Affiliation: University of Pennsylvania
Black B, Jansen L, Maddox P, Foltz D, Desai A, Shah J, et al
. Centromere identity maintained by nucleosomes assembled with histone H3 containing the CENP-A targeting domain. Mol Cell. 2007;25:309-22 pubmed
..These data offer direct support for centromere identity maintained by a unique nucleosome that serves to distinguish the centromere from the rest of the chromosome. ..
Logsdon G, Barrey E, Bassett E, Denizio J, Guo L, Panchenko T, et al
. Both tails and the centromere targeting domain of CENP-A are required for centromere establishment. J Cell Biol. 2015;208:521-31 pubmed publisher
..Our results indicate that the regions of CENP-A required for early events in centromere establishment differ from those that are required for maintaining centromere identity. ..
Dawicki McKenna J, Langelier M, Denizio J, Riccio A, Cao C, Karch K, et al
. PARP-1 Activation Requires Local Unfolding of an Autoinhibitory Domain. Mol Cell. 2015;60:755-768 pubmed publisher
..Our molecular model explains how PARP-1 DNA damage detection leads to local unfolding of the HD that relieves autoinhibition, and has important implications for the design of PARP inhibitors. ..
Smoak E, Stein P, Schultz R, Lampson M, Black B. Long-Term Retention of CENP-A Nucleosomes in Mammalian Oocytes Underpins Transgenerational Inheritance of Centromere Identity. Curr Biol. 2016;26:1110-6 pubmed publisher
..Thus, the remarkable stability of CENP-A nucleosomes confers transgenerational centromere identity in mammals. ..
Sekulic N, Black B. Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Nonhistone Centromere Proteins. Methods Enzymol. 2016;573:67-96 pubmed publisher
..Lastly, we describe our approach to assemble and analyze a complex between the recombinant human CENP-A nucleosome and one of its binding partners, CENP-C. ..
Falk S, Guo L, Sekulic N, Smoak E, Mani T, Logsdon G, et al
. Chromosomes. CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere. Science. 2015;348:699-703 pubmed publisher
..CENP-C depletion leads to rapid removal of CENP-A from centromeres, indicating their collaboration in maintaining centromere identity. ..
Stankovic A, Guo L, Mata J, Bodor D, Cao X, Bailey A, et al
. A Dual Inhibitory Mechanism Sufficient to Maintain Cell-Cycle-Restricted CENP-A Assembly. Mol Cell. 2017;65:231-246 pubmed publisher
..We find that Cdk-mediated inhibition is exerted by sequestering active factors away from the centromere. Finally, we show that displacement of M18BP1 from the centromere is critical for the assembly mechanism of CENP-A. ..
Fachinetti D, Logsdon G, Abdullah A, Selzer E, Cleveland D, Black B. CENP-A Modifications on Ser68 and Lys124 Are Dispensable for Establishment, Maintenance, and Long-Term Function of Human Centromeres. Dev Cell. 2017;40:104-113 pubmed publisher
..Thus, neither Ser68 nor Lys124 post-translational modification is essential for long-term centromere identity, propagation, cell-cycle-dependent deposition, maintenance, function, or mediation of early steps in centromere establishment. ..
Iwata Otsubo A, Dawicki McKenna J, Akera T, Falk S, Chmatal L, Yang K, et al
. Expanded Satellite Repeats Amplify a Discrete CENP-A Nucleosome Assembly Site on Chromosomes that Drive in Female Meiosis. Curr Biol. 2017;27:2365-2373.e8 pubmed publisher
..We propose that amplified repetitive sequences act as selfish elements by promoting expansion of CENP-A chromatin and increased transmission through the female germline. ..