Bowles R, Patil S, Pincas H, Sealfon S. Validation of efficient high-throughput plasmid and siRNA transfection of human monocyte-derived dendritic cells without cell maturation. J Immunol Methods. 2010;363:21-8 pubmed publisher
..RIG-I knockdown caused a 75% reduction in the induction of IFNÎ² mRNA compared with the negative control siRNA. This protocol should be a valuable tool for probing the immune response pathways activated in human DCs. ..