Affiliation: Kyoto University
- Kanatsu Shinohara M, Morimoto H, Shinohara T. Enrichment of Mouse Spermatogonial Stem Cells by the Stem Cell Dye CDy1. Biol Reprod. 2016;94:13 pubmed publisher..Thus, CDy1 staining will provide a useful strategy for the enrichment of SSCs and may be used in conjunction with other reagents for the enrichment of SSCs. ..
- Kanatsu Shinohara M, Morimoto H, Shinohara T. Fertility of Male Germline Stem Cells Following Spermatogonial Transplantation in Infertile Mouse Models. Biol Reprod. 2016;94:112 pubmed publisher..Efficient offspring production using GS cells and spermatogonial transplantation will be useful for analyzing factors involved in male fertility. ..
- Morimoto H, Kanatsu Shinohara M, Shinohara T. ROS-Generating Oxidase Nox3 Regulates the Self-Renewal of Mouse Spermatogonial Stem Cells. Biol Reprod. 2015;92:147 pubmed publisher..Our results suggest that self-renewal of SSCs is regulated by sequential activation of different Nox genes, and underscore the complexity of ROS regulation in the self-renewal division of SSCs. ..
- Tanaka T, Kanatsu Shinohara M, Lei Z, Rao C, Shinohara T. The Luteinizing Hormone-Testosterone Pathway Regulates Mouse Spermatogonial Stem Cell Self-Renewal by Suppressing WNT5A Expression in Sertoli Cells. Stem Cell Reports. 2016;7:279-91 pubmed publisher..WNT5A promoted SSC activity both in vitro and in vivo. Therefore, FSH is not responsible for GDNF regulation, while LH negatively regulates SSC self-renewal by suppressing WNT5A via testosterone. ..