hslV

Summary

Gene Symbol: hslV
Description: ATP-dependent protease peptidase subunit
Species: Escherichia coli O157:H7 EDL933

Top Publications

  1. ncbi Identification of a conditionally essential heat shock protein in Escherichia coli
    L F Peruski
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620
    Biochim Biophys Acta 1207:165-72. 1994
  2. ncbi Effects of the cys mutations on structure and function of the ATP-dependent HslVU protease in Escherichia coli. The Cys287 to Val mutation in HslU uncouples the ATP-dependent proteolysis by HslvU from ATP hydrolysis
    S J Yoo
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 273:22929-35. 1998
  3. ncbi Crystal structure determination of Escherichia coli ClpP starting from an EM-derived mask
    J Wang
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520 8114, USA
    J Struct Biol 124:151-63. 1998
  4. pmc Redundant in vivo proteolytic activities of Escherichia coli Lon and the ClpYQ (HslUV) protease
    W F Wu
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892 4255, USA
    J Bacteriol 181:3681-7. 1999
  5. ncbi ATP-dependent degradation of SulA, a cell division inhibitor, by the HslVU protease in Escherichia coli
    I S Seong
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, South Korea
    FEBS Lett 456:211-4. 1999
  6. ncbi Effect of ELF magnetic fields on protein synthesis in Escherichia coli K12
    S Nakasono
    Bio Science Department, Abiko Research Laboratory, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko City, Chiba 270 1194, Japan
    Radiat Res 154:208-16. 2000
  7. pmc Mutational studies on HslU and its docking mode with HslV
    H K Song
    Abteilung Strukturforschung, Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Planegg Martinsried, Germany
    Proc Natl Acad Sci U S A 97:14103-8. 2000
  8. pmc The ATP-dependent CodWX (HslVU) protease in Bacillus subtilis is an N-terminal serine protease
    M S Kang
    School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    EMBO J 20:734-42. 2001
  9. ncbi Crystal structures of the HslVU peptidase-ATPase complex reveal an ATP-dependent proteolysis mechanism
    J Wang
    Department of Molecular Biophysics, Biochemistry, 266 Whitney Avenue, Yale University, 06520, New Haven, CT, USA
    Structure 9:177-84. 2001
  10. ncbi A corrected quaternary arrangement of the peptidase HslV and atpase HslU in a cocrystal structure
    J Wang
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, Connecticut 06520 8114, USA
    J Struct Biol 134:15-24. 2001

Scientific Experts

  • Jimin Wang
  • Chirstoph Gille
  • M M Khattar
  • S J Yoo
  • J H Seol
  • C H Chung
  • M S Kang
  • A L Goldberg
  • I S Seong
  • M Rohrwild
  • D H Shin
  • Monika Slominska
  • Yi Ying Lee
  • K Tanaka
  • Ihn Sik Seong
  • M Bochtler
  • Bernard Couvreur
  • M Lin
  • H K Song
  • Y K Shim
  • S Nakasono
  • W F Wu
  • R Huber
  • S Gottesman
  • H Huang
  • M Bogyo
  • H C Huang
  • Chien Hung Yu
  • Kirsten Skarstad
  • Grzegorz Wegrzyn
  • Meng Ching Chen
  • Anne Wahl
  • Chueh Ling Kuo
  • Chiung Fang Chang
  • Pei I Lin
  • Whi Fin Wu
  • Jung Wook Lee
  • Min Kyung Choi
  • Alex Bollen
  • Min Suk Kang
  • M Kessel
  • Paul Falmagne
  • J Becker
  • Rudy Wattiez
  • Ohn Jo Koh
  • Dominique Le Ray
  • D Missiakas
  • Chin Ha Chung
  • Jean Claude Dujardin
  • Soo Hyun Eom
  • Y Li
  • B K Lim
  • N Tanahashi
  • H Saiki
  • L Moroder
  • C Hartmann
  • R Behrendt
  • R Ramachandran
  • J Y Oh
  • Y Zhou
  • L F Peruski
  • H H Kim
  • C S Lee
  • N Shimbara
  • L Ditzel
  • D H Lee
  • T N Akopian
  • W Baumeister
  • S A Muller
  • U Santarius
  • J S McMaster
  • D Tortorella
  • G Pfeifer
  • H Ploegh
  • A Engel
  • M Groll
  • A F Kisselev
  • M Gaczynska
  • E Kocsis
  • S Raina
  • M Brendel
  • S E Chuang
  • R P Moerschell
  • A C Steven
  • J M Betton
  • C Georgopoulos
  • M R Maurizi
  • F Schwager
  • W Wu
  • O Coux

