SRSF1

Summary

Gene Symbol: SRSF1
Description: serine and arginine rich splicing factor 1
Alias: ASF, SF2, SF2p33, SFRS1, SRp30a, serine/arginine-rich splicing factor 1, SR splicing factor 1, alternative-splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit, splicing factor 2, splicing factor, arginine/serine-rich, 30-KD, A
Species: human
Products:     SRSF1

Top Publications

  1. Zuo P, Manley J. Functional domains of the human splicing factor ASF/SF2. EMBO J. 1993;12:4727-37 pubmed
    The human splicing factor ASF/SF2 displays two predominant activities in in vitro splicing assays: (i) it is an essential factor apparently required for all splices and (ii) it is able to switch utilization of alternative 5' splice sites ..
  2. Sanford J, Ellis J, Cazalla D, Caceres J. Reversible phosphorylation differentially affects nuclear and cytoplasmic functions of splicing factor 2/alternative splicing factor. Proc Natl Acad Sci U S A. 2005;102:15042-7 pubmed
    ..Here, we show that endogenous, cytoplasmic splicing factor 2/alternative splicing factor (SF2/ASF) associated with the translation machinery is hypophosphorylated, ..
  3. Moulton V, Tsokos G. Alternative splicing factor/splicing factor 2 regulates the expression of the zeta subunit of the human T cell receptor-associated CD3 complex. J Biol Chem. 2010;285:12490-6 pubmed publisher
    ..by a CD3zeta 3'-UTR oligonucleotide, which identified the splicing protein alternative splicing factor/splicing factor 2 (ASF/SF2)...
  4. Sun D, Novotny M, Bulek K, Liu C, Li X, Hamilton T. Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF). Nat Immunol. 2011;12:853-60 pubmed publisher
    ..adaptor Act1, the adaptors TRAF2 or TRAF5 and the splicing factor SF2 (also known as alternative splicing factor (ASF)). TRAF2 and TRAF5 were necessary for IL-17 to signal the stabilization of CXCL1 mRNA...
  5. Huang Y, Gattoni R, Stevenin J, Steitz J. SR splicing factors serve as adapter proteins for TAP-dependent mRNA export. Mol Cell. 2003;11:837-43 pubmed
    ..Thus, multiple adapters including SR proteins most likely cooperate to recruit multiple copies of TAP/NXF1 for efficient mRNA export. ..
  6. Gonçalves V, Matos P, Jordan P. Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways. Hum Mol Genet. 2009;18:3696-707 pubmed publisher
    ..Here we describe that ASF/SF2 and SRp20 are two antagonistic splicing factors regulating Rac1b expression in colorectal tumor cells...
  7. Ghigna C, Giordano S, Shen H, Benvenuto F, Castiglioni F, Comoglio P, et al. Cell motility is controlled by SF2/ASF through alternative splicing of the Ron protooncogene. Mol Cell. 2005;20:881-90 pubmed
    ..parallels the relative abundance of DeltaRon mRNA and depends on a sequence directly bound by splicing factor SF2/ASF. Overexpression and RNAi experiments demonstrate that SF2/ASF, by controlling the production of DeltaRon, activates ..
  8. Sanford J, Wang X, Mort M, Vanduyn N, Cooper D, Mooney S, et al. Splicing factor SFRS1 recognizes a functionally diverse landscape of RNA transcripts. Genome Res. 2009;19:381-94 pubmed publisher
    ..we define the specificity of a central regulator of pre-mRNA splicing, the conserved, essential splicing factor SFRS1. Cross-linking immunoprecipitation and high-throughput sequencing (CLIP-seq) identified 23,632 binding sites for ..
  9. Karni R, de Stanchina E, Lowe S, Sinha R, Mu D, Krainer A. The gene encoding the splicing factor SF2/ASF is a proto-oncogene. Nat Struct Mol Biol. 2007;14:185-93 pubmed
    ..that the splicing factor SF2/ASF is upregulated in various human tumors, in part due to amplification of its gene, SFRS1. Moreover, slight overexpression of SF2/ASF is sufficient to transform immortal rodent fibroblasts, which form ..

