Origins and Biological Consequences of Human Infertility

Summary

Principal Investigator: Linda C Giudice
Abstract: This renewal of our NIH U54 SCCPIR Center at the University of California, San Francisco proposes a transdisciplinary, team science approach to elucidate mechanisms underlying seminal events in early development as they relate to the origins and biological consequences of human infertility. Our investigators focus on the developmental continuum from oocyte competence to embryo and placental development to implantation and promote a shared vision of excellence and innovation in reproductive research, advanced technologies, informatics data analyses, mentoring, and community engagement. We combine powerful experimental techniques, system approaches and computational analyses to address critical questions at the intersection of stem cell biology, epigenomics, and clinical reproductive medicine. To accomplish these goals, we have proposed four highly interactive projects by established investigators and one pilot project with a new investigator, which are supported by three cores. Project I (Marco Conti) focuses on mechanisms controlling oocyte developmental competence and early embryo development;Project II (Susan Fisher) will investigate a molecular analysis of the early stages of trophoblast differentiation;Project III (Robert Blelloch) focuses on post-transcriptional regulation of trophoblast differentiation;Projec IV (Linda Giudice) will investigate development of human endometrium for embryonic implantation;and the Pilot Project (NamTran) proposes to investigate molecular mechanisms underlying the pathogenesis and pathophysiology of endometriosis, a common disorder in women associated with infertility and poor pregnancy outcomes These projects are supported by Core A for administration (Linda Giudice), Core B for computational biology research (Jun Song), and Core C for education and community outreach (Synthia Mellon). Our long-term goals are to understand mechanisms underlying normal and abnormal early development fundamentally and for developing novel strategies to diagnose, prevent and treat infertility;optimize fertility and pregnancy outcomes and minimize adverse outcomes;educate aspiring students, the public, health care professionals and patients about human development and disorders associated with abnormal development;and inspire career choice and nurture career development in this important area of reproductive health and research These goals are consistent with the mission of the NICHD.
Funding Period: 2007-05-03 - 2017-03-31
more information: NIH RePORT

Top Publications

  1. pmc Seminal plasma induces global transcriptomic changes associated with cell migration, proliferation and viability in endometrial epithelial cells and stromal fibroblasts
    Joseph C Chen
    Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA
    Hum Reprod 29:1255-70. 2014
  2. pmc In vitro culture of mouse embryos reduces differential gene expression between inner cell mass and trophectoderm
    G Giritharan
    Department of Obstetric and Gynecology, University of California, San Francisco, San Francisco, CA 94115, USA
    Reprod Sci 19:243-52. 2012
  3. pmc Krüppel-like factor 9 and progesterone receptor coregulation of decidualizing endometrial stromal cells: implications for the pathogenesis of endometriosis
    John Mark P Pabona
    Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, and the Arkansas Children s Nutrition Center, 15 Children s Way, Little Rock, Arkansas 72202, USA
    J Clin Endocrinol Metab 97:E376-92. 2012
  4. pmc Nuclear receptor, coregulator signaling, and chromatin remodeling pathways suggest involvement of the epigenome in the steroid hormone response of endometrium and abnormalities in endometriosis
    Z Zelenko
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA
    Reprod Sci 19:152-62. 2012
  5. pmc Novel signaling mechanisms in the ovary during oocyte maturation and ovulation
    Marco Conti
    Center for Reproductive Sciences, Department of Obstetrics and Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, United States
    Mol Cell Endocrinol 356:65-73. 2012
  6. pmc Perivascular human endometrial mesenchymal stem cells express pathways relevant to self-renewal, lineage specification, and functional phenotype
    Trimble L B Spitzer
    Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, USA
    Biol Reprod 86:58. 2012
  7. pmc Establishment of human trophoblast progenitor cell lines from the chorion
    Olga Genbacev
    Center for Reproductive Sciences, University of California San Francisco, San Francisco, California, USA
    Stem Cells 29:1427-36. 2011
  8. pmc A role for Notch signaling in trophoblast endovascular invasion and in the pathogenesis of pre-eclampsia
    Nathan M Hunkapiller
    Center for Reproductive Sciences, University of California San Francisco, CA 94143, USA
    Development 138:2987-98. 2011
  9. pmc From microRNAs to targets: pathway discovery in cell fate transitions
    Deepa Subramanyam
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Department of Urology, University of California San Francisco, San Francisco, CA, USA
    Curr Opin Genet Dev 21:498-503. 2011
  10. pmc Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition
    Jing Chen
    Center for Reproductive Sciences, University of California at San Francisco, San Francisco, California 94143, USA
    Genes Dev 25:755-66. 2011

Research Grants

Detail Information

Publications56

  1. pmc Seminal plasma induces global transcriptomic changes associated with cell migration, proliferation and viability in endometrial epithelial cells and stromal fibroblasts
    Joseph C Chen
    Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA
    Hum Reprod 29:1255-70. 2014
    ..How does seminal plasma (SP) affect the transcriptome of human primary endometrial epithelial cells (eEC) and stromal fibroblasts (eSF)?..