Detail Information

Publications34

  1. ncbi Identification of a conditionally essential heat shock protein in Escherichia coli
    L F Peruski
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620
    Biochim Biophys Acta 1207:165-72. 1994
    ..5 is nearly constitutive, increasing slightly with growth rate in media of different composition, and (iii) this protein is essential for growth at high temperature...
  2. ncbi Effects of the cys mutations on structure and function of the ATP-dependent HslVU protease in Escherichia coli. The Cys287 to Val mutation in HslU uncouples the ATP-dependent proteolysis by HslvU from ATP hydrolysis
    S J Yoo
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 273:22929-35. 1998
    ..HslVU protease, mutagenesis was performed to replace either Cys261 or Cys287 in HslU with Val and Cys159 in HslV with Ser or Ala...
  3. ncbi Crystal structure determination of Escherichia coli ClpP starting from an EM-derived mask
    J Wang
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520 8114, USA
    J Struct Biol 124:151-63. 1998
    ..Comparison of the ClpP structure with those of the 20S proteasome and HslV reveals a striking example of evolutionary convergence, despite them being unrelated in sequence and fold...
  4. pmc Redundant in vivo proteolytic activities of Escherichia coli Lon and the ClpYQ (HslUV) protease
    W F Wu
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892 4255, USA
    J Bacteriol 181:3681-7. 1999
    ..Thus, a protease with a structure and an active site different from those of Lon is capable of recognizing and degrading two different Lon substrates and appears to act as a backup for Lon under certain conditions...
  5. ncbi ATP-dependent degradation of SulA, a cell division inhibitor, by the HslVU protease in Escherichia coli
    I S Seong
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, South Korea
    FEBS Lett 456:211-4. 1999
    HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase...
  6. ncbi Effect of ELF magnetic fields on protein synthesis in Escherichia coli K12
    S Nakasono
    Bio Science Department, Abiko Research Laboratory, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko City, Chiba 270 1194, Japan
    Radiat Res 154:208-16. 2000
    ..in the levels of synthesis of the stress proteins were observed in heat-shocked cells (CH60, CH10, HTPG, DNAK, HSLV, IBPA and some unidentified proteins) and in cells grown under anaerobic conditions (DNAK, PFLB, RECA, USPA and ..
  7. pmc Mutational studies on HslU and its docking mode with HslV
    H K Song
    Abteilung Strukturforschung, Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Planegg Martinsried, Germany
    Proc Natl Acad Sci U S A 97:14103-8. 2000
    ..Despite detailed crystal and molecular structure determinations of free HslV and HslU, the mechanism of ATP-dependent peptide and protein hydrolysis remained unclear, mainly because the ..
  8. pmc The ATP-dependent CodWX (HslVU) protease in Bacillus subtilis is an N-terminal serine protease
    M S Kang
    School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    EMBO J 20:734-42. 2001
    HslVU is a two-component ATP-dependent protease, consisting of HslV peptidase and HslU ATPase...
  9. ncbi Crystal structures of the HslVU peptidase-ATPase complex reveal an ATP-dependent proteolysis mechanism
    J Wang
    Department of Molecular Biophysics, Biochemistry, 266 Whitney Avenue, Yale University, 06520, New Haven, CT, USA
    Structure 9:177-84. 2001
    The bacterial heat shock locus HslU ATPase and HslV peptidase together form an ATP-dependent HslVU protease. Bacterial HslVU is a homolog of the eukaryotic 26S proteasome...
  10. ncbi A corrected quaternary arrangement of the peptidase HslV and atpase HslU in a cocrystal structure
    J Wang
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, Connecticut 06520 8114, USA
    J Struct Biol 134:15-24. 2001
    The bacterial heat shock locus HslU ATPase and HslV peptidase together form an ATP-dependent HslVU protease...
  11. ncbi PCR genome walking identifies a genetic locus comprising two heat shock genes (hslV and hslU) from Leptospira borgpetersenii serovar hardjobovis
    M Lin
    Canadian Food Inspection Agency, Animal Diseases Research Institute, 3851 Fallowfield Road, Nepean, Ontario, Canada K2H 8P9
    Curr Microbiol 43:452-6. 2001
    ..of Leptospira borgpetersenii serovar hardjobovis has identified a genetic locus comprising two heat shock genes (hslV and hslU). This is the first molecular study on hslV and hslU in a Leptospira species...
  12. ncbi Nucleotide-dependent conformational changes in a protease-associated ATPase HsIU
    J Wang
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520, USA
    Structure 9:1107-16. 2001
