Jian He Xu

Summary

Affiliation: East China University of Science and Technology
Country: China

Publications

  1. doi request reprint Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor
    Li Juan Wang
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 102:7023-8. 2011
  2. doi request reprint Enhancing transglutaminase production of Streptomyces mobaraensis by iterative mutagenesis breeding with atmospheric and room-temperature plasma (ARTP)
    Ying Jiang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237 People s Republic of China
    Bioresour Bioprocess 4:37. 2017
  3. ncbi request reprint Assembly of a three-dimensional array of glycoconjugates by combinatorial biocatalysis in nonaqueous media
    Hui Lei Yu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P R China
    J Comb Chem 10:79-87. 2008
  4. doi request reprint Expression and characterization of a novel enantioselective lipase from Aspergillus fumigatus
    Jiao Jiao Shangguan
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 168:1820-33. 2012
  5. doi request reprint Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. ECU0401 and its potential for (R)-(-)-mandelic acid production
    Zhi Jun Zhang
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
    Bioprocess Biosyst Eng 34:315-22. 2011
  6. doi request reprint Significantly improved expression and biochemical properties of recombinant Serratia marcescens lipase as robust biocatalyst for kinetic resolution of chiral ester
    Yi Wang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 162:2387-99. 2010
  7. doi request reprint Expression and characterization of a novel lipase from Aspergillus fumigatus with high specific activity
    Jiao Jiao Shangguan
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 165:949-62. 2011
  8. doi request reprint Resolution of racemic sulfoxides with high productivity and enantioselectivity by a Rhodococcus sp. strain as an alternative to biooxidation of prochiral sulfides for efficient production of enantiopure sulfoxides
    Ai Tao Li
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 102:1537-42. 2011
  9. doi request reprint A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: catalytic properties and performance in kinetic resolution of α-hydroxy acids
    Bao Di Ma
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 133:354-60. 2013
  10. doi request reprint Synthesis of optically pure S-sulfoxide by Escherichia coli transformant cells coexpressing the P450 monooxygenase and glucose dehydrogenase genes
    Jian Dong Zhang
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People s Republic of China
    J Ind Microbiol Biotechnol 38:633-41. 2011