  2. pmc In vitro culture of mouse embryos reduces differential gene expression between inner cell mass and trophectoderm
    G Giritharan
    Department of Obstetric and Gynecology, University of California, San Francisco, San Francisco, CA 94115, USA
    Reprod Sci 19:243-52. 2012
    ..In summary, IVF and embryo culture significantly affects the transcriptome of ICM and TE cells...
  3. pmc Krüppel-like factor 9 and progesterone receptor coregulation of decidualizing endometrial stromal cells: implications for the pathogenesis of endometriosis
    John Mark P Pabona
    Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, and the Arkansas Children s Nutrition Center, 15 Children s Way, Little Rock, Arkansas 72202, USA
    J Clin Endocrinol Metab 97:E376-92. 2012
    ..Whether loss of KLF9 expression contributes to progesterone resistance of eutopic endometrium of women with endometriosis is unknown...
  4. pmc Nuclear receptor, coregulator signaling, and chromatin remodeling pathways suggest involvement of the epigenome in the steroid hormone response of endometrium and abnormalities in endometriosis
    Z Zelenko
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA
    Reprod Sci 19:152-62. 2012
    ..These data support the involvement of the epigenome in steroid hormone response of normal endometrium throughout the cycle and abnormalities in endometrium in women with endometriosis...
  5. pmc Novel signaling mechanisms in the ovary during oocyte maturation and ovulation
    Marco Conti
    Center for Reproductive Sciences, Department of Obstetrics and Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, United States
    Mol Cell Endocrinol 356:65-73. 2012
    ..This review will provide a summary of the current knowledge of the molecular events triggered by LH focusing mostly on the signaling pathways required for oocyte maturation...
  6. pmc Perivascular human endometrial mesenchymal stem cells express pathways relevant to self-renewal, lineage specification, and functional phenotype
    Trimble L B Spitzer
    Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, USA
    Biol Reprod 86:58. 2012
    ....
  7. pmc Establishment of human trophoblast progenitor cell lines from the chorion
    Olga Genbacev
    Center for Reproductive Sciences, University of California San Francisco, San Francisco, California, USA
    Stem Cells 29:1427-36. 2011
    ..These findings explain why CTBs of fully formed chorionic villi have a modest mitotic index and identify the chorionic mesoderm as a niche for TBPCs that support placental growth...
  8. pmc A role for Notch signaling in trophoblast endovascular invasion and in the pathogenesis of pre-eclampsia
    Nathan M Hunkapiller
    Center for Reproductive Sciences, University of California San Francisco, CA 94143, USA
    Development 138:2987-98. 2011
    ..In pre-eclampsia, expression of the Notch ligand JAG1 was absent in perivascular and endovascular TBs. We conclude that Notch signaling is crucial for TB vascular invasion...
  9. pmc From microRNAs to targets: pathway discovery in cell fate transitions
    Deepa Subramanyam
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Department of Urology, University of California San Francisco, San Francisco, CA, USA
    Curr Opin Genet Dev 21:498-503. 2011
    ..Here, we review these findings and discuss how miRNAs may be used as tools to discover novel pathways that are involved in cell fate transitions using dedifferentiation of somatic cells to induced pluripotent stem cells as a case study...
  10. pmc Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition
    Jing Chen
    Center for Reproductive Sciences, University of California at San Francisco, San Francisco, California 94143, USA
    Genes Dev 25:755-66. 2011
    ..Therefore, Cpeb1 and Dazl function in a progressive, self-reinforcing pathway to promote oocyte maturation and early embryonic development...
