protein sorting signals

Summary

Summary: Amino acid sequences found in transported proteins that selectively guide the distribution of the proteins to specific cellular compartments.

Top Publications

  1. ncbi Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes
    A Krogh
    Center for Biological Sequence Analysis, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark
    J Mol Biol 305:567-80. 2001
  2. ncbi Improved prediction of signal peptides: SignalP 3.0
    Jannick Dyrløv Bendtsen
    Center for Biological Sequence Analysis, BioCentrum DTU, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Mol Biol 340:783-95. 2004
  3. ncbi Predicting subcellular localization of proteins based on their N-terminal amino acid sequence
    O Emanuelsson
    Stockholm Bioinformatics Center, Department of Biochemistry, Stockholm University, Stockholm, S 106 91, Sweden
    J Mol Biol 300:1005-16. 2000
  4. pmc WoLF PSORT: protein localization predictor
    Paul Horton
    Computational Biology Research Center, AIST, Tokyo, Japan
    Nucleic Acids Res 35:W585-7. 2007
  5. ncbi CRM1 is an export receptor for leucine-rich nuclear export signals
    M Fornerod
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1051-60. 1997
  6. ncbi Computational method to predict mitochondrially imported proteins and their targeting sequences
    M G Claros
    Laboratoire de Genetique Moleculaire, CNRS URA 1302, Ecole Normale Superieure, Paris, France or
    Eur J Biochem 241:779-86. 1996
  7. ncbi Prediction of peroxisomal targeting signal 1 containing proteins from amino acid sequence
    Georg Neuberger
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    J Mol Biol 328:581-92. 2003
  8. ncbi A combined transmembrane topology and signal peptide prediction method
    LUKAS KALL
    Center for Genomics and Bioinformatics, Karolinska Institutet, SE 17 177 Stockholm, Sweden
    J Mol Biol 338:1027-36. 2004
  9. pmc Accurate prediction of secreted substrates and identification of a conserved putative secretion signal for type III secretion systems
    Ram Samudrala
    Department of Microbiology, University of Washington, Seattle, Washington, United States of America
    PLoS Pathog 5:e1000375. 2009
  10. pmc PrediSi: prediction of signal peptides and their cleavage positions
    Karsten Hiller
    Institut fur Mikrobiologie, Technische Universitat Braunschweig, Spielmannstrasse 7, D 38106 Braunschweig, Germany
    Nucleic Acids Res 32:W375-9. 2004

Detail Information

Publications404 found, 100 shown here

  1. ncbi Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes
    A Krogh
    Center for Biological Sequence Analysis, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark
    J Mol Biol 305:567-80. 2001
    ..We discuss the possible relevance of this finding for our understanding of membrane protein assembly mechanisms. A TMHMM prediction service is available at http://www.cbs.dtu.dk/services/TMHMM/...
  2. ncbi Improved prediction of signal peptides: SignalP 3.0
    Jannick Dyrløv Bendtsen
    Center for Biological Sequence Analysis, BioCentrum DTU, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Mol Biol 340:783-95. 2004
    ..The new method has been benchmarked against other available methods. Predictions can be made at the publicly available web server..
  3. ncbi Predicting subcellular localization of proteins based on their N-terminal amino acid sequence
    O Emanuelsson
    Stockholm Bioinformatics Center, Department of Biochemistry, Stockholm University, Stockholm, S 106 91, Sweden
    J Mol Biol 300:1005-16. 2000
    ..TargetP is available as a web-server at http://www.cbs.dtu.dk/services/TargetP/...
  4. pmc WoLF PSORT: protein localization predictor
    Paul Horton
    Computational Biology Research Center, AIST, Tokyo, Japan
    Nucleic Acids Res 35:W585-7. 2007
    ..Taken together, this information allows a user to understand the evidence (or lack thereof) behind the predictions made for particular proteins. WoLF PSORT is available at wolfpsort.org...
  5. ncbi CRM1 is an export receptor for leucine-rich nuclear export signals
    M Fornerod
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1051-60. 1997
    ..We conclude that CRM1 is an export receptor for leucine-rich nuclear export signals and discuss a model for the role of RanGTP in CRM1 function and in nuclear export in general...
  6. ncbi Computational method to predict mitochondrially imported proteins and their targeting sequences
    M G Claros
    Laboratoire de Genetique Moleculaire, CNRS URA 1302, Ecole Normale Superieure, Paris, France or
    Eur J Biochem 241:779-86. 1996
    ..As a practical application, the number of unknown yeast open reading frames that might be mitochondrial proteins has been predicted, which revealed that many of them are clustered...
  7. ncbi Prediction of peroxisomal targeting signal 1 containing proteins from amino acid sequence
    Georg Neuberger
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    J Mol Biol 328:581-92. 2003
    ..The predicted presence of a PTS1 signal implies peroxisomal localisation in the absence of N-terminal targeting sequences such as the mitochondrial import signal...
  8. ncbi A combined transmembrane topology and signal peptide prediction method
    LUKAS KALL
    Center for Genomics and Bioinformatics, Karolinska Institutet, SE 17 177 Stockholm, Sweden
    J Mol Biol 338:1027-36. 2004
    ..The method is available at as well as at..
  9. pmc Accurate prediction of secreted substrates and identification of a conserved putative secretion signal for type III secretion systems
    Ram Samudrala
    Department of Microbiology, University of Washington, Seattle, Washington, United States of America
    PLoS Pathog 5:e1000375. 2009
    ..This approach provides a new way to identify secreted effectors in a broad range of pathogenic bacteria for further experimental characterization and provides insight into the nature of the type III secretion signal...
  10. pmc PrediSi: prediction of signal peptides and their cleavage positions
    Karsten Hiller
    Institut fur Mikrobiologie, Technische Universitat Braunschweig, Spielmannstrasse 7, D 38106 Braunschweig, Germany
    Nucleic Acids Res 32:W375-9. 2004
    ..PrediSi is accessible via a web interface. An extra Java package was designed for the integration of PrediSi into other software projects. The tool is freely available on the World Wide Web at http://www.predisi.de...
  11. ncbi Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli
    Tao Wu
    Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA
    Cell 121:235-45. 2005
    ..These genetic interactions suggest a role for YfgL, one of the lipoprotein components of the protein assembly complex, in a homeostatic control mechanism that coordinates the overall OM assembly process...
  12. pmc Characterization and prediction of protein nucleolar localization sequences
    Michelle S Scott
    Division of Biological Chemistry and Drug Discovery and Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Nucleic Acids Res 38:7388-99. 2010
    ..NoLSs are a prevalent type of targeting motif that is distinct from nuclear localization signals and that can be computationally predicted...
  13. ncbi Signals for sorting of transmembrane proteins to endosomes and lysosomes
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 72:395-447. 2003
    ..This complex array of signals and recognition proteins ensures the dynamic but accurate distribution of transmembrane proteins to different compartments of the endosomal-lysosomal system...
