aequorin

Summary

Summary: A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350).

Top Publications

  1. ncbi Assay for intracellular calcium using a codon-optimized aequorin
    Wendy I Vernon
    AstraZeneca Pharmaceuticals, Cheshire, UK
    Biotechniques 33:730, 732, 734. 2002
  2. ncbi Modulation of histamine-induced Ca2+ release by protein kinase C. Effects on cytosolic and mitochondrial [Ca2+] peaks
    Mayte Montero
    Instituto de Biologia y Genetica Molecular, Departamento de Bioquimica y Biologia Molecular y Fisiologia, Facultad de Medicina, Universidad de Valladolid and Consejo Superior de Investigaciones Cientificas, Ramon y Cajal, 7, E 47005 Valladolid, Spain
    J Biol Chem 278:49972-9. 2003
  3. ncbi Development of a Ca(2+)-activated photoprotein, Photina, and its application to high-throughput screening
    Silvia Bovolenta
    Axxam SpA, San Raffaele Biomedical Science Park, Milan, Italy
    J Biomol Screen 12:694-704. 2007
  4. ncbi Subcellular calcium measurements in mammalian cells using jellyfish photoprotein aequorin-based probes
    Massimo Bonora
    1 Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, Interdisciplinary Center for the Study of Inflammation ICSI, Laboratory for Technologies of Advanced Therapies LTTA, University of Ferrara, Ferrara, Italy 2
    Nat Protoc 8:2105-18. 2013
  5. ncbi Biosensors for the detection of calcium and pH
    Paolo Pinton
    Department of Experimental and Diagnostic Medicine, Section of General Pathology, Interdisciplinary Center for the Study of Inflammation ICSI, University of Ferrara, I 44100 Ferrara, Italy
    Methods Cell Biol 80:297-325. 2007
  6. pmc Reorientation of seedlings in the earth's gravitational field induces cytosolic calcium transients
    Christoph Plieth
    Zentrum für Biochemie und Molekularbiologie, Christian Albrechts Universitat, Am Botanischen Garten 1 9 Room 517, D 24118 Kiel, Germany
    Plant Physiol 129:786-96. 2002
  7. pmc GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles
    Arancha Rodríguez-García
    Instituto de Biología y Genética Molecular IBGM, Universidad de Valladolid and Consejo Superior de Investigaciones Cientificas, 47003 Valladolid, Spain
    Proc Natl Acad Sci U S A 111:2584-9. 2014
  8. ncbi Isolation and characterization of a leucokinin-like peptide of Drosophila melanogaster
    S Terhzaz
    Laboratoire de Neuroendocrinologie des Insectes, Universite Bordeaux I, Bâtiment Recherche Biologie Animale, Avenue des Facultes, France
    J Exp Biol 202:3667-76. 1999
  9. pmc Aequorin-based luminescence imaging reveals stimulus- and tissue-specific Ca2+ dynamics in Arabidopsis plants
    Xiaohong Zhu
    Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
    Mol Plant 6:444-55. 2013
  10. ncbi The discovery of aequorin and green fluorescent protein
    O Shimomura
    The Photoprotein Laboratory, 324 Sippewissett Road, Falmouth, MA 02540, USA
    J Microsc 217:1-15. 2005

Detail Information

Publications195 found, 100 shown here

  1. ncbi Assay for intracellular calcium using a codon-optimized aequorin
    Wendy I Vernon
    AstraZeneca Pharmaceuticals, Cheshire, UK
    Biotechniques 33:730, 732, 734. 2002
  2. ncbi Modulation of histamine-induced Ca2+ release by protein kinase C. Effects on cytosolic and mitochondrial [Ca2+] peaks
    Mayte Montero
    Instituto de Biologia y Genetica Molecular, Departamento de Bioquimica y Biologia Molecular y Fisiologia, Facultad de Medicina, Universidad de Valladolid and Consejo Superior de Investigaciones Cientificas, Ramon y Cajal, 7, E 47005 Valladolid, Spain
    J Biol Chem 278:49972-9. 2003
    ..In addition, these mechanisms allow flexible and independent regulation of cytosolic and mitochondrial [Ca2+] during cell stimulation...
  3. ncbi Development of a Ca(2+)-activated photoprotein, Photina, and its application to high-throughput screening
    Silvia Bovolenta
    Axxam SpA, San Raffaele Biomedical Science Park, Milan, Italy
    J Biomol Screen 12:694-704. 2007
    ..produced a higher signal-to-noise ratio than conventional calcium dyes and a consistently stronger signal than aequorin when tested under equivalent conditions...
  4. ncbi Subcellular calcium measurements in mammalian cells using jellyfish photoprotein aequorin-based probes
    Massimo Bonora
    1 Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, Interdisciplinary Center for the Study of Inflammation ICSI, Laboratory for Technologies of Advanced Therapies LTTA, University of Ferrara, Ferrara, Italy 2
    Nat Protoc 8:2105-18. 2013
    The jellyfish Aequorea victoria produces a 22-kDa protein named aequorin that has had an important role in the study of calcium (Ca(2+)) signaling...
  5. ncbi Biosensors for the detection of calcium and pH
    Paolo Pinton
    Department of Experimental and Diagnostic Medicine, Section of General Pathology, Interdisciplinary Center for the Study of Inflammation ICSI, University of Ferrara, I 44100 Ferrara, Italy
    Methods Cell Biol 80:297-325. 2007
  6. pmc Reorientation of seedlings in the earth's gravitational field induces cytosolic calcium transients
    Christoph Plieth
    Zentrum für Biochemie und Molekularbiologie, Christian Albrechts Universitat, Am Botanischen Garten 1 9 Room 517, D 24118 Kiel, Germany
    Plant Physiol 129:786-96. 2002
    ..We now have made measurements of [Ca(2+)](c) in groups of young seedlings of Arabidopsis expressing aequorin in the cytoplasm and reconstituted in vivo with cp-coelenterazine, a synthetic high-affinity luminophore...
  7. pmc GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles
    Arancha Rodríguez-García
    Instituto de Biología y Genética Molecular IBGM, Universidad de Valladolid and Consejo Superior de Investigaciones Cientificas, 47003 Valladolid, Spain
    Proc Natl Acad Sci U S A 111:2584-9. 2014
    ..a novel family of fluorescent Ca(2+) sensors based on the fusion of two Aequorea victoria proteins, GFP and apo-aequorin (GAP)...
  8. ncbi Isolation and characterization of a leucokinin-like peptide of Drosophila melanogaster
    S Terhzaz
    Laboratoire de Neuroendocrinologie des Insectes, Universite Bordeaux I, Bâtiment Recherche Biologie Animale, Avenue des Facultes, France
    J Exp Biol 202:3667-76. 1999
    ..The gene resides at cytological location 70E3-70F4 of chromosome 3L. The localisation of this first Drosophila LK gene in a genetic model permits a genetic analysis of the locus...
  9. pmc Aequorin-based luminescence imaging reveals stimulus- and tissue-specific Ca2+ dynamics in Arabidopsis plants
    Xiaohong Zhu
    Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
    Mol Plant 6:444-55. 2013
    ..Here, an Aequorin-based luminescence recording system was developed for monitoring Ca(2+) in response to various stimuli in ..
