DROSHA

Summary

Gene Symbol: DROSHA
Description: drosha ribonuclease III
Alias: ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN, ribonuclease 3, RNase III, drosha, double-stranded RNA-specific endoribonuclease, nuclear RNase III Drosha, p241, ribonuclease type III, nuclear
Species: human
Products:     DROSHA

Top Publications

  1. Merritt W, Lin Y, Han L, Kamat A, Spannuth W, Schmandt R, et al. Dicer, Drosha, and outcomes in patients with ovarian cancer. N Engl J Med. 2008;359:2641-50 pubmed publisher
    We studied Dicer and Drosha, components of the RNA-interference machinery, in ovarian cancer...
  2. Lee Y, Ahn C, Han J, Choi H, Kim J, Yim J, et al. The nuclear RNase III Drosha initiates microRNA processing. Nature. 2003;425:415-9 pubmed
    ..Dicer, a member of the RNase III superfamily of bidentate nucleases, mediates the latter step, whereas the processing enzyme for the former step ..
  3. Shiohama A, Sasaki T, Noda S, Minoshima S, Shimizu N. Nucleolar localization of DGCR8 and identification of eleven DGCR8-associated proteins. Exp Cell Res. 2007;313:4196-207 pubmed
    ..We detected at least four kinds of protein complex, such as DROSHA/DGCR8, DGCR8/Nucleolin, DGCR8/ILF3 and ILF3/XPO5, by co-immunoprecipitation...
  4. Qin Y, Xia Y, Wu W, Han X, Lu C, Ji G, et al. Genetic variants in microRNA biogenesis pathway genes are associated with semen quality in a Han-Chinese population. Reprod Biomed Online. 2012;24:454-61 pubmed publisher
    ..Seven single-nucleotide polymorphisms (SNP) of DICER1 (rs13078, rs1057035 and rs12323635) and DROSHA (rs10719, rs2291109, rs17409893 and rs642321) were determined by TaqMan probes and SNPstream in 667 eligible ..
  5. Landthaler M, Yalcin A, Tuschl T. The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. Curr Biol. 2004;14:2162-7 pubmed
    ..Drosha RNase III catalyzes the first excision event, the release in the nucleus of a hairpin RNA (pre-miRNA), which is followed ..
  6. Wu H, Xu H, Miraglia L, Crooke S. Human RNase III is a 160-kDa protein involved in preribosomal RNA processing. J Biol Chem. 2000;275:36957-65 pubmed
    A human RNase III gene encodes a protein of 160 kDa with multiple domains, a proline-rich, a serine- and arginine-rich, and an RNase III domain...
  7. Davis B, Hilyard A, Lagna G, Hata A. SMAD proteins control DROSHA-mediated microRNA maturation. Nature. 2008;454:56-61 pubmed publisher
    ..promoting the processing of primary transcripts of miR-21 (pri-miR-21) into precursor miR-21 (pre-miR-21) by the DROSHA (also known as RNASEN) complex...
  8. Kim Y, Kim V. Processing of intronic microRNAs. EMBO J. 2007;26:775-83 pubmed
    ..The primary transcripts are cleaved by Drosha to release approximately 70 nt pre-miRNAs that are subsequently processed by Dicer to generate mature ..
  9. Shenoy A, Blelloch R. Genomic analysis suggests that mRNA destabilization by the microprocessor is specialized for the auto-regulation of Dgcr8. PLoS ONE. 2009;4:e6971 pubmed publisher
    The Microprocessor, containing the RNA binding protein Dgcr8 and RNase III enzyme Drosha, is responsible for processing primary microRNAs to precursor microRNAs...

More Information

Publications89

  1. Han J, Lee Y, Yeom K, Kim Y, Jin H, Kim V. The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev. 2004;18:3016-27 pubmed
    RNase III proteins play key roles in microRNA (miRNA) biogenesis. The nuclear RNase III Drosha cleaves primary miRNAs (pri-miRNAs) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic RNase III Dicer to ..
  2. Suzuki H, Yamagata K, Sugimoto K, Iwamoto T, Kato S, Miyazono K. Modulation of microRNA processing by p53. Nature. 2009;460:529-33 pubmed publisher
    ..In HCT116 cells and human diploid fibroblasts, p53 interacts with the Drosha processing complex through the association with DEAD-box RNA helicase p68 (also known as DDX5) and facilitates the ..
  3. Nakamura T, Canaani E, Croce C. Oncogenic All1 fusion proteins target Drosha-mediated microRNA processing. Proc Natl Acad Sci U S A. 2007;104:10980-5 pubmed
    ..and continuing into excision of a stem-loop hairpin structure within pri-miRNA by the nuclear RNaseIII enzyme Drosha, transportation to the cytoplasm, and further processing by a second RNaseIII enzyme Dicer, into a 22-base mature ..
