Siouxsie Wiles

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. pmc In vivo bioluminescence imaging of the murine pathogen Citrobacter rodentium
    Siouxsie Wiles
    Division of Cell and Molecular Biology, Faculty of Natural Sciences, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 74:5391-6. 2006
  2. ncbi request reprint Modelling infectious disease - time to think outside the box?
    Siouxsie Wiles
    Division of Cell and Molecular Biology, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
    Nat Rev Microbiol 4:307-12. 2006
  3. pmc Role of NleH, a type III secreted effector from attaching and effacing pathogens, in colonization of the bovine, ovine, and murine gut
    Cordula Hemrajani
    Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 76:4804-13. 2008
  4. ncbi request reprint Cell attachment properties and infectivity of host-adapted and environmentally adapted Citrobacter rodentium
    Anne L Bishop
    Division of Cell and Molecular Biology, Flowers Building, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Microbes Infect 9:1316-24. 2007
  5. ncbi request reprint Emergence of a 'hyperinfectious' bacterial state after passage of Citrobacter rodentium through the host gastrointestinal tract
    Siouxsie Wiles
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    Cell Microbiol 7:1163-72. 2005
  6. ncbi request reprint Citrobacter rodentium of mice and man
    Rosanna Mundy
    Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London SW7 2AZ, UK
    Cell Microbiol 7:1697-706. 2005
  7. pmc Rapid in vivo assessment of drug efficacy against Mycobacterium tuberculosis using an improved firefly luciferase
    Nuria Andreu
    MRC Centre for Molecular Bacteriology and Infection, Department of Medicine, Imperial College London, London, UK
    J Antimicrob Chemother 68:2118-27. 2013
  8. pmc Rapid measurement of antituberculosis drug activity in vitro and in macrophages using bioluminescence
    Nuria Andreu
    Microbiology, Department of Medicine, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK
    J Antimicrob Chemother 67:404-14. 2012
  9. pmc Alternative luciferase for monitoring bacterial cells under adverse conditions
    Siouxsie Wiles
    Centre for Molecular Microbiology and Infection, Faculty of Medicine, Imperial College London, London SW7 2AZ, United Kingdom
    Appl Environ Microbiol 71:3427-32. 2005
  10. pmc Noninvasive biophotonic imaging for studies of infectious disease
    Nuria Andreu
    Department of Medicine, Imperial College London, London, UK
    FEMS Microbiol Rev 35:360-94. 2011

