SMRT

Summary

Gene Symbol: SMRT
Description: nuclear receptor corepressor 2
Alias: CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1, nuclear receptor corepressor 2, CTG repeat protein 26, T3 receptor-associating factor, silencing mediator for retinoid and thyroid hormone receptors, thyroid-, retinoic-acid-receptor-associated corepressor
Species: human
Products:     SMRT

Top Publications

  1. Yu J, Li Y, Ishizuka T, Guenther M, Lazar M. A SANT motif in the SMRT corepressor interprets the histone code and promotes histone deacetylation. EMBO J. 2003;22:3403-10 pubmed
    Nuclear receptor corepressors SMRT (silencing mediator of retinoid and thyroid receptors) and N-CoR (nuclear receptor corepressor) recruit histone deacetylase (HDAC) activity to targeted regions of chromatin...
  2. Hörlein A, Näär A, Heinzel T, Torchia J, Gloss B, Kurokawa R, et al. Ligand-independent repression by the thyroid hormone receptor mediated by a nuclear receptor co-repressor. Nature. 1995;377:397-404 pubmed
  3. Shink E, Harvey M, Tremblay M, Raymond C, Labbé M, Gagné B, et al. Exclusion of non-synonymous SNPs and a polyglutamine tract in SMRT/N-CoR2 as common deleterious mutation for bipolar disorder in the Sagnenay-Lac-St-Jean population. Am J Med Genet B Neuropsychiatr Genet. 2005;134B:10-2 pubmed
    ..31 chromosomal region. Here, we investigated the possible involvement of the SMRT/N-CoR2 gene, which encodes for the nuclear receptor co-repressor 2...
  4. Hoberg J, Yeung F, Mayo M. SMRT derepression by the IkappaB kinase alpha: a prerequisite to NF-kappaB transcription and survival. Mol Cell. 2004;16:245-55 pubmed
    ..Here, we demonstrate that NF-kappaB transcription requires IKKalpha to phosphorylate SMRT on chromatin, stimulating the exchange of corepressor for coactivator complexes...
  5. Kim J, Son Y, Lee Y. Involvement of SMRT corepressor in transcriptional repression by the vitamin D receptor. Mol Endocrinol. 2009;23:251-64 pubmed publisher
    ..the unliganded nuclear receptor generally recruits the silencing mediator of retinoid and thyroid hormone receptor (SMRT)/nuclear receptor corepressor via its direct association with the conserved motif within bipartite nuclear receptor-..
  6. Tsai C, Kao H, Mitzutani A, Banayo E, Rajan H, McKeown M, et al. Ataxin 1, a SCA1 neurodegenerative disorder protein, is functionally linked to the silencing mediator of retinoid and thyroid hormone receptors. Proc Natl Acad Sci U S A. 2004;101:4047-52 pubmed
    ..In this study, we demonstrate that Atx1 interacts with the transcriptional corepressor SMRT (silencing mediator of retinoid and thyroid hormone receptors) and with histone deacetylase 3...
  7. Guenther M, Lane W, Fischle W, Verdin E, Lazar M, Shiekhattar R. A core SMRT corepressor complex containing HDAC3 and TBL1, a WD40-repeat protein linked to deafness. Genes Dev. 2000;14:1048-57 pubmed
    The corepressor SMRT mediates repression by thyroid hormone receptor (TR) as well as other nuclear hormone receptors and transcription factors. Here we report the isolation of a novel SMRT-containing complex from HeLa cells...
  8. Li Y, Kao G, Garcia B, Shabanowitz J, Hunt D, Qin J, et al. A novel histone deacetylase pathway regulates mitosis by modulating Aurora B kinase activity. Genes Dev. 2006;20:2566-79 pubmed
  9. Smith C, Nawaz Z, O Malley B. Coactivator and corepressor regulation of the agonist/antagonist activity of the mixed antiestrogen, 4-hydroxytamoxifen. Mol Endocrinol. 1997;11:657-66 pubmed
    ..was measured in the absence or presence of expression vectors for SRC-1 (steroid receptor coactivator-1) or SMRT (silencing mediator of retinoic acid and thyroid hormone receptors)...

More Information

Publications89

  1. Yoon H, Chan D, Reynolds A, Qin J, Wong J. N-CoR mediates DNA methylation-dependent repression through a methyl CpG binding protein Kaiso. Mol Cell. 2003;12:723-34 pubmed
    ..Thus, our data demonstrate a critical role for a methyl CpG binding protein in mediating DNA methylation-dependent repression and reveal the mechanism by which it represses transcription. ..