  4. Gregory R, Yan K, Amuthan G, Chendrimada T, Doratotaj B, Cooch N, et al. The Microprocessor complex mediates the genesis of microRNAs. Nature. 2004;432:235-40 pubmed
    ..miRNAs are processed by the RNA-mediated interference machinery. Drosha is an RNase III enzyme that was recently implicated in miRNA processing...
  5. Scotto L, Narayan G, Nandula S, Subramaniyam S, Kaufmann A, Wright J, et al. Integrative genomics analysis of chromosome 5p gain in cervical cancer reveals target over-expressed genes, including Drosha. Mol Cancer. 2008;7:58 pubmed publisher
    ..One of the candidates identified was Drosha (RNASEN), a gene that is required in the first step of microRNA (miRNA) processing in the nucleus...
  6. Torres A, Torres K, Paszkowski T, Jodłowska Jedrych B, Radomański T, Ksiazek A, et al. Major regulators of microRNAs biogenesis Dicer and Drosha are down-regulated in endometrial cancer. Tumour Biol. 2011;32:769-76 pubmed publisher
    Alterations in microRNAs expression have been proposed to play role in endometrial cancer pathogenesis. Dicer and Drosha are main regulators of microRNA biogenesis and deregulation of their expression has been indicated as a possible ..
  7. Horikawa Y, Wood C, Yang H, Zhao H, Ye Y, Gu J, et al. Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma. Clin Cancer Res. 2008;14:7956-62 pubmed publisher
    ..We genotyped 40 single nucleotide polymorphisms (SNP) from 11 miRNA processing genes (DROSHA, DGCR8, XPO5, RAN, DICER1, TARBP2, AGO1, AGO2, GEMIN3, GEMIN4, HIWI) and 15 miRNA genes in 279 Caucasian patients ..
  8. Han J, Pedersen J, Kwon S, BELAIR C, Kim Y, Yeom K, et al. Posttranscriptional crossregulation between Drosha and DGCR8. Cell. 2009;136:75-84 pubmed publisher
    The Drosha-DGCR8 complex, also known as Microprocessor, is essential for microRNA (miRNA) maturation. Drosha functions as the catalytic subunit, while DGCR8 (also known as Pasha) recognizes the RNA substrate...
  9. Macias S, Plass M, Stajuda A, Michlewski G, Eyras E, Cáceres J. DGCR8 HITS-CLIP reveals novel functions for the Microprocessor. Nat Struct Mol Biol. 2012;19:760-6 pubmed publisher
    The Drosha-DGCR8 complex (Microprocessor) is required for microRNA (miRNA) biogenesis. DGCR8 recognizes the RNA substrate, whereas Drosha functions as the endonuclease...
  10. Kim J, Choi Y, Jin G, Kang H, Choi J, Jeon H, et al. Association of a common AGO1 variant with lung cancer risk: a two-stage case-control study. Mol Carcinog. 2010;49:913-21 pubmed publisher
    ..In stage 1 of the study, 24 SNPs in the 11 miRNA biosynthesis genes (DROSHA, DGCR8, RAN, XPO5, DICER, AGO1, AGO2, HIWI, GEMIN3, GEMIN4, and TRBP) were genotyped in 100 lung cancer patients ..
  11. Sugito N, Ishiguro H, Kuwabara Y, Kimura M, Mitsui A, Kurehara H, et al. RNASEN regulates cell proliferation and affects survival in esophageal cancer patients. Clin Cancer Res. 2006;12:7322-8 pubmed
    ..We studied the expression level of the miRNA processing enzyme (DICER1, DGCR8, and RNASEN) in esophageal squamous cell carcinoma (ESCC)...
  12. Irvin Wilson C, Chaudhuri G. Alternative initiation and splicing in dicer gene expression in human breast cells. Breast Cancer Res. 2005;7:R563-9 pubmed
    ..Furthermore, alternative splicing of its exon 2 and 3 sequences of their pre-mRNA creates a more translationally competent mRNA in these cells. ..
  13. Torrezan G, Ferreira E, Nakahata A, Barros B, Castro M, Correa B, et al. Recurrent somatic mutation in DROSHA induces microRNA profile changes in Wilms tumour. Nat Commun. 2014;5:4039 pubmed publisher
    ..Here we identify DROSHA mutations in 12% of WT samples (26/222) using whole-exome sequencing and targeted sequencing of 10 microRNA (miRNA)..