More Information

Publications71

  1. Michlewski G, Sanford J, Caceres J. The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1. Mol Cell. 2008;30:179-89 pubmed publisher
    The SR protein SF2/ASF has been initially characterized as a splicing factor but has also been shown to mediate postsplicing activities such as mRNA export and translation...
  2. Leu S, Lin Y, Wu C, Ouyang P. Loss of Pnn expression results in mouse early embryonic lethality and cellular apoptosis through SRSF1-mediated alternative expression of Bcl-xS and ICAD. J Cell Sci. 2012;125:3164-72 pubmed publisher
    ..RT-PCR analysis showed that alternative splicing patterns of SRSF1 as well as of apoptosis-related genes Bcl-x and ICAD were altered, and expression levels of Bim isoforms were ..
  3. Gui J, Lane W, Fu X. A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature. 1994;369:678-82 pubmed
  4. Cazalla D, Newton K, Caceres J. A novel SR-related protein is required for the second step of Pre-mRNA splicing. Mol Cell Biol. 2005;25:2969-80 pubmed
    ..SRrp53 also regulates alternative splicing in a concentration-dependent manner. Taken together, these results suggest that SRrp53 is a novel SR-related protein that has a role both in constitutive and in alternative splicing. ..
  5. Zhang Z, Krainer A. Involvement of SR proteins in mRNA surveillance. Mol Cell. 2004;16:597-607 pubmed
    ..Our data argue against a universal role of PTCs in regulating pre-mRNA splicing and reveal an additional function of SR proteins in eukaryotic gene expression. ..
  6. McPhillips M, Veerapraditsin T, Cumming S, Karali D, Milligan S, Boner W, et al. SF2/ASF binds the human papillomavirus type 16 late RNA control element and is regulated during differentiation of virus-infected epithelial cells. J Virol. 2004;78:10598-605 pubmed
    ..Here, we show that the SR protein SF2/ASF is part of a complex that forms upon the 79-nucleotide negative regulatory element (NRE) that is thought to be ..
  7. Li X, Wang J, Manley J. Loss of splicing factor ASF/SF2 induces G2 cell cycle arrest and apoptosis, but inhibits internucleosomal DNA fragmentation. Genes Dev. 2005;19:2705-14 pubmed
    b>ASF/SF2 is an SR protein splicing factor that participates in constitutive and alternative pre-mRNA splicing and is essential for cell viability...
  8. Massiello A, Chalfant C. SRp30a (ASF/SF2) regulates the alternative splicing of caspase-9 pre-mRNA and is required for ceramide-responsiveness. J Lipid Res. 2006;47:892-7 pubmed
    ..The downregulation of SRp30a in A549 cells by RNA interference technology resulted in an increase in the caspase-9b splice variant, with a ..
  9. Cazalla D, Zhu J, Manche L, Huber E, Krainer A, Caceres J. Nuclear export and retention signals in the RS domain of SR proteins. Mol Cell Biol. 2002;22:6871-82 pubmed
    ..We analyzed mutant versions of human SF2/ASF in which the natural RS repeats were replaced by RD or RE repeats and compared the splicing and subcellular ..
  10. Shepard P, Hertel K. The SR protein family. Genome Biol. 2009;10:242 pubmed publisher
    ..These activities point up the importance of SR proteins during the regulation of mRNA metabolism. ..
  11. Cao W, Garcia Blanco M. A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding. J Biol Chem. 1998;273:20629-35 pubmed
    ..ASF/SF2, SC35, and other members of the serine/arginine family, interact with the 70k protein of the U1 small nuclear ..
  12. Caceres J, Screaton G, Krainer A. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm. Genes Dev. 1998;12:55-66 pubmed
    ..We show here that whereas some human SR proteins are confined to the nucleus, three of them-SF2/ASF, SRp20, and 9G8-shuttle rapidly and continuously between the nucleus and the cytoplasm...