  2. Varlakhanova N, Hahm J, Privalsky M. Regulation of SMRT corepressor dimerization and composition by MAP kinase phosphorylation. Mol Cell Endocrinol. 2011;332:180-8 pubmed publisher
    The SMRT (Silencing Mediator of Retinoid and Thyroid hormone receptors) corepressor mediates gene repression by nuclear receptors and other transcriptional factors...
  3. Li J, Wang J, Nawaz Z, Liu J, Qin J, Wong J. Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3. EMBO J. 2000;19:4342-50 pubmed
    We present evidence that both corepressors SMRT and N-CoR exist in large protein complexes with estimated sizes of 1.5-2 MDa in HeLa nuclear extracts...
  4. Chen J, Evans R. A transcriptional co-repressor that interacts with nuclear hormone receptors. Nature. 1995;377:454-7 pubmed
    ..Here we identify a receptor-interacting factor, SMRT, as a silencing mediator (co-repressor) for retinoid and thyroid-hormone receptors...
  5. Guan H, Ishizuka T, Chui P, Lehrke M, Lazar M. Corepressors selectively control the transcriptional activity of PPARgamma in adipocytes. Genes Dev. 2005;19:453-61 pubmed
    ..Thus, selective modulation of adipocyte PPARgamma target genes by TZDs involves the dissociation of corepressors by direct and indirect mechanisms. ..
  6. Guenther M, Yu J, Kao G, Yen T, Lazar M. Assembly of the SMRT-histone deacetylase 3 repression complex requires the TCP-1 ring complex. Genes Dev. 2002;16:3130-5 pubmed
    ..Histone deacetylase 3 (HDAC3) requires the nuclear receptor corepressor SMRT for HDAC enzyme activity...
  7. Huang E, Zhang J, Miska E, Guenther M, Kouzarides T, Lazar M. Nuclear receptor corepressors partner with class II histone deacetylases in a Sin3-independent repression pathway. Genes Dev. 2000;14:45-54 pubmed
    Transcriptional repression mediated by corepressors N-CoR and SMRT is a critical function of nuclear hormone receptors, and is dysregulated in human myeloid leukemias...
  8. Wen Y, Perissi V, Staszewski L, Yang W, Krones A, Glass C, et al. The histone deacetylase-3 complex contains nuclear receptor corepressors. Proc Natl Acad Sci U S A. 2000;97:7202-7 pubmed
    ..These results provide a possible molecular mechanism for HDAC3 regulation and argue that N-CoR is a platform in which distinct domains can interact with most of the known HDACs. ..
  9. Guenther M, Barak O, Lazar M. The SMRT and N-CoR corepressors are activating cofactors for histone deacetylase 3. Mol Cell Biol. 2001;21:6091-101 pubmed
    ..In vivo, HDAC3 forms a stable complex with the SMRT corepressor...
  10. Yoon H, Chan D, Huang Z, Li J, Fondell J, Qin J, et al. Purification and functional characterization of the human N-CoR complex: the roles of HDAC3, TBL1 and TBLR1. EMBO J. 2003;22:1336-46 pubmed
    Corepressors N-CoR and SMRT participate in diverse repression pathways and exist in large protein complexes including HDAC3, TBL1 and TBLR1. However, the roles of these proteins in SMRT-N-CoR complex function are largely unknown...
  11. Zhang J, Kalkum M, Chait B, Roeder R. The N-CoR-HDAC3 nuclear receptor corepressor complex inhibits the JNK pathway through the integral subunit GPS2. Mol Cell. 2002;9:611-23 pubmed
    The corepressors N-CoR and SMRT partner with histone deacetylases (HDACs) in diverse repression pathways. We report here that GPS2, a protein involved in intracellular signaling, is an integral subunit of the N-CoR-HDAC3 complex...
  12. Codina A, Love J, Li Y, Lazar M, Neuhaus D, Schwabe J. Structural insights into the interaction and activation of histone deacetylase 3 by nuclear receptor corepressors. Proc Natl Acad Sci U S A. 2005;102:6009-14 pubmed
    b>SMRT (silencing mediator of retinoid acid and thyroid hormone receptor) and NCoR (nuclear receptor corepressor) are transcriptional corepressors that play an essential role in the regulation of development and metabolism...
  13. Stanya K, Liu Y, Means A, Kao H. Cdk2 and Pin1 negatively regulate the transcriptional corepressor SMRT. J Cell Biol. 2008;183:49-61 pubmed publisher
    Silencing mediator for retinoic acid and thyroid hormone receptor (SMRT) is a transcriptional corepressor that participates in diverse signaling pathways and human diseases. However, regulation of SMRT stability remains largely unexplored...