Collaborators

Detail Information

Publications24

  1. pmc In vivo bioluminescence imaging of the murine pathogen Citrobacter rodentium
    Siouxsie Wiles
    Division of Cell and Molecular Biology, Faculty of Natural Sciences, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 74:5391-6. 2006
    ..In addition, BLI revealed that C. rodentium colonizes the rectum, a site previously unreported for this pathogen...
  2. ncbi request reprint Modelling infectious disease - time to think outside the box?
    Siouxsie Wiles
    Division of Cell and Molecular Biology, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
    Nat Rev Microbiol 4:307-12. 2006
    ..Focusing on mouse models of bacterial pathogens, we discuss some of these discrepancies and suggest ways of improving model systems in the future...
  3. pmc Role of NleH, a type III secreted effector from attaching and effacing pathogens, in colonization of the bovine, ovine, and murine gut
    Cordula Hemrajani
    Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 76:4804-13. 2008
    ..Consistent with this, we found that the nleH mutant triggered a significantly lower tumor necrosis factor alpha response than the wild-type strain...
  4. ncbi request reprint Cell attachment properties and infectivity of host-adapted and environmentally adapted Citrobacter rodentium
    Anne L Bishop
    Division of Cell and Molecular Biology, Flowers Building, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Microbes Infect 9:1316-24. 2007
    ..rodentium cultured in laboratory media. Thus, the 'hyper-infectious' host-adapted state, allowing efficient transmission and colonisation of naive hosts, is transient in nature and gradually lost after shedding into the environment...
  5. ncbi request reprint Emergence of a 'hyperinfectious' bacterial state after passage of Citrobacter rodentium through the host gastrointestinal tract
    Siouxsie Wiles
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    Cell Microbiol 7:1163-72. 2005
    ..In addition, the 'hyperinfectious' phenotype was found to be transient with one overnight passage in rich medium sufficient to return C. rodentium to 'culture' infectivity...
  6. ncbi request reprint Citrobacter rodentium of mice and man
    Rosanna Mundy
    Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London SW7 2AZ, UK
    Cell Microbiol 7:1697-706. 2005
    ..Here we review the literature of C. rodentium from its emergence in the mid-1960s to the most contemporary reports of colonization, pathogenesis, transmission and immunity...
  7. pmc Rapid in vivo assessment of drug efficacy against Mycobacterium tuberculosis using an improved firefly luciferase
    Nuria Andreu
    MRC Centre for Molecular Bacteriology and Infection, Department of Medicine, Imperial College London, London, UK
    J Antimicrob Chemother 68:2118-27. 2013
    ..The objective of this work was to develop a bioluminescence-based mouse model of tuberculosis to assess antibiotic efficacy against M. tuberculosis in vivo...
  8. pmc Rapid measurement of antituberculosis drug activity in vitro and in macrophages using bioluminescence
    Nuria Andreu
    Microbiology, Department of Medicine, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK
    J Antimicrob Chemother 67:404-14. 2012
    ..The aim of this work was to validate and standardize the use of luminescent M. tuberculosis strains to test the activity of antibacterial drugs in vitro and inside macrophages in a 96-well format...
  9. pmc Alternative luciferase for monitoring bacterial cells under adverse conditions
    Siouxsie Wiles
    Centre for Molecular Microbiology and Infection, Faculty of Medicine, Imperial College London, London SW7 2AZ, United Kingdom
    Appl Environ Microbiol 71:3427-32. 2005
    ..The work presented here demonstrated the utility of the copepod luciferase bioluminescent reporter as an alternative to bacterial luciferase, particularly for monitoring responses to environmental stress stimuli...
  10. pmc Noninvasive biophotonic imaging for studies of infectious disease
    Nuria Andreu
    Department of Medicine, Imperial College London, London, UK
    FEMS Microbiol Rev 35:360-94. 2011
    ..Here, we review the technology of BPI, from the instrumentation through to the generation of a photonic signal, and illustrate how the technique is shedding light on infection dynamics in vivo...
  11. ncbi request reprint Use of biophotonic imaging as a training aid for administration of substances in laboratory rodents
    Siouxsie Wiles
    Division of Cell and Molecular Biology, Natural Sciences, Imperial College London, London SW7 2AZ, UK
    Lab Anim 41:321-8. 2007
    ..The data presented suggest that this technology is ideal for use as a teaching aid and may also prove valuable in assessing the effectiveness of 'complex' and novel administration routes in 'realtime'...
  12. pmc Non-invasive monitoring of Streptococcus pyogenes vaccine efficacy using biophotonic imaging
    Faraz M Alam
    Infectious Diseases and Immunity, Department of Medicine, Imperial College London, London, United Kingdom
