Yoshinobu Takakura

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. doi request reprint Structural and immunostimulatory properties of Y-shaped DNA consisting of phosphodiester and phosphorothioate oligodeoxynucleotides
    Nao Matsuoka
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 148:311-6. 2010
  2. ncbi request reprint Macrophage-dependent clearance of systemically administered B16BL6-derived exosomes from the blood circulation in mice.
    Takafumi Imai
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Extracell Vesicles 4:26238. 2015
  3. ncbi request reprint Hsp-based tumor vaccines: state-of-the-art and future directions
    Yoshinobu Takakura
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Curr Opin Mol Ther 9:385-91. 2007
  4. doi request reprint Development of a novel Hsp70-based DNA vaccine as a multifunctional antigen delivery system
    Ayumi Yamaoka
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 142:411-5. 2010
  5. doi request reprint Simultaneous delivery of doxorubicin and immunostimulatory CpG motif to tumors using a plasmid DNA/doxorubicin complex in mice
    Yumiko Mizuno
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 141:252-9. 2010
  6. doi request reprint Effect of the content of unmethylated CpG dinucleotides in plasmid DNA on the sustainability of transgene expression
    Masaru Mitsui
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Gene Med 11:435-43. 2009
  7. ncbi request reprint Improved anti-cancer effect of interferon gene transfer by sustained expression using CpG-reduced plasmid DNA
    Hiroki Kawano
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    Int J Cancer 121:401-6. 2007
  8. ncbi request reprint Design of PCR-amplified DNA fragments for in vivo gene delivery: size-dependency on stability and transgene expression
    Kazuhiro Hirata
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pharm Sci 96:2251-61. 2007
  9. ncbi request reprint Effects of methyl-beta-cyclodextrin treatment on secretion profile of interferon-beta and zonula occuludin-1 architecture in Madin-Darby canine kidney cell monolayers
    Masato Maruyama
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Biol Pharm Bull 32:910-5. 2009
  10. doi request reprint Persistent interferon transgene expression by RNA interference-mediated silencing of interferon receptors
    Yuki Takahashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Gene Med 12:739-46. 2010

Collaborators

Detail Information

Publications107 found, 100 shown here

  1. doi request reprint Structural and immunostimulatory properties of Y-shaped DNA consisting of phosphodiester and phosphorothioate oligodeoxynucleotides
    Nao Matsuoka
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 148:311-6. 2010
    ..In conclusion, Y-shape formation and PS substitution can be used simultaneously to increase the immunostimulatory activity of CpG ODN, but extensive substitution should be avoided because it diminishes the benefits of Y-shape formation...
  2. ncbi request reprint Macrophage-dependent clearance of systemically administered B16BL6-derived exosomes from the blood circulation in mice.
    Takafumi Imai
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Extracell Vesicles 4:26238. 2015
    ..These results indicate that macrophages play important roles in the clearance of intravenously injected B16BL6 exosomes from the systemic circulation...
  3. ncbi request reprint Hsp-based tumor vaccines: state-of-the-art and future directions
    Yoshinobu Takakura
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Curr Opin Mol Ther 9:385-91. 2007
    ..Successful cancer immunotherapy with Hsp-based tumor vaccines will depend on the results obtained from both clinical trials and preclinical studies...
  4. doi request reprint Development of a novel Hsp70-based DNA vaccine as a multifunctional antigen delivery system
    Ayumi Yamaoka
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 142:411-5. 2010
    ..These results indicate that the Hsp70-based DNA vaccine is useful as a multifunctional antigen delivery system to induce the antigen-specific immune response...
  5. doi request reprint Simultaneous delivery of doxorubicin and immunostimulatory CpG motif to tumors using a plasmid DNA/doxorubicin complex in mice
    Yumiko Mizuno
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 141:252-9. 2010
    ..These results indicate that CpG plasmid DNA is an effective polymer that inhibits tumor growth by delivering both a proinflammatory signal and anticancer agent to tumor tissues...
  6. doi request reprint Effect of the content of unmethylated CpG dinucleotides in plasmid DNA on the sustainability of transgene expression
    Masaru Mitsui
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Gene Med 11:435-43. 2009
    ..A further extension of the duration of transgene expression could be achieved by controlling the number and location of CpG motifs in plasmid DNA...
  7. ncbi request reprint Improved anti-cancer effect of interferon gene transfer by sustained expression using CpG-reduced plasmid DNA
    Hiroki Kawano
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    Int J Cancer 121:401-6. 2007
    ..These results indicate that long-term expression of IFN can be achieved by CpG-reduced pDNA and sustained IFN gene expression results in enhanced therapeutic effects of IFN gene transfer against tumor metastasis...
