Patrick L Sinn

Summary

Affiliation: University of Iowa
Country: USA

Publications

  1. pmc Measles virus preferentially transduces the basolateral surface of well-differentiated human airway epithelia
    Patrick L Sinn
    Department of Pediatrics, Program in Gene Therapy, The University of Iowa College of Medicine, Iowa City, IA 52242, USA
    J Virol 76:2403-9. 2002
  2. ncbi request reprint Gene transfer to respiratory epithelia with lentivirus pseudotyped with Jaagsiekte sheep retrovirus envelope glycoprotein
    Patrick L Sinn
    Program in Gene Therapy, Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
    Hum Gene Ther 16:479-88. 2005
  3. ncbi request reprint Enhanced gene expression conferred by stepwise modification of a nonprimate lentiviral vector
    Patrick L Sinn
    Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Hum Gene Ther 18:1244-52. 2007
  4. pmc Measles virus blind to its epithelial cell receptor remains virulent in rhesus monkeys but cannot cross the airway epithelium and is not shed
    Vincent H J Leonard
    Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, USA
    J Clin Invest 118:2448-58. 2008
  5. pmc Intrapulmonary Versus Nasal Transduction of Murine Airways With GP64-pseudotyped Viral Vectors
    Mayumi Oakland
    Department of Microbiology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA
    Mol Ther Nucleic Acids 2:e69. 2013
  6. pmc Lentiviral vector gene transfer to porcine airways
    Patrick L Sinn
    Department of Pediatrics, Center for Gene Therapy of Cystic Fibrosis and Other Genetic Diseases, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA
    Mol Ther Nucleic Acids 1:e56. 2012
  7. pmc Lentivirus vector can be readministered to nasal epithelia without blocking immune responses
    Patrick L Sinn
    Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
    J Virol 82:10684-92. 2008
  8. pmc Lentivirus vectors pseudotyped with filoviral envelope glycoproteins transduce airway epithelia from the apical surface independently of folate receptor alpha
    Patrick L Sinn
    Program in Gene Therapy, Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Virol 77:5902-10. 2003
  9. pmc Transcriptional targeting in the airway using novel gene regulatory elements
    Erin R Burnight
    Interdisciplinary Graduate Program in Genetics, Carver College of Medicine, University of Iowa, Iowa City, 52242, USA
    Am J Respir Cell Mol Biol 47:227-33. 2012
  10. pmc Persistent gene expression in mouse nasal epithelia following feline immunodeficiency virus-based vector gene transfer
    Patrick L Sinn
    Program in Gene Therapy, Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, 52242, USA
    J Virol 79:12818-27. 2005

