annexin a5

Summary

Summary: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.

Top Publications

  1. ncbi Stimulation of suicidal erythrocyte death by benzethonium
    Elisabeth Lang
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 28:347-54. 2011
  2. ncbi Increased erythrocyte phosphatidylserine exposure in sickle cell disease: flow-cytometric measurement and clinical associations
    B L Wood
    Department of Laboratory Medicine, University of Washington, Seattle 98195 7110, USA
    Blood 88:1873-80. 1996
  3. ncbi The NFĸB pathway inhibitors Bay 11-7082 and parthenolide induce programmed cell death in anucleated Erythrocytes
    Mehrdad Ghashghaeinia
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 27:45-54. 2011
  4. ncbi Dicoumarol activates Ca2+-permeable cation channels triggering erythrocyte cell membrane scrambling
    Syed M Qadri
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 28:857-64. 2011
  5. ncbi Regulation of erythrocyte survival by AMP-activated protein kinase
    Michael Foller
    Department of Physiology, University of Tubingen, Tubingen, Germany
    FASEB J 23:1072-80. 2009
  6. ncbi Enhanced suicidal erythrocyte death in mice carrying a loss-of-function mutation of the adenomatous polyposis coli gene
    Syed M Qadri
    Department of Physiology, University of Tubingen, Tubingen, Germany
    J Cell Mol Med 16:1085-93. 2012
  7. ncbi Circulating CD31+/Annexin V+ microparticles correlate with cardiovascular outcomes
    Jan Malte Sinning
    Medizinische Klinik und Poliklinik II, Universitatsklinikum Bonn, Sigmund Freud Str 25, 53105, Bonn, Germany
    Eur Heart J 32:2034-41. 2011
  8. ncbi Inhibition of erythrocyte cation channels by erythropoietin
    Svetlana Myssina
    Department of Physiology, University of Tubingen, Germany
    J Am Soc Nephrol 14:2750-7. 2003
  9. ncbi Stimulation of suicidal erythrocyte death by α-lipoic acid
    Shefalee K Bhavsar
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 26:859-68. 2010
  10. ncbi Beauvericin induced erythrocyte cell membrane scrambling
    Syed M Qadri
    Department of Physiology, University of Tubingen, Gmelinstr 5, 72076 Tubingen, Germany
    Toxicology 283:24-31. 2011

Detail Information

Publications309 found, 100 shown here

  1. ncbi Stimulation of suicidal erythrocyte death by benzethonium
    Elisabeth Lang
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 28:347-54. 2011
    ..Benzethonium (5 μM) significantly enhanced lactic acid formation but not ceramide abundance. The present observations disclose a novel effect of benzethonium, i.e. triggering of suicidal death of erythrocytes...
  2. ncbi Increased erythrocyte phosphatidylserine exposure in sickle cell disease: flow-cytometric measurement and clinical associations
    B L Wood
    Department of Laboratory Medicine, University of Washington, Seattle 98195 7110, USA
    Blood 88:1873-80. 1996
    ....
  3. ncbi The NFĸB pathway inhibitors Bay 11-7082 and parthenolide induce programmed cell death in anucleated Erythrocytes
    Mehrdad Ghashghaeinia
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 27:45-54. 2011
    ..In conclusion, the pharmacological inhibitors of NFκB, Bay 11-7082 and parthenolide, interfere with the survival of erythrocytes involving mechanisms other than disruption of NFκB-dependent gene expression...
  4. ncbi Dicoumarol activates Ca2+-permeable cation channels triggering erythrocyte cell membrane scrambling
    Syed M Qadri
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 28:857-64. 2011
    ..In conclusion, dicoumarol stimulates suicidal death of erythrocytes by stimulating Ca(2+) entry and subsequent triggering of Ca(2+) dependent cell membrane scrambling...
  5. ncbi Regulation of erythrocyte survival by AMP-activated protein kinase
    Michael Foller
    Department of Physiology, University of Tubingen, Tubingen, Germany
    FASEB J 23:1072-80. 2009
    ..The observations disclose a critical role of AMPK in the survival of circulating erythrocytes...
  6. ncbi Enhanced suicidal erythrocyte death in mice carrying a loss-of-function mutation of the adenomatous polyposis coli gene
    Syed M Qadri
    Department of Physiology, University of Tubingen, Tubingen, Germany
    J Cell Mol Med 16:1085-93. 2012
    ..The observations point to accelerated eryptosis and subsequent clearance of apc(Min/+) erythrocytes, which contributes to or even accounts for the enhanced erythrocyte turnover, anaemia and splenomegaly in those mice...
  7. ncbi Circulating CD31+/Annexin V+ microparticles correlate with cardiovascular outcomes
    Jan Malte Sinning
    Medizinische Klinik und Poliklinik II, Universitatsklinikum Bonn, Sigmund Freud Str 25, 53105, Bonn, Germany
    Eur Heart J 32:2034-41. 2011
    ..We evaluated whether MPs are an independent marker for cardiovascular events in patients with stable coronary artery disease (CAD)...
  8. ncbi Inhibition of erythrocyte cation channels by erythropoietin
    Svetlana Myssina
    Department of Physiology, University of Tubingen, Germany
    J Am Soc Nephrol 14:2750-7. 2003
    ..The effect could prolong the erythrocyte lifespan and may contribute to the enhancement of the erythrocyte number during erythropoietin therapy in dialysis patients...
  9. ncbi Stimulation of suicidal erythrocyte death by α-lipoic acid
    Shefalee K Bhavsar
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 26:859-68. 2010
    ..In conclusion, α-lipoic acid triggers eryptosis but by the same token counteracts eryptosis during oxidative stress. α-lipoic acid sensitive eryptosis may lead to anemia and derangements of microcirculation...
  10. ncbi Beauvericin induced erythrocyte cell membrane scrambling
    Syed M Qadri
    Department of Physiology, University of Tubingen, Gmelinstr 5, 72076 Tubingen, Germany
    Toxicology 283:24-31. 2011
    ..The present observations disclose novel effects of beauvericin, i.e. stimulation of Ca²+ entry with subsequent cell membrane scrambling and inhibition of Ca²+ activated K+ channels with blunting of cell shrinkage...
  11. ncbi Effect of casein kinase 1α activator pyrvinium pamoate on erythrocyte ion channels
    Yuliya Kucherenko
    Department of Physiology, University of Tubingen, Gmelinstrasse 5, Tubingen, Germany
    Cell Physiol Biochem 30:407-17. 2012
    ....
  12. ncbi Protein kinase CK1α regulates erythrocyte survival
    Christine Zelenak
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 29:171-80. 2012
    ..In conclusion, CK1 isoform α participates in the regulation of erythrocyte programmed cell death by modulating cytosolic Ca(2+) activity...
  13. ncbi Stimulation of suicidal erythrocyte death by methylglyoxal
    Jan P Nicolay
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 18:223-32. 2006
    ..In conclusion, methylglyoxal impairs energy production and anti-oxidative defense, effects contributing to the enhanced PS exposure of circulating erythrocytes and eventually resulting in anemia and deranged microcirculation...
  14. ncbi Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation
    R J Berckmans
    Department of Clinical Chemistry, Leiden University Medical Center, The Netherlands
    Thromb Haemost 85:639-46. 2001
    ..We conclude that microparticles in blood from healthy individuals support thrombin generation via TF- and FVII-independent pathways, and which may have an anticoagulant function...