  10. ncbi The discovery of aequorin and green fluorescent protein
    O Shimomura
    The Photoprotein Laboratory, 324 Sippewissett Road, Falmouth, MA 02540, USA
    J Microsc 217:1-15. 2005
  11. pmc Internal Ca(2+) release in yeast is triggered by hypertonic shock and mediated by a TRP channel homologue
    Valerie Denis
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 156:29-34. 2002
    ..These studies reveal a novel mechanism of internal Ca(2+) release and establish a new function for TRP channels...
  12. pmc Nod factors and chitooligomers elicit an increase in cytosolic calcium in aequorin-expressing soybean cells
    J Muller
    Friedrich Miescher Institut, P O Box 2543, CH 4002 Basel, Switzerland
    Plant Physiol 124:733-40. 2000
    ..Transgenic suspension-cultured soybean (Glycine max [L.] Merr.) cells expressing aequorin have been used to record cytosolic [Ca(2+)] changes upon treatment with purified NFs and chitin fragments...
  13. ncbi A dynamic pool of calcium in catecholamine storage vesicles. Exploration in living cells by a novel vesicle-targeted chromogranin A-aequorin chimeric photoprotein
    Nitish R Mahapatra
    Department of Medicine, University of California, San Diego, La Jolla 92093 0838, USA
    J Biol Chem 279:51107-21. 2004
    ..b>Aequorin, a jellyfish photoprotein with Ca(2+)-dependent luminescence, measures [Ca2+] in specific subcellular ..
  14. ncbi Stimulation by thimerosal of histamine-induced Ca(2+) release in intact HeLa cells seen with aequorin targeted to the endoplasmic reticulum
    M Montero
    , , Facultad de Medicina, Universidad de Valladolid, , E-47005 Valladolid, Spain
    Cell Calcium 30:181-90. 2001
    ..of thimerosal by monitoring the [Ca(2+)] inside the endoplasmic reticulum (ER) of intact HeLa cells with targeted aequorin. We show that thimerosal produced little effects on the ER-Ca(2+)-pump and only slightly increased the ER-Ca(2+)-..
  15. pmc Steady-state free Ca(2+) in the yeast endoplasmic reticulum reaches only 10 microM and is mainly controlled by the secretory pathway pump pmr1
    J Strayle
    , Pfaffenwaldring 55, D 70569 Stuttgart, Germany
    EMBO J 18:4733-43. 1999
    ..reticulum (ER) of yeast cells, using a specifically targeted version of the Ca(2+)-sensitive photoprotein aequorin. Ca(2+) uptake into the yeast ER displayed characteristics distinctly different from the mammalian ER...
  16. ncbi Functional characterisation of the Anopheles leucokinins and their cognate G-protein coupled receptor
    Jonathan C Radford
    Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, UK
    J Exp Biol 207:4573-86. 2004
    ..dose-dependent rise in intracellular calcium ([Ca2+]i) when applied to S2 cells co-expressing the receptor and an aequorin transgene, with a potency order of I>II>III...
  17. pmc The crystal structures of semi-synthetic aequorins
    Sachiko Toma
    Institute for Protein Research, Osaka University, 3 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Protein Sci 14:409-16. 2005
    The photoprotein aequorin emits light by an intramolecular reaction in the presence of a trace amount of Ca(2+)...
  18. ncbi Safety assessment of Apoaequorin, a protein preparation: subchronic toxicity study in rats
    Daniel L Moran
    Quincy Bioscience, 301 S Westfield Road, Suite 200, Madison, WI 53717, USA
    Food Chem Toxicol 57:1-10. 2013
    ..Based on the results of this study, the No Observed-Adverse-Effect Level (NOAEL) for Apoaequorin was determined as 666.7 mg/kg bw/day, the highest dose tested...
  19. ncbi Endothelin 1 enhances myofilament Ca2+ responsiveness in aequorin-loaded ferret myocardium
    J X Wang
    Charles A Dana Research Institute, Harvard Medical School, Boston, Mass
    Circ Res 69:582-9. 1991
    ..was investigated in ferret papillary muscles loaded with the Ca(2+)-regulated bioluminescent indicator aequorin. In concentrations of 3 x 10(-9) to 1 x 10(-7) M, endothelin produced dose-dependent increases in the amplitudes ..
  20. pmc Red fluorescent protein-aequorin fusions as improved bioluminescent Ca2+ reporters in single cells and mice
    Adil Bakayan
    Facultad de Medicina and Centro Regional de Investigaciones Biomédicas, University of Castilla La Mancha, Albacete, Spain
    PLoS ONE 6:e19520. 2011
    Bioluminescence recording of Ca(2+) signals with the photoprotein aequorin does not require radiative energy input and can be measured with a low background and good temporal resolution...
  21. ncbi Use of aequorin for G protein-coupled receptor hit identification and compound profiling
    Stephen J Brough
    Department of Screening and Compound Profiling, GlaxoSmithKline, Essex, UK
    Methods Mol Biol 552:181-98. 2009
    ..Investigations into the bioluminescence of jelly fish in the early 1960s isolated the photoprotein aequorin that required only the addition of calcium to generate a luminescent signal...
  22. ncbi Determination of affinity and activity of ligands at the human neuropeptide Y Y4 receptor by flow cytometry and aequorin luminescence
    Ralf Ziemek
    Institut fur Pharmazie, Universitat Regensburg, Regensburg, Germany
    J Recept Signal Transduct Res 27:217-33. 2007
    ..b>Aequorin luminescence was quantified in a microplate reader and by a CCD camera...
  23. ncbi Characterization of the 5-HT2b receptor in evaluation of aequorin detection of calcium mobilization for miniaturized GPCR high-throughput screening
    Mark A Gilchrist
    HTS CoE, Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    J Biomol Screen 13:486-93. 2008
    ..The data presented here demonstrate that the calcium-sensitive photoprotein aequorin (AequoScreen), when compared with FLUO-4 in the same cellular background, allows for miniaturization of ..
  24. ncbi Adaptation of aequorin functional assay to high throughput screening
    Emmanuel Le Poul
    Euroscreen s a, Brussels, Belgium
    J Biomol Screen 7:57-65. 2002
    AequoScreen, a cellular aequorin-based functional assay, has been optimized for luminescent high-throughput screening (HTS) of G protein-coupled receptor (GPCRs)...
  25. ncbi Analysis of C-terminally substituted tachykinin-like peptide agonists by means of aequorin-based luminescent assays for human and insect neurokinin receptors
    Herbert Torfs
    Laboratory for Developmental Physiology and Molecular Biology, Zoological Institute K U Leuven, Naamsestraat 59, B 3000, Leuven, Belgium
    Biochem Pharmacol 63:1675-82. 2002
    b>Aequorin-based assays for stable fly, Stomoxys calcitrans, (STKR) and human (neurokinin receptor 1 (NK1), neurokinin receptor 2 (NK2)) neurokinin-like receptors were employed to investigate the impact of a C-terminal amino acid exchange ..
  26. pmc The Ca2+ concentration of the endoplasmic reticulum is a key determinant of ceramide-induced apoptosis: significance for the molecular mechanism of Bcl-2 action
    P Pinton
    Department of Biomedical Sciences and CNR Center for the Study of Biomembranes, University of Padova, Via Colombo 3, I-35121 Padova, Italy
    EMBO J 20:2690-701. 2001
    ..It is therefore concluded that the Bcl-2-dependent reduction of [Ca2+]er is an important component of the anti-apoptotic program controlled by this oncogene...
