- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis
Jesper V Olsen
Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
Sci Signal 3:ra3. 2010
..In particular, nuclear proteins and proteins involved in regulating metabolic processes have high phosphorylation site occupancy in mitosis. This suggests that these proteins may be inactivated by phosphorylation in mitotic cells...
- The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP
Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Cell 122:421-34. 2005
..Thus, Yki is a critical target of the Wts/Lats protein kinase and a potential oncogene...
- p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 398:714-8. 1999
..Taken together, our results indicate that p63 is critical for maintaining the progenitor-cell populations that are necessary to sustain epithelial development and morphogenesis...
- TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling
Department of Biological Chemistry, University of Michigan Medical School, 1301 Catherine Road, Ann Arbor, MI 48109, USA
Nat Cell Biol 4:648-57. 2002
..Our data indicate a molecular mechanism for TSC2 in insulin signalling, tumour suppressor functions and in the inhibition of cell growth...
- p63 is a p53 homologue required for limb and epidermal morphogenesis
A A Mills
Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Nature 398:708-13. 1999
..Thus, in contrast to p53, p63 is essential for several aspects of ectodermal differentiation during embryogenesis...
- p63 Is essential for the proliferative potential of stem cells in stratified epithelia
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Cell 129:523-36. 2007
..Together, these data identify p63 as a key, lineage-specific determinant of the proliferative capacity in stem cells of stratified epithelia...
- IFI16 is an innate immune sensor for intracellular DNA
School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
Nat Immunol 11:997-1004. 2010
..IFI16 (p204) is the first PYHIN protein to our knowledge shown to be involved in IFN-β induction. Thus, the PYHIN proteins IFI16 and AIM2 form a new family of innate DNA sensors we call 'AIM2-like receptors' (ALRs)...
- Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
Research Institute of Molecular Pathology, Vienna, Austria
Cell 91:35-45. 1997
..Proteins homologous to Scc1p exist in a variety of eukaryotic organisms including humans. A common cohesion apparatus might be used by all eukaryotic cells during both mitosis and meiosis...
- Occludin: a novel integral membrane protein localizing at tight junctions
Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
J Cell Biol 123:1777-88. 1993
..These findings revealed that an integral membrane protein localizing at tight junctions is now identified, which we designated as "occludin."..
- TEAD mediates YAP-dependent gene induction and growth control
Department of Pharmacology and Moores Cancer Center, University of California at San Diego, La Jolla, California 92093, USA
Genes Dev 22:1962-71. 2008
..Our study reveals TEAD as a new component in the Hippo pathway playing essential roles in mediating biological functions of YAP...
- Phospho.ELM: a database of phosphorylation sites--update 2011
SCB Unit, EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
Nucleic Acids Res 39:D261-7. 2011
..Finally, special emphasis has been put on linking to external resources such as interaction networks and other databases...
- Identification and validation of oncogenes in liver cancer using an integrative oncogenomic approach
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Cell 125:1253-67. 2006
..Our results establish a tractable model of liver cancer, identify two oncogenes that cooperate by virtue of their coamplification in the same genomic locus, and suggest an efficient strategy for the annotation of human cancer genes...
- Transforming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 103:12405-10. 2006
- Akt phosphorylates the Yes-associated protein, YAP, to induce interaction with 14-3-3 and attenuation of p73-mediated apoptosis
Signal Transduction, Cancer Research UK London Research Institue, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
Mol Cell 11:11-23. 2003
..Akt phosphorylation of YAP may thus suppress the induction of the proapoptotic gene expression response following cellular damage...
- Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis
Dawn L Brasaemle
Department of Nutritional Sciences and the Rutgers Center for Lipid Research, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA
J Lipid Res 48:2547-59. 2007
..A model is discussed whereby perilipin serves as a dynamic scaffold to coordinate the access of enzymes to the lipid droplet in a manner that is responsive to the metabolic status of the adipocyte...
- Trex1 prevents cell-intrinsic initiation of autoimmunity
Daniel B Stetson
Howard Hughes Medical Institute and Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
Cell 134:587-98. 2008
..These findings reveal a cell-intrinsic mechanism for initiation of autoimmunity, implicate the ISD pathway as the cause of AGS, and suggest an unanticipated contribution of endogenous retroelements to autoimmunity...
