Alias: Vitreoscilla Pringsheim 1949

Top Publications

  1. Sutela S, Ylioja T, Jokipii Lukkari S, Anttila A, Julkunen Tiitto R, Niemi K, et al. The responses of Vitreoscilla hemoglobin-expressing hybrid aspen (Populus tremula × tremuloides) exposed to 24-h herbivory: expression of hemoglobin and stress-related genes in exposed and nonorthostichous leaves. J Plant Res. 2013;126:795-809 pubmed publisher
    ..Populus tremuloides Michx. expressing the hemoglobin (Hb) of Vitreoscilla (VHb) and non-transformant (wt) line. After 24-h exposure of leaves to Conistra vaccinii L...
  2. Chen Y, Xu H, Wang Y, Zhang J, Wang S. Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09. J Zhejiang Univ Sci B. 2014;15:979-85 pubmed publisher
    ..In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158...
  3. Suen Y, Tang H, Huang J, Chen F. Enhanced production of fatty acids and astaxanthin in Aurantiochytrium sp. by the expression of Vitreoscilla hemoglobin. J Agric Food Chem. 2014;62:12392-8 pubmed publisher
    ..The hemoglobin of the Vitreoscilla stercoraria (VHb) gene was fused upstream with a zeocin resistance gene (ble) and driven by the ..
  4. Gong T, Xu X, Dang Y, Kong A, Wu Y, Liang P, et al. An engineered Pseudomonas putida can simultaneously degrade organophosphates, pyrethroids and carbamates. Sci Total Environ. 2018;628-629:1258-1265 pubmed publisher
    ..putida KTUe within 15days. Moreover, Vitreoscilla hemoglobin (VHb)-expressing P. putida KTUe grew faster than P...
  5. Sun J, Raza M, Sun X, Yuan Q. Biosynthesis of adipic acid via microaerobic hydrogenation of cis,cis-muconic acid by oxygen-sensitive enoate reductase. J Biotechnol. 2018;280:49-54 pubmed publisher
    ..3?±?0.6?mg/L. To decrease the oxygen supply to downstream strains expressing CaER, Vitreoscilla hemoglobin (VHb) was introduced to upstream strains for its ability on oxygen obtaining...
  6. Lee W, Park E, Kim M. Enhanced production of ε-caprolactone by coexpression of bacterial hemoglobin gene in recombinant Escherichia coli expressing cyclohexanone monooxygenase gene. J Microbiol Biotechnol. 2014;24:1685-9 pubmed
    ..In this study, the bacterial hemoglobin gene from Vitreoscilla stercoraria (vhb) was introduced into the recombinant Escherichia coli expressing CHMO to investigate the ..
  7. Stark B, Pagilla K, Dikshit K. Recent applications of Vitreoscilla hemoglobin technology in bioproduct synthesis and bioremediation. Appl Microbiol Biotechnol. 2015;99:1627-36 pubmed publisher
    ..coli, engineering of heterologous hosts to express the hemoglobin from the bacterium Vitreoscilla (VHb) has become a widely used strategy to enhance production of a variety of bioproducts, stimulate ..
  8. Wu W, Guo X, Zhang M, Huang Q, Qi F, Huang J. Enhancement of  l-phenylalanine production in Escherichia coli by heterologous expression of Vitreoscilla hemoglobin. Biotechnol Appl Biochem. 2018;65:476-483 pubmed publisher
    ..However, the coexpression of Vitreoscilla hemoglobin gene (vgb), driven bya tac promoter, with the genes encoding 3-deoxy-d-arabinoheptulosonate-7-..
  9. Mora Lugo R, Madrigal M, Yelemane V, Fernandez Lahore M. Improved biomass and protein production in solid-state cultures of an Aspergillus sojae strain harboring the Vitreoscilla hemoglobin. Appl Microbiol Biotechnol. 2015;99:9699-708 pubmed publisher
    ..The bacterial Vitreoscilla hemoglobin (VHb) has favored the metabolism and productivities of various bacterial and yeast strains besides ..

More Information


  1. Li H, Zhang D, Yue T, Jiang L, Yu X, Zhao P, et al. Improved polysaccharide production in a submerged culture of Ganoderma lucidum by the heterologous expression of Vitreoscilla hemoglobin gene. J Biotechnol. 2016;217:132-7 pubmed publisher
    Expression of Vitreoscilla hemoglobin (VHb) gene was used to improve polysaccharide production in Ganoderma lucidum...