  14. pmc Transmembrane protein topology prediction using support vector machines
    Timothy Nugent
    Bioinformatics Group, Department of Computer Science, University College London, Gower Street, London, WC1E 6BT, UK
    BMC Bioinformatics 10:159. 2009
    ..In the absence of structural data, sequence-based prediction methods allow TM protein topology to be investigated...
  15. pmc Protein targeting and degradation are coupled for elimination of mislocalized proteins
    Tara Hessa
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 475:394-7. 2011
    ..We propose that such coupling allows the fast tracking of MLPs for degradation without futile engagement of the cytosolic folding machinery...
  16. ncbi Interaction of tyrosine-based sorting signals with clathrin-associated proteins
    H Ohno
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 269:1872-5. 1995
    ..The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery...
  17. pmc Structural analysis of a monomeric form of the twin-arginine leader peptide binding chaperone Escherichia coli DmsD
    Charles M Stevens
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada
    J Mol Biol 389:124-33. 2009
    ..coli DmsA and the most conserved regions on the surface of EcDmsD...
  18. ncbi PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization
    K Nakai
    Institute for Molecular and Cellular Biology, Osaka University, Japan
    Trends Biochem Sci 24:34-6. 1999
  19. ncbi Extensive feature detection of N-terminal protein sorting signals
    Hideo Bannai
    Human Genome Center, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo, 108 8639, Japan
    Bioinformatics 18:298-305. 2002
    ..We adopt an approach which consists of an extensive search for simple rules and various attributes which is partially guided by human intuition...
  20. ncbi The Tat protein export pathway
    B C Berks
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Mol Microbiol 35:260-74. 2000
    ..Here, we review recent progress on the characterization of the Tat system and critically discuss the structure and operation of this major new bacterial protein export pathway...
  21. pmc Advantages of combined transmembrane topology and signal peptide prediction--the Phobius web server
    LUKAS KALL
    Center for Genomics and Bioinformatics, Karolinska Institutet, S 17177 Stockholm, Sweden
    Nucleic Acids Res 35:W429-32. 2007
    ..We here present a web interface (http://phobius.cgb.ki.se and http://phobius.binf.ku.dk) to access Phobius...
  22. pmc Proteomics of protein secretion by Bacillus subtilis: separating the "secrets" of the secretome
    Harold Tjalsma
    Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, Kerklaan 30, 9751 NN Haren, The Netherlands
    Microbiol Mol Biol Rev 68:207-33. 2004
    ..subtilis and other gram-positive bacteria. Ultimately, such leads will serve to increase our understanding of virulence factor biogenesis in gram-positive pathogens, which is likely to be of high medical relevance...
  23. doi Prediction of subcellular locations of proteins: where to proceed?
    Kenichiro Imai
    Computational Biology Research Center, AIST, Tokyo, Japan
    Proteomics 10:3970-83. 2010
    ..Recent advances on the study of protein sorting signals will hopefully be incorporated into future prediction methods...
  24. pmc ABI domain-containing proteins contribute to surface protein display and cell division in Staphylococcus aureus
    Matthew B Frankel
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 78:238-52. 2010
    ....
  25. ncbi Sec- and Tat-mediated protein secretion across the bacterial cytoplasmic membrane--distinct translocases and mechanisms
    Paolo Natale
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Biochim Biophys Acta 1778:1735-56. 2008
    ..Although the targeting signals that direct secretory proteins to these pathways show a high degree of similarity, the translocation mechanisms and translocases involved are vastly different...
  26. doi Features of a twin-arginine signal peptide required for recognition by a Tat proofreading chaperone
    Grant Buchanan
    Division of Molecular and Environmental Microbiology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom
    FEBS Lett 582:3979-84. 2008
    ..Screening of a random torD mutant library identified a variant TorD protein (Q7L) that displayed increased binding affinity for the TorA signal peptide...
  27. ncbi Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
    S K Mazmanian
    Department of Microbiology and Immunology, UCLA School of Medicine, University of California, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
    Science 285:760-3. 1999
    ..The protein specified by srtA, sortase, may be a useful target for the development of new antimicrobial drugs...
  28. ncbi Differential interactions between a twin-arginine signal peptide and its translocase in Escherichia coli
    Meriem Alami
    Institut für Biochemie und Molekularbiologie and Universität Freiburg, Hermann Herder Strasse 7, D 79104, Freiburg, Germany
    Mol Cell 12:937-46. 2003
    ....
  29. pmc A database of bacterial lipoproteins (DOLOP) with functional assignments to predicted lipoproteins
    M Madan Babu
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    J Bacteriol 188:2761-73. 2006
    ..The website, along with additional information on the biosynthetic pathway, statistics on predicted lipoproteins, and related figures, is available at http://www.mrc-lmb.cam.ac.uk/genomes/dolop/...
  30. ncbi A substrate-specific inhibitor of protein translocation into the endoplasmic reticulum
    Jennifer L Garrison
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94107, USA
    Nature 436:285-9. 2005
    ..Thus, the range of substrates accommodated by the channel can be specifically and reversibly modulated by a cell-permeable small molecule that alters the interaction between signal sequences and the Sec61 complex...
  31. ncbi Signal-CF: a subsite-coupled and window-fusing approach for predicting signal peptides
    Kuo Chen Chou
    Gordon Life Science Institute, 13784 Torrey Del Mar Drive, San Diego, CA 92130, USA
    Biochem Biophys Res Commun 357:633-40. 2007
    ..Signal-CF is freely available as a web-server at http://chou.med.harvard.edu/bioinf/Signal-CF/ or http://202.120.37.186/bioinf/Signal-CF/...
  32. ncbi A proteomic view on genome-based signal peptide predictions
    H Antelmann
    Institut fur Mikrobiologie und Molekularbiologie, Ernst Moritz Arndt Universiät Greifswald, D 17487 Greifswald, Germany
    Genome Res 11:1484-502. 2001
    ..Major problems are currently encountered with the prediction of extracellular proteins lacking signal peptides (including cytoplasmic proteins) and lipoproteins...
  33. pmc Prediction of lipoprotein signal peptides in Gram-negative bacteria
    Agnieszka S Juncker
    Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby 2800, Denmark
    Protein Sci 12:1652-62. 2003
    ..A neural network-based predictor was developed for comparison, and it gave very similar results. LipoP is available as a Web server at www.cbs.dtu.dk/services/LipoP/...
  34. doi Architects at the bacterial surface - sortases and the assembly of pili with isopeptide bonds
    Antoni P A Hendrickx
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Nat Rev Microbiol 9:166-76. 2011
    ..Together, these isopeptide bonds endow these sortase products with adhesive properties and resistance to host proteases...