  14. Lin Y, Sullivan C. Expanding the role of Drosha to the regulation of viral gene expression. Proc Natl Acad Sci U S A. 2011;108:11229-34 pubmed publisher
    ..Here, we show that Drosha, an endoribonuclease best known for its role in the biogenesis of microRNAs (miRNAs), can also function to ..
  15. Lin J, Horikawa Y, Tamboli P, Clague J, Wood C, Wu X. Genetic variations in microRNA-related genes are associated with survival and recurrence in patients with renal cell carcinoma. Carcinogenesis. 2010;31:1805-12 pubmed publisher
    ..Haplotypes of DICER and DROSHA were also associated with altered patient survival and recurrence...
  16. Yan M, Huang H, Wang T, Wan Y, Cui S, Liu Z, et al. Dysregulated expression of dicer and drosha in breast cancer. Pathol Oncol Res. 2012;18:343-8 pubmed publisher
    ..Altered expression of Dicer and Drosha, two key enzymes in the miRNA maturation, is believed to be one of the most important mechanisms...
  17. Davis B, Hilyard A, Nguyen P, Lagna G, Hata A. Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha. Mol Cell. 2010;39:373-84 pubmed publisher
    ..protein (BMP), the Smads, promote the expression of a subset of miRNAs by facilitating the cleavage reaction by Drosha. The mechanism that limits Smad-mediated processing to a selective group of miRNAs remained hitherto unexplored...
  18. Khoshnaw S, Rakha E, Abdel Fatah T, Nolan C, Hodi Z, Macmillan R, et al. The microRNA maturation regulator Drosha is an independent predictor of outcome in breast cancer patients. Breast Cancer Res Treat. 2013;137:139-53 pubmed publisher
    b>Drosha is a protein that plays a key role in the biogenesis of microRNAs which are well known to be deranged in human breast cancer (BC)...
  19. Gromak N, Dienstbier M, Macias S, Plass M, Eyras E, Cáceres J, et al. Drosha regulates gene expression independently of RNA cleavage function. Cell Rep. 2013;5:1499-510 pubmed publisher
    Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus...
  20. Filippov V, Solovyev V, Filippova M, Gill S. A novel type of RNase III family proteins in eukaryotes. Gene. 2000;245:213-21 pubmed
    The RNase III family of double-stranded RNA-specific endonucleases is characterized by the presence of a highly conserved 9 amino acid stretch in their catalytic center known as the RNase III signature motif...
  21. Zeng Y, Yi R, Cullen B. Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha. EMBO J. 2005;24:138-48 pubmed
    ..human microRNA maturation is the processing of the primary microRNA transcript by the nuclear RNaseIII enzyme Drosha to generate the approximately 60-nucleotide precursor microRNA hairpin...
  22. Han Y, Liu Y, Gui Y, Cai Z. Inducing cell proliferation inhibition and apoptosis via silencing Dicer, Drosha, and Exportin 5 in urothelial carcinoma of the bladder. J Surg Oncol. 2013;107:201-5 pubmed publisher
    MicroRNAs (miRNAs) are aberrantly expressed in cancers. Dicer, Drosha, and Exportin 5 are essential for miRNA processing...
  23. Lin R, Lin Y, Chen J, Kuo H, Chen Y, Diccianni M, et al. microRNA signature and expression of Dicer and Drosha can predict prognosis and delineate risk groups in neuroblastoma. Cancer Res. 2010;70:7841-50 pubmed publisher
    ..To investigate the microRNA (miRNA) profile and role of Dicer and Drosha in neuroblastoma, we assessed the expression of 162 human miRNAs, Dicer and Drosha in 66 neuroblastoma tumors by ..
  24. Yu B, Bi L, Zheng B, Ji L, Chevalier D, Agarwal M, et al. The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis. Proc Natl Acad Sci U S A. 2008;105:10073-8 pubmed publisher
    ..In addition, we found that SNIP1, the human homolog of DDL, is involved in miRNA biogenesis and interacts with Drosha. Therefore, we uncovered an evolutionarily conserved factor in miRNA biogenesis...
  25. Murai K, Sun G, Ye P, Tian E, Yang S, Cui Q, et al. The TLX-miR-219 cascade regulates neural stem cell proliferation in neurodevelopment and schizophrenia iPSC model. Nat Commun. 2016;7:10965 pubmed publisher
    ..Moreover, this study reveals an unexpected role for TLX in regulating microRNA processing, independent of its well-characterized role in transcriptional regulation. ..
  26. O Sullivan C, Christie J, Pienaar M, Gambling J, Nickerson P, Alford S, et al. Mutagenesis of ARS2 Domains To Assess Possible Roles in Cell Cycle Progression and MicroRNA and Replication-Dependent Histone mRNA Biogenesis. Mol Cell Biol. 2015;35:3753-67 pubmed publisher
    ..Mutations targeting the Mid domain implicated it in DROSHA interaction and microRNA biogenesis...