- YAP1 increases organ size and expands undifferentiated progenitor cells
Fernando D Camargo
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
Curr Biol 17:2054-60. 2007
..Our data show that the Hippo signaling pathway regulates organ size in mammals and can act on stem cell compartments, indicating a potential link between stem/progenitor cells, organ size, and cancer...
- Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements
Michael T Veeman
Department of Pharmacology, Center for Developmental Biology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA 98195, USA
Curr Biol 13:680-5. 2003
..Our results support the idea that a vertebrate PCP pathway regulates gastrulation movements and suggest that there is overlap between the PCP and Wnt/calcium pathways...
- BAR domains as sensors of membrane curvature: the amphiphysin BAR structure
Brian J Peter
Medical Research Council MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
Science 303:495-9. 2004
..From this, we predict that BAR domains are in many protein families, including sorting nexins, centaurins, and oligophrenins. The universal and minimal BAR domain is a dimerization, membrane-binding, and curvature-sensing module...
- The role of YAP transcription coactivator in regulating stem cell self-renewal and differentiation
Moores Cancer Center, University of California at San Diego, La Jolla, California 92093, USA
Genes Dev 24:1106-18. 2010
..Moreover, YAP binds directly to promoters of a large number of genes known to be important for stem cells and stimulates their expression. Our observations establish a critical role of YAP in maintaining stem cell pluripotency...
- The Hippo pathway target, YAP, promotes metastasis through its TEAD-interaction domain
John M Lamar
Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 109:E2441-50. 2012
..Together, our results suggest that increased YAP/TEAD activity plays a causal role in cancer progression and metastasis...
- Yap1 acts downstream of α-catenin to control epidermal proliferation
Stem Cell Program, Children s Hospital, Boston, MA 02115, USA
Cell 144:782-95. 2011
..Together, these data identify Yap1 as a determinant of the proliferative capacity of epidermal stem cells and as an important effector of a "crowd control" molecular circuitry in mammalian skin...
- Dishevelled: The hub of Wnt signaling
Tsinghua University, Beijing, China
Cell Signal 22:717-27. 2010
..In this review, we summarize the current understanding of Dvl functions in Wnt signal transduction and its biological functions in mouse development, and also discuss the molecular mechanisms of its actions...
- Wnt signaling and cancer
Department of Molecular Oncology, Genentech Inc, South San Francisco, California 94080 USA
Genes Dev 14:1837-51. 2000
- Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions
Department of Cell Biology, University of Connecticut Health Center, Farmington, 06030, USA
Sci Signal 2:ra46. 2009
..We propose that modulation of PPIs by stimulus-dependent changes in S-T phosphorylation state is a widespread phenomenon applicable to many other signaling systems...
- PHOSIDA (phosphorylation site database): management, structural and evolutionary investigation, and prediction of phosphosites
Department for Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz, D 82152 Martinsried, Germany
Genome Biol 8:R250. 2007
..For each phosphosite, PHOSIDA lists matching kinase motifs, predicted secondary structures, conservation patterns, and its dynamic regulation upon stimulus. Using support vector machines, PHOSIDA also predicts phosphosites...
- Identification of nucleolin as a cellular receptor for human respiratory syncytial virus
The James Hogg Research Centre, Providence Heart and Lung Institute at St Paul s Hospital, Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada
Nat Med 17:1132-5. 2011
..RNAi-mediated knockdown of lung nucleolin was associated with a significant reduction in RSV infection in mice (P = 0.0004), confirming that nucleolin is a functional RSV receptor in vivo...
- Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle
Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Mol Cell 31:438-48. 2008
..These results provide a vastly extended knowledge base for functional studies on kinases and their regulation through site-specific phosphorylation...
- The substrate of Greatwall kinase, Arpp19, controls mitosis by inhibiting protein phosphatase 2A
Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
Science 330:1673-7. 2010
..Although both proteins can inhibit PP2A, endogenous Arpp19, but not α-Endosulfine, is responsible for PP2A inhibition at mitotic entry in Xenopus egg extracts...
- Greatwall phosphorylates an inhibitor of protein phosphatase 2A that is essential for mitosis
Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
Science 330:1670-3. 2010
..This converts Ensa into a potent and specific inhibitor of PP2A-B55δ. This pathway represents a previously unknown element in the control of mitosis...
