- Validation of efficient high-throughput plasmid and siRNA transfection of human monocyte-derived dendritic cells without cell maturationRobert Bowles
Center for Translational Systems Biology and Department of Neurology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
J Immunol Methods 363:21-8. 2010..RIG-I knockdown caused a 75% reduction in the induction of IFNβ mRNA compared with the negative control siRNA. This protocol should be a valuable tool for probing the immune response pathways activated in human DCs...