  15. ncbi Hemotrophic mycoplasmas induce programmed cell death in red blood cells
    Kathrin M Felder
    Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland
    Cell Physiol Biochem 27:557-64. 2011
    ..suis is able to induce eryptotic processes it is concluded that eryptosis is one anemia-inducing factor during M. suis infections and, therefore, plays a significant role in the pathogenesis of infectious anemia due to HM infection...
  16. ncbi Dynamic adhesion of eryptotic erythrocytes to endothelial cells via CXCL16/SR-PSOX
    Oliver Borst
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Am J Physiol Cell Physiol 302:C644-51. 2012
    ..The present observations demonstrate that eryptotic erythrocytes adhere to endothelial cells of the vascular wall in part by interaction of phosphatidylserine exposed at the erythrocyte surface with endothelial CXCL16...
  17. ncbi Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages
    Bent Brachvogel
    Department of Cell and Matrix Biology, MCRI, 3052 Parkville Victoria, Australia
    Development 132:2657-68. 2005
    The annexin A5 gene (Anxa5) was recently found to be expressed in the developing and adult vascular system as well as the skeletal system...
  18. ncbi Annexin A5 stimulates autophagy and inhibits endocytosis
    Ghita Ghislat
    Laboratorio de Biologia Celular, Centro de Investigacion Principe Felipe, Valencia, Spain
    J Cell Sci 125:92-107. 2012
    ..of purified lysosomal membranes from mouse fibroblasts, a Ca(2+)-dependent phospholipid-binding protein, annexin A5, was found to increase on lysosomal membranes under starvation...
  19. ncbi Circulating endothelial microparticles as a marker of cerebrovascular disease
    Keun Hwa Jung
    Department of Neurology, Clinical Research Institute, Seoul National University Hospital Seoul, South Korea
    Ann Neurol 66:191-9. 2009
    ..Detailed profiling of these blood endothelial markers may adumbrate the pathogenesis of stroke or enable determination of the risk for stroke. We investigated EMP profiles in patients at risk for cerebrovascular disease...
  20. pmc Phosphatidylserine directly and positively regulates fusion of myoblasts into myotubes
    Jaemin Jeong
    Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720 1762, USA
    Biochem Biophys Res Commun 414:9-13. 2011
    ..In contrast, myoblasts exposed to PC liposomes produce long myotubes with low numbers of myonuclei. Moreover, pharmacological masking of PS on the myoblast surface inhibits fusion of these cells into myotubes in a dose-dependent manner...
  21. ncbi Influence of paclitaxel on parasitemia and survival of Plasmodium berghei infected mice
    Saisudha Koka
    Department of Physiology, University of Tubingen, Germany
    Cell Physiol Biochem 23:191-8. 2009
    ..In conclusion, paclitaxel decreases parasitemia and enhances survival of P. berghei infected mice, an effect, which may be due to stimulation of eryptosis and/or a direct toxic effect on the parasite...
  22. ncbi The majority of circulating platelet-derived microparticles fail to bind annexin V, lack phospholipid-dependent procoagulant activity and demonstrate greater expression of glycoprotein Ib
    David Ewan Connor
    Department of Haematology and Haematological Stem Cell Transplantation, St Vincent s Hospital, Sydney, New South Wales, Australia
    Thromb Haemost 103:1044-52. 2010
    ..The significance of annexin V negative microparticles is unclear, however, it is possible that they possess other activities aside from procoagulant phospholipid activity...
  23. ncbi Localization of deoxyglucose and annexin A5 in experimental atheroma correlates with macrophage infiltration but not lipid deposition in the lesion
    Yan Zhao
    Department of Nuclear Medicine, Graduate School of Medicine, Hokkaido University, Kita ku, Sapporo, Japan
    Mol Imaging Biol 13:712-20. 2011
    ..We compared the uptake of 2-deoxy-2-fluoro-D-[(14)C]glucose ([(14)C]FDG) and [(99m)Tc]HYNIC-annexin V ([(99m)Tc]annexin A5) with the lesion histology in apolipoprotein E knockout (apoE(-/-)) mice.
  24. ncbi Lactadherin binding and phosphatidylserine expression on cell surface-comparison with annexin A5
    Swapan K Dasgupta
    Department of Pathology and Medicine Thrombosis Research, Baylor College of Medicine, Houston, Texas, USA
    Transl Res 148:19-25. 2006
    ..The anionic phospholipid-binding protein, annexin A5, has been used to detect the presence of phosphatidylserine on the outer leaflet of the cell membrane...
  25. ncbi Effect of anandamide in Plasmodium Berghei-infected mice
    Diwakar Bobbala
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 26:355-62. 2010
    ..In conclusion, anandamide stimulated eryptosis of infected erythrocytes thus counteracting parasitaemia and a lethal course of the disease...
  26. ncbi p38 MAPK activation and function following osmotic shock of erythrocytes
    Sergios Gatidis
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 28:1279-86. 2011
    ..In conclusion, p38 kinase is expressed in erythrocytes and participates in the machinery triggering eryptosis following hyperosmotic shock...
  27. ncbi Sphingomyelinase-induced adhesion of eryptotic erythrocytes to endothelial cells
    Majed Abed
    Physiologisches Institut, Eberhard Karls Universitat Tubingen, Gmelinstr 5, D 72076 Tubingen, Germany
    Am J Physiol Cell Physiol 303:C991-9. 2012
    ..Thus exposure to sphingomyelinase or C6-ceramide triggers eryptosis followed by phosphatidylserine- and CXCL16-sensitive adhesion of eryptotic erythrocytes to HUVEC...
  28. ncbi Janus kinase 3 is expressed in erythrocytes, phosphorylated upon energy depletion and involved in the regulation of suicidal erythrocyte death
    Shefalee K Bhavsar
    Department of Physiology, University of Tubingen, Gmelinstrasse 5, Tubingen, Germany
    Cell Physiol Biochem 27:547-56. 2011
    ..The observations disclose a completely novel role of Janus kinase 3, i.e. the triggering of cell membrane scrambling in energy depleted erythrocytes...
  29. ncbi Sunitinib-sensitive suicidal erythrocyte death
    Nazneen Shaik
    Department of Physiology, University of Tuebingen, Tuebingen, Germany
    Cell Physiol Biochem 30:512-22. 2012
    ..Sunitinib, however, did not significantly modify cytosolic ATP and ceramide abundance. The present observations reveal that sunitinib is able to trigger suicidal death in erythrocytes even in the absence of nuclei and mitochondria...
  30. ncbi Modulation of the immune system by dying cells and the phosphatidylserine-ligand annexin A5
    Udo S Gaipl
    Department of Internal Medicine 3, University Hospital Erlangen, Erlangen Germany
    Autoimmunity 40:254-9. 2007
    ..Therefore, the use of proteins interfering with the clearance process may open up new vistas to improve immunization strategies and may help to understand the mechanisms of autoimmune diseases...
  31. ncbi Inhibition of Ca(2+) entry and suicidal erythrocyte death by naringin
    Nazneen Shaik
    Department of Physiology, University of Tuebingen, Tuebingen, Germany
    Cell Physiol Biochem 30:678-86. 2012
    ..In conclusion, naringin blunts the increase of cytosolic Ca(2+) concentration, the shrinkage, the cell membrane scrambling and thus the suicidal death of erythrocytes following energy depletion or oxidative stress...