  27. ncbi Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase
    Satoshi Inouye
    Yokohama Research Center, Chisso Co, 5 1 Okawa, Kanazawa Ku, Yokohama 236 8605, Japan
    FEBS Lett 580:1977-82. 2006
    Blue fluorescent protein from the calcium-binding photoprotein aequorin (BFP-aq) is a complex of Ca2+ -bound apoaequorin and coelenteramide, and shows luminescence activity like a luciferase, catalyzing the oxidation of coelenterazine ..
  28. ncbi Glowing jellyfish, luminescence and a molecule called coelenterazine
    K Jones
    School of Applied Chemistry, Kingston University, Penrhyn Road, Kingston upon Thames, UK KT1 2EE
    Trends Biotechnol 17:477-81. 1999
    ..The luminescent system of the jellyfish Aequorea victoria consists of the photoprotein aequorin, which contains the molecule coelenterazine as a prosthetic group and shows considerable potential in this area.
  29. ncbi NorpA and itpr mutants reveal roles for phospholipase C and inositol (1,4,5)- trisphosphate receptor in Drosophila melanogaster renal function
    Valerie P Pollock
    Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, UK
    J Exp Biol 206:901-11. 2003
    ..calcium levels in intact tubules of wild-type and itpr mutants using targeted expression of the calcium reporter, aequorin, show that mutations in itpr attenuated both CAP(2b)- and Drosokinin-stimulated calcium responses...
  30. ncbi Visualization of local Ca2+ dynamics with genetically encoded bioluminescent reporters
    Kelly L Rogers
    Unité d Embryologie Moléculaire, CNRS URA 2578, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Eur J Neurosci 21:597-610. 2005
    ..b>Aequorin is a genetically encoded Ca(2+)-sensitive bioluminescent protein, however, its low quantum yield prevents dynamic ..
  31. ncbi Targeted recombinant aequorins: tools for monitoring [Ca2+] in the various compartments of a living cell
    M Brini
    Department of Biochemistry, University of Padova, 35121 Padova, Italy
    Microsc Res Tech 46:380-9. 1999
    ..in living cells has been greatly enhanced by the utilisation of a recombinant Ca(2+)-sensitive photoprotein, aequorin. Aequorin is a Ca2+ sensitive photoprotein of a coelenterate that, in the past, was widely employed to measure ..
  32. ncbi Calcium release from the Golgi apparatus and the endoplasmic reticulum in HeLa cells stably expressing targeted aequorin to these compartments
    L Missiaen
    Afdeling Fysiologie, K U Leuven Campus Gasthuisberg O N, Herestraat 49, B 3000 Leuven, Belgium
    Cell Calcium 36:479-87. 2004
    ..release from both these compartments was studied in HeLa cells stably expressing the luminescent Ca2+ indicator aequorin specifically targeted to these compartments...
  33. ncbi The vacuolar Ca2+/H+ exchanger Vcx1p/Hum1p tightly controls cytosolic Ca2+ levels in S. cerevisiae
    A Miseta
    Department of Microbiology, The University of Alabama at Birmingham, 35294, USA
    FEBS Lett 451:132-6. 1999
    ..This finding is consistent with the postulated role of Vcx1p as a high capacity, low affinity Ca2+ transporter and suggests that Vcx1p may act to attenuate the propagation of Ca2+ signals in this organism...
  34. ncbi Plant calcium signaling and monitoring: pros and cons and recent experimental approaches
    C Plieth
    Zentrum für Biochemie und Molekularbiologie, Christian Albrechts Universitat, Am Botanischen Garten 1 9, 24118 Kiel, Federal Republic of Germany
    Protoplasma 218:1-23. 2001
    ..Properties and capabilities as well as advantages and disadvantages of these indicators when used in plant systems are discussed. Finally, general experimental strategies are mentioned which are able to answer open questions raised here...
  35. ncbi A calcium signal is involved in heterocyst differentiation in the cyanobacterium Anabaena sp. PCC7120
    I Torrecilla
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Madrid 28049, Spain
    Microbiology 150:3731-9. 2004
    ..PCC7120. This paper reports the use of a recombinant strain of this organism expressing the photoprotein aequorin to monitor the intracellular free-calcium concentration during the course of heterocyst differentiation...
  36. pmc All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin
    Lu Deng
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Protein Sci 14:663-75. 2005
    The crystal structures of calcium-loaded apo-aequorin and apo-obelin have been determined at resolutions 1.7A and 2.2 A, respectively...
  37. ncbi Modulation of calcium signalling by intracellular organelles seen with targeted aequorins
    M T Alonso
    Instituto de Biologia y Genetica Molecular, Universidad de Valladolid y Consejo Superior de Investigaciones Cientificas, Valladolid, Spain
    Acta Physiol (Oxf) 195:37-49. 2009
    ..Aequorins emitting at different wavelengths, prepared by fusion either with green or red fluorescent protein, permit simultaneous and independent monitorization of the Ca(2+) signals in different subcellular domains within the same cell...
  38. ncbi Intracellular calcium and ventricular fibrillation. Studies in the aequorin-loaded isovolumic ferret heart
    Y Kihara
    Charles A Dana Research Institute, Boston, Mass
    Circ Res 68:1378-89. 1991
    ..isovolumic left ventricular pressure were simultaneously recorded in isolated intact ferret hearts loaded with aequorin, a bioluminescent protein...
  39. ncbi Aequorin functional assay for characterization of G-protein-coupled receptors: implementation with cryopreserved transiently transfected cells
    Bruce Jones
    Cephalon, Inc, Worldwide Discovery Research, 145 Brandywine Parkway, West Chester, PA 19380, USA
    Anal Biochem 400:184-9. 2010
    ..been performed using cell-permeable fluorescent dyes, although the use of the recombinant photoprotein aequorin (AEQ) as a Ca(2+) sensor has gained popularity with recent advances in instrumentation...
  40. ncbi Aequorin-based functional assays for G-protein-coupled receptors, ion channels, and tyrosine kinase receptors
    Vincent J Dupriez
    Euroscreen s a, 47 Rue Adrienne Bolland, B 6041 Gosselies, Belgium
    Receptors Channels 8:319-30. 2002
    b>Aequorin is a photoprotein originating from jellyfish, whose luminescent activity is dependent on the concentration of calcium ions...
  41. pmc Calcium dependence of aequorin bioluminescence dissected by random mutagenesis
    Ludovic Tricoire
    Laboratoire de Neurobiologie et Diversité Cellulaire, Centre National de la Recherche Scientifique, Unité Mixte de Rechérche 7637, Ecole Superieure de Physique et de Chimie Industrielles, 10 rue Vauquelin, 75005 Paris, France
    Proc Natl Acad Sci U S A 103:9500-5. 2006
    b>Aequorin bioluminescence is emitted as a rapidly decaying flash upon calcium binding. Random mutagenesis and functional screening were used to isolate aequorin mutants showing slow decay rate of luminescence...