  35. ncbi Motif refinement of the peroxisomal targeting signal 1 and evaluation of taxon-specific differences
    Georg Neuberger
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    J Mol Biol 328:567-79. 2003
    ..Specificity differences are confined to taxonomic subgroups (metazoa and fungi) and are connected with amino acid type preferences in region 1 and deviating hydrophobicity patterns in region 2...
  36. ncbi Signal peptidases
    Mark Paetzel
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Chem Rev 102:4549-80. 2002
  37. pmc Trafficking of STEVOR to the Maurer's clefts in Plasmodium falciparum-infected erythrocytes
    Jude M Przyborski
    Hygiene Institut, Abteilung Parasitologie, Universitatsklinikum Heidelberg, Heidelberg, Germany
    EMBO J 24:2306-17. 2005
    ..Our data suggest general principles for transport of transmembrane proteins to the Maurer's clefts and provide new insights into protein sorting and trafficking processes in P. falciparum...
  38. ncbi Locating proteins in the cell using TargetP, SignalP and related tools
    Olof Emanuelsson
    Stockholm Bioinformatics Center, AlbaNova, Stockholm University, SE 10691 Stockholm, Sweden
    Nat Protoc 2:953-71. 2007
    ..In addition, we describe and provide web references to other useful subcellular localization predictors. Finally, we discuss predictive performance measures in general and the performance of TargetP and SignalP in particular...
  39. pmc A new method for predicting signal sequence cleavage sites
    G von Heijne
    Nucleic Acids Res 14:4683-90. 1986
    ..The predictive accuracy is estimated to be around 75-80% for both prokaryotic and eukaryotic proteins...
  40. pmc The signal sequence coding region promotes nuclear export of mRNA
    Alexander F Palazzo
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Biol 5:e322. 2007
    ..The discovery of an SSCR-mediated pathway explains the previously noted amino acid bias in signal sequences and suggests a link between nuclear export and membrane targeting of mRNAs...
  41. ncbi Targeting malaria virulence and remodeling proteins to the host erythrocyte
    Matthias Marti
    Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    Science 306:1930-3. 2004
    ..The conservation of this signal across Plasmodium species has implications for the development of new antimalarials...
  42. ncbi A host-targeting signal in virulence proteins reveals a secretome in malarial infection
    N Luisa Hiller
    Departments of Pathology and Microbiology Immunology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Science 306:1934-7. 2004
    ..Erythrocyte modification by the parasite may involve plasmodial heat shock proteins and be vastly more complex than hitherto realized...
  43. pmc The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment
    Hilary F Clark
    Departments of Bioinformatics, Molecular Biology and Protein Chemistry, Genentech, Inc, South San Francisco, California 94080, USA
    Genome Res 13:2265-70. 2003
    ..The SPDI collection should facilitate efforts to better understand intercellular communication, may lead to new understandings of human diseases, and provides potential opportunities for the development of therapeutics...
  44. pmc An investigation of the role of transmembrane domains in Golgi protein retention
    S Munro
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 14:4695-704. 1995
    ..These observations are more consistent with lipid-based sorting of ST TMD, but they also allow for reconciliation with the kin recognition model which appears to act on sequences outside of the TMD...
  45. ncbi A novel and ubiquitous system for membrane targeting and secretion of cofactor-containing proteins
    J H Weiner
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Cell 93:93-101. 1998
    ..Proteins with a Sec-dependent leader or which assemble spontaneously in the membrane are not affected by this mutation. MttA, B, and C are members of a large family of related sequences extending from archaebacteria to higher eukaryotes...
  46. ncbi C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis
    Patricia A Digiuseppe Champion
    Department of Microbiology and Immunology, University of California, San Francisco, 600 16th Street, Campus Box 2200, San Francisco, CA 94143 2200, USA
    Science 313:1632-6. 2006
    ..Attachment of the signal to yeast ubiquitin was sufficient for secretion from M. tuberculosis cells, demonstrating that this ESX-1 signal is portable...
  47. pmc Exopolysaccharide-associated protein sorting in environmental organisms: the PEP-CTERM/EpsH system. Application of a novel phylogenetic profiling heuristic
    Daniel H Haft
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville MD 20850, USA
    BMC Biol 4:29. 2006
    ..Detection in some genomes, but not others, may reveal sorting system components by comparison of the phylogenetic profile of the class of sorting signal to that of various protein families...
  48. pmc A comprehensive assessment of N-terminal signal peptides prediction methods
    Khar Heng Choo
    Institute for Infocomm Research, 1 Fusionopolis Way, 21 01 Connexis, Singapore
    BMC Bioinformatics 10:S2. 2009
    ..Of the myriad software prediction tools developed to automate the task of assigning the SP cleavage site of these new sequences, we review here, the performance and reliability of commonly used SP prediction tools...
  49. pmc Conservation and diversification of dileucine signal recognition by adaptor protein (AP) complex variants
    Rafael Mattera
    Cell Biology and Metabolism Program, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 286:2022-30. 2011
    ..However, the characteristics of the binding site on each complex vary, providing for the specific recognition of a diverse repertoire of (D/E)XXXL(L/I) signals...
  50. ncbi Selective inhibition of cotranslational translocation of vascular cell adhesion molecule 1
    Jürgen Besemer
    Novartis Institutes for BioMedical Research, Brunner Strasse 59, A 1235 Vienna, Austria
    Nature 436:290-3. 2005
    ..In addition, they highlight cotranslational translocation at the ER membrane as a potential target for drug discovery...
  51. pmc The signal peptide of the rat corticotropin-releasing factor receptor 1 promotes receptor expression but is not essential for establishing a functional receptor
    Martina Alken
    Forschungsinstitut für Molekulare Pharmakologie FMP, Robert Rossle Str 10, 13125 Berlin, Germany
    Biochem J 390:455-64. 2005
    ..Thus the signal peptide of the CRF-R1 promotes an early step of receptor biogenesis, such as targeting of the nascent chain to the ER membrane and/or the gating of the protein-conducting translocon of the ER membrane...
  52. pmc Free-energy cost for translocon-assisted insertion of membrane proteins
    James Gumbart
    Department of Physics, Beckman Institute, University of Illinois at Urbana Champaign, Urbana Champaign, IL 61801, USA
    Proc Natl Acad Sci U S A 108:3596-601. 2011
    ....
  53. pmc Chloroplast transit peptide prediction: a peek inside the black box
    A I Schein
    University of Pennsylvania Department of Computer and Information Science, 556 Moore Building, 200 S 33rd Street, Philadelphia, PA 19104 6389, USA
    Nucleic Acids Res 29:E82. 2001
    ..Our most accurate predictor combines principal component analysis and logistic regression. Web-based prediction using this method is available online at http://apicoplast.cis.upenn.edu/pclr/...