  27. Rostami Mogaddam M, Safavi Ardabili N, Shafaeei Y, Maleki N, Jafari N, Jafari A. Overexpression of Drosha, DiGeorge syndrome critical region gene 8 (DGCR8), and Dicer mRNAs in the pathogenesis of psoriasis. J Cosmet Dermatol. 2017;16:e48-e53 pubmed publisher
    ..Recent studies have indicated that microRNAs (miRNAs) play important roles in psoriasis...
  28. Hu S, Liu L, Chang E, Wang J, Raufman J. Butyrate inhibits pro-proliferative miR-92a by diminishing c-Myc-induced miR-17-92a cluster transcription in human colon cancer cells. Mol Cancer. 2015;14:180 pubmed publisher
    ..The effects of butyrate, SAHA and valproic acid on protein levels of c-Myc, Drosha and p57 were measured in HCT-116 cells using immunoblotting...
  29. Nepal C, Coolen M, Hadzhiev Y, Cussigh D, Mydel P, Steen V, et al. Transcriptional, post-transcriptional and chromatin-associated regulation of pri-miRNAs, pre-miRNAs and moRNAs. Nucleic Acids Res. 2016;44:3070-81 pubmed publisher
    ..Furthermore, we demonstrated that CAGE-seq also detects 3'-end processing of pre-miRNAs on Drosha cleavage site that correlates with miRNA-offset RNAs (moRNAs) production and provides a new tool for detecting ..
  30. Zhang H, Hou Y, Xu L, Zeng Z, Wen S, Du Y, et al. Cytoplasmic Drosha Is Aberrant in Precancerous Lesions of Gastric Carcinoma and Its Loss Predicts Worse Outcome for Gastric Cancer Patients. Dig Dis Sci. 2016;61:1080-90 pubmed publisher
    The nuclear localization of Drosha is critical for its function as a microRNA maturation regulator...
  31. Hessam S, Sand M, Skrygan M, Gambichler T, Bechara F. Inflammation induced changes in the expression levels of components of the microRNA maturation machinery Drosha, Dicer, Drosha co-factor DGRC8 and Exportin-5 in inflammatory lesions of hidradenitis suppurativa patients. J Dermatol Sci. 2016;82:166-74 pubmed publisher
    ..To evaluate expression of the miRNA key regulators Drosha, Drosha co-factor DGRC8, Dicer and Exportin-5 in the inflammatory microenvironment of HS...
  32. Zheng Q, Yang H, Yuan Y. Autoantigen La Regulates MicroRNA Processing from Stem-Loop Precursors by Association with DGCR8. Biochemistry. 2017;56:6098-6110 pubmed publisher
    ..primary microRNA (pri-miRNA) processing starts from precise cleavage of the stem loop, which is catalyzed by the Drosha-DGCR8 complex...
  33. Patne K, Rakesh R, Arya V, Chanana U, Sethy R, Swer P, et al. BRG1 and SMARCAL1 transcriptionally co-regulate DROSHA, DGCR8 and DICER in response to doxorubicin-induced DNA damage. Biochim Biophys Acta Gene Regul Mech. 2017;1860:936-951 pubmed publisher
    ..SMARCAL1, members of the ATP-dependent chromatin remodelling family, are shown to co-regulate the transcription of DROSHA, DGCR8, and DICER in response to double-strand DNA breaks induced by doxorubicin...
  34. Calses P, Dhillon K, Tucker N, Chi Y, Huang J, Kawasumi M, et al. DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing. Cell Rep. 2017;19:162-174 pubmed publisher
    ..Here, we demonstrate an unexpected role for DGCR8, an RNA binding protein that canonically functions with Drosha to mediate microRNA processing, in the repair of UV-induced DNA lesions...
  35. Rasschaert P, Figueroa T, Dambrine G, Rasschaert D, Laurent S. Alternative splicing of a viral mirtron differentially affects the expression of other microRNAs from its cluster and of the host transcript. RNA Biol. 2016;13:1310-1322 pubmed
    ..b>Drosha silencing and alternative splicing of an extended exon 2 of the LAT lncRNA from a newly identified 3' splice site (..
  36. Oskowitz A, Penfornis P, Tucker A, Prockop D, Pochampally R. Drosha regulates hMSCs cell cycle progression through a miRNA independent mechanism. Int J Biochem Cell Biol. 2011;43:1563-72 pubmed publisher
    ..To determine the role of the miRNA pathway in hMSCs proliferation, Drosha and Dicer knockdown hMSCs were generated using a lentiviral based tetracycline inducible shRNA...