  13. Tripathi V, Ellis J, Shen Z, Song D, Pan Q, Watt A, et al. The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation. Mol Cell. 2010;39:925-38 pubmed publisher
    ..Taken together, our results suggest that MALAT1 regulates AS by modulating the levels of active SR proteins. Our results further highlight the role for an nrRNA in the regulation of gene expression. ..
  14. Shultz J, Goehe R, Murudkar C, Wijesinghe D, Mayton E, Massiello A, et al. SRSF1 regulates the alternative splicing of caspase 9 via a novel intronic splicing enhancer affecting the chemotherapeutic sensitivity of non-small cell lung cancer cells. Mol Cancer Res. 2011;9:889-900 pubmed publisher
    ..Specifically, the pre-mRNA sequence of caspase 9 was analyzed for RNA cis-elements known to interact with SRSF1, a required enhancer for caspase 9 RNA splicing...
  15. Kohtz J, Jamison S, Will C, Zuo P, Luhrmann R, Garcia Blanco M, et al. Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors. Nature. 1994;368:119-24 pubmed
    ..Now a human protein essential for splicing in vitro, called alternative splicing factor/splicing factor 2, is shown to cooperate with the U1 snRNP particle in binding pre-mRNA...
  16. Tacke R, Manley J. The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 1995;14:3540-51 pubmed
    ASF/SF2 and SC35 belong to a highly conserved family of nuclear proteins that are both essential for splicing of pre-mRNA in vitro and are able to influence selection of alternative splice sites...
  17. Mole S, Milligan S, Graham S. Human papillomavirus type 16 E2 protein transcriptionally activates the promoter of a key cellular splicing factor, SF2/ASF. J Virol. 2009;83:357-67 pubmed publisher
    ..Previously, we demonstrated that upon epithelial differentiation, HPV16 infection upregulates hnRNP A1 and SF2/ASF, both key factors in alternative splicing regulation...
  18. Karni R, Hippo Y, Lowe S, Krainer A. The splicing-factor oncoprotein SF2/ASF activates mTORC1. Proc Natl Acad Sci U S A. 2008;105:15323-7 pubmed publisher
    The splicing factor SF2/ASF is an oncoprotein that is up-regulated in many cancers and can transform immortal rodent fibroblasts when slightly overexpressed...
  19. Ezponda T, Pajares M, Agorreta J, Echeveste J, Lopez Picazo J, Torre W, et al. The oncoprotein SF2/ASF promotes non-small cell lung cancer survival by enhancing survivin expression. Clin Cancer Res. 2010;16:4113-25 pubmed publisher
    SF2/ASF is a splicing factor recently described as an oncoprotein...
  20. Shultz J, Goehe R, Wijesinghe D, Murudkar C, Hawkins A, Shay J, et al. Alternative splicing of caspase 9 is modulated by the phosphoinositide 3-kinase/Akt pathway via phosphorylation of SRp30a. Cancer Res. 2010;70:9185-96 pubmed publisher
    ..mediate exclusion of the exon 3,4,5,6 cassette of Casp9 via the phosphorylation state of the RNA splicing factor SRp30a via serines 199, 201, 227, and 234...
  21. Li X, Manley J. Inactivation of the SR protein splicing factor ASF/SF2 results in genomic instability. Cell. 2005;122:365-78 pubmed
    ..Here we provide evidence that a prototypical SR protein, ASF/SF2, is unexpectedly required for maintenance of genomic stability...
  22. Das S, Anczukow O, Akerman M, Krainer A. Oncogenic splicing factor SRSF1 is a critical transcriptional target of MYC. Cell Rep. 2012;1:110-7 pubmed
    The SR protein splicing factor SRSF1 is a potent proto-oncogene that is frequently upregulated in cancer. Here, we show that SRSF1 is a direct target of the transcription factor oncoprotein MYC...
