Helen J Cooper

Summary

Affiliation: University of Birmingham
Country: UK

Publications

  1. ncbi request reprint The role of electron capture dissociation in biomolecular analysis
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    Mass Spectrom Rev 24:201-22. 2005
  2. ncbi request reprint Identification of sites of ubiquitination in proteins: a fourier transform ion cyclotron resonance mass spectrometry approach
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Anal Chem 76:6982-8. 2004
  3. ncbi request reprint Data-dependent electron capture dissociation FT-ICR mass spectrometry for proteomic analyses
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Proteome Res 4:1538-44. 2005
  4. pmc The effect of phosphorylation on the electron capture dissociation of peptide ions
    Andrew J Creese
    School of Biosciences, University of Birmingham, UK Birmingham, United Kingdom
    J Am Soc Mass Spectrom 19:1263-74. 2008
  5. pmc To What Extent is FAIMS Beneficial in the Analysis of Proteins?
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    J Am Soc Mass Spectrom 27:566-77. 2016
  6. doi request reprint Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots
    Rian L Griffiths
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Analyst 140:6879-85. 2015
  7. pmc Liquid chromatography electron capture dissociation tandem mass spectrometry (LC-ECD-MS/MS) versus liquid chromatography collision-induced dissociation tandem mass spectrometry (LC-CID-MS/MS) for the identification of proteins
    Andrew J Creese
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    J Am Soc Mass Spectrom 18:891-7. 2007
  8. ncbi request reprint Fourier transform ion cyclotron resonance mass spectrometry for the analysis of small ubiquitin-like modifier (SUMO) modification: identification of lysines in RanBP2 and SUMO targeted for modification during the E3 autoSUMOylation reaction
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Anal Chem 77:6310-9. 2005
  9. ncbi request reprint Investigation of the presence of b ions in electron capture dissociation mass spectra
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, UK
    J Am Soc Mass Spectrom 16:1932-40. 2005
  10. pmc Differential phosphoproteomics of fibroblast growth factor signaling: identification of Src family kinase-mediated phosphorylation events
    Debbie L Cunningham
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    J Proteome Res 9:2317-28. 2010

Collaborators

Detail Information

Publications64

  1. ncbi request reprint The role of electron capture dissociation in biomolecular analysis
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    Mass Spectrom Rev 24:201-22. 2005
    ..The application of ECD to the analysis of polymers, peptide nucleic acids, and oligonucleotides is also discussed...
  2. ncbi request reprint Identification of sites of ubiquitination in proteins: a fourier transform ion cyclotron resonance mass spectrometry approach
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Anal Chem 76:6982-8. 2004
    ..Assignments were confirmed by AI ECD or IRMPD. The Gly-Gly modification is stable and both tandem mass spectrometric techniques are suitable, providing extensive sequence coverage and retention of the modification on backbone fragments...
  3. ncbi request reprint Data-dependent electron capture dissociation FT-ICR mass spectrometry for proteomic analyses
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Proteome Res 4:1538-44. 2005
    ..Protein ROR2 was assigned, as was IgG (Fc domain). The results demonstrate the suitability of ECD as an integral technique in high-throughput proteomic strategies...
  4. pmc The effect of phosphorylation on the electron capture dissociation of peptide ions
    Andrew J Creese
    School of Biosciences, University of Birmingham, UK Birmingham, United Kingdom
    J Am Soc Mass Spectrom 19:1263-74. 2008
    ..Alternatively, improved sequence coverage can be achieved by performing ECD on triply-charged precursor ions. The findings are important for the understanding of gas-phase fragmentation of phosphopeptides...
  5. pmc To What Extent is FAIMS Beneficial in the Analysis of Proteins?
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    J Am Soc Mass Spectrom 27:566-77. 2016
    ..Similarly, the application of FAIMS in proteomics and targeted analysis of peptides are considered...
  6. doi request reprint Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots
    Rian L Griffiths
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Analyst 140:6879-85. 2015
    ..The work has implications for multiplexed clinical assays of multiple analytes. ..
