B Striepen

Summary

Affiliation: University of Georgia
Country: USA

Publications

  1. pmc A screening pipeline for antiparasitic agents targeting cryptosporidium inosine monophosphate dehydrogenase
    Lisa Sharling
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, United States of America
    PLoS Negl Trop Dis 4:e794. 2010
  2. pmc An apicoplast localized ubiquitylation system is required for the import of nuclear-encoded plastid proteins
    Swati Agrawal
    Department of Cellular Biology, University of Georgia, Athens, Georgia, United States of America
    PLoS Pathog 9:e1003426. 2013
  3. pmc Apicoplast isoprenoid precursor synthesis and the molecular basis of fosmidomycin resistance in Toxoplasma gondii
    Sethu C Nair
    Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    J Exp Med 208:1547-59. 2011
  4. ncbi request reprint Genomics meets transgenics in search of the elusive Cryptosporidium drug target
    Boris Striepen
    Center for Tropical and Emerging Global Diseases, University of Georgia, 623 Biological Sciences Building, Athens, GA 30602, USA
    Trends Parasitol 20:355-8. 2004
  5. pmc The plastid of Toxoplasma gondii is divided by association with the centrosomes
    B Striepen
    Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
    J Cell Biol 151:1423-34. 2000
  6. ncbi request reprint Targeting of soluble proteins to the rhoptries and micronemes in Toxoplasma gondii
    B Striepen
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Biochem Parasitol 113:45-53. 2001
  7. pmc Gene transfer in the evolution of parasite nucleotide biosynthesis
    Boris Striepen
    Center for Tropical and Emerging Global Diseases and Departments of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 101:3154-9. 2004
  8. pmc Building the perfect parasite: cell division in apicomplexa
    Boris Striepen
    Center for Tropical and Emerging Global Diseases and the Department of Cellular Biology, University of Georgia, Athens, Georgia, United States of America
    PLoS Pathog 3:e78. 2007
  9. pmc Genetic complementation in apicomplexan parasites
    Boris Striepen
    Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 99:6304-9. 2002
  10. ncbi request reprint Tagging genes and trapping promoters in Toxoplasma gondii by insertional mutagenesis
    D S Roos
    Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, Pennsylvania, 19104 6018, USA
    Methods 13:112-22. 1997

Research Grants

  1. Protein targeting and secretion in Toxoplasma
    Boris Striepen; Fiscal Year: 2004
  2. Ninth International Congress on Toxoplasmosis
    Boris Striepen; Fiscal Year: 2007
  3. IMPDH as a Drug Target in Cryptosporidium
    Boris Striepen; Fiscal Year: 2008

