Affiliation: University of Torino
Location: Torino, Italy
More than 20 years experience in Virology with focus on virus-cell interactions and research and development of antivirals.
Currently heading the Laboratory of Molecular Virology at the University of Turin, Italy
The biographical details of Prof. D. Lembo have been included in the 2011 and 2012 Edition of "Marquis Whos Who in the World".
He is the author or co-author of more than 50 publications in peer-reviewed international journals.
He is inventor of several patents in the field of antivirals and nanomedicine.
In 2005 he was awarded the “Technology Scouting in Academia” prize.
He is a scientific consultant of several biotech companies.
He is co-founder of the biotech company RotaLactis.
• Studies on virus-cell interactions.
• Biochemical and functional characterization of viral proteins with a focus on herpesviruses and papillomaviruses.
• Research and development of antiviral molecules with a focus on herpesviruses, papillomaviruses, rotaviruses and respiratory syncytial virus.
• Research and development of innovative formulations based on nanocarriers for the delivery of antiviral and antibacterial drugs, peptides, enzymes and nucleic acids
Viruses routinely screened for antiviral drug discovery in my lab:
- Herpes simplex virus
- Human papillomaviruses (pseudovirus neutralization assay)
- Respiratory syncytial virus
- Preparation and characterization of dextran nanobubbles for oxygen deliveryR Cavalli
Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino Via Pietro Giuria 9 10125, Torino, Italy
Int J Pharm 381:160-5. 2009..The oxygen release kinetics could be enhanced after nanobubble insonation with ultrasound at 2.5MHz. The oxygen-filled nanobubble formulations might be proposed for therapeutic applications in various diseases...
- Inhibition of human respiratory syncytial virus infectivity by a dendrimeric heparan sulfate-binding peptideManuela Donalisio
Department of Clinical and Biological Sciences, University of Turin, S Luigi Gonzaga Medical School, Turin, Italy
Antimicrob Agents Chemother 56:5278-88. 2012..Together, these features render SB105-A10 an attractive candidate for further development as a RSV inhibitor to be administered by aerosol delivery...
- New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterizationRoberta Cavalli
Department of Pharmaceutical Sciences and Technology, University of Turin, Turin, Italy
Int J Nanomedicine 7:3309-18. 2012..In this study, we describe the development of a novel chitosan nanobubble-based gene delivery system for ultrasound-triggered release...
- Enhanced antiviral activity of acyclovir loaded into nanoparticlesRoberta Cavalli
Dipartimento di Scienza e Tecnologia del Farmaco, Universita di Torino, Torino, Italy
Methods Enzymol 509:1-19. 2012..The biocompatibility assays necessary to obtain safe nanoparticles are reported. Section 4 of this chapter describes the assessment of the antiviral activity of the acyclovir-loaded nanoparticles...
- Poly(amidoamine)-Cholesterol Conjugate Nanoparticles Obtained by Electrospraying as Novel Tamoxifen Delivery SystemR Cavalli
Dipartimento di Scienza e Tecnologia del Farmaco, Universita di Torino, via P Giuria 9, 10125 Torino, Italy
J Drug Deliv 2011:587604. 2011..The electrospray technique might be proposed to produce tamoxifen-loaded PAA-chol nanoparticle in powder form without any excipient in a single step...
- Early inhibitors of human cytomegalovirus: state-of-art and therapeutic perspectivesBeatrice Mercorelli
Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, 35121 Padova, Italy
Pharmacol Ther 131:309-29. 2011..This review describes the current knowledge on the initial phases of HCMV replication, their validation as potential novel antiviral targets, and the development of compounds that block such processes...
- Nanoparticulate delivery systems for antiviral drugsDavid Lembo
Dipartimento di Scienze Cliniche e Biologiche, Universita degli Studi di Torino, Orbassano Torino, Italy
Antivir Chem Chemother 21:53-70. 2010..The paper closes with a discussion on the future challenges that must be addressed before the potential of nanotechnology can be translated into safe and effective antiviral formulations for clinical use...
- Putative mechanisms of antitumor activity of cyano-substituted heteroaryles in HeLa cellsKatja Ester
Division of Molecular Medicine, Ruđer Bošković Institute, Bijenicka 54, HR 10000 Zagreb, Croatia
Invest New Drugs 30:450-67. 2012....