  14. Khanim F, Gommersall L, Wood V, Smith K, Montalvo L, O Neill L, et al. Altered SMRT levels disrupt vitamin D3 receptor signalling in prostate cancer cells. Oncogene. 2004;23:6712-25 pubmed
    ..expression in a panel of nonmalignant and malignant cell lines and primary cultures, and found frequently elevated SMRT corepressor mRNA expression often associated with reduced sensitivity to 1alpha,25-dihydroxyvitamin D(3) (1alpha,25(..
  15. Green A, Burney C, Granger C, Paish E, El Sheikh S, Rakha E, et al. The prognostic significance of steroid receptor co-regulators in breast cancer: co-repressor NCOR2/SMRT is an independent indicator of poor outcome. Breast Cancer Res Treat. 2008;110:427-37 pubmed
    ..study investigates the pattern of expression of the steroid receptor co-regulators NCOA1/SRC1, NCOA3/RAC3, NCOR2/SMRT, and CBP/p300 in breast cancer...
  16. Sande S, Privalsky M. Identification of TRACs (T3 receptor-associating cofactors), a family of cofactors that associate with, and modulate the activity of, nuclear hormone receptors. Mol Endocrinol. 1996;10:813-25 pubmed
    ..Certain TRAC forms attenuate target gene expression and may serve as corepressors, whereas other TRAC family members appear to ..
  17. Fischle W, Dequiedt F, Hendzel M, Guenther M, Lazar M, Voelter W, et al. Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR. Mol Cell. 2002;9:45-57 pubmed
    ..Here, we show that the catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor N-CoR/SMRT. All experimental conditions leading to the suppression of HDAC4 binding to SMRT/N-CoR and to HDAC3 result in the ..
  18. Keeton E, Brown M. Cell cycle progression stimulated by tamoxifen-bound estrogen receptor-alpha and promoter-specific effects in breast cancer cells deficient in N-CoR and SMRT. Mol Endocrinol. 2005;19:1543-54 pubmed
    ..Tamoxifen-bound ERalpha associates with nuclear receptor corepressor (N-CoR) and silencing mediator for retinoid and thyroid hormone receptors (SMRT) at certain target genes...
  19. Ordentlich P, Downes M, Xie W, Genin A, Spinner N, Evans R. Unique forms of human and mouse nuclear receptor corepressor SMRT. Proc Natl Acad Sci U S A. 1999;96:2639-44 pubmed
    ..groups demonstrate that this complex is recruited to nuclear receptors through the highly related corepressors SMRT (silencing mediator of retinoid acid and thyroid hormone receptor) and N-CoR (nuclear receptor corepressor)...
  20. Gao C, Li X, Lam M, Liu Y, Chakraborty S, Kao H. CRM1 mediates nuclear export of HDAC7 independently of HDAC7 phosphorylation and association with 14-3-3s. FEBS Lett. 2006;580:5096-104 pubmed
    ..Taken together, these results strongly suggest that CRM1 mediated-nuclear export of HDAC7 is independent of HDAC7 phosphorylation and its association with 14-3-3s. ..
  21. Haiman C, Garcia R, Hsu C, Xia L, Ha H, Sheng X, et al. Screening and association testing of common coding variation in steroid hormone receptor co-activator and co-repressor genes in relation to breast cancer risk: the Multiethnic Cohort. BMC Cancer. 2009;9:43 pubmed publisher
  22. Fujita T, Kobayashi Y, Wada O, Tateishi Y, Kitada L, Yamamoto Y, et al. Full activation of estrogen receptor alpha activation function-1 induces proliferation of breast cancer cells. J Biol Chem. 2003;278:26704-14 pubmed
    ..induced binding of ERalpha to both an AF-1 coactivator complex (p68/p72 and p300) and corepressor complexes (N-CoR/SMRT), BBP selectively enhanced the binding to the AF-1 coactivators...
  23. Park E, Schroen D, Yang M, Li H, Li L, Chen J. SMRTe, a silencing mediator for retinoid and thyroid hormone receptors-extended isoform that is more related to the nuclear receptor corepressor. Proc Natl Acad Sci U S A. 1999;96:3519-24 pubmed
    SMRT (silencing mediator for retinoid and thyroid hormone receptors) and N-CoR (nuclear receptor copressor) mediate transcriptional repression of important regulators that are involved in many signaling pathways...
  24. Oberoi J, Fairall L, Watson P, Yang J, Czimmerer Z, Kampmann T, et al. Structural basis for the assembly of the SMRT/NCoR core transcriptional repression machinery. Nat Struct Mol Biol. 2011;18:177-84 pubmed publisher
    ..Transcriptional repression complexes, assembled by the corepressor NCoR and its homolog SMRT, are crucial in many processes, including development and metabolic physiology...