Collaborators

Detail Information

Publications78

  1. doi request reprint Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor
    Li Juan Wang
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 102:7023-8. 2011
    ..These results indicate the potential of ScCR for the industrial production of valuable chiral alcohols...
  2. doi request reprint Enhancing transglutaminase production of Streptomyces mobaraensis by iterative mutagenesis breeding with atmospheric and room-temperature plasma (ARTP)
    Ying Jiang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237 People s Republic of China
    Bioresour Bioprocess 4:37. 2017
    ..mobaraensis mutants with increased TGase production...
  3. ncbi request reprint Assembly of a three-dimensional array of glycoconjugates by combinatorial biocatalysis in nonaqueous media
    Hui Lei Yu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P R China
    J Comb Chem 10:79-87. 2008
    ..And also, the distribution of the artificial array can be adjusted by changing the molar ratio of the auxiliary materials...
  4. doi request reprint Expression and characterization of a novel enantioselective lipase from Aspergillus fumigatus
    Jiao Jiao Shangguan
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 168:1820-33. 2012
    ....
  5. doi request reprint Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. ECU0401 and its potential for (R)-(-)-mandelic acid production
    Zhi Jun Zhang
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
    Bioprocess Biosyst Eng 34:315-22. 2011
    ..The extreme stability, high activity and enantioselectivity of this nitrilase provide a solid base for its practical application in the production of (R)-(-)-mandelic acid...
  6. doi request reprint Significantly improved expression and biochemical properties of recombinant Serratia marcescens lipase as robust biocatalyst for kinetic resolution of chiral ester
    Yi Wang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 162:2387-99. 2010
    ..24 mmol min⁻¹  mg⁻¹, respectively. The above-mentioned features make the highly enantioselective lipase from Serratia marcescens ECU1010 a robust biocatalyst for practical use in large-scale production of diltiazem intermediate...
  7. doi request reprint Expression and characterization of a novel lipase from Aspergillus fumigatus with high specific activity
    Jiao Jiao Shangguan
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 165:949-62. 2011
    ..Ca(2+) and other metal ions could not activate the lipase. According to the homology analysis and site-directed mutagenesis assay, the catalytic triad of the recombinant lipase was identified as Ser-165, Asp-260, and His-290 residues...
  8. doi request reprint Resolution of racemic sulfoxides with high productivity and enantioselectivity by a Rhodococcus sp. strain as an alternative to biooxidation of prochiral sulfides for efficient production of enantiopure sulfoxides
    Ai Tao Li
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 102:1537-42. 2011
    ..7-43.2%; within 1-8 h) and enantioselectivity (ee(S)>99.0%) towards para-substituted (methyl and chloro) phenyl methyl sulfoxides and ethyl phenyl sulfoxide, indicating it could be a promising agent for synthetic applications...
  9. doi request reprint A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: catalytic properties and performance in kinetic resolution of α-hydroxy acids
    Bao Di Ma
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 133:354-60. 2013
    ..The attractive organic-solvent tolerance, good thermostability and high enantioselectivity towards α-acetoxy carboxylates endow rPPE01 with the potential of practical application for the production of enantiopure hydroxy acids...
  10. doi request reprint Synthesis of optically pure S-sulfoxide by Escherichia coli transformant cells coexpressing the P450 monooxygenase and glucose dehydrogenase genes
    Jian Dong Zhang
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People s Republic of China
    J Ind Microbiol Biotechnol 38:633-41. 2011
    ..In this system, the effects of glucose concentration and substrate concentration were further investigated for efficient biotransformation. This system is highly advantageous for the synthesis of optically pure S-sulfoxide...
  11. doi request reprint Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata
    Chen Sheng Zhang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Microbiol Biotechnol 95:91-9. 2012
    ..5%. The development of LaN makes it possible to produce (R)-o-chloromandelic acid by deracemizing o-chloromandelonitrile with good ee value and high substrate concentration...
  12. doi request reprint Cloning of thermostable cellulase genes of Clostridium thermocellum and their secretive expression in Bacillus subtilis
    Jian Ming Liu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
    Appl Biochem Biotechnol 166:652-62. 2012
    ....
  13. doi request reprint Maximum saccharification of cellulose complex by an enzyme cocktail supplemented with cellulase from newly isolated Aspergillus fumigatus ECU0811
    Dan Wang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, China
    Appl Biochem Biotechnol 166:176-86. 2012
    ..2 FPU CPCel plus 1.21 FPU AFCel per gram of glucan). On the other side, it reduced the total protein amount of CPCel by as much as tenfold, which extremely improved the hydrolytic rate of Spezyme CP and reduced its dosage...