- p63 is the molecular switch for initiation of an epithelial stratification program
Maranke I Koster
Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
Genes Dev 18:126-31. 2004
..Our data demonstrate that p63 plays a dual role: initiating epithelial stratification during development and maintaining proliferative potential of basal keratinocytes in mature epidermis...
- Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis
Joris J Benschop
Molecular Genetics, Utrecht University, Padualaan 8, 3584CH Utrecht, The Netherlands
Mol Cell Proteomics 6:1198-214. 2007
..Our study presents the largest quantitative Arabidopsis phosphoproteomics data set to date and provides a new resource that can be used to gain novel insight into plant defense signal transduction and early defense response...
- Kibra functions as a tumor suppressor protein that regulates Hippo signaling in conjunction with Merlin and Expanded
Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA
Dev Cell 18:288-99. 2010
..These results shed light on the mechanism of Ex and Mer function and implicate Kibra as a potential tumor suppressor with relevance to neurofibromatosis...
- PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores
Perry E Bickel
Center for Diabetes and Obesity Research, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas, USA
Biochim Biophys Acta 1791:419-40. 2009
..In this review, we discuss how the PAT proteins regulate cellular lipid metabolism both in mammals and in model organisms...
- Phosphoproteomic analysis reveals interconnected system-wide responses to perturbations of kinases and phosphatases in yeast
Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland
Sci Signal 3:rs4. 2010
..cerevisiae) to determine the relationships between 97 kinases, 27 phosphatases, and more than 1000 phosphoproteins. We identified 8814 regulated phosphorylation events, describing the first system-wide protein ..
- mTORC1-mediated cell proliferation, but not cell growth, controlled by the 4E-BPs
Ryan J O Dowling
Department of Biochemistry and Goodman Cancer Research Centre, McGill University, Montreal, Quebec H3A 1A3, Canada
Science 328:1172-6. 2010
..Thus, control of cell size and cell cycle progression appear to be independent in mammalian cells, whereas in lower eukaryotes, 4E-BPs influence both cell growth and proliferation...
- PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
Mol Cell 25:903-15. 2007
..We propose that the relative strengths of the rheb- and PRAS40-mediated inputs to mTORC1 set overall pathway activity and that insulin activates mTORC1 through the coordinated regulation of both...
- Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
J Cell Biol 140:647-57. 1998
- Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40
Emilie Vander Haar
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 6 155 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA
Nat Cell Biol 9:316-23. 2007
..These findings identify PRAS40 as an important regulator of insulin sensitivity of the Akt-mTOR pathway and a potential target for the treatment of cancers, insulin resistance and hamartoma syndromes...
- ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation
Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Kyoto 606 8501, Japan
Cell 126:741-54. 2006
..When NZO-1 was forcibly recruited to lateral membranes and dimerized, claudins were dramatically polymerized. These findings indicate that ZO-1 and ZO-2 can independently determine whether and where claudins are polymerized...
- Wnt induces LRP6 signalosomes and promotes dishevelled-dependent LRP6 phosphorylation
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Science 316:1619-22. 2007
..We propose that Wnts induce coclustering of receptors and Dvl in LRP6-signalosomes, which in turn triggers LRP6 phosphorylation to promote Axin recruitment and beta-catenin stabilization...
- Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS
Scott A Gerber
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 100:6940-5. 2003
..The methods described here represent focused, alternative approaches for studying the dynamically changing proteome...
- TAp63 induces senescence and suppresses tumorigenesis in vivo
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
Nat Cell Biol 11:1451-7. 2009
..The ability of TAp63 to trigger senescence and halt tumorigenesis irrespective of p53 status identifies TAp63 as a potential target of anti-cancer therapy for human malignancies with compromised p53...
- A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae
Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
Cell 91:47-57. 1997
..These data implicate Mcd1p as a nexus between cohesion and condensation. We present a model for mitotic chromosome structure that incorporates this previously unsuspected link...
- 53BP1, a mediator of the DNA damage checkpoint
Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Science 298:1435-8. 2002
- Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function
Department of Biochemistry, McGill University, Montreal, Quebec, Canada
Nature 371:762-7. 1994
..The action of this new regulator of protein synthesis is therefore modulated by insulin, which acts to stimulate the overall rate of translation and promote cell growth...
