CANCER CENTER SUPPORT GRANT

Summary

Principal Investigator: TONY R HUNTER
Abstract: The Salk Institute Cancer Center seeks to understand fundamental aspects of biology related to cancer, with the ultimate goal of reducing cancer incidence, morbidity and mortality. The Center includes 30 faculty members organized into three programs doing basic research in the areas of metabolism and cancer, mouse models and cancer stem cells, and growth control and genomic stability. This Cancer Center Support Grant (CCSG) renewal application requests support for professional personnel, planning and evaluation, development, administration, and seven shared resources, including bioinformatics, cytometry and imaging, functional genomics, peptide synthesis, proteomics, shared equipment, and transgenic facilities, in support of the competitively funded, peer-reviewed research projects in the Center.
Funding Period: 1996-12-31 - 2013-11-30
more information: NIH RePORT

Top Publications

  1. pmc Mechanisms of neovascularization and resistance to anti-angiogenic therapies in glioblastoma multiforme
    Yasushi Soda
    Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Med (Berl) 91:439-48. 2013
  2. pmc The role of Nup98 in transcription regulation in healthy and diseased cells
    Tobias M Franks
    Salk Institute for Biological Studies, Molecular and Cellular Biology Laboratory, La Jolla, CA, USA
    Trends Cell Biol 23:112-7. 2013
  3. pmc Identification of a 3-aminoimidazo[1,2-a]pyridine inhibitor of HIV-1 reverse transcriptase
    Daniel Elleder
    The Salk Institute for Biological Studies, Nomis Center for Immunobiology and Microbial Pathogenesis, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Virol J 9:305. 2012
  4. pmc Insights into negative regulation by the glucocorticoid receptor from genome-wide profiling of inflammatory cistromes
    N Henriette Uhlenhaut
    Gene Expression Laboratory, Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Mol Cell 49:158-71. 2013
  5. pmc Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease
    Pei Chi Wei
    Genomics Research Center, Academia Sinica, Taipei, 115, Taiwan
    Mol Cell 48:747-59. 2012
  6. pmc The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination
    Chi Sheng Lu
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 287:43984-94. 2012
  7. pmc Dedifferentiation of neurons and astrocytes by oncogenes can induce gliomas in mice
    Dinorah Friedmann-Morvinski
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Science 338:1080-4. 2012
  8. pmc Poly-small ubiquitin-like modifier (PolySUMO)-binding proteins identified through a string search
    Huaiyu Sun
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Biol Chem 287:42071-83. 2012
  9. pmc A structural basis for the assembly and functions of a viral polymer that inactivates multiple tumor suppressors
    Horng D Ou
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 151:304-19. 2012
  10. pmc Metabolic reprogramming by class I and II histone deacetylases
    Maria M Mihaylova
    Howard Hughes Medical Institute, Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Trends Endocrinol Metab 24:48-57. 2013

Detail Information

Publications182 found, 100 shown here

  1. pmc Mechanisms of neovascularization and resistance to anti-angiogenic therapies in glioblastoma multiforme
    Yasushi Soda
    Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Med (Berl) 91:439-48. 2013
    ..Therefore, these mechanisms should be targeted in addition to anti-angiogenic therapies to achieve better results for patients with GBM...
  2. pmc The role of Nup98 in transcription regulation in healthy and diseased cells
    Tobias M Franks
    Salk Institute for Biological Studies, Molecular and Cellular Biology Laboratory, La Jolla, CA, USA
    Trends Cell Biol 23:112-7. 2013
    ..In addition, we discuss the possibility that dominant negative Nup98 fusion proteins disrupt the transcriptional activity of WT Nup98 in the nucleoplasm to drive AML...
  3. pmc Identification of a 3-aminoimidazo[1,2-a]pyridine inhibitor of HIV-1 reverse transcriptase
    Daniel Elleder
    The Salk Institute for Biological Studies, Nomis Center for Immunobiology and Microbial Pathogenesis, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Virol J 9:305. 2012
    ....
  4. pmc Insights into negative regulation by the glucocorticoid receptor from genome-wide profiling of inflammatory cistromes
    N Henriette Uhlenhaut
    Gene Expression Laboratory, Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Mol Cell 49:158-71. 2013
    ..Furthermore, transcriptional outcome is not established by sequence but is influenced by epigenetic regulators, context, and other unrecognized regulatory determinants...
