David Lembo

Summary

Affiliation: University of Torino
Location: Torino, Italy
Summary:
More than 20 years experience in Virology with focus on virus-cell interactions and research and development of antivirals.
Currently heading the Laboratory of Molecular Virology at the University of Turin, Italy

The biographical details of Prof. D. Lembo have been included in the 2011 and 2012 Edition of "Marquis Whos Who in the World".

He is the author or co-author of more than 50 publications in peer-reviewed international journals.

He is inventor of several patents in the field of antivirals and nanomedicine.

In 2005 he was awarded the “Technology Scouting in Academia” prize.

He is a scientific consultant of several biotech companies.

He is co-founder of the biotech company RotaLactis.

Research Interests
• Studies on virus-cell interactions.
• Biochemical and functional characterization of viral proteins with a focus on herpesviruses and papillomaviruses.
• Research and development of antiviral molecules with a focus on herpesviruses, papillomaviruses, rotaviruses and respiratory syncytial virus.
• Research and development of innovative formulations based on nanocarriers for the delivery of antiviral and antibacterial drugs, peptides, enzymes and nucleic acids

Viruses routinely screened for antiviral drug discovery in my lab:
- Herpes simplex virus
- Cytomegalovirus
- Human papillomaviruses (pseudovirus neutralization assay)
- Rotaviruses
- Respiratory syncytial virus

Publications

  1. doi Preparation and characterization of dextran nanobubbles for oxygen delivery
    R Cavalli
    Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino Via Pietro Giuria 9 10125, Torino, Italy
    Int J Pharm 381:160-5. 2009
  2. pmc Inhibition of human respiratory syncytial virus infectivity by a dendrimeric heparan sulfate-binding peptide
    Manuela Donalisio
    Department of Clinical and Biological Sciences, University of Turin, S Luigi Gonzaga Medical School, Turin, Italy
    Antimicrob Agents Chemother 56:5278-88. 2012
  3. pmc New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization
    Roberta Cavalli
    Department of Pharmaceutical Sciences and Technology, University of Turin, Turin, Italy
    Int J Nanomedicine 7:3309-18. 2012
  4. doi Enhanced antiviral activity of acyclovir loaded into nanoparticles
    Roberta Cavalli
    Dipartimento di Scienza e Tecnologia del Farmaco, Universita di Torino, Torino, Italy
    Methods Enzymol 509:1-19. 2012
  5. pmc Poly(amidoamine)-Cholesterol Conjugate Nanoparticles Obtained by Electrospraying as Novel Tamoxifen Delivery System
    R Cavalli
    Dipartimento di Scienza e Tecnologia del Farmaco, Universita di Torino, via P Giuria 9, 10125 Torino, Italy
    J Drug Deliv 2011:587604. 2011
  6. doi Early inhibitors of human cytomegalovirus: state-of-art and therapeutic perspectives
    Beatrice Mercorelli
    Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, 35121 Padova, Italy
    Pharmacol Ther 131:309-29. 2011
  7. doi Nanoparticulate delivery systems for antiviral drugs
    David Lembo
    Dipartimento di Scienze Cliniche e Biologiche, Universita degli Studi di Torino, Orbassano Torino, Italy
    Antivir Chem Chemother 21:53-70. 2010
  8. doi Putative mechanisms of antitumor activity of cyano-substituted heteroaryles in HeLa cells
    Katja Ester
    Division of Molecular Medicine, Ruđer Bošković Institute, Bijenicka 54, HR 10000 Zagreb, Croatia
    Invest New Drugs 30:450-67. 2012
  9. pmc Sulfated derivatives of Escherichia coli K5 capsular polysaccharide are potent inhibitors of human cytomegalovirus
    Beatrice Mercorelli
    Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via Gabelli 63, Padua, Italy
    Antimicrob Agents Chemother 54:4561-7. 2010
  10. pmc Identification of a dendrimeric heparan sulfate-binding peptide that inhibits infectivity of genital types of human papillomaviruses
    Manuela Donalisio
    Department of Clinical and Biological Sciences, University of Turin, S Luigi Gonzaga Hospital, Regione Gonzole 10, 10043 Orbassano, Turin, Italy
    Antimicrob Agents Chemother 54:4290-9. 2010