  7. pmc Liquid chromatography electron capture dissociation tandem mass spectrometry (LC-ECD-MS/MS) versus liquid chromatography collision-induced dissociation tandem mass spectrometry (LC-CID-MS/MS) for the identification of proteins
    Andrew J Creese
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    J Am Soc Mass Spectrom 18:891-7. 2007
    ..The results show that although protein coverage was lower for LC-ECD-MS/MS than for LC-CID-MS/MS, LC-ECD-MS/MS resulted in longer peptide sequence tags, providing greater confidence in protein assignment...
  8. ncbi request reprint Fourier transform ion cyclotron resonance mass spectrometry for the analysis of small ubiquitin-like modifier (SUMO) modification: identification of lysines in RanBP2 and SUMO targeted for modification during the E3 autoSUMOylation reaction
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Anal Chem 77:6310-9. 2005
    ....
  9. ncbi request reprint Investigation of the presence of b ions in electron capture dissociation mass spectra
    Helen J Cooper
    School of Biosciences, University of Birmingham, Edgbaston, UK
    J Am Soc Mass Spectrom 16:1932-40. 2005
    ..The results suggest that the presence of b ions in ECD spectra is a consequence of both charge carrier and peptide structure. Possible mechanisms for the formation of b ions following electron capture are discussed...
  10. pmc Differential phosphoproteomics of fibroblast growth factor signaling: identification of Src family kinase-mediated phosphorylation events
    Debbie L Cunningham
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    J Proteome Res 9:2317-28. 2010
    ..We further identify 18 SFK-dependent Dok1 interactions and 9 SFK-dependent Dok1 phosphorylation sites, 6 of which had not previously been known to be SFK-dependent...
  11. pmc Top-down mass analysis of protein tyrosine nitration: comparison of electron capture dissociation with "slow-heating" tandem mass spectrometry methods
    Victor A Mikhailov
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Anal Chem 82:7283-92. 2010
    ..We conclude that ECD can be used in the top-down analysis of nitrated proteins, but precise localization of the sites of nitration may require either of the "slow-heating" methods...
  12. doi request reprint On-line liquid chromatography electron capture dissociation for the characterization of phosphorylation sites in proteins
    Steve M M Sweet
    University of Birmingham, School of Biosciences, Birmingham, UK
    Methods Mol Biol 527:191-9, x. 2009
    ..The following methods are designed to focus ECD events on phosphopeptides within a complex peptide sample, either by using phosphoric acid neutral loss peaks as a trigger or by targeted analysis of predetermined precursor masses...
  13. doi request reprint SLoMo: automated site localization of modifications from ETD/ECD mass spectra
    Christopher M Bailey
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Proteome Res 8:1965-71. 2009
    ..SLoMo accepts input data from a variety of formats (e.g., Sequest, OMSSA). We validate SLoMo with high and low resolution ETD, ECD, and CID data...
  14. doi request reprint Targeted online liquid chromatography electron capture dissociation mass spectrometry for the localization of sites of in vivo phosphorylation in human Sprouty2
    Steve M M Sweet
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Anal Chem 80:6650-7. 2008
    ..The approach was applied to phosphorylation analysis of human Sprouty2, a regulator of receptor tyrosine kinase signaling. Fifteen sites of phosphorylation were identified, 11 of which are novel...
  15. pmc Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry
    Steve M M Sweet
    Cancer Research UK Growth Factor Group, College of Life and Environmental Sciences, Universityof Birmingham, Edgbaston, Birmingham B152TT, United Kingdom
    Mol Cell Proteomics 8:904-12. 2009
    ..The combination of confident CID and ECD identification and confident CID and ECD localization is particularly valuable in cases where a phosphopeptide is identified just once within a phosphoproteomics experiment...
  16. doi request reprint Electron capture dissociation mass spectrometry of tyrosine nitrated peptides
    Andrew W Jones
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    J Am Soc Mass Spectrom 21:268-77. 2010
    ..The origin of the neutral losses has been investigated by use of activated ion (AI) ECD. Loss of ammonia appears to be the result of non-covalent interactions between the nitro group and protonated lysine side-chains...
  17. doi request reprint Liquid Extraction Surface Analysis Mass Spectrometry Coupled with Field Asymmetric Waveform Ion Mobility Spectrometry for Analysis of Intact Proteins from Biological Substrates
    Joscelyn Sarsby
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Anal Chem 87:6794-800. 2015
    ..In addition, FAIMS enables gas-phase separation of molecular classes, for example, lipids and proteins, enabling improved analysis of both sets of species from a single LESA extraction. ..