  32. pmc Imaging the molecular signatures of apoptosis and injury with radiolabeled annexin V
    Francis G Blankenberg
    Department of Radiology Division of Pediatric Radiology, Lucile Salter Packard Children s Hospital, Stanford, California, USA
    Proc Am Thorac Soc 6:469-76. 2009
    ..The latest results from Phase II imaging trials with NSCLC and non-Hodgkin's lymphoma will be also be detailed. Finally, the potential future application of this tracer for the imaging of other pulmonary pathologies will be outlined...
  33. ncbi p53-independent NOXA induction overcomes apoptotic resistance of malignant melanomas
    Jian Zhong Qin
    Department of Pathology, Loyola University of Chicago Medical Center, Chicago, IL, USA
    Mol Cancer Ther 3:895-902. 2004
    ....
  34. pmc Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair
    Anthony Bouter
    Molecular Imaging and NanoBioTechnology, IECB, UMR 5248 CBMN CNRS University Bordeaux1 ENITAB, Talence F 33402, France
    Nat Commun 2:270. 2011
    ....
  35. ncbi Type 1 and type 2 diabetic patients display different patterns of cellular microparticles
    Florence Sabatier
    INSERM EMI 0019, Laboratory of Immunology and Hematology, UFR de Pharmacie, Universite de la Mediterranee, Marseille, France
    Diabetes 51:2840-5. 2002
    ..In type 1 diabetic patients, TMP-procoagulant activity could be involved in vascular complications. Moreover, its correlation with HbA(1c) reinforces the importance of an optimal glycemic control in type 1 diabetes...
  36. ncbi In vivo detection of apoptosis
    Francis G Blankenberg
    Division of Pediatric Radiology, Department of Radiology, Lucile Salter Packard Children s Hospital, Stanford, California, USA
    J Nucl Med 49:81S-95S. 2008
    ..Finally, the use of MRI, MR spectroscopy, and ultrasound as possible alternative imaging modalities for the imaging of apoptosis is addressed...
  37. ncbi Suppression of TNF-alpha-induced apoptosis by NF-kappaB
    D J Van Antwerp
    Laboratory of Genetics, Salk Institute, La Jolla, CA 92037, USA Jolla Institute for Allergy and Immu
    Science 274:787-9. 1996
    ..These findings suggest that a negative feedback mechanism results from TNF-alpha signaling in which NF-kappaB activation suppresses the signals for cell death...
  38. ncbi Transient expression of phosphatidylserine at cell-cell contact areas is required for myotube formation
    S M van den Eijnde
    Department of Molecular Cell Biology, Cardiovascular Research Institute Maastricht, University of Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands
    J Cell Sci 114:3631-42. 2001
    ..Our findings indicate that different mechanisms regulate PS exposure during apoptosis and muscle cell differentiation, and that surface exposed PS plays a crucial role in the process of myotube formation...
  39. pmc 99mTc Hynic-rh-Annexin V scintigraphy for in vivo imaging of apoptosis in patients with head and neck cancer treated with chemoradiotherapy
    Frank J P Hoebers
    Department of Radiotherapy, The Netherlands Cancer Institute Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, Amsterdam, The Netherlands
    Eur J Nucl Med Mol Imaging 35:509-18. 2008
    ..The purpose of this study was to determine the value of (99m)Tc Hynic-rh-Annexin-V-Scintigraphy (TAVS), a non-invasive in vivo technique to demonstrate apoptosis in patients with head and neck squamous cell carcinoma...
  40. ncbi Extracellular annexin A5: functions of phosphatidylserine-binding and two-dimensional crystallization
    Hugo O van Genderen
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University Maastricht, Maastricht, The Netherlands
    Biochim Biophys Acta 1783:953-63. 2008
    ..b>Annexin A5 has the ability to bind in a calcium-dependent manner to phosphatidylserine and to form a membrane-bound two-..
  41. ncbi Silibinin strongly synergizes human prostate carcinoma DU145 cells to doxorubicin-induced growth Inhibition, G2-M arrest, and apoptosis
    Anil K Tyagi
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Clin Cancer Res 8:3512-9. 2002
    ..In this study, we assessed whether silibinin synergizes the therapeutic potential of the chemotherapeutic drug doxorubicin against prostate cancer, the effectiveness of which is limited because of high systemic toxicity...
  42. ncbi Capacitation induces cyclic adenosine 3',5'-monophosphate-dependent, but apoptosis-unrelated, exposure of aminophospholipids at the apical head plasma membrane of boar sperm cells
    B M Gadella
    Institute of Biomembranes, Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3564 CM Utrecht, The Netherlands
    Biol Reprod 67:340-50. 2002
    ..Bicarbonate-induced phospholipid scrambling thus appears to be an important and early physiological event in the capacitation process...
  43. pmc Annexin A5-functionalized bimodal nanoparticles for MRI and fluorescence imaging of atherosclerotic plaques
    Geralda A F van Tilborg
    Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
    Bioconjug Chem 21:1794-803. 2010
    ..In the present study, we developed and applied a small micellar fluorescent annexin A5-functionalized nanoparticle for noninvasive magnetic resonance imaging (MRI) of PS exposing cells in ..
  44. ncbi Comparison of 99mTc-annexin A5 with 18F-FDG for the detection of atherosclerosis in ApoE-/- mice
    Yan Zhao
    Department of Nuclear Medicine, Graduate School of Medicine, Hokkaido University, North 15, West 7, Kita ku, Sapporo 060 8638, Japan
    Eur J Nucl Med Mol Imaging 34:1747-55. 2007
    99m)Tc-annexin A5, a marker of ongoing apoptosis, and (18)F-FDG, a marker of the increased metabolism of inflammatory cells, are supposed to be useful in the detection of metabolically active atheroma...
  45. ncbi Enzyme prodrug therapy designed to target L-methioninase to the tumor vasculature
    Brent D Van Rite
    Bioengineering Center, University of Oklahoma, Norman, USA
    Cancer Lett 301:177-84. 2011
    ..Thus, this new approach appears promising...
  46. pmc Visualization of cell death in mice with focal cerebral ischemia using fluorescent annexin A5, propidium iodide, and TUNEL staining
    Peyman Bahmani
    Department of Experimental Neurology, Center for Stroke Research Berlin, Small Animal Imaging Center, Charite University Medicine Berlin, Chariteplatz 1, Berlin, Germany
    J Cereb Blood Flow Metab 31:1311-20. 2011
    ..b>Annexin A5 has been suggested as a marker for imaging cell death under various disease conditions including stroke...
  47. pmc Engineered annexin A5 variants have impaired cell entry for molecular imaging of apoptosis using pretargeting strategies
    Lisette Ungethüm
    Department of Biochemistry of the Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    J Biol Chem 286:1903-10. 2011
    Phosphatidylserine (PS) on apoptotic cells is a target for diagnosis and therapy using annexin A5 (anxA5)...
  48. ncbi Diannexin, an annexin A5 homodimer, binds phosphatidylserine with high affinity and is a potent inhibitor of platelet-mediated events during thrombus formation
    M L Rand
    Division of Haematology Oncology, The Hospital for Sick Children, Toronto, Canada
    J Thromb Haemost 10:1109-19. 2012
    Shielding of procoagulant phosphatidylserine (PS) with annexin A5 attenuates thrombosis, but annexin A5 (35.7 kDa) is rapidly cleared from the circulation. In contrast, Diannexin, a 73...
