Anaplasma phagocytophilum HZ

Summary

Alias: Anaplasma phagocytophilum str. HZ, Anaplasma phagocytophila HZ, Anaplasma phagocytophila str. HZ

Top Publications

  1. Lin Q, Zhang C, Rikihisa Y. Analysis of involvement of the RecF pathway in p44 recombination in Anaplasma phagocytophilum and in Escherichia coli by using a plasmid carrying the p44 expression and p44 donor loci. Infect Immun. 2006;74:2052-62 pubmed
    ..coli strain with active RecF recombination pathway but not in strains with deficient RecF pathway. Our results support the view that the p44 gene conversion in A. phagocytophilum occurs through the RecF pathway. ..
  2. Lin Q, Rikihisa Y, Massung R, Woldehiwet Z, Falco R. Polymorphism and transcription at the p44-1/p44-18 genomic locus in Anaplasma phagocytophilum strains from diverse geographic regions. Infect Immun. 2004;72:5574-81 pubmed
    ..These p44 loci provide insight into the molecular evolution and functional divergence of p44 paralogs and may serve as markers for typing strains from different geographic regions. ..
  3. Lee K, Padmalayam I, Baumstark B, Baker S, Massung R. Characterization of the ftsZ gene from Ehrlichia chaffeensis, Anaplasma phagocytophilum, and Rickettsia rickettsii, and use as a differential PCR target. DNA Cell Biol. 2003;22:179-86 pubmed
    ..phagocytophilum, 278 bp; E. chaffeensis, 341 bp; and Rickettsia spp., 425 bp. ..
  4. Seidman D, Hebert K, Truchan H, MILLER D, Tegels B, Marconi R, et al. Essential domains of Anaplasma phagocytophilum invasins utilized to infect mammalian host cells. PLoS Pathog. 2015;11:e1004669 pubmed publisher
    ..phagocytophilum binding and infection of host cells. This study dissects OmpA-receptor interactions and demonstrates the effectiveness of binding domain-specific antibodies for blocking A. phagocytophilum infection. ..
  5. Wang Y, Chen C, Zhang L. Molecular characterization of Msp2/P44 of Anaplasma phagocytophilum isolated from infected patients and Haemaphysalis longicornis in Laizhou Bay, Shandong Province, China. PLoS ONE. 2013;8:e78189 pubmed publisher
    ..phagocytophilum isolates might be relevant and might have serious clinical outcomes. This observation could provide a clue to further understand the pathogenesis of Chinese A. phagocytophilum. ..
  6. Kolbert C, Bruinsma E, Abdulkarim A, Hofmeister E, Tompkins R, Telford S, et al. Characterization of an immunoreactive protein from the agent of human granulocytic ehrlichiosis. J Clin Microbiol. 1997;35:1172-8 pubmed
    ..Further characterization of the sensitivity and specificity of immune responses to this protein will be facilitated by the use of recombinant fusion proteins or peptides based on the HGE agent-specific groEL homolog. ..
  7. Zhi N, Ohashi N, Rikihisa Y. Activation of a p44 pseudogene in Anaplasma phagocytophila by bacterial RNA splicing: a novel mechanism for post-transcriptional regulation of a multigene family encoding immunodominant major outer membrane proteins. Mol Microbiol. 2002;46:135-45 pubmed
    ..Collectively, the novel RNA splicing and the temperature-dependent transcription may account for the dominant p44-18 expression in mammals. ..
  8. Barbet A, Meeus P, B langer M, Bowie M, Yi J, Lundgren A, et al. Expression of multiple outer membrane protein sequence variants from a single genomic locus of Anaplasma phagocytophilum. Infect Immun. 2003;71:1706-18 pubmed
    ..marginale, A. phagocytophilum uses combinatorial mechanisms to generate a large array of outer membrane protein variants. Such gene polymorphism has profound implications for the design of vaccines, diagnostic tests, and therapy...
  9. Ojogun N, Kahlon A, Ragland S, Troese M, Mastronunzio J, Walker N, et al. Anaplasma phagocytophilum outer membrane protein A interacts with sialylated glycoproteins to promote infection of mammalian host cells. Infect Immun. 2012;80:3748-60 pubmed publisher
    ..Therefore, OmpA interacts with sialylated glycoproteins. This study identifies the first A. phagocytophilum adhesin-receptor pair and delineates the region of OmpA that is critical for infection...
  10. Chen G, Severo M, Sakhon O, Choy A, Herron M, Felsheim R, et al. Anaplasma phagocytophilum dihydrolipoamide dehydrogenase 1 affects host-derived immunopathology during microbial colonization. Infect Immun. 2012;80:3194-205 pubmed publisher
    ..Taken together, these findings suggest the presence of different signaling pathways in neutrophils and macrophages during A. phagocytophilum invasion and highlight the importance of LPDA1 as an immunopathological molecule...