  54. pmc Two proteases, trypsin domain-containing 1 (Tysnd1) and peroxisomal lon protease (PsLon), cooperatively regulate fatty acid β-oxidation in peroxisomal matrix
    Kanji Okumoto
    Department of Biology, Faculty of Sciences, Kyushu University Graduate School, 6 10 1 Hakozaki, Higashi ku, Fukuoka 812 8581, Japan
    J Biol Chem 286:44367-79. 2011
    ..The proteolytic activity of oligomeric Tysnd1 is in turn controlled by self-cleavage of Tysnd1 and degradation of Tysnd1 cleavage products by PsLon...
  55. pmc Genetic evidence for a tight cooperation of TatB and TatC during productive recognition of twin-arginine (Tat) signal peptides in Escherichia coli
    Frank Lausberg
    Institut für Bio und Geowissenschaften 1, Biotechnologie, Forschungszentrum Julich GmbH, Julich, Germany
    PLoS ONE 7:e39867. 2012
    ....
  56. ncbi How tails guide tail-anchored proteins to their destinations
    Nica Borgese
    National Research Council Institute for Neuroscience and Department of Medical Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy
    Curr Opin Cell Biol 19:368-75. 2007
    ..Unravelling these pathways will shed light on the biosynthesis and regulation of an important group of membrane proteins and is likely to lead to new concepts in the field of membrane biogenesis...
  57. pmc Cell wall antibiotics provoke accumulation of anchored mCherry in the cross wall of Staphylococcus aureus
    Wenqi Yu
    Microbial Genetics, University of Tubingen, Tubingen, Germany
    PLoS ONE 7:e30076. 2012
    ..The effect is most likely due to antibiotic mediated increase of free anchoring sites (Lipid II) at the cross wall, the substrate of SrtA, leading to a preferential incorporation of anchored proteins at the cross wall...
  58. doi Mechanisms of regulated unconventional protein secretion
    Walter Nickel
    Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nat Rev Mol Cell Biol 10:148-55. 2009
    ....
  59. pmc Widespread expression of BORIS/CTCFL in normal and cancer cells
    Tania A Jones
    Queen Mary University of London, Centre for Neuroscience and Trauma, Blizard Institute, Barts and the London School of Medicine and Dentistry, London, United Kingdom
    PLoS ONE 6:e22399. 2011
    ..While BORIS transcript levels are low compared to CTCF, its protein levels are readily detectable. These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function...
  60. doi Structure of a complex of the ATPase SecA and the protein-translocation channel
    Jochen Zimmer
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 455:936-43. 2008
    ..SecA binding generates a 'window' at the lateral gate of the SecY channel and it displaces the plug domain, preparing the channel for signal sequence binding and channel opening...
  61. pmc A signature in HIV-1 envelope leader peptide associated with transition from acute to chronic infection impacts envelope processing and infectivity
    Mohammed Asmal
    Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 6:e23673. 2011
    ..These results support the potential utility of using a computational approach to examine large viral sequence data sets for functional signatures and indicate the importance of Envelope expression levels for efficient HIV transmission...
  62. pmc A conserved tripeptide sorts proteins to peroxisomes
    S J Gould
    Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093
    J Cell Biol 108:1657-64. 1989
    ..These results indicate that peroxisomal protein import, unlike other types of transmembrane translocation, is dependent upon a conserved amino acid sequence...
  63. ncbi The thylakoid proton gradient promotes an advanced stage of signal peptide binding deep within the Tat pathway receptor complex
    Fabien Gérard
    Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 282:5263-72. 2007
    ..This implies that translocation involves a very intimate association of the signal peptide with the receptor complex binding site...
  64. pmc Dissection of the role of the stable signal peptide of the arenavirus envelope glycoprotein in membrane fusion
    Emily L Messina
    Montana Biotechnology Center, The University of Montana, Missoula, Montana, USA
    J Virol 86:6138-45. 2012
    ..Our studies suggest that interactions of SSP TM1 with the transmembrane domain of G2 may be important for GPC-mediated membrane fusion and its inhibition...
  65. pmc Interconvertibility of lipid- and translocon-bound forms of the bacterial Tat precursor pre-SufI
    Umesh K Bageshwar
    Department of Molecular and Cellular Medicine, College of Medicine, Texas A and M Health Science Center, College Station, TX 77843, USA
    Mol Microbiol 74:209-26. 2009
    ..Thus, the non-Tat bound form of the precursor is a functional intermediate in the transport cycle. This intermediate appears to directly equilibrate with the translocon-bound form of the precursor...
  66. pmc The curious case of arenavirus entry, and its inhibition
    Jack H Nunberg
    Montana Biotechnology Center, The University of Montana, Missoula, MT 59812, USA
    Viruses 4:83-101. 2012
    ..This unusual solution to maintaining the metastable prefusion state of GPC on the virion and activating the class I fusion cascade at acidic pH provides novel targets for antiviral intervention...
  67. pmc Genetic analysis of pathway specificity during posttranslational protein translocation across the Escherichia coli plasma membrane
    Natascha Blaudeck
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, D 52425 Julich, Germany
    J Bacteriol 185:2811-9. 2003
    ....
  68. pmc Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins
    K Ohishi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 11:1523-33. 2000
    ..Moreover, Gaa1p and Gpi8p are associated with each other. Therefore, Gaa1p and Gpi8p constitute a GPI transamidase and cooperate in generating a carbonyl intermediate, a prerequisite for GPI attachment...
  69. pmc Reverse genetics generation of chimeric infectious Junin/Lassa virus is dependent on interaction of homologous glycoprotein stable signal peptide and G2 cytoplasmic domains
    Cesar G Albarino
    Viral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 14, Atlanta, GA 30329, USA
    J Virol 85:112-22. 2011
    ..These recombinant chimeric viruses shed light on the fundamental requirements of arenavirus GPC maturation and may serve as a strategy for the development of bivalent JUNV and LASV vaccine candidates...
  70. pmc Assembly factor Omp85 recognizes its outer membrane protein substrates by a species-specific C-terminal motif
    Viviane Robert
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    PLoS Biol 4:e377. 2006
    ..coli outer membrane was accomplished after adapting its signature sequence. These results demonstrate that the Omp85 assembly machinery recognizes OMPs by virtue of their C-terminal signature sequence...
  71. pmc Structure of a zinc-binding domain in the Junin virus envelope glycoprotein
    Klára Briknarová
    Department of Chemistry and Biochemistry, University of Montana, Missoula, Montana 59812, USA
    J Biol Chem 286:1528-36. 2011
    ..Our studies describe the structural basis for retention of the unique SSP subunit and suggest a mechanism whereby SSP is positioned in the GPC complex to modulate pH-dependent membrane fusion...
  72. ncbi The yeast deubiquitinating enzyme Ubp16 is anchored to the outer mitochondrial membrane
    Andrea Kinner
    Institut fur Mikrobiologie, Heinrich Heine Universitat Dusseldorf, Geb 26 12 01, Universitatsstr 1, D 40225 Dusseldorf, Germany
    FEBS Lett 549:135-40. 2003
    ....