Collaborators

Detail Information

Publications27

  1. doi Preparation and characterization of dextran nanobubbles for oxygen delivery
    R Cavalli
    Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino Via Pietro Giuria 9 10125, Torino, Italy
    Int J Pharm 381:160-5. 2009
    ..The oxygen release kinetics could be enhanced after nanobubble insonation with ultrasound at 2.5MHz. The oxygen-filled nanobubble formulations might be proposed for therapeutic applications in various diseases...
  2. pmc Inhibition of human respiratory syncytial virus infectivity by a dendrimeric heparan sulfate-binding peptide
    Manuela Donalisio
    Department of Clinical and Biological Sciences, University of Turin, S Luigi Gonzaga Medical School, Turin, Italy
    Antimicrob Agents Chemother 56:5278-88. 2012
    ..Together, these features render SB105-A10 an attractive candidate for further development as a RSV inhibitor to be administered by aerosol delivery...
  3. pmc New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization
    Roberta Cavalli
    Department of Pharmaceutical Sciences and Technology, University of Turin, Turin, Italy
    Int J Nanomedicine 7:3309-18. 2012
    ..In this study, we describe the development of a novel chitosan nanobubble-based gene delivery system for ultrasound-triggered release...
  4. doi Enhanced antiviral activity of acyclovir loaded into nanoparticles
    Roberta Cavalli
    Dipartimento di Scienza e Tecnologia del Farmaco, Universita di Torino, Torino, Italy
    Methods Enzymol 509:1-19. 2012
    ..The biocompatibility assays necessary to obtain safe nanoparticles are reported. Section 4 of this chapter describes the assessment of the antiviral activity of the acyclovir-loaded nanoparticles...
  5. pmc Poly(amidoamine)-Cholesterol Conjugate Nanoparticles Obtained by Electrospraying as Novel Tamoxifen Delivery System
    R Cavalli
    Dipartimento di Scienza e Tecnologia del Farmaco, Universita di Torino, via P Giuria 9, 10125 Torino, Italy
    J Drug Deliv 2011:587604. 2011
    ..The electrospray technique might be proposed to produce tamoxifen-loaded PAA-chol nanoparticle in powder form without any excipient in a single step...
  6. doi Early inhibitors of human cytomegalovirus: state-of-art and therapeutic perspectives
    Beatrice Mercorelli
    Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, 35121 Padova, Italy
    Pharmacol Ther 131:309-29. 2011
    ..This review describes the current knowledge on the initial phases of HCMV replication, their validation as potential novel antiviral targets, and the development of compounds that block such processes...
  7. doi Nanoparticulate delivery systems for antiviral drugs
    David Lembo
    Dipartimento di Scienze Cliniche e Biologiche, Universita degli Studi di Torino, Orbassano Torino, Italy
    Antivir Chem Chemother 21:53-70. 2010
    ..The paper closes with a discussion on the future challenges that must be addressed before the potential of nanotechnology can be translated into safe and effective antiviral formulations for clinical use...
  8. doi Putative mechanisms of antitumor activity of cyano-substituted heteroaryles in HeLa cells
    Katja Ester
    Division of Molecular Medicine, Ruđer Bošković Institute, Bijenicka 54, HR 10000 Zagreb, Croatia
    Invest New Drugs 30:450-67. 2012
    ....
  9. pmc Sulfated derivatives of Escherichia coli K5 capsular polysaccharide are potent inhibitors of human cytomegalovirus
    Beatrice Mercorelli
    Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via Gabelli 63, Padua, Italy
    Antimicrob Agents Chemother 54:4561-7. 2010
    ..Interestingly, our studies highlight that small variations of the K5 derivatives structure can modulate the selectivity and potency of their activities against different viruses, including viruses belonging to the same family...
  10. pmc Identification of a dendrimeric heparan sulfate-binding peptide that inhibits infectivity of genital types of human papillomaviruses
    Manuela Donalisio
