Mohamed Ismail Ashrafali

Summary

Affiliation: Mass. Eye and Ear, Harvard Medical School
Location: Boston, United States
Grants:
Title: Characterization of hepatitis B virus drug resistance in Indian subcontinent patients
Role: Co-Investigator
Year: 2009-2012
Source: Indian Council of Medical Research (ICMR), Ministry of Health and family welfare, Government of India

Title: Development of LigAmp assay for sensitive detection of hepatitis B virus minor variants conferring resistance to antiviral drugs
Role: Principal Investigator
Year: 2010-2012
Source: FLUID Research Grant, Christian Medical College, Vellore, India
Publications:
1: Ismail AM, Lee JS, Dyer DW, Seto D, Rajaiya J, Chodosh J. Selection Pressure
in the Human Adenovirus Fiber Knob Drives Cell Specificity in Epidemic
Keratoconjunctivitis. J Virol. 2016 Nov 1;90(21):9598-9607. PubMed PMID:
.


2: Velay A, Jeulin H, Eschlimann M, Malvé B, Goehringer F, Bensenane M, Frippiat
JP, Abraham P, Ismail AM, Murray JM, Combet C, Zoulim F, Bronowicki JP, Schvoerer
E. Characterization of hepatitis B virus surface antigen variability and impact
on HBs antigen clearance under nucleos(t)ide analogue therapy. J Viral Hepat.
2016 May;23(5):387-98. doi: 10.1111/jvh.12498. Epub 2016 Jan 6. PubMed PMID:
.


3: Singh G, Zhou X, Lee JY, Yousuf MA, Ramke M, Ismail AM, Lee JS, Robinson CM,
Seto D, Dyer DW, Jones MS, Rajaiya J, Chodosh J. Recombination of the epsilon
determinant and corneal tropism: Human adenovirus species D types 15, 29, 56, and
69. Virology. 2015 Nov;485:452-9. doi: 10.1016/j.virol.2015.08.018. Epub 2015 Sep
7. PubMed PMID: ; PubMed Central PMCID: PMC4619159.


4: Ismail AM, Raghavendran A, Sivakumar J, Radhakrishnan M, Rose W, Abraham P.
Mother to child transmission of hepatitis B virus: a cause for concern. Indian J
Med Microbiol. 2015 Feb;33 Suppl:140-3. doi: 10.4103/0255-0857.150931. PubMed
PMID: .


5: Ismail AM, Sachithanandham J, Eapen CE, Kannangai R, Abraham P. Performance of
LigAmp assay for sensitive detection of drug-resistant hepatitis B virus minor
variants in comparison with standard nucleotide sequencing. Mol Diagn Ther. 2014
Dec;18(6):655-63. doi: 10.1007/s40291-014-0119-y. PubMed PMID: .


6: Ismail AM, Puhazhenthi KS, Sivakumar J, Eapen CE, Kannangai R, Abraham P.
Molecular epidemiology and genetic characterization of hepatitis B virus in the
Indian subcontinent. Int J Infect Dis. 2014 Mar;20:1-10. doi:
10.1016/j.ijid.2013.12.007. Epub 2014 Jan 18. PubMed PMID: .


7: Ismail AM, Ramachandran J, Kannangai R, Abraham P. Antiviral efficacy of
adefovir dipivoxil in the treatment of chronic hepatitis B subjects from Indian
subcontinent. Indian J Med Microbiol. 2014 Jan-Mar;32(1):60-3. doi:
10.4103/0255-0857.124312. PubMed PMID: .


8: Ramachandran J, Ismail AM, Chawla G, Fletcher GJ, Goel A, Eapen CE, Abraham P.
Serum HBsAg quantification in treatment-naïve Indian patients with chronic
hepatitis B. Indian J Gastroenterol. 2014 Mar;33(2):131-5. doi:
10.1007/s12664-013-0395-1. Epub 2013 Sep 20. PubMed PMID: .


9: Ismail AM, Samuel P, Ramachandran J, Eapen CE, Kannangai R, Abraham P.
Lamivudine monotherapy in chronic hepatitis B patients from the Indian
subcontinent: antiviral resistance mutations and predictive factors of treatment
response. Mol Diagn Ther. 2014 Feb;18(1):63-71. doi: 10.1007/s40291-013-0054-3.
PubMed PMID: .


10: Ismail AM, Goel A, Kannangai R, Abraham P. Further evidence of hepatitis B
virus genotype I circulation in Northeast India. Infect Genet Evol. 2013
Aug;18:60-5. doi: 10.1016/j.meegid.2013.04.033. Epub 2013 May 9. PubMed PMID:
.


11: Ismail AM, Sharma OP, Kumar MS, Eapen CE, Kannangai R, Abraham P. Virological
response and antiviral resistance mutations in chronic hepatitis B subjects
experiencing entecavir therapy: an Indian subcontinent perspective. Antiviral
Res. 2013 May;98(2):209-16. doi: 10.1016/j.antiviral.2013.02.012. Epub 2013 Feb
26. PubMed PMID: .