  73. pmc A multi-pronged search for a common structural motif in the secretion signal of Salmonella enterica serovar Typhimurium type III effector proteins
    Garry W Buchko
    Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    Mol Biosyst 6:2448-58. 2010
    ..Typhimurium effectors SptP, SopD-2, GtgE, and the Yersinia pestis effector YopH...
  74. doi TAT-pathway-dependent lipoproteins as a niche-based adaptation in prokaryotes
    Hamsanathan Shruthi
    Centre for Biotechnology, Anna University, Chennai 600 025, Tamil Nadu, India
    J Mol Evol 70:359-70. 2010
    ..The role of the TAT pathway in the export of folded-lipoproteins and in niche-specific adaptation for survival has important implications not only in lipoprotein biosynthesis, but also for protein and metabolic engineering applications...
  75. pmc Signal sequences specify the targeting route to the endoplasmic reticulum membrane
    D T Ng
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143 0448, USA
    J Cell Biol 134:269-78. 1996
    ..Our studies show a previously unrecognized diversity in ER-directed signal sequences, that carry structural information that serves to identify the route taken...
  76. pmc Design of a protein-targeting system for lactic acid bacteria
    Y Dieye
    , INRA, 78352 Jouy-en-Josas Cedex, France
    J Bacteriol 183:4157-66. 2001
    ..These results provide the first report of a targeting system in lactic acid bacteria in which the final location of a protein is controlled and biological activity is maintained...
  77. doi SPOCTOPUS: a combined predictor of signal peptides and membrane protein topology
    Håkan Viklund
    Department of Biochemistry and Biophysics, Center for Biomembrane Research, Stockholm Bioinformatics Center, The Arrhenius Laboratories for Natural Sciences, Stockholm University, SE 10691 Stockholm, Sweden
    Bioinformatics 24:2928-9. 2008
    ..We provide a description of the SPOCTOPUS algorithm together with a performance evaluation where SPOCTOPUS compares favorably with state-of-the-art methods for signal peptide and topology predictions...
  78. ncbi A cathepsin L isoform that is devoid of a signal peptide localizes to the nucleus in S phase and processes the CDP/Cux transcription factor
    Brigitte Goulet
    Department of Biochemistry, McGill University, 687 Pine Avenue West, Montreal H3A 1A1, Canada
    Mol Cell 14:207-19. 2004
    ..Overall, these results uncover an as yet unsuspected role for cysteine proteases in the control of cell cycle progression...
  79. pmc Peroxisomal membrane proteins contain common Pex19p-binding sites that are an integral part of their targeting signals
    Hanspeter Rottensteiner
    Institut fur Physiologische Chemie, Ruhr Universitat Bochum, 44780 Bochum, Germany
    Mol Biol Cell 15:3406-17. 2004
    ..By adding the heterologous binding site of Pex11p, peroxisomal targeting of the Pex13p fragment was restored. We conclude that Pex19p-binding sites are well-defined entities that represent an essential part of the mPTS...
  80. pmc Prediction of lipid posttranslational modifications and localization signals from protein sequences: big-Pi, NMT and PTS1
    Frank Eisenhaber
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Republic of Austria
    Nucleic Acids Res 31:3631-4. 2003
    ..Guidelines for application of the three WWW-based predictors (http://mendel.imp.univie.ac.at/) and for the interpretation of their output are described...
  81. pmc Unexpected deposition patterns of recombinant proteins in post-endoplasmic reticulum compartments of wheat endosperm
    Elsa Arcalis
    Institute for Molecular Biotechnology, Rheinische Westfälische Technische Hochschule Aachen, 52074 Aachen, Germany
    Plant Physiol 136:3457-66. 2004
    ..Our data also confirm that recombinant proteins are invaluable tools for the analysis of protein trafficking in cereals...
  82. pmc Secretion of anti-Plasmodium effector proteins from a natural Pantoea agglomerans isolate by using PelB and HlyA secretion signals
    Dawn C Bisi
    Duquesne University, Department of Biological Sciences, 600 Forbes Ave, Pittsburgh, PA 15282, USA
    Appl Environ Microbiol 77:4669-75. 2011
    ..Importantly, those strains that secreted efficiently grew as well as wild-type strains under laboratory conditions and, thus, may be expected to be competitive with the native microbiota in the environment of the mosquito midgut...
  83. pmc Sorting signals, N-terminal modifications and abundance of the chloroplast proteome
    Boris Zybailov
    Department of Plant Biology, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 3:e1994. 2008
    ..This study provides the most comprehensive chloroplast proteome analysis to date and an expanded Plant Proteome Database (PPDB) in which all MS data are projected on identified gene models...
  84. doi Remnant signal peptides on non-exported enzymes: implications for the evolution of prokaryotic respiratory chains
    Bérengère Ize
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Microbiology 155:3992-4004. 2009
    ..These data highlight an intimate genetic and evolutionary link between some non-exported redox enzymes and those transported across membranes by the Tat translocation system...
  85. ncbi An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle
    M A Poritz
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    Science 250:1111-7. 1990
    ..Protein synthesis did not cease until very late, an indication that 4.5S RNA probably does not have a direct role in this process...
  86. pmc Identification of Lassa virus glycoprotein signal peptide as a trans-acting maturation factor
    Robert Eichler
    Institut für Virologie der Philipps Universität Marburg, Robert Koch Strasse 17, D 35037 Marburg, Germany
    EMBO Rep 4:1084-8. 2003
    ..To our knowledge, this is the first report on a signal peptide that is essential for proteolytic processing of a secretory pathway protein...
  87. pmc A twin arginine signal peptide and the pH gradient trigger reversible assembly of the thylakoid [Delta]pH/Tat translocase
    Hiroki Mori
    Horticultural Sciences and Plant Molecular and Cellular Biology, University of Florida, Gainesville, FL 32611, USA
    J Cell Biol 157:205-10. 2002
    ..It also explains in part how the system can exist in the membrane without compromising its ion and proton permeability barrier...
  88. pmc Signal peptides direct surface proteins to two distinct envelope locations of Staphylococcus aureus
    Andrea DeDent
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    EMBO J 27:2656-68. 2008
    ....
  89. pmc Bitopic membrane topology of the stable signal peptide in the tripartite Junín virus GP-C envelope glycoprotein complex
    Sudhakar S Agnihothram
    Montana Biotechnology Center, The University of Montana, Science Complex, Room 221, Missoula, MT 59812, USA
    J Virol 81:4331-7. 2007
    ..Taken together, our results suggest that bitopic membrane insertion of SSP is centrally important in the assembly and function of the tripartite GP-C complex...