  18. pmc Probing the complementarity of FAIMS and strong cation exchange chromatography in shotgun proteomics
    Andrew J Creese
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
    J Am Soc Mass Spectrom 24:431-43. 2013
    ..The lack of overlap between FAIMS and SCX highlights the complementarity of the two techniques. Charge state analysis of the peptide assignments showed that the FAIMS approach identified a much greater proportion of triply-charged ions...
  19. pmc Activated Ion Electron Capture Dissociation (AI ECD) of proteins: synchronization of infrared and electron irradiation with ion magnetron motion
    Victor A Mikhailov
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    J Am Soc Mass Spectrom 20:763-71. 2009
    ....
  20. ncbi request reprint Strategy for the identification of sites of phosphorylation in proteins: neutral loss triggered electron capture dissociation
    Steve M M Sweet
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Anal Chem 78:7563-9. 2006
    ..A further benefit of the method is that overall protein sequence coverage is improved. Sequence information from nonphosphorylated peptides is obtained as a result of the CID step...
  21. pmc Large-scale analysis of peptide sequence variants: the case for high-field asymmetric waveform ion mobility spectrometry
    Andrew J Creese
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, UK
    Anal Chem 85:4836-43. 2013
    ..The FAIMS approach identified 35% of the peptide library, whereas LC-MS/MS alone identified 8% and LC-MS/MS with strong cation exchange chromatography prefractionation identified 17.3% of the library...
  22. pmc Database search strategies for proteomic data sets generated by electron capture dissociation mass spectrometry
    Steve M M Sweet
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Proteome Res 8:5475-84. 2009
    ..We compare our presearch processing algorithm with a similar algorithm recently developed for electron transfer dissociation (ETD) data...
  23. doi request reprint Higher energy collision dissociation (HCD) product ion-triggered electron transfer dissociation (ETD) mass spectrometry for the analysis of N-linked glycoproteins
    Charandeep Singh
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    J Proteome Res 11:4517-25. 2012
    ..The results show that the product ion-triggered approach shows promise for the field of glycoproteomics and highlight the requirement for more sophisticated data mining tools...
  24. ncbi request reprint Protein partners in the life history of activated fibroblast growth factor receptors
    Anna Vecchione
    CR UK Growth Factor Group, School of Biosciences, University of Birmingham, Birmingham, UK
    Proteomics 7:4565-78. 2007
    ..The strategic approach adopted in this study has revealed bona fide novel effectors of the FGFR signaling pathway...
  25. doi request reprint LESA FAIMS Mass Spectrometry for the Spatial Profiling of Proteins from Tissue
    Rian L Griffiths
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, U K
    Anal Chem 88:6758-66. 2016
    ....
  26. doi request reprint FAIMS and Phosphoproteomics of Fibroblast Growth Factor Signaling: Enhanced Identification of Multiply Phosphorylated Peptides
    Hongyan Zhao
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Proteome Res 14:5077-87. 2015
    ..In addition to providing a compelling example of the complementarity of FAIMS in phosphoproteomics, the results provide a valuable resource of phosphorylation sites for further investigation of FGF signaling and trafficking. ..
  27. ncbi request reprint Electron capture dissociation in the analysis of protein phosphorylation
    Steve M M Sweet
    University of Birmingham, School of Biosciences, Edgbaston, Birmingham, UK
    Expert Rev Proteomics 4:149-59. 2007
    ..Electron capture dissociation has inherent advantages for phosphorylation analysis. The use of electron capture dissociation in this area to date is reviewed and future prospects are outlined...
  28. pmc Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides
    Andrew W Jones
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Phys Chem Chem Phys 12:13394-9. 2010
    ....
  29. doi request reprint The radical ion chemistry of S-nitrosylated peptides
    Andrew W Jones
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    J Am Soc Mass Spectrom 23:2063-74. 2012
    ....
  30. doi request reprint Top-down proteomics and direct surface sampling of neonatal dried blood spots: diagnosis of unknown hemoglobin variants
    Rebecca L Edwards
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    J Am Soc Mass Spectrom 23:1921-30. 2012
    ..To address that issue, we have compared manual data analysis with freely available data analysis software (ProsightPTM). The results demonstrate the power of top-down proteomics for hemoglobin variant analysis in newborn samples...