Collaborators

Detail Information

Publications49

  1. pmc A screening pipeline for antiparasitic agents targeting cryptosporidium inosine monophosphate dehydrogenase
    Lisa Sharling
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, United States of America
    PLoS Negl Trop Dis 4:e794. 2010
    ..While the genome sequencing effort has identified several intriguing new targets including a unique inosine monophosphate dehydrogenase (IMPDH), pursuing these targets and testing inhibitors has been frustratingly difficult...
  2. pmc An apicoplast localized ubiquitylation system is required for the import of nuclear-encoded plastid proteins
    Swati Agrawal
    Department of Cellular Biology, University of Georgia, Athens, Georgia, United States of America
    PLoS Pathog 9:e1003426. 2013
    ..Our studies suggest ubiquitylation to be a mechanistic requirement of apicoplast protein import independent to the proteasomal degradation pathway...
  3. pmc Apicoplast isoprenoid precursor synthesis and the molecular basis of fosmidomycin resistance in Toxoplasma gondii
    Sethu C Nair
    Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    J Exp Med 208:1547-59. 2011
    ..We propose that the varied extent of metabolite exchange between host and parasite is a crucial determinator of drug susceptibility and a predictor of future resistance...
  4. ncbi request reprint Genomics meets transgenics in search of the elusive Cryptosporidium drug target
    Boris Striepen
    Center for Tropical and Emerging Global Diseases, University of Georgia, 623 Biological Sciences Building, Athens, GA 30602, USA
    Trends Parasitol 20:355-8. 2004
    ..New targets for Cryptosporidium drug and vaccine development can be identified and validated based on this approach...
  5. pmc The plastid of Toxoplasma gondii is divided by association with the centrosomes
    B Striepen
    Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
    J Cell Biol 151:1423-34. 2000
    ..These observations are discussed in the context of autonomous and FtsZ-dependent division of plastids in plants and algae...
  6. ncbi request reprint Targeting of soluble proteins to the rhoptries and micronemes in Toxoplasma gondii
    B Striepen
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Biochem Parasitol 113:45-53. 2001
    ..GFP fusion proteins were readily secreted from dense granules, however, suggesting that protein secretion from rhoptries and micronemes might involve more than a simple release of organellar contents...
  7. pmc Gene transfer in the evolution of parasite nucleotide biosynthesis
    Boris Striepen
    Center for Tropical and Emerging Global Diseases and Departments of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 101:3154-9. 2004
    ..Taken together, these data help to explain why widely used drugs fail in the treatment of cryptosporidiosis and suggest more promising targets...
  8. pmc Building the perfect parasite: cell division in apicomplexa
    Boris Striepen
    Center for Tropical and Emerging Global Diseases and the Department of Cellular Biology, University of Georgia, Athens, Georgia, United States of America
    PLoS Pathog 3:e78. 2007
    ....
  9. pmc Genetic complementation in apicomplexan parasites
    Boris Striepen
    Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 99:6304-9. 2002
    ..The prokaryotic origin of this enzyme might make it a promising target for therapeutics directed against Cryptosporidium...
  10. ncbi request reprint Tagging genes and trapping promoters in Toxoplasma gondii by insertional mutagenesis
    D S Roos
    Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, Pennsylvania, 19104 6018, USA
    Methods 13:112-22. 1997
    ..Genetic strategies are also possible, using reporters whose function can be assayed by metabolic, visual, or immunological screens to "trap" genes that are activated (or inactivated) under various conditions of interest...
  11. ncbi request reprint Targeting and processing of nuclear-encoded apicoplast proteins in plastid segregation mutants of Toxoplasma gondii
    C Y He
    Department of Biology, Cancer Center Flow Cytometry Shared Resource, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 276:28436-42. 2001
    ..Taking advantage of the ability to isolate apicoplast segregation mutants, we also demonstrated that processing of the transit peptide of nuclear-encoded apicoplast proteins requires plastid-associated activity...
  12. pmc Forward genetic analysis of the apicomplexan cell division cycle in Toxoplasma gondii
    Marc Jan Gubbels
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, United States of America
    PLoS Pathog 4:e36. 2008
    ..The genes identified include regulators of spindle formation, nuclear trafficking, and protein degradation. The genetic approach described here should be widely applicable to numerous essential aspects of parasite biology...
  13. ncbi request reprint Origin, targeting, and function of the apicomplexan plastid
    D S Roos
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Curr Opin Microbiol 2:426-32. 1999
    ..Combining molecular genetic identification of targeting signals with whole genome analysis promises to yield a complete picture of organellar metabolic pathways and new targets for drug design...