  25. Fleming F, Hill A, McDermott E, O Higgins N, Young L. Differential recruitment of coregulator proteins steroid receptor coactivator-1 and silencing mediator for retinoid and thyroid receptors to the estrogen receptor-estrogen response element by beta-estradiol and 4-hydroxytamoxifen in human breast canc. J Clin Endocrinol Metab. 2004;89:375-83 pubmed
    ..4- Hydroxy-tamoxifen (4-OHT) increased ER-alpha and silencing mediator for retinoid and thyroid receptors (SMRT) expression and increased ER-ERE binding...
  26. Kao H, Downes M, Ordentlich P, Evans R. Isolation of a novel histone deacetylase reveals that class I and class II deacetylases promote SMRT-mediated repression. Genes Dev. 2000;14:55-66 pubmed
    The transcriptional corepressor SMRT functions by mediating the repressive effect of transcription factors involved in diverse signaling pathways...
  27. Farboud B, Hauksdottir H, Wu Y, Privalsky M. Isotype-restricted corepressor recruitment: a constitutively closed helix 12 conformation in retinoic acid receptors beta and gamma interferes with corepressor recruitment and prevents transcriptional repression. Mol Cell Biol. 2003;23:2844-58 pubmed
    ..Transcriptional repression is mediated through the recruitment of corepressor proteins such as SMRT. Notably, vertebrates encode three major forms of RARs, alpha, beta, and gamma, and these distinct RAR isotypes ..
  28. Moretti C, Serrentino M, Ialy Radio C, Delessard M, Soboleva T, Tores F, et al. SLY regulates genes involved in chromatin remodeling and interacts with TBL1XR1 during sperm differentiation. Cell Death Differ. 2017;24:1029-1044 pubmed publisher
    ..Altogether our results show that SLY is a regulator of sperm chromatin remodeling. Finally we identified that SMRT/N-CoR repressor complex is involved in gene regulation during sperm differentiation since members of this complex, ..
  29. Kiss M, Czimmerer Z, Nagy G, Bieniasz Krzywiec P, Ehling M, Pap A, et al. Retinoid X receptor suppresses a metastasis-promoting transcriptional program in myeloid cells via a ligand-insensitive mechanism. Proc Natl Acad Sci U S A. 2017;114:10725-10730 pubmed publisher
    ..with nuclear receptor corepressor (NCoR) and silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) corepressors and is largely unresponsive to ligand activation...
  30. Wang H, Jiang Z, Zhang J, Xie Z, Wang Y, Yang G. Enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells on titanium substrates by inhibiting Notch3. Arch Oral Biol. 2017;80:34-40 pubmed publisher
    ..Inhibition Notch3 can enhance osteogenic differentiation of BMSCs on SLA-treated Ti disks, which potentially provides a gene target for improving osseointegration. ..
  31. Rajendran R, Nye A, Frasor J, Balsara R, Martini P, Katzenellenbogen B. Regulation of nuclear receptor transcriptional activity by a novel DEAD box RNA helicase (DP97). J Biol Chem. 2003;278:4628-38 pubmed
    ..to a small region (amino acids 589-631) that has homology to a repression domain in the corepressor protein NCoR2/SMRTe. This region of DP97 is necessary and sufficient for its intrinsic repression activity...
  32. Nomura T, Khan M, Kaul S, Dong H, Wadhwa R, Colmenares C, et al. Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor. Genes Dev. 1999;13:412-23 pubmed
    The N-CoR/SMRT complex containing mSin3 and histone deacetylase (HDAC) mediates transcriptional repression by nuclear hormone receptors and Mad...
  33. Wagner S, Lupolova N, Gally D, Argyle S. Convergence of plasmid architectures drives emergence of multi-drug resistance in a clonally diverse Escherichia coli population from a veterinary clinical care setting. Vet Microbiol. 2017;211:6-14 pubmed publisher
    ..Illumina and single-molecule real-time (SMRT) sequencing were applied to assemble the complete genomes of E...
  34. Gelernter J, Sherva R, Koesterer R, Almasy L, Zhao H, Kranzler H, et al. Genome-wide association study of cocaine dependence and related traits: FAM53B identified as a risk gene. Mol Psychiatry. 2014;19:717-23 pubmed publisher
    ..NCOR2 (nuclear receptor corepressor 2) SNP rs150954431 was associated with P=1.19 × 10(-9) in the EA discovery sample...
  35. Hudson G, Watson P, Fairall L, Jamieson A, Schwabe J. Insights into the Recruitment of Class IIa Histone Deacetylases (HDACs) to the SMRT/NCoR Transcriptional Repression Complex. J Biol Chem. 2015;290:18237-44 pubmed publisher
    ..The class IIa HDACs are associated with the SMRT/NCoR repression complexes and this may, at least in part, account for their repressive activity...