  11. pmc NANOS3 function in human germ cell development
    Vanessa T Angeles Julaton
    Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University, Palo Alto, CA 94305, USA
    Hum Mol Genet 20:2238-50. 2011
    ..Findings may contribute to our understanding of the basic biology of human germ cell development and may provide clinical insights regarding infertility...
  12. pmc Progesterone resistance in PCOS endometrium: a microarray analysis in clomiphene citrate-treated and artificial menstrual cycles
    Ricardo F Savaris
    Departamento de Ginecologia e Obstetricia, Programa de Pós Graduação em Cirurgia Ciências Cirúrgicas, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035 903, Brazil
    J Clin Endocrinol Metab 96:1737-46. 2011
    ..Polycystic ovary syndrome (PCOS), the most common endocrinopathy of reproductive-aged women, is characterized by ovulatory dysfunction and hyperandrogenism...
  13. pmc Biobanking human endometrial tissue and blood specimens: standard operating procedure and importance to reproductive biology research and diagnostic development
    Elizabeth Sheldon
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143, USA
    Fertil Steril 95:2120-2, 2122.e1-12. 2011
    ..To develop a standard operating procedure (SOP) for collection, transport, storage of human endometrial tissue and blood samples, subject and specimen annotation, and establishing sample priorities...
  14. pmc Pathogenesis and pathophysiology of endometriosis
    Richard O Burney
    Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Madigan Healthcare System, Tacoma, Washington, USA
    Fertil Steril 98:511-9. 2012
    ..A thorough understanding of the histopathogenesis and pathophysiology of endometriosis is essential to the development of novel diagnostic and treatment approaches for this debilitating condition...
  15. pmc Primordial germ cells and gastrointestinal stromal tumors respond distinctly to a cKit overactivating allele
    Li Chen
    Department of Obstetrics Gynecology and Reproductive Sciences, Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, CA 94143 0667, USA
    Hum Mol Genet 22:313-27. 2013
    ..These results demonstrate a cell-context-dependent response to the cKit(V558Δ) mutation. We propose that AKT overload protection and JNK-mediated survival comprise PGC-specific mechanisms for regulating cKit signaling...
  16. pmc Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes
    Jing Chen
    1 Center for Reproductive Sciences, University of California, San Francisco, California 94143, USA 2 Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, California 94143, USA 3 Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143, USA
    Nat Cell Biol 15:1415-23. 2013
    ..These somatic cell signals that affect translation require activation of the PI(3)K-AKT-mTOR pathway. Thus, mRNA translation depends on somatic cell cues that are essential to reprogramme the oocyte for embryo development. ..
  17. pmc MicroRNA-based discovery of barriers to dedifferentiation of fibroblasts to pluripotent stem cells
    Robert L Judson
    1 The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Program in Biomedical Sciences, University of California San Francisco, San Francisco, California, USA 2 Department of Urology, University of California San Francisco, San Francisco, California, USA
    Nat Struct Mol Biol 20:1227-35. 2013
    ..These data establish two of the largest functionally defined networks of miRNA-mRNA interactions and reveal previously unidentified relationships among genes that act together to suppress early stages of reprogramming. ..
  18. pmc The human oviduct transcriptome reveals an anti-inflammatory, anti-angiogenic, secretory and matrix-stable environment during embryo transit
    A P Hess
    Department of Gynecology and Obstetrics, Stanford University Medical School, CA, USA University of Dusseldorf, Medical Faculty, Department of Gynecology, Obstetrics and REI UniKiD, Dusseldorf, Germany
    Reprod Biomed Online 27:423-35. 2013
    ....
  19. ncbi Epigenetics of cellular reprogramming
    Raga Krishnakumar
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences and Department of Urology, University of California San Francisco, San Francisco, CA, USA
    Curr Opin Genet Dev 23:548-55. 2013
    ....
  20. pmc Histone variant H3.3 maintains a decondensed chromatin state essential for mouse preimplantation development
    Chih Jen Lin
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Obstetrics and Gynecology and Center for Reproductive Sciences, University of California San Francisco, 35 Medical Center Way, San Francisco, CA 94143, USA
    Development 140:3624-34. 2013
    ..These results reveal that H3.3 mediates a balance between open and condensed chromatin that is crucial for the fidelity of chromosome segregation during early mouse development. ..