  23. Moulton V, Grammatikos A, Fitzgerald L, Tsokos G. Splicing factor SF2/ASF rescues IL-2 production in T cells from systemic lupus erythematosus patients by activating IL-2 transcription. Proc Natl Acad Sci U S A. 2013;110:1845-50 pubmed publisher
    ..We recently showed that the serine arginine protein splicing factor 2/alternative splicing factor (SF2/ASF) enhances the expression of CD3? chain by limiting the production of an ..
  24. Krainer A, Mayeda A, Kozak D, Binns G. Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators. Cell. 1991;66:383-94 pubmed
    b>SF2 is a protein factor essential for constitutive pre-mRNA splicing in HeLa cell extracts and also activates proximal alternative 5' splice sites in a concentration-dependent manner...
  25. Lai M, Tarn W. Hypophosphorylated ASF/SF2 binds TAP and is present in messenger ribonucleoproteins. J Biol Chem. 2004;279:31745-9 pubmed
    ..receptor TAP associates preferentially with the hypophosphorylated form of shuttling SR proteins, including ASF/SF2. Overexpression of ASF induced subnuclear relocalization of TAP to SR protein-enriched nuclear speckles, suggesting ..
  26. Ropers D, Ayadi L, Gattoni R, Jacquenet S, Damier L, Branlant C, et al. Differential effects of the SR proteins 9G8, SC35, ASF/SF2, and SRp40 on the utilization of the A1 to A5 splicing sites of HIV-1 RNA. J Biol Chem. 2004;279:29963-73 pubmed
    ..b>ASF/SF2 strongly activates site A2 and to a lesser extent site A1...
  27. Koizumi J, Okamoto Y, Onogi H, Mayeda A, Krainer A, Hagiwara M. The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs). J Biol Chem. 1999;274:11125-31 pubmed
    ..The C-terminal arginine-serine domain of these proteins, such as SF2/ASF, mediates protein-protein interactions and is phosphorylated in vivo...
  28. Fu Y, Huang B, Shi Z, Han J, Wang Y, Huangfu J, et al. SRSF1 and SRSF9 RNA binding proteins promote Wnt signalling-mediated tumorigenesis by enhancing ?-catenin biosynthesis. EMBO Mol Med. 2013;5:737-50 pubmed publisher
    ..Overexpression of SRSF1 and SRSF9 promote ?-catenin accumulation via the recruitment of ?-catenin mRNA and by enhancing its translation in ..
  29. Maita H, Kitaura H, Ariga H, Iguchi Ariga S. CIR, a corepressor of CBF1, binds to PAP-1 and effects alternative splicing. Exp Cell Res. 2005;303:375-87 pubmed
    ..and studied their subcellular localizations and interactions with PAP-1 and other SR proteins, including SC35, SF2/ASF, and U2AF35...
  30. Tripathi V, Song D, Zong X, Shevtsov S, Hearn S, Fu X, et al. SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles. Mol Biol Cell. 2012;23:3694-706 pubmed publisher
    ..Depletion of SRSF1 in human cells compromises the association of splicing factors to nuclear speckles and influences the levels and ..
  31. Ge H, Zuo P, Manley J. Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators. Cell. 1991;66:373-82 pubmed
    We described previously the purification of a human protein, called alternative splicing factor (ASF), that can switch utilization of alternative 5' splice sites in an SV40 early pre-mRNA...
  32. Wang H, Lin W, Dyck J, Yeakley J, Songyang Z, Cantley L, et al. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J Cell Biol. 1998;140:737-50 pubmed
    ..Phosphorylation of an SR protein (ASF/SF2) by either SRPK1 or 2 enhanced its interaction with another RS domain-containing protein (U1 70K), and ..
  33. Sinha R, Allemand E, Zhang Z, Karni R, Myers M, Krainer A. Arginine methylation controls the subcellular localization and functions of the oncoprotein splicing factor SF2/ASF. Mol Cell Biol. 2010;30:2762-74 pubmed publisher
    ..The SR protein SF2/ASF is an oncoprotein that functions in pre-mRNA splicing, with additional roles in other posttranscriptional and ..