  36. Zhao H, Li C, Pardo J, Chu P, Liao C, Huang J, et al. A novel E3 ubiquitin ligase TRAC-1 positively regulates T cell activation. J Immunol. 2005;174:5288-97 pubmed
    b>TRAC-1 (T cell RING (really interesting new gene) protein identified in activation screen) is a novel E3 ubiquitin ligase identified from a retroviral vector-based T cell surface activation marker screen...
  37. Szapary D, Song L, He Y, Simons S. Differential modulation of glucocorticoid and progesterone receptor transactivation. Mol Cell Endocrinol. 2008;283:114-26 pubmed publisher
    ..e., the corepressors NCoR and SMRT) modulate the EC50 and partial agonist activity for GR and PR induction of the same gene in opposite directions...
  38. Navaratnarajah P, Steele B, Redinbo M, Thompson N. Rifampicin-independent interactions between the pregnane X receptor ligand binding domain and peptide fragments of coactivator and corepressor proteins. Biochemistry. 2012;51:19-31 pubmed publisher
    ..PXR and a peptide fragment of the corepressor protein, the silencing mediator for retinoid and thyroid receptors (SMRT). An equilibrium dissociation constant of ~70 ?M was obtained for SMRT in the presence and absence of rifampicin...
  39. Meyer M, Pike J. Corepressors (NCoR and SMRT) as well as coactivators are recruited to positively regulated 1?,25-dihydroxyvitamin D3-responsive genes. J Steroid Biochem Mol Biol. 2013;136:120-4 pubmed publisher
    ..However, we also found that corepressor molecules such as NCoR and SMRT were present along with SRC1, CBP or MED1 at these 1,25(OH)2D3 activated gene enhancers...
  40. Snyder C, Goodson M, Schroeder A, Privalsky M. Regulation of corepressor alternative mRNA splicing by hormonal and metabolic signaling. Mol Cell Endocrinol. 2015;413:228-35 pubmed publisher
    Alternative mRNA splicing diversifies the products encoded by the NCoR and SMRT corepressor loci...
  41. Höpken U. Targeting HDAC3 in CREBBP-Mutant Lymphomas Counterstrikes Unopposed Enhancer Deacetylation of B-cell Signaling and Immune Response Genes. Cancer Discov. 2017;7:14-16 pubmed publisher
    ..colleagues addresses how somatic mutations in CREBBP disable acetylation and cause unopposed deacetylation by BCL6/SMRT/HDAC3 complexes on enhancers of B-cell signaling and immune response genes...
  42. Shi Y, Hon M, Evans R. The peroxisome proliferator-activated receptor delta, an integrator of transcriptional repression and nuclear receptor signaling. Proc Natl Acad Sci U S A. 2002;99:2613-8 pubmed
    ..response element and the association of nonliganded PPARdelta with corepressors SMRT (silencing mediator for retinoid and thyroid hormone receptors), SHARP (SMRT and histone deacetylase-associated repressor protein), and class I ..
  43. Melnick A, Carlile G, Ahmad K, Kiang C, Corcoran C, Bardwell V, et al. Critical residues within the BTB domain of PLZF and Bcl-6 modulate interaction with corepressors. Mol Cell Biol. 2002;22:1804-18 pubmed
    ..yet abrogated the ability of PLZF to repress transcription and led to the loss of interaction with N-CoR, SMRT, and histone deacetylases (HDACs)...
  44. Webb P, Valentine C, Nguyen P, Price R, Marimuthu A, West B, et al. ERbeta Binds N-CoR in the Presence of Estrogens via an LXXLL-like Motif in the N-CoR C-terminus. Nucl Recept. 2003;1:4 pubmed
    Nuclear receptors (NRs) usually bind the corepressors N-CoR and SMRT in the absence of ligand or in the presence of antagonists. Agonist binding leads to corepressor release and recruitment of coactivators...
  45. Hatzi K, Jiang Y, Huang C, Garrett Bakelman F, Gearhart M, Giannopoulou E, et al. A hybrid mechanism of action for BCL6 in B cells defined by formation of functionally distinct complexes at enhancers and promoters. Cell Rep. 2013;4:578-88 pubmed publisher
    ..These are (1) the formation of a unique ternary BCOR-SMRT complex at promoters, with each corepressor binding to symmetrical sites on BCL6 homodimers linked to specific ..
  46. Lee G, Elwood F, McNally J, Weiszmann J, Lindstrom M, Amaral K, et al. T0070907, a selective ligand for peroxisome proliferator-activated receptor gamma, functions as an antagonist of biochemical and cellular activities. J Biol Chem. 2002;277:19649-57 pubmed
    ..These results suggest that the activity of PPARgamma antagonists can be modulated by the availability and concentration of RXR agonists. T0070907 is a novel tool for the study of PPARgamma/RXRalpha heterodimer function. ..