  21. pmc Coculturing human endometrial epithelial cells and stromal fibroblasts alters cell-specific gene expression and cytokine production
    Joseph C Chen
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, Center for Reproductive Sciences, San Francisco, California
    Fertil Steril 100:1132-43. 2013
    ..To determine the effects of coculturing endometrial epithelial cells (eEC) with paired endometrial stromal fibroblasts (eSF) on cell-specific gene expression and cytokine secretion patterns...
  22. pmc miR-294/miR-302 promotes proliferation, suppresses G1-S restriction point, and inhibits ESC differentiation through separable mechanisms
    Yangming Wang
    Peking Tsinghua Joint Center for Life Sciences, Institute of Molecular Medicine, Peking University, Beijing 100871, China
    Cell Rep 4:99-109. 2013
    ..These results show that the miR-294/miR-302 family acts through Rb-dependent and -independent pathways to regulate the G1 restriction point and the silencing of self-renewal, respectively. ..
  23. pmc Mesenchymal stem/progenitors and other endometrial cell types from women with polycystic ovary syndrome (PCOS) display inflammatory and oncogenic potential
    T T Piltonen
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, 505 Parnassus Avenue, San Francisco, California 94143 0132, USA
    J Clin Endocrinol Metab 98:3765-75. 2013
    ..Endometrium in polycystic ovary syndrome (PCOS) presents altered gene expression indicating progesterone resistance and predisposing to reduced endometrial receptivity and endometrial cancer...
  24. pmc Bivalent chromatin marks developmental regulatory genes in the mouse embryonic germline in vivo
    Michael Sachs
    Biomedical Sciences Graduate Program, 35 Medical Center Way, University of California, San Francisco, CA 94143, USA
    Cell Rep 3:1777-84. 2013
    ..Our results demonstrate the existence of bivalent domains in the germline and raise the possibility that the somatic program is continuously maintained as bivalent, potentially imparting transgenerational epigenetic inheritance. ..
  25. ncbi Human trophoblast progenitors: where do they reside?
    Olga Genbacev
    Ely and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, CA 94143, USA
    Semin Reprod Med 31:56-61. 2013
    ..In turn, this information can be used to trace the origins of pregnancy complications that are associated with faulty TB growth and differentiation...
  26. ncbi Small RNAs in germline development
    Matthew S Cook
    Department of Urology, University of California, San Francisco, California, USA
    Curr Top Dev Biol 102:159-205. 2013
    ..Here, we review current knowledge of the roles miRNAs, endo-siRNAs, and piRNAs play at all stages of germline development in various organisms, focusing on studies in the mouse...
  27. pmc Changes in eutopic endometrial gene expression during the progression of experimental endometriosis in the baboon, Papio anubis
    Yalda Afshar
    Department of Obstetrics and Gynecology, Cedars Sinai Medical Center, Los Angeles, CA, USA
    Biol Reprod 88:44. 2013
    ..Furthermore, this baboon model provides a powerful means whereby the early events associated with the pathology of disease and the resulting infertility may be elucidated...
  28. pmc Effect of bisphenol A on human endometrial stromal fibroblasts in vitro
    L Aghajanova
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, CA 94143, USA
    Reprod Biomed Online 22:249-56. 2011
    ..028). BPA has an endocrine-disrupting effect on human ESF function and gene expression but the underlying mechanisms appear not to involve oestrogen-mediated pathways...
  29. pmc Trophoblast stem cells
    R Michael Roberts
    Division of Animal Sciences, Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA
    Biol Reprod 84:412-21. 2011
    ..Recent derivation of TSC from blastocysts of the rhesus monkey suggests that isolation of the human equivalents may be possible and will reveal the extent to which mechanisms uncovered by using animal models are true in our own species...
  30. pmc Transplantation directs oocyte maturation from embryonic stem cells and provides a therapeutic strategy for female infertility
    Cory R Nicholas
    Department of Obstetrics and Gynecology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Palo Alto, CA 94304, USA
    Hum Mol Genet 18:4376-89. 2009
    ....
  31. pmc MicroRNA expression profiling of eutopic secretory endometrium in women with versus without endometriosis
    R O Burney
    Division of Reproductive Endocrinology and Infertility, Madigan Army Medical Center, Tacoma, WA, USA
    Mol Hum Reprod 15:625-31. 2009
    ....