  90. ncbi Targeting of proteins derived from self-processing polyproteins containing multiple signal sequences
    Pablo de Felipe
    School of Biology, Centre for Biomolecular Sciences, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
    Traffic 5:616-26. 2004
    ..The inclusion of a fluorescent reporter enables visualisation of expression levels, whilst inclusion of a selectable marker enables stable cell-lines to be established rapidly...
  91. ncbi Protein translocation is mediated by oligomers of the SecY complex with one SecY copy forming the channel
    Andrew R Osborne
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 129:97-110. 2007
    ..Oligomeric channels with only one active pore likely mediate protein translocation in all organisms...
  92. doi 454 Genome sequencing of Pseudoperonospora cubensis reveals effector proteins with a QXLR translocation motif
    Miaoying Tian
    Department of Plant Pathology, Michigan State University, East Lansing, MI 48824, USA
    Mol Plant Microbe Interact 24:543-53. 2011
    ..cubensis. Furthermore, the massive duplication of PcQNE suggests that they might play pivotal roles in pathogen fitness and pathogenicity...
  93. pmc Sequence-based prediction of type III secreted proteins
    Roland Arnold
    Department of Genome Oriented Bioinformatics, Technische Universitat Munchen, Wissenschaftszentrum Weihenstephan, Freising, Germany
    PLoS Pathog 5:e1000376. 2009
    ..Our findings will facilitate further studies on and improve our understanding of type III secretion and its role in pathogen-host interactions...
  94. pmc Transmembrane topology and signal peptide prediction using dynamic bayesian networks
    Sheila M Reynolds
    Department of Electrical Engineering, University of Washington, Seattle, Washington, United States of America
    PLoS Comput Biol 4:e1000213. 2008
    ..gs.washington.edu/proj/philius. A Philius Web server is available at http://www.yeastrc.org/philius, and the predictions on the YRC database are available at http://www.yeastrc.org/pdr...
  95. pmc A particle-associated glycoprotein signal peptide essential for virus maturation and infectivity
    D Lindemann
    Institut fur Virologie und Immunbiologie, Universitat Wurzburg, Versbacher Str 7, 97078 Wurzburg, 01307 Dresden, Germany
    J Virol 75:5762-71. 2001
    ..While the SP N terminus was found to mediate the specificity of FV Env to interact with FV capsids, it was dispensable for Env targeting to the secretory pathway and FV envelope-mediated infectivity of murine leukemia virus pseudotypes...
  96. ncbi Nucleus-to-nucleus gene transfer and protein retargeting into a remnant cytoplasm of cryptophytes and diatoms
    Sven B Gould
    Philipps Universitat Marburg, Marburg, Germany
    Mol Biol Evol 23:2413-22. 2006
    ..tricornutum. Furthermore, the described cryptophyte PPC proteins possibly allow the elucidation of the processes by which proteins are involved in different levels of host control over its eukaryotic organelle...
  97. pmc Plasmepsin V licenses Plasmodium proteins for export into the host erythrocyte
    Ilaria Russo
    Howard Hughes Medical Institute, Washington University School of Medicine, Department of Molecular Microbiology, St Louis, Missouri 63110, USA
    Nature 463:632-6. 2010
    ..This enzyme is essential for parasite viability and ER residence is essential for its function. We propose that plasmepsin V is the PEXEL protease and is an attractive enzyme for antimalarial drug development...
  98. pmc Stepwise insertion and inversion of a type II signal anchor sequence in the ribosome-Sec61 translocon complex
    Prasanna K Devaraneni
    Department of Biochemistry and Molecular Biology, Oregon Health and Sciences University, Portland, OR 97239, USA
    Cell 146:134-47. 2011
    ..Thus, the RTC coordinates SA topogenesis within a protected environment via sequential energetic transitions of the TM segment...
  99. pmc Sequential checkpoints govern substrate selection during cotranslational protein targeting
    Xin Zhang
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Science 328:757-60. 2010
    ..Thus, high fidelity of substrate selection is achieved through the cumulative effect of multiple checkpoints; this principle may be generally applicable to other pathways involving selective signal recognition...
  100. ncbi A profile hidden Markov model for signal peptides generated by HMMER
    Zemin Zhang
    Department of Bioinformatics, Genentech Inc, South San Francisco, CA 94080, USA
    Bioinformatics 19:307-8. 2003
    ..Here we describe an approach for building a complex model of eukaryotic signal peptides by the standard HMMER package. Signal peptide prediction with this model gives a 95.6% sensitivity and 95.7% specificity...
  101. ncbi Plastid ultrastructure defines the protein import pathway in dinoflagellates
    Nasha Nassoury
    Departement de sciences biologiques, Universite de Montreal, 4101 Sherbrooke est, Montreal, Quebec, Canada H1X 2B2
    J Cell Sci 116:2867-74. 2003
    ..We conclude that the protein import mechanism is dictated by plastid ultrastructure rather than by the evolutionary history of the cell...

Research Grants65

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. The genetics and pathophysiology of impaired Wnt signaling in metabolic syndrome
    Arya Mani; Fiscal Year: 2012
    ..Moreover, we plan to screen number of families with inherited CAD, metabolic syndrome, and osteoporosis for mutations within this gene to identify novel mutations and to investigate their disease causing mechanisms. ..
  3. Regulation of LPS-mediated HMGB1 Release by Poly (ADP-ribose) Polymerase-1
    RAJESH K ANEJA; Fiscal Year: 2013
    ..Additional studies will be performed to determine the therapeutic window for use of PARP-1 inhibitors in murine sepsis. ..
  4. Characterization of new toxins (YmgD and YdfD) from E.coli, targeting cell wall
    Hisako Masuda; Fiscal Year: 2012
    ....
  5. Characterization of IL36, a novel cytokine
    Albert Zlotnik; Fiscal Year: 2012
    ..In this proposal, we aim to undertake its biological characterization, by exploring its effects on leukocytes in vitro as well as its function in vivo. ..
  6. Retroviral Subversion of ERAD and Intrinsic Immunity
    JAQUELIN PAGE DUDLEY; Fiscal Year: 2013
    ..Further understanding of ERAD and intrinsic immunity are essential to develop new treatments for cancer and pathogenic viral infections. ..
  7. Functional characterization of ATP1A1 and DEspR variants associated with essentia
    Nelson Ruiz Opazo; Fiscal Year: 2010
    ....
  8. Cthrc1 function as a novel TGF-beta antagonist in the vasculature
    Volkhard Lindner; Fiscal Year: 2013
    ..With its ability to inhibit collagen production, Cthrc1 could be very suitable for the development of future antifibrotic therapies. ..
  9. Nontoxigenic Clostridium difficile as an Oral Vaccine for C. difficile Infection
    DALE NICHOLAS GERDING; Fiscal Year: 2013
    ..abstract_text> ..