  2. Gu Y, Wang X, Yang C, Geng W, Feng J, Wang Y, et al. Effects of Chromosomal Integration of the Vitreoscilla Hemoglobin Gene (vgb) and S-Adenosylmethionine Synthetase Gene (metK) on ε-Poly-L-Lysine Synthesis in Streptomyces albulus NK660. Appl Biochem Biotechnol. 2016;178:1445-57 pubmed publisher
    Poly-L-lysine (ε-PL) is a widely used natural food preservative. To test the effects of the Vitreoscilla hemoglobin (VHb) and S-adenosylmethionine (SAM) on ε-PL synthesis in Streptomyces albulus NK660, the heterologous VHb gene ..
  3. Xu Z, Cao C, Sun Z, Li S, Xu Z, Feng X, et al. Construction of a Genetic System for Streptomyces albulus PD-1 and Improving Poly(ε-L-lysine) Production Through Expression of Vitreoscilla Hemoglobin. J Microbiol Biotechnol. 2015;25:1819-26 pubmed publisher
    ..Using the established genetic system, the Vitreoscilla hemoglobin (VHb) gene was integrated into the chromosome of S...
  4. Zhang W, Chen Z, Wu M, Shi Z, Zhu F, Li G, et al. Improved production of carotenoid-free welan gum in a genetic-engineered Alcaligenes sp. ATCC31555. Biotechnol Lett. 2016;38:991-7 pubmed publisher
    ..The vitreoscilla globin (vgb) gene combined with the β-galactosidase (lacZ) promoter was inserted into the phytoene synthase (..
  5. Mehrer C, Incha M, Politz M, Pfleger B. Anaerobic production of medium-chain fatty alcohols via a ?-reduction pathway. Metab Eng. 2018;48:63-71 pubmed publisher
    ..Next, we linked the anaerobically induced VHb promoter from Vitreoscilla hemoglobin to each gene to remove the need for chemical inducers and ensure robust expression...
  6. Wu H, Li J, Dong D, Liu T, Zhang T, Zhang D, et al. Heterologous coexpression of Vitreoscilla hemoglobin and Bacillus megaterium glucanase in Streptomyces lydicus A02 enhanced its production of antifungal metabolites. Enzyme Microb Technol. 2015;81:80-7 pubmed publisher
    ..lydicus A02, we coexpressed gene vgb encoding Vitreoscilla hemoglobin (VHb) and bglC encoding Bacillus megaterium L103 glucanase, both under the control of the strong ..
  7. Zhang Z, Li W, Li H, Zhang J, Zhang Y, Cao Y, et al. Construction and Characterization of Vitreoscilla Hemoglobin (VHb) with Enhanced Peroxidase Activity for Efficient Degradation of Textile Dye. J Microbiol Biotechnol. 2015;25:1433-41 pubmed publisher
    ..peroxidases are known for their ability to decolorize and detoxify textile dyes, the peroxidase activity of Vitreoscilla hemoglobin (VHb) has recently been studied...
  8. Sumer F, Stark B, Yesilcimen Akbas M. Efficient ethanol production from potato and corn processing industry waste using E. coli engineered to express Vitreoscilla haemoglobin. Environ Technol. 2015;36:2319-27 pubmed publisher
    Engineering of ethanologenic E. coli to express the haemoglobin (VHb) from the bacterium Vitreoscilla has been shown to enhance ethanol production by fermentation of pure sugars, sugars from hydrolysis of lignocellulose, components of ..
  9. Białkowska A, Jędrzejczak Krzepkowska M, Gromek E, Krysiak J, Sikora B, Kalinowska H, et al. Effects of genetic modifications and fermentation conditions on 2,3-butanediol production by alkaliphilic Bacillus subtilis. Appl Microbiol Biotechnol. 2016;100:2663-76 pubmed publisher
    ..LOCK 1086, constructed via different strategies such as cloning the gene encoding bacterial hemoglobin from Vitreoscilla stercoraria (vhb) and overexpression of the gene encoding acetoin reductase/2,3-butanediol dehydrogenase (bdhA) ..
  10. Du H, Shen X, Huang Y, Huang M, Zhang Z. Overexpression of Vitreoscilla hemoglobin increases waterlogging tolerance in Arabidopsis and maize. BMC Plant Biol. 2016;16:35 pubmed publisher
    b>Vitreoscilla hemoglobin (VHb) is a type of hemoglobin found in the Gram-negative aerobic bacterium Vitreoscilla that has been shown to contribute to the tolerance of anaerobic stress in multiple plant species. Maize (Zea mays L...
  11. He Y, Chen F, Sun M, Gao H, Guo Z, Lin H, et al. Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis. Molecules. 2018;23: pubmed publisher
    ..First, E. coli co-expressing (2R,3R)-2,3-butanediol dehydrogenase, NADH oxidase and Vitreoscilla hemoglobin was developed for (3S)-acetoin production from meso-2,3-butanediol...