  32. pmc Increased mitogen-activated protein kinase kinase/extracellularly regulated kinase activity in human endometrial stromal fibroblasts of women with endometriosis reduces 3',5'-cyclic adenosine 5'-monophosphate inhibition of cyclin D1
    Michael C Velarde
    Department of Obstetrics, Center for Reproductive Sciences, University of California San Francisco, San Francisco, California 94143 0132, USA
    Endocrinology 150:4701-12. 2009
    ..These findings suggest that increased activation of ERK1/2 in endometrial cells from women with endometriosis may be responsible for persistent proliferative changes in secretory-phase endometrium...
  33. pmc Dazl functions in maintenance of pluripotency and genetic and epigenetic programs of differentiation in mouse primordial germ cells in vivo and in vitro
    Kelly M Haston
    Department of Obstetrics and Gynecology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Palo Alto, California, United States of America
    PLoS ONE 4:e5654. 2009
    ..Little is known of the genes that regulate quantitative and qualitative aspects of early mammalian germ cell development both in vivo, and during differentiation of germ cells from mouse embryonic stem cells (mESCs) in vitro...
  34. pmc The progesterone receptor coactivator Hic-5 is involved in the pathophysiology of endometriosis
    Lusine Aghajanova
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USA
    Endocrinology 150:3863-70. 2009
    ....
  35. pmc Instructing an embryonic stem cell-derived oocyte fate: lessons from endogenous oogenesis
    Cory R Nicholas
    Department of Obstetrics and Gynecology, Stanford University School of Medicine, Palo Alto, California 94304, USA
    Endocr Rev 30:264-83. 2009
    ..Thus, ESC-derived oocytes could ultimately help to restore fertility in women. Here, we review endogenous and ESC-derived oocyte development, and we discuss the potential and challenges for differentiating functional oocytes from ESCs...
  36. pmc Priorities for endometriosis research: recommendations from an international consensus workshop
    Peter A W Rogers
    Monash University, Melbourne, Australia
    Reprod Sci 16:335-46. 2009
    ....
  37. pmc Characterization of a Dazl-GFP germ cell-specific reporter
    Cory R Nicholas
    Department of Obstetrics and Gynecology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Palo Alto, CA, USA
    Genesis 47:74-84. 2009
    ....
  38. pmc Chapter 12. Placental remodeling of the uterine vasculature
    Nathan M Hunkapiller
    Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
    Methods Enzymol 445:281-302. 2008
    ..Finally, we conclude with methods devised by our group and others to explore the complex heterotypic cell-cell interactions that occur as CTBs invade blood vessels in vivo in the nude mouse...
  39. pmc Steroidogenic enzyme and key decidualization marker dysregulation in endometrial stromal cells from women with versus without endometriosis
    L Aghajanova
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143, USA
    Biol Reprod 80:105-14. 2009
    ....
  40. pmc VEGF blockade inhibits angiogenesis and reepithelialization of endometrium
    Xiujun Fan
    Department of Obstetrics and Gynecology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
    FASEB J 22:3571-80. 2008
    ..In summary, we provide novel experimental data indicating that VEGF is essential for endometrial neoangiogenesis during postmenstrual/postpartum repair...
  41. pmc Endometrial gene expression in early pregnancy: lessons from human ectopic pregnancy
    Ricardo F Savaris
    Departmento de Obstetrícia e Ginecologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
    Reprod Sci 15:797-816. 2008
    ..Validation was done with reverse transcriptase polymerase chain reaction. In conclusion, the ectopic embryo has a significant impact, by an endocrine mechanism, on endometrium, when compared with the window of implantation...
  42. ncbi Genomics analysis: endometrium
    Ricardo Francalacci Savaris
    Departamento de Ginecologia e Obstetrícia Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Methods Mol Biol 590:91-113. 2009
    ..The present study reviews these considerations and the principles of transcriptomic analysis through an example of a comparison of three different phases of the menstrual cycle...
  43. pmc Human DAZL, DAZ and BOULE genes modulate primordial germ-cell and haploid gamete formation
    Kehkooi Kee
    Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University, Palo Alto, California 94305, USA
    Nature 462:222-5. 2009
    ..These results are significant to the generation of gametes for future basic science and potential clinical applications...
  44. pmc Molecular evidence for differences in endometrium in severe versus mild endometriosis
    Lusine Aghajanova
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco UCSF, San Francisco, CA 94143, USA
    Reprod Sci 18:229-51. 2011
    ....