  8. ncbi request reprint Design of PCR-amplified DNA fragments for in vivo gene delivery: size-dependency on stability and transgene expression
    Kazuhiro Hirata
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pharm Sci 96:2251-61. 2007
    ..Their stabilization against nucleases will further increase the potential of such short, structure-controlled and synthetic DNA fragments for in vivo gene delivery...
  9. ncbi request reprint Effects of methyl-beta-cyclodextrin treatment on secretion profile of interferon-beta and zonula occuludin-1 architecture in Madin-Darby canine kidney cell monolayers
    Masato Maruyama
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Biol Pharm Bull 32:910-5. 2009
    ..These results suggest that lipid rafts may not be crucially involved in the regulation of secretion polarity of IFN-beta in the epithelial cells...
  10. doi request reprint Persistent interferon transgene expression by RNA interference-mediated silencing of interferon receptors
    Yuki Takahashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Gene Med 12:739-46. 2010
    ..Silencing the expression of IFNAR and IFNGR, the receptors for type I and II IFNs, respectively, in cells expressing IFNs may prolong transgene expression of IFNs...
  11. doi request reprint Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
    Naomi Takiguchi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, 606 8501, Japan
    Pharm Res 28:702-11. 2011
    ..Hydrodynamic injection has been shown to reactivate silenced transgene expression in mouse liver. In this study, the roles of inflammatory cytokines and reactive oxygen species (ROS) in the reactivation were examined...
  12. ncbi request reprint Macrophage activation by a DNA/cationic liposome complex requires endosomal acidification and TLR9-dependent and -independent pathways
    Kei Yasuda
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29, Yoshidashimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    J Leukoc Biol 77:71-9. 2005
    ..However, the cytokine production was not completely abolished, suggesting that the DNA/cationic liposome complex can induce macrophage activation via TLR9-dependent and -independent pathways...
  13. doi request reprint Enhanced generation of cytotoxic T lymphocytes by increased cytosolic delivery of MHC class I epitope fused to mouse heat shock protein 70 via polyhistidine conjugation
    Seiji Takemoto
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Science, Kyoto University, Sakyo ku, Kyoto, Sakyo ku, Japan
    J Control Release 135:11-8. 2009
    ..These findings suggest that polyhistidine fusion is a useful strategy to increase the potential of Hsp-based vaccination...
  14. ncbi request reprint Enhanced antigen-specific antibody production following polyplex-based DNA vaccination via the intradermal route in mice
    Atsushi Kawase
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Vaccine 24:5535-45. 2006
    ..Thus, the present study has demonstrated that the immune responses could be biased towards a Th2-type response by polyplex-based DNA vaccination through manipulation of not only pDNA disposition but also dendritic cell migration...
  15. doi request reprint Enhanced CTL response by controlled intracellular trafficking of antigen in dendritic cells following DNA vaccination
    Keiko Isaji
    Department of Biopharmaceutics and Drug Metabolism Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 135:227-33. 2009
    ....
  16. doi request reprint Sustained exogenous expression of therapeutic levels of IFN-gamma ameliorates atopic dermatitis in NC/Nga mice via Th1 polarization
    Kayoko Hattori
    Department of Biopharmaceutics and Drug Metabolism, Doshisha Women s College of Liberal Arts, Kyotanabe, Kyoto, Japan
    J Immunol 184:2729-35. 2010
    ..These results indicate that an extraordinary sustained IFN-gamma expression induces favorable immunological changes, leading to a Th1-dominant state in the atopic dermatitis model...
  17. doi request reprint Biodegradable CpG DNA hydrogels for sustained delivery of doxorubicin and immunostimulatory signals in tumor-bearing mice
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    Biomaterials 32:488-94. 2011
    ..These results show that CpG DNA hydrogels are an effective sustained system for delivery of immunostimulatory signals to TLR9-positive immune cells and DXR to cancer cells...
  18. doi request reprint Prolonged circulation half-life of interferon γ activity by gene delivery of interferon γ-serum albumin fusion protein in mice
    Noriko Miyakawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Pharm Sci 100:2350-7. 2011
    ..These results indicate that gene delivery of IFNγ-MSA is a promising approach to prolong the circulation half-life of IFNγ activity...
  19. doi request reprint Gene delivery of albumin binding peptide-interferon-gamma fusion protein with improved pharmacokinetic properties and sustained biological activity
    Noriko Miyakawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Science, Kyoto University, Kyoto 606 8501, Japan
    J Pharm Sci 102:3110-8. 2013
    ..These results indicate that the fusion of ABP is a useful approach to achieving prolonged retention in the blood circulation through binding to serum albumin and retaining biological activity...
  20. doi request reprint Enhanced generation of cytotoxic T lymphocytes by heat shock protein 70 fusion proteins harboring both CD8(+) T cell and CD4(+) T cell epitopes
    Seiji Takemoto
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Mol Pharm 7:1715-23. 2010
    ..These results demonstrated that Hsp70 fusion protein harboring both pepI and pepII is a useful option for Hsp70-based antigen delivery systems...