  37. Liu Y, Zhao R, Wang H, Luo Y, Wang X, Niu W, et al. miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma. Cell Death Dis. 2016;7:e2156 pubmed publisher
    ..compared the control cells, while there was no obvious effect on the expression levels of the two critical enzymes Drosha and Dicer...
  38. Garibaldi F, Falcone E, Trisciuoglio D, Colombo T, Lisek K, Walerych D, et al. Mutant p53 inhibits miRNA biogenesis by interfering with the microprocessor complex. Oncogene. 2016;35:3760-70 pubmed publisher
    ..that endogenous mutp53 binds and sequesters RNA helicases p72/82 from the microprocessor complex, interfering with Drosha-pri-miRNAs association...
  39. Hansen T, Venø M, Jensen T, Schaefer A, Damgaard C, Kjems J. Argonaute-associated short introns are a novel class of gene regulators. Nat Commun. 2016;7:11538 pubmed publisher
    ..Mature miRNAs are produced by two sequential endonucleolytic cleavages facilitated by Drosha in the nucleus and Dicer in the cytoplasm...
  40. Gadd S, Huff V, Walz A, Ooms A, Armstrong A, Gerhard D, et al. A Children's Oncology Group and TARGET initiative exploring the genetic landscape of Wilms tumor. Nat Genet. 2017;49:1487-1494 pubmed publisher
    ..In addition to genes previously implicated in Wilms tumors (WT1, CTNNB1, AMER1, DROSHA, DGCR8, XPO5, DICER1, SIX1, SIX2, MLLT1, MYCN, and TP53), we identified mutations in genes not previously ..
  41. Liu H, Liang C, Kollipara R, Matsui M, Ke X, Jeong B, et al. HP1BP3, a Chromatin Retention Factor for Co-transcriptional MicroRNA Processing. Mol Cell. 2016;63:420-32 pubmed publisher
    Recent studies suggest that the microprocessor (Drosha-DGCR8) complex can be recruited to chromatin to catalyze co-transcriptional processing of primary microRNAs (pri-miRNAs) in mammalian cells...
  42. Zhang X, Gee H, Rose B, Lee C, Clark J, Elliott M, et al. Regulation of the tumour suppressor PDCD4 by miR-499 and miR-21 in oropharyngeal cancers. BMC Cancer. 2016;16:86 pubmed publisher
    ..Interestingly, expression of the miRNA machinery, Dicer1, Drosha, DDX5 (Dead Box Helicase 5) and DGCR8 (DiGeorge Syndrome Critical Region Gene 8) were all elevated by greater than ..
  43. Burke J, Kelenis D, Kincaid R, Sullivan C. A central role for the primary microRNA stem in guiding the position and efficiency of Drosha processing of a viral pri-miRNA. RNA. 2014;20:1068-77 pubmed publisher
    Processing of primary microRNA (pri-miRNA) stem-loops by the Drosha-DGCR8 complex is the initial step in miRNA maturation and crucial for miRNA function...
  44. Zhang H, Xu L, Zhou M, Du Y, Wen S, Liu M. [A negative correlation between Drosha expression and gastric adenocarcinoma malignancy and invasion]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015;31:1519-22, 1527 pubmed
    To investigate the role of Drosha expression in the progression of gastric adenocarcinoma and its impact on the invasive ability of SGC-7901 human gastric cancer cells...
  45. Anderson T, Glazebrook K, Murphy B, Viers L, Hieken T. Cross-sectional imaging to evaluate the extent of regional nodal disease in breast cancer patients undergoing neoadjuvant systemic therapy. Eur J Radiol. 2017;89:163-168 pubmed publisher
    ..Pre-treatment rN stage across all the patients was rN0 in 86 (25%), rN1 in 173 (50%), and rN3 in 89 (26%)...
  46. Burke J, Kuny C, Kincaid R, Sullivan C. Identification, validation, and characterization of noncanonical miRNAs. Methods. 2015;91:57-68 pubmed publisher
    ..While most miRNAs are generated through the activity of RNA Polymerase II (RNAP II) and subsequent processing by Drosha and Dicer, some viral miRNAs utilize alternative pathways of biogenesis...
  47. Jafari N, Shaghaghi H, Mahmoodi D, Shirzad Z, Alibeiki F, Bohlooli S, et al. Overexpression of microRNA biogenesis machinery: Drosha, DGCR8 and Dicer in multiple sclerosis patients. J Clin Neurosci. 2015;22:200-3 pubmed publisher
    We aimed to evaluate the expression of the major components of microRNA biogenesis machinery including Drosha, Dicer and DiGeorge syndrome critical region gene 8 (DGCR8) in multiple sclerosis (MS) patients...
