Pck1

Summary

Gene Symbol: Pck1
Description: phosphoenolpyruvate carboxykinase 1
Alias: GTP, PCK, PEPCK-C, Pepck, RATPEPCK, phosphoenolpyruvate carboxykinase, cytosolic [GTP], phosphoenolpyruvate carboxykinase (GTP), phosphoenolpyruvate carboxykinase 1 (soluble), phosphoenolpyruvate carboxykinase 1, cytosolic, phosphoenolpyruvate carboxykinase, cytosolic (GTP), phosphoenolpyruvate carboxylase
Species: rat

Top Publications

  1. ncbi Promoter polymorphism in PCK1 (phosphoenolpyruvate carboxykinase gene) associated with type 2 diabetes mellitus
    Henian Cao
    Vascular Biology Research Group, Robarts Research Institute, and Department of Medicine, University of Western Ontario, London, Ontario, Canada N6A 5K8
    J Clin Endocrinol Metab 89:898-903. 2004
  2. ncbi Glyceroneogenesis comes of age
    Elmus G Beale
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    FASEB J 16:1695-6. 2002
  3. ncbi Overcoming diabetes-induced hyperglycemia through inhibition of hepatic phosphoenolpyruvate carboxykinase (GTP) with RNAi
    Alícia G Gómez-Valadés
    Biophysics Unit, Department of Physiological Sciences II, IDIBELL University of Barcelona, L Hospitalet del Llobregat, Spain
    Mol Ther 13:401-10. 2006
  4. ncbi Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression
    R W Hanson
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4936, USA
    Annu Rev Biochem 66:581-611. 1997
  5. pmc Phosphoenolpyruvate carboxykinase and pyruvate carboxylase in developing rat liver
    F J Ballard
    Biochem J 104:866-71. 1967
  6. ncbi Acute and selective regulation of glyceroneogenesis and cytosolic phosphoenolpyruvate carboxykinase in adipose tissue by thiazolidinediones in type 2 diabetes
    T Cadoudal
    INSERM UMR S 530 747, Paris, France
    Diabetologia 50:666-75. 2007
  7. ncbi Liver lipid molecules induce PEPCK-C gene transcription and attenuate insulin action
    Guoxun Chen
    Department of Nutrition, The University of Tennessee at Knoxville, 229 Jessie Harris Building, 1215 West Cumberland Avenue, Knoxville, TN 37996, USA
    Biochem Biophys Res Commun 361:805-10. 2007
  8. ncbi Inhibition of glucagon-signaling and downstream actions by interleukin 1beta and tumor necrosis factor alpha in cultured primary rat hepatocytes
    B Christ
    Institut fur Biochemie und Molekulare Zellbiologie, Georg August Universitat, Humboldtallee, Gottingen, Germany
    Horm Metab Res 40:18-23. 2008
  9. ncbi Effect of reduced maternal inspired oxygen on hepatic glucose metabolism in the rat fetus
    Prabhu S Parimi
    Robert Schwartz MD Center for Metabolism and Nutrition, Departments of Pediatrics and Biochemistry, Case Western Reserve University School of Medicine, MetroHealth Medical Center, Cleveland, Ohio 44109 1998, USA
    Pediatr Res 53:325-32. 2003
  10. ncbi Impact of interleukin-6 on the glucose metabolic capacity in rat liver
    Bianca Lienenlüke
    First Department of Medicine, Martin Luther University of Halle Wittenberg, Heinrich Damerow Strasse 1, 06120 Halle Saale, Germany
    Histochem Cell Biol 128:371-7. 2007

Scientific Experts

  • Paul W Caton
  • J Takaya
  • Prabhu S Parimi
  • Hiroaki Oda
  • Gillian Hamilton
  • Jiandi Zhang
  • Guoxun Chen
  • Sébastien Banzet
  • H K Berthold
  • Carmen E Perrone
  • B Christ
  • Yanmei Dong
  • P Dunten
  • Elmus G Beale
  • Kazuya Yamada
  • L Wegner
  • Colleen M Novak
  • Yan Zhang
  • Sarah M Sullivan
  • Alícia G Gómez-Valadés
  • Claude Forest
  • J Wu
  • Anne Marie Jaubert
  • Mahua Choudhury
  • Rui Li
  • Yang Li
  • Wei Chen
  • Chang Wen Hsieh
  • Rita S Strakovsky
  • Sarawut Jitrapakdee
  • Ming Juan Luo
  • Thomas Cadoudal
  • Yusuke Inoue
  • Todd Holyoak
  • Jordi Bermudez
  • Dalila Azzout-Marniche
  • Bianca Lienenlüke
  • Yong Fen Zhu
  • Jose C Perales
  • Fabienne Rajas
  • T Cadoudal
  • Colleen M Croniger
  • Ramon Bartrons
  • Anna Vidal-Alabró
  • Yanfen Niu
  • I Gouni-Berthold
  • Shih Pei Chang
  • Martine Glorian
  • David Yeagley
  • Sandra M Lelli
  • Haiyan Xu