  42. pmc Measurement of proteases using chemiluminescence-resonance-energy-transfer chimaeras between green fluorescent protein and aequorin
    J P Waud
    Department of Medical Biochemistry, Cardiff and Vale NHS Trust, Llandough Hospital, Llandough, Penarth, Vale of Glamorgan CF64 2XX, UK
    Biochem J 357:687-97. 2001
    ..Chimaeras were engineered with aequorin as the chemiluminescent energy donor and green fluorescent protein (GFP) or enhanced GFP as the energy acceptors, ..
  43. ncbi Calcium imaging with chemiluminescence
    R Creton
    Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    Microsc Res Tech 46:390-7. 1999
    ..It is argued that aequorin-based calcium imaging is the method of choice for exploratory studies, since it is extremely sensitive, can ..
  44. ncbi Identifying nonselective hits from a homogeneous calcium assay screen
    Kelly J Cassutt
    Hamamatsu Corp, Bridgewater, NJ, USA
    J Biomol Screen 12:285-7. 2007
    ..In summary, the results suggest that AequoScreen technology, IP-ONE HTRF, and multiplex assays are unique, orthogonal technologies to identify nonspecific hits...
  45. ncbi Bacterial expression of in vivo-biotinylated aequorin for direct application to bioluminometric hybridization assays
    Monique Verhaegen
    Department of Chemistry and Biochemistry, University of Windsor, Ontario, Canada N9B 3P4
    Anal Biochem 306:314-22. 2002
    We have constructed a plasmid suitable for bacterial expression of in vivo-biotinylated photoprotein aequorin. The biotin tag facilitates the isolation of aequorin from crude cell extract and the direct complexation of aequorin with ..
  46. ncbi An immunoassay for Leu-enkephalin based on a C-terminal aequorin-peptide fusion
    S K Deo
    Department of Chemistry, University of Kentucky, Lexington, Kentucky 40506-0055, USA
    Anal Chem 73:1903-8. 2001
    Recently we demonstrated that the fusion of an octapeptide to the C-terminus of a cysteine-free mutant of aequorin showed no inhibitory effect on the luminescence activity of the photoprotein...
  47. ncbi Bioluminescence DNA hybridization assay for Plasmodium falciparum based on the photoprotein aequorin
    Leslie Doleman
    Department of Chemistry, University of Kentucky, Lexington, Kentucky 40506 0055, USA
    Anal Chem 79:4149-53. 2007
    ..assay for the detection of Plasmodium falciparum, the most deadly species of malaria, using the photoprotein aequorin as a bioluminescent label has been developed...
  48. ncbi Recombinant aequorin with a reactive cysteine residue for conjugation with maleimide-activated antibody
    Satoshi Inouye
    Yokohama Research Center, Chisso Corporation, Kanazawa Ku, Yokohama 236 8605, Japan
    Anal Biochem 378:105-7. 2008
    The mutated recombinant aequorin with a reactive cysteine residue (Cys-aequorin) was highly purified and then conjugated with a maleimide-activated antibody without significant loss of luminescence activity...
  49. ncbi One-step purification and refolding of recombinant photoprotein aequorin by immobilized metal-ion affinity chromatography
    Kyriaki Glynou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece
    Protein Expr Purif 27:384-90. 2003
    ..The yield was 0.7-1 mg apoaequorin from a 50 ml bacterial culture. The kinetics of light emission of purified aequorin upon addition of Ca(2+) was typical of the commercial aequorin...
  50. ncbi Direct measurement of free Ca(2+) shows different regulation of Ca(2+) between the periplasm and the cytosol of Escherichia coli
    H E Jones
    Department of Medical Biochemistry, University of Wales College of Medicine, CF14 4XN, Cardiff, UK
    Cell Calcium 32:183-92. 2002
    ..Periplasmic free Ca(2+) was measured by targeting the Ca(2+)-activated photoprotein aequorin to this compartment using the N-terminal OmpT signal sequence...
  51. ncbi Fusion of Aequorea victoria GFP and aequorin provides their Ca(2+)-induced interaction that results in red shift of GFP absorption and efficient bioluminescence energy transfer
    Andrey Yu Gorokhovatsky
    Branch of Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia
    Biochem Biophys Res Commun 320:703-11. 2004
    ..The bioluminescence emitted by Aequorea victoria jellyfish is greenish while its single bioluminescent photoprotein aequorin emits blue light...
  52. ncbi Time of day modulates low-temperature Ca signals in Arabidopsis
    Antony N Dodd
    Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
    Plant J 48:962-73. 2006
    ..We measured [Ca(2+)](cyt) non-invasively using aequorin, and targeted aequorin to the guard cell using a guard cell-specific GAL4-green fluorescent protein enhancer trap ..
  53. ncbi Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein
    Galina A Stepanyuk
    Photobiology Laboratory, Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
    FEBS Lett 579:1008-14. 2005
    The bioluminescence spectra from the Ca2+-regulated photoproteins aequorin (lambdamax=469 nm) and obelin (lambdamax=482 nm) differ because aequorin has an H-bond from its Tyr82 to the bound coelenteramide, not present in obelin at the ..
  54. pmc Dense core secretory vesicles revealed as a dynamic Ca(2+) store in neuroendocrine cells with a vesicle-associated membrane protein aequorin chimaera
    K J Mitchell
    Department of Biochemistry, University of Bristol, BS8 1TD Bristol, United Kingdom
    J Cell Biol 155:41-51. 2001
    ..By constructing a vesicle-associated membrane protein 2-synaptobrevin.aequorin chimera, we show that in clonal pancreatic islet beta-cells: (a) increases in [Ca(2+)](c) cause a prompt increase ..
  55. ncbi Slow changes in cytosolic free Ca2+ in Escherichia coli highlight two putative influx mechanisms in response to changes in extracellular calcium
    H E Jones
    Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, UK
    Cell Calcium 25:265-74. 1999
    Free intracellular Ca2+ ([Ca2+]i) in Escherichia coli was measured using the bioluminescent protein aequorin. Overall, the bacteria maintained a tight control on their free [Ca2+]i...
  56. ncbi Calcium measurement in living filamentous fungi expressing codon-optimized aequorin
    G Nelson
    Fungal Cell Biology Group, Institute of Cell and Molecular Biology, University of Edinburgh, Rutherford Building, Edinburgh EH9 3JH, UK
    Mol Microbiol 52:1437-50. 2004
    ..We have developed the recombinant aequorin method for this purpose...
  57. pmc Recombinant aequorin and green fluorescent protein as valuable tools in the study of cell signalling
    A Chiesa
    University of Ferrara, Department of Experimental and Diagnostic Medicine, Section of General Pathology, Via L. Borsari 46, 44100 Ferrara, Italy
    Biochem J 355:1-12. 2001
    Luminous proteins include primary light producers, such as aequorin, and secondary photoproteins that in some organisms red-shift light emission for better penetration in space...
  58. ncbi Activation of the oxidative burst in aequorin-transformed Nicotiana tabacum cells is mediated by protein kinase- and anion channel-dependent release of Ca2+ from internal stores
    S G Cessna
    Biochemistry and Molecular Biology Program, Department of Chemistry, Purdue University, 1393 Brown Bldg, W. Lafayette, IN 47904, USA
    Planta 214:126-34. 2001
    The source of Ca2+ involved in transducing an oxidative-burst defense signal was examined in aequorin-transformed tobacco (Nicotiana tabacum L.) cells using modulators of Ca2+ entry...