  10. Evaluation of In Vitro Companion Diagnostic Monoclonal Antibodies for Use in a St
    Christopher Horvath; Fiscal Year: 2013
    ..Combined use of anti-DEspR companion diagnostic and therapeutic mAbs has the potential to alter oncology clinical practice, particularly in BCa/TNBC, as an addition to or advantage over existing treatments. ..
  11. Identification and Function of nuDMP1 in Odontoblast Differentiation
    Yongbo Lu; Fiscal Year: 2013
    ....
  12. Nell-1, A Cbfa 1 Downstream Target, In Bone Formation
    Kang Ting; Fiscal Year: 2009
    ..A better understanding of how and why Nell-1 works to form bone can accelerate the development of clinical therapies to help patients grow better and faster bone. ..
  13. The Plasmodium 2TM and PHIST protein families
    Thomas J Templeton; Fiscal Year: 2012
    ..The expression, cellular localization, and phenotype following targeted gene disruption will be studied for select PHIST domain proteins toward an understanding of their function within the erythrocyte cytoplasm. ..
  14. PCSK9, Lipoprotein receptors, and Atherosclerosis
    Sergio Fazio; Fiscal Year: 2013
    ..We are proposing to investigate these correlations in detail and to evaluate the overall impact of PCSK9 expression on lipoprotein receptors and atherosclerosis. ..
  15. The accessory Sec system of Gram-positive pathogens
    Paul M Sullam; Fiscal Year: 2013
    ....
  16. Membrane Protein Folding and Assembly
    Stephen H White; Fiscal Year: 2013
    ..Our goal is to tighten the connection between the physical and biological principles as a basis for prediction algorithms. ..
  17. SURFACE PROTEIN ANCHORING IN GRAM-POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2013
    ..Appreciation of their molecular mechanisms and the structural as well as chemical nature of their inhibition will aid in the development of small molecules with therapeutic value for the treatment of bacterial infections. ..
  18. Illuminating the process of rod outer segment morphogenesis
    Yoshikazu Imanishi; Fiscal Year: 2013
    ..Understanding the process and mechanism of outer segment morphogenesis could reveal novel pathways which involve the products of ciliopathy causative genes. ..
  19. BIOCHEMISTRY AND REGULATION OF V(D)J RECOMBINATION
    Mark S Schlissel; Fiscal Year: 2012
    ..Our approach is to take what has been learned in vitro and use it to frame and test hypotheses regarding the regulation of recombination in vivo. ..
  20. Spatiotemporal Comparison of Aberrant & Ectopic VDJ Recombination Events In Vivo
    PENNY ELLEN SHOCKETT; Fiscal Year: 2010
    ....
  21. Effector and Regulatory Activities of HLA-E-restricted HIV-specific abCD8 T Cells
    June Kan-Mitchell; Fiscal Year: 2013
    ..These studies will provide insights into the role of HLA-E-restricted NK and CD8+ T cell surveillance in HIV infection. ..
  22. ANALYSIS AND ANNOTATION OF THE E. COLI GENOME SEQUENCE
    KENNETH EDWARD RUDD; Fiscal Year: 2012
    ..abstract_text> ..
  23. Converting an Oncogene to an Apoptotic Factor by Manipulating Signal Sequences
    Carol S Lim; Fiscal Year: 2012
    ..Our long-term, ultimate goal is to use localization controllable versions of Bcr-Abl (as gene therapy) for treatment of CML. ..
  24. Returning to the workforce supplement request for R01 GM095638
    Jennifer A Maynard; Fiscal Year: 2013
    ..abstract_text> ..
  25. THE THYROTHROPIN RECEPTOR IN HEALTH AND DISEASE
    Basil Rapoport; Fiscal Year: 2013
    ..The proposed studies on the least well understood TSHR NTD and hinge regions will provide novel insight into the structure and function of the TSH holoreceptor and its activation by TSH and TSAb in Graves'Disease. ..
  26. Retrocyclins: Circular Defensins Active Against HIV-1
    ALEXANDER MICHAEL COLE; Fiscal Year: 2011
    ..abstract_text> ..
  27. Role of VprBP in B cell development and V(D)J recombination
    Patrick C Swanson; Fiscal Year: 2013
    ....
  28. Plasmodium signal peptide peptidase as a novel antimalarial target
    Doron Greenbaum; Fiscal Year: 2009
    ..falciparum, we will utilize a multifaceted approach using biological, genetic and chemical techniques. ..
  29. mRNA Localization in Organelle Biogenesis
    Christopher V Nicchitta; Fiscal Year: 2013
    ..If successful, these studies will provide fundamental insights into the molecular principles of organelle biogenesis and support the development of therapeutic approaches to diseases of organelle dysfunction. ..
  30. Translational Control by Nutrients
    Maria Hatzoglou; Fiscal Year: 2010
    ..The proposed studies aim to identify these proteins and characterize how they activate the IRES. This is the first report on the regulation of the activity of the IRES by nutrients. ..
  31. Mitochondrial generation of superoxide in male reproduction
    Baisong Lu; Fiscal Year: 2010
    ..Data obtained from this project wiU improve our understanding of the effects of mitochondrial dysfunction on male reproductive and benefit the developing of new treatments for male infertilily. ..
  32. Investigation of FadA adhesin from Fusobacterium nucleatum
    Yiping Han; Fiscal Year: 2013
    ..nucleatum colonization in the host, and (ii) host components and pathways affected by FadA, which will facilitate modulation of respective cellular processes and targeted drug delivery. ..
  33. MOLECULAR MECHANISMS OF BACTERIAL PATHOGENESIS
    ROBERT JOHN COLLIER; Fiscal Year: 2012
    ..As with our past research, these studies are expected to yield findings relevant to uses of toxins in medical applications. ..
  34. Genetic Analysis of VDJ Recombination Regulation
    Cosmas C Giallourakis; Fiscal Year: 2010
    ..Understanding the cis-regulatory elements in VDJ recombination will enhance our understanding of lymphoid development and the control of gene expression in general. ..
  35. MECHANISM OF PROTEIN TRANSLOCATION ACROSS MEMBRANES
    Peter Walter; Fiscal Year: 2013
    ....
  36. Mechanism of membrane permeabilization by a cytolytic high density lipoprotein
    JOHN MATTHEW HARRINGTON; Fiscal Year: 2010
    ..Additionally, examining the role of the signal peptide will contribute to a fundamental understanding of the biology of HDL. ..
  37. CONTROL OF TCR DELTA AND TCR ALPHA REARRANGEMENT
    Michael S Krangel; Fiscal Year: 2013
    ..The hypothesis to be tested is that SATB1 is required for long distance interactions in the RAG locus that are critical for transcription of the RAG genes. ..
  38. Defining the role of a novel E3 ubiquitin ligase in V(D)J recombination
    Patrick C Swanson; Fiscal Year: 2011
    ..abstract_text> ..