- Sulfated derivatives of Escherichia coli K5 capsular polysaccharide are potent inhibitors of human cytomegalovirusBeatrice Mercorelli
Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via Gabelli 63, Padua, Italy
Antimicrob Agents Chemother 54:4561-7. 2010..Interestingly, our studies highlight that small variations of the K5 derivatives structure can modulate the selectivity and potency of their activities against different viruses, including viruses belonging to the same family...
- Identification of a dendrimeric heparan sulfate-binding peptide that inhibits infectivity of genital types of human papillomavirusesManuela Donalisio
Department of Clinical and Biological Sciences, University of Turin, S Luigi Gonzaga Hospital, Regione Gonzole 10, 10043 Orbassano, Turin, Italy
Antimicrob Agents Chemother 54:4290-9. 2010..The findings from this study indicate SB105-A10 to be a leading candidate compound for further development as an active ingredient of a topical microbicide against HPV and other sexually transmitted viral infections...
- Effects of cytokines on long control region transcriptional activity in high-risk cutaneous human papillomavirus types 5 and 8Manuela Donalisio
Department of Clinical and Biological Sciences, S Luigi Gonzaga Hospital, University of Turin, Regione Gonzole, 10, 10043 Orbassano, Turin, Italy
Arch Virol 155:583-7. 2010..This novel assay has allowed the first systematic analysis of the effects of cytokines on HPV-5 and HPV-8 LCR activity and provides a valuable tool for the search for cutaneous HPV-gene expression inhibitors...
- Sulfated K5 Escherichia coli polysaccharide derivatives: A novel class of candidate antiviral microbicidesMarco Rusnati
Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, 25123 Brescia, Italy
Pharmacol Ther 123:310-22. 2009..With a focus on the K5 derivatives, this article reviews the literature on polyanions as antiviral microbicides and discusses the possible therapeutic implications of this novel class of compounds...
- Enhanced antiviral activity of Acyclovir loaded into beta-cyclodextrin-poly(4-acryloylmorpholine) conjugate nanoparticlesRoberta Cavalli
Dipartimento di Scienza e Tecnologia del Farmaco, Universita degli Studi di Torino, via P Giuria 9, 10125 Torino, Italy
J Control Release 137:116-22. 2009..It was found that the nanoparticles are internalized in cells and locate in the perinuclear compartment...
- Tinkering with a viral ribonucleotide reductaseDavid Lembo
Department of Clinical and Biological Sciences, University of Turin, S Luigi Gonzaga Hospital, Orbassano, Turin, Italy
Trends Biochem Sci 34:25-32. 2009..The discovery of this novel mechanism of viral immune subversion provides further support to the concept of evolutionary tinkering...
- Inhibition of proinflammatory and innate immune signaling pathways by a cytomegalovirus RIP1-interacting proteinClaudia Mack
Division of Viral Infections, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
Proc Natl Acad Sci U S A 105:3094-9. 2008..The results presented here reveal a mechanism of viral immune subversion and demonstrate how a viral protein can simultaneously block proinflammatory and innate immune signaling pathways by interacting with a central mediator molecule...
- Sulfated K5 Escherichia coli polysaccharide derivatives as wide-range inhibitors of genital types of human papillomavirusDavid Lembo
Department of Clinical and Biological Sciences, University of Turin, San Luigi Gonzaga Hospital, Regione Gonzole 10, 10043 Orbassano, Turin, Italy
Antimicrob Agents Chemother 52:1374-81. 2008..Their 50% inhibitory concentration was between 0.1 and 0.9 mug/ml, without evidence of cytotoxicity. These findings provide insights into the design of novel, safe, and broad-spectrum microbicides against genital HPV infections...
- TGF-beta1 and IL-4 downregulate human papillomavirus-16 oncogene expression but have differential effects on the malignant phenotype of cervical carcinoma cellsManuela Donalisio
Department of Clinical and Biological Sciences, University of Turin, 10043 Orbassano, Turin, Italy
Virus Res 132:253-6. 2008..These findings suggest that the two cytokines may play a role in the control of HPV infections, however, cervical carcinoma cells developed a partial or a total resistance to their inhibitory activity...