  5. pmc Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease
    Pei Chi Wei
    Genomics Research Center, Academia Sinica, Taipei, 115, Taiwan
    Mol Cell 48:747-59. 2012
    ..These results suggest that NPGPx is essential for releasing excessive ER stress by enhancing GRP78 chaperone activity to maintain physiological homeostasis...
  6. pmc The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination
    Chi Sheng Lu
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 287:43984-94. 2012
    ..Taken together, these studies suggest that Nbs1 is one important target of RNF8 to regulate DNA DSB repair...
  7. pmc Dedifferentiation of neurons and astrocytes by oncogenes can induce gliomas in mice
    Dinorah Friedmann-Morvinski
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Science 338:1080-4. 2012
    ....
  8. pmc Poly-small ubiquitin-like modifier (PolySUMO)-binding proteins identified through a string search
    Huaiyu Sun
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Biol Chem 287:42071-83. 2012
    ..We suggest that the clustered SIMs in these proteins form distinct SUMO binding domains to recognize diverse forms of protein sumoylation...
  9. pmc A structural basis for the assembly and functions of a viral polymer that inactivates multiple tumor suppressors
    Horng D Ou
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 151:304-19. 2012
    ..E4-ORF3 assembly creates avidity-driven interactions with PML and an emergent MRN binding interface. This reveals an elegant structural solution whereby a small protein forms a multivalent matrix that traps disparate tumor suppressors...
  10. pmc Metabolic reprogramming by class I and II histone deacetylases
    Maria M Mihaylova
    Howard Hughes Medical Institute, Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Trends Endocrinol Metab 24:48-57. 2013
    ..Here, we review the current understanding of how class I and class II HDACs contribute to metabolic control...
  11. pmc The autoregulated instability of Polo-like kinase 4 limits centrosome duplication to once per cell cycle
    Andrew J Holland
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 26:2684-9. 2012
    ..Plk4 autoregulation thus guards against genome instability by limiting centrosome duplication to once per cell cycle...
  12. pmc Protein homeostasis: live long, won't prosper
    Brandon H Toyama
    Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 14:55-61. 2013
    ..This diverse group of long-lived proteins might be particularly prone to accumulation of damage and thus have a crucial role in the functional deterioration of key regulatory processes during ageing...
  13. pmc A peroxisome proliferator-activated receptor-δ agonist provides neuroprotection in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease
    H L Martin
    Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom
    Neuroscience 240:191-203. 2013
    ..These results indicate that agonism of PPARδ provides protection against MPTP toxicity, in agreement with the effects of other PPAR agonists...
  14. pmc Dynamic association of NUP98 with the human genome
    Yun Liang
    Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, La Jolla, California, USA
    PLoS Genet 9:e1003308. 2013
    ..This work demonstrates for the first time that NUP98 dynamically associates with the human genome during differentiation, revealing a role of a nuclear pore protein in regulating developmental gene expression programs...
  15. pmc LKB1 inactivation dictates therapeutic response of non-small cell lung cancer to the metabolism drug phenformin
    David B Shackelford
    Molecular and Cell Biology Laboratory, Dulbecco Center for Cancer Research, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cancer Cell 23:143-58. 2013
    ..This study suggests phenformin as a cancer metabolism-based therapeutic to selectively target LKB1-deficient tumors...
  16. pmc 5' C-rich telomeric overhangs are an outcome of rapid telomere truncation events
    Liana Oganesian
    The Salk Institute for Biological Studies, Molecular and Cellular Biology Department, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    DNA Repair (Amst) 12:238-45. 2013
    ..Rather chromosome ends bearing 5' C-overhangs are a by-product of rapid cleavage events, processing of which occurs independently of the DNA damage response and is partly mediated through the XRCC3 endonuclease...
  17. pmc DNA damage associated with mitosis and cytokinesis failure
    M T Hayashi
    Molecular and Cellular Biology Department, The Salk Institute for Biological Studies, La Jolla, CA, USA
    Oncogene 32:4593-601. 2013
    ..Here, we analyze several distinct malfunctions during mitosis and cytokinesis that lead to de novo DNA damage generation. ..
  18. pmc Dissecting protein function: an efficient protocol for identifying separation-of-function mutations that encode structurally stable proteins
    Johnathan W Lubin
    Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
    Genetics 193:715-25. 2013
    ....
  19. pmc MDM2, MDMX and p53 in oncogenesis and cancer therapy
    Mark Wade
    Center for Genomic Science of Fondazione Istituto Italiano di Tecnologia, Via Adamello 16, 20139 Milan, Italy
    Nat Rev Cancer 13:83-96. 2013
    ..This Review highlights the progress made and pitfalls encountered as the field continues to search for MDM-targeted antitumour agents...