  14. doi request reprint Efficient synthesis of a chiral precursor for angiotensin-converting enzyme (ACE) inhibitors in high space-time yield by a new reductase without external cofactors
    Nai Dong Shen
    Laboratory of Biocatalysis and Synthetic Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Org Lett 14:1982-5. 2012
    ..For the first time, (R)-HPBE with >99% ee was produced via bioreduction of OPBE at 1 M without external addition of cofactors. The space-time yield (700 g·L(-1)·d(-1)) was 27 times higher than the highest record...
  15. doi request reprint Bioproduction of glycolic acid from glycolonitrile with a new bacterial isolate of Alcaligenes sp. ECU0401
    Yu Cai He
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People s Republic of China
    Appl Biochem Biotechnol 160:1428-40. 2010
    ..2 g glycolic acid per gram dry cell weight after 29 batch recycles...
  16. doi request reprint Target-oriented discovery of a new esterase-producing strain Enterobacter sp. ECU1107 for whole cell-catalyzed production of (2S,3R)-3-phenylglycidate as a chiral synthon of Taxol
    Dong Jie Zhou
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Appl Microbiol Biotechnol 97:6293-300. 2013
    ..ECU1107, (2S,3R)-PGM was successfully prepared in decagram scale in a 1.0-l mechanically stirred reactor, affording the chiral epoxy ester in >99 % ee s and 43.5 % molar yield based on the initial load of racemic substrate...
  17. doi request reprint Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst
    Hui Lei Yu
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China
    J Biotechnol 133:469-77. 2008
    ..The productivity of the bioreactor in the case of salidroside can reach ca. 1.9 gl(-1)d(-1), affording the product in up to 99.3% purity after refinement...
  18. doi request reprint Highly stereoselective reduction of prochiral ketones by a bacterial reductase coupled with cofactor regeneration
    Yan Ni
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
    Org Biomol Chem 9:5463-8. 2011
    ..7% with enantiomeric purity of 99.6% ee...
  19. doi request reprint Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity
    Yan Ni
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Appl Microbiol Biotechnol 89:1111-8. 2011
    ..used as biocatalyst for stereoselective reduction of carbonyl compounds. This study provides a useful guidance for further application of this enzyme in the asymmetric synthesis of chiral alcohol enantiomers...
  20. doi request reprint Sequence analysis and heterologous expression of a new cytochrome P450 monooxygenase from Rhodococcus sp. for asymmetric sulfoxidation
    Jian Dong Zhang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People s Republic of China
    Appl Microbiol Biotechnol 85:615-24. 2010
    ..which is responsible for the asymmetric oxidation of sulfides. The new enzymatic activity of P450SMO on the above compounds makes it an attractive biocatalyst for asymmetric synthesis of enantiopure sulfoxides...
  21. doi request reprint Bioproduction of chiral mandelate by enantioselective deacylation of alpha-acetoxyphenylacetic acid using whole cells of newly isolated Pseudomonas sp. ECU1011
    Xin Ju
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People s Republic of China
    Appl Microbiol Biotechnol 86:83-91. 2010
    ..Furthermore, using this newly isolated strain of Pseudomonas sp. ECU1011, three kinds of optically pure analogs of (S)-mandelic acid and (R)-alpha-acetoxyphenylacetic acid were successfully prepared at high enantiomeric purity...
  22. doi request reprint Efficient production of (S)-naproxen with (R)-substrate recycling using an overexpressed carboxylesterase BsE-NP01
    Xiang Liu
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, China
    Appl Biochem Biotechnol 162:1574-84. 2010
    ..Batch production of (S)-naproxen was repeatedly carried out in a solid-water biphasic system at 2-L scale, achieving an average total yield of about 85% after ten runs with complete recycling of (R)-substrate...
  23. doi request reprint Improved expression of recombinant cytochrome P450 monooxygenase in Escherichia coli for asymmetric oxidation of sulfides
    Jian Dong Zhang
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, People s Republic of China
    Bioprocess Biosyst Eng 33:1043-9. 2010
    ..With p-chlorothioanisole as a representative substrate, the desired product (S-sulfoxide) was afforded with 99% ee and highest production of 130 mg/L within 12 h...
  24. doi request reprint Enzymatic production of Cilastatin intermediate via highly enantioselective hydrolysis of methyl (±)-2,2-dimethylcyclopropane carboxylate using newly isolated Rhodococcus sp. ECU1013
    Chao hong Liu
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People s Republic of China
    Appl Microbiol Biotechnol 97:7659-67. 2013
    ..As a result, (±)-DMCPM of up to 400 mM in the organic phase of isooctane was enantioselectively hydrolyzed into (S)-(+)-DMCPA, with an isolation yield of 38 % and a further increase of ee p to 99 %. ..
  25. ncbi request reprint Optimization of Serratia marcescens lipase production for enantioselective hydrolysis of 3-phenylglycidic acid ester
    Li Gao
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China
    J Ind Microbiol Biotechnol 31:525-30. 2004
    ..The resin-8244-