  • Stephanie T Yerkovich
  • Kaushik Chakravarty
  • Henian Cao
  • Richard W Hanson
  • Lea Reshef
  • L L Ge
  • S S Hou
  • J Xu
  • W Wang
  • J J Yin
  • N Cheng
  • M Yin
  • Eric Duplus
  • Heather L Wilson
  • K D Drewnowsk
  • Nawal Zeitouni
  • Sylvie Durant
  • Graziella Penot
  • Fatoumata Niang
  • S Song
  • Jianqi Yang
  • Shaikh Mizanoor Rahman
  • Dan Zhou
  • Maria Hatzoglou
  • Jacob E Friedman
  • Rachel C Janssen
  • Michelle Beidelschies
  • Yuebin Ge
  • Yuan Xiang Pan
  • Jill Schroeder-Gloeckler
  • Ishtiaq Qadri
  • Michelle Puchowicz
  • Ilya Bederman
  • Xiyuan Zhang
  • Charles Huang
  • Tian Shu Zeng
  • Yoshiki Katayama
  • Aurélie Massias
  • Miguel Chillon

Detail Information

Publications70

  1. ncbi Promoter polymorphism in PCK1 (phosphoenolpyruvate carboxykinase gene) associated with type 2 diabetes mellitus
    Henian Cao
    Vascular Biology Research Group, Robarts Research Institute, and Department of Medicine, University of Western Ontario, London, Ontario, Canada N6A 5K8
    J Clin Endocrinol Metab 89:898-903. 2004
    We sequenced the promoter and coding regions of PCK1 encoding cytosolic phosphoenolpyruvate carboxykinase from genomic DNA of subjects with type 2 diabetes mellitus (DM)...
  2. ncbi Glyceroneogenesis comes of age
    Elmus G Beale
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    FASEB J 16:1695-6. 2002
    ..This short review highlights a series of recent discoveries showing that glyceroneogenesis is important in lipid homeostasis...
  3. ncbi Overcoming diabetes-induced hyperglycemia through inhibition of hepatic phosphoenolpyruvate carboxykinase (GTP) with RNAi
    Alícia G Gómez-Valadés
    Biophysics Unit, Department of Physiological Sciences II, IDIBELL University of Barcelona, L Hospitalet del Llobregat, Spain
    Mol Ther 13:401-10. 2006
    Phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.32) is the rate-controlling enzyme in gluconeogenesis...
  4. ncbi Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression
    R W Hanson
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4936, USA
    Annu Rev Biochem 66:581-611. 1997
    Phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) (PEPCK) is a key enzyme in the synthesis of glucose in the liver and kidney and of glyceride-glycerol in white adipose tissue and the small intestine...
  5. pmc Phosphoenolpyruvate carboxykinase and pyruvate carboxylase in developing rat liver
    F J Ballard
    Biochem J 104:866-71. 1967
    ..7. It is suggested that the appearance of the soluble phosphoenolpyruvate carboxykinase at birth initiates the rapid increase in overall gluconeogenesis at this stage...
  6. ncbi Acute and selective regulation of glyceroneogenesis and cytosolic phosphoenolpyruvate carboxykinase in adipose tissue by thiazolidinediones in type 2 diabetes
    T Cadoudal
    INSERM UMR S 530 747, Paris, France
    Diabetologia 50:666-75. 2007
    Regulation of glyceroneogenesis and its key enzyme cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C) plays a major role in the control of fatty acid release from adipose tissue...
  7. ncbi Liver lipid molecules induce PEPCK-C gene transcription and attenuate insulin action
    Guoxun Chen
    Department of Nutrition, The University of Tennessee at Knoxville, 229 Jessie Harris Building, 1215 West Cumberland Avenue, Knoxville, TN 37996, USA
    Biochem Biophys Res Commun 361:805-10. 2007
    Cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C) plays key roles in gluconeogenesis, glyceroneogenesis, and cataplerosis...
  8. ncbi Inhibition of glucagon-signaling and downstream actions by interleukin 1beta and tumor necrosis factor alpha in cultured primary rat hepatocytes
    B Christ
    Institut fur Biochemie und Molekulare Zellbiologie, Georg August Universitat, Humboldtallee, Gottingen, Germany
    Horm Metab Res 40:18-23. 2008
    In cultured rat hepatocytes, glucagon increased the phosphoenolpyruvate carboxykinase (PCK1) mRNA by increasing cellular cAMP concentrations...