    ..Nucleotide is required for the formation of the biologically active HslU hexameric assembly. The hexameric HslU ATPase binds the dodecameric HslV peptidase and forms an ATP-dependent HslVU protease.
  13. ncbi The C-terminal tails of HslU ATPase act as a molecular switch for activation of HslV peptidase
    Ihn Sik Seong
    National Research Laboratory of Protein Biochemistry, School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 277:25976-82. 2002
    ..HslU ATPase forms a hexameric ring, and HslV peptidase is a dodecamer consisting of two stacked hexameric rings...
  14. ncbi Eubacterial HslV and HslU subunits homologs in primordial eukaryotes
    Bernard Couvreur
    Laboratory for Protozoology, Prince Leopold Institute for Tropical Medicine, Antwerp, Belgium
    Mol Biol Evol 19:2110-7. 2002
    ..We showed that genes homologous to eubacterial HslV (ClpQ) and HslU (ClpY) are present in the genome of trypanosomatid protozoa and are expressed...
  15. pmc Degradation of mutant initiator protein DnaA204 by proteases ClpP, ClpQ and Lon is prevented when DNA is SeqA-free
    Monika Slominska
    Department of Cell Biology, Institute for Cancer Research, Montebello, 0310 Oslo, Norway
    Biochem J 370:867-71. 2003
    ..Its cognate protease ClpQ, as well as Lon protease, degraded DnaA204 to the same degree as ClpP. The chaperones GroES, GroEL and DnaK contributed to stabilization of DnaA204 protein...
  16. ncbi A comprehensive view on proteasomal sequences: implications for the evolution of the proteasome
    Christoph Gille
    Institute of Biochemistry, Medical Faculty Charité, Humboldt University, D 10117, Berlin, Germany
    J Mol Biol 326:1437-48. 2003
    ..tertiary structure: the 20S proteasome and the proteasome-like heat shock protein encoded by heat shock locus V, hslV. Usually, each biological kingdom is characterized by its specific type of proteasome...
  17. ncbi Overexpression of the hslVU operon suppresses SOS-mediated inhibition of cell division in Escherichia coli
    M M Khattar
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    FEBS Lett 414:402-4. 1997
    ..Further characterisation of pHL1 revealed that resistance to nitrofurantoin was due to the overexpression of the hslV-hslU operon which encodes an ATP-dependent protease complex in E. coli...
  18. ncbi ATP binding, but not its hydrolysis, is required for assembly and proteolytic activity of the HslVU protease in Escherichia coli
    S J Yoo
    College of Natural Sciences, Seoul National University, Seoul, 151 742, Korea
    Biochem Biophys Res Commun 238:581-5. 1997
    HslVU is an ATP-dependent protease consisting of two multimeric components: the HslU ATPase and the HslV peptidase...
  19. ncbi Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli
    S E Chuang
    Laboratory of Genetics, University of Wisconsin Madison 53706
    Gene 134:1-6. 1993
    ..Located at 88.9 min, the hslVU operon specifies proteins of 19.1 kDa (HslV) and 49.6 kDa (HslU). Multiple tsp were found in this operon...
  20. pmc HslV-HslU: A novel ATP-dependent protease complex in Escherichia coli related to the eukaryotic proteasome
    M Rohrwild
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 93:5808-13. 1996
    ..It is the product of the heat-shock locus hslVU that encodes two proteins: HslV, a 19-kDa protein similar to proteasome beta subunits, and HslU, a 50-kDa protein related to the ATPase ClpX...
  21. ncbi Purification and characterization of the heat shock proteins HslV and HslU that form a new ATP-dependent protease in Escherichia coli
    S J Yoo
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 271:14035-40. 1996
    The hslVU operon in Escherichia coli encodes two heat shock proteins, HslV, a 19-kDa protein homologous to beta-type subunits of the 20 S proteasomes, and HslU, a 50-kDa protein related to the ATPase ClpX...
  22. ncbi Molecular characterization of the xerC gene of Lactobacillus leichmannii encoding a site-specific recombinase and two adjacent heat shock genes
    J Becker
    Institut fur Mikrobiologie, J W Goethe Universitat, Frankfurt Main, Germany
    Curr Microbiol 32:232-6. 1996
    ..acid sequences of the complete ORFs showed considerable similarities with the already known sequences of the xerC, hslV, and hslU gene products of Escherichia coli: the site-specific XerC recombinase, a member of the lambda integrase ..
  23. ncbi Poly-L-lysine activates both peptide and ATP hydrolysis by the ATP-dependent HslVU protease in Escherichia coli
    S J Yoo
    Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea
    Biochem Biophys Res Commun 229:531-5. 1996
    ..Hs1VU in E. coli is a new type of ATP-dependent protease composed of two heat shock proteins, the HslU ATPase and the HslV peptidase related to certain beta-type subunits of the 20S proteasome...