  48. Nguyen T, Jo M, Choi Y, Park J, Kwon S, Hohng S, et al. Functional Anatomy of the Human Microprocessor. Cell. 2015;161:1374-87 pubmed publisher
    MicroRNA (miRNA) maturation is initiated by Microprocessor composed of RNase III DROSHA and its cofactor DGCR8, whose fidelity is critical for generation of functional miRNAs...
  49. Akpa M, Iglesias D, Chu L, Thiébaut A, Jentoft I, Hammond L, et al. Wilms Tumor Suppressor, WT1, Cooperates with MicroRNA-26a and MicroRNA-101 to Suppress Translation of the Polycomb Protein, EZH2, in Mesenchymal Stem Cells. J Biol Chem. 2016;291:3785-95 pubmed publisher
    ..of WT1; recently, it has been found that Wilms tumors may also be associated with biallelic mutations in DICER1 or DROSHA, crucial for miRNA biogenesis...
  50. Rupaimoole R, Ivan C, Yang D, Gharpure K, Wu S, Pecot C, et al. Hypoxia-upregulated microRNA-630 targets Dicer, leading to increased tumor progression. Oncogene. 2016;35:4312-20 pubmed publisher
    ..Recent studies have shown that hypoxia downregulates Drosha and Dicer, key enzymes in miRNA biogenesis, causing a decreased pool of miRNAs in cancer and resulting in ..
  51. Vincenzi B, Zoccoli A, Schiavon G, Iuliani M, Pantano F, Dell Aquila E, et al. Dicer and Drosha expression and response to Bevacizumab-based therapy in advanced colorectal cancer patients. Eur J Cancer. 2013;49:1501-8 pubmed publisher
    The miRNA-regulating enzymes Dicer and Drosha exhibit aberrant expression in several cancer types. Dicer and Drosha play a crucial role during the angiogenetic process in vitro and, for Dicer, in vivo...
  52. Yuan H, Deng R, Zhao X, Chen R, Hou G, Zhang H, et al. SUMO1 modification of KHSRP regulates tumorigenesis by preventing the TL-G-Rich miRNA biogenesis. Mol Cancer. 2017;16:157 pubmed publisher
    ..nucleic acid binding protein with roles in transcription and mRNA decay, and it is also a component of the Drosha-DGCR8 complex promoting the miRNA biogenesis...
  53. Poling B, Tsai K, Kang D, Ren L, Kennedy E, Cullen B. A lentiviral vector bearing a reverse intron demonstrates superior expression of both proteins and microRNAs. RNA Biol. 2017;14:1570-1579 pubmed publisher
    ..titer and the expression of any miRNA-linked indicator gene due to cleavage of the vector RNA genome by cellular Drosha. Here, we describe a novel lentiviral vector design in which transgenes and/or miRNAs are expressed using an ..
  54. Gelmez M, Coskunpinar E, Saracoglu B, Deniz G, Aktan M. Investigation of AID, Dicer, and Drosha Expressions in Patients with Chronic Lymphocytic Leukemia. Immunol Invest. 2017;46:433-446 pubmed publisher
    ..Dicer and Drosha, which have a role in activation process of miRNA, also act in a double-strand DNA break (DSB) repair mechanism...
  55. Xu L, Hou Y, Tu G, Chen Y, Du Y, Zhang H, et al. Nuclear Drosha enhances cell invasion via an EGFR-ERK1/2-MMP7 signaling pathway induced by dysregulated miRNA-622/197 and their targets LAMC2 and CD82 in gastric cancer. Cell Death Dis. 2017;8:e2642 pubmed publisher
    b>Drosha is an RNA III-like enzyme that has an aberrant expression in some tumors. Our previous studies showed the aberrant Drosha in gastric tumors...
  56. Cho S, Ko J, Kim J, Jeon Y, Yoo J, Oh J, et al. 3'-UTR Polymorphisms in the MiRNA Machinery Genes DROSHA, DICER1, RAN, and XPO5 Are Associated with Colorectal Cancer Risk in a Korean Population. PLoS ONE. 2015;10:e0131125 pubmed publisher
    ..CT heterozygotes and T allele carriers (CT + TT) genotypes had lower risk of CRC, while the DICER1 rs3742330, DROSHA rs10719, and XPO5 rs11077 polymorphisms were not associated with CRC in the full study sample...