  47. Xie L, Pi X, Townley Tilson W, Li N, Wehrens X, Entman M, et al. PHD2/3-dependent hydroxylation tunes cardiac response to β-adrenergic stress via phospholamban. J Clin Invest. 2015;125:2759-71 pubmed publisher
    ..Inhibition of PHDs increased the interaction between TR-α and nuclear receptor corepressor 2 (NCOR2) and suppressed Pln transcription...
  48. Bergfors A, Leenheer D, Bergqvist A, Ameur A, Lennerstrand J. Analysis of hepatitis C NS5A resistance associated polymorphisms using ultra deep single molecule real time (SMRT) sequencing. Antiviral Res. 2016;126:81-9 pubmed publisher
    ..Ultra-deep single molecule real time (SMRT) sequencing with the Pacific Biosciences (PacBio) RSII instrument was used to detect these RAVs...
  49. Huynh K, Fischle W, Verdin E, Bardwell V. BCoR, a novel corepressor involved in BCL-6 repression. Genes Dev. 2000;14:1810-23 pubmed
    ..The POZ domains of BCL-6 and several other POZ proteins interact with corepressors N-CoR and SMRT. Here we identify and characterize a novel corepressor BCoR (BCL-6 interacting corepressor), which is expressed ..
  50. Sekizuka T, Kawanishi M, Ohnishi M, Shima A, Kato K, Yamashita A, et al. Elucidation of quantitative structural diversity of remarkable rearrangement regions, shufflons, in IncI2 plasmids. Sci Rep. 2017;7:928 pubmed publisher
    ..those of IncI2 plasmids carrying mcr-1, of three Escherichia coli strains using single-molecule, real-time (SMRT) sequencing and Illumina sequencing...
  51. Bao E, Song C, Lan L. ReMILO: reference assisted misassembly detection algorithm using short and long reads. Bioinformatics. 2017;: pubmed publisher
    ..we introduce ReMILO, a reference assisted misassembly detection algorithm that uses both short reads and PacBio SMRT long reads...
  52. Li H, Leo C, Schroen D, Chen J. Characterization of receptor interaction and transcriptional repression by the corepressor SMRT. Mol Endocrinol. 1997;11:2025-37 pubmed
    b>SMRT (silencing mediator of retinoic acid and thyroid hormone receptor) and N-CoR (nuclear receptor corepressor) are two related transcriptional corepressors that contain separable domains capable of interacting with unliganded nuclear ..
  53. Conte M, Kocher T. An improved genome reference for the African cichlid, Metriaclima zebra. BMC Genomics. 2015;16:724 pubmed publisher
    ..the draft genome assembly of the African cichlid fish, Metriaclima zebra, using a set of Pacific Biosciences SMRT sequencing reads corresponding to 16.5× coverage of the genome...
  54. Lamba V, Panetta J, Strom S, Schuetz E. Genetic predictors of interindividual variability in hepatic CYP3A4 expression. J Pharmacol Exp Ther. 2010;332:1088-99 pubmed publisher
    ..We resequenced hepatic nuclear factor-3-beta (HNF3beta, FoxA2), HNF4alpha, HNF3gamma (FoxA3), nuclear receptor corepressor 2 (NCoR2), and regions of the CYP3A4 promoter and genotyped informative single-nucleotide polymorphisms ..
  55. Nakajima H, Brindle P, Handa M, Ihle J. Functional interaction of STAT5 and nuclear receptor co-repressor SMRT: implications in negative regulation of STAT5-dependent transcription. EMBO J. 2001;20:6836-44 pubmed
    ..for STAT5-interacting proteins by yeast two-hybrid screening, we identified the nuclear receptor co-repressor SMRT (silencing mediator for retinoic acid receptor and thyroid hormone receptor) as a potential STAT5-binding partner...
  56. Guo L, Chen C, Liang Q, Karim M, Gorska M, Alam R. Nuclear translocation of MEK1 triggers a complex T cell response through the corepressor silencing mediator of retinoid and thyroid hormone receptor. J Immunol. 2013;190:159-67 pubmed publisher
    ..MEK1 interacts with the nuclear receptor corepressor silencing mediator of retinoid and thyroid hormone receptor (SMRT). MEK1 reduces the nuclear level of SMRT in an activation-dependent manner...