    PLoS ONE 8:e82123. 2013
    ..pyogenes bioluminescence emission over the course of nasopharyngeal infection. The work suggests the potential for BPI to be used in the non-invasive longitudinal evaluation of potential S. pyogenes vaccines. ..
  13. pmc Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens
    Olivier Marches
    CMMI, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 73:8411-7. 2005
    ..We report that NleC and NleD are translocated into host cells via this system. Deletion mutants induced attaching and effacing lesions in vitro, while infection of calves or lambs showed that neither gene was required for colonization...
  14. pmc Optimisation of bioluminescent reporters for use with mycobacteria
    Nuria Andreu
    Department of Medicine, Imperial College London, London, UK
    PLoS ONE 5:e10777. 2010
    ..Despite the extensive use of luminescent reporters in mycobacteria, the resultant luminescent strains have not been fully applied to BLI...
  15. pmc EspJ is a prophage-carried type III effector protein of attaching and effacing pathogens that modulates infection dynamics
    Sivan Dahan
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 73:679-86. 2005
    ....
  16. doi request reprint Bioluminescent monitoring of in vivo colonization and clearance dynamics by light-emitting bacteria
    Siouxsie Wiles
    Department of Infectious Diseases and Immunity, Imperial College London, London, UK
    Methods Mol Biol 574:137-53. 2009
    ..Here we describe the protocols required to monitor colonization and clearance dynamics using bioluminescent bacteria that are lux-, luc-, or Gluc-positive...
  17. ncbi request reprint In vivo imaging of gene transfer to the respiratory tract
    Uta Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW3 6LR, UK
    Biomaterials 29:1533-40. 2008
    ..45, p<0.05). To our knowledge these studies show for the first time that this non-invasive method of assessing pulmonary gene transfer is viable for evaluating non-viral gene transfer agents...
  18. pmc Inactivation of the CovR/S virulence regulator impairs infection in an improved murine model of Streptococcus pyogenes naso-pharyngeal infection
    Faraz M Alam
    Section of Infectious Diseases and Immunity, Department of Medicine, Imperial College London, London, United Kingdom
    PLoS ONE 8:e61655. 2013
    ..This may explain why S. pyogenes with altered covR/S have not become prevalent in community infections despite possessing a selective advantage in invasive infection...
  19. ncbi request reprint Organ specificity, colonization and clearance dynamics in vivo following oral challenges with the murine pathogen Citrobacter rodentium
    Siouxsie Wiles
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    Cell Microbiol 6:963-72. 2004
    ..A bioluminescent eae (encoding the outer membrane adhesin intimin) C. rodentium mutant failed to establish long-term colonization, although low levels of bacteria could be recovered for up to 3 days post challenge from the caecum...
  20. pmc Tetracycline-inducible gene regulation in mycobacteria
    Marian C J Blokpoel
    Department of Infectious Diseases and Microbiology, Centre for Molecular Microbiology and Infection, Imperial College London South Kensington campus, London SW7 2AZ, UK
    Nucleic Acids Res 33:e22. 2005
    ..These results demonstrate the potential of this tetracycline-regulated system for the manipulation of mycobacterial gene expression inside and outside cells...
  21. ncbi request reprint Calibration and deployment of custom-designed bioreporters for protecting biological remediation consortia from toxic shock
    Siouxsie Wiles
    Molecular Microbial Ecology Section, CEH Oxford, Mansfield Road, Oxford, OX1 3SR, UK
    Environ Microbiol 7:260-9. 2005
    ....
  22. pmc Essential role of the type III secretion system effector NleB in colonization of mice by Citrobacter rodentium
    Michelle Kelly
    Department of Microbiology, Monash University, Victoria 3800, Australia
    Infect Immun 74:2328-37. 2006
    ..rodentium, nleC, nleD, or nleE, had no effect on host colonization or disease. These results suggest that nleB encodes an important virulence determinant of A/E pathogens...
  23. pmc The p50 subunit of NF-kappaB is critical for in vivo clearance of the noninvasive enteric pathogen Citrobacter rodentium
    Alison Dennis
    Centre for Infectious Disease, Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, 4 Newark Street London E1 2AT, United Kingdom
    Infect Immun 76:4978-88. 2008
    ..These data also demonstrate that non-NF-kappaB-dependent defenses are insufficient to control C. rodentium infection, and hence, the NF-kappaB p50 subunit is critical for defense against this noninvasive pathogen...
  24. ncbi request reprint Development of bespoke bioluminescent reporters with the potential for in situ deployment within a phenolic-remediating wastewater treatment system
    Siouxsie Wiles
    Molecular Microbial Ecology Laboratory, Centre for Ecology and Hydrology Oxford, Mansfield Road, Oxford OX1 3SR, UK
    J Microbiol Methods 55:667-77. 2003
    ....