current influenza vaccines are targeted to induce immune responses to the variable antigens hemagglutinin and neuraminidase, which are effective for strain-specific protection...
  27. Development of drugs that target the M2 proton channel from the influenza A Virus
    William F Degrado; Fiscal Year: 2012
    ..viral proteins: amantadine targets a protein called the M2 proton channel, while Tamiflu (oseltamivir) targets neuraminidase. In the last flu season, most strains of the influenza virus (including H5N1) have become resistant to the ..
  28. Constraints on the evolution of influenza oseltamivir resistance
    Jesse D Bloom; Fiscal Year: 2012
    ..of oseltamivir (Tamiflu) resistance in H1N1 influenza viruses via the His274->Tyr (H274Y) mutation to the neuraminidase protein...
  29. Novel role of 1918 influenza virus neuraminidase in hemagglutnin activation
    DONNA TSCHERNE; Fiscal Year: 2010
    ..RNA genome contained within an envelope that is decorated with 2 surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA)...
  30. Role of CCR1 in Cytokine Storm &Immunopathology after influenza infection
    Troy D Randall; Fiscal Year: 2013
    ..Antiviral neuraminidase inhibitors are able to control viral infection if given early to patients, but no drugs have been reported to ..
  31. Gene Transfer for Galactosialidosis - Resub
    Arthur W Nienhuis; Fiscal Year: 2013
    ..protein/cathepsin A, that results in the secondary combined deficiency of [unreadable]- galactosidase and neuraminidase. The initial participants targeted for gene transfer will be individuals with the late, infantile phenotype in ..
  32. Nanoemulsions as novel adjuvents for influenza virus vaccines
    Yoshihiro Kawaoka; Fiscal Year: 2011
    ..In Aim 1, we plan to establish neuraminidase-deficient viruses as live attenuated influenza vaccines...
  33. Modulation of drug metabolism by Danshen
    Philip M Potter; Fiscal Year: 2013
    ..drug oseltamivir phosphate (Tamiflu) that requires hydrolysis to the carboxylate form to yield the active neuraminidase inhibitor...
  34. Scalable tools for the analysis of chemical compounds using graph-based querying
    Christian Lang; Fiscal Year: 2010
    ..3) apply our tool set to specific relevant research problems such as HIV-1 Protease inhibition, Avian Flu neuraminidase inhibition, and p53-protein interactions...
  35. Mechanisms of Pneumococcal Adherence
    SAMANTHA JANE KING; Fiscal Year: 2012
    ..We have identified a novel exoglycosidase-dependent adherence mechanism requiring the S.p. neuraminidase, NanA, and 2- galactosidase, BgaA. This mechanism is relevant to adherence of recent clinical isolates and S.p...
  36. Receptor Mimics for Rapid Detection, Typing, and Susceptibility Testing of Influe
    Alison A Weiss; Fiscal Year: 2013
    ..to develop novel glycan based diagnostic platforms that target the viral glycoproteins, Hemagglutinin (HA) and Neuraminidase (NA), which are abundant (~50 copies of tetrameric NA and 300 copies of trimeric HA) on the surface of the ..
  37. SIMULTANEOUS SCREENING FOR A/H3N2, A/H1N1, A/H5N1 AND B INFLUENZA VIRUSES
    Erica Dawson; Fiscal Year: 2009
    ..labs rely on a fluorescence-based immunoassay to screen for H3 or H1 viruses, with no confirmation of the neuraminidase subtype and no current capabilities for emerging viruses...
  38. ACROSOMAL ENZYMES AND THEIR ROLE IN FERTILIZATION
    PRAKASH SRIVASTAVA; Fiscal Year: 1993
    ..After the extraction of acrosomal enzymes, sperm still contain nearly one-half of neuraminidase, one third of arylsulfatase, and one third of hyaluronidase...
  39. NMR-based discovery of influenza hemagglutinin probes
    Michael S Caffrey; Fiscal Year: 2013
    ..Current treatments include Tamiflu (oseltamivir) and Relenza (zanamivir), which target the membrane protein neuraminidase (NA). Unfortunately, resistance is increasing in circulating influenza strains...