  59. ncbi The crystal structure of the photoprotein aequorin at 2.3 A resolution
    J F Head
    Department of Physiology, Boston University School of Medicine, Massachusetts 02118, USA
    Nature 405:372-6. 2000
    b>Aequorin is a calcium-sensitive photoprotein originally obtained from the jellyfish Aequorea aequorea...
  60. ncbi Blue fluorescent protein from the calcium-sensitive photoprotein aequorin is a heat resistant enzyme, catalyzing the oxidation of coelenterazine
    Satoshi Inouye
    Yokohama Research Center, Chisso Corporation, 5 1 Okawa, Kanazawa Ku, Yokohama 236 8605, Japan
    FEBS Lett 577:105-10. 2004
    Blue fluorescent protein from the calcium-sensitive photoprotein aequorin (BFP-aq) was prepared and determined to be a heat resistant enzyme, catalyzing the luminescent oxidation of coelenterazine (luciferin) with molecular oxygen as a ..
  61. ncbi Bioluminescence immunoassay for cortisol using recombinant aequorin as a label
    Mara Mirasoli
    Department of Chemistry, University of Kentucky, Lexington 40506 0055, USA
    Anal Biochem 306:204-11. 2002
    ..of a solid-phase competitive immunoassay for cortisol in saliva, in which a mutant of the photoprotein aequorin has been used as a label. We have chemically conjugated cortisol to aequorin at different molar ratios...
  62. ncbi Stable interactions between mitochondria and endoplasmic reticulum allow rapid accumulation of calcium in a subpopulation of mitochondria
    Luisa Filippin
    Department of Biomedical Sciences and CNR Institute of Neuroscience, University of Padua, Viale G Colombo 3, 35121 Padua, Italy
    J Biol Chem 278:39224-34. 2003
    ..Ca2+ sensors ("Cameleons," "Camgaroos," and "Pericams") and of the classical Ca(2+)-sensitive photoprotein aequorin, all targeted to the mitochondrial matrix...
  63. ncbi Red-shifted aequorin-based bioluminescent reporters for in vivo imaging of Ca2 signaling
    Thomas Curie
    Unité d Embryologie Moléculaire, CNRS URA 2578, Institut Pasteur, Paris, France
    Mol Imaging 6:30-42. 2007
    ..The genetically encoded bioluminescent Ca(2+) reporter green fluorescent protein-aequorin (GA) allows noninvasive detection of intracellular Ca(2+) signaling in freely moving mice...
  64. ncbi Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development
    Chris Y Cheung
    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, People s Republic of China
    Int J Dev Biol 50:561-9. 2006
    When aequorin is microinjected into cleavage-stage zebrafish embryos, it is largely used up by ~24 hours. Thus, it is currently not possible to image Ca(2+) signals from later stages of zebrafish development using this approach...
  65. ncbi Measuring [Ca2+] in the endoplasmic reticulum with aequorin
    J Alvarez
    Departamento de Bioquimica y Biologia Molecular y Fisiologia, Facultad de Medicina, Instituto de Biologia y Genetica Molecular, Universidad de Valladolid, Ramon y Cajal, 7, E 47005, Valladolid, Spain
    Cell Calcium 32:251-60. 2002
    The photoprotein aequorin was the first probe used to measure specifically the [Ca(2+)] inside the lumen of the endoplasmic reticulum ([Ca(2+)](ER)) of intact cells and it provides values for the steady-state [Ca(2+)](ER), around 500 ..
  66. ncbi Organellar calcium signalling mechanisms in Drosophila epithelial function
    Shireen A Davies
    University of Glasgow, Glasgow G11 6NU, UK
    J Exp Biol 212:387-400. 2009
    ..We also discuss recent findings on the roles of plasma membrane and intracellular calcium channels; and on organellar stores - including mitochondria, Golgi and peroxisomes - in Malpighian tubule function...
  67. ncbi Functional measurements of [Ca2+] in the endoplasmic reticulum using a herpes virus to deliver targeted aequorin
    M T Alonso
    Departamento de Bioquimica y Biologia Molecular y Fisiologia, Universidad de Valladolid y CSIC, Spain
    Cell Calcium 24:87-96. 1998
    ..We recently reported that recombinant aequorin targeted to the endoplasmic reticulum (ER) [Montero M., Brini M., Marsault R. et al...
  68. ncbi Aequorin-based homogeneous cortisol immunoassay for analysis of saliva samples
    Laura Rowe
    Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA
    Bioconjug Chem 18:1772-7. 2007
    ..we describe the development of a homogeneous assay for the hormone cortisol using the bioluminescent photoprotein aequorin as a reporter molecule...
  69. ncbi C-terminal and n-terminal fusions of aequorin with small peptides in immunoassay development
    S K Deo
    Department of Chemistry, University of Kentucky, Lexington, Kentucky 40506-0055, USA
    Bioconjug Chem 12:378-84. 2001
    b>Aequorin fusion proteins have been used extensively in intracellular Ca2+ measurements and in the development of binding assays...
  70. ncbi Aequorin luminescence-based assay for 5-hydroxytryptamine (serotonin) type 3 receptor characterization
    Jutta Walstab
    Institute of Pharmacology and Toxicology, University of Bonn, 53113 Bonn, Germany
    Anal Biochem 368:185-92. 2007
    ..the conditions for a sensitive assay suitable to measure 5-HT(3) receptor responses in cell suspension based on aequorin bioluminescence caused by Ca(2+) influx...
  71. pmc Aequorin variants with improved bioluminescence properties
    E Dikici
    Department of Chemistry, University of Kentucky, Lexington, KY 40506 0055, USA
    Protein Eng Des Sel 22:243-8. 2009
    The photoprotein aequorin has been widely used as a bioluminescent label in immunoassays, for the determination of calcium concentrations in vivo, and as a reporter in cellular imaging...
  72. pmc Mitochondrial priming modifies Ca2+ oscillations and insulin secretion in pancreatic islets
    E K Ainscow
    Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD
    Biochem J 353:175-80. 2001
    ..nutrient sensing in pancreatic islets and beta-cells by imaging adenovirally expressed Ca(2+) and ATP sensors (aequorin and firefly luciferase)...
  73. ncbi Monitoring mitochondrial [Ca(2+)] dynamics with rhod-2, ratiometric pericam and aequorin
    Rosalba I Fonteriz
    Instituto de Biología y Genética Molecular IBGM, Departamento de Bioquimica y Biologia Molecular y Fisiologia, Facultad de Medicina, Universidad de Valladolid and Consejo Superior de Investigaciones Cientificas CSIC, Ramon y Cajal, 7, E 47005 Valladolid, Spain
    Cell Calcium 48:61-9. 2010
    ..available to monitor [Ca(2+)](M) are fluorescent dyes such as rhod-2 and specifically targeted proteins such as aequorin and pericam...
  74. ncbi Ca2+ transport in mitochondria from yeast expressing recombinant aequorin
    Dennis W Jung
    Department of Molecular and Cellular Biochemistry, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Anal Biochem 324:258-68. 2004
    We have expressed aequorin in mitochondria of the yeast Saccharomyces cerevisiae and characterized the resulting strain with respect to mitochondrial Ca(2+) transport in vivo and in vitro...