  9. ncbi Effect of reduced maternal inspired oxygen on hepatic glucose metabolism in the rat fetus
    Prabhu S Parimi
    Robert Schwartz MD Center for Metabolism and Nutrition, Departments of Pediatrics and Biochemistry, Case Western Reserve University School of Medicine, MetroHealth Medical Center, Cleveland, Ohio 44109 1998, USA
    Pediatr Res 53:325-32. 2003
    ..hepatic glycogen stores in the fetus and delayed induction of cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C) at birth...
  10. ncbi Impact of interleukin-6 on the glucose metabolic capacity in rat liver
    Bianca Lienenlüke
    First Department of Medicine, Martin Luther University of Halle Wittenberg, Heinrich Damerow Strasse 1, 06120 Halle Saale, Germany
    Histochem Cell Biol 128:371-7. 2007
    ..by monitoring glycogen degradation and the expression of the gluconeogenic phosphoenolpyruvate carboxykinase (PCK1) in rat livers during the daily feeding rhythm. Eight hours after i.p...
  11. ncbi Phosphorylation of carbovir enantiomers by cellular enzymes determines the stereoselectivity of antiviral activity
    W H Miller
    Division of Experimental Therapy, Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709
    J Biol Chem 267:21220-4. 1992
    ..Thus, the contrasting HIV-inhibitory activities of carbovir enantiomers were due to differential phosphorylation by cellular enzymes and not due to enantioselectivity of HIV reverse transcriptase...
  12. doi Fructose induces gluconeogenesis and lipogenesis through a SIRT1-dependent mechanism
    Paul W Caton
    Department of Translational Medicine and Therapeutics, Bart s and The London School of Medicine and Dentistry, William Harvey Research Institute, Queen Mary University of London, London, UK
    J Endocrinol 208:273-83. 2011
    ..in peroxisome proliferator-activated receptor coactivator 1-alpha (PGC1α) and phosphoenolpyruvate carboxykinase (PEPCK; gene code Pck1) gene expression, PEPCK activity, and hepatocyte glucose production...
  13. doi Down-regulation of hepatic phosphoenolpyruvate carboxykinase expression in magnesium-deficient rats
    Junji Takaya
    Department of Pediatrics, Kansai Medical University, Fumizono cho, Moriguchi Shi, Osaka, Japan
    Magnes Res 25:131-9. 2012
    ..to assess mRNA for 11β hydroxysteroid dehydrogenase-1 (11β-HSD1), 11β-HSD2, phosphoenolpyruvate carboxykinase (PEPCK), peroxisome proliferator-activated receptor α (PPARα), and glucocorticoid receptor in the liver...
  14. doi Hepatic phosphoenolpyruvate carboxykinase expression after gastric bypass surgery in rats with type 2 diabetes mellitus
    J Wu
    Zunyi Medical University, Zunyi, China
    Genet Mol Res 14:16938-47. 2015
    The objective of this study was to investigate the mRNA expression of hepatic phosphoenolpyruvate carboxykinase (PEPCK) after gastric bypass surgery (GBS) in rats with type 2 diabetic mellitus (T2DM)...
  15. doi Endotoxin induced hyperlactatemia and hypoglycemia is linked to decreased mitochondrial phosphoenolpyruvate carboxykinase
    Paul W Caton
    William Harvey Research Institute, Bart s and The London, Queen Mary s School of Medicine and Dentistry, London EC1M 6BQ, United Kingdom
    Life Sci 84:738-44. 2009
    Phosphoenolpyruvate carboxykinase (PEPCK) is the rate limiting enzyme for gluconeogenesis, and plays a key role in recycling lactate for glucose production...
  16. doi Genomic and metabolic responses to methionine-restricted and methionine-restricted, cysteine-supplemented diets in Fischer 344 rat inguinal adipose tissue, liver and quadriceps muscle
    Carmen E Perrone
    Orentreich Foundation for the Advancement of Science, Inc, Cold Spring on Hudson, NY, USA
    J Nutrigenet Nutrigenomics 5:132-57. 2012
    ..Genomic and metabolomic studies were conducted to identify potential mechanisms by which MR induces favorable metabolic effects, and that are reversed by cysteine supplementation...
  17. doi Phenobarbital reduces blood glucose and gluconeogenesis through down-regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression in rats
    Hiroaki Oda
    Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences, Nagoya University, Nagoya 464 8601, Japan Electronic address
    Biochem Biophys Res Commun 466:306-11. 2015
    The regulatory mechanism of phosphoenolpyruvate carboykinase (GTP) (EC 4.1.1.32) (PEPCK) gene expression and gluconeogenesis by phenobarbital (PB), which is known to induce drug-metabolizing enzymes, was investigated...