  20. pmc Human telomeres are tethered to the nuclear envelope during postmitotic nuclear assembly
    Laure Crabbe
    Molecular and Cellular Biology Department, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell Rep 2:1521-9. 2012
    ..Our results shed light on the dynamic relocalization of human telomeres during the cell cycle and suggest redundant pathways for tethering telomeres to the nuclear envelope...
  21. pmc Genetic dissection of TAM receptor-ligand interaction in retinal pigment epithelial cell phagocytosis
    Tal Burstyn-Cohen
    The Institute of Dental Sciences, Hebrew University Hadassah, Jerusalem 91120, Israel
    Neuron 76:1123-32. 2012
    ..These results demonstrate that Protein S and Gas6 function as independent, bona fide Mer ligands, and are, to a first approximation, interchangeable with respect to Mer-driven phagocytosis in the retina...
  22. ncbi Super-resolution microscopy: a comparative treatment
    James M Kasuboski
    Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, La Jolla, CA, USA
    Curr Protoc Cytom . 2012
    ..Furthermore, we discuss their various applications in the imaging of both fixed and live biological samples...
  23. pmc Identification of a specific reprogramming-associated epigenetic signature in human induced pluripotent stem cells
    Sergio Ruiz
    Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 109:16196-201. 2012
    ....
  24. pmc The great unravelling: chromatin as a modulator of the aging process
    Roderick J O'Sullivan
    The Salk Institute for Biological Studies, Molecular and Cellular Biology Department, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Biochem Sci 37:466-76. 2012
    ..This review explores the current state of our understanding of the links between chromatin organization and aging...
  25. ncbi DNA hypermethylation in somatic cells correlates with higher reprogramming efficiency
    Maria J Barrero
    Center for Regenerative Medicine in Barcelona, Barcelona, Catalonia, Spain
    Stem Cells 30:1696-702. 2012
    ..We propose that higher reprogramming efficiency correlates with the hypermethylation of tissue-specific genes rather than with a more permissive pluripotency gene network...
  26. pmc Evolution of the chalcone-isomerase fold from fatty-acid binding to stereospecific catalysis
    Micheline N Ngaki
    Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa 50011, USA
    Nature 485:530-3. 2012
    ..Notably, the FAP discovery defines the adaptive evolution of a stereospecific and catalytically 'perfected' enzyme from a non-enzymatic ancestor over a defined period of plant evolution...
  27. pmc Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2
    Nuria Montserrat
    Center for Regenerative Medicine in Barcelona, Dr Aiguader, 88, 08003 Barcelona, Spain
    J Biol Chem 287:24131-8. 2012
    ..Kidney-derived iPSCs may provide a reliable cellular platform for the development of kidney differentiation protocols allowing drug discovery studies and the study of kidney pathology...
  28. ncbi RNP export by nuclear envelope budding
    Emily M Hatch
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell 149:733-5. 2012
    ..identify an alternate pathway for mRNA export in muscle cells where ribonucleoprotein complexes involved in forming neuromuscular junctions transit the nuclear envelope by fusing with and budding through the nuclear membrane...
  29. pmc The BLM helicase contributes to telomere maintenance through processing of late-replicating intermediate structures
    Colleen Barefield
    Molecular and Cellular Biology Department, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Nucleic Acids Res 40:7358-67. 2012
    ..A subset of UFBs originates from telomeric DNA and their frequency correlates with telomere replication defects. We propose that the BLM complex contributes to telomere maintenance through its activity in resolving LRIs...
  30. pmc Transient nuclear envelope rupturing during interphase in human cancer cells
    Jesse D Vargas
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA
    Nucleus 3:88-100. 2012
    ..Our data uncover the existence of a NE instability that has the potential to change the genomic landscape of cancer cells...
  31. pmc Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells
    Rita Vassena
    Stem Cell Bank, Center for Regenerative Medicine in Barcelona, 08003 Barcelona, Spain
    Hum Mol Genet 21:3366-73. 2012
    ....
  32. pmc Protection from liver fibrosis by a peroxisome proliferator-activated receptor δ agonist
    Keiko Iwaisako
    Department of Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 109:E1369-76. 2012
    ....
  33. pmc A PPARγ-FGF1 axis is required for adaptive adipose remodelling and metabolic homeostasis
    Johan W Jonker
    Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 485:391-4. 2012
    ..The discovery of a phenotype for the FGF1 knockout mouse establishes the PPARγ–FGF1 axis as critical for maintaining metabolic homeostasis and insulin sensitization...