  49. ncbi Association of annexin A5 with Na+/Ca2+ exchanger and caveolin-3 in non-failing and failing human heart
    Emmanuel Camors
    INSERM, U 689, CRCIL, IFR 139, CNRS Hôpital Lariboisière, 41, boulevard de la Chapelle, 75475 Paris Cedex 10, France
    J Mol Cell Cardiol 40:47-55. 2006
    b>Annexin A5 is a Ca2+ dependent phosphatidylserine binding protein mainly located in the T-tubules and sarcolemma of cardiomyocytes...
  50. ncbi Induction of programmed erythrocyte death by gambogic acid
    Adrian Lupescu
    Department of Physiology, University of Tubingen, Gmelinstrasse 5, Tubingen, Germany
    Cell Physiol Biochem 30:428-38. 2012
    ..The present observations disclose a novel effect of gambogic acid, i.e. stimulation of suicidal death of human erythrocytes or eryptosis, paralleled by Ca(2+)-entry, ceramide formation, cell shrinkage and phosphatidylserine-exposure...
  51. ncbi Annexin A5 is involved in migration and invasion of oral carcinoma
    Liane Wehder
    Core Unit Chip Application, Institute of Human Genetics and Anthropology, Jena University Hospital, Jena, Germany
    Cell Cycle 8:1552-8. 2009
    b>Annexin A5 is a Ca(2+)-binding protein which is involved in membrane organization and dynamics...
  52. ncbi Deficiency of annexins A5 and A6 induces complex changes in the transcriptome of growth plate cartilage but does not inhibit the induction of mineralization
    Daniele Belluoccio
    Murdoch Childrens Research Institute and Department of Paediatrics, University of Melbourne, and Royal Children s Hospital, Parkville, Victoria, Australia
    J Bone Miner Res 25:141-53. 2010
    ..These results indicate that annexins A5 and A6 may not represent the essential annexins that promote mineralization in vivo...
  53. ncbi Circulating endothelial microparticles correlate inversely with endothelial function in patients with ischemic left ventricular dysfunction
    Daniel Bulut
    Herzzentrum der Kliniken der Ruhr Universität Bochum, Standort St Josef Hospital, Gudrunstrasse 56, 44791 Bochum, Germany
    J Card Fail 14:336-40. 2008
    ..The patients were divided in 2 groups, those with preserved or normal function (n = 17; EF 65 +/- 6%) and those with reduced left ventricular (LV) function (n = 18; EF 36 +/- 11%)...
  54. ncbi Annexin-V imaging for noninvasive detection of cardiac allograft rejection
    J Narula
    Hahnemann University Hospital, Philadelphia, Pennsylvania, USA
    Nat Med 7:1347-52. 2001
    ..This study reveals the clinical feasibility and safety of annexin-V imaging for noninvasive detection of transplant rejection by targeting cell membrane phospholipid alterations that are commonly associated with the process of apoptosis...
  55. ncbi Nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase: a role in high glucose-induced apoptosis in retinal Müller cells
    Linda L Kusner
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio, USA
    Invest Ophthalmol Vis Sci 45:1553-61. 2004
    ..In this study, the role of nuclear translocation of GAPDH in hyperglycemia-induced apoptosis in retinal Müller cells and the ability of R-(-)-deprenyl to inhibit the translocation of GAPDH and apoptosis were investigated...
  56. pmc Annexin A5-conjugated polymeric micelles for dual SPECT and optical detection of apoptosis
    Rui Zhang
    Department of Experimental Diagnostic Imaging, University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA
    J Nucl Med 52:958-64. 2011
    ..The purpose of this study was to develop and evaluate a multimodal nanoplatform for the detection of apoptosis...
  57. ncbi Involvement of phosphatidylserine externalization in the down-regulation of c-myb expression in differentiating C2C12 cells
    Petr Kaspar
    Institute of Molecular Genetics, AS CR v v i, Videnska 1083, Prague 4, CZ 14220, Czech Republic
    Differentiation 76:245-52. 2008
    ..We, therefore, suggest that cell surface-exposed PS, which plays a role in the process of myotube formation, is also involved in the down-regulation of c-myb expression...
  58. ncbi In vivo imaging of apoptosis
    Francis G Blankenberg
    Department of Radiology Division of Pediatric Radiology, Lucile Salter Packard Children s Hospital, Stanford, CA 94304, USA
    Cancer Biol Ther 7:1525-32. 2008
    ....
  59. ncbi Annexin A5 therapy attenuates vascular inflammation and remodeling and improves endothelial function in mice
    Mark M Ewing
    Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands
    Arterioscler Thromb Vasc Biol 31:95-101. 2011
    b>Annexin A5 (AnxA5) has antithrombotic, antiapoptotic, and antiinflammatory properties; we investigated its effectiveness against vascular inflammation, remodeling, and dysfunction in accelerated atherosclerosis.
  60. pmc Longitudinal PET imaging of doxorubicin-induced cell death with 18F-Annexin V
    Shuo Hu
    Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China
    Mol Imaging Biol 14:762-70. 2012
    ....
  61. ncbi A minimally invasive, translational biomarker of ketamine-induced neuronal death in rats: microPET Imaging using 18F-annexin V
    Xuan Zhang
    Division of Neurotoxicology, National Center for Toxicological Research, U S Food and Drug Administration, Jefferson, Arkansas 72079, USA
    Toxicol Sci 111:355-61. 2009
    ....
  62. ncbi Annexin A5 directly interacts with amyloidogenic proteins and reduces their toxicity
    Sahar Bedrood
    Department of Biochemistry and Molecular Biology, Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    Biochemistry 48:10568-76. 2009
    ..In this study, we show that annexin A5 plays a role in interacting with and reducing the toxicity of the amyloidogenic proteins, h-IAPP and alpha-..
  63. pmc Collagen/annexin V interactions regulate chondrocyte mineralization
    Hyon Jong Kim
    Musculoskeletal Research Laboratories, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 283:10310-7. 2008
    ..Because annexin V is up-regulated during pathological mineralization events of articular cartilage, it is possible that these interactions also regulate pathological mineralization...
  64. ncbi (99)mTc-(CO)(3) His-annexin A5 micro-SPECT demonstrates increased cell death by irinotecan during the vascular normalization window caused by bevacizumab
    Christel Vangestel
    Department of Gastroenterology, Ghent University Hospital, Ghent, Belgium
    J Nucl Med 52:1786-94. 2011
    ..The efficacy of antihuman VEGF antibody (bevacizumab) with or without irinotecan was evaluated in a colorectal cancer xenograft using (99m)Tc-(CO)(3) His-annexin A5.
  65. ncbi Polymorphisms in the annexin A5 gene promoter in Japanese women with recurrent pregnancy loss
    Hironori Miyamura
    Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, 1 98 Dengakugakubo, Kutsukake cho, Toyoake, Aichi 470 1192, Japan
    Mol Hum Reprod 17:447-52. 2011
    Recent findings have raised the possibility that polymorphisms within the annexin A5 gene (ANXA5) promoter contribute to the etiology of recurrent pregnancy loss (RPL)...
  66. ncbi Ca2+-activated transbilayer movement of plasma membrane phospholipids in Leishmania donovani during ionomycin or thapsigargin stimulation
    Adrien Weingärtner
    Humboldt Universitat zu Berlin, Faculty of Mathematics and Natural Science I, Institute of Biology, Berlin, Germany
    Mol Biochem Parasitol 179:59-68. 2011
    ..Our studies further show that lipid scrambling is not activated by rapid exposure of promastigotes to higher physiological temperature that increases intracellular Ca(2+) levels...
