Alan J Williams

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. ncbi request reprint The interaction of ryanoids with individual ryanodine receptor channels
    Alan J Williams
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, Dovehouse St, London SW3 6LY, UK
    Biol Res 37:527-38. 2004
  2. pmc Voltage-sensitive equilibrium between two states within a ryanoid-modified conductance state of the ryanodine receptor channel
    Bhavna Tanna
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, UK
    Biophys J 88:2585-96. 2005
  3. ncbi request reprint Quantification of the effects of a ryanodine receptor channel mutation on interaction with a ryanoid
    Kishani M Ranatunga
    Cardiac Medicine, National Heart and Lung Institute, Imperial College London, London, UK
    Mol Membr Biol 24:185-93. 2007
  4. pmc Changes in negative charge at the luminal mouth of the pore alter ion handling and gating in the cardiac ryanodine-receptor
    Fiona C Mead-Savery
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London SW3 6LY, United Kingdom
    Biophys J 96:1374-87. 2009
  5. ncbi request reprint The interaction of an impermeant cation with the sheep cardiac RyR channel alters ryanoid association
    Bhavna Tanna
    Cardiac Medicine, National Heart and Lung Institute, Imperial College London, Guy Scadding Building, Dovehouse Street, London SW3 6LY, UK
    Mol Pharmacol 69:1990-7. 2006
  6. pmc An anionic ryanoid, 10-O-succinoylryanodol, provides insights into the mechanisms governing the interaction of ryanoids and the subsequent altered function of ryanodine-receptor channels
    Bhavna Tanna
    Cardiac Medicine, National Heart and Lung Institute, Imperial College of Science, Technology, and Medicine, London SW3 6LY, UK
    J Gen Physiol 121:551-61. 2003
  7. pmc Ryanodine-induced structural alterations in the RyR channel suggested by neomycin block
    Fiona Mead
    Department of Cardiac Medicine, National Heart and Lung Institute, Imperial College of Science, Technology and Medicine, London SW3 6LY, United Kingdom
    Biophys J 82:1964-74. 2002
  8. ncbi request reprint The Gln4863Ala mutation within a putative, pore-lining trans-membrane helix of the cardiac ryanodine receptor channel alters both the kinetics of ryanoid interaction and the subsequent fractional conductance
    Kishani M Ranatunga
    Myocardial Systems Biology Group, National Heart and Lung Institute, Guy Scadding Building, Imperial College London, London SW3 6LY, United Kingdom
    Mol Pharmacol 68:840-6. 2005
  9. ncbi request reprint Excess noise in modified conductance states following the interaction of ryanoids with cardiac ryanodine receptor channels
    Bhavna Tanna
    Cardiac Medicine, National Heart and Lung Institute, Imperial College of Science, Technology and Medicine, Dovehouse Street, SW3 6LY, London, UK
    FEBS Lett 516:35-9. 2002
  10. ncbi request reprint A novel and rapid approach to isolating functional ryanodine receptors
    Duncan J West
    Department of Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Dovehouse Street, London SW3 6LY, UK
    Biochem Biophys Res Commun 294:402-7. 2002