  34. pmc Triclocarban mediates induction of xenobiotic metabolism through activation of the constitutive androstane receptor and the estrogen receptor alpha
    Mei Fei Yueh
    Laboratory of Environmental Toxicology, Department of Pharmacology, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 7:e37705. 2012
    ..Activation of these xenobiotic-sensing receptors amplifies gene expression profiles that might represent a mechanistic base for potential human health effects from exposure to TCC...
  35. pmc Circadian clock protein cryptochrome regulates the expression of proinflammatory cytokines
    Rajesh Narasimamurthy
    Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 109:12662-7. 2012
    ..These results offer a mechanistic framework for understanding the link between circadian rhythm disruption and increased susceptibility to chronic inflammatory diseases...
  36. pmc A three-state model of telomere control over human proliferative boundaries
    Anthony J Cesare
    The Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, 10010 North Torrey Pines Rd, La Jolla, CA 92037, USA
    Curr Opin Cell Biol 24:731-8. 2012
    ..In this model telomeres facilitate a controlled permanent cell cycle arrest with a stable diploid genome during differentiation and may serve as an epigenetic sensor of general stress in DNA metabolism processes...
  37. ncbi Lineage conversion methodologies meet the reprogramming toolbox
    Ignacio Sancho-Martinez
    Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nat Cell Biol 14:892-9. 2012
    ..Here we present these different direct conversion methodologies and discuss their potential as alternatives to using induced pluripotent stem cells and differentiation protocols to generate cell populations of a given fate...
  38. pmc Retrotransposon long interspersed nucleotide element-1 (LINE-1) is activated during salamander limb regeneration
    Wei Zhu
    Molecular and Cell Biology Laboratory and Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Dev Growth Differ 54:673-85. 2012
    ....
  39. ncbi Targeting the pregnane X receptor in liver injury
    Tao Li
    The Salk Institute for Biological Studies, Gene Expression Laboratory, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Expert Opin Ther Targets 16:1075-83. 2012
    ....
  40. pmc Small molecule-mediated TGF-β type II receptor degradation promotes cardiomyogenesis in embryonic stem cells
    Erik Willems
    Muscle Development and Regeneration Program, Sanford Burnham Medical Research Institute, La Jolla, CA 92037, USA
    Cell Stem Cell 11:242-52. 2012
    ..ITD-1 is a highly selective TGF-β inhibitor and reveals an unexpected role for TGF-β signaling in controlling cardiomyocyte differentiation from multipotent cardiovascular precursors...
  41. pmc The transcriptional coactivators p/CIP and SRC-1 control insulin resistance through IRS1 in obesity models
    Zhiyong Wang
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, United States of America
    PLoS ONE 7:e36961. 2012
    ..Therefore, our studies indicate that p/CIP and SRC-1 are potential therapeutic targets not only for obesity but also for diabetes...
  42. pmc Activation of germline-specific genes is required for limb regeneration in the Mexican axolotl
    Wei Zhu
    Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Dev Biol 370:42-51. 2012
    ..Given the requirement for FGF signaling in limb development and regeneration, the results suggest that PL1 and PL2 function to establish a unique germline-like state that is associated with successful regeneration...
  43. ncbi Navigating the epigenetic landscape of pluripotent stem cells
    Mo Li
    Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 13:524-35. 2012
    ....
  44. pmc Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc
    Alessandra Giorgetti
    Center of Regenerative Medicine in Barcelona, 08003 Barcelona, Spain
    Proc Natl Acad Sci U S A 109:12556-61. 2012
    ..This system highlights the potential of CB cells and offers an alternative means to the study of cellular plasticity, possibly in the context of drug screening research and of future cell-replacement therapies...
  45. pmc The Bcl6-SMRT/NCoR cistrome represses inflammation to attenuate atherosclerosis
    Grant D Barish
    Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell Metab 15:554-62. 2012
    ..These results reveal that Bcl6-SMRT/NCoR complexes constrain immune responses and contribute to the prevention of atherosclerosis...
  46. pmc Use of a molecular genetic platform technology to produce human Wnt proteins reveals distinct local and distal signaling abilities
    Jennifer L Green
    The Salk Institute for Biological Studies, La Jolla, California, USA
    PLoS ONE 8:e58395. 2013
    ..These engineered cell lines will reduce the cost of making and purifying Wnt proteins and serve as a continuous, reliable and regulatable source of Wnts to research laboratories around the world...