- Paxillin: adapting to change
Michael C Brown
Dept of Cell and Developmental Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
Physiol Rev 84:1315-39. 2004
- 4E-BP1 is a key effector of the oncogenic activation of the AKT and ERK signaling pathways that integrates their function in tumors
Qing Bai She
Program in Molecular Pharmacology and Chemistry, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
Cancer Cell 18:39-51. 2010
..Thus, 4E-BP1 plays a prominent role in mediating the effects of these pathways in tumors in which they are activated by mutation...
- A multidimensional chromatography technology for in-depth phosphoproteome analysis
Claudio P Albuquerque
Ludwig Institute for Cancer Research, Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA
Mol Cell Proteomics 7:1389-96. 2008
..Saccharomyces cerevisiae after DNA damage led to the identification of 8764 unique phosphopeptides from 2278 phosphoproteins using tandem MS...
- RASSF1A elicits apoptosis through an MST2 pathway directing proapoptotic transcription by the p73 tumor suppressor protein
Signaling and Proteomics Laboratory, The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
Mol Cell 27:962-75. 2007
..Our results describe an MST2-dependent effector pathway for RASSF1A proapoptotic signaling and indicate that silencing of RASSF1A in tumors removes a proapoptotic signal emanating from p73...
- A coordinated phosphorylation by Lats and CK1 regulates YAP stability through SCF(beta-TRCP)
Department of Pharmacology and Moores Cancer Center, University of California at San Diego, La Jolla, California 92093 0815, USA
Genes Dev 24:72-85. 2010
- SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity
Department of Physiology and Biophysics, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Cell 127:125-37. 2006
..Our findings reveal that the SIN1-rictor-mTOR function in Akt-Ser473 phosphorylation is required for TORC2 function in cell survival but is dispensable for TORC1 function...
- Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins
Alan R Kimmel
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
J Lipid Res 51:468-71. 2010
..Each gene member will incorporate the root term PERILIPIN (PLIN), the founding gene of the PAT family, with the different genes/proteins numbered sequentially...
- Shugoshin collaborates with protein phosphatase 2A to protect cohesin
Tomoya S Kitajima
Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
Nature 441:46-52. 2006
..Thus, we have revealed a conserved mechanism of centromeric protection of eukaryotic chromosomes in mitosis and meiosis...
- A core function for p120-catenin in cadherin turnover
Michael A Davis
Department of Cancer Biology, Vanderbilt University, Nashville, TN 37232 6840, USA
J Cell Biol 163:525-34. 2003
..The data reveal a core function of p120 in cadherin complexes, and strongly predict a dose-dependent loss of E-cadherin in tumors that partially or completely down-regulate p120...
- Discovery and development of the G-rich oligonucleotide AS1411 as a novel treatment for cancer
Paula J Bates
James Graham Brown Cancer Center, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
Exp Mol Pathol 86:151-64. 2009
..Thus, the serendipitous discovery of the G-rich oligonucleotides also led to the identification of nucleolin as a new molecular target for cancer therapy...
- Regulation of planar cell polarity by Smurf ubiquitin ligases
Center for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, Canada, M5G 1X5
Cell 137:295-307. 2009
..Our work thus uncovers ubiquitin ligases in a mechanistic link between noncanonical Wnt signaling and PCP/CE...
- Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolism
Jian Q Feng
Oral Biology, University of Missouri Kansas City, Kansas City, Missouri 64108, USA
Nat Genet 38:1310-5. 2006
..Our findings suggest a bone-renal axis that is central to guiding proper mineral metabolism...
- Expression of Yes-associated protein in common solid tumors
Angela A Steinhardt
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
Hum Pathol 39:1582-9. 2008
- Postreplicative recruitment of cohesin to double-strand breaks is required for DNA repair
Department of Cell and Molecular Biology, Karolinska Institute, Berzelius vag 35, 171 77 Stockholm, Sweden
Mol Cell 16:1003-15. 2004
..Evidence is also provided that DSB induction elicits establishment of sister chromatid cohesion in G2, implicating that damage-recruited cohesin facilitates DNA repair by tethering chromatids...
- Tumor suppressor LATS1 is a negative regulator of oncogene YAP
Department of Pathology and Molecular Medicine, Queen s University, Kingston, Ontario K7L 3N6, Canada
J Biol Chem 283:5496-509. 2008
..These research findings will have profound impacts on our understanding of the molecular mechanism of the LATS tumor suppressor and the emerging Hippo-LATS/Warts pathway...