  34. Xiao S, Manley J. Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2. EMBO J. 1998;17:6359-67 pubmed
    ..We reported previously that phosphorylation of the SR protein ASF/SF2 enhances its interaction with the U1 snRNP-specific 70K protein and is required for the protein to function in ..
  35. Lai M, Lin R, Huang S, Tsai C, Tarn W. A human importin-beta family protein, transportin-SR2, interacts with the phosphorylated RS domain of SR proteins. J Biol Chem. 2000;275:7950-7 pubmed
    ..Thus, our results likely reflect a role of TRN-SR2 in the cellular trafficking of phosphorylated SR proteins. ..
  36. Zhu J, Mayeda A, Krainer A. Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins. Mol Cell. 2001;8:1351-61 pubmed
    ..We investigated how ESS3 in HIV-1 tat exon 3 blocks splicing promoted by one SR protein (SC35) but not another (SF2/ASF)...
  37. Nogues G, Kadener S, Cramer P, Bentley D, Kornblihtt A. Transcriptional activators differ in their abilities to control alternative splicing. J Biol Chem. 2002;277:43110-4 pubmed
    ..Rapid, highly processive transcription favors EDI exon skipping, whereas slower, less processive transcription favors inclusion. ..
  38. Tronchere H, Wang J, Fu X. A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA. Nature. 1997;388:397-400 pubmed
    ..Therefore, Urp and U2AF35 may independently position RS-domain-containing factors within spliceosomes. ..
  39. Kataoka N, Bachorik J, Dreyfuss G. Transportin-SR, a nuclear import receptor for SR proteins. J Cell Biol. 1999;145:1145-52 pubmed
    ..SR protein import receptor, termed transportin-SR (TRN-SR), binds specifically and directly to the RS domains of ASF/SF2 and SC35 as well as several other SR proteins...
  40. Zahler A, Lane W, Stolk J, Roth M. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 1992;6:837-47 pubmed
    ..In four of the family members a repeated protein sequence that encompasses an RNA recognition motif was observed. We discuss the potential role of this highly conserved, functionally related set of proteins in pre-mRNA splicing. ..
  41. Umehara H, Nishii Y, Morishima M, Kakehi Y, Kioka N, Amachi T, et al. Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear protein CROP/Luc7A. Biochem Biophys Res Commun. 2003;301:324-9 pubmed
    ..The yeast two-hybrid system revealed that CROP interacted with SF2/ASF, an SR protein involved in RNA splicing, as well as CROP itself...
  42. Sikora D, Greco Stewart V, Miron P, Pelchat M. The hepatitis delta virus RNA genome interacts with eEF1A1, p54(nrb), hnRNP-L, GAPDH and ASF/SF2. Virology. 2009;390:71-8 pubmed publisher
    ..Co-immunoprecipitation was used to confirm the interactions of eEF1A1, p54(nrb), hnRNP-L, GAPDH and ASF/SF2 with the right terminal stem-loop domain of HDV genomic RNA in vitro, and with both polarities of HDV RNA within ..
  43. Ngo J, Giang K, Chakrabarti S, Ma C, Huynh N, Hagopian J, et al. A sliding docking interaction is essential for sequential and processive phosphorylation of an SR protein by SRPK1. Mol Cell. 2008;29:563-76 pubmed publisher
    The 2.9 A crystal structure of the core SRPK1:ASF/SF2 complex reveals that the N-terminal half of the basic RS domain of ASF/SF2, which is destined to be phosphorylated, is bound to an acidic docking groove of SRPK1 distal to the active ..
  44. Liu X, Mayeda A, Tao M, Zheng Z. Exonic splicing enhancer-dependent selection of the bovine papillomavirus type 1 nucleotide 3225 3' splice site can be rescued in a cell lacking splicing factor ASF/SF2 through activation of the phosphatidylinositol 3-kinase/Akt pathway. J Virol. 2003;77:2105-15 pubmed
    ..Two exonic splicing enhancers, each containing two ASF/SF2 (alternative splicing factor/splicing factor 2) binding sites, are located between the two 3' splice sites and have been identified as regulating ..