  75. pmc Ca2+ homeostasis in the endoplasmic reticulum: coexistence of high and low [Ca2+] subcompartments in intact HeLa cells
    M Montero
    Department of Biochemistry and Molecular Biology and Physiology, Insituto de Biologia y Genetica Molecular, University of Valladolid and Consejo Superior de Investigaciones Cientificas, E 47005 Valladolid, Spain
    J Cell Biol 139:601-11. 1997
    Two recombinant aequorin isoforms with different Ca2+ affinities, specifically targeted to the endoplasmic reticulum (ER), were used in parallel to investigate free Ca2+ homeostasis in the lumen of this organelle...
  76. ncbi Control of secretion by mitochondria depends on the size of the local [Ca2+] after chromaffin cell stimulation
    M Montero
    , , , Facultad de Medicina, , E-47005 Valladolid, Spain
    Eur J Neurosci 13:2247-54. 2001
    ..By contrast, Ca2+ entry through P/Q-type channels fully activated mitochondrial Ca2+ uptake. Control of secretion by mitochondria therefore depends critically on the ability of the stimulus to create large local [Ca2+]c microdomains...
  77. pmc Bioluminescence imaging of mitochondrial Ca2+ dynamics in soma and neurites of individual adult mouse sympathetic neurons
    Lucía Núñez
    Instituto de Biologia y Genetica Molecular, Universidad de Valladolid and Consejo Superior de Investigaciones Cientificas, 47003 Valladolid, Spain
    J Physiol 580:385-95. 2007
    ..rapid, near millimolar [Ca(2+)](m) increases in a subpopulation of mitochondria containing about 40% of the aequorin. Caffeine evoked a similar [Ca(2+)](m) increase in a mitochondrial pool containing about 30% of the aequorin and ..
  78. ncbi Synthesis and characterization of potent and selective mu-opioid receptor antagonists, [Dmt(1), D-2-Nal(4)]endomorphin-1 (Antanal-1) and [Dmt(1), D-2-Nal(4)]endomorphin-2 (Antanal-2)
    Jakub Fichna
    Laboratory of Biomolecular Chemistry, Institute of Biomedicinal Chemistry, Medical University, Lodz, Poland
    J Med Chem 50:512-20. 2007
    ..Interestingly, another analogue of this series, [Dmt1, d-1-Nal4]endomorphin-1, turned out to be a moderately potent mixed mu-agonist/delta-antagonist...
  79. ncbi NMDA-evoked consumption and recovery of mitochondrially targeted aequorin suggests increased Ca2+ uptake by a subset of mitochondria in hippocampal neurons
    Kyle T Baron
    Department of Pharmacology, University of Minnesota Medical School, 6 120 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455 0217, USA
    Brain Res 993:124-32. 2003
    ..We used recombinant aequorin targeted to mitochondria to report changes in matrix Ca(2+) in rat hippocampal neurons in culture...
  80. ncbi A cytoplasmic Ca2+ functional assay for identifying and purifying endogenous cell signaling peptides in Arabidopsis seedlings: identification of AtRALF1 peptide
    Miyoshi Haruta
    UW Biotechnology Center, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 47:6311-21. 2008
    ..endogenous cell signaling peptides regulating cytoplasmic Ca(2+) signaling, Arabidopsis seedlings expressing aequorin were used for an in vivo luminescence assay for Ca(2+) changes...
  81. ncbi Imidazole-assisted catalysis of luminescence reaction in blue fluorescent protein from the photoprotein aequorin
    Satoshi Inouye
    Yokohama Research Center, Chisso Corporation, 5 1 Okawa, Kanazawa Ku, Yokohama 236 8605, Japan
    Biochem Biophys Res Commun 354:650-5. 2007
    Blue fluorescent protein from the calcium-binding photoprotein aequorin (BFP-aq) is a dissociable complex of Ca(2+)-bound apoaequorin and coelenteramide, and is identified as a luciferase that catalyzes the oxidation of coelenterazine by ..
  82. pmc Self-reporting Arabidopsis expressing pH and [Ca2+] indicators unveil ion dynamics in the cytoplasm and in the apoplast under abiotic stress
    Dongjie Gao
    Institut für Pflanzenernährung und Bodenkunde, Christian Albrechts Universitat, D 24098 Kiel, Germany
    Plant Physiol 134:898-908. 2004
    ..For measurements of calcium ([Ca(2+)]), luminescent aequorin variants were expressed in fusion with pHluorins...
  83. ncbi Analysis of the transient increase in cytosolic Ca2+ during the action potential of higher plants with high temporal resolution: requirement of Ca2+ transients for induction of jasmonic acid biosynthesis and PINII gene expression
    Joachim Fisahn
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Mühlenberg 15, D 14476 Golm, Germany
    Plant Cell Physiol 45:456-9. 2004
    ..Pretreatment of potato plants with Ruthenium Red inhibited induction of jasmonic acid biosynthesis and PINII gene expression that was generally triggered by heat-activated action potentials...
  84. ncbi Substitution of conserved glycine residue by alanine in natural and synthetic neuropeptide ligands causes partial agonism at the stomoxytachykinin receptor
    Jeroen Poels
    Laboratory for Developmental Physiology, Genomics and Proteomics, Zoological Institute KU Leuven, Leuven, Belgium
    J Neurochem 90:472-8. 2004
    ..Interestingly, one of these peptides, which contains an Ala-residue instead of the conserved Gly-residue, also proved to be a potent, partial agonist for STKR...
  85. ncbi Electron-multiplying charge-coupled detector-based bioluminescence recording of single-cell Ca2+
    Kelly L Rogers
    Plate forme d Imagerie Dynamique, Imagopole, Institut Pasteur, Paris, France
    J Biomed Opt 13:031211. 2008
    ..Recombinant apo-aequorin cloned from Aequorea victoria was the first Ca2+ sensitive protein genetically targeted to subcellular ..
  86. ncbi Functional characterization of opioid receptor ligands by aequorin luminescence-based calcium assay
    Jakub Fichna
    Laboratory of Biomolecular Chemistry, Medical University of Lodz, Mazowiecka 6 8, 92 215 Lodz, Poland
    J Pharmacol Exp Ther 317:1150-4. 2006
    A functional assay, based on aequorin-derived luminescence triggered by receptor-mediated changes in intracellular calcium levels, was used to examine relative potency and efficacy of the mu-opioid agonists endomorphin-1, endomorphin-2, ..
  87. ncbi Copper elicits an increase in cytosolic free calcium in cultured tobacco cells
    Hiroki Inoue
    Laboratory for Adaptation and Resistance, Environmental Plant Research Group, Plant Science Center, RIKEN, 1 7 22 Suehiro, Tsurumi, Yokohama, Kanagawa 230 0045, Japan
    Plant Physiol Biochem 43:1089-94. 2005
    ..These results demonstrated that copper causes an influx of Ca(2+) through plasma membrane Ca(2+) channels, and that plasma membrane thiol groups play an important role in activating the Ca(2+) channels...
  88. ncbi Bioluminescence imaging of nuclear calcium oscillations in intact pancreatic islets of Langerhans from the mouse
    Carlos Villalobos
    Instituto de Biología y Genética Molecular IBGM, Facultad de Medicina, Universidad de Valladolid and CSIC, Facultad de Medicina, Spain
    Cell Calcium 38:131-9. 2005
    ..Here we have monitored nuclear calcium oscillations by bioluminescence imaging of targeted aequorin in individual cells within intact islets of Langerhans as well as in the whole islet...