- Disruption of IRS-2 causes type 2 diabetes in mice
D J Withers
Howard Hughes Medical Institute, Joslin Diabetes Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
Nature 391:900-4. 1998
..Our results indicate that dysfunction of IRS-2 may contribute to the pathophysiology of human type 2 diabetes...
- Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways
J D Axelrod
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 USA
Genes Dev 12:2610-22. 1998
..The PCP signal may selectively result in focal Fz activation and asymmetric relocalization of Dsh to the membrane, where Dsh effects cytoskeletal reorganization to orient prehair initiation...
- Analytical strategies for phosphoproteomics
Tine E Thingholm
KMEB, Department of Endocrinology, Odense University Hospital, Odense M, Denmark
Proteomics 9:1451-68. 2009
..Phosphorylation is a transient modification, and phosphoproteins are often very low abundant...
- Molecular architecture of SMC proteins and the yeast cohesin complex
Christian H Haering
Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
Mol Cell 9:773-88. 2002
..The two heads of the V-shaped dimer are connected by different ends of the cleavable Scc1 subunit. Cohesin therefore forms a large proteinaceous loop within which sister chromatids might be entrapped after DNA replication...
- Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways
Division of Oncology Research, Mayo Clinic, Rochester, Minnesota 55905, USA
J Cell Biol 153:613-20. 2001
..Third, 53BP1 is readily phosphorylated by ATM in vitro. Taken together, these results suggest that 53BP1 is an ATM substrate that is involved early in the DNA damage-signaling pathways in mammalian cells...
- Serine phosphorylation of insulin receptor substrate 1 by inhibitor kappa B kinase complex
Pennington Biomedical Research Center, Louisiana State University, Baton Rouge 70808, USA
J Biol Chem 277:48115-21. 2002
- Identification and characterization of nucleolin as a c-myc G-quadruplex-binding protein
College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA
J Biol Chem 284:23622-35. 2009
..Finally, we show that nucleolin binds to the c-myc promoter in HeLa cells, which indicates that this interaction occurs in vivo. In summary, nucleolin may induce c-myc G4 formation in vivo...
- Maturation of active zone assembly by Drosophila Bruchpilot
Institute for Biology Genetics, Free University Berlin, Berlin, Germany
J Cell Biol 186:129-45. 2009
..BRP seems responsible for effectively clustering Ca(2+) channels beneath the T-bar density late in a protracted AZ formation process, potentially through a direct molecular interaction with intracellular Ca(2+) channel domains...
- Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin
Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
Cell 123:49-63. 2005
..These findings demonstrate the importance of increased translation of p53 in DNA-damage responses and suggest critical roles for RPL26 and nucleolin in affecting p53 induction...
- Heterogeneous nuclear ribonucleoprotein K and nucleolin as transcriptional activators of the vascular endothelial growth factor promoter through interaction with secondary DNA structures
Diana J Uribe
Pharmacology and Toxicology Department, University of Arizona, Tucson, Arizona 85721, USA
Biochemistry 50:3796-806. 2011
..Taken together, the identification of transcription factors that can recognize and bind to atypical DNA structures within the pPu/pPy tract will provide new insight into mechanisms of transcriptional regulation of the VEGF gene...
- Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
Mol Biol Cell 6:1061-75. 1995
..The conformationally inaccessible C-terminal region included the F-actin binding site, suggesting that this activity is likewise regulated by masking...
- Mst1 and Mst2 maintain hepatocyte quiescence and suppress hepatocellular carcinoma development through inactivation of the Yap1 oncogene
Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
Cancer Cell 16:425-38. 2009
..Approximately 30% of human HCCs show low Yap1(Ser127) phosphorylation and a majority exhibit loss of cleaved, activated Mst1. Mst1/2 inhibition of Yap1 is an important pathway for tumor suppression in liver relevant to human HCC...
- Dynamic interactions between 14-3-3 proteins and phosphoproteins regulate diverse cellular processes
MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
Biochem J 381:329-42. 2004
- Mutations in the gene encoding the 3'-5' DNA exonuclease TREX1 cause Aicardi-Goutières syndrome at the AGS1 locus
Yanick J Crow
Leeds Institute of Molecular Medicine, University of Leeds, St James s University Hospital, Leeds LS9 7TF, UK
Nat Genet 38:917-20. 2006
..Our findings suggest an unanticipated role for TREX1 in processing or clearing anomalous DNA structures, failure of which results in the triggering of an abnormal innate immune response...
- Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2
Research Institute of Molecular Pathology, Vienna, Austria
PLoS Biol 3:e69. 2005
..The similarity of the phenotype obtained after expression of nonphosphorylatable SA2 in human cells to that seen after the depletion of Plk1 suggests that SA2 is the critical target of Plk1 in the cohesin dissociation pathway...
- Cellular levels of p120 catenin function as a set point for cadherin expression levels in microvascular endothelial cells
Department of Dermatology, School of Medicine, Emory University, Atlanta, GA 30322, USA
J Cell Biol 163:535-45. 2003
..These results demonstrate that cellular levels of p120ctn function as a set point mechanism that regulates cadherin expression levels, and that a major function of p120ctn is to control cadherin internalization and degradation...
- A WW domain-containing yes-associated protein (YAP) is a novel transcriptional co-activator
Department of Viral Oncology, Institute for Virus Research, Kyoto University, Shogo in, Sakyo ku, Kyoto 606 8507, Japan
EMBO J 18:2551-62. 1999
..These results indicate that the PY motif is a novel transcription activation domain that functions by recruiting YAP as a strong transcription activator to target genes...
- PhosPhAt: the Arabidopsis thaliana phosphorylation site database. An update
Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Golm, Germany
Nucleic Acids Res 38:D828-34. 2010
..Finally, we have implemented functional annotation of proteins using MAPMAN ontology. These new developments make the PhosPhAt resource a useful and powerful tool for the scientific community as a whole beyond the plant sciences...
- Perp is a p63-regulated gene essential for epithelial integrity
Rebecca A Ihrie
Division of Radiation and Cancer Biology, Stanford University School of Medicine, Stanford, California 94305, USA
Cell 120:843-56. 2005
..These findings demonstrate that Perp is a key effector in the p63 developmental program, playing an essential role in an adhesion subprogram central to epithelial integrity and homeostasis...
- Comparative analysis reveals conserved protein phosphorylation networks implicated in multiple diseases
Chris Soon Heng Tan
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5
Sci Signal 2:ra39. 2009
..proteins tightly regulated by phosphorylation as well as signal integration hubs, and both types of phosphoproteins were enriched in proteins encoded by disease-associated genes...
- Zonula occludens-1 and -2 are cytosolic scaffolds that regulate the assembly of cellular junctions
Alan S Fanning
The Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7545, USA
Ann N Y Acad Sci 1165:113-20. 2009
..Finally, we propose a model by which the functional activities of ZO proteins in the adherens junction and tight junction are differentiated by a novel regulatory motif known as the U6 or acidic motif...
- Disjunction of homologous chromosomes in meiosis I depends on proteolytic cleavage of the meiotic cohesin Rec8 by separin
S B Buonomo
Research Institute of Molecular Pathology, University of Vienna, Austria
Cell 103:387-98. 2000
..We show here that cleavage of Rec8 by separin at one of two different sites is necessary for the resolution of chiasmata and the disjunction of homologous chromosomes during meiosis...
- Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis
Howard Hughes Medical Institute and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
Nature 394:793-7. 1998
..We propose that epsin may participate, together with Eps15, in the molecular rearrangement of the clathrin coats that are required for coated-pit invagination and vesicle fission...
- RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion
Esther M Lafuente
Department of Medical Oncology, Dana Farber Cancer Institute, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Dev Cell 7:585-95. 2004
..RIAM knockdown displaced Rap1-GTP from the plasma membrane and abrogated Rap1-induced adhesion. Thus, RIAM links Rap1 to integrin activation and plays a role in regulating actin dynamics...
- Yes-associated protein 1 exhibits oncogenic property in gastric cancer and its nuclear accumulation associates with poor prognosis
Department of Anatomical and Cellular Pathology, Sir YK Pao Center for Cancer, State Key Laboratory in Oncology in South China, Institute of Digestive Disease and Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong
Clin Cancer Res 17:2130-9. 2011
..The role of YAP1 in tumorigenesis is unclear. We aimed to investigate the functional role of YAP1 in tumorigenesis of gastric cancer...