  18. doi Upregulation of hepatic 11β-hydroxysteroid dehydrogenase-1 expression in calcium-deficient rats
    Junji Takaya
    Department of Pediatrics, Kansai Medical University, Moriguchi, Japan
    Ann Nutr Metab 59:73-8. 2011
    ..Many epidemiologic studies have reported a link between calcium (Ca) deficiency and metabolic syndrome. In this study, we examine Ca deficiency in rats and whether changes in glucocorticoid metabolism are induced...
  19. pmc Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release
    Anne Marie Jaubert
    Institut National de la Santé et de la Recherche Médicale UMR S 747, Universite Paris Descartes, Pharmacologie Toxicologie et Signalisation Cellulaire, Paris, France
    PLoS ONE 7:e40650. 2012
    ..The latter relies on the induction of cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), the key enzyme for glyceroneogenesis...
  20. ncbi [Effects of expression of fatty acid synthetase and phosphoenolpyruvate carboxykinase in offspring rats by high protein intake in pregnancy]
    Yanmei Dong
    Department of Public Health, Qiqihar Medical University, Qiqihar 161006, China
    Wei Sheng Yan Jiu 38:287-9. 2009
    To investigate the effect of pregnant nutritional manipulation on offspring mRNA expression of fatty acid synthetase (FAS) and phosphoenolpyruvate carboxykinase (PEPCK).
  21. ncbi Effects of bis(alpha-furancarboxylato)oxovanadium(IV) on glucose metabolism in fat-fed/streptozotocin-diabetic rats
    Yanfen Niu
    Yunnan Pharmacological Laboratories of Natural Products, Kunming Medical College, Kunming, PR China
    Eur J Pharmacol 572:213-9. 2007
    ..These findings suggested that BEOV had anti-diabetic and insulin-sensitizing effects in the diabetic rats, exhibiting the potential to be developed as a new therapeutic agent for the treatment of type 2 diabetes...
  22. ncbi Large-scale study of the -232C > G polymorphism of PCK1 in Type 2 diabetes
    L Wegner
    Steno Diabetes Center, Gentofte, Denmark
    Diabet Med 23:1140-4. 2006
    Phosphoenolpyruvate carboxykinase (PEPCK) is a catalyst of the rate-limiting step in the gluconeogenic pathway and is regulated at the transcriptional level predominantly by insulin, glucocorticoids, glucagon, and cAMP...
  23. ncbi [The effect of calorie restriction on the expression of liver's gluconeogenesis genes of rats fed a high fat diet]
    Ming Juan Luo
    Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
    Zhonghua Gan Zang Bing Za Zhi 16:125-8. 2008
    To observe the effect of calorie restriction on the high fat diet rats mRNA expressions of liver forkhead box O1(FoxO1), phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G-6-P) and to explore the possible mechanisms.
  24. ncbi Efficient delivery of siRNA using dendritic poly(L-lysine) for loss-of-function analysis
    Yusuke Inoue
    Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi ku, Fukuoka 819 0395, Japan
    J Control Release 126:59-66. 2008
    ..In addition, the knockdown of PEPCK, which is the rate-limiting enzyme for gluconeogenesis, led to a reduction in glucose production in rat hepatoma ..
  25. ncbi Suppression of phosphoenolpyruvate carboxykinase gene expression by reduced endogenous glutathione level
    Jiandi Zhang
    Center for Human Nutrition, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Biochim Biophys Acta 1772:1175-81. 2007
    ..to reduced mRNA levels of several key enzymes in energy homeostasis, including phosphoenolpyruvate carboxylkinase (PEPCK), the rate-limiting enzyme in gluconeogenesis...
  26. ncbi [The effect of endotoxemia on gluconeogenesis in livers and kidneys during acute hepatic failure]
    Yong Fen Zhu
    Department of Infectious Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
    Zhonghua Gan Zang Bing Za Zhi 15:672-5. 2007
    ..To explore the effects of endotoxemia on gluconeogenesis in livers and kidneys during acute hepatic failure...
  27. ncbi Structures of rat cytosolic PEPCK: insight into the mechanism of phosphorylation and decarboxylation of oxaloacetic acid
    Sarah M Sullivan
    Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Biochemistry 46:10078-88. 2007
    The structures of the rat cytosolic isoform of phosphoenolpyruvate carboxykinase (PEPCK) reported in the PEPCK-Mn2+, -Mn2+-oxaloacetic acid (OAA), -Mn2+-OAA-Mn2+-guanosine-5'-diphosphate (GDP), and -Mn2+-Mn2+-guanosine-5'-tri-phosphate (..