  31. doi request reprint Dissociation techniques in mass spectrometry-based proteomics
    Andrew W Jones
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Analyst 136:3419-29. 2011
    ..Here, we describe each of these techniques and their use in proteomics. The principles, advantages, limitations, and applications are discussed...
  32. doi request reprint Electron induced dissociation: a mass spectrometry technique for the structural analysis of trinuclear oxo-centred carboxylate-bridged iron complexes
    Malgorzata A Kaczorowska
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    J Am Soc Mass Spectrom 21:1398-403. 2010
    ..The results suggest that EID is particularly suitable for the structural analysis of singly-charged polynuclear metal complexes...
  33. pmc Proteomic analysis of a noninvasive human model of acute inflammation and its resolution: the twenty-one day gingivitis model
    Melissa M Grant
    Periodontal Research Group, School of Dentistry, College of Medical and Dental Sciences, University of Birmingham, St Chads Queensway, Birmingham, B4 6NN, United Kingdom
    J Proteome Res 9:4732-44. 2010
    ..PolySNAP3 clustering software was used in a multilayered analytical approach. Clusters of proteins that associated with changes to the clinical parameters included neuronal and synapse associated proteins...
  34. ncbi request reprint Dynamic range and mass accuracy of wide-scan direct infusion nanoelectrospray fourier transform ion cyclotron resonance mass spectrometry-based metabolomics increased by the spectral stitching method
    Andrew D Southam
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Anal Chem 79:4595-602. 2007
    ..18 ppm and detected over 3000 peaks in liver extract. This novel approach increases metabolome coverage, has very high mass accuracy, and at 5.5 min/sample is conducive for high-throughput metabolomics...
  35. pmc Native Liquid Extraction Surface Analysis Mass Spectrometry: Analysis of Noncovalent Protein Complexes Directly from Dried Substrates
    Nicholas J Martin
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    J Am Soc Mass Spectrom 26:1320-7. 2015
    ..Heme-bound dimers and monomers were also observed. The 'contact' LESA approach was particularly suitable for the analysis of hemoglobin tetramers from DBS...
  36. pmc Electron capture dissociation and collision-induced dissociation of metal ion (Ag(+), Cu(2+), Zn(2+), Fe(2+), and Fe(3+)) complexes of polyamidoamine (PAMAM) dendrimers
    Malgorzata A Kaczorowska
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    J Am Soc Mass Spectrom 20:674-81. 2009
    ..The Zn(2+) and Fe(2+) complexes do not appear to involve coordination by the dendrimer core...
  37. doi request reprint Electron induced dissociation (EID) tandem mass spectrometry of octaethylporphyrin and its iron(III) complex
    Malgorzata A Kaczorowska
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    Chem Commun (Camb) 47:418-20. 2011
    ..Singly-charged fragments are also observed. EID fragmentation differs significantly to that observed in electron ionisation mass spectrometry...
  38. pmc Dried blood spot proteomics: surface extraction of endogenous proteins coupled with automated sample preparation and mass spectrometry analysis
    Nicholas J Martin
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    J Am Soc Mass Spectrom 24:1242-9. 2013
    ..The proteins identified cross a concentration range of four orders of magnitude. The method is evaluated and the results discussed in terms of the proteins identified and their potential use as biomarkers in screening programs. ..
  39. pmc Separation and identification of isomeric glycopeptides by high field asymmetric waveform ion mobility spectrometry
    Andrew J Creese
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom
    Anal Chem 84:2597-601. 2012
    ..The results suggest that glycan position plays a key role in determining gas-phase glycopeptide structure and have implications for the application of FAIMS in glycoproteomics...
  40. doi request reprint Compound heterozygotes and beta-thalassemia: top-down mass spectrometry for detection of hemoglobinopathies
    Rebecca L Edwards
    School of Biosciences, University of Birmingham, Birmingham, UK
    Proteomics 14:1232-8. 2014
    ..Manual analysis of the mass spectra revealed that, in all cases, the variants may be confirmed. Direct surface sampling MS should be considered as an alternative to current screening techniques for the diagnosis of Hb variants. ..