- Crowdsourcing network inference: the DREAM predictive signaling network challenge
Robert J Prill
IBM Computational Biology Center, Yorktown Heights, NY, 10598, USA
Sci Signal 4:mr7. 2011
..We conclude that crowdsourcing the construction of PSNs-that is, outsourcing the task to the interested community-may be an effective strategy for network inference...
- Focal adhesion kinase: in command and control of cell motility
Satyajit K Mitra
The Scripps Research Institute, Department of Immunology, IMM21 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nat Rev Mol Cell Biol 6:56-68. 2005
..Through multifaceted and diverse molecular connections, FAK can influence the cytoskeleton, structures of cell adhesion sites and membrane protrusions to regulate cell movement...
- Insulin signaling in health and disease
Morris F White
Howard Hughes Medical Institute, Joslin Diabetes Center, Harvard Medical School, 1 Joslin Place, Boston, MA 02215, USA
Science 302:1710-1. 2003
..Drugs that stimulate IRS2 (insulin receptor substrate protein 2) synthesis or signaling might be a good starting point. This knowledge will lead to rational strategies that prevent or cure diabetes...
- The Merlin/NF2 tumor suppressor functions through the YAP oncoprotein to regulate tissue homeostasis in mammals
Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Dev Cell 19:27-38. 2010
..Our studies link Merlin/NF2 to mammalian Hippo signaling and implicate YAP activation as a mediator of pathologies relevant to Neurofibromatosis 2...
- Cohesins form chromosomal cis-interactions at the developmentally regulated IFNG locus
Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
Nature 460:410-3. 2009
..This new aspect of cohesin function is probably important for normal development and disease...
- Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase
British Columbia Cancer Agency, Jack Bell Research Centre, 2660 Oak Street, Vancouver, British Columbia V6H 3Z6 Canada
Proc Natl Acad Sci U S A 95:11211-6. 1998
..ILK is thus a receptor-proximal effector for the Pi(3)K-dependent, extracellular matrix and growth factor mediated, activation of PKB/AKT, and inhibition of GSK-3...
- IFI16 acts as a nuclear pathogen sensor to induce the inflammasome in response to Kaposi Sarcoma-associated herpesvirus infection
H M Bligh Cancer Research Laboratories, Department of Microbiology and Immunology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, IL 60064, USA
Cell Host Microbe 9:363-75. 2011
..Caspase-1 activation by KSHV was reduced by IFI16 and ASC silencing. Our studies reveal IFI16 as a nuclear pathogen sensor and demonstrate that the inflammasome also functions in the nucleus...
- Phospho.ELM: a database of phosphorylation sites--update 2008
Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
Nucleic Acids Res 36:D240-4. 2008
..Phospho.ELM is available on line at: http://phospho.elm.eu.org...
- TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
Genes Dev 15:1229-41. 2001
..Because TEAD-dependent transcription was limited by YAP65, and YAP65 also binds Src/Yes protein tyrosine kinases, we propose that YAP65 regulates TEAD-dependent transcription in response to mitogenic signals...
- A novel role for p120 catenin in E-cadherin function
Renee C Ireton
Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
J Cell Biol 159:465-76. 2002
..These data reveal a cooperative interaction between p120 and E-cadherin and a novel role for p120 that is likely indispensable in normal cells...
- Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells
Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
J Cell Biol 153:263-72. 2001
- Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action
Biosignal Research Center, Kobe University, 657 8501, Kobe, Japan
Cell 110:177-89. 2002
..elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo...
- Mutations in VANGL1 associated with neural-tube defects
Department of Biochemistry, McGill University, Montreal, QC, Canada
N Engl J Med 356:1432-7. 2007
..In a protein-protein interaction assay V239I abolished interaction of VANGL1 protein with its binding partners, disheveled-1, -2, and -3. These findings implicate VANGL1 as a risk factor in human neural-tube defects...
- Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains
Department of Pharmacology, University of Wisconsin, Madison, Wisconsin 53706 1532, USA
J Biol Chem 273:31401-7. 1998
..We propose that Intersectin is a component of the endocytic machinery...
- Smad regulation in TGF-beta signal transduction
Ludwig Institute for Cancer Research, Box 595, SE 751 24 Uppsala, Sweden
J Cell Sci 114:4359-69. 2001
..Finally, the inhibitory Smads (I-Smads) block phosphorylation of R-Smads by the receptors and promote ubiquitination and degradation of receptor complexes, thus inhibiting signalling...