  14. ncbi request reprint Cryptosporidium parvum IMP dehydrogenase: identification of functional, structural, and dynamic properties that can be exploited for drug design
    Nwakaso N Umejiego
    Department of Cellular Biology, Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 279:40320-7. 2004
    ..These data suggest that the structure and dynamic properties of the NAD binding site of C. parvum IMPDH can be exploited to develop parasite-specific inhibitors...
  15. pmc Mining the Plasmodium genome database to define organellar function: what does the apicoplast do?
    David S Roos
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Philos Trans R Soc Lond B Biol Sci 357:35-46. 2002
    ....
  16. ncbi request reprint Interleukin-10 does not contribute to the pathogenesis of a virulent strain of Toxoplasma gondii
    U Wille
    Department of Pathobiology, University of Pennsylvania, School of Veterinary Medicine, Philadelphia, PA, USA
    Parasite Immunol 23:291-6. 2001
    ..Taken together, these results indicate that IL-10 is not required for the virulence of the RH strain of T. gondii, nor is it involved in the regulation of the CD80, CD86 and MHC class II molecules during RH-infection...
  17. ncbi request reprint Fluorescent protein tagging in Toxoplasma gondii: identification of a novel inner membrane complex component conserved among Apicomplexa
    Marc Jan Gubbels
    Center for Tropical and Emerging Global Diseases, University of Georgia, 724 Biological Sciences Building, Athens 30602, USA
    Mol Biochem Parasitol 137:99-110. 2004
    ..Among the genes identified is a novel inner membrane complex gene (IMC3) conserved among Apicomplexa...
  18. ncbi request reprint Plastid segregation and cell division in the apicomplexan parasite Sarcocystis neurona
    Shipra Vaishnava
    Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    J Cell Sci 118:3397-407. 2005
    ..Finally, plastids were labeled by fluorescent protein expression using a newly developed S. neurona transfection system. With these transgenic parasites we have tested our model in living cells employing laser bleaching experiments...
  19. ncbi request reprint A MORN-repeat protein is a dynamic component of the Toxoplasma gondii cell division apparatus
    Marc Jan Gubbels
    Center for Tropical and Emerging Global Diseases, University of Georgia, Paul D Coverdell Center, Athens, 30602, USA
    J Cell Sci 119:2236-45. 2006
    ..Our initial biochemical analysis is consistent with this model. Whereas recombinant MORN1 produced in bacteria is soluble, in the parasite MORN1 was associated with the cytoskeleton after detergent extraction...
  20. pmc Genetic evidence that an endosymbiont-derived endoplasmic reticulum-associated protein degradation (ERAD) system functions in import of apicoplast proteins
    Swati Agrawal
    Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 284:33683-91. 2009
    ..We engineered a conditional null mutant in apicoplast Der1, the putative pore of the apicoplast ERAD complex, and found that loss of Der1(Ap) results in loss of apicoplast protein import and subsequent death of the parasite...
  21. pmc A novel dynamin-related protein has been recruited for apicoplast fission in Toxoplasma gondii
    Giel G van Dooren
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, 30602, USA
    Curr Biol 19:267-76. 2009
    ..Apicoplasts lack the conserved machinery that divides chloroplasts in plants and red algae, suggesting that these mechanisms are unique...
  22. pmc High-throughput growth assay for Toxoplasma gondii using yellow fluorescent protein
    Marc Jan Gubbels
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
    Antimicrob Agents Chemother 47:309-16. 2003
    ....
  23. pmc Daughter cell assembly in the protozoan parasite Toxoplasma gondii
    Ke Hu
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Biol Cell 13:593-606. 2002
    ..gondii is also capable of forming multiple daughters, suggesting fundamental similarities between cell division in T. gondii and other apicomplexan parasites...
  24. ncbi request reprint Studying the cell biology of apicomplexan parasites using fluorescent proteins
    Marc Jan Gubbels
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Microsc Microanal 10:568-79. 2004
    ..The combination of the in vivo marker GFP with an increasingly diverse genetic toolbox for T. gondii opens many exciting experimental opportunities, and emerging applications of GFP in genetic and pharmacological screens are discussed...
  25. pmc Toxoplasma gondii Tic20 is essential for apicoplast protein import
    Giel G van Dooren
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 105:13574-9. 2008
    ..Our studies provide experimental evidence for a common evolutionary origin of import mechanisms across the innermost membranes of primary and secondary plastids...
  26. ncbi request reprint The cell biology of secondary endosymbiosis--how parasites build, divide and segregate the apicoplast
    Shipra Vaishnava
    Department of Cellular Biology, University of Georgia, Paul D Coverdell Center, 500 D W Brooks Drive, Athens, GA 30602, USA
    Mol Microbiol 61:1380-7. 2006