  67. pmc Leishmania promastigotes lack phosphatidylserine but bind annexin V upon permeabilization or miltefosine treatment
    Adrien Weingärtner
    Institute of Biology, Humboldt Universitat zu Berlin, Berlin, Germany
    PLoS ONE 7:e42070. 2012
    ..Instead, we identified several other phospholipid classes such phosphatidic acid, phosphatidylethanolamine; phosphatidylglycerol and phosphatidylinositol as candidate lipids enabling annexin V staining...
  68. ncbi Sequential expression of annexin A5 in the vasculature and skeletal elements during mouse development
    B Brachvogel
    Abteilung für Experimentelle Medizin I, Nikolaus Fiebiger Zentrum, Friedrich Alexander Universitat Erlangen Nurnberg, Glückstrasse 6, 91054, Erlangen, Germany
    Mech Dev 109:389-93. 2001
    b>Annexin A5 (annexin V, anchorin CII) represents the prototype member of the large annexin family, characterized by its ability to interact with phospholipids in a calcium-dependent manner and to form calcium-specific ion channels...
  69. pmc Diannexin decreases inflammatory cell infiltration into the islet graft, reduces β-cell apoptosis, and improves early graft function
    Elaine Y Cheng
    Department of Surgery, New York Presbyterian Weill Cornell Medical Center, New York, NY, USA
    Transplantation 90:709-16. 2010
    ..The recombinant homodimer of human annexin V, diannexin, has completed a Phase II Clinical Trial in Kidney Transplantation (NCT00615966)...
  70. pmc Annexin A5 is not essential for skeletal development
    Bent Brachvogel
    Universitat Erlangen Nurnberg, Nikolaus Fiebiger Zentrum, Experimentelle Medizin I, Erlangen, Germany
    Mol Cell Biol 23:2907-13. 2003
    ..The intensively studied annexin A5 has been identified by its effects on blood coagulation, and subsequently, its function as a calcium-specific ..
  71. ncbi MBP-annexin V radiolabeled directly with iodine-124 can be used to image apoptosis in vivo using PET
    Bronwen Dekker
    Cancer Research UK UMIST, Department of Radiochemical Targeting and Imaging, Paterson Institute for Cancer Research, M20 4BX Manchester, UK
    Nucl Med Biol 32:241-52. 2005
    ..In conclusion, we have shown that MBP-annexin V and the phosphatidylserine ELISA are useful tools for the development of methods for radiolabeling annexin V for PET imaging...
  72. ncbi Leptin attenuates hypoxia/reoxygenation-induced activation of the intrinsic pathway of apoptosis in rat H9c2 cells
    Eyun Jung Shin
    Department of Biology, York University, Toronto, Ontario, Canada
    J Cell Physiol 221:490-7. 2009
    ..In conclusion, we establish mechanisms via which leptin exerts anti-apoptotic effects that may be of significance in understanding the development of heart failure in obesity...
  73. ncbi Crystal and molecular structure of human annexin V after refinement. Implications for structure, membrane binding and ion channel formation of the annexin family of proteins
    R Huber
    Max Planck Institut fur Biochemie, Martinsried, Germany
    J Mol Biol 223:683-704. 1992
    ..We suggest that annexins bind with their convex face to membranes, causing local disorder and permeability of the phospholipid bilayers. Annexins are Janus-faced proteins that face phospholipid and water and mediate calcium transport...
  74. ncbi Stereospecific interactions are necessary for Alzheimer disease amyloid-β toxicity
    Giuseppe D Ciccotosto
    Department of Pathology, The University of Melbourne, VIC 3010, Australia
    Neurobiol Aging 32:235-48. 2011
    ..This suggests that Aβ mediated toxicity in Alzheimer disease is dependent upon Aβ binding to phosphatidylserine on neuronal cells...
  75. ncbi Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells
    B Schutte
    Department of Molecular Cell Biology and Genetics, University of Maastricht, The Netherlands
    J Neurosci Methods 86:63-9. 1998
    ..In the present paper, we show that the annexin V-binding assay is also applicable to differentiated neuronal cells with fragile neurite outgrowths...
  76. ncbi PET and SPECT imaging of apoptosis in vulnerable atherosclerotic plaques with radiolabeled Annexin A5
    E M Laufer
    Department of Cardiology, Maastricht University Medical Center, Maastricht, The Netherlands
    Q J Nucl Med Mol Imaging 53:26-34. 2009
    ..Further development of apoptosis imaging technologies may aid us in the years to come in the quest to identify patients with critical cardiovascular risks, to treat myocardial infarction in its imminent, instead of its evident phase...
  77. ncbi Annexin A5 uptake in ischemic myocardium: demonstration of reversible phosphatidylserine externalization and feasibility of radionuclide imaging
    Heidi Kenis
    Cardiovascular Research Institute Maastricht, Maastricht, The Netherlands
    J Nucl Med 51:259-67. 2010
    ....
  78. ncbi Site-specific 68Ga-labeled Annexin A5 as a PET imaging agent for apoptosis
    Matthias Bauwens
    Radiopharmacy, KULeuven, Belgium
    Nucl Med Biol 38:381-92. 2011
    Two variants of Annexin A5 (Cys2-AnxA5 and Cys165-AnxA5) were labelled with Gallium-68 in order to evaluate their biological properties.
  79. ncbi Annexin A5 inhibits engulfment through internalization of PS-expressing cell membrane patches
    Heidi Kenis
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands
    Exp Cell Res 312:719-26. 2006
    ..Recently, we demonstrated that annexin A5 mediates the internalization of PS-expressing membrane patches and down regulates surface expression of tissue ..
  80. ncbi Phosphatidylserine is a marker of tumor vasculature and a potential target for cancer imaging and therapy
    Sophia Ran
    Simmons Comprehensive Cancer Center, The University of Texas Southwestern Medical Center, Dallas, TX 75390 8594, USA
    Int J Radiat Oncol Biol Phys 54:1479-84. 2002
    ..2) To determine whether PS exposure can be induced on viable endothelial cells in tissue culture by conditions present in the tumor microenvironment...
  81. ncbi Apoptosis imaging: beyond annexin V
    Gang Niu
    Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Nucl Med 51:1659-62. 2010
    ..It is highly possible that quantitative imaging of apoptosis will greatly improve clinical decision making in apoptosis-related diseases...
  82. ncbi Linking proteins with anionic nanoparticles via protamine: ultrasmall protein-coupled probes for magnetic resonance imaging of apoptosis
    Eyk Schellenberger
    Department of Radiology, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Small 4:225-30. 2008
    ..b>Annexin A5 (anxA5)-VSOP utilizing thiol chemistry was generated to couple biologically active anxA5 to VSOPs for in vivo ..
  83. ncbi Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa
    Uwe Paasch
    Department of Dermatology Andrology Unit, University of Leipzig, D 04103 Leipzig, Germany
    Biol Reprod 71:1828-37. 2004
    ..These results demonstrate that selection of annexin V-negative mature spermatozoa might be of clinical relevance for fertility preservation, as this sperm fraction shows no activated apoptosis during the cryopreservation process...
  84. ncbi Annexin V, annexin VI, S100A1 and S100B in developing and adult avian skeletal muscles
    C Arcuri
    Department of Experimental Medicine and Biochemical Sciences, Section of Anatomy, University of Perugia, Via del Giochetto, C P 81 Succ 3, 06122 Perugia, Italy
    Neuroscience 109:371-88. 2002
    ....