  39. Granulin specific monoclonal antibodies to investigate their expression and role
    Ginette Serrero; Fiscal Year: 2013
    ..abstract_text> ..
  40. Modeling Pheromone Induced Plasmid and Drug Resistance Transfer
    Wei Shou Hu; Fiscal Year: 2013
    ..A better understanding of the mechanism of plasmid transfer will help us identify a new way to fight conjugative antibiotic resistance transfer. ..
  41. Chemical Address Tags: A Cheminformatics &Image Data Management and Analysis Pla
    Gus R Rosania; Fiscal Year: 2013
    ....
  42. Conformational Flexibility in Activation of Scr-Kinase
    Benoit Roux; Fiscal Year: 2013
    ....
  43. Improved Tools for Expression of Recombinant Genes in the Yeast Pichia pastoris
    JAMES MICHAEL CREGG; Fiscal Year: 2012
    ..At the initiation of this project, the head PI on the application, Dr. Cregg will "shift" from being a professor at the Keck Graduate Institute to an employee of Biogrammatics. ) ..
  44. Preproinsulin Translocation and Processing at the ER Membrane: a link to Diabetes
    Ming Liu; Fiscal Year: 2013
    ....
  45. The Role of gK in HSV-1 Induced Corneal Scarring
    Homayon Ghiasi; Fiscal Year: 2013
    ..Aim 2: Test the hypothesis that the 8-mer (ITAYGLVL) within the signal sequence of gK binds to MHC-I on DCs and mediates up-regulation of CD8+CD25+ T cells, thus leading to exacerbation of CS in ocularly infected mice. ..
  46. Structure-function studies of the tripartite Junin arenavirus GP-C
    Jack H Nunberg; Fiscal Year: 2012
    ..abstract_text> ..
  47. Mechanistic Role of NELL-1 in Premature Suture Closure
    Kang Ting; Fiscal Year: 2003
    ..Finally, we will integrate NELL-1 into the suture closure pathway with these known candidate genes. In the future, we will analyze NELL-1?s down-stream mechanism to further complete the premature closure mechanism in CS. ..
  48. SIGNAL PEPTIDASE STRUCTURE AND FUNCTION
    MARK LIVELY; Fiscal Year: 1999
    ..Achievement of this goal will permit precise determination of the mechanism of action of this unusual membrane- bound proteinase. ..
  49. Plant-produced Asialo-erythropoietin and its Antiapoptotic Functions
    Jiahua Xie; Fiscal Year: 2012
    ..In addition, the protein purification methodologies that will be established and the glycoprotein analysis methods will be very valuable for future plant glycoengineering projects. ..
  50. Substrate-Selective Inhibitors of Secretory and Membrane Protein Biogenesis
    Jack Taunton; Fiscal Year: 2010
    ..The major goal of this project is to design and synthesize new chemical tools that will allow us to dissect the molecular basis of cotransin's biological activity. cotransin ..
  51. HPMB AS A MODEL FOR HEMOLYSIN SECRETION/ACTIVATION
    TIMOTHY UPHOFF; Fiscal Year: 1999
    ..This information will serve as a model for similar proteins and permit optimization of this system for recombinant protein secretion from E. coli. ..
  52. Structural Biology of Intramembrane Proteolysis
    Yigong Shi; Fiscal Year: 2010
    ..This proposal seeks to understand the structures and mechanisms of these intramembrane proteases. ..
  53. FACTORS WHICH RECOGNIZE MAMMALIAN POLYADENYLATION SIGNAL
    Claire Moore; Fiscal Year: 1993
    ..This will be studied in vivo using transient expression assays, and if possible, with an in vitro system capable of transcription initiation and elongation, polyadenylation, and termination...
  54. NUCLEOCYTOPLASMIC TRAFFICKING
    David Goldfarb; Fiscal Year: 1992
    ..The pattern of lectin-labeled gold binding reveals the changing configuration of channel-rim proteins in response to this dilation. These studies will be completed and extended...
  55. FACTORS DETERMINING PROTEIN LOCALIZATION IN E. COLI
    JANNE CANNON; Fiscal Year: 1992
    ..6) In an effort to understand why some proteins are exported in a SecB-independent manner, we will expand our studies addressing the localization of the periplasmic ribose-binding protein...
  56. HEPATITIS B VIRUS GENE EXPRESSION
    Alan McLachlan; Fiscal Year: 1991
    ....
  57. CONTROL OF IMMUNOGLOBULIN SYNTHESIS
    Ursula Storb; Fiscal Year: 2001
    ..Understanding this mechanism will provide a fundamental basis for the unraveling of many diseases involving the immune system, such as autoimmunities, infectious diseases, allergies and cancer. ..
  58. SEQUENCING OF CLASS I MHC PEPTIDES BY MASS SPECTROMETRY
    Robert Cotter; Fiscal Year: 1999
    ..Finally, we describe two new projects that are now underway: the identification of MHC class I peptides associated with MMTV-infected mouse mammary tumor cells, and natural ligands recognized during T-cell selection in the thymus. ..
  59. BIOCHEMISTRY AND REGULATION OF V (D) J RECOMBINATION
    Mark Schlissel; Fiscal Year: 2004
    ....
  60. Analysis of Noelin, a Neural Crest Competence Factor
    Marianne Bronner Fraser; Fiscal Year: 2005
    ..3) Generate and characterize the phenotype of Noelin-1 null mutant. (4) Examine the environmental regulation of Noelin-1 expression. ..
  61. FURTHER STUDIES ON ANTI HIV 1 ACTIVITY OF INTERLEUKIN 16
    Paul Zhou; Fiscal Year: 2003
    ..TheserumlevelofIL-16andchemokinesandpotentialsignsof inflammation will be closely monitored. We hope these studies will lead to an IL-16- and chemokine-based therapeutic strategy for the treatment of AIDS. ..
  62. ATTENUATION CONTROL OF GENE EXPRESSION IN BACTERIA
    Charles Yanofsky; Fiscal Year: 1999
    ..In addition, attenuation in the B. subtilis trp operon may serve as a prokaryotic model for attenuation in higher organisms. ..
  63. MECHANISMS OF HEPATIC FATTY ACID TRANSPORT
    PAUL BERK; Fiscal Year: 2001
    ..These studies will help to explain how a mitochondrial matrix enzyme can serve as a plasma membrane transporter. ..
  64. MECHANISMS OF NUCLEAR PROTEIN ACCUMULATION
    PHILIP PAINE; Fiscal Year: 1993
    ....
  65. INTRINSIC SYSTEM CLOTTING: STRUCTURE AND FUNCTION
    Arthur Thompson; Fiscal Year: 1993
    ..A heavy chain defect due to a point mutation in a Taq1 cleavage site will be defined by oligonucleotide probes, to compare with functional data of the hemophilic mutation...