  12. Kunkel S, Pagilla K, Stark B. Engineering of Nitrosomonas europaea to express Vitreoscilla hemoglobin enhances oxygen uptake and conversion of ammonia to nitrite. AMB Express. 2015;5:135 pubmed publisher
    ..transformed with a recombinant plasmid bearing the gene (vgb) encoding the hemoglobin (VHb) from the bacterium Vitreoscilla under control of the N. europaea amoC P1 promoter...
  13. Kunkel S, Pagilla K, Stark B. Directed evolution to produce sludge communities with improved oxygen uptake abilities. Appl Microbiol Biotechnol. 2015;99:10725-34 pubmed publisher
    ..The levels of heme b, the heme found in Vitreoscilla hemoglobin, increased in parallel to the increase in Hb-encoding species, to much higher levels in the low DO ..
  14. Juárez M, González De la Rosa C, Memún E, Sigala J, Lara A. Aerobic expression of Vitreoscilla hemoglobin improves the growth performance of CHO-K1 cells. Biotechnol J. 2017;12: pubmed publisher
    ..The expression of Vitreoscilla stercoraria hemoglobin (VHb) has been shown to significantly reduce overflow metabolism and improve the aerobic ..
  15. Zhu Y, Ye L, Chen Z, Hu W, Shi Y, Chen J, et al. Synergic regulation of redox potential and oxygen uptake to enhance production of coenzyme Q10 in Rhodobacter sphaeroides. Enzyme Microb Technol. 2017;101:36-43 pubmed publisher
    ..On the other hand, Vitreoscilla hemoglobin was heterologously expressed to enhance the oxygen uptake ability of the cells, leading to 127% ..
  16. Sar T, Seker G, Erman A, Stark B, Yesilcimen Akbas M. Repeated batch fermentation of immobilized E. coli expressing Vitreoscilla hemoglobin for long-term use. Bioengineered. 2017;8:651-660 pubmed publisher
    ..coli strains either expressing (TS3) or not expressing (FBR5) Vitreoscilla hemoglobin...
  17. Gong T, Xu X, Che Y, Liu R, Gao W, Zhao F, et al. Combinatorial metabolic engineering of Pseudomonas putida KT2440 for efficient mineralization of 1,2,3-trichloropropane. Sci Rep. 2017;7:7064 pubmed publisher
    ..we improved the oxygen sequestering capacity of this strain through the heterologous expression of Vitreoscilla hemoglobin, which makes this strain able to mineralize TCP under oxygen-limited conditions...
  18. Contreras S, Sagory Zalkind P, Blanquart H, Iltis A, Morand S. Complete Genome Sequence of Vitreoscilla filiformis (ATCC 15551), Used as a Cosmetic Ingredient. Genome Announc. 2017;5: pubmed publisher
    We report the first complete genome sequence of a Vitreoscilla filiformis strain (ATCC 15551) that is used in the cosmetic industry as Vitreoscilla ferment. The assembled genome consisted of one chromosome and two plasmids...
  19. Wang D, Liu L, Wang H, Xu H, Chen L, Ma L, et al. Clues for discovering a new biological function of Vitreoscilla hemoglobin in organisms: potential sulfide receptor and storage. FEBS Lett. 2016;590:1132-42 pubmed publisher
    The interaction between H2 S and Vitreoscilla hemoglobin (VHb) has been studied by UV-Vis and Resonance Raman spectroscopes to confirm the binding between the ligand and the protein. Kinetic constants, kon = 1...
  20. Zhao T, Li M, Zheng X, Wang C, Zhao H, Zhang C, et al. Improved production of trans-4-hydroxy-l-proline by chromosomal integration of the Vitreoscilla hemoglobin gene into recombinant Escherichia coli with expression of proline-4-hydroxylase. J Biosci Bioeng. 2017;123:109-115 pubmed publisher
    ..To solve this problem, the Vitreoscilla hemoglobin gene (vgb) was integrated into the chromosome of recombinant Escherichia coli expressing the P4H ..
  21. Breton A, Novikov A, Martin R, Tissieres P, Caroff M. Structural and biological characteristics of different forms of V. filiformis lipid A: use of MS to highlight structural discrepancies. J Lipid Res. 2017;58:543-552 pubmed publisher
    i>Vitreoscilla filiformis is a Gram-negative bacterium isolated from spa waters and described for its beneficial effects on the skin...
  22. Mu T, Yang M, Zhao J, Sharshar M, Tian J, Xing J. Improvement of desulfurizing activity of haloalkaliphilic Thialkalivibrio versutus SOB306 with the expression of Vitreoscilla hemoglobin gene. Biotechnol Lett. 2017;39:447-452 pubmed publisher
    ..transformation and expression system and further improve desulfurizing activity of cells through expression of Vitreoscilla hemoglobin (VHb) in haloalkaliphilic Thialkalivibrio versutus SOB306...