  28. pmc Pck1 gene silencing in the liver improves glycemia control, insulin sensitivity, and dyslipidemia in db/db mice
    Alícia G Gómez-Valadés
    Biophysics Unit, Department de Ciències Fisiològuiques II, IDIBELL University of Barcelona, Barcelona, Spain
    Diabetes 57:2199-210. 2008
    Cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C; encoded by Pck1) catalyzes the first committed step in gluconeogenesis. Extensive evidence demonstrates a direct correlation between PEPCK-C activity and glycemia control...
  29. ncbi Retinoids upregulate phosphoenolpyruvate carboxykinase and glyceroneogenesis in human and rodent adipocytes
    Thomas Cadoudal
    Institut National de la Recherche Médicale UMR S 747, Universite Paris Descartes, F 75006 Paris, France
    J Nutr 138:1004-9. 2008
    ..Glyceroneogenesis and cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), which is the key enzyme in this pathway, are both regulated by a series of hormones and nutrients, among which ..
  30. pmc Structure, mechanism and regulation of pyruvate carboxylase
    Sarawut Jitrapakdee
    Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
    Biochem J 413:369-87. 2008
    ..The present review summarizes these advances and also offers some prospects in terms of future directions for the study of this important enzyme...
  31. pmc Enzymes with lid-gated active sites must operate by an induced fit mechanism instead of conformational selection
    Sarah M Sullivan
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Proc Natl Acad Sci U S A 105:13829-34. 2008
    ..The structural data are consistent with a model in that the energy input from substrate association results in changes in the free energy landscape for the protein, allowing for structural transitions along an induced fit pathway...
  32. doi A Leu184Val polymorphism in PCK1 gene is associated with type 2 diabetes in Eastern Chinese population with BMI<23 kg/m2
    Yan Dong
    Department of Endocrinology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
    Diabetes Res Clin Pract 83:227-32. 2009
    ..29 [95% CI 1.03-1.64]), especially in subgroup with the BMI<23 kg/m(2) (P=0.018). The enzymatic activity of PEPCK-C containing Leu184 was higher than that of PEPCK-C containing Val184 (P<0.01)...
  33. pmc Endurance capacity, not body size, determines physical activity levels: role of skeletal muscle PEPCK
    Colleen M Novak
    Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota, USA
    PLoS ONE 4:e5869. 2009
    ..identified one potential mechanism for the lean, active phenotype in rats, namely high levels of skeletal muscle PEPCK. Therefore, the lean phenotype is characterized by high endurance capacity and high activity and may stem from ..
  34. doi Control of gluconeogenic genes during intense/prolonged exercise: hormone-independent effect of muscle-derived IL-6 on hepatic tissue and PEPCK mRNA
    Sébastien Banzet
    Department of Human Factors, Military Health Service Research Center, La Tronche, France
    J Appl Physiol (1985) 107:1830-9. 2009
    ..decrease in liver glycogen and an increase in gluconeogenic gene mRNA levels, phosphoenolpyruvate-carboxykinase (PEPCK), glucose-6-phosphatase (G6P), and peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha)..
  35. pmc C/EBPβ is AMP kinase sensitive and up-regulates PEPCK in response to ER stress in hepatoma cells
    Mahua Choudhury
    Department of Pediatrics, University of Colorado Denver, Aurora, CO 80045, USA
    Mol Cell Endocrinol 331:102-8. 2011
    ..ER stress contributed to transcriptional activation of the gluconeogenic phosphoenolpyruvate carboxykinase (PEPCK) promoter in Huh7 and HepG2 cells via cAMP binding motif (CRE site)...
  36. pmc Gestational high fat diet programs hepatic phosphoenolpyruvate carboxykinase gene expression and histone modification in neonatal offspring rats
    Rita S Strakovsky
    Department of Food Science and Human Nutrition, and Division of Nutritional Sciences, University of Illinois at Urbana Champaign, 461 Bevier Hall, MC 182, 905 South Goodwin Avenue, Urbana, IL 61801, USA
    J Physiol 589:2707-17. 2011
    ..In addition to increased transcription rate, a gestational HF diet resulted in modifications of the Pck1 histone code in livers of offspring...
  37. pmc Function of phosphoenolpyruvate carboxykinase in mammary gland epithelial cells
    Chang Wen Hsieh
    Department of Nutrition, Case Western Reserve University, Cleveland, OH 44106, USA
    J Lipid Res 52:1352-62. 2011
    Previously, we have shown that Pck1 expression in mammary gland adipocytes and white adipose tissue maintains triglyceride stores through glyceroneogenesis, and these lipids were used for synthesis of milk triglycerides during lactation...