Collaborators

Detail Information

Publications27

  1. pmc Measles virus preferentially transduces the basolateral surface of well-differentiated human airway epithelia
    Patrick L Sinn
    Department of Pediatrics, Program in Gene Therapy, The University of Iowa College of Medicine, Iowa City, IA 52242, USA
    J Virol 76:2403-9. 2002
    ..These data suggest that the progression of MV infection through the respiratory epithelium may involve pathways other than direct binding and entry through the apical surface of airway epithelia...
  2. ncbi request reprint Gene transfer to respiratory epithelia with lentivirus pseudotyped with Jaagsiekte sheep retrovirus envelope glycoprotein
    Patrick L Sinn
    Program in Gene Therapy, Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
    Hum Gene Ther 16:479-88. 2005
    ....
  3. ncbi request reprint Enhanced gene expression conferred by stepwise modification of a nonprimate lentiviral vector
    Patrick L Sinn
    Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Hum Gene Ther 18:1244-52. 2007
    ..These sequential modifications provide an improved FIV lentivirus platform for gene therapy applications and may be applied to other retroviral vectors...
  4. pmc Measles virus blind to its epithelial cell receptor remains virulent in rhesus monkeys but cannot cross the airway epithelium and is not shed
    Vincent H J Leonard
    Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, USA
    J Clin Invest 118:2448-58. 2008
    ..Thus, EpR is probably a basolateral protein, and infection of the airway epithelium is not essential for systemic spread and virulence of MV...
  5. pmc Intrapulmonary Versus Nasal Transduction of Murine Airways With GP64-pseudotyped Viral Vectors
    Mayumi Oakland
    Department of Microbiology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA
    Mol Ther Nucleic Acids 2:e69. 2013
    ..Molecular Therapy - Nucleic Acids (2013) 2, e69; doi:10.1038/mtna.2012.60; published online 29 January 2013...
  6. pmc Lentiviral vector gene transfer to porcine airways
    Patrick L Sinn
    Department of Pediatrics, Center for Gene Therapy of Cystic Fibrosis and Other Genetic Diseases, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA
    Mol Ther Nucleic Acids 1:e56. 2012
    ..Molecular Therapy - Nucleic Acids (2012) 1, e56; doi:10.1038/mtna.2012.47; published online 27 November 2012...
  7. pmc Lentivirus vector can be readministered to nasal epithelia without blocking immune responses
    Patrick L Sinn
    Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
    J Virol 82:10684-92. 2008
    ..Prolonged gene expression has been observed in vivo following a single dose of virus vector; however, depending on the application, repeated administration of vector may be necessary to achieve stable, therapeutic gene expression...
  8. pmc Lentivirus vectors pseudotyped with filoviral envelope glycoproteins transduce airway epithelia from the apical surface independently of folate receptor alpha
    Patrick L Sinn
    Program in Gene Therapy, Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Virol 77:5902-10. 2003
    ..Understanding viral vector binding and entry pathways is fundamental for developing cystic fibrosis gene therapy applications...
  9. pmc Transcriptional targeting in the airway using novel gene regulatory elements
    Erin R Burnight
    Interdisciplinary Graduate Program in Genetics, Carver College of Medicine, University of Iowa, Iowa City, 52242, USA
    Am J Respir Cell Mol Biol 47:227-33. 2012
    ..These results will guide the development of optimal vectors for sufficient, sustained CFTR expression in airway epithelia...
  10. pmc Persistent gene expression in mouse nasal epithelia following feline immunodeficiency virus-based vector gene transfer
    Patrick L Sinn
    Program in Gene Therapy, Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, 52242, USA
    J Virol 79:12818-27. 2005
    ..These data suggest that AcGP64-FIV efficiently transduces and persistently expresses a transgene in nasal epithelia in the absence of agents that disrupt the cellular tight junction integrity...
  11. pmc In vivo gene transfer using a nonprimate lentiviral vector pseudotyped with Ross River Virus glycoproteins
    Yubin Kang
    Program in Gene Therapy, Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Virol 76:9378-88. 2002
    ..Our data support the utility of RRV glycoprotein-pseudotyped FIV lentiviral vectors for hepatocyte- and neuroglia-related disease applications...
  12. pmc Tyrosine kinase receptor Axl enhances entry of Zaire ebolavirus without direct interactions with the viral glycoprotein
    Melinda A Brindley
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    Virology 415:83-94. 2011
    ..In total, these findings indicate that endosomal uptake of filoviruses is facilitated by Axl...
  13. ncbi request reprint Cells of respiratory epithelium
    Patrick L Sinn
    Department of Pediatrics, University of Iowa, College of Medicine, Iowa City, USA
    Methods Mol Biol 229:287-98. 2003
  14. ncbi request reprint Ferret and Pig Models of Cystic Fibrosis: Prospects and Promise for Gene Therapy
    Ziying Yan