Detail Information

Publications26

  1. Lin Q, Zhang C, Rikihisa Y. Analysis of involvement of the RecF pathway in p44 recombination in Anaplasma phagocytophilum and in Escherichia coli by using a plasmid carrying the p44 expression and p44 donor loci. Infect Immun. 2006;74:2052-62 pubmed
    ..coli strain with active RecF recombination pathway but not in strains with deficient RecF pathway. Our results support the view that the p44 gene conversion in A. phagocytophilum occurs through the RecF pathway. ..
  2. Lin Q, Rikihisa Y, Massung R, Woldehiwet Z, Falco R. Polymorphism and transcription at the p44-1/p44-18 genomic locus in Anaplasma phagocytophilum strains from diverse geographic regions. Infect Immun. 2004;72:5574-81 pubmed
    ..These p44 loci provide insight into the molecular evolution and functional divergence of p44 paralogs and may serve as markers for typing strains from different geographic regions. ..
  3. Lee K, Padmalayam I, Baumstark B, Baker S, Massung R. Characterization of the ftsZ gene from Ehrlichia chaffeensis, Anaplasma phagocytophilum, and Rickettsia rickettsii, and use as a differential PCR target. DNA Cell Biol. 2003;22:179-86 pubmed
    ..phagocytophilum, 278 bp; E. chaffeensis, 341 bp; and Rickettsia spp., 425 bp. ..
  4. Seidman D, Hebert K, Truchan H, MILLER D, Tegels B, Marconi R, et al. Essential domains of Anaplasma phagocytophilum invasins utilized to infect mammalian host cells. PLoS Pathog. 2015;11:e1004669 pubmed publisher
    ..phagocytophilum binding and infection of host cells. This study dissects OmpA-receptor interactions and demonstrates the effectiveness of binding domain-specific antibodies for blocking A. phagocytophilum infection. ..
  5. Wang Y, Chen C, Zhang L. Molecular characterization of Msp2/P44 of Anaplasma phagocytophilum isolated from infected patients and Haemaphysalis longicornis in Laizhou Bay, Shandong Province, China. PLoS ONE. 2013;8:e78189 pubmed publisher
    ..phagocytophilum isolates might be relevant and might have serious clinical outcomes. This observation could provide a clue to further understand the pathogenesis of Chinese A. phagocytophilum. ..
  6. Kolbert C, Bruinsma E, Abdulkarim A, Hofmeister E, Tompkins R, Telford S, et al. Characterization of an immunoreactive protein from the agent of human granulocytic ehrlichiosis. J Clin Microbiol. 1997;35:1172-8 pubmed
    ..Further characterization of the sensitivity and specificity of immune responses to this protein will be facilitated by the use of recombinant fusion proteins or peptides based on the HGE agent-specific groEL homolog. ..
  7. Zhi N, Ohashi N, Rikihisa Y. Activation of a p44 pseudogene in Anaplasma phagocytophila by bacterial RNA splicing: a novel mechanism for post-transcriptional regulation of a multigene family encoding immunodominant major outer membrane proteins. Mol Microbiol. 2002;46:135-45 pubmed
    ..Collectively, the novel RNA splicing and the temperature-dependent transcription may account for the dominant p44-18 expression in mammals. ..
  8. Barbet A, Meeus P, B langer M, Bowie M, Yi J, Lundgren A, et al. Expression of multiple outer membrane protein sequence variants from a single genomic locus of Anaplasma phagocytophilum. Infect Immun. 2003;71:1706-18 pubmed
    ..marginale, A. phagocytophilum uses combinatorial mechanisms to generate a large array of outer membrane protein variants. Such gene polymorphism has profound implications for the design of vaccines, diagnostic tests, and therapy...
  9. Ojogun N, Kahlon A, Ragland S, Troese M, Mastronunzio J, Walker N, et al. Anaplasma phagocytophilum outer membrane protein A interacts with sialylated glycoproteins to promote infection of mammalian host cells. Infect Immun. 2012;80:3748-60 pubmed publisher