  24. ncbi Mutational analysis of the ATP-binding site in HslU, the ATPase component of HslVU protease in Escherichia coli
    D H Shin
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, South Korea
    FEBS Lett 398:151-4. 1996
    ..hydrolyze ATP or support the ATP-dependent hydrolysis of N-carbobenzoxy-Gly-Gly-Leu-7-amido-4-methyl coumarin by HslV. The wild-type HslU (a mixture of monomer and dimer) formed a multimer containing 6-8 subunits in the presence of ..
  25. ncbi Six-fold rotational symmetry of ClpQ, the E. coli homolog of the 20S proteasome, and its ATP-dependent activator, ClpY
    M Kessel
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, MD 20892, USA
    FEBS Lett 398:274-8. 1996
    ClpQ (HslV) is a homolog of the beta-subunits of the 20S proteasome. In E. coli, it is expressed from an operon that also encodes ClpY (HslU), an ATPase homologous to the protease chaperone, ClpX...
  26. pmc Identification and characterization of HsIV HsIU (ClpQ ClpY) proteins involved in overall proteolysis of misfolded proteins in Escherichia coli
    D Missiakas
    Centre National de Recherche Scientifique, LIDSM CBBM, Marseille, France
    EMBO J 15:6899-909. 1996
    ..One such locus was identified and shown to carry the recently sequenced hslV hslU (clpQ clpY) operon...
  27. ncbi The ATP-dependent HslVU protease from Escherichia coli is a four-ring structure resembling the proteasome
    M Rohrwild
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Struct Biol 4:133-9. 1997
    ..We have used electron microscopy and image analysis to examine the structural organization of HslV and HslU homo-oligomers and the active HslVU enzyme...
  28. ncbi New insights into the mechanisms and importance of the proteasome in intracellular protein degradation
    A L Goldberg
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biol Chem 378:131-40. 1997
    ..HslVU is a two-component ATP-dependent protease composed of the proteasome-related peptidase HslV (beta-subunit) and the ATPase HslU...
  29. pmc Crystal structure of heat shock locus V (HslV) from Escherichia coli
    M Bochtler
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Proc Natl Acad Sci U S A 94:6070-4. 1997
    Heat shock locus V (HslV; also called ClpQ) is the proteolytic core of the ATP-dependent protease HslVU in Escherichia coli. It has sequence similarity with the beta-type subunits of the eukaryotic and archaebacterial proteasomes...
  30. pmc Covalent modification of the active site threonine of proteasomal beta subunits and the Escherichia coli homolog HslV by a new class of inhibitors
    M Bogyo
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 94:6629-34. 1997
    ..125I-NIP-L3VS covalently modifies the HslV subunit of the Escherichia coli protease complex HslV/HslU, a reaction that requires ATP, and supports a catalytic ..
  31. ncbi Mutagenesis of two N-terminal Thr and five Ser residues in HslV, the proteolytic component of the ATP-dependent HslVU protease
    S J Yoo
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, South Korea
    FEBS Lett 412:57-60. 1997
    ..coli is a new type of ATP-dependent protease consisting of two heat shock proteins: the HslU ATPase and the HslV peptidase that has two repeated Thr residues at its N terminus, like certain beta-type subunit of the 20S ..
  32. ncbi Proteolytic activity of the ATP-dependent protease HslVU can be uncoupled from ATP hydrolysis
    H Huang
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 272:21364-72. 1997
    HslVU is a new Escherichia coli ATP-dependent protease composed of two multimeric complexes: the HslU ATPase and the HslV peptidase. Prior studies indicated that HslVU requires ATP hydrolysis for the cleavage of peptides and proteins...
  33. ncbi The heat-shock protein HslVU from Escherichia coli is a protein-activated ATPase as well as an ATP-dependent proteinase
    J H Seol
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, Korea
    Eur J Biochem 247:1143-50. 1997
    ..coli a new two-component ATP-dependent protease composed of two heat-shock proteins, the HslU ATPase and the HslV peptidase which is related to proteasome beta-type subunits...
  34. pmc Subunit oligomerization and substrate recognition of the Escherichia coli ClpYQ (HslUV) protease implicated by in vivo protein-protein interactions in the yeast two-hybrid system
    Yi Ying Lee
    Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan
    J Bacteriol 185:2393-401. 2003
    ..Thus, we demonstrated in vivo hetero- and homointeractions of ClpQ and ClpY molecules, as well as a direct association between ClpY and substrate SulA, thereby supporting previous in vitro biochemical findings...