  57. Poling B, Price A, Luftig M, Cullen B. The Epstein-Barr virus miR-BHRF1 microRNAs regulate viral gene expression in cis. Virology. 2017;512:113-123 pubmed publisher
    ..are present in the 3'UTR of transcripts encoding EBNA-LP and that excision of pre-miR-BHRF1-2 and 1-3 by Drosha destabilizes these mRNAs and reduces expression of the encoded protein...
  58. Daugaard I, Hansen T. Biogenesis and Function of Ago-Associated RNAs. Trends Genet. 2017;33:208-219 pubmed publisher
    ..best-described class of post-transcriptional regulators that follow a specific biogenesis pathway characterized by Drosha/DGCR8 and Dicer processing...
  59. Yuan L, Chu H, Wang M, Gu X, Shi D, Ma L, et al. Genetic variation in DROSHA 3'UTR regulated by hsa-miR-27b is associated with bladder cancer risk. PLoS ONE. 2013;8:e81524 pubmed publisher
    ..DICER and DROSHA are two members of RNase III family, playing pivotal roles in the pathway of miRNAs biogenesis...
  60. Porta S, Kwong L, Trojanowski J, Lee V. Drosha inclusions are new components of dipeptide-repeat protein aggregates in FTLD-TDP and ALS C9orf72 expansion cases. J Neuropathol Exp Neurol. 2015;74:380-7 pubmed publisher
    ..Here, we identify Drosha protein as a new component of these dipeptide-repeat aggregates...
  61. Jafarnejad S, Sjoestroem C, Martinka M, Li G. Expression of the RNase III enzyme DROSHA is reduced during progression of human cutaneous melanoma. Mod Pathol. 2013;26:902-10 pubmed publisher
    ..Here, we investigated the expression pattern of DROSHA in a large set of melanocytic lesions (n=409) by tissue microarray and immunohistochemistry...
  62. Licht K, Jantsch M. The Other Face of an Editor: ADAR1 Functions in Editing-Independent Ways. Bioessays. 2017;39: pubmed publisher
    ..ADAR1 is also known to recruit proteins such as DROSHA to nascent transcripts. Still, many open questions remain...
  63. Church V, Pressman S, Isaji M, Truscott M, Cizmecioglu N, Buratowski S, et al. Microprocessor Recruitment to Elongating RNA Polymerase II Is Required for Differential Expression of MicroRNAs. Cell Rep. 2017;20:3123-3134 pubmed publisher
    ..The Microprocessor complex of Drosha and DGCR8 carries this out, but it has been unclear what controls Microprocessor's differential processing of ..
  64. FORTIN K, Nicholson R, Nicholson A. Mouse ribonuclease III. cDNA structure, expression analysis, and chromosomal location. BMC Genomics. 2002;3:26 pubmed
    ..A human RNase III orthologue has been implicated in ribosomal RNA maturation...
  65. Beveridge N, Gardiner E, Carroll A, Tooney P, Cairns M. Schizophrenia is associated with an increase in cortical microRNA biogenesis. Mol Psychiatry. 2010;15:1176-89 pubmed publisher
    ..Given the magnitude of microRNA changes and their wide sphere of influence, this phenomenon could represent an important dimension in the pathogenesis of schizophrenia. ..
  66. Allegra D, Bilan V, Garding A, Dohner H, Stilgenbauer S, Kuchenbauer F, et al. Defective DROSHA processing contributes to downregulation of MiR-15/-16 in chronic lymphocytic leukemia. Leukemia. 2014;28:98-107 pubmed publisher
    ..CLL patients (22/38) compared with non-malignant B-cells (n=14), indicating a block of miRNA maturation at the DROSHA processing step...
  67. Chakraborty S, Krishnan Y. A structural map of oncomiR-1 at single-nucleotide resolution. Nucleic Acids Res. 2017;45:9694-9705 pubmed publisher
    ..accessible regions of oncomiR-1 reveal that most of its primary microRNA domains are suboptimal substrates for Drosha-DGCR8, and therefore resistant to microprocessing...
  68. Xing L, Kieff E. Epstein-Barr virus BHRF1 micro- and stable RNAs during latency III and after induction of replication. J Virol. 2007;81:9967-75 pubmed
    ..This 1.3-kb RNA is the residue of Drosha cleavage of the BHRF1 microRNAs from EBNA transcripts...
  69. Rouleau S, Garant J, Bolduc F, Bisaillon M, Perreault J. G-Quadruplexes influence pri-microRNA processing. RNA Biol. 2017;: pubmed publisher
    ..However, their impact on pri-miRNA processing remains unknown. We identified G4 located near the Drosha cleavage site in three distinct pri-miRNAs: pri-mir200c, pri-mir451a, and pri-mir497...