  89. ncbi Medium-chain fatty acids as ligands for orphan G protein-coupled receptor GPR84
    Jinghong Wang
    Amgen Inc, South San Francisco, California 94080, USA
    J Biol Chem 281:34457-64. 2006
    ..Our results indicate a role for GPR84 in directly linking fatty acid metabolism to immunological regulation...
  90. ncbi Comparison of antagonist activity of spantide family at human neurokinin receptors measured by aequorin luminescence-based functional calcium assay
    Anna Janecka
    Department of Medicinal Chemistry, Medical University of Lodz, Mazowiecka 6 8, 92 215 Lodz, Poland
    Regul Pept 131:23-8. 2005
    ..The functional assay based on aequorin-derived luminescence triggered by receptor-mediated changes in Ca2+ levels was used to compare the effect of ..
  91. pmc A diffusible signal from arbuscular mycorrhizal fungi elicits a transient cytosolic calcium elevation in host plant cells
    Lorella Navazio
    Dipartimento di Biologia, Universita di Padova, 35131 Padova, Italy
    Plant Physiol 144:673-81. 2007
    ..We used soybean (Glycine max) cell cultures stably expressing the bioluminescent Ca(2+) indicator aequorin to detect intracellular Ca(2+) changes in response to the culture medium of spores of Gigaspora margarita ..
  92. ncbi Ca2+ signaling in HEK-293 and skeletal muscle cells expressing recombinant ryanodine receptors harboring malignant hyperthermia and central core disease mutations
    Marisa Brini
    Department of Biochemistry and Center for the Study of Biomembranes of the National Research Council CNR, University of Padova, Viale G Colombo 3, 35121 Padova, Italy
    J Biol Chem 280:15380-9. 2005
    ..Analysis of intracellular Ca(2+) pools was performed using aequorin probes targeted to the lumen of the endo/sarcoplasmic reticulum (ER/SR), to the mitochondrial matrix, or to the ..
  93. ncbi The deposition of suberin lamellae determines the magnitude of cytosolic Ca2+ elevations in root endodermal cells subjected to cooling
    Catherine A Moore
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
    Plant J 30:457-65. 2002
    ..Perturbations in [Ca2+]cyt were studied in transgenic Arabidopsis thaliana, expressing aequorin fused to a modified yellow fluorescent protein solely in root endodermal cells, during slow cooling of plants ..
  94. ncbi [D-1-Nal4]endomorphin-2 is a potent micro-opioid receptor antagonist in the aequorin luminescence-based calcium assay
    Jakub Fichna
    Laboratory of Biomolecular Chemistry, Medical University of Lodz, Mazowiecka 6 8, 92 215 Lodz, Poland
    Life Sci 79:1094-9. 2006
    A functional assay, based on aequorin-derived luminescence triggered by receptor-mediated changes in Ca(2+) levels, was used to examine relative potency and efficacy of the micro-opioid receptor antagonists...
  95. pmc Mitochondrial and cytosolic calcium dynamics are differentially regulated in plants
    David C Logan
    School of Biology, Sir Harold Mitchell Building, University of St Andrews, St Andrews KY16 9TH, UK
    Plant Physiol 133:21-4. 2003
  96. ncbi Endoplasmic reticulum stress and alteration in calcium homeostasis are involved in cadmium-induced apoptosis
    Marta Biagioli
    Laboratory of Cellular and Development Biology, Department of Biology, University of Pisa, Italy
    Cell Calcium 43:184-95. 2008
    ..We used aequorin-based probes specifically directed to intracellular organelles to study Ca(2+) changes during cadmium injury...
  97. ncbi Measurement of stress-induced Ca(2+) pulses in single aequorin-transformed tobacco cells
    S G Cessna
    Program for Biochemistry and Molecular Biology, Purdue University, W. Lafayette, IN 47907, USA
    Cell Calcium 30:151-6. 2001
    ..Individual aequorin-transformed cells were isolated from a tobacco suspension culture and placed directly on a sensitive photo-..
  98. ncbi Expression of recombinant aequorin as an intracellular calcium reporter in the phytopathogenic fungus Phyllosticta ampelicida
    B D Shaw
    Department of Botany, University of Georgia, Athens, Georgia 30602, USA
    Fungal Genet Biol 34:207-15. 2001
    ..Transgenic strains of P. ampelicida expressing apo-aequorin, a calcium-sensitive luminescent protein, were developed to monitor cytoplasmic free Ca(2+) ([Ca(2+)]c)...
  99. pmc Analysis and effects of cytosolic free calcium increases in response to elicitors in Nicotiana plumbaginifolia cells
    David Lecourieux
    Unité Mixte de Recherche Institut National de la Recherche Agronomique Université de Bourgogne, Biochimie, Biologie Cellulaire et Ecologie des Interactions Plantes Microorganismes, 17 rue de Sully, BP 86510, 21065 Dijon Cedex, France
    Plant Cell 14:2627-41. 2002
    ..Competition assays with different elicitins demonstrate that [Ca(2+)](cyt) increase is mediated by cryptogein-receptor interaction...
  100. ncbi Cloning and functional characterization of the guinea pig vanilloid receptor 1
    Jonathan Savidge
    Novartis Institute for Medical Sciences, 5 Gower Place, WC1E 6BN, London, UK
    Neuropharmacology 43:450-6. 2002
    ..5, and provided complete blockade of heat responses at 1 micro M. Thus, capsazepine can significantly inhibit calcium influx due to heat and pH 5.5 at guinea pig VR1 and human VR1 but is inactive against these activators at rat VR1...
  101. ncbi Recombinant expression of the plasma membrane Na(+)/Ca(2+) exchanger affects local and global Ca(2+) homeostasis in Chinese hamster ovary cells
    Marisa Brini
    Department of Biochemistry and Center for the Study of Biomembranes of the National Research Council, University of Padova, Viale G Colombo 3, 35121 Padova, Italy
    J Biol Chem 277:38693-9. 2002
    ..transiently expressed in Chinese hamster ovary cells, which do not contain an endogenous exchanger, together with aequorin chimeras that are targeted to different intracellular compartments to investigate intracellular Ca(2+) ..

Research Grants32

  1. Beyond GFP and aequorin: Ocean-wide study of fluorescent and luminous proteins
    Mikhail Matz; Fiscal Year: 2009
    ..e., sharing no homology with the previously cloned ones) genetically encoded fluorescent and luminescent markers which may become a basis for the new generation of in vivo labeling and detection technologies. ..
  2. Fluorometric Imaging Plate Reader (FLIPRtetra)
    Louis M Luttrell; Fiscal Year: 2010
    ..an optional adapter for addition of cell suspensions and enhanced luminescence sensitivity with the optional aequorin luminescence camera...
  3. Monitoring Neural Activity in Freely Behaving Zebrafish Larvae with Bioluminescen
    Florian Engert; Fiscal Year: 2013
    ..constituted in vivo with its substrate coelenterazine (CLZN) to form the Ca2+-sensitive bioluminescent sensor GFP-Aequorin (GA). Flashes of luminescence will then report spontaneous and evoked Ca2+ signals in the targeted neurons...