  85. ncbi In vitro measurement of cell death with the annexin A5 affinity assay
    Hugo van Genderen
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, PO Box 616, 6200 MD, Maastricht, The Netherlands
    Nat Protoc 1:363-7. 2006
    ..of phosphatidylserine at the cell surface can be measured in vitro with the phosphatidylserine-binding protein annexin A5 conjugated to fluorochromes...
  86. ncbi Evaluation of 18F-annexin V as a PET imaging agent in an animal model of apoptosis
    Kevin J Yagle
    Department of Radiology, Molecular Imaging Laboratory, University of Washington, Seattle, Washington 98195, USA
    J Nucl Med 46:658-66. 2005
    ..Because one of the earliest measurable events in apoptosis is the eversion of phosphatidylserine from the inner membrane leaflet to the outer cell surface, annexin V has proven useful for detecting the earliest stages of apoptosis...
  87. ncbi Rapid, noninflammatory and PS-dependent phagocytic clearance of necrotic cells
    U A Hirt
    Faculty of Biology, University of Konstanz, X911, D 78457 Konstanz, Germany
    Cell Death Differ 10:1156-64. 2003
    ..We demonstrate that this can occur independently of calcium signals, and that surface phosphatidylserine is essential for the uptake of necrotic cells by both human macrophages and murine microglia...
  88. ncbi Rosuvastatin increases extracellular adenosine formation in humans in vivo: a new perspective on cardiovascular protection
    Patrick Meijer
    Radboud University Nijmegen Medical Centre, Department of Pharmacology and Toxicology, Nijmegen, The Netherlands
    Arterioscler Thromb Vasc Biol 29:963-8. 2009
    ..In addition, we studied the effect of statin therapy in a forearm model of ischemia-reperfusion injury...
  89. ncbi Microparticles from ischemic muscle promotes postnatal vasculogenesis
    Aurelie S Leroyer
    Paris Cardiovascular Research Center, INSERM U, Hopital Europeen Georges Pompidou, Universite Paris Descartes, France
    Circulation 119:2808-17. 2009
    ..We hypothesized that microparticles (MPs) released after ischemia are endogenous signals leading to postischemic vasculogenesis...
  90. ncbi Flow cytometric measurement of microparticles: pitfalls and protocol modifications
    Mona D Shah
    Baylor College opf Medicine, Texas Children s Cancer Center and Hematology Service, Texas Children s Hospital, Houston, Texas 77030, USA
    Platelets 19:365-72. 2008
    ..These results illustrate the need to standardize assay protocols in order to obtain consistent measurements. Our studies further optimize sample preparation for microparticle detection...
  91. ncbi Differences in the biophysical properties of membrane and cytoplasm of apoptotic cells revealed using dielectrophoresis
    Fatima H Labeed
    Centre for Biomedical Engineering, School of Engineering, University of Surrey, Guildford, Surrey GU2 7XH, UK
    Biochim Biophys Acta 1760:922-9. 2006
    ..The use of dielectrophoresis for monitoring apoptosis offers a number of benefits as it is both rapid and non-invasive. It can also be used in parallel with other assays in high-throughput screening applications...
  92. ncbi Annexin A5: an imaging biomarker of cardiovascular risk
    Edward M Laufer
    Dept of Cardiology, University of Maastricht, Maastricht, The Netherlands
    Basic Res Cardiol 103:95-104. 2008
    ..b>Annexin A5, a 35 kD plasma protein, has strong affinity for PS in the nano-molar range...
  93. ncbi Lactadherin blocks thrombosis and hemostasis in vivo: correlation with platelet phosphatidylserine exposure
    J Shi
    Department of Medicine, VA Boston Healthcare System, Brigham, Women s Hospital and Harvard Medical School, Boston, MA 02132, USA
    J Thromb Haemost 6:1167-74. 2008
    ..We hypothesized that platelet PS exposure would be identified on adherent platelets at the site of vascular injury and that blockade of PS would impede hemostasis and thrombosis...
  94. pmc Characterization of the growth of 2D protein crystals on a lipid monolayer by ellipsometry and rigidity measurements coupled to electron microscopy
    C Venien-Bryan
    Institut de Biologie Structurale Jean Pierre Ebel CEA CNRS, Grenoble, France
    Biophys J 74:2649-57. 1998
    ....
  95. ncbi Nanosecond pulsed electric fields (nsPEF) induce direct electric field effects and biological effects on human colon carcinoma cells
    Emily H Hall
    Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital of the King s Daughters, Norfolk, Virginia 23510, USA
    DNA Cell Biol 24:283-91. 2005
    ..NsPEF applications provide a means to explore intracellular structures and functions that can reveal mechanisms in health and disease...
  96. pmc Age-related loss of phospholipid asymmetry in APP(NLh)/APP(NLh) x PS-1(P264L)/PS-1(P264L) human double mutant knock-in mice: relevance to Alzheimer disease
    Miranda L Bader Lange
    Department of Chemistry, University of Kentucky, Lexington, KY 40506 0055, USA
    Neurobiol Dis 38:104-15. 2010
    ..These results are discussed with relevance to loss of lipid asymmetry and consequent neurotoxicity in brain of subjects with Alzheimer disease...
  97. pmc Molecular MRI detects low levels of cardiomyocyte apoptosis in a transgenic model of chronic heart failure
    David E Sosnovik
    Center for Molecular Imaging Research, The Cardiology Division, and Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston, Masss, USA
    Circ Cardiovasc Imaging 2:468-75. 2009
    ..The myocardium in heart failure, however, is characterized by very low levels of CM apoptosis and normal vascular permeability, factors thought to preclude the use of molecular MRI...
  98. ncbi Annexin 5 messenger ribonucleic acid expression in pituitary gonadotropes is induced by gonadotropin-releasing hormone (GnRH) and modulates GnRH stimulation of gonadotropin release
    Mitsumori Kawaminami
    Department of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Japan
    Neuroendocrinology 75:2-11. 2002
    ..It is thus concluded that annexin 5 is synthesized in the gonadotropes under the effect of GnRH, and it is suggested that annexin 5 synthesis mediates at least partly GnRH receptor signaling to stimulate gonadotropin secretion...
  99. ncbi The role of annexin A5 in the modulation of the immune response against dying and dead cells
    Luis E Munoz
    Institute for Clinical Immunology and Exp Medicine I, Friedrich Alexander University of Erlangen Nuremberg, Glückstrasse 4a and 6, 91054 Erlangen, Germany
    Curr Med Chem 14:271-7. 2007
    Annexins are characterized by the ability to bind phospholipids of membranes in the presence of Ca2+. Annexin A5 represents a typical member of this protein family and is a natural occurring highly specific ligand for phosphatidylserine (..
  100. ncbi Virus-specific CD8 T cells in peripheral tissues are more resistant to apoptosis than those in lymphoid organs
    Xiaoting Z Wang
    Department of Pathology, University of Massachusetts Medical School, Worcester 01655, USA
    Immunity 18:631-42. 2003
    ..This may contribute to the survival of nondividing peripheral memory T cells, enabling them to efficiently function without being driven into apoptosis...
  101. ncbi Isolated Anxa5+/Sca-1+ perivascular cells from mouse meningeal vasculature retain their perivascular phenotype in vitro and in vivo
    Bent Brachvogel
    Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany
    Exp Cell Res 313:2730-43. 2007
    ..Using the specifically expressed annexin A5-LacZ fusion gene (Anxa5-LacZ), it became possible to isolate perivascular cells (PVC) from mouse tissues...