  21. ncbi request reprint Hepatic delivery of particulates in the submicron range by a hydrodynamics-based procedure: implications for particulate gene delivery systems
    Naoki Kobayashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Gene Med 6:455-63. 2004
    ..v.) injection of DNA, i.e. a hydrodynamics-based transfection procedure, is known to be an efficient and liver-specific method of in vivo gene delivery. However, little is available on an applicable particle size in the procedure...
  22. doi request reprint Comparison of the type of liposome involving cytokine production induced by non-CpG Lipoplex in macrophages
    Sachiyo Yasuda
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29, Yoshidashimoadachi cho, Sakyo ku, Kyoto, 606 8501, Japan
    Mol Pharm 7:533-42. 2010
    ..The present study demonstrates that cationic liposomes should be selected based on these findings for optimization of DNA-based therapies using lipoplexes...
  23. doi request reprint Injection site-dependent induction of immune response by DNA vaccine: comparison of skin and spleen as a target for vaccination
    Xin Guan
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Gene Med 12:301-9. 2010
    ..In addition, the spleen is the largest organ in the lymphatic system and contains a variety of types of immune cells, including lymphocytes, macrophages and dendritic cells. Thus, it can be a promising target for DNA vaccination...
  24. doi request reprint 5'-Phosphate oligodeoxynucleotides enhance the phosphodiester-CpG DNA-induced inflammatory response in macrophages
    Hiroyuki Yoshida
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Eur J Immunol 41:425-36. 2011
    ..These results imply that PO-CpG DNA-dependent inflammatory responses are increased by DNA molecules with a 5'-phosphate; such molecules could therefore be considered as exacerbating factors for CpG motif-related inflammation...
  25. ncbi request reprint Therapeutic effect of intravenous delivery of lipoplexes containing the interferon-beta gene and poly I: poly C in a murine lung metastasis model
    Fuminori Sakurai
    Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Cancer Gene Ther 10:661-8. 2003
    ..Thus, the local IFN-beta in the lung delivered by intravenous administration of lipoplex containing IFN-beta gene or pI:pC may be a convenient and useful method of inhibiting established metastatic lung tumors...
  26. ncbi request reprint Secretion mode and subcellular localization of human interferon-beta exogenously expressed in porcine renal epithelial LLC-PK1 cells
    Teruko Nishio
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Japan
    Biol Pharm Bull 27:1653-5. 2004
    ..These results suggest that the secretion polarity of HuIFN-beta is regulated by a post trans-Golgi network in a cell type-dependent manner...
  27. ncbi request reprint Gene silencing in primary and metastatic tumors by small interfering RNA delivery in mice: quantitative analysis using melanoma cells expressing firefly and sea pansy luciferases
    Yuki Takahashi
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Control Release 105:332-43. 2005
    ..These results indicate that the both RNAi effectors have a potential to silence target gene in tumor cells in vivo when successfully delivered to tumor cells...
  28. ncbi request reprint Cellular uptake and activation characteristics of naked plasmid DNA and its cationic liposome complex in human macrophages
    Ikuko Yamane
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshidashimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Int J Pharm 305:145-53. 2005
    ..These findings form an important basis for DNA-based therapies including gene therapy and DNA vaccination...
  29. doi request reprint Cellular activation by plasmid DNA in various macrophages in primary culture
    Hiroyuki Yoshida
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pharm Sci 97:4575-85. 2008
    ..These results indicate that splenic macrophages and hepatic NPCs are closely involved in TNF-alpha production in response to pDNA...
  30. doi request reprint Quantitative and temporal analysis of gene silencing in tumor cells induced by small interfering RNA or short hairpin RNA expressed from plasmid vectors
    Yuki Takahashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Pharm Sci 98:74-80. 2009
    ..These statistical considerations demonstrated that U6 promoter-driven shRNA expressing pDNA is the most effective in inducing gene silencing effect as far as the intensity and duration of RNAi effect is concerned...
  31. doi request reprint TLR9-dependent systemic interferon-beta production by intravenous injection of plasmid DNA/cationic liposome complex in mice
    Hiroyuki Yoshida
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Gene Med 11:708-17. 2009
    ..Studies of the pro-inflammatory cytokine response to lipoplex have shown the importance of the unmethylated CpG dinucleotide (CpG motif) and its receptor, Toll-like receptor (TLR)-9...
  32. doi request reprint Inhibition of surgical trauma-enhanced peritoneal dissemination of tumor cells by human catalase derivatives in mice
    Chika Nishizaki
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Free Radic Biol Med 51:773-9. 2011
    ..These results indicate that hCAT-R9 and hCAT-ABP, both of which have a long retention time in the peritoneal cavity, can be effective at inhibiting surgery-induced peritoneal metastasis...