  23. Liu X, Zhu P, Jiang R, Wu L, Feng X, Li S, et al. Enhancement of welan gum production in Sphingomonas sp. HT-1 via heterologous expression of Vitreoscilla hemoglobin gene. Carbohydr Polym. 2017;156:135-142 pubmed publisher
    ..A strategy of heterologous expression of the Vitreoscilla hemoglobin gene in Sphingomonas sp...
  24. Sar T, Stark B, Yesilcimen Akbas M. Effective ethanol production from whey powder through immobilized E. coli expressing Vitreoscilla hemoglobin. Bioengineered. 2017;8:171-181 pubmed publisher
    ..coli and E. coli expressing Vitreoscilla hemoglobin (VHb) in both shake flask and fermenter cultures...
  25. Li H, He Y, Zhang D, Yue T, Jiang L, Li N, et al. Enhancement of ganoderic acid production by constitutively expressing Vitreoscilla hemoglobin gene in Ganoderma lucidum. J Biotechnol. 2016;227:35-40 pubmed publisher
    The Vitreoscilla hemoglobin (VHb) gene was expressed in Ganoderma lucidum to enhance antitumor ganoderic acid (GA) production...
  26. Zhang H, Feng Y, Cui Q, Song X. Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina. BMC Biotechnol. 2017;17:68 pubmed publisher
    ..Overexpression of the Vitreoscilla hemoglobin gene is thought to significantly increase the oxygen utilization efficiency of the cells...
  27. Lara A, Jaén K, Sigala J, Mühlmann M, Regestein L, Buchs J. Characterization of Endogenous and Reduced Promoters for Oxygen-Limited Processes Using Escherichia coli. ACS Synth Biol. 2017;6:344-356 pubmed publisher
    ..Here, we characterized the Vitreoscilla hemoglobin promoter (Pvgb) and a set of microaerobic endogenous promoters in Escherichia coli...
  28. Liu M, Li S, Xie Y, Jia S, Hou Y, Zou Y, et al. Enhanced bacterial cellulose production by Gluconacetobacter xylinus via expression of Vitreoscilla hemoglobin and oxygen tension regulation. Appl Microbiol Biotechnol. 2018;102:1155-1165 pubmed publisher
    ..In this study, the Vitreoscilla hemoglobin (VHb)-encoding gene vgb, which has been widely applied to improve cell survival during hypoxia, was ..
  29. Ouyang P, Wang H, Hajnal I, Wu Q, Guo Y, Chen G. Increasing oxygen availability for improving poly(3-hydroxybutyrate) production by Halomonas. Metab Eng. 2017;45:20-31 pubmed publisher
    ..Although the expression of bacterial Vitreoscilla hemoglobin (VHb) is useful for enhanced oxygen availability, bacterial cell membrane makes efficient hemoglobin-..
  30. Liu D, Wan N, Zhang F, Tang Y, Wu S. Enhancing fatty acid production in Escherichia coli by Vitreoscilla hemoglobin overexpression. Biotechnol Bioeng. 2017;114:463-467 pubmed publisher
    ..coli strains (after "Push-Pull-Block" based genetic modifications). To resolve this bottleneck, Vitreoscilla hemoglobin (VHb, a membrane protein facilitating O2 transport) was introduced into a fatty-acid-..
  31. Lin J, Zhang X, Song B, Xue W, Su X, Chen X, et al. Improving cellulase production in submerged fermentation by the expression of a Vitreoscilla hemoglobin in Trichoderma reesei. AMB Express. 2017;7:203 pubmed publisher
    ..reesei remains a major bottleneck that hampers growth and cellulase production in submerged fermentation. Vitreoscilla hemoglobin (VHb) has been demonstrated to improve metabolism and protein production in different heterologous ..
  32. He L, Yang H, Liu F, Chen Y, Tang S, Ji W, et al. Escherichia coli Nissle 1917 engineered to express Tum-5 can restrain murine melanoma growth. Oncotarget. 2017;8:85772-85782 pubmed publisher
    ..Subsequently, an anaerobic inducible expression vector was constructed under Vitreoscilla hemoglobin gene promoter Pvhb in E. coli Nissle 1917 (EcN)...
  33. Lara A, Jaén K, Sigala J, Regestein L, Buchs J. Evaluation of microbial globin promoters for oxygen-limited processes using Escherichia coli. J Biol Eng. 2017;11:39 pubmed publisher
    ..jejuni, Deinococcus radiodurans, Streptomyces coelicolor, Salmonella typhi and Vitreoscilla stercoraria were used to express the FMN-binding fluorescent protein (FbFP), which is a non-oxygen ..