  41. doi request reprint Electron capture dissociation mass spectrometry of metallo-supramolecular complexes
    Malgorzata A Kaczorowska
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    J Am Soc Mass Spectrom 21:300-9. 2010
    ..The results suggest that iron-bound dimers may be of the form Fe(I)(2)L(2)(2+) and that ECD by metallo-complexes allows access, in the gas phase, to oxidation states and coordination chemistry that cannot be accessed in solution...
  42. doi request reprint Characterization of polyphosphoesters by Fourier transform ion cyclotron resonance mass spectrometry
    Malgorzata A Kaczorowska
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, United Kingdom
    J Am Soc Mass Spectrom 20:2238-47. 2009
    ....
  43. pmc Comprehensive mapping of O-glycosylation in flagellin from Campylobacter jejuni 11168: A multienzyme differential ion mobility mass spectrometry approach
    Gloria N Ulasi
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    Proteomics 15:2733-45. 2015
    ..These results demonstrate the usefulness of differential ion mobility in proteomics investigations of PTMs. ..
  44. pmc Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groups
    Malgorzata A Kaczorowska
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    J Am Soc Mass Spectrom 19:1312-9. 2008
    ..The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers...
  45. pmc Top-down and bottom-up identification of proteins by liquid extraction surface analysis mass spectrometry of healthy and diseased human liver tissue
    Joscelyn Sarsby
    Physical Sciences of Imaging in the Biomedical Sciences doctoral training centre, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    J Am Soc Mass Spectrom 25:1953-61. 2014
    ..The reliable identification of FABP1 and its variant by top-down LESA MS suggests that the approach may be suitable for imaging NASH pathology in sections from liver biopsies. ..
  46. doi request reprint Plasma levels of complement 4a protein are increased in Alzheimer's disease
    Stuart Bennett
    School of Sport and Exercise Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Alzheimer Dis Assoc Disord 26:329-34. 2012
    ..These data suggest that inflammatory processes, which have been shown to be involved in AD pathology in the brain, are also present in plasma...
  47. doi request reprint Hemoglobin variant analysis of whole blood and dried blood spots by MS
    Rebecca L Edwards
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Bioanalysis 5:2043-52. 2013
    ..MS approaches that are suitable for population screening are discussed, as are recent advances in direct surface analysis of dried blood spots. ..
  48. pmc Separation of cis and trans Isomers of Polyproline by FAIMS Mass Spectrometry
    Andrew J Creese
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    J Am Soc Mass Spectrom 27:2071-2074. 2016
    ..Differentiation of these conformers may be achieved both through use of a cylindrical FAIMS device and a miniaturized ultrahigh field planar FAIMS device. Graphical Abstract ᅟ...
  49. doi request reprint Online LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins
    Hongyan Zhao
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Methods Mol Biol 1355:241-50. 2016
    ..Here, we describe the protocol for large-scale phosphorylation analysis by LC-FAIMS-MS/MS. ..
  50. doi request reprint Direct Tissue Profiling of Protein Complexes: Toward Native Mass Spectrometry Imaging
    Rian L Griffiths
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Anal Chem 88:606-9. 2016
    ..Here, we show that the intact hemoglobin complex can be sampled directly from thin tissue sections of mouse liver and correlated to a visible vascular feature, paving the way for native mass spectrometry imaging. ..
  51. pmc Hemoglobin variant analysis via direct surface sampling of dried blood spots coupled with high-resolution mass spectrometry
    Rebecca L Edwards
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Anal Chem 83:2265-70. 2011
    ..The method allows for repeated analysis of a single blood spot over a prolonged time period and is tolerant of blood spot storage conditions...
  52. pmc Novel glycosylation sites localized in Campylobacter jejuni flagellin FlaA by liquid chromatography electron capture dissociation tandem mass spectrometry
    Cleidiane G Zampronio
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, U K
    J Proteome Res 10:1238-45. 2011
    ..For comparison, on-line liquid chromatography collision-induced dissociation of the tryptic digests was performed, but it was not possible to assign sites of glycosylation by that method...
  53. doi request reprint Challenges and opportunities in mass spectrometric analysis of proteins from dried blood spots
    Nicholas J Martin
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Expert Rev Proteomics 11:685-95. 2014
    ..This article focuses on the progress made in the mass spectrometric analysis of proteins from DBS and discusses the benefits and challenges facing this emerging field. ..