  38. doi Retinoids induced Pck1 expression and attenuated insulin-mediated suppression of its expression via activation of retinoic acid receptor in primary rat hepatocytes
    Yan Zhang
    Department of Nutrition, University of Tennessee at Knoxville, 229 Jessie Harris Building, 1215 West Cumberland Avenue, Knoxville, TN 37996, USA
    Mol Cell Biochem 355:1-8. 2011
    ..in hepatic glucose and lipid metabolism, such as the cytosolic form of phosphoenolpyruvate carboxykinase gene (Pck1)...
  39. pmc Insulin-regulated Srebp-1c and Pck1 mRNA expression in primary hepatocytes from zucker fatty but not lean rats is affected by feeding conditions
    Yan Zhang
    Department of Nutrition, University of Tennessee at Knoxville, Knoxville, Tennessee, United States of America
    PLoS ONE 6:e21342. 2011
    ..The mRNA levels of one insulin-induced (Srebp-1c) and one insulin-suppressed (Pck1) genes in response to insulin, glucagon, and compactin treatments in hepatocytes from ad libitum ZL and ZF rats ..
  40. ncbi Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site
    Pete Dunten
    Roche Research Center, Hoffmann La Roche Inc, Nutley, NJ 07110, USA
    J Mol Biol 316:257-64. 2002
    We report crystal structures of the human enzyme phosphoenolpyruvate carboxykinase (PEPCK) with and without bound substrates...
  41. pmc The interplay of ubiquitous DNA-binding factors, availability of binding sites in the chromatin, and DNA methylation in the differential regulation of phosphoenolpyruvate carboxykinase gene expression
    S Faber
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232
    Nucleic Acids Res 19:4681-8. 1991
    We have identified DNA elements in the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter which are bound 'in vivo' by proteins under conditions of basal level gene expression and have evaluated several hypothesis to account for the ..
  42. pmc Interaction of a liver-specific factor with an enhancer 4.8 kilobases upstream of the phosphoenolpyruvate carboxykinase gene
    Y T Ip
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
    Mol Cell Biol 10:3770-81. 1990
    ..a series of five DNase-I hypersensitive (HS) sites within and around the rat phosphoenolpyruvate carboxykinase (PEPCK) gene...
  43. ncbi Cysteine 288: an essential hyperreactive thiol of cytosolic phosphoenolpyruvate carboxykinase (GTP)
    C T Lewis
    Department of Biochemistry, College of Medicine, University of Tennessee, Memphis 38163
    J Biol Chem 264:27-33. 1989
    ..0. Purification and sequencing of the labeled peptide identifies the hyperreactive essential cysteine as Cys-288. This cysteine lies between two putative phosphoryl-binding domains and within a hydrophobic sequence...
  44. ncbi Evidence that stimulation of gluconeogenesis by fatty acid is mediated through thermodynamic mechanisms
    M N Berry
    Department of Medical Biochemistry, School of Medicine, Flinders University of South Australia, Bedford Park, Australia
    FEBS Lett 231:19-24. 1988
    ..e. mitochondrial membrane and redox potentials and cellular phosphorylation potential). These findings suggest that the fatty acid stimulation of glucose synthesis is in part mediated through thermodynamic mechanisms...
  45. ncbi Rat hepatic cytosolic phosphoenolpyruvate carboxykinase (GTP). Structures of the protein, messenger RNA, and gene
    E G Beale
    J Biol Chem 260:10748-60. 1985
    ..We conclude that mRNAPEPCK synthesis initiates with an adenine residue 69 base pairs 5' of the BglII site; this corresponds to the 3' most transcription initiation site determined in vitro...
  46. ncbi Characterization of the phosphoenolpyruvate carboxykinase (GTP) promoter-regulatory region. II. Identification of cAMP and glucocorticoid regulatory domains
    J M Short
    J Biol Chem 261:9721-6. 1986
    Hormonal regulatory elements within the phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) (PEPCK) promoter region were mapped using a series of 5' deletions linked to the amino-3'-glycosyl phosphotransferase structural gene...
  47. pmc Gluconeogenesis in developing rat kidney cortex
    A Zorzoli
    Biochem J 111:181-5. 1969
    ..starvation was studied in perinatal animals. The results indicate that the ability to increase the rate of glucose synthesis as a result of starvation is not present at birth, but develops some time after the second postnatal day...
  48. pmc Induction and suppression of the key enzymes of glycolysis and gluconeogenesis in isolated perfused rat liver in response to glucose, fructose and lactate
    J M Wimhurst
    Biochem J 134:143-56. 1973
    ..4.1.1), phosphoenolpyruvate carboxylase (EC 4.1.1.32), fructose 1,6-diphosphatase (EC 3.1.3.11) and glucose 6-phosphatase (EC 3.1.3...