Collaborators

Detail Information

Publications26

  1. ncbi request reprint The interaction of ryanoids with individual ryanodine receptor channels
    Alan J Williams
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, Dovehouse St, London SW3 6LY, UK
    Biol Res 37:527-38. 2004
    ..The probability of occurrence of the RyR-ryanoid complex is sensitive to trans-membrane voltage, with the vast majority of the influence of potential arising from a voltage-driven alteration in the affinity of the ryanoid-binding site...
  2. pmc Voltage-sensitive equilibrium between two states within a ryanoid-modified conductance state of the ryanodine receptor channel
    Bhavna Tanna
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, UK
    Biophys J 88:2585-96. 2005
    ..However, the mechanisms determining this equilibrium differ from those responsible for the voltage-sensitive equilibrium between high- and low-affinity forms of the receptor...
  3. ncbi request reprint Quantification of the effects of a ryanodine receptor channel mutation on interaction with a ryanoid
    Kishani M Ranatunga
    Cardiac Medicine, National Heart and Lung Institute, Imperial College London, London, UK
    Mol Membr Biol 24:185-93. 2007
    ..We infer that Q4863 is not involved with the voltage-drop but is able to influence ryanoid-bound structural changes. We discuss structural mechanisms by which this mutation could affect ryanoid interaction...
  4. pmc Changes in negative charge at the luminal mouth of the pore alter ion handling and gating in the cardiac ryanodine-receptor
    Fiona C Mead-Savery
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London SW3 6LY, United Kingdom
    Biophys J 96:1374-87. 2009
    ....
  5. ncbi request reprint The interaction of an impermeant cation with the sheep cardiac RyR channel alters ryanoid association
    Bhavna Tanna
    Cardiac Medicine, National Heart and Lung Institute, Imperial College London, Guy Scadding Building, Dovehouse Street, London SW3 6LY, UK
    Mol Pharmacol 69:1990-7. 2006
    ..These actions involve binding of TEA(+) to different, but weakly interacting, sites in the RyR channel...
  6. pmc An anionic ryanoid, 10-O-succinoylryanodol, provides insights into the mechanisms governing the interaction of ryanoids and the subsequent altered function of ryanodine-receptor channels
    Bhavna Tanna
    Cardiac Medicine, National Heart and Lung Institute, Imperial College of Science, Technology, and Medicine, London SW3 6LY, UK
    J Gen Physiol 121:551-61. 2003
    ..These investigations also demonstrate that the charge of the ryanoid has a major influence on the rate of association of the ligand with its receptor indicating that ionic interactions are likely to be involved in this reaction...
  7. pmc Ryanodine-induced structural alterations in the RyR channel suggested by neomycin block
    Fiona Mead
    Department of Cardiac Medicine, National Heart and Lung Institute, Imperial College of Science, Technology and Medicine, London SW3 6LY, United Kingdom
    Biophys J 82:1964-74. 2002
    ..This suggests that ryanodine induces changes at the luminal mouth of the channel and may confer increased rigidity to the channel protein...
  8. ncbi request reprint The Gln4863Ala mutation within a putative, pore-lining trans-membrane helix of the cardiac ryanodine receptor channel alters both the kinetics of ryanoid interaction and the subsequent fractional conductance
    Kishani M Ranatunga
    Myocardial Systems Biology Group, National Heart and Lung Institute, Guy Scadding Building, Imperial College London, London SW3 6LY, United Kingdom
    Mol Pharmacol 68:840-6. 2005
    ..We suggest that replacement of a glutamine by an alanine residue at position 4863 causes RyR2 to simultaneously alter interactions with both ends of the ryanoid molecule...
  9. ncbi request reprint Excess noise in modified conductance states following the interaction of ryanoids with cardiac ryanodine receptor channels
    Bhavna Tanna
    Cardiac Medicine, National Heart and Lung Institute, Imperial College of Science, Technology and Medicine, Dovehouse Street, SW3 6LY, London, UK
    FEBS Lett 516:35-9. 2002
    ..Following the interaction of certain ryanodine derivatives, RyR channels undergo transitions between two conformations with slightly different ion-handling properties...
  10. ncbi request reprint A novel and rapid approach to isolating functional ryanodine receptors
    Duncan J West
    Department of Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Dovehouse Street, London SW3 6LY, UK
    Biochem Biophys Res Commun 294:402-7. 2002
    ..RyRs isolated by this method display characteristic functional behaviour as assessed by radioligand binding and single channel analyses...
  11. ncbi request reprint Removal of clustered positive charge from dihydropyridine receptor II-III loop peptide augments activation of ryanodine receptors
    Mark L Bannister
    Department of Cardiac Medicine, National Heart and Lung Institute, Imperial College London, Dovehouse Street, SW3 6LY, London, UK
    Biochem Biophys Res Commun 314:667-74. 2004
    ....
  12. ncbi request reprint Activation of the sheep cardiac Ca2+ release channel by simple heteroaromatics
    Mark L Bannister
    Department of Cardiac Medicine, National Heart and Lung Institute, Imperial College London, Dovehouse Street, London SW3 6LY, UK
    Biochem Biophys Res Commun 317:397-400. 2004
    ..The propensity to activate the channel requires notably little chemical functionality and is associated with the capacity for charge-transfer complex formation in conjunction with steric bulk...
  13. pmc Electrostatic mechanisms underlie neomycin block of the cardiac ryanodine receptor channel (RyR2)
    Fiona C Mead
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London, United Kingdom
    Biophys J 87:3814-25. 2004
    ..These observations emphasize the usefulness of neomycin as a probe for regions of charge in both the cytosolic and luminal mouths of the RyR2 pore...