    ..Therefore, OmpA interacts with sialylated glycoproteins. This study identifies the first A. phagocytophilum adhesin-receptor pair and delineates the region of OmpA that is critical for infection...
  10. Chen G, Severo M, Sakhon O, Choy A, Herron M, Felsheim R, et al. Anaplasma phagocytophilum dihydrolipoamide dehydrogenase 1 affects host-derived immunopathology during microbial colonization. Infect Immun. 2012;80:3194-205 pubmed publisher
    ..Taken together, these findings suggest the presence of different signaling pathways in neutrophils and macrophages during A. phagocytophilum invasion and highlight the importance of LPDA1 as an immunopathological molecule...
  11. Lin Q, Rikihisa Y, Ohashi N, Zhi N. Mechanisms of variable p44 expression by Anaplasma phagocytophilum. Infect Immun. 2003;71:5650-61 pubmed
    ..The data suggest that the P44 antigenic repertoire within the hypervariable region is restricted...
  12. Zhi N, Ohashi N, Tajima T, Mott J, Stich R, Grover D, et al. Transcript heterogeneity of the p44 multigene family in a human granulocytic ehrlichiosis agent transmitted by ticks. Infect Immun. 2002;70:1175-84 pubmed
    ..The present study provides new information for understanding the role of the p44 multigene family in transmission of the HGE agent between mammals and ticks...
  13. Caturegli P, Asanovich K, Walls J, Bakken J, Madigan J, Popov V, et al. ankA: an Ehrlichia phagocytophila group gene encoding a cytoplasmic protein antigen with ankyrin repeats. Infect Immun. 2000;68:5277-83 pubmed
    ..phagocytophila group bacteria and also detected antigen associated with chromatin in infected but not uninfected HL-60 cell cultures. These results suggest that this Ehrlichia protein may influence host cell gene expression...
  14. Massung R, Owens J, Ross D, Reed K, Petrovec M, Bjoersdorff A, et al. Sequence analysis of the ank gene of granulocytic ehrlichiae. J Clin Microbiol. 2000;38:2917-22 pubmed
  15. Zhi N, Ohashi N, Rikihisa Y. Multiple p44 genes encoding major outer membrane proteins are expressed in the human granulocytic ehrlichiosis agent. J Biol Chem. 1999;274:17828-36 pubmed
    ..Characterization of p44-homologous genes expressed by the HGE agent in a tissue culture would assist in understanding a role of the p44 multigene family in pathogenesis and immune response in HGE...
  16. Murphy C, Storey J, Recchia J, Doros Richert L, Gingrich Baker C, Munroe K, et al. Major antigenic proteins of the agent of human granulocytic ehrlichiosis are encoded by members of a multigene family. Infect Immun. 1998;66:3711-8 pubmed
    ..These data suggest that the 43- and 45-kDa proteins of the HGE agent are encoded by members of the GE MSP-2 multigene family...
  17. Zhi N, Ohashi N, Rikihisa Y, Horowitz H, Wormser G, Hechemy K. Cloning and expression of the 44-kilodalton major outer membrane protein gene of the human granulocytic ehrlichiosis agent and application of the recombinant protein to serodiagnosis. J Clin Microbiol. 1998;36:1666-73 pubmed
    ..The use of the affinity-purified rP44 protein as antigen would provide a more specific, consistent, and simpler serodiagnosis for HGE than the use of whole infected cells or purified HGE agents...
  18. Massung R, Lee K, Mauel M, Gusa A. Characterization of the rRNA genes of Ehrlichia chaffeensis and Anaplasma phagocytophila. DNA Cell Biol. 2002;21:587-96 pubmed publisher
    ..chaffeensis and A. phagocytophila. Phylogenetic analysis using the 23S rRNA gene suggests that reorganization of the phylum Proteobacteria by division of the class Alphaproteobacteria into two separate subclasses, may be appropriate...