  47. pmc Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics
    Abigail L Buchwalter
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037
    Mol Biol Cell 25:2472-84. 2014
    ..Taken together, our results suggest a transport-independent role for Nup50 in chromatin biology that occurs away from the NPC. ..
  48. pmc Role of activin-A and myostatin and their signaling pathway in human myometrial and leiomyoma cell function
    Md Soriful Islam
    Department of Experimental and Clinical Medicine M S I, O P, M J, M C, P C, Faculty of Medicine, Polytechnic University of Marche, 60126 Ancona, Italy Biotechnology and Microbiology Laboratory M S I, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh Department of Obstetrics and Gynecology W H C, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 Clayton Foundation Laboratories for Peptide Biology P C G, The Salk Institute for Biological Studies, La Jolla, California 92037 Departments of Clinical Science S R G, A C, A L T and Information Engineering P C, Polytechnic University of Marche, 60131 Ancona, Italy Obstetrics and Gynecology Unit P L, Profili Hospital, Fabriano, 60044 Italy Department of Molecular and Developmental Medicine, Obstetrics, and Gynecology F P, University of Siena, 53100 Siena, Italy
    J Clin Endocrinol Metab 99:E775-85. 2014
    ..Uterine leiomyomas are highly prevalent benign tumors of premenopausal women and the most common indication for hysterectomy. However, the exact etiology of this tumor is not fully understood...
  49. pmc Nuclear pores set the speed limit for mitosis
    Abigail Buchwalter
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, 92037 CA, USA
    Cell 156:868-9. 2014
    ..Rodriguez-Bravo et al. report that the nuclear pore complex scaffolds spindle assembly checkpoint signaling in interphase, providing a store of inhibitory signals that limits the speed of the subsequent mitosis. ..
  50. pmc Dedifferentiation and reprogramming: origins of cancer stem cells
    Dinorah Friedmann-Morvinski
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA, USA
    EMBO Rep 15:244-53. 2014
    ..In this review we discuss the similarities between tumor dedifferentiation and somatic cell reprogramming and how this may pose a risk to the application of this new technology in regenerative medicine. ..
  51. pmc Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells
    Aaron Aslanian
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA Electronic address
    DNA Repair (Amst) 15:29-38. 2014
    ..Using these two mass spectrometry-based approaches, we have identified a novel set of MMS-responsive proteins that will expand our understanding of DNA damage signaling. ..
  52. ncbi Proximity-enabled protein crosslinking through genetically encoding haloalkane unnatural amino acids
    Zheng Xiang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037 USA http wang salk edu
    Angew Chem Int Ed Engl 53:2190-3. 2014
    ....
  53. pmc Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1
    Roderick J O'Sullivan
    Molecular and Cellular Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Struct Mol Biol 21:167-74. 2014
    ..The induction of ALT phenotypes as a consequence of ASF1 depletion strongly supports the hypothesis that ALT is a consequence of histone management dysfunction. ..
  54. pmc Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution
    Matthew S Joens
    Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Sci Rep 3:3514. 2013
    ..We demonstrate the advantages of HIM for imaging biological surfaces as well as compare and contrast the effects of sample preparation techniques and their consequences on sub-nanometer ultrastructure. ..
  55. pmc Designer nodal/BMP2 chimeras mimic nodal signaling, promote chondrogenesis, and reveal a BMP2-like structure
    Luis Esquivies
    From the Structural Biology Laboratory
    J Biol Chem 289:1788-97. 2014
    ..Interestingly, the crystal structure of NB250 shows a backbone scaffold similar to that of BMP2. Our results show that these chimeric ligands may have therapeutic implications in cartilage injuries. ..
  56. pmc Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCR
    Irene Coin
    Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell 155:1258-69. 2013
    ..Structural features of the receptor-ligand complex yield molecular insights on the mechanism of receptor activation and the basis for discrimination between agonist and antagonist function...
  57. pmc Genetically encoding photoswitchable click amino acids in Escherichia coli and mammalian cells
    Christian Hoppmann
    Chemical Biology and Proteomics Laboratory, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037 USA http wang salk edu
    Angew Chem Int Ed Engl 53:3932-6. 2014
    ..These genetically encodable PSCaas will prove valuable for engineering photoswitchable bridges into proteins for reversible optogenetic regulation. ..
  58. pmc Nuclear receptors and metabolism: from feast to famine
    Suk Hyun Hong
    Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, 92037, USA
    Diabetologia 57:860-7. 2014
    ..This review focuses on the roles of NHRs in critical metabolic organs, including adipose tissue, liver and muscle, during the fed and fasted states, as well as their roles in circadian metabolism and downstream regulation of FGFs. ..