- Preparation and in vitro evaluation of the antiviral activity of the Acyclovir complex of a beta-cyclodextrin/poly(amidoamine) copolymerMarco Bencini
Dipartimento di Chimica Organica e Industriale, and Centro Interdisciplinare Materiali e Interfacce Nanostrutturati CIMAINA Università di Milano, via Venezian 21, 20133 Milano, Italy
J Control Release 126:17-25. 2008..The antiviral activity of Acyclovir beta-CD/PAA complex was evaluated against herpes simplex virus type I in cell cultures. The Acyclovir beta-CD/PAA complex exhibited a higher antiviral activity than the free drug...
- Encapsulation of Acyclovir in new carboxylated cyclodextrin-based nanosponges improves the agent's antiviral efficacyDavid Lembo
Dipartimento di Scienze Cliniche e Biologiche, Università degli Studi di Torino Ospedale S Luigi Gonzaga, 10043 Orbassano, Torino, Italy
Int J Pharm 443:262-72. 2013..The NS uptake into cells was evaluated using fluorescent Carb-NS and revealed the nanoparticle internalisation. Enhanced antiviral activity against a clinical isolate of HSV-1 was obtained using Acyclovir loaded in Carb-NS...
- Filoviruses utilize glycosaminoglycans for their attachment to target cellsBeatriz Salvador
Blood Systems Research Institute, San Francisco, CA, USA
J Virol 87:3295-304. 2013....
- The human cytomegalovirusSanto Landolfo
Department of Public Health and Microbiology, University of Turin, Via Santena 9, 10126 Turin, Italy
Pharmacol Ther 98:269-97. 2003..The presently available drugs produced a significant clinical improvement, but suffer from poor oral bioavailability, low potency, development of resistance in clinical practice, and dose-limiting toxicities...
- In vitro screening for antiviral activity of Turkish plants revealing methanolic extract of Rindera lanata var. lanata active against human rotavirusAndrea Civra
Department of Clinical and Biological Sciences, University of Torino, S Luigi Gonzaga Hospital, Regione Gonzole, 10, 10043, Orbassano, Torino, Italy
BMC Complement Altern Med 17:74. 2017..In this study, we explored medicinal plants endemic to Turkey flora as a source of anti-HRoV compunds...
- Oxysterols: An emerging class of broad spectrum antiviral effectorsDavid Lembo
Department of Clinical and Biological Sciences, University of Torino at San Luigi Gonzaga Hospital, 10043 Orbassano, Torino, Italy Electronic address
Mol Aspects Med 49:23-30. 2016..In the present paper, we review the recent findings about the broad antiviral activity of oxysterols against enveloped and non-enveloped human viral pathogens, and provide an overview of their putative antiviral mechnism(s). ..
- In vitro anti-Herpes simplex virus activity of crude extract of the roots of Nauclea latifolia Smith (Rubiaceae)Manuela Donalisio
Laboratory of Molecular Virology, Department of Clinical and Biological Sciences, University of Turin, PO Box 10043, Torino, Italy
BMC Complement Altern Med 13:266. 2013..latifolia. In this study, HSV-2 was chosen as a viral model because of its strong impact on HIV transmission and acquisition...
- Effect of high-risk human papillomavirus oncoproteins on p53R2 gene expression after DNA damageDavid Lembo
Department of Public Health and Microbiology, University of Turin, Via Santena 9, 10126 Turin, Italy
Virus Res 122:189-93. 2006....
- A cell-based high-throughput assay for screening inhibitors of human papillomavirus-16 long control region activityDavid Lembo
Department of Public Health and Microbiology, University of Turin, Italy
FASEB J 20:148-50. 2006..Our assay provides a validated tool in the search for drugs against HPV-associated cervical carcinomas and allowed the first systematic analysis of the effect of cytokines on the HPV-16 LCR transcriptional activity...
- The ribonucleotide reductase R1 homolog of murine cytomegalovirus is not a functional enzyme subunit but is required for pathogenesisDavid Lembo
Department of Public Health and Microbiology, University of Turin, Turin, Italy
J Virol 78:4278-88. 2004..These results suggest that M45 has evolved a new function that is indispensable for virus replication and pathogenesis in vivo...