    Department of Clinical and Biological Sciences, University of Turin, S Luigi Gonzaga Hospital, Regione Gonzole 10, 10043 Orbassano, Turin, Italy
    Antimicrob Agents Chemother 54:4290-9. 2010
    ..The findings from this study indicate SB105-A10 to be a leading candidate compound for further development as an active ingredient of a topical microbicide against HPV and other sexually transmitted viral infections...
  11. doi Effects of cytokines on long control region transcriptional activity in high-risk cutaneous human papillomavirus types 5 and 8
    Manuela Donalisio
    Department of Clinical and Biological Sciences, S Luigi Gonzaga Hospital, University of Turin, Regione Gonzole, 10, 10043 Orbassano, Turin, Italy
    Arch Virol 155:583-7. 2010
    ..This novel assay has allowed the first systematic analysis of the effects of cytokines on HPV-5 and HPV-8 LCR activity and provides a valuable tool for the search for cutaneous HPV-gene expression inhibitors...
  12. doi Sulfated K5 Escherichia coli polysaccharide derivatives: A novel class of candidate antiviral microbicides
    Marco Rusnati
    Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, 25123 Brescia, Italy
    Pharmacol Ther 123:310-22. 2009
    ..With a focus on the K5 derivatives, this article reviews the literature on polyanions as antiviral microbicides and discusses the possible therapeutic implications of this novel class of compounds...
  13. doi Enhanced antiviral activity of Acyclovir loaded into beta-cyclodextrin-poly(4-acryloylmorpholine) conjugate nanoparticles
    Roberta Cavalli
    Dipartimento di Scienza e Tecnologia del Farmaco, Universita degli Studi di Torino, via P Giuria 9, 10125 Torino, Italy
    J Control Release 137:116-22. 2009
    ..It was found that the nanoparticles are internalized in cells and locate in the perinuclear compartment...
  14. doi Tinkering with a viral ribonucleotide reductase
    David Lembo
    Department of Clinical and Biological Sciences, University of Turin, S Luigi Gonzaga Hospital, Orbassano, Turin, Italy
    Trends Biochem Sci 34:25-32. 2009
    ..The discovery of this novel mechanism of viral immune subversion provides further support to the concept of evolutionary tinkering...
  15. pmc Inhibition of proinflammatory and innate immune signaling pathways by a cytomegalovirus RIP1-interacting protein
    Claudia Mack
    Division of Viral Infections, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
    Proc Natl Acad Sci U S A 105:3094-9. 2008
    ..The results presented here reveal a mechanism of viral immune subversion and demonstrate how a viral protein can simultaneously block proinflammatory and innate immune signaling pathways by interacting with a central mediator molecule...
  16. pmc Sulfated K5 Escherichia coli polysaccharide derivatives as wide-range inhibitors of genital types of human papillomavirus
    David Lembo
    Department of Clinical and Biological Sciences, University of Turin, San Luigi Gonzaga Hospital, Regione Gonzole 10, 10043 Orbassano, Turin, Italy
    Antimicrob Agents Chemother 52:1374-81. 2008
    ..Their 50% inhibitory concentration was between 0.1 and 0.9 mug/ml, without evidence of cytotoxicity. These findings provide insights into the design of novel, safe, and broad-spectrum microbicides against genital HPV infections...
  17. doi TGF-beta1 and IL-4 downregulate human papillomavirus-16 oncogene expression but have differential effects on the malignant phenotype of cervical carcinoma cells
    Manuela Donalisio
    Department of Clinical and Biological Sciences, University of Turin, 10043 Orbassano, Turin, Italy
    Virus Res 132:253-6. 2008
    ..These findings suggest that the two cytokines may play a role in the control of HPV infections, however, cervical carcinoma cells developed a partial or a total resistance to their inhibitory activity...
  18. ncbi Preparation and in vitro evaluation of the antiviral activity of the Acyclovir complex of a beta-cyclodextrin/poly(amidoamine) copolymer
    Marco Bencini
    Dipartimento di Chimica Organica e Industriale, and Centro Interdisciplinare Materiali e Interfacce Nanostrutturati CIMAINA Università di Milano, via Venezian 21, 20133 Milano, Italy