  59. pmc Multiple Arkadia/RNF111 structures coordinate its Polycomb body association and transcriptional control
    Huaiyu Sun
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA
    Mol Cell Biol 34:2981-95. 2014
    ....
  60. ncbi A genomics approach identifies senescence-specific gene expression regulation
    Daniel H Lackner
    Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA
    Aging Cell 13:946-50. 2014
    ..The majority of gene expression regulation in replicative senescence was shown to occur due to telomere shortening, as exogenous telomerase activity reverted most of these changes. ..
  61. ncbi A Krüppel-like factor downstream of the E3 ligase WWP-1 mediates dietary-restriction-induced longevity in Caenorhabditis elegans
    Andrea C Carrano
    1 Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA 2
    Nat Commun 5:3772. 2014
    ..Our data support a model in which modulation of KLF-1 by WWP-1 regulates diet-restriction-induced longevity. ..
  62. pmc Breaching the nuclear envelope in development and disease
    Emily Hatch
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037
    J Cell Biol 205:133-41. 2014
    ....
  63. pmc Stepwise recruitment of transcellular and paracellular pathways underlies blood-brain barrier breakdown in stroke
    Daniel Knowland
    Department of Developmental and Cell Biology, University of California, Irvine, CA 92697, USA
    Neuron 82:603-17. 2014
    ..Therefore, stepwise impairment of transcellular followed by paracellular barrier mechanisms accounts for the BBB deficits in stroke...
  64. ncbi Nuclear receptors rock around the clock
    Xuan Zhao
    Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA
    EMBO Rep 15:518-28. 2014
    ..As proven pharmacological targets, nuclear receptors are promising targets for therapeutic control of many pathological conditions associated with the disruption of circadian rhythm. ..
  65. pmc Generation of multiciliated cells in functional airway epithelia from human induced pluripotent stem cells
    Amy L Firth
    Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, CA 92037
    Proc Natl Acad Sci U S A 111:E1723-30. 2014
    ..Our report demonstrating the generation of mature multiciliated cells in respiratory epithelium from iPSCs is a significant advance toward modeling a number of human respiratory diseases in vitro. ..
  66. pmc Metabolism goes viral
    Shigeki J Miyake-Stoner
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037 1099, USA
    Cell Metab 19:549-50. 2014
    ..In this issue, Thai et al. (2014) show that adenovirus E4ORF1 activates MYC glycolytic targets to induce a Warburg-like effect that converts glucose into nucleotides for viral replication. ..
  67. pmc Role of BRCA1 in brain development
    Gerald M Pao
    Laboratory of Genetics and Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037
    Proc Natl Acad Sci U S A 111:E1240-8. 2014
    ....
  68. pmc Road to exercise mimetics: targeting nuclear receptors in skeletal muscle
    Weiwei Fan
    Gene Expression Laboratory Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Mol Endocrinol 51:T87-T100. 2013
    ....
  69. pmc Psy2 targets the PP4 family phosphatase Pph3 to dephosphorylate Mth1 and repress glucose transporter gene expression
    Hui Ma
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA
    Mol Cell Biol 34:452-63. 2014
    ..We conclude that the Pph3-Psy2 phosphatase modulates Mth1 activity to facilitate precise regulation of HXT gene expression by glucose. ..
  70. pmc Rev-Erbs repress macrophage gene expression by inhibiting enhancer-directed transcription
    Michael T Y Lam
    Department of Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nature 498:511-5. 2013
    ..These studies provide evidence for a direct role of eRNAs in contributing to enhancer functions and suggest that Rev-Erbs act to suppress gene expression at a distance by repressing eRNA transcription...
  71. pmc Coordinate transcriptional and translational repression of p53 by TGF-β1 impairs the stress response
    Fernando J López-Díaz
    Regulatory Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 50:552-64. 2013
    ..Thus, crosstalk between the TGF-β and p53 pathways defines a major node of regulation in the cellular stress response, enhancing drug resistance...
  72. pmc Local circadian clock gates cell cycle progression of transient amplifying cells during regenerative hair cycling
    Maksim V Plikus
    Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Proc Natl Acad Sci U S A 110:E2106-15. 2013
    ..Our results uncover diurnal mitotic gating as the essential protective mechanism in highly proliferative hair follicles and offer strategies for minimizing or maximizing cytotoxicity of radiation therapies...
  73. pmc Multiple genetic pathways regulate replicative senescence in telomerase-deficient yeast
    Bari J Ballew
    Salk Institute for Biological Studies, La Jolla, CA 92037 1099, USA
    Aging Cell 12:719-27. 2013
    ....