12: Ismail AM, Sharma OP, Kumar MS, Kannangai R, Abraham P. Impact of rtI233V
mutation in hepatitis B virus polymerase protein and adefovir efficacy: Homology
modeling and molecular docking studies. Bioinformation. 2013;9(3):121-5. doi:
10.6026/. Epub 2013 Feb 6. PubMed PMID: ; PubMed Central
PMCID: PMC3569598.


13: Ismail AM, Devakumar S, Anantharam R, Fletcher GJ, Subramani T, John GT,
Daniel D, Abraham P. Low frequency of occult hepatitis B infection in anti-HBc
seropositive blood donors: experience from a tertiary care centre in South India.
Blood Transfus. 2012 Apr;10(2):230-2. doi: 10.2450/2011.0046-11. Epub 2011 Nov
15. PubMed PMID: ; PubMed Central PMCID: PMC3320787.


14: Ismail AM, Sivakumar J, Anantharam R, Dayalan S, Samuel P, Fletcher GJ,
Gnanamony M, Abraham P. Performance characteristics and comparison of Abbott and
artus real-time systems for hepatitis B virus DNA quantification. J Clin
Microbiol. 2011 Sep;49(9):3215-21. doi: 10.1128/JCM.00915-11. Epub 2011 Jul 27.
PubMed PMID: ; PubMed Central PMCID: PMC3165604.


15: Fletcher GJ, Samuel P, Christdas J, Gnanamony M, Ismail AM, Anantharam R,
Eapen CE, Chacko MP, Daniel D, Kannangai R, Abraham P. Association of HLA and TNF
polymorphisms with the outcome of HBV infection in the South Indian population.
Genes Immun. 2011 Oct;12(7):552-8. doi: 10.1038/gene.2011.32. Epub 2011 May 19.
PubMed PMID: .


16: Ismail AM, Samuel P, Eapen CE, Kannangai R, Abraham P. Antiviral resistance
mutations and genotype-associated amino acid substitutions in treatment-naïve
hepatitis B virus-infected individuals from the Indian subcontinent.
Intervirology. 2012;55(1):36-44. doi: 10.1159/. Epub 2011 Feb 11. PubMed
PMID: .


17: Fletcher GJ, Gnanamony M, Samuel P, Ismail AM, Kannangai R, Daniel D, Eapen
CE, Abraham P. Association of mannose-binding lectin polymorphisms and HBV
outcome in a South Indian population. Int J Immunogenet. 2010 Jun;37(3):177-84.
doi: 10.1111/j.1744-313X.2010.00908.x. Epub 2010 Feb 24. PubMed PMID: .


18: Fletcher GJ, Gnanamony M, David J, Ismail AM, Subramani T, Abraham P. Do we
need an 'in-house' neutralization assay for confirmation of hepatitis B surface
antigen? Answers from a tertiary care hospital in India. J Gastroenterol Hepatol.
2010 May;25(5):942-5. doi: 10.1111/j.1440-1746.2009.06088.x. Epub 2009 Nov 19.
PubMed PMID: .