  45. pmc Effect of ICSI on gene expression and development of mouse preimplantation embryos
    G Giritharan
    Department of Obstetric, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, CA 94143, USA
    Hum Reprod 25:3012-24. 2010
    ..In vitro culture (IVC) and IVF of preimplantation mouse embryos are associated with changes in gene expression. It is however not known whether ICSI has additional effects on the transcriptome of mouse blastocysts...
  46. pmc Unique transcriptome, pathways, and networks in the human endometrial fibroblast response to progesterone in endometriosis
    L Aghajanova
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA
    Biol Reprod 84:801-15. 2011
    ..The abnormal response of this cell type to P(4) may contribute to compromised embryonic implantation and infertility in women with endometriosis...
  47. pmc Effect of the method of conception and embryo transfer procedure on mid-gestation placenta and fetal development in an IVF mouse model
    L Delle Piane
    Obstetric, Gynecology and Reproductive Science Division, University of California San Francisco, San Francisco, CA, USA
    Hum Reprod 25:2039-46. 2010
    ..This study evaluates, in a mouse model, whether the method of conception and embryo transfer affect placentation and fetal development...
  48. pmc The placenta: transcriptional, epigenetic, and physiological integration during development
    Emin Maltepe
    Department of Pediatrics, Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, University of California, San Francisco, 513 Parnassus Ave HSE 1401, Box 1346, San Francisco, CA 94143 1346, USA
    J Clin Invest 120:1016-25. 2010
    ..Developing an understanding of these pathways contributes to an improvement of our models for studying trophoblast biology and sheds light on the etiology of pregnancy complications and the in utero programming of adult diseases...
  49. pmc The bone marrow-derived human mesenchymal stem cell: potential progenitor of the endometrial stromal fibroblast
    Lusine Aghajanova
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, 505 Parnassus Ave, San Francisco, CA 94143 0132, USA
    Biol Reprod 82:1076-87. 2010
    ..In addition, we identified MSC-specific markers that distinguish them from other fibroblasts and, in particular, from hESF, which is of biologic relevance and practical value to the field of endometrial stem cell research...
  50. pmc MicroRNA function is globally suppressed in mouse oocytes and early embryos
    Nayoung Suh
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA
    Curr Biol 20:271-7. 2010
    ..These findings show that miRNA function is globally suppressed during oocyte maturation and preimplantation development and that endo-siRNAs, rather than miRNAs, underlie the Dicer knockout phenotype in oocytes...
  51. ncbi Altered gene expression profiling in endometrium: evidence for progesterone resistance
    Lusine Aghajanova
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143 0132, USA
    Semin Reprod Med 28:51-8. 2010
    ....
  52. pmc The protein kinase A pathway-regulated transcriptome of endometrial stromal fibroblasts reveals compromised differentiation and persistent proliferative potential in endometriosis
    Lusine Aghajanova
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, 505 Parnassus Avenue, San Francisco, California 94143 0132, USA
    Endocrinology 151:1341-55. 2010
    ....
  53. pmc Intact fetal ovarian cord formation promotes mouse oocyte survival and development
    Cory R Nicholas
    Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University, Palo Alto, CA, USA
    BMC Dev Biol 10:2. 2010
    ..Although germline cysts and testicular cords are required for spermatogenesis, the role of cyst and ovarian cord formation in mammalian oocyte development and female fertility has not been determined...
  54. pmc Gene expression during successful implantation in a natural cycle
    Inge Van Vaerenbergh
    Department of Pathology, UZ Brussel and Vrije Universiteit Brussel, Jette, Belgium
    Fertil Steril 93:268.e15-8. 2010
    ..To determine the human endometrial transcriptome during embryonic implantation...
  55. pmc Uterine receptivity to human embryonic implantation: histology, biomarkers, and transcriptomics
    L Aghajanova
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143 0132, USA
    Semin Cell Dev Biol 19:204-11. 2008
    ....

Research Grants30

  1. Center for the Study of Reproductive Biology and Women's Health
    Jeffrey W Pollard; Fiscal Year: 2013
    ..He holds several senior administrative appointments in the College of Medicine and is well able to administer the proposed SCCPIR internally and to enable effective interactions with other SCCPIRs. ..