  57. Leung K, Siu G, Tam K, To S, Rajwani R, Ho P, et al. Comparative Genomic Analysis of Two Clonally Related Multidrug Resistant Mycobacterium tuberculosis by Single Molecule Real Time Sequencing. Front Cell Infect Microbiol. 2017;7:478 pubmed publisher
    ..To better understand the genomic characteristics of MDR-TB, Single Molecule Real-Time (SMRT) Sequencing and comparative genomic analyses was performed to identify mutations that contributed to the stepwise ..
  58. Sander J, Schmidt S, Cirovic B, McGovern N, Papantonopoulou O, Hardt A, et al. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. Immunity. 2017;47:1051-1066.e12 pubmed publisher
    ..Finally, moDCs are phenotypically heterogeneous and therefore necessitate the use of high-dimensional phenotyping to open new possibilities for better clinical tailoring of these cellular therapies. ..
  59. Zhou R, Li X, Hu G, Gong A, Drescher K, Chen X. miR-16 targets transcriptional corepressor SMRT and modulates NF-kappaB-regulated transactivation of interleukin-8 gene. PLoS ONE. 2012;7:e30772 pubmed publisher
    ..of the IL-8 gene by suppression of the silencing mediator for retinoid and thyroid hormone receptor (SMRT)...
  60. Schulz M, Eggert M, Baniahmad A, Dostert A, Heinzel T, Renkawitz R. RU486-induced glucocorticoid receptor agonism is controlled by the receptor N terminus and by corepressor binding. J Biol Chem. 2002;277:26238-43 pubmed
    ..Notably, the N and the C termini of GR are both involved in corepressor binding. Thus, the N terminus of GR is a major determinant for RU486-dependent NCoR interaction as well as for RU486-mediated agonist activity. ..
  61. Ki S, Cho I, Choi D, Kim S. Glucocorticoid receptor (GR)-associated SMRT binding to C/EBPbeta TAD and Nrf2 Neh4/5: role of SMRT recruited to GR in GSTA2 gene repression. Mol Cell Biol. 2005;25:4150-65 pubmed
    ..chromatin immunoprecipitation, and GST pull-down assays revealed that silencing mediator for retinoid and thyroid hormone receptors (SMRT), a corepressor recruited to steroid-GR complex for histone deacetylation, bound ..
  62. Stengel K, Barnett K, Wang J, Liu Q, Hodges E, Hiebert S, et al. Deacetylase activity of histone deacetylase 3 is required for productive VDJ recombination and B-cell development. Proc Natl Acad Sci U S A. 2017;114:8608-8613 pubmed publisher
    Histone deacetylase 3 (HDAC3) is the catalytic component of NCoR/SMRT corepressor complexes that mediate the actions of transcription factors implicated in the regulation of B-cell development and function...
  63. Johnson D, Li C, Chen L, Ghosh J, Chen J. Regulation and binding of pregnane X receptor by nuclear receptor corepressor silencing mediator of retinoid and thyroid hormone receptors (SMRT). Mol Pharmacol. 2006;69:99-108 pubmed
    ..study, we analyzed interactions between PXR and the silencing mediator of retinoid and thyroid hormone receptors (SMRT) and determined the role of SMRT in regulating PXR activity...
  64. Song L, Gelmann E. Silencing mediator for retinoid and thyroid hormone receptor and nuclear receptor corepressor attenuate transcriptional activation by the beta-catenin-TCF4 complex. J Biol Chem. 2008;283:25988-99 pubmed publisher
    ..We observed that the proteins silencing mediator for retinoid and thyroid hormone receptor (SMRT) and the nuclear receptor corepressor (NCoR) are negative regulators of transcription induced by the beta-catenin-..
  65. Larkin J, Henley R, Jadhav V, Korlach J, Wanunu M. Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing. Nat Nanotechnol. 2017;12:1169-1175 pubmed publisher
    Compared with conventional methods, single-molecule real-time (SMRT) DNA sequencing exhibits longer read lengths than conventional methods, less GC bias, and the ability to read DNA base modifications...
  66. Eriksson L, Lif Holgerson P, Johansson I. Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community. Sci Rep. 2017;7:5861 pubmed publisher
    ..from 17-year-olds and mock bacteria communities were analysed using 16S rDNA Illumina MiSeq (v3-v4) and PacBio SMRT (v1-v8) sequencing including validity and reliability estimates. Caries was scored at 17 and 19 years of age...
  67. Grozinger C, Schreiber S. Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization. Proc Natl Acad Sci U S A. 2000;97:7835-40 pubmed
    ..Regulation of the cellular localization of HDAC4 and HDAC5 by 14-3-3 represents a mechanism for controlling the transcriptional activity of these class II HDAC proteins. ..