  49. ncbi Identification of a cAMP regulatory region in the gene for rat cytosolic phosphoenolpyruvate carboxykinase (GTP). Use of chimeric genes transfected into hepatoma cells
    A Wynshaw-Boris
    J Biol Chem 259:12161-9. 1984
    ..1.1.32) (PEPCK) in rat liver...
  50. pmc Isolation and characterization of the gene coding for cytosolic phosphoenolpyruvate carboxykinase (GTP) from the rat
    H Yoo-Warren
    Proc Natl Acad Sci U S A 80:3656-60. 1983
    ..The isolated clone, lambda PCK1, contains the complete gene for phosphoenolpyruvate carboxykinase and approximately equal to 7 kilobases (kb) of ..
  51. ncbi Adipose expression of the phosphoenolpyruvate carboxykinase promoter requires peroxisome proliferator-activated receptor gamma and 9-cis-retinoic acid receptor binding to an adipocyte-specific enhancer in vivo
    J H Devine
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Biol Chem 274:13604-12. 1999
    ..has been mapped to approximately 1 kilobase pair upstream of the cytosolic phosphoenolpyruvate carboxykinase (PEPCK) gene...
  52. ncbi Mechanism of fat-induced hepatic gluconeogenesis: effect of metformin
    S Song
    Department of Medicine, University of Melbourne, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia
    Am J Physiol Endocrinol Metab 281:E275-82. 2001
    ..Metformin predominantly inhibits alanine gluconeogenesis by preventing the fat-induced changes in fructose-1,6-bisphosphatase levels...
  53. ncbi Phosphoenolpyruvate carboxykinase is induced in growth-arrested hepatoma cells
    Nawal Zeitouni
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Biochem Biophys Res Commun 290:1513-20. 2002
    Phosphoenolpyruvate carboxykinase (PEPCK) mRNA is elevated in H4IIEC3 rat hepatoma cells cultured at high density, suggesting that PEPCK expression and growth arrest may be coordinately regulated...
  54. ncbi Candidate gene association study of insulin signaling genes and Alzheimer's disease: evidence for SOS2, PCK1, and PPARgamma as susceptibility loci
    Gillian Hamilton
    MRC Centre for Neurodegeneration Research, Department of Neuroscience, Institute of Psychiatry, Kings College London, Denmark Hill, London, UK
    Am J Med Genet B Neuropsychiatr Genet 144:508-16. 2007
    ..In a replication study, we confirmed significant association of SNPs within three genes--PPARgamma, SOS2, and PCK1--with Alzheimer's disease...
  55. ncbi PEPCK mRNA localization in proximal tubule and gene regulation during metabolic acidosis
    K D Drewnowsk
    Department of Medicine, Virginia Commonwealth University, Richmond 23298 0160, USA
    J Physiol Pharmacol 53:3-20. 2002
    To identify the nephron segments expressing PEPCK in control and acidotic conditions, PEPCK mRNA was localized in rat kidney using the technique of reverse transcription and polymerase chain reaction (RT-PCR) in individual microdissected ..
  56. ncbi Evidence for selective induction of phosphoenolpyruvate carboxykinase gene expression by unsaturated and nonmetabolized fatty acids in adipocytes
    Eric Duplus
    INSERM Unit 530, Centre Universitaire des Saints Peres, 45 Rue des Saints Peres, 75006, Paris, France
    J Cell Biochem 85:651-61. 2002
    ..Cytosolic phosphoenolpyruvate carboxykinase (PEPCK) plays a key role in this pathway...
  57. ncbi Different transcription factor binding arrays modulate the cAMP responsivity of the phosphoenolpyruvate carboxykinase gene promoter
    Heather L Wilson
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada
    J Biol Chem 277:43895-902. 2002
    The cAMP responsiveness of the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter is mediated by a cAMP response unit, which includes three CCAAT/enhancer-binding protein (C/EBPs) sites, and a cAMP response element (CRE)...
  58. ncbi Glyceroneogenesis and the triglyceride/fatty acid cycle
    Lea Reshef
    Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Biol Chem 278:30413-6. 2003
  59. ncbi SREBP-1c and Sp1 interact to regulate transcription of the gene for phosphoenolpyruvate carboxykinase (GTP) in the liver
    Kaushik Chakravarty
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4935, USA
    J Biol Chem 279:15385-95. 2004
    ..SREBP-2, inhibit transcription of the gene encoding the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (PEPCK-C). There are two SREBP regulatory elements (SREs) in the PEPCK-C gene promoter (-322 to -313 and -590 to -581)...