  33. doi request reprint Accelerated growth of B16BL6 tumor in mice through efficient uptake of their own exosomes by B16BL6 cells
    Akihiro Matsumoto
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    Cancer Sci 108:1803-1810. 2017
    ..These results indicate that B16BL6 cells secrete and take up their own exosomes to induce their proliferation and inhibit their apoptosis, which promotes tumor progression...
  34. doi request reprint Self-assembling DNA hydrogel-based delivery of immunoinhibitory nucleic acids to immune cells
    Yu Nishida
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    Nanomedicine 12:123-30. 2016
    ..In this article, the authors described hydrogel-based platform for the delivery of the inhibitory oligodeoxynucleotides for enhanced efficacy. The positive findings could indicate a way for the future...
  35. doi request reprint Fibronectin inhibits cytokine production induced by CpG DNA in macrophages without direct binding to DNA
    Hiroyuki Yoshida
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimo Adachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Cytokine 60:162-70. 2012
    ....
  36. doi request reprint Design and development of nanosized DNA assemblies in polypod-like structures as efficient vehicles for immunostimulatory CpG motifs to immune cells
    Kohta Mohri
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    ACS Nano 6:5931-40. 2012
    ..7 cells but reduced the stability in serum. These results indicate that CpG DNA-containing polypodna preparations with six or more pods are a promising nanosized device with biodegradability and high immunostimulatory activity...
  37. ncbi request reprint Hydrodynamics-based procedure involves transient hyperpermeability in the hepatic cellular membrane: implication of a nonspecific process in efficient intracellular gene delivery
    Naoki Kobayashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Gene Med 6:584-92. 2004
    ..In this report, we have investigated possible mechanisms for the intracellular transport of naked pDNA by this procedure...
  38. ncbi request reprint Significant role of liver sinusoidal endothelial cells in hepatic uptake and degradation of naked plasmid DNA after intravenous injection
    Jin Hisazumi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Pharm Res 21:1223-8. 2004
    ..Uptake and degradation of naked plasmid DNA (pDNA) by liver sinusoidal endothelial cells (LSECs) were investigated...
  39. ncbi request reprint Simultaneous detection of DsRed2-tagged and EGFP-tagged human beta-interferons in the same single cells
    Masato Maruyama
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biochem 93:497-502. 2004
    ..This approach will be useful for investigating subcellular localization of not only cell resident proteins but also secretory proteins...
  40. ncbi request reprint Sequence-specific suppression of mdr1a/1b expression in mice via RNA interference
    Yumi Matsui
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    Pharm Res 22:2091-8. 2005
    ....
  41. ncbi request reprint Pharmacokinetics of plasmid DNA-based non-viral gene medicine
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Adv Genet 53:47-68. 2005
    ....
  42. ncbi request reprint Suppression of tumor growth by intratumoral injection of short hairpin RNA-expressing plasmid DNA targeting beta-catenin or hypoxia-inducible factor 1alpha
    Yuki Takahashi
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Control Release 116:90-5. 2006
    ..The growth of the intradermal tumor was significantly (P<0.05) suppressed by the treatment. In conclusion, tumor growth was successfully inhibited by the intratumoral delivery of pshbeta-catenin or pshHIF1alpha...
  43. pmc Induction of tumor-specific immune response by gene transfer of Hsp70-cell-penetrating peptide fusion protein to tumors in mice
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Japan
    Mol Ther 18:421-8. 2010
    ..These results provide a new therapeutic method to induce tumor-specific antitumor immunity without identifying nor isolating tumor-associated antigens...
  44. doi request reprint Bootstrap method-based estimation of the minimum sample number for obtaining pharmacokinetic parameters in preclinical experiments
    Seiji Takemoto
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Science, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pharm Sci 99:2176-84. 2010
    ..An issue that one-point sampling data cannot separately assess inter- and intra-individual variability is discussed...
  45. ncbi request reprint [Control of spatiotemporal distribution of interferon γ by genetically fusing functional peptides]
    Mitsuru Ando
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Yakugaku Zasshi 132:1399-406. 2012
    ..These results provide experimental evidence that IFNγ gene therapy can be a useful treatment for a variety of diseases...
  46. doi request reprint Self-assembling CpG DNA nanoparticles for efficient antigen delivery and immunostimulation
    Sakulrat Rattanakiat
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Eur J Pharm Sci 47:352-8. 2012
    ..These results indicate that self-assembling nanoparticles of Chol-CpG ODN are effective for inducing antigen-specific immune responses because of the high immunostimulatory activity, ability to incorporate antigens and tropism to APCs...
  47. doi request reprint Increased immunostimulatory activity of polypod-like structured DNA by ligation of the terminal loop structures
    Kohta Mohri
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 163:285-92. 2012
    ..These results indicate that circular tetrapodna with no terminal ends is more effective than its non-ligated counterparts in the presence of serum...