  74. pmc Parkin mitochondrial translocation is achieved through a novel catalytic activity coupled mechanism
    Xinde Zheng
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Res 23:886-97. 2013
    ..We propose that Parkin translocation is achieved through a novel catalytic activity coupled mechanism. ..
  75. pmc Evidence for orphan nuclear receptor TR4 in the etiology of Cushing disease
    Li Du
    Department of Medicine and Neurosurgery, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 110:8555-60. 2013
    ..Our findings directly link TR4 to the etiology of corticotroph tumors, hormone secretion, and cell growth as well as identify it as a potential target in the treatment of CD...
  76. pmc Stem cells and the developing mammary gland
    Maisam Makarem
    Terry Fox Laboratory, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada
    J Mammary Gland Biol Neoplasia 18:209-19. 2013
    ..Such observations reinforce growing evidence of important differences between tissue-specific fetal and adult cells with stem cell properties and emphasize the merits of investigating their molecular basis. ..
  77. pmc A vitamin D receptor/SMAD genomic circuit gates hepatic fibrotic response
    Ning Ding
    Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell 153:601-13. 2013
    ..Furthermore, the findings suggest VDR ligands as a potential therapy for liver fibrosis...
  78. pmc Paracrine Wnt signaling both promotes and inhibits human breast tumor growth
    Jennifer L Green
    Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 110:6991-6. 2013
    ..Thus, our work suggests that paracrine Wnt signaling can either fuel or repress the growth of human breast cancers depending on yet to be determined aspects of the molecular pathways they express...
  79. pmc Catastrophic nuclear envelope collapse in cancer cell micronuclei
    Emily M Hatch
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 154:47-60. 2013
    ..Our study shows that NE collapse is a key event underlying MN dysfunction and establishes a link between aberrant NE organization and aneuploidy...
  80. pmc The telomere deprotection response is functionally distinct from the genomic DNA damage response
    Anthony J Cesare
    Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 51:141-55. 2013
    ..Telomere deprotection is therefore an epigenetic signal passed between cell generations to ensure that replication-associated telomere-dependent growth arrest occurs in stable diploid G1 phase cells before genome instability can occur. ..
  81. pmc In vivo expression of a light-activatable potassium channel using unnatural amino acids
    Ji Yong Kang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Neuron 80:358-70. 2013
    ..These principles could be generally expanded to other proteins expressed in the brain to enable optical regulation...
  82. pmc Identification of long-lived proteins reveals exceptional stability of essential cellular structures
    Brandon H Toyama
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell 154:971-82. 2013
    ..Our identification of a long-lived proteome reveals cellular components that are at increased risk for damage accumulation, linking long-term protein persistence to the cellular aging process. PAPERCLIP: ..
  83. pmc The paired-box homeodomain transcription factor Pax6 binds to the upstream region of the TRAP gene promoter and suppresses receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation
    Masakazu Kogawa
    From the Departments ofMolecular Biology
    J Biol Chem 288:31299-312. 2013
    ....
  84. pmc Proapoptotic peptide-mediated cancer therapy targeted to cell surface p32
    Lilach Agemy
    1 Cancer Research Center, Sanford Burnham Medical Research Institute, La Jolla, California, USA 2 Center for Nanomedicine and Department of Cell, Molecular and Developmental Biology, University of California, Santa Barbara, California, USA
    Mol Ther 21:2195-204. 2013
    ..The specificity of cell surface p32 for tumor-associated cells, its ability to carry payloads to mitochondria, and the efficacy of the system in important types of cancer make the nanosystem a promising candidate for further development...
  85. pmc Enveloped viruses disable innate immune responses in dendritic cells by direct activation of TAM receptors
    Suchita Bhattacharyya
    Nomis Center for Immunobiology and Microbial Pathogenesis, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell Host Microbe 14:136-47. 2013
    ..Correspondingly, a TAM kinase inhibitor antagonizes the infection of wild-type DCs. Thus, TAM receptors are engaged by viruses in order to attenuate type I IFN signaling and represent potential therapeutic targets. ..
  86. pmc P45 forms a complex with FADD and promotes neuronal cell survival following spinal cord injury
    Tsung Chang Sung
    Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, La Jolla, California, United States of America
    PLoS ONE 8:e69286. 2013
    ..Understanding p45-mediated cellular and molecular mechanisms may provide insights into facilitating nerve regeneration in humans. ..