  60. ncbi Kupffer cell cytokines interleukin-1beta and interleukin-10 combine to inhibit phosphoenolpyruvate carboxykinase and gluconeogenesis in cultured hepatocytes
    Stephanie T Yerkovich
    Biochemistry and Molecular Biology, University of Western Australia, Nedlands, 35 Stirling Highway, Crawley 6009, WA, Australia
    Int J Biochem Cell Biol 36:1462-72. 2004
    ....
  61. ncbi 3',5'-cyclic adenosine monophosphate response element-binding protein and CCAAT enhancer-binding protein are dispensable for insulin inhibition of phosphoenolpyruvate carboxykinase transcription and for its synergistic induction by protein kinase A and gl
    David Yeagley
    The Pennsylvania State University, College of Medicine, Department of Cellular and Molecular Physiology, C4718, 500 University Drive, Hershey, Pennsylvania 17033, USA
    Mol Endocrinol 19:913-24. 2005
    Phosphoenolpyruvate carboxykinase (PEPCK) transcription is induced by cAMP/protein kinase A (PKA) and glucocorticoids [dexamethasone (Dex)] and is inhibited by insulin to regulate blood glucose...
  62. ncbi SHARP-2/Stra13/DEC1 as a potential repressor of phosphoenolpyruvate carboxykinase gene expression
    Kazuya Yamada
    Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910 1193, Japan
    FEBS Lett 579:1509-14. 2005
    ..protein-2 (SHARP-2) transcriptional repressor on the expression of rat phosphoenolpyruvate carboxykinase (PEPCK) gene was examined...
  63. ncbi Response of glucose metabolism enzymes in an acute porphyria model. Role of reactive oxygen species
    Sandra M Lelli
    Salud Humana y Medio Ambiente DIMXSA, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Ciudad Autonoma de Buenos Aires, Argentina
    Toxicology 216:49-58. 2005
    ..ROS) through assays on hepatic key gluconeogenic and glycogenolytic enzymes; phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP), respectively...
  64. ncbi Dual specificity MAPK phosphatase 3 activates PEPCK gene transcription and increases gluconeogenesis in rat hepatoma cells
    Haiyan Xu
    Millennium Pharmaceuticals Incorporated, Cambridge, Massachussetts 02139, USA
    J Biol Chem 280:36013-8. 2005
    ..In liver, insulin suppresses gluconeogenesis by inhibiting the transcriptions of phosphoenolpyruvate carboxylase (PEPCK) and glucose-6-phosphatase (G6Pase) genes...
  65. ncbi Merit of physical exercise to reverse the higher gene expression of hepatic phosphoenolpyruvate carboxykinase in obese Zucker rats
    Shih Pei Chang
    Department of Physical Education and Department of Medical Technology, Centre Taiwan University of Science and Technology, Taichung City 40601, Taiwan
    Life Sci 79:240-6. 2006
    Effects of endurance training on the phosphoenolpyruvate carboxykinase (PEPCK), a rate-limiting enzyme of gluconeogenesis, were studied in the obese Zucker rats...
  66. ncbi Association of the promoter polymorphism -232C/G of the phosphoenolpyruvate carboxykinase gene (PCK1) with Type 2 diabetes mellitus
    I Gouni-Berthold
    Department of Internal Medicine II, University of Cologne, Cologne, Germany
    Diabet Med 23:419-25. 2006
    The phosphoenolpyruvate carboxykinase gene (PCK1) is a potential candidate gene in the pathogenesis of Type 2 diabetes mellitus...
  67. ncbi Liver glyconeogenesis: a pathway to cope with postprandial amino acid excess in high-protein fed rats?
    Dalila Azzout-Marniche
    INRA, AgroParisTech, UMR914 Nutrition Physiology and Ingestive Behavior, 16 rue Claude Bernard, Paris, F75005, France
    Am J Physiol Regul Integr Comp Physiol 292:R1400-7. 2007
    ..This evidence is based on the concomitant upregulation of phosphoenolpyruvate carboxykinase (PEPCK) gene expression, downregulation of glucose 6-phosphatase catalytic subunit (G6PC1) gene expression, an absence of ..
  68. ncbi Immunocytochemical localization of glucose 6-phosphatase and cytosolic phosphoenolpyruvate carboxykinase in gluconeogenic tissues reveals unsuspected metabolic zonation
    Fabienne Rajas
    INSERM, U885, Lyon, F 69008, France
    Histochem Cell Biol 127:555-65. 2007
    ..localization of Glucose-6-phosphatase (Glc6Pase) and cytosolic form of the phosphoenolpyruvate carboxykinase (PEPCK-C) in the digestive system (liver, small intestine and pancreas) and the kidney...