  48. ncbi request reprint Exogenous expression of interferon-beta in cultured brain microvessel endothelial cells
    Masaru Nakamura
    Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biol Pharm Bull 27:1441-3. 2004
    ..These findings provide important basic information about therapeutic secretory protein gene delivery to BMECs...
  49. ncbi request reprint Cholesterol is required for the polarized secretion of erythropoietin in Madin-Darby canine kidney cells
    Masato Maruyama
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Japan
    Arch Biochem Biophys 438:174-81. 2005
    ..These results indicate that apical secretion of EPO involves a cholesterol-dependent mechanism probably not involving lipid rafts...
  50. ncbi request reprint Induction of antigen-specific cytotoxic T lymphocytes by immunization with negatively charged soluble antigen through scavenger receptor-mediated delivery
    Yasuomi Yamasaki
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Vaccine 25:85-91. 2007
    ..G7 tumor cells. In conclusion, the present study demonstrates that the introduction of negative charges to soluble antigens will be a useful strategy for the development of vaccines...
  51. pmc DNA and its cationic lipid complexes induce CpG motif-dependent activation of murine dendritic cells
    Takaharu Yoshinaga
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Immunology 120:295-302. 2007
    ..These results also suggest that DC are the major cells that respond to naked CpG DNA in vivo, although both DC and macrophages will release inflammatory cytokines after the administration of a DNA/cationic lipid complex...
  52. ncbi request reprint Effects of inflammatory response on in vivo transgene expression by plasmid DNA in mice
    Keiko Kako
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pharm Sci 97:3074-83. 2008
    ....
  53. ncbi request reprint Unique cytokine production profile following stimulation with DNA in macrophages from NZB/W F1 mice
    Yoshiyuki Ogawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Biol Pharm Bull 31:1244-9. 2008
    ..These results suggest that M Phis of NZB/W mice have altered responsiveness to DNA and this might elevate the antigenicity of nucleosomes to induce the production of anti-nucleosome antibodies...
  54. doi request reprint Constant and steady transgene expression of interferon-γ by optimization of plasmid construct for safe and effective interferon-γ gene therapy
    Mitsuru Ando
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Science, Kyoto University, Kyoto, Japan
    J Gene Med 14:288-95. 2012
    ....
  55. doi request reprint Controlling the kinetics of interferon transgene expression for improved gene therapy
    Kanitta Watcharanurak
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Drug Target 20:764-9. 2012
    ..In this review, we aim to present experimental evidence about the relationships among the properties of plasmid vectors expressing IFNs, the kinetics of transgene expression, and therapeutic effects as well as safety issues...
  56. ncbi request reprint Vector-based in vivo RNA interference: dose- and time-dependent suppression of transgene expression
    Naoki Kobayashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
    J Pharmacol Exp Ther 308:688-93. 2004
    ..Taken together, these results demonstrate that vector-based in vivo RNAi is a dose- and time-dependent process and offers the possibility of suppressing endogenous targets in a variety of somatic cells...
  57. ncbi request reprint Moment analysis for kinetics of gene silencing by RNA interference
    Yuki Takahashi
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Biotechnol Bioeng 93:816-9. 2006
    ..The proposed kinetic analysis helps to understand the RNAi effect in a quantitative and time-dependent manner, which will be beneficial for designing RNAi-based gene silencing for both experimental and therapeutic purposes...
  58. ncbi request reprint Inhibition of metastatic tumor growth by targeted delivery of antioxidant enzymes
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Japan
    J Control Release 109:101-7. 2005
    ..These results indicate that targeted or sustained delivery of catalase to sites where tumor cells metastasize is a promising approach for inhibiting metastatic tumor growth...
  59. doi request reprint Reactivation of silenced transgene expression in mouse liver by rapid, large-volume injection of isotonic solution
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Hum Gene Ther 19:1009-20. 2008
    ....
  60. doi request reprint Enhancement of antiproliferative activity of interferons by RNA interference-mediated silencing of SOCS gene expression in tumor cells
    Yuki Takahashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Cancer Sci 99:1650-5. 2008
    ..These results indicate that silencing SOCS gene expression can be an effective strategy to enhance the antitumor effect of IFN under conditions in which the SOCS gene expression is upregulated by IFN...
  61. ncbi request reprint [Optimization of gene therapy effect by spaciotemporal control of expressed proteins]
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Yakugaku Zasshi 130:1505-11. 2010
    ..Thus, designing plasmid vectors is a promising approach not only to controlling the spatiotemporal distribution of proteins, but also to increasing the therapeutic potency of in vivo gene therapy...