    ..We discuss how these resources might be marshalled to develop a detailed mechanistic picture of apicoplast cell biology...
  27. pmc Apicoplast fatty acid synthesis is essential for organelle biogenesis and parasite survival in Toxoplasma gondii
    Jolly Mazumdar
    Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Paul D Coverdell Center, 500 D W Brooks Drive, Athens, 30602, USA
    Proc Natl Acad Sci U S A 103:13192-7. 2006
    ..Our genetic model also offers a platform to dissect the integration of the apicoplast into parasite metabolism, especially its postulated interaction with the mitochondrion...
  28. ncbi request reprint Isolation and characterization of TgVP1, a type I vacuolar H+-translocating pyrophosphatase from Toxoplasma gondii. The dynamics of its subcellular localization and the cellular effects of a diphosphonate inhibitor
    Yolanda M Drozdowicz
    Department of Biology, University of Pennsylvania, Philadelphia 19104, USA
    J Biol Chem 278:1075-85. 2003
    ..A dynamic association of TgVP1 with the host cell invasion apparatus is invoked, one in which the effects of inhibitory V-PPase substrate analogs are exerted after rather than during host cell invasion...
  29. pmc Make it or take it: fatty acid metabolism of apicomplexan parasites
    Jolly Mazumdar
    Department of Cellular Biology, University of Georgia, Paul D Coverdell Center, Athens, GA 30602, USA
    Eukaryot Cell 6:1727-35. 2007
  30. pmc A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis
    Alexander Lorestani
    Department of Biology, Boston College, Chestnut Hill, Massachusetts, United States of America
    PLoS ONE 5:e12302. 2010
    ..In conclusion, MORN1 is required for basal complex assembly and loss of MORN1 results in defects in apicoplast division and daughter segregation...
  31. pmc The toxoplasma apicoplast phosphate translocator links cytosolic and apicoplast metabolism and is essential for parasite survival
    Carrie F Brooks
    Center for Tropical and Emerging Global Diseases, University of Georgia, Paul D Coverdell Center, 500 D W Brooks Drive, Athens, GA 30602, USA
    Cell Host Microbe 7:62-73. 2010
    ..Genetic ablation of the transporter results in rapid death of parasites. The dramatic consequences of loss of its activity suggest that targeting TgAPT could be a viable strategy to identify antiparasitic compounds...
  32. ncbi request reprint Adenosine kinase from Cryptosporidium parvum
    Jon Galazka
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97239 3098, USA
    Mol Biochem Parasitol 149:223-30. 2006
    ..The overproduction and purification of CpAK now enables a thorough evaluation of its potential as a drug target...
  33. pmc Targeting a prokaryotic protein in a eukaryotic pathogen: identification of lead compounds against cryptosporidiosis
    Nwakaso N Umejiego
    Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    Chem Biol 15:70-7. 2008
    ..We have identified four parasite-selective IMPDH inhibitors that display antiparasitic activity with greater potency than paromomycin, the current gold standard for anticryptosporidial activity...
  34. ncbi request reprint cDNA cloning and expression of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase T1 from Toxoplasma gondii
    Boguslaw S Wojczyk
    Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Box 626, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Mol Biochem Parasitol 131:93-107. 2003
    ..The identification of this enzyme in T. gondii demonstrates that this human parasite has its own enzymatic machinery for the O-glycosylation of toxoplasmal proteins...
  35. ncbi request reprint Identification of a sporozoite-specific member of the Toxoplasma SAG superfamily via genetic complementation
    Jay R Radke
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717 3610, USA
    Mol Microbiol 52:93-105. 2004
    ..Expression of SporoSAG in tachyzoites conferred enhanced invasion on transgenic parasites suggesting a role for this protein in oocyst/sporozoite transmission to susceptible hosts...
  36. ncbi request reprint Molecular genetic transfection of the coccidian parasite Sarcocystis neurona
    Rajshekhar Y Gaji
    108 M H Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, USA
    Mol Biochem Parasitol 150:1-9. 2006
    ..More importantly, the DNA transfection methods described herein should greatly facilitate studies examining intracellular parasitism by this important coccidian pathogen...
  37. pmc IMP dehydrogenase from the protozoan parasite Toxoplasma gondii
    William J Sullivan
    Department of Pharmacology and Toxicology, Medical Sciences Building Room A 519, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202 5120, USA
    Antimicrob Agents Chemother 49:2172-9. 2005
    ....
  38. ncbi request reprint Expression variance, biochemical and immunological properties of Toxoplasma gondii dense granule protein GRA7
    Anja Neudeck
    Institute for Medical Microbiology and Virology, Heinrich Heine University, Universitätsstrasse 1 Geb 22 21, 40225 Dusseldorf, Germany
    Microbes Infect 4:581-90. 2002
    ..Consistent with a role at a parasite-host interface, GRA7 proved to be a target antigen of the intracerebral immune response as evidenced by the presence of GRA7-specific antibodies in mouse cerebrospinal fluid during chronic infection...