Publications

  1. Fletcher G, Gnanamony M, Samuel P, Ismail A, Kannangai R, Daniel D, et al. Association of mannose-binding lectin polymorphisms and HBV outcome in a South Indian population. Int J Immunogenet. 2010;37:177-84 pubmed publisher
    ..Our results provide preliminary evidence that inheritance of codon 52 genotypes and XY/O haplotype associated with low MBL level substantially determine the outcome of HBV infection in a sympatrically isolated South Indian population. ..
  2. Fletcher G, Samuel P, Christdas J, Gnanamony M, Ismail A, Anantharam R, et al. Association of HLA and TNF polymorphisms with the outcome of HBV infection in the South Indian population. Genes Immun. 2011;12:552-8 pubmed publisher
    ..0004). Our study suggests that inheritance of HLA and TNF polymorphisms might explain the outcome of HBV infection in the South Indian population. ..
  3. Ismail A, Sivakumar J, Anantharam R, Dayalan S, Samuel P, Fletcher G, et al. Performance characteristics and comparison of Abbott and artus real-time systems for hepatitis B virus DNA quantification. J Clin Microbiol. 2011;49:3215-21 pubmed publisher
    ..This finding illustrates that the Abbott real-time HBV and artus-DSP systems show more comparable performance than the artus-DB system, meeting the current guidelines for assays to be used in the management of hepatitis B. ..
  4. Ismail A, Sharma O, Kumar M, Kannangai R, Abraham P. Impact of rtI233V mutation in hepatitis B virus polymerase protein and adefovir efficacy: Homology modeling and molecular docking studies. Bioinformation. 2013;9:121-5 pubmed publisher
    ..Therefore, it was predicted that rtI233V substitution may not independently affect the antiviral action of adefovir and incoming dNTP binding. ..
  5. Ramachandran J, Ismail A, Chawla G, Fletcher G, Goel A, Eapen C, et al. Serum HBsAg quantification in treatment-naïve Indian patients with chronic hepatitis B. Indian J Gastroenterol. 2014;33:131-5 pubmed publisher
    ..A single point estimation of qHBsAg in treatment-naïve patients could predict replicative HBeAg-positive CHB, but was not helpful in defining replicative status in the HBeAg-negative CHB. ..
  6. Ismail A, Ramachandran J, Kannangai R, Abraham P. Antiviral efficacy of adefovir dipivoxil in the treatment of chronic hepatitis B subjects from Indian subcontinent. Indian J Med Microbiol. 2014;32:60-3 pubmed publisher
    ..Though the frequencies of adefovir resistance mutations are low, a large majority of subjects showed non-response. Therefore, adefovir in the management of HBV should be used judiciously. ..
  7. Ismail A, Puhazhenthi K, Sivakumar J, Eapen C, Kannangai R, Abraham P. Molecular epidemiology and genetic characterization of hepatitis B virus in the Indian subcontinent. Int J Infect Dis. 2014;20:1-10 pubmed publisher
    ..We have described the distribution of HBV genotypes and subgenotypes and HBsAg subtypes in three major regions of the Indian subcontinent. HBV genetic diversity may play a pivotal role in the clinical outcome of chronic hepatitis B. ..
  8. Ismail A, Sachithanandham J, Eapen C, Kannangai R, Abraham P. Performance of LigAmp assay for sensitive detection of drug-resistant hepatitis B virus minor variants in comparison with standard nucleotide sequencing. Mol Diagn Ther. 2014;18:655-63 pubmed publisher
    ..Therefore, LigAmp has potential clinical utility for appropriate monitoring and tailoring of HBV therapy. ..
  9. Ismail A, Raghavendran A, Sivakumar J, Radhakrishnan M, Rose W, Abraham P. Mother to child transmission of hepatitis B virus: a cause for concern. Indian J Med Microbiol. 2015;33 Suppl:140-3 pubmed publisher
    ..This report illustrates the need for active screening of HBV in pregnant women and implementation of HBV vaccine across all states in India to reduce the burden of disease. ..

More Information

Publications16

  1. Singh G, Zhou X, Lee J, Yousuf M, Ramke M, Ismail A, et al. Recombination of the epsilon determinant and corneal tropism: Human adenovirus species D types 15, 29, 56, and 69. Virology. 2015;485:452-9 pubmed publisher
    ..However, HAdV-D56 entry into human corneal epithelial cells and fibroblasts in vitro dramatically exceeded that of the other three viruses. We conclude that the hexon epsilon determinant is not a prime contributor to corneal tropism. ..
  2. Fletcher G, Gnanamony M, David J, Ismail A, Subramani T, Abraham P. Do we need an 'in-house' neutralization assay for confirmation of hepatitis B surface antigen? Answers from a tertiary care hospital in India. J Gastroenterol Hepatol. 2010;25:942-5 pubmed publisher
    ..This technique is easily adaptable, cost-effective and reliable for the confirmation of HBsAg in a low resource setting, enhancing the overall quality of HBsAg screening. ..
  3. Ismail A, Samuel P, Eapen C, Kannangai R, Abraham P. Antiviral resistance mutations and genotype-associated amino acid substitutions in treatment-naïve hepatitis B virus-infected individuals from the Indian subcontinent. Intervirology. 2012;55:36-44 pubmed publisher
    ..This data shows that antiviral-resistant HBV strains do exist in treatment-naïve individuals in this region. Further studies are essential to characterize the role of HBVrt amino acid substitutions in response to anti-HBV therapy. ..
  4. Ismail A, Devakumar S, Anantharam R, Fletcher G, Subramani T, John G, et al. Low frequency of occult hepatitis B infection in anti-HBc seropositive blood donors: experience from a tertiary care centre in South India. Blood Transfus. 2012;10:230-2 pubmed publisher
  5. Ismail A, Sharma O, Kumar M, Eapen C, Kannangai R, Abraham P. Virological response and antiviral resistance mutations in chronic hepatitis B subjects experiencing entecavir therapy: an Indian subcontinent perspective. Antiviral Res. 2013;98:209-16 pubmed publisher
    ..In addition, none of the subjects developed antiviral resistance mutations to entecavir. Therefore entecavir is a suitable drug of choice in the management of chronic HBV. ..
  6. Ismail A, Samuel P, Ramachandran J, Eapen C, Kannangai R, Abraham P. Lamivudine monotherapy in chronic hepatitis B patients from the Indian subcontinent: antiviral resistance mutations and predictive factors of treatment response. Mol Diagn Ther. 2014;18:63-71 pubmed publisher
    ..037). Considering the limited potency and high resistance rates to lamivudine therapy, our study emphasizes the use of more potent drugs in the management of chronic hepatitis B in the Indian subcontinent. ..
  7. Pan H, Yan Y, Zhang J, Zhao S, Feng L, Ou J, et al. Rapid Construction of a Replication-Competent Infectious Clone of Human Adenovirus Type 14 by Gibson Assembly. Viruses. 2018;10: pubmed publisher