  45. Wu H, Sun S, Tu K, Gao Y, Xie B, Krainer A, et al. A splicing-independent function of SF2/ASF in microRNA processing. Mol Cell. 2010;38:67-77 pubmed publisher
    ..we identified 40 miRNAs that are differentially expressed upon ectopic overexpression of the splicing factor SF2/ASF. Here we show that SF2/ASF and one of its upregulated microRNAs (miR-7) can form a negative feedback loop: SF2/ASF ..
  46. Anczukow O, Rosenberg A, Akerman M, Das S, Zhan L, Karni R, et al. The splicing factor SRSF1 regulates apoptosis and proliferation to promote mammary epithelial cell transformation. Nat Struct Mol Biol. 2012;19:220-8 pubmed publisher
    The splicing-factor oncoprotein SRSF1 (also known as SF2/ASF or ASF/SF2) is upregulated in breast cancers. We investigated the ability of SRSF1 to transform human and mouse mammary epithelial cells in vivo and in vitro...
  47. Colwill K, Pawson T, Andrews B, Prasad J, Manley J, Bell J, et al. The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution. EMBO J. 1996;15:265-75 pubmed
    ..In vitro, Clk/Sty efficiently phosphorylated the SR family member ASF/SF2 on serine residues located within its serine/arginine-rich region (the RS domain)...
  48. Lareau L, Inada M, Green R, Wengrod J, Brenner S. Unproductive splicing of SR genes associated with highly conserved and ultraconserved DNA elements. Nature. 2007;446:926-9 pubmed
    ..We find that unproductive splicing associated with conserved regions has arisen independently in different SR genes, suggesting that splicing factors may readily acquire this form of regulation. ..
  49. Wu J, Maniatis T. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing. Cell. 1993;75:1061-70 pubmed
    ..We report that the splicing factors SC35 and SF2/ASF specifically interact with both the integral U1 small nuclear ribonucleoprotein (snRNP U1-70K) and with the 35 kd ..
  50. Fregoso O, Das S, Akerman M, Krainer A. Splicing-factor oncoprotein SRSF1 stabilizes p53 via RPL5 and induces cellular senescence. Mol Cell. 2013;50:56-66 pubmed publisher
    ..Therefore, the cell has many safeguards against such misregulation. We report that the oncogenic splicing factor SRSF1, which is overexpressed in many cancers, stabilizes the tumor suppressor protein p53 by abrogating its MDM2-..
  51. Caputi M, Freund M, Kammler S, Asang C, Schaal H. A bidirectional SF2/ASF- and SRp40-dependent splicing enhancer regulates human immunodeficiency virus type 1 rev, env, vpu, and nef gene expression. J Virol. 2004;78:6517-26 pubmed
    ..The ESE is localized downstream of the vpu-, env-, and nef-specific 3' splice site no. 5. SF2/ASF and SRp40 activate the ESE and are required for efficient 3' splice site usage and binding of the U1 snRNP to ..
  52. Verduci L, Simili M, Rizzo M, Mercatanti A, Evangelista M, Mariani L, et al. MicroRNA (miRNA)-mediated interaction between leukemia/lymphoma-related factor (LRF) and alternative splicing factor/splicing factor 2 (ASF/SF2) affects mouse embryonic fibroblast senescence and apoptosis. J Biol Chem. 2010;285:39551-63 pubmed publisher
    ..Both miRNAs are predicted to target alternative splicing factor/splicing factor 2 (ASF/SF2), a serine/arginine protein essential for cell viability...
  53. Pelisch F, Gerez J, Druker J, Schor I, Muñoz M, Risso G, et al. The serine/arginine-rich protein SF2/ASF regulates protein sumoylation. Proc Natl Acad Sci U S A. 2010;107:16119-24 pubmed publisher
    ..Here, we show that the serine/arginine-rich protein SF2/ASF, a factor involved in splicing regulation and other RNA metabolism-related processes, is a regulator of the ..