Research Grants62

  1. NOVEL BIOMARKERS FOR MECHANISTIC DIAGNOSIS OF THE ANTIPHOSPHOLIPID SYNDROME
    JACOB RAND; Fiscal Year: 2010
    ..of a mechanism - the antibody-mediated disruption of the potent vascular and placental anticoagulant protein, annexin A5 - has been defined, for which we developed a robust functional assay, the annexin A5 resistance (A5R) assay, ..
  2. Stroke and aPL: Community-Based Clinicopathological Study
    STEVEN RICHARD LEVINE; Fiscal Year: 2012
    ..preliminary data on potentially important mechanisms by which aPL may play a role, specifically through altered annexin A5 resistance, anti-annexin antibodies, and aPL binding to domain I (the thrombogenic domain) of [unreadable]2-..
  3. scVEGF Targeted Radiotherapy of Mammary and Colonic Cancer
    FRANCIS GERARD BLANKENBERG; Fiscal Year: 2010
    ..Successful completion of our proposal will give the needed preclinical data for pursuing scVEGF targeted radiotherapy in other models and ultimately clinical applications. ..
  4. IMAGING APOPTOSIS IN VIVO WITH TECHNETIUM 99M ANNEXIN
    Francis Blankenberg; Fiscal Year: 2001
    ..abstract_text> ..
  5. IMAGING APOPTOSIS IN VIVO WITH TECHNETIUM 99M ANNEXIN
    Francis Blankenberg; Fiscal Year: 2007
    ..Completion of this proposal will aid in the design and planning of clinical trials that will study the use of serial annexin V imaging as a non-invasive marker of cellular injury and therapeutic efficacy. ..
  6. Reduction of Annexin A5 in Antipholipid Pregnancy Loss
    JACOB RAND; Fiscal Year: 2010
    ..The placental anticoagulant protein, annexin A5 (AnxAS) is a potent phospholipid (PL)-binding protein that forms 2-dimensional anticoagulant crystal shields ..
  7. Membrane Binding and Imaging Applications of Annexins
    Jonathan Tait; Fiscal Year: 2007
    ..Determine which mutant proteins have faster removal from the target site, thus allowing for repeated measurement of PS expression over short periods of time (up to one day). ..
  8. CO POISONING IN THE CONTEXT OF A REPERFUSION INJURY
    Stephen Thom; Fiscal Year: 2003
    ..The research plan is aimed to test the hypothesis that immune responses and the associated oxidative stress cause neurological injuries. ..
  9. RADIOIMMUNOTHERAPY OF B CELL LYMPHOMA WITH ANTI CD74
    M Mattes; Fiscal Year: 2002
    ..This study may lead to the development of more effective radio-conjugates for the therapy of cancer and other diseases. Also, it will provide basic information about the toxic effects of intracellular (really lysosomal) radionuclides. ..
  10. Amplification of TRIAL-Indcued Apoptosis in Mesothelioma
    V Broaddus; Fiscal Year: 2006
    ..the mechanisms of amplification of TRAIL-induced apoptosis can provide insight into the means by which malignant cells evade apoptosis and ways to bypass those sites of resistance and thereby improve treatment strategies ..
  11. PROTECTIVE ROLE OF APOPTOSIS IN ASBESTOS PLEURAL INJURY
    V Broaddus; Fiscal Year: 2001
    ..These studies should provide important information about the mechanisms by which asbestos induces apoptosis in mesothelial cells and the role of asbestos-induced apoptosis in asbestos-induced pathology. ..
  12. ADVANCES IN OPTICS FOR BIOTECHNOLOGY, MEDICINE
    Vasilis Ntziachristos; Fiscal Year: 2005
    ..Aim 4: To promote interaction between the invited scientists and participating students, to support education and understanding of the multi-disciplinary field of bio-optics and associated applications and to enable collaborations. ..
  13. Steroid As Cytoprotectants against Oxidative Toxicity
    Qin Chen; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  14. Plasmid Delivery & Expression in Embryonic Eye Tissues
    Kathy Svoboda; Fiscal Year: 2005
    ..This project has a high risk because neither "two-in-one" vectors, nor transfection into whole tissues has been accomplished. ..
  15. Akt Inhibitors to Treat Ewing's Sarcoma
    Jeffrey Toretsky; Fiscal Year: 2005
    ..Our findings from this Phase I Sun will hopefully lead to a key compound that can be further developed in a Phase II STTR ultimately leading to clinical trials in patients with ESFT. ..
  16. Ras/Raf & PI3K/Akt Induced Breast Cancer Drug Resistance
    JAMES MC CUBREY; Fiscal Year: 2007
    ..Throughthese studies,moreinformationwillbe availableto treatbreast andothercancerpatientswithcombinationsof drugs,whichinhibitsignaltransductionand anti-apoptoticpathwaysleadingtodrug resistance. ..
  17. Maturation dependent neuroprotection against alcohol
    Bahri Karacay; Fiscal Year: 2006
    ..The reagents and results derived from these in vitro experiments will lay the foundation for the gene therapy studies planned for in vivo applications in the future. ..
  18. Proteasome Inhibition in Myelodysplastic Syndromes
    Jane Liesveld; Fiscal Year: 2006
    ..These studies will define a potential therapeutic role for bortezomib in MDS and will aid in understanding the hematopoietic stem cell in these disorders. ..
  19. ICP-MS Instrument for Baylor College of Dentistry
    Kathy Svoboda; Fiscal Year: 2006
    ..We have identified two laboratories in the Dallas area that have appropriate instruments, but they cannot analyze our samples (see correspondence). [unreadable] [unreadable]..
  20. Tissue Factor-factor Vlla signaling
    Usha Pendurthi; Fiscal Year: 2010
    ..The proposed studies will provide valuable information in understanding how TF contributes to tumorigenesis. These studies will also provide clues on potential therapeutic targets for prevention of cancer progression. ..
  21. Pulmonary Mitochondrial Injury Caused by Tetrahydrocannabinol
    Theodore Sarafian; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  22. Translational Control of Oxidative Stress in Myocardial Infarction
    Qin M Chen; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: This grant proposes to study the mechanism of Nrf2 protein translation in cardiomyocytes and in the myocardium following oxidative stress. ..
  23. Activation of clotting & cell adhesion: gas embolism
    David M Eckmann; Fiscal Year: 2010
    ..This work is the basis for neuroprotection in gas embolism-induced stroke, a persistent, growing health threat without treatment. ..
  24. SULFOTRANSFERASE EXPRESSION IN EPITHELIA AND CARCINOMAS
    THOMAS DOOLEY; Fiscal Year: 2001
    ..The anticipated results will provide valuable insights into the roles of the human ST gene superfamily in the metabolism and bioavailability of drugs and endogenous compounds in human epithelia and cancers. ..
  25. Mechanism of Nicotine Action in the Healing Skeleton
    Michael Zuscik; Fiscal Year: 2009
    ....
  26. Reconstitution of Protective CMV Immunity and Immune Regulation After HAART
    Adriana Weinberg; Fiscal Year: 2008
    ..These analyses will define the role of TREG in the inadequate CMV immune reconstitution of HAART recipients. [unreadable] [unreadable] [unreadable] [unreadable]..
  27. Predictive Markers of Glioblastoma Response to VEGF Trap
    John de Groot; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  28. Creation and characterization of GH binding protein gene disrupted mice
    John Kopchick; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  29. Chemoprevention of Oral Cancer with BBIC
    Frank Meyskens; Fiscal Year: 2007
    ..abstract_text> ..
  30. Hybrid optical-ultrasound scanner for cancer imaging
    Vasilis Ntziachristos; Fiscal Year: 2005
    ..abstract_text> ..