  40. Prodrugs of Neuraminidase Inhibitors for Increased Oral Bioavailability
    John M Hilfinger; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Virally-encoded neuraminidase plays a key role in the life-cycle of the influenza virus...
  41. REGULATION OF PANCREATIC MUSCARINIC RECEPTORS IN VITRO
    SETH HOOTMAN; Fiscal Year: 1990
    ..After labelling, plasma membranes will be prepared and treated with neuraminidase, endoglycosidase F, and proteases and then solubilized and electrophoresed...
  42. INHIBITION OF INFLUENZA VIRUS NS1 FUNCTION BY SMALL MOLECULES
    MEGAN LOUISE SHAW; Fiscal Year: 2010
    ..Widespread resistance to the M2 inhibitors, and most recently to the neuraminidase inhibitors as well, makes it imperative that we develop new and improved antiviral drugs against influenza ..
  43. OPTIMIZATION OF NOVEL INFLUENZA M2 CHANNEL INHIBITORS
    KEN J MCCORMACK; Fiscal Year: 2013
    ..Significant resistance to neuraminidase inhibitors has also been observed in some influenza A strains...
  44. ENZYMIC AND NON-ENZYMIC GLYCOSYL TRANSFER
    Michael Sinnott; Fiscal Year: 1993
    ..Labelled NANA derivatives will be used to probe the mechanism of neuraminidase, to see if the carboxylate group of the substrate takes the place of the enzyme carboxylate group which acts ..
  45. Development of novel broad-spectrum influenza A inhibitors
    KEN J MCCORMACK; Fiscal Year: 2012
    ..have become all but ineffective and some flu strains have already exhibited significant resistance to neuraminidase inhibitors...
  46. STREPTOCOCCUS PNEUMONIAE ADHERENCE AND OTITIS MEDIA PATH
    Thomas Demaria; Fiscal Year: 2004
    ..The specific aims of this proposal are: 1) To continue to define the role of Spn neuraminidase in the pathogenesis of OM and to assess whether it is a protective antigen and a potential protein-based ..
  47. Genetic System to Study Virulence in Bacteroides
    Michael Malamy; Fiscal Year: 2003
    ..We will focus on the operon containing the neuraminidase (nanH1) gene and several other glycohydrolases capable of converting the complex Lewis antigen found on the ..
  48. Portable Multiplexed LATE-PCR Influenza Test
    Barry E Boyes; Fiscal Year: 2010
    ..of highly pathogenic varieties), reduce unneeded antibiotic use, reduce hospital stays, and enable more effective use of neuraminidase inhibitors, preserving Strategic National Stockpile of drugs for response to pandemic influenza.
  49. IMMUNE RESPONSES TO CLYCOSYLATION-MODIFIED SIV VACCINES
    David Montefiori; Fiscal Year: 1993
    ..I inhibitor) and swainsonine (mannosidase II inhibitor) or by enzymatic removal of sialic acids using neuraminidase. Three different molecular species of high mannose-type, non-sialylated, non-fucosylated carbohydrate moieties ..
  50. New Influenza A Neuraminidase Inhibitors for Biodefense
    Wayne Brouillette; Fiscal Year: 2009
    ..One such drug target is the neuraminidase (NA)...
  51. ALPHA-1-PROTEASE INHIBITOR DEFICIENCY--GENE THERAPY
    Jerry Brown; Fiscal Year: 1991
    ..protein to contain appropriate ligands for interaction with the ASGP receptor: 1) treatment of MoMLV with neuraminidase to expose terminal galactose residues, 2) reductive lactosamination of MoMLV to increase the number of ..
  52. Competition Among Bacterial Resiratory Pathogens
    Jeffrey Weiser; Fiscal Year: 2004
    ..In addition, a neuraminidase expressed by S. pneumoniae can desialylate host receptors and the sialylated LPS of H. influenzae...