    University of Iowa, Anatomy and Cell Biology, Iowa City, Iowa, United States, University of Iowa, Center for Gene Therapy of Cystic Fibrosis, Iowa City, Iowa, United States
    Hum Gene Ther Clin Dev . 2014
    ..Through this review, we hope to convey the potential of these new animal models for developing CF gene and cell therapies...
  15. ncbi request reprint Inclusion of Jaagsiekte sheep retrovirus proviral elements markedly increases lentivirus vector pseudotyping efficiency
    Patrick L Sinn
    Department of Pediatrics, Program in Gene Therapy, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
    Mol Ther 11:460-9. 2005
    ..These novel data demonstrate that optimization of the envelope expression construct can profoundly influence titers for lentivirus vectors...
  16. pmc Cell-to-Cell Contact and Nectin-4 Govern Spread of Measles Virus from Primary Human Myeloid Cells to Primary Human Airway Epithelial Cells
    Brajesh K Singh
    Stead Family Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA
    J Virol 90:6808-17. 2016
    ..Thus, both infected MDMs and DCs rely on cell-to-cell contacts and nectin-4 to efficiently deliver MeV to the basolateral surface of HAE...
  17. pmc Advances in cell and gene-based therapies for cystic fibrosis lung disease
    Mayumi Oakland
    Department of Microbiology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
    Mol Ther 20:1108-15. 2012
    ..This review discusses current challenges and advancements in viral and nonviral vectors, cell-based therapies, and CF animal models...
  18. ncbi request reprint Gene transfer to airway epithelia using feline immunodeficiency virus-based lentivirus vectors
    Guoshun Wang
    Departments of Pediatrics and Internal Medicine, Program in Gene Therapy, College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    Methods Enzymol 346:500-14. 2002
  19. ncbi request reprint Viscoelastic gel formulations enhance airway epithelial gene transfer with viral vectors
    Patrick L Sinn
    Department of Pediatrics, 240G EMRB, The University of Iowa, Iowa City, IA 52242, USA
    Am J Respir Cell Mol Biol 32:404-10. 2005
    ..Importantly, viscoelastic gel formulations enhanced vector transduction to the conducting airways, the desired treatment target for diseases such as cystic fibrosis...
  20. pmc Genetic therapies for cystic fibrosis lung disease
    Patrick L Sinn
    Program in Gene Therapy, Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
    Hum Mol Genet 20:R79-86. 2011
    ..Here, we review recent developments that are advancing this field of investigation...
  21. pmc Human, Pig, and Mouse Interferon-Induced Transmembrane Proteins Partially Restrict Pseudotyped Lentiviral Vectors
    Andrew L Hornick
    Stead Family Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, Iowa
    Hum Gene Ther 27:354-62. 2016
    ..The findings are relevant to future preclinical and clinical airway gene therapy trials using lentivirus-based vectors. ..
  22. pmc The Nectin-4/Afadin Protein Complex and Intercellular Membrane Pores Contribute to Rapid Spread of Measles Virus in Primary Human Airway Epithelia
    Brajesh K Singh
    Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA
    J Virol 89:7089-96. 2015
    ..Virus-induced intercellular pores may contribute to extremely efficient measles contagion by promoting the rapid spread of the virus through the upper respiratory epithelium...
  23. pmc Lentiviral-mediated phenotypic correction of cystic fibrosis pigs
    Ashley L Cooney
    Pappajohn Biomedical Institute, The University of Iowa, Iowa City, Iowa, USA Roy J and Lucille A Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA Department of Microbiology, The University of Iowa, Iowa City, Iowa, USA
    JCI Insight 1:. 2016
    ....
  24. pmc Hybrid nonviral/viral vector systems for improved piggyBac DNA transposon in vivo delivery
    Ashley L Cooney
    Department of Microbiology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA
    Mol Ther 23:667-74. 2015
    ....
  25. pmc Ferret and pig models of cystic fibrosis: prospects and promise for gene therapy
    Ziying Yan
    1 Department of Anatomy and Cell Biology, University of Iowa School of Medicine, Iowa City, IA 52242
    Hum Gene Ther Clin Dev 26:38-49. 2015
    ..Through this review, we hope to convey the potential of these new animal models for developing CF gene and cell therapies. ..
  26. pmc T-cell immunoglobulin and mucin domain 1 (TIM-1) is a receptor for Zaire Ebolavirus and Lake Victoria Marburgvirus
    Andrew S Kondratowicz
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 108:8426-31. 2011
    ..ARD5, a monoclonal antibody against the IgV domain of TIM-1, blocked EBOV binding and infection, suggesting that antibodies or small molecules directed against this cellular receptor may provide effective filovirus antivirals...
  27. ncbi request reprint In vivo imaging of gene transfer to the respiratory tract
    Uta Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW3 6LR, UK
    Biomaterials 29:1533-40. 2008
    ..45, p<0.05). To our knowledge these studies show for the first time that this non-invasive method of assessing pulmonary gene transfer is viable for evaluating non-viral gene transfer agents...