  54. Barnard D, Patton J. Identification and characterization of a novel serine-arginine-rich splicing regulatory protein. Mol Cell Biol. 2000;20:3049-57 pubmed
    ..even inhibit the in vitro and in vivo activation of specific splice sites by a subset of SR proteins, including ASF/SF2, SC35, and SRp55. In contrast, p86 activates splicing in the presence of SRp20...
  55. Somberg M, Schwartz S. Multiple ASF/SF2 sites in the human papillomavirus type 16 (HPV-16) E4-coding region promote splicing to the most commonly used 3'-splice site on the HPV-16 genome. J Virol. 2010;84:8219-30 pubmed publisher
    ..human papillomavirus type 16 (HPV-16) mRNAs expressing the viral oncogenes E6 and E7 are regulated by cellular ASF/SF2, itself defined as a proto-oncogene and overexpressed in cervical cancer cells...
  56. Shaw S, Chakrabarti S, Ghosh G, Krainer A. Deletion of the N-terminus of SF2/ASF permits RS-domain-independent pre-mRNA splicing. PLoS ONE. 2007;2:e854 pubmed
    ..However, we previously reported that the RS domain of the SR protein SF2/ASF is dispensable for in vitro splicing of some pre-mRNAs...
  57. Sun S, Zhang Z, Sinha R, Karni R, Krainer A. SF2/ASF autoregulation involves multiple layers of post-transcriptional and translational control. Nat Struct Mol Biol. 2010;17:306-12 pubmed publisher
    ..Splicing factor, arginine/serine-rich 1 (SFRS1), the gene encoding SF2/ASF, is a potent proto-oncogene with abnormal expression in many tumors...
  58. Loomis R, Naoe Y, Parker J, Savic V, Bozovsky M, Macfarlan T, et al. Chromatin binding of SRp20 and ASF/SF2 and dissociation from mitotic chromosomes is modulated by histone H3 serine 10 phosphorylation. Mol Cell. 2009;33:450-61 pubmed publisher
    ..We report here that two SR protein splicing factors, SRp20 and ASF/SF2, associate with interphase chromatin, are released from hyperphosphorylated mitotic chromosomes, but reassociate ..
  59. Sanford J, Coutinho P, Hackett J, Wang X, Ranahan W, Caceres J. Identification of nuclear and cytoplasmic mRNA targets for the shuttling protein SF2/ASF. PLoS ONE. 2008;3:e3369 pubmed publisher
    ..that ectopic expression of SF2/ASF regulates the splicing and polysome association of transcripts derived from the SFRS1, PABC1, NETO2 and ENSA genes...
  60. Colwill K, Feng L, Yeakley J, Gish G, Caceres J, Pawson T, et al. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J Biol Chem. 1996;271:24569-75 pubmed
    ..two kinases phosphorylate the same SR proteins in vitro, but that SRPK1 has the higher specific activity toward ASF/SF2. SRPK1, like Clk/Sty, phosphorylates ASF/SF2 in vitro on sites that are also phosphorylated in vivo...
  61. Blaustein M, Quadrana L, Risso G, Mata M, Pelisch F, Srebrow A. SF2/ASF regulates proteomic diversity by affecting the balance between translation initiation mechanisms. J Cell Biochem. 2009;107:826-33 pubmed publisher
    ..have been described for a subset of shuttling serine/arginine-rich splicing regulatory proteins, among them SF2/ASF...
  62. Hagopian J, Ma C, Meade B, Albuquerque C, Ngo J, Ghosh G, et al. Adaptable molecular interactions guide phosphorylation of the SR protein ASF/SF2 by SRPK1. J Mol Biol. 2008;382:894-909 pubmed publisher
    The SR (arginine-serine rich) protein ASF/SF2 (also called human alternative splicing factor), an essential splicing factor, contains two functional modules consisting of tandem RNA recognition motifs (RRMs; RRM1-RRM2) and a C-terminal ..