  62. doi request reprint Self-assembling DNA dendrimer for effective delivery of immunostimulatory CpG DNA to immune cells
    Kohta Mohri
    Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Biomacromolecules 16:1095-101. 2015
    ..These results indicate that the ligation-free, self-assembling DNA dendrimers are a potent system for the delivery of immunostimulatory CpG DNA to immune cells. ..
  63. doi request reprint Transplantation of insulin-secreting multicellular spheroids for the treatment of type 1 diabetes in mice
    Kosuke Kusamori
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshidashimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 173:119-24. 2014
    ..These results indicate that insulin-secreting NIT-1 spheroids prepared using PNIPAAm-coated PDMS-based microwells are more effective for the treatment of type 1 diabetes than dissociated cells in suspension. ..
  64. ncbi request reprint Subcellular trafficking of exogenously expressed interferon-beta in Madin-Darby canine kidney cells
    Masato Maruyama
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Cell Physiol 201:117-25. 2004
    ..These results suggest that stably and transiently expressed IFN-betas, albeit co-localized at TGN, were transported through apparently discriminated post-TGN routes...
  65. ncbi request reprint Manipulation of local disposition and gene expression characteristics of plasmid DNA following intramuscular administration by complexation with cationic macromolecule
    Atsushi Kawase
    Department of Biopharmaceutics and Drug Metabolism Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Int J Pharm 293:291-301. 2005
    ..These results suggest that the immune responses might be manipulated by complexation presumably due to the altered disposition and gene expression of pDNA...
  66. ncbi request reprint The uptake and degradation of DNA is impaired in macrophages and dendritic cells from NZB/W F(1) mice
    Yoshiyuki Ogawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Immunol Lett 101:32-40. 2005
    ..Hence, the present results imply an impaired function of lupus MPhis and DCs of NZB/W mice to cause retained DNA clearance...
  67. ncbi request reprint [Development of heat shock proteins with controlled distribution properties and their application to vaccine delivery]
    Makiya Nishikawa
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    Yakugaku Zasshi 127:293-300. 2007
    ..These findings indicate that effective cancer therapy can be achieved by developing Hsp70-based anticancer vaccines when their tissue and intracellular distribution is properly controlled...
  68. ncbi request reprint Heat shock protein derivatives for delivery of antigens to antigen presenting cells
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Int J Pharm 354:23-7. 2008
    ..These findings indicate that effective cancer therapy can be achieved by developing Hsp70-based anticancer vaccines when their tissue and intracellular distribution is properly controlled...
  69. doi request reprint Control of hypoxia-induced tumor cell adhesion by cytophilic human catalase
    Tomoya Yata
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Science, Kyoto University, Sakyo ku, Kyoto 606 8501, Sakyo ku, Japan
    Free Radic Biol Med 47:1772-8. 2009
    ..hCAT-(RGD)3 or hCAT-R9 efficiently inhibited these changes compared with hCAT. These results indicate that cytophilic hCAT-(RGD)3 and hCAT-R9 are effective in inhibiting hypoxia-induced tumor cell adhesion to endothelial cells...
  70. doi request reprint Increase in CpG DNA-induced inflammatory responses by DNA oxidation in macrophages and mice
    Hiroyuki Yoshida
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshidashimoadachi cho 46 29, Sakyo ku, Kyoto 606 8501, Japan
    Free Radic Biol Med 51:424-31. 2011
    ..These results indicate that oxo-dG-containing DNA increases the CpG motif-dependent inflammatory responses, which would exacerbate DNA-related autoimmune diseases...
  71. doi request reprint Near-infrared fluorescence probes for enzymes based on binding affinity modulation of squarylium dye scaffold
    Daihi Oushiki
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Anal Chem 84:4404-10. 2012
    ..Our strategy should be applicable to obtain NIR fluorescence probes for a wide range of target enzymes...
  72. ncbi request reprint Pharmacokinetics and preventive effects of targeted catalase derivatives on hydrogen peroxide-induced injury in perfused rat liver
    Yoshiyuki Yabe
    Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Pharm Res 19:1815-21. 2002
    ..To investigate the pharmacokinetics and preventive effects of liver-targeted catalase (CAT) derivatives on hepatic injury caused by reactive oxygen species...
  73. ncbi request reprint Theoretical considerations involving the pharmacokinetics of plasmid DNA
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Adv Drug Deliv Rev 57:675-88. 2005
    ..Here we discuss the pharmacokinetic evaluation of the tissue distribution characteristics of plasmid DNA, in the free and complexed forms...
  74. ncbi request reprint Plasmid DNA uptake and subsequent cellular activation characteristics in human monocyte-derived cells in primary culture
    Yuga Fukuhara
    Department of Biopharmaceutics and Drug Metabolism, Graduated School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Pharm Sci 96:1576-84. 2007
    ..These results indicate that, among human monocyte-derived cells, macrophages are activated by DNA when complexed with cationic liposomes in a CpG motif-independent manner...