    J Control Release 126:17-25. 2008
    ..The antiviral activity of Acyclovir beta-CD/PAA complex was evaluated against herpes simplex virus type I in cell cultures. The Acyclovir beta-CD/PAA complex exhibited a higher antiviral activity than the free drug...
  19. doi Encapsulation of Acyclovir in new carboxylated cyclodextrin-based nanosponges improves the agent's antiviral efficacy
    David Lembo
    Dipartimento di Scienze Cliniche e Biologiche, Università degli Studi di Torino Ospedale S Luigi Gonzaga, 10043 Orbassano, Torino, Italy
    Int J Pharm 443:262-72. 2013
    ..The NS uptake into cells was evaluated using fluorescent Carb-NS and revealed the nanoparticle internalisation. Enhanced antiviral activity against a clinical isolate of HSV-1 was obtained using Acyclovir loaded in Carb-NS...
  20. pmc Filoviruses utilize glycosaminoglycans for their attachment to target cells
    Beatriz Salvador
    Blood Systems Research Institute, San Francisco, CA, USA
    J Virol 87:3295-304. 2013
    ....
  21. ncbi The human cytomegalovirus
    Santo Landolfo
    Department of Public Health and Microbiology, University of Turin, Via Santena 9, 10126 Turin, Italy
    Pharmacol Ther 98:269-97. 2003
    ..The presently available drugs produced a significant clinical improvement, but suffer from poor oral bioavailability, low potency, development of resistance in clinical practice, and dose-limiting toxicities...
  22. pmc In vitro screening for antiviral activity of Turkish plants revealing methanolic extract of Rindera lanata var. lanata active against human rotavirus
    Andrea Civra
    Department of Clinical and Biological Sciences, University of Torino, S Luigi Gonzaga Hospital, Regione Gonzole, 10, 10043, Orbassano, Torino, Italy
    BMC Complement Altern Med 17:74. 2017
    ..In this study, we explored medicinal plants endemic to Turkey flora as a source of anti-HRoV compunds...
  23. doi Oxysterols: An emerging class of broad spectrum antiviral effectors
    David Lembo
    Department of Clinical and Biological Sciences, University of Torino at San Luigi Gonzaga Hospital, 10043 Orbassano, Torino, Italy Electronic address
    Mol Aspects Med 49:23-30. 2016
    ..In the present paper, we review the recent findings about the broad antiviral activity of oxysterols against enveloped and non-enveloped human viral pathogens, and provide an overview of their putative antiviral mechnism(s). ..
  24. pmc In vitro anti-Herpes simplex virus activity of crude extract of the roots of Nauclea latifolia Smith (Rubiaceae)
    Manuela Donalisio
    Laboratory of Molecular Virology, Department of Clinical and Biological Sciences, University of Turin, PO Box 10043, Torino, Italy
    BMC Complement Altern Med 13:266. 2013
    ..latifolia. In this study, HSV-2 was chosen as a viral model because of its strong impact on HIV transmission and acquisition...
  25. ncbi Effect of high-risk human papillomavirus oncoproteins on p53R2 gene expression after DNA damage
    David Lembo
    Department of Public Health and Microbiology, University of Turin, Via Santena 9, 10126 Turin, Italy
    Virus Res 122:189-93. 2006
    ....
  26. ncbi A cell-based high-throughput assay for screening inhibitors of human papillomavirus-16 long control region activity
    David Lembo
    Department of Public Health and Microbiology, University of Turin, Italy
    FASEB J 20:148-50. 2006
    ..Our assay provides a validated tool in the search for drugs against HPV-associated cervical carcinomas and allowed the first systematic analysis of the effect of cytokines on the HPV-16 LCR transcriptional activity...
  27. pmc The ribonucleotide reductase R1 homolog of murine cytomegalovirus is not a functional enzyme subunit but is required for pathogenesis
    David Lembo
    Department of Public Health and Microbiology, University of Turin, Turin, Italy
    J Virol 78:4278-88. 2004
    ..These results suggest that M45 has evolved a new function that is indispensable for virus replication and pathogenesis in vivo...