  68. Blackford J, Guo C, Zhu R, Dougherty E, Chow C, Simons S. Identification of location and kinetically defined mechanism of cofactors and reporter genes in the cascade of steroid-regulated transactivation. J Biol Chem. 2012;287:40982-95 pubmed publisher
    ..Current data suggest that how and where GR and the short form of SMRT act is also constant...
  69. Picard F, Kurtev M, Chung N, Topark Ngarm A, Senawong T, Machado De Oliveira R, et al. Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-gamma. Nature. 2004;429:771-6 pubmed
    ..Sirt1 represses PPAR-gamma by docking with its cofactors NCoR (nuclear receptor co-repressor) and SMRT (silencing mediator of retinoid and thyroid hormone receptors)...
  70. Parajuli P, Adamski M, Verma N. Bacteriophages are the major drivers of Shigella flexneri serotype 1c genome plasticity: a complete genome analysis. BMC Genomics. 2017;18:722 pubmed publisher
    ..flexneri serotype 1c using the hybrid methods of long-read single-molecule real-time (SMRT) sequencing technology and short-read MiSeq (Illumina) sequencing technology. The Y394 chromosome is 4...
  71. Wagner B, Valledor A, Shao G, Daige C, Bischoff E, Petrowski M, et al. Promoter-specific roles for liver X receptor/corepressor complexes in the regulation of ABCA1 and SREBP1 gene expression. Mol Cell Biol. 2003;23:5780-9 pubmed
    ..the nuclear receptor corepressor (NCoR) and the silencing mediator of retinoic acid and thyroid hormone receptors (SMRT)...
  72. Fallone F, Villard P, Seree E, Rimet O, Nguyen Q, Bourgarel Rey V, et al. Retinoids repress Ah receptor CYP1A1 induction pathway through the SMRT corepressor. Biochem Biophys Res Commun. 2004;322:551-6 pubmed
    ..Moreover, a physical interaction between AhR and the RAR-corepressor SMRT has been described in vitro...
  73. Woodhouse J, Makower A, Grossart H, Dittmann E. Draft Genome Sequences of Two Uncultured Armatimonadetes Associated with a Microcystis sp. (Cyanobacteria) Isolate. Genome Announc. 2017;5: pubmed publisher
    ..Here, two additional Armatimonadetes genome sequences were obtained via single-molecule real-time (SMRT) sequencing of an enrichment culture of the bloom-forming cyanobacterium Microcystis sp...
  74. Fu X, Li J, Tian Y, Quan W, Zhang S, Liu Q, et al. Long-read sequence assembly of the firefly Pyrocoelia pectoralis genome. Gigascience. 2017;: pubmed publisher
    ..Oliv., 1883) (Coleoptera: Lampyridae) using single molecule real time (SMRT) sequencing on the PacBio Sequel platform. In total, 57.8Gb long reads were generated and assembled into a 760...
  75. Zhou S, Fujimuro M, Hsieh J, Chen L, Miyamoto A, Weinmaster G, et al. SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function. Mol Cell Biol. 2000;20:2400-10 pubmed
    ..CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex...
  76. Hsu K, Kao H. ?-Transducin repeat-containing protein 1 (?-TrCP1)-mediated silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) protein degradation promotes tumor necrosis factor ? (TNF?)-induced inflammatory gene expression. J Biol Chem. 2013;288:25375-86 pubmed publisher
    ..In this report, we demonstrate that silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) represses the expression of a subset of TNF? target genes in human umbilical vein endothelial cells...
  77. Liu Y, Takeshita A, Misiti S, Chin W, Yen P. Lack of coactivator interaction can be a mechanism for dominant negative activity by mutant thyroid hormone receptors. Endocrinology. 1998;139:4197-204 pubmed
  78. Tagami T, Yamamoto H, Moriyama K, Sawai K, Usui T, Shimatsu A, et al. The retinoid X receptor binding to the thyroid hormone receptor: relationship with cofactor binding and transcriptional activity. J Mol Endocrinol. 2009;42:415-28 pubmed publisher
  79. Christel S, Herold M, Bellenberg S, El Hajjami M, Buetti Dinh A, Pivkin I, et al. Multi-omics reveal the lifestyle of the acidophilic, mineral-oxidizing model species Leptospirillum ferriphilumT. Appl Environ Microbiol. 2017;: pubmed publisher
    ..presents a complete, circular genome of Leptospirillum ferriphilumT DSM 14647 obtained by PacBio SMRT long read sequencing for use as a high quality reference...
  80. Miles R, Crockett D, Lim M, Elenitoba Johnson K. Analysis of BCL6-interacting proteins by tandem mass spectrometry. Mol Cell Proteomics. 2005;4:1898-909 pubmed
    ..Many of the novel proteins identified in this study suggest additional functional roles for BCL6 beyond transcriptional repression. ..