  54. doi request reprint Direct analysis of intact proteins from Escherichia coli colonies by liquid extraction surface analysis mass spectrometry
    Elizabeth C Randall
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    Anal Chem 86:10504-10. 2014
    ..Moreover, the results show that the "contact" LESA approach results in a smaller sampling area than typical LESA, which may have implications for spatial profiling...
  55. pmc Can museum egg specimens be used for proteomic analyses?
    Steven J Portugal
    The Centre for Ornithology, School of Biosciences, College of Life and Environmental Sciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Proteome Sci 8:40. 2010
    ..The determination of the proteins was carried out using enzymatic cleavage followed by high-performance mass spectrometry...
  56. pmc De novo design of Ln(III) coiled coils for imaging applications
    Matthew R Berwick
    School of Chemistry and School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, United Kingdom
    J Am Chem Soc 136:1166-9. 2014
    ..Despite this the gadolinium coiled coil, the first to be reported, displays promising magnetic resonance contrast capabilities. ..
  57. ncbi request reprint Characterization of amino acid side chain losses in electron capture dissociation
    Helen J Cooper
    Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Florida State University, Tallahassee 32310, USA
    J Am Soc Mass Spectrom 13:241-9. 2002
    ..Losses from arginine, histidine, and asparigine/glutamine can be used to ascertain their presence, as in the analysis of unknown peptides, particularly those with non-linear structures...
  58. ncbi request reprint Determination of aberrant O-glycosylation in the IgA1 hinge region by electron capture dissociation fourier transform-ion cyclotron resonance mass spectrometry
    Matthew B Renfrow
    National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida 32310 4005, USA
    J Biol Chem 280:19136-45. 2005
    ....
  59. ncbi request reprint Determination of the activation energy for unimolecular dissociation of a non-covalent gas-phase peptide: substrate complex by infrared multiphoton dissociation fourier transform ion cyclotron resonance mass spectrometry
    Mathias Schäfer
    Institute for Organic Chemistry, University Cologne, Koln, Germany
    J Am Soc Mass Spectrom 14:1282-9. 2003
    ..We obtain EaI(laser) = 0.67 eV (Dunbar approximation) and EaII(laser) = 1.12 eV (Paech et al. approximation). Differences between the two approaches are discussed with respect to non-covalent complexes...
  60. ncbi request reprint Characterization of the P13 membrane protein of Borrelia burgdorferi by mass spectrometry
    Carol L Nilsson
    Institute of Medical Biochemistry, Goteborg University, Sweden
    J Am Soc Mass Spectrom 13:295-9. 2002
    ..New structural information for this membrane protein was provided by peptide mapping with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) and sequencing with ESI-quadrupole-TOF tandem MS...
  61. pmc Specific electrochemical iodination of horse heart myoglobin at tyrosine 103 as determined by Fourier transform ion cyclotron resonance mass spectrometry
    Jesus Iniesta
    Centre for Molecular and Biomedical Science, Faculty of Health and Life Sciences, Coventry University, Coventry CV1 5FB, UK
    Arch Biochem Biophys 474:1-7. 2008
    ..More extensive sequence coverage was obtained with electron capture dissociation (ECD), allowing unambiguous assignment of the site of iodination to Tyr103...
  62. ncbi request reprint Mass spectrometry in demonstrating the site-specific nitration of hen egg white lysozyme by an improved electrochemical method
    Dominic Matters
    Centre for Molecular and Biomedical Sciences, Faculty of Health and Life Sciences, Coventry University, Coventry CV1 5FB, UK
    Anal Biochem 356:171-81. 2006
    ..Matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization time-of-flight analysis of the tryptic peptides of mononitrated lysozyme identified the site of nitration as Tyr23...
  63. ncbi request reprint Letter: the diagnostic value of amino acid side-chain losses in electron capture dissociation of polypeptides. Comment on: "Can the (M(.)-X) region in electron capture dissociation provide reliable information on amino acid composition of polypeptide
    Helen J Cooper
    Eur J Mass Spectrom (Chichester, Eng) 9:221-2. 2003
  64. ncbi request reprint Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometric analysis of metal-ion selected dynamic protein libraries
    Helen J Cooper
    Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, Tallahassee, Florida 32310 3706, USA
    J Am Chem Soc 125:5331-9. 2003
    ..The experiments also suggest the presence of secondary structure in the gas phase, possibly due to partial retention of the solution-phase coiled coil structure...