  4. CARDIAC & VASCULAR TOXICITY OF COCAINE
    James Morgan; Fiscal Year: 1991
    ..performed with cardiac and vascular tissue from swine and ferrets that has been loaded with the calcium indicator aequorin to directly measure intracellular levels of free, ionized Ca++...
  5. PATHOPHYSIOLOGY OF CARDIOMYOPATHY
    JUDITH GWATHMEY; Fiscal Year: 1991
    ..that intracellular Ca++ handling is abnormal in cardiomyopathies of various etiologies; the Ca++ indicator aequorin will be used to determine how changes in intracellular Ca++ handling are related to the progressive ..
  6. BIOLUMINESCENT PROTEINS AS LABELS IN DIAGNOSTIC ASSAYS
    MILTON CORMIER; Fiscal Year: 1992
    ..new generation of highly sensitive and stable biochemical labels based on the recombinant bioluminescent proteins aequorin and Renilla luciferase, which have recently been cloned and overproduced in our laboratory...
  7. MECHANISMS BY WHICH CHLOROPHENOLS ACT AS CYTOTOXINS
    E Bittar; Fiscal Year: 1991
    ..single barnacle muscle fiber as a preparation, together with tow cell-free model systems, viz, the photoprotein aequorin and firefly (luciferase-luciferin) to test the general working hypothesis that certain chlorophenols, e.g...
  8. ABNORMALITIES OF PLATELET CALCIUM IN DISEASE
    J Ware; Fiscal Year: 1991
    ..Since aequorin and the fluorescent probes indo-1, quin2, and fura-2 appear to measure different aspects of Ca++ homeostasis, ..
  9. Modified Photoproteins as Labels and Molecular Switches in Bioanalysis
    Sylvia Daunert; Fiscal Year: 2013
    ..Bioluminescent photoproteins, such as aequorin, possess great potential to provide with a solution to this new challenge because they can be detected at low ..
  10. Macromolecular Crystallography Data Collection Instrumen
    Hwai Chen Guo; Fiscal Year: 2002
    ..i) deciphering the cellular functions of aldolase, j) enzymes in the meta-fission pathway, k) photoprotein aequorin, 1) multiple antibiotic resistance repressor MarR, m) nucleosome assembly chaperones, n) the apoptosome and its ..
  11. INTRACELLULAR SODIUM/CALCIUM DURING REPERFUSION STATES
    Thomas Guarnieri; Fiscal Year: 1991
    ..or during calcium deprivation-calcium reflow; 2) continuously measuring intracellular calcium via microinjected aequorin during hypoxia-reoxygenation or calcium deprivation-calcium reflow...
  12. LUMINESCENCE DETECTION REAGENTS FOR FOOD PATHOGENS
    David Smith; Fiscal Year: 1992
    ..This new technology exploits the remarkable sensitivity of the recombinant, bioluminescent protein, aequorin, and the high affinity interactions of streptavidin and biotin...
  13. Monitoring chemosensory responses of GLP-1 enteroendocrine cells
    Bedrich Mosinger; Fiscal Year: 2007
    ..to nutrients by enteroendocrine cells in a transgenic mouse engineered to express the calcium sensor GFP-aequorin. The nutrients to be tested include glucose, fats, peptones, amino acids and some bitter compounds...
  14. DIASTOLIC RELAXATION IN ISCHEMIA/HYPERTROPHY/FAILURE
    William Grossman; Fiscal Year: 1992
    ..myocytes and myocardial samples by means of patch clamping (to measure sarcolemmal electrical events), and aequorin light emission (to assess intracellular calcium transients and levels)...
  15. CONTROL OF CALCIUM IN AIRWAY SMOOTH MUSCLE CELLS
    Hiroshi Yamaguchi; Fiscal Year: 1992
    ..rapidly developing non- invasive methods, notably a family of fluorescent indicators, as well as the traditional aequorin technique...
  16. Bringing light to taste coding
    Bedrich Mosinger; Fiscal Year: 2007
    ..responses of defined subsets of taste cells to tastants using transgenic mice that selectively express GFP-aequorin under taste specific promoters...
  17. AEQUORIN AS A LABEL IN BIOLUMINESCENCE IMMUNOASSAYS
    Sylvia Daunert; Fiscal Year: 1999
    ..and ligands, as well as the sensitivity associated with bioluminescence detection. The photoprotein aequorin will be used as the bioluminescent label...
  18. CA 2+ ELECTRODE MEASUREMENTS IN LIMULUS PHOTORECEPTORS
    Jeffrey Owen; Fiscal Year: 1980
    ..Ca changes have previously been determined qualitatively by others with the photoprotein aequorin and more quantitatively with the metallochromic indicator dye, arsenazo III...
  19. NA/CA2 EXCHANGE AND CAI IN THE PROXIMAL TUBULE
    Jesus Dominguez; Fiscal Year: 1990
    ..We propose to extend this work, by measuring Ca/i, using aequorin and the fluxes of 45Ca and 22Na in rat proximal tubules...
  20. Living Cell Arrays for Real Time Functional Genomics
    Martin Yarmush; Fiscal Year: 2009
    ..expression: the use of intact cells for the simultaneous temporal expression profiling of multiple genes using aequorin-type fluorescent proteins (AFP) in a massively parallel, high throughput format...
  21. COMPUTER-ASSISTED ANALYSES OF EXCITATION-CONTRACTION
    Stuart Taylor; Fiscal Year: 1990
    ..ionic currents measured by on-cell patch clamping, intracellular Ca2+ transients monitored via the photoprotein aequorin, bond formation (cross-bridge attachment) between contractile filaments, force generation, striation spacing, and ..
  22. INTRACELLULAR CALCIUM CONTROL OF CAMP SYNTHESIS
    DERMOT COOPER; Fiscal Year: 2004
    ..The present application proposes to develop adenylyl cyclase/ aequorin and adenylyl cyclase/ cameleon chimeras as localized sensors of Ca2+ and to develop olfactory cyclic nucleotide ..
  23. Targeting the Mitochondrial Calcium Uniporter, Phase I
    Douglas Pfeiffer; Fiscal Year: 2005
    ..In one, cDNA libraries are expressed in a yeast cell line that has been engineered to express the photoprotein aequorin in mitochondria...
  24. VOLATILE ANESTHETICS AND CARDIAC FUNCTION
    PHILIPPE HOUSMANS; Fiscal Year: 2001
    ..Moreover, these results might provide a rational basis of knowledge on which to formulate pharmacological action to reverse or prevent myocardial depression in surgical patients. ..
  25. VOLATILE ANESTHETICS IN PRESSURE OVERLOAD HYPERTROPHY
    James Hannon; Fiscal Year: 2002
    ....
  26. Lactoferrin for Immunomodulation of Systemic Inflammatory Response Syndrome
    Jeffrey Actor; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  27. Regulation of Cortisol by Mycobacterial Glycolipid TDM
    Jeffrey Actor; Fiscal Year: 2005
    ..abstract_text> ..
  28. Regulatory Role of Growth Hormone Secretagogue Receptor
    Roy Smith; Fiscal Year: 2007
    ..We hypothesize that clinical use of ghrelin mimetics will translate to improved cognitive function in the elderly. [unreadable] [unreadable] [unreadable]..