  53. Alphavirus Replicon Vaccines against Influenza
    Robert Olmsted; Fiscal Year: 2005
    ..encephalitis (VEE) virus, to produce virus-like replicon particles (VRP) expressing the hemagglutinin (HA) and neuraminidase (NA) genes of influenza A. Preliminary studies have demonstrated the safety and feasibility of this approach...
  54. Luciferase-encoded influenza viruses for antiviral screening
    DEAN KEDES; Fiscal Year: 2006
    ..emergence of drug resistant mutants, including those dually resistant to existing drugs M2-blockers and neuraminidase (NA) inhibitors has been reported...
  55. MECHANISM OF NANAASE-ENHANCED TUMOR IMMUNOGENICITY
    JACK PINCUS; Fiscal Year: 1980
    Immunization of tumor-bearing hosts with Vibrio cholerae neuraminidase (NANAase)-treated tumor cells alone or in combination with chemotherapy slows the growth or causes regression of established tumors...
  56. DESIGN OF INFLUENZA VIRUS NEURAMINIDASE INHIBITORS
    Wayne Brouillette; Fiscal Year: 1999
    ..New drugs which the virus cannot easily evade by mutation are needed. Neuraminidase (NA) is a surface enzyme on influenza A and B viruses which is required for release of progeny virus...
  57. Fusion triggering by Hendra virus F protein: role of G
    Anne Moscona; Fiscal Year: 2004
    ..For HPF3, our central hypothesis is that the interaction of the receptor-binding protein, hemagglutinin-neuraminidase (HN), with cellular receptor(s) is critical for several essential components of the viral life cycle -- ..
  58. CHAGAS' DISEASE THERAPY: INHIBITORS OF TRYPANOSOMA CRUZI
    MARINA CHUENKOVA; Fiscal Year: 2000
    ..b>Neuraminidase/trans-sialidase (TSA) is a T...
  59. Lipid/DNA/Antigen Complexes for Influenza A Viral Vaccination
    Jeff Fairman; Fiscal Year: 2007
    ..The efficacy of these vaccines is highly dependent on close matching of the hemagglutinin (HA) and neuraminidase (NA) surface proteins of the vaccine with currently circulating virus...
  60. INTERPLAY BETWEEN THE HA AND NA OF INFLUENZA VIRUSES
    Larisa Gubareva; Fiscal Year: 2003
    ..The two major surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA), play important roles in tissue and organ tropism, spread, and pathogenicity of influenza viruses...
  61. Public Health Strategies for the Next Influenza Pandemic
    Nayer Khazeni; Fiscal Year: 2009
    ..Examining the long-term safety and effectiveness of extended prophylaxis with neuraminidase inhibitors by performing a systematic review and meta-analysis. 2...
  62. NEW APPROACHES TO THE CONTROL OF INFLUENZA
    Robert Webster; Fiscal Year: 1999
    ..knowledge gained during the current grant period on the three-dimensional (3D) structure of an influenza virus neuraminidase antibody complex and with the available structural information in the uncomplexed hemagglutinin and ..
  63. ANALYSIS OF PARAINFLUENZA 3 VIRUS MEDIATED FUSION
    Mark Galinski; Fiscal Year: 1991
    ..The PF3 contains two surface glycoproteins, HN, which has hemagglutinating and neuraminidase activity, and F, which has fusion activity...
  64. EFFICACY OF OSELTAMIVIR IN REDUCING DURATION OF ILLNESS, VIRAL SHEDDING & TRANSIS
    W Abdullah Brooks; Fiscal Year: 2010
    ..all treatment groups vs placebo, risk of transmission to household contacts by treatment group and whether neuraminidase inhibitor use creates resistance. Secondarily it aims to measure the effect on influenza...
  65. Adaptation of Influenza Inhibition Assays for High-throughput Screening
    James Noah; Fiscal Year: 2006
    ..additional antiviral compounds effective against four validated, high-priority biochemical targets: 1) neuraminidase, 2) NS1, 3) polymerase cap snatching activity and 4) polymerase endonuclease activity...