  39. pmc Dynamics of neutrophil migration in lymph nodes during infection
    Tatyana Chtanova
    Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, CA 94720, USA
    Immunity 29:487-96. 2008
    ..Our data provide insights into the cellular mechanisms underlying neutrophil swarming and suggest new roles for neutrophils in shaping immune responses...
  40. doi request reprint Deploying parasite profilin on a mission of invasion and danger
    Eric Y Denkers
    Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Cell Host Microbe 3:61-3. 2008
    ....
  41. ncbi request reprint Analysis of the Sarcocystis neurona microneme protein SnMIC10: protein characteristics and expression during intracellular development
    Jessica S Hoane
    Department of Veterinary Science, University of Kentucky, 108 Gluck Equine Research Center, Lexington, KY 40546 0099, USA
    Int J Parasitol 33:671-9. 2003
    ..Identification and characterisation of additional S. neurona microneme antigens and comparisons to orthologues in other Apicomplexa could provide further insight into the functions that these proteins serve during invasion of host cells...
  42. pmc Class I major histocompatibility complex presentation of antigens that escape from the parasitophorous vacuole of Toxoplasma gondii
    Marc Jan Gubbels
    Department of Molecular and Cell Biology, 471 Life Sciences Addition, University of California Berkeley, Berkeley, CA 94720, USA
    Infect Immun 73:703-11. 2005
    ....
  43. pmc CD40 induces macrophage anti-Toxoplasma gondii activity by triggering autophagy-dependent fusion of pathogen-containing vacuoles and lysosomes
    Rosa M Andrade
    Division of Infectious Diseases, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    J Clin Invest 116:2366-77. 2006
    ..Thus, cell-mediated immunity through CD40 stimulation can reroute an intracellular pathogen to the lysosomal compartment, resulting in macrophage antimicrobial activity...
  44. ncbi request reprint Intraepithelial gammadelta+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection
    Jane E Dalton
    Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom
    Gastroenterology 131:818-29. 2006
    ..We have investigated the role intestinal intraepithelial lymphocytes (iIELs) play in regulating epithelial permeability in response to infection...
  45. ncbi request reprint A requirement for the Vgamma1+ subset of peripheral gammadelta T cells in the control of the systemic growth of Toxoplasma gondii and infection-induced pathology
    Charlotte E Egan
    Research Institute for Molecular and Cellular Biology, University of Leeds, UK
    J Immunol 175:8191-9. 2005
    ....
  46. ncbi request reprint Plastid-targeting peptides from the chlorarachniophyte Bigelowiella natans
    Matthew B Rogers
    Canadian Institute for Advanced Research, Department of Botany, University of British Columbia, 3529 6270 University Boulevard, Vancouver, BC, V6T 1Z4, Canada
    J Eukaryot Microbiol 51:529-35. 2004
    ..Consistent with this, the leader sequence from one B. natans protein was tested for function in the apicomplexan parasite, Toxoplasma gondii, and shown to direct the secretion of the protein...
  47. ncbi request reprint Genetic rescue of a Toxoplasma gondii conditional cell cycle mutant
    Michael W White
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717 3610, USA
    Mol Microbiol 55:1060-71. 2005
    ..Altogether, these results demonstrate that ts11C9 is a conditional mitotic mutant containing a single defect which influences two distinct control points in the T. gondii tachyzoite cell cycle...
  48. pmc Sarcocystis neurona merozoites express a family of immunogenic surface antigens that are orthologues of the Toxoplasma gondii surface antigens (SAGs) and SAG-related sequences
    Daniel K Howe
    Department of Veterinary Science, 108 Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546 0099, USA
    Infect Immun 73:1023-33. 2005
    ..neurona indicates that expression of multiple related surface antigens is not unique to the ubiquitous organism T. gondii. Instead, the SAG gene family is a common trait that presumably has an essential, conserved function(s)...
  49. ncbi request reprint Switching parasite proteins on and off
    Boris Striepen
    Nat Methods 4:999-1000. 2007

Research Grants3

  1. Protein targeting and secretion in Toxoplasma
    Boris Striepen; Fiscal Year: 2004
    ..Mutant analysis will also provide critical information towards the function of the individual secretory organelles involved. ..
  2. Ninth International Congress on Toxoplasmosis
    Boris Striepen; Fiscal Year: 2007
    ..gondii that is not often seen in fields as high paced and[unreadable] competitive and has facilitated rapid and sustained progress in research on this pathogen[unreadable] and related parasites. ..
  3. IMPDH as a Drug Target in Cryptosporidium
    Boris Striepen; Fiscal Year: 2008
    ..This work will provide urgently needed molecular reagents to understand the biology of nutrient uptake and drug resistance in this unique parasite. [unreadable] [unreadable] [unreadable]..