  14. doi request reprint Myocardial stunning is associated with impaired calcium uptake by sarcoplasmic reticulum
    Sanjay Kumar
    National Heart and Lung Institute, Imperial College, London, UK
    Biochem Biophys Res Commun 387:77-82. 2009
    ..3+/-13.5 vs. 73.0+/-15.6 nmol/min/mg protein, p=0.03). The ability of SR calcium ATPase to sequester cytosolic calcium is impaired in stunned myocardium. This is a potential mechanism underlying contractile dysfunction...
  15. doi request reprint The mechanism of flecainide action in CPVT does not involve a direct effect on RyR2
    Mark L Bannister
    From the Institute of Molecular and Experimental Medicine, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff, United Kingdom M L B, N L T, S M, C M, C H G, A J W and Myocardial Function Section, National Heart and Lung Institute, Imperial College London, London, United Kingdom M B S, K T M
    Circ Res 116:1324-35. 2015
    ..However, there is presently no direct evidence to support the notion that flecainide blocks RyR2 Ca(2+) flux in the physiologically relevant (luminal-to-cytoplasmic) direction. The mechanism of flecainide action remains controversial...
  16. pmc Flecainide reduces Ca(2+) spark and wave frequency via inhibition of the sarcolemmal sodium current
    Markus B Sikkel
    Myocardial Function Section, National Heart and Lung Institute, Fourth Floor, Imperial Centre for Translational and Experimental Medicine, Imperial College, Hammersmith Campus, Du Cane Road, London W12 0NN, UK
    Cardiovasc Res 98:286-96. 2013
    ..There have been conflicting results regarding whether flecainide reduces Ca(2+) waves in isolated cardiomyocytes. We sought to confirm whether flecainide inhibits waves in the intact cardiomyocyte and to elucidate the mechanism...
  17. ncbi request reprint Myocardial calcium signalling and arrhythmia pathogenesis
    Mark Scoote
    Department of Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, Dovehouse Street, London SW3 6LY, UK
    Biochem Biophys Res Commun 322:1286-309. 2004
    ..Potential opportunities for new anti arrhythmic therapeutic strategies based on these recent insights are also discussed...
  18. ncbi request reprint Pharmacological regulators of intracellular calcium release channels
    Duncan J West
    Cardiac Medicine, National Heart and Lung Institute, Imperial College London, Guy Scadding Building, London, UK
    Curr Pharm Des 13:2428-42. 2007
    ..In this article, we review the wide array of pharmacological agents that interact directly with intracellular Ca(2+) release channels and describe the mechanisms underlying their ability to modify channel function...
  19. ncbi request reprint The cardiac ryanodine receptor (calcium release channel): emerging role in heart failure and arrhythmia pathogenesis
    Mark Scoote
    Department of Cardiac Medicine, National Heart and Lung Institute, Imperial College of Science, Technology and Medicine, Dovehouse Street, London, UK
    Cardiovasc Res 56:359-72. 2002
    ....
  20. pmc Block of the ryanodine receptor channel by neomycin is relieved at high holding potentials
    Fiona Mead
    Department of Cardiac Medicine, National Heart and Lung Institute, Imperial College of Science, Technology and Medicine, London SW3 6LY, United Kingdom
    Biophys J 82:1953-63. 2002
    ..These observations indicate that, under appropriate conditions, neomycin is capable of passing through the RyR channel...
  21. ncbi request reprint Ion conduction and selectivity in the ryanodine receptor channel
    Alan J Williams
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Dovehouse St, London SW3 6LY, United Kingdom
    Front Biosci 7:d1223-30. 2002
    ....
  22. ncbi request reprint Probing luminal negative charge in the type 3 ryanodine receptor
    Nicholas Pugh
    The Laboratory of Functional Immunogenetics, The Babraham Institute, Cambridge CB2 4AT, UK
    Biochem Biophys Res Commun 337:1072-9. 2005
    ..The change in affinity may be due to altered negative charge density at the site of interaction...
  23. pmc A model of the putative pore region of the cardiac ryanodine receptor channel
    William Welch
    Department of Biochemistry, University of Nevada School of Medicine, Reno, Nevada 89557, USA
    Biophys J 87:2335-51. 2004
    ..These simulations suggest that the model RyR2 pore is permeable to Ca2+ and K+ with rates of translocation greater for K+. In contrast, simulations indicate that tetraethylammonium blocks movement of metal cations...
  24. ncbi request reprint Ryanodine receptor interaction with the SNARE-associated protein snapin
    Spyros Zissimopoulos
    Wales Heart Research Institute, Department of Cardiology, Cardiff University School of Medicine, Heath Park, UK
    J Cell Sci 119:2386-97. 2006
    ..Thus, regulation of the association between ryanodine receptor and snapin might constitute part of the elusive molecular mechanism by which ryanodine-sensitive Ca(2+) stores modulate neurosecretion...
  25. pmc Ryanodine receptor regulation by intramolecular interaction between cytoplasmic and transmembrane domains
    Christopher H George
    Wales Heart Research Institute, Department of Cardiology, University of Wales College of Medicine, Cardiff, United Kingdom CF14 4XN
    Mol Biol Cell 15:2627-38. 2004
    ..These results provide compelling evidence that specific interaction between cytoplasmic and transmembrane domains is an important mechanism in the intrinsic modulation of RyR Ca(2+) release channels...
  26. ncbi request reprint Residue Gln4863 within a predicted transmembrane sequence of the Ca2+ release channel (ryanodine receptor) is critical for ryanodine interaction
    Ruiwu Wang
    Cardiovascular Research Group, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 278:51557-65. 2003
    ..Together these results suggest that the TM10 sequence and in particular the Q4863 residue constitute an important determinant of ryanodine interaction...