  70. Antoniali G, Serra F, Lirussi L, Tanaka M, D Ambrosio C, Zhang S, et al. Mammalian APE1 controls miRNA processing and its interactome is linked to cancer RNA metabolism. Nat Commun. 2017;8:797 pubmed publisher
    ..a role for apurinic/apyrimidinic endonuclease 1 in pri-miRNA processing and stability via association with the DROSHA-processing complex during genotoxic stress...
  71. Wegert J, Ishaque N, Vardapour R, Geörg C, Gu Z, Bieg M, et al. Mutations in the SIX1/2 pathway and the DROSHA/DGCR8 miRNA microprocessor complex underlie high-risk blastemal type Wilms tumors. Cancer Cell. 2015;27:298-311 pubmed publisher
    ..1% of blastemal cases); mutations in the DROSHA/DGCR8 microprocessor genes (18...
  72. Rahimi G, Jafari N, Khodabakhsh M, Shirzad Z, Dogaheh H. Upregulation of microRNA processing enzymes Drosha and Dicer in gestational diabetes mellitus. Gynecol Endocrinol. 2015;31:156-9 pubmed publisher
    ..Dicer and Drosha are two major enzymes in the miRNA biogenesis process...
  73. Papachristou D, Sklirou E, Corradi D, Grassani C, Kontogeorgakos V, Rao U. Immunohistochemical analysis of the endoribonucleases Drosha, Dicer and Ago2 in smooth muscle tumours of soft tissues. Histopathology. 2012;60:E28-36 pubmed publisher
    ..aims of the present study were to determine the protein levels and cellular distribution of the endoribonucleases Drosha, Dicer and Ago2, major components of the microRNA-processing machinery, in benign and malignant soft tissue smooth ..
  74. Lee D, Nam J, Shin C. DROSHA targets its own transcript to modulate alternative splicing. RNA. 2017;23:1035-1047 pubmed publisher
    The nuclear RNase III enzyme DROSHA interacts with its cofactor DGCR8 to form the Microprocessor complex, which initiates microRNA (miRNA) maturation by cleaving hairpin structures embedded in primary transcripts...
  75. Boni V, Zarate R, Villa J, Bandres E, Gomez M, Maiello E, et al. Role of primary miRNA polymorphic variants in metastatic colon cancer patients treated with 5-fluorouracil and irinotecan. Pharmacogenomics J. 2011;11:429-36 pubmed publisher
    ..Our results suggest that miRNA polymorphic variants might be useful predictors of clinical outcome in mCRC patients treated with 5-fluorouracil and CPT-11 combination. ..
  76. Santamaria C, Muntion S, Roson B, Blanco B, Lopez Villar O, Carrancio S, et al. Impaired expression of DICER, DROSHA, SBDS and some microRNAs in mesenchymal stromal cells from myelodysplastic syndrome patients. Haematologica. 2012;97:1218-24 pubmed publisher
    ..Our aim was to analyze DICER1 and DROSHA gene and protein expression in mesenchymal stromal cells (the osteoblastic progenitors) obtained from bone marrow ..
  77. Starega Roslan J, Witkos T, Galka Marciniak P, Krzyzosiak W. Sequence features of Drosha and Dicer cleavage sites affect the complexity of isomiRs. Int J Mol Sci. 2015;16:8110-27 pubmed publisher
    ..isomiRs are formed from single miRNA genes and that this effect is attributable mainly to imprecise cleavage by Drosha and Dicer...
  78. Borras C, Serna E, Gambini J, Ingles M, Vina J. Centenarians maintain miRNA biogenesis pathway while it is impaired in octogenarians. Mech Ageing Dev. 2017;168:54-57 pubmed publisher
    ..We have looked into miRNA biogenesis. We show that RNA POL II, DROSHA, EXPORTIN 5 and DICER, are up-regulated in centenarians compared with octogenarians...
  79. Guo X, Liao Q, Chen P, Li X, Xiong W, Ma J, et al. The microRNA-processing enzymes: Drosha and Dicer can predict prognosis of nasopharyngeal carcinoma. J Cancer Res Clin Oncol. 2012;138:49-56 pubmed publisher
    ..In this study, we investigated the expression profiles of the two most important enzymes of the miRNA machinery, Drosha and Dicer, which were closely correlated with nasopharyngeal carcinoma (NPC) and patient survival.
  80. Xu L, Chen Y, Wen S, Du Y, Tang X, Liu M. [Knockdown of Drosha promotes chemosensitivity of epirubicin for gastric cancer MGC-803 cells]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016;32:1207-11 pubmed
    Objective To establish a gastric cancer cell line with stable Drosha silenced and explore the effect of Drosha on the chemosensitivity of gastric cancer cells to epirubicin...