  87. pmc Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity
    Zheng Xiang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Methods 10:885-8. 2013
    ..We demonstrate the utility of this bond for enabling irreversible binding between an affibody and its protein substrate, capturing peptide-protein interactions in mammalian cells, and improving the photon output of fluorescent proteins. ..
  88. pmc Bbof1 is required to maintain cilia orientation
    Yuan Hung Chien
    The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Development 140:3468-77. 2013
    ..Together, these results suggest that bbof1 is a basal body component required in MCCs to align and maintain cilia orientation in response to flow. ..
  89. pmc Identification of three novel natural product compounds that activate PXR and CAR and inhibit inflammation
    Suticha Kittayaruksakul
    Department of Physiology Faculty of Science, Mahidol University, Bangkok, 10400, Thailand
    Pharm Res 30:2199-208. 2013
    ....
  90. pmc Imaging spinal neuron ensembles active during locomotion with genetically encoded calcium indicators
    Christopher A Hinckley
    Howard Hughes Medical Institute and Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Ann N Y Acad Sci 1279:71-9. 2013
    ....
  91. pmc A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells
    Michael J Edel
    Center of Regenerative Medicine in Barcelona, Dr, Aiguader 88, 08003 Barcelona, Spain
    Cell Commun Signal 9:8. 2011
    ..Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field...
  92. pmc Lifespan extension induced by AMPK and calcineurin is mediated by CRTC-1 and CREB
    William Mair
    The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 470:404-8. 2011
    ....
  93. pmc Regenerating the epigenome
    Maria J Barrero
    Center of Regenerative Medicine in Barcelona, Dr Aiguader 88, 08003 Barcelona, Spain
    EMBO Rep 12:208-15. 2011
    ..Understanding this epigenetic plasticity will allow the development of strategies to stimulate the regeneration of damaged tissues and organs in humans...
  94. pmc Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system
    Nuria Montserrat
    Center of Regenerative Medicine of Barcelona, Doctor Aiguader 88, P7, 08003 Barcelona, Spain
    J Biol Chem 286:12417-28. 2011
    ..After a protocol of serial transfections using poly-β-amino esters, we report a simple methodology to generate human iPS cells from human fibroblasts avoiding the use of viral vectors...
  95. pmc Transdifferentiation of glioblastoma cells into vascular endothelial cells
    Yasushi Soda
    Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:4274-80. 2011
    ..We suggest that the TDEC is an important player in the resistance to anti-VEGF therapy, and hence a potential target for GBM therapy...
  96. ncbi Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration
    Chris Jopling
    Center of Regenerative Medicine in Barcelona, Dr Aiguader, 88, 08003 Barcelona, Spain
    Nat Rev Mol Cell Biol 12:79-89. 2011
    ..Current research aims to understand how these processes work and to eventually harness them for use in regenerative medicine...
  97. pmc Cre-LoxP-regulated expression of monoclonal antibodies driven by an ovalbumin promoter in primary oviduct cells
    Isao Oishi
    Health Research Institute, National Institute of Advanced Industrial Science and Technology, 1 8 31, Midorioka, Ikeda, Osaka 563 8577, Japan
    BMC Biotechnol 11:5. 2011
    ..Ovalbumin promoters can be used as oviduct-specific regulatory sequences in transgenic chickens, but their promoter activities are not high, according to previous reports...
  98. pmc Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase connects energy sensing to mitophagy
    Daniel F Egan
    Molecular and Cell Biology Laboratory, Dulbecco Center for Cancer Research, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Science 331:456-61. 2011
    ..These findings uncover a conserved biochemical mechanism coupling nutrient status with autophagy and cell survival...
  99. pmc Esrrg functions in early branch generation of the ureteric bud and is essential for normal development of the renal papilla
    Rachel Berry
    MRC Human Genetics Unit, Institute of Genetic and Molecular Medicine, Western General Hospital, Edinburgh EH4 2XU, UK
    Hum Mol Genet 20:917-26. 2011
    ..Taken together, these results suggest that Esrrg is required for early branching events of the ureteric duct that occur prior to the onset of nephrogenesis. These findings confirm ESRRG as a strong candidate gene for CAKUT...
  100. pmc Protein kinase signaling networks in cancer
    John Brognard
    Signalling Networks in Cancer Group, Cancer Research UK, Paterson Institute for Cancer Research, The University of Manchester, Manchester, UK
    Curr Opin Genet Dev 21:4-11. 2011
    ..This review will highlight recent insights into known pathways essential for tumorigenesis and discuss exciting new pathways for therapeutic intervention...