  19. Inokuma H, Fujii K, Matsumoto K, Okuda M, Nakagome K, Kosugi R, et al. Demonstration of Anaplasma (Ehrlichia) platys inclusions in peripheral blood platelets of a dog in Japan. Vet Parasitol. 2002;110:145-52 pubmed
    ..The sequence analysis of the 16S rRNA, citrate synthase and heat shock protein genes of DNA from the infected platelets confirmed that the inclusions were A. platys. This is the first detection of A. platys inclusions in dogs in Japan...
  20. Inokuma H, Fujii K, Okuda M, Onishi T, Beaufils J, Raoult D, et al. Determination of the nucleotide sequences of heat shock operon groESL and the citrate synthase gene (gltA) of Anaplasma (Ehrlichia) platys for phylogenetic and diagnostic studies. Clin Diagn Lab Immunol. 2002;9:1132-6 pubmed
    ..The topology of the groEL- and gltA-based phylogenetic tree was similar to that derived from 16S rRNA gene analyses with distances. Both groESL- and gltA-based PCRs specific to A. platys were also developed based upon the alignment data...
  21. Von Loewenich F, Stumpf G, Baumgarten B, R llinghoff M, Dumler J, Bogdan C. A case of equine granulocytic ehrlichiosis provides molecular evidence for the presence of pathogenic anaplasma phagocytophilum (HGE agent) in Germany. Eur J Clin Microbiol Infect Dis. 2003;22:303-5 pubmed publisher
    ..Thus, the current lack of clinical cases of HGE in Germany is unlikely to result from the absence of pathogenic granulocytic ehrlichiae strains in German ticks...
  22. de la Fuente J, Massung R, Wong S, Chu F, Lutz H, Meli M, et al. Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains. J Clin Microbiol. 2005;43:1309-17 pubmed publisher
    ..These results suggest that A. phagocytophilum strains from ruminants could share some common characteristics, including reservoirs and pathogenicity, which may be different from strains that infect humans...
  23. Chae J, Foley J, Dumler J, Madigan J. Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic ehrlichiosis agent isolates from Northern California. J Clin Microbiol. 2000;38:1364-9 pubmed
    ..equi isolates from northern California. These results suggest that E. equi and the HGE agent found in California are similar or identical but may differ from the isolates of equine and human origin found in the eastern United States...
  24. Huang B, Troese M, Howe D, Ye S, Sims J, Heinzen R, et al. Anaplasma phagocytophilum APH_0032 is expressed late during infection and localizes to the pathogen-occupied vacuolar membrane. Microb Pathog. 2010;49:273-84 pubmed publisher
    ..phagocytophilum VirB/D T4SS effector, AnkA, but fails to secrete CyaA-tagged APH_0032 or APH_1387. These data confirm APH_0032 as an Anaplasma-derived AVM protein and hint that neither it nor APH_1387 are T4SS effectors...
  25. Storey J, Doros Richert L, Gingrich Baker C, Munroe K, Mather T, Coughlin R, et al. Molecular cloning and sequencing of three granulocytic Ehrlichia genes encoding high-molecular-weight immunoreactive proteins. Infect Immun. 1998;66:1356-63 pubmed
    ..This approach will be useful for identifying candidate diagnostic and vaccine antigens for granulocytic ehrlichiosis and aid in the classification of genogroup members...
  26. Taillardat Bisch A, Raoult D, Drancourt M. RNA polymerase beta-subunit-based phylogeny of Ehrlichia spp., Anaplasma spp., Neorickettsia spp. and Wolbachia pipientis. Int J Syst Evol Microbiol. 2003;53:455-8 pubmed publisher
    ..The rpoB nucleotide sequences were more variable than the 16S rRNA gene and groESL sequences at the species level...