  31. ESTROGEN STATUS AND THE FUNCTION OF CORONARY ARTERIES
    VIRGINIA MILLER; Fiscal Year: 2002
    ..The experiments of this proposal take such an approach using molecular to integrated whole animal physiology. ..
  32. STRESS RESPONSES IN MAMMALIAN CELLS
    Yong Lee; Fiscal Year: 2001
    ....
  33. Mechanisms of Pro-Thrombosis in Diabetes Mellitus
    David Schneider; Fiscal Year: 2003
    ..Further, these results will define the importance of prothrombotic potential in diabetic subjects while potentially identifying new therapeutic targets in patients with diabetes and other insulin resistant states. ..
  34. Imaging and Laser Revascularization in Hibernation
    Lynne Johnson; Fiscal Year: 2003
    ....
  35. BIOLOGY OF THE EMBRYONIC CORNEAL EPITHELIUM
    Kathy Svoboda; Fiscal Year: 2001
    ..In summary, these experiments in our well defined corneal epithelial model will focus on how ECM mediated signal transduction stimulates actin bundle formation and also save the cells from programmed cell death. ..
  36. A Novel HOX Gene Target in Breast Cancer
    PATRICIA BERG; Fiscal Year: 2003
    ..This study will therefore combine clinical and genetic approaches to determine the importance of BP1 expression in breast cancer. ..
  37. MICROSPECT IN TUMOR IMAGING
    HARRY STRAUSS; Fiscal Year: 2003
    ..At SKI, an ideal setting for such an instrument, the microSPECT will be an invaluable adjunct to the rapidly expanding small-animal imaging program. ..
  38. NEUTROPHINS AND RETINAL DEVELOPMENT
    Janet Sparrow; Fiscal Year: 2001
    ..The use of neurotrophins in conjunction with gene therapy or transplantation may also have potential for reversing retinal degenerative changes and lead to the restoration of vision. ..
  39. STRUCTURE/FUNCTION OF TRK RECEPTORS FOR NEUROTROPHINS
    KENNETH NEET; Fiscal Year: 2001
    ....
  40. CALCIUM DEPENDENT MEMBRANE BINDING PROTEINS
    CARL CREUTZ; Fiscal Year: 2002
    ....
  41. BP1, A HOMEOBOX GENE, IS OVER EXPRESSED IN BREAST CANCER
    PATRICIA BERG; Fiscal Year: 2002
    ..This multi-parameter study will therefore determine the clinical relevance of BP1 expression breast cancer. ..
  42. 5 LIPOXYGENASE IN BONE
    Lynda Bonewald; Fiscal Year: 2001
    ..Hence, these studies may also have profound implications for the increasing number of asthmatic patients who are currently being treated with steroids and 5LO inhibitors. ..
  43. SICKLE RBC, INTERMITTENT HYPOXIA AND VASCULAR PATHOLOGY
    FRANS KUYPERS; Fiscal Year: 2003
    ..Together, our results may indicate novel treatment regiments in the management of SCD. ..
  44. Contrast Enhanced Ultrasound with Therapeutic Modality
    Margaret Wheatley; Fiscal Year: 2004
    ..These studies are a prelude to show feasibility prior to in vivo studies. ..
  45. AUTOIMMUNE MECHANISMS OF THROMBOSIS
    Robert Roubey; Fiscal Year: 2004
    ..These data will be useful in focusing future research efforts on specific pathways of antibody-mediated monocyte activation and hypercoagulability and in designing new therapeutic strategies...
  46. Formation and Function of Human CD28-Negative T Cells
    Dorothy Lewis; Fiscal Year: 2004
    ..These studies are important for understanding the biology of human memory T cells especially for vaccine design. ..
  47. VIRUS PHOTOINACTIVATION--DIMETHYLMETHYLENE BLUE IN RBC
    Stephen Wagner; Fiscal Year: 2004
    ..abstract_text> ..
  48. Enhancement of Ad-p53 Therapy with Intratumoral Taxane
    GEORGE YOO; Fiscal Year: 2004
    ..Favorable data from the proposed project will lead to the development of clinical trials using intratumoral and intra-wound injections of docetaxel and Ad-p53. ..
  49. BACTERIAL MODULATION OF LUNG INFLAMMATORY RESPONSE
    Thomas Russo; Fiscal Year: 2004
    ..g. hemolysin) and bacterially induced host response elements in directly mediating the pathogenesis of lung injury. ..
  50. In Vitro Cancer Prevention Biomarker Development
    Eugene Elmore; Fiscal Year: 2004
    ..Using cluster analysis, we will determine the global gene expression changes induced by each agent. The strength of the proposed approach lies in the high quality data that has already determined from the HEC Assay. ..
  51. FUNCTION AND STRUCTURE OF ANNEXIN
    James Hall; Fiscal Year: 2003
    ..2. Determin the topography and oligomerization state of annexin. 3. Investigate the channel-forming abilities of annexin. ..
  52. MICROCIRCULATORY PROTECTION IN LATE PRECONDITIONING
    Wei Wang; Fiscal Year: 2001
    ..The third series of experiments will determine if microvascular protection induced by adenosine, nitric oxide, protein kinase C activation, or by IP, is dependent on the activation of iNOS. ..
  53. Phase III Colon Cancer Prevention Trial
    Frank Meyskens; Fiscal Year: 2006
    ..The overall goal of our work is to develop safe combinations of chemoprevention agents that can be used in the practice setting and complement current surveillance efforts. ..
  54. PanAmerican Society for Pigment Cell Research (PASPCR)
    Frank Meyskens; Fiscal Year: 2004
    ..abstract_text> ..
  55. Nutrients and Prostate Cancer Prevention
    Yong Lee; Fiscal Year: 2007
    ..Relevance: We believe that the successful outcome of this study will support the development and clinical application of DATS for the chemoprevention of human prostate cancer. [unreadable] [unreadable] [unreadable]..
  56. NEUROTROPHIC FACTOR MODULATION OF CORNEAL ENDOTHELIUM
    Shay Whey Koh; Fiscal Year: 2009
    ..abstract_text> ..
  57. The role of selenoproteins in the etiology of cancer
    Philip Moos; Fiscal Year: 2008
    ..Does SelP have a novel function in the regulation of lipid hydroperoxides? These studies provide an integrated framework to understand how electrophiles and selenium/selenoproteins contribute to the etiology of cancer. ..
  58. MECHANISMS OF TRANSFORMATION OF HEMATOPOIETIC CELLS
    JAMES MC CUBREY; Fiscal Year: 2001
    ..An understanding of how these signal transduction and apoptotic pathways impinge upon one another will further our comprehension of the consequences of abnormal oncogene activation and tumor progression. ..
  59. ANTICANCER DRUG RESISTANCE BY BCL-2 ONCOGENE EXPRESSION
    Raymond Meyn; Fiscal Year: 2007
    ..abstract_text> ..
  60. Role of Glutaredoxin in Metabolic Oxidative Stress
    Yong Lee; Fiscal Year: 2007
    ..We believe that investigating the mechanisms of metabolic oxidative stress responses in tumor cells will provide insight into how tumor cells recognize metabolic oxidative stress and initiate signal transduction. ..
  61. Metabolic Oxidative Stress and TRAIL Cytotoxicity
    Yong Lee; Fiscal Year: 2007
    ..We believe that the successful outcome of this study will support the development and clinical application of TRAIL for the treatment of human cancer. ..