  75. doi request reprint Gene silencing of beta-catenin in melanoma cells retards their growth but promotes the formation of pulmonary metastasis in mice
    Yuki Takahashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    Int J Cancer 123:2315-20. 2008
    ..These results raise concerns about the gene silencing of beta-catenin for inhibiting tumor growth, because it promotes tumor metastasis by reducing the amount of cadherin in tumor cells...
  76. doi request reprint Nonviral vector-mediated RNA interference: its gene silencing characteristics and important factors to achieve RNAi-based gene therapy
    Yuki Takahashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, 606 8501, Japan
    Adv Drug Deliv Rev 61:760-6. 2009
    ..Recent progresses in prolonging the RNAi effect, improving the delivery to target cells and increasing the specificity of RNAi in vivo are also reviewed...
  77. doi request reprint DNA-based nano-sized systems for pharmaceutical and biomedical applications
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Adv Drug Deliv Rev 62:626-32. 2010
    ....
  78. ncbi request reprint Residualizing indium-111-radiolabel for plasmid DNA and its application to tissue distribution study
    Makiya Nishikawa
    Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    Bioconjug Chem 14:955-61. 2003
    ..These results indicate that the novel residualizing radiolabel clearly demonstrates the cells that have taken up pDNA and, therefore, gives us useful information about how to design a better approach for nonviral in vivo gene delivery...
  79. ncbi request reprint The hydrodynamics-based procedure for controlling the pharmacokinetics of gene medicines at whole body, organ and cellular levels
    Naoki Kobayashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Adv Drug Deliv Rev 57:713-31. 2005
    ....
  80. ncbi request reprint [In vivo siRNA delivery to tumor cells and its application to cancer gene therapy]
    Yuki Takahashi
    Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshidashimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Yakugaku Zasshi 127:1525-31. 2007
    ..These results indicate the possibility of RNAi-based cancer therapy...
  81. ncbi request reprint Catalase delivery for inhibiting ROS-mediated tissue injury and tumor metastasis
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Kyoto University, Kyoto, Japan
    Adv Drug Deliv Rev 61:319-26. 2009
    ..Because oxygen plays a fundamental key role in our life and oxidative stress is implicated in a wide variety of human diseases, catalase delivery could have wide application in the near future...
  82. doi request reprint The assembly of a short linear natural cytosine-phosphate-guanine DNA into dendritic structures and its effect on immunostimulatory activity
    Sakulrat Rattanakiat
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biomaterials 30:5701-6. 2009
    ..These results suggest that the dendritic structure formation is a potential approach to increasing the immunostimulatory activity of CpG DNA without any modifications of the chemical structure of the natural phosphodiester DNA...
  83. ncbi request reprint An evaluation method for nonlinear local disposition in rat liver and kidney
    Takako Kato
    Department of Biopharmaceutics and Drug Metabolism, School of Graduate Pharmaceutical Science, Kyoto University, Kyoto 606 8501, Japan
    Drug Metab Dispos 32:230-4. 2004
    ..The present local pharmacokinetic method would be applicable to assess the local disposition of other drugs that are mainly metabolized in these organs...
  84. ncbi request reprint Therapeutic effect of intravenous interferon gene delivery with naked plasmid DNA in murine metastasis models
    Naoki Kobayashi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    Mol Ther 6:737-44. 2002
    ..These results suggest that the hydrodynamics-based transfer of naked pDNA is a convenient and efficient method of IFN gene therapy against metastatic tumors...
  85. ncbi request reprint Inhibition of experimental hepatic metastasis by targeted delivery of catalase in mice
    Makiya Nishikawa
    Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    Clin Exp Metastasis 21:213-21. 2004
    ..Decreased MMP activity by catalase delivery seems to be involved in its anti-metastatic effect...
  86. ncbi request reprint Analysis methods and recent advances in nonlinear pharmacokinetics from in vitro through in loci to in vivo
    Kiyoshi Yamaoka
    Department of Biopharmaceutics and Drug Metabolism, School of Graduate Pharmaceutical Science, Kyoto University, Japan
    Drug Metab Pharmacokinet 19:397-406. 2004
    ..The nonlinear global pharmacokinetics in vivo is reviewed with regard to absorption, elimination (metabolism and excretion) and distribution...
  87. doi request reprint Pharmacokinetic considerations regarding non-viral cancer gene therapy
    Makiya Nishikawa
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Cancer Sci 99:856-62. 2008
    ..Here we review the pharmacokinetics of both non-viral vectors and transgene products, and discuss what should be done to achieve safer and more effective non-viral cancer gene therapy...
  88. ncbi request reprint Disposition and gene expression characteristics in solid tumors and skeletal muscle after direct injection of naked plasmid DNA in mice
    Atsushi Kawase
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pharm Sci 92:1295-304. 2003
    ..These findings provide useful information for direct naked pDNA delivery into solid tumors...
  89. pmc Restricted cytokine production from mouse peritoneal macrophages in culture in spite of extensive uptake of plasmid DNA
    Kei Yasuda
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshida Shimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Immunology 111:282-90. 2004
    ....
  90. ncbi request reprint Electrical charge on protein regulates its absorption from the rat small intestine
    Makiya Nishikawa
    Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Am J Physiol Gastrointest Liver Physiol 282:G711-9. 2002
    ..A similar relationship was observed using superoxide dismutase. These findings indicate that the intestinal absorption of a protein is, at least partially, determined by its electrical charge...
  91. ncbi request reprint Pharmacokinetic analysis of in vivo disposition of succinylated proteins targeted to liver nonparenchymal cells via scavenger receptors: importance of molecular size and negative charge density for in vivo recognition by receptors
    Yasuomi Yamasaki
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Pharmacol Exp Ther 301:467-77. 2002
    ..Thus, the present study has provided useful basic information for a therapeutic strategy and the molecular design of succinylated proteins for use as drug carriers and therapeutic agents per se for SR-mediated targeting in vivo...
  92. ncbi request reprint Influence of physicochemical properties on pharmacokinetics of non-viral vectors for gene delivery
    Yoshinobu Takakura
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
    J Drug Target 10:99-104. 2002
    ..Optimization of the physicochemical properties is important for successful in vivo gene delivery by non-viral vectors...
  93. ncbi request reprint Efficient scavenger receptor-mediated hepatic targeting of proteins by introduction of negative charges on the proteins by aconitylation: the influence of charge density and size of the proteins molecules
    Yasuomi Yamasaki
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Eur J Pharm Sci 18:305-12. 2003
    ....
  94. ncbi request reprint Histogram analysis of pharmacokinetic parameters by bootstrap resampling from one-point sampling data in animal experiments
    Seiji Takemoto
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Science, Kyoto University
    Drug Metab Pharmacokinet 21:458-64. 2006
    ..In conclusion, the bootstrap method was found to be an efficient method for assessing the histogram of pharmacokinetic parameters of blood or tissue disposition data by one-point sampling...
  95. ncbi request reprint Role of immunostimulatory DNA and TLR9 in gene therapy
    Kei Yasuda
    Renal Section, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
    Crit Rev Ther Drug Carrier Syst 23:89-110. 2006
    ..Moreover, recent research shows that TLR9-independent immunoactivation could take place. Here, we have attempted to present an overview of immunoactivation by DNA to optimize DNA-based therapies...
  96. ncbi request reprint Plasmid DNA activates murine macrophages to induce inflammatory cytokines in a CpG motif-independent manner by complex formation with cationic liposomes
    Kei Yasuda
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29, Yoshidashimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Biochem Biophys Res Commun 293:344-8. 2002
    ..Taken together, the present study demonstrated that for the first time DNA can stimulate murine macrophages in a CpG motif-independent manner when it is complexed with the cationic liposomes...
  97. ncbi request reprint Important role of serum proteins associated on the surface of particles in their hepatic disposition
    Kentaro Furumoto
    Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University, 1 1 1 Tsushima naka, Okayama 700 8530, Japan
    J Control Release 83:89-96. 2002
    ....
  98. ncbi request reprint Efficient uptake and rapid degradation of plasmid DNA by murine dendritic cells via a specific mechanism
    Takaharu Yoshinaga
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshidashimoadachi cho, Sakyo ku, Kyoto, Japan
    Biochem Biophys Res Commun 299:389-94. 2002
    ..These findings may suggest the important role of the dendritic cells in the innate immune system for host defense...
  99. ncbi request reprint Effect of interferon-gamma on the pharmacokinetics of digoxin, a P-glycoprotein substrate, intravenously injected into the mouse
    Hiroko Kawaguchi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Pharmacol Exp Ther 308:91-6. 2004
    ..Thus, a more pronounced effect may be elicited by IFN-gamma for common substrates of P-gp and CYP3A...
  100. ncbi request reprint Expression and visualization of a human interferon-beta-enhanced green fluorescent protein chimeric molecule in cultured cells
    Masaru Nakamura
    Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Biol Pharm Bull 27:411-4. 2004
    ..These results suggest that HuIFN-beta-EGFP will be a useful tool to investigate the intracellular trafficking processes of HuIFN-beta in a variety of cell types...
  101. pmc Efficient scavenger receptor-mediated uptake and cross-presentation of negatively charged soluble antigens by dendritic cells
    Kohsuke Shakushiro
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Immunology 112:211-8. 2004
    ..Therefore, SR-mediated delivery of antigen to DC leads to efficient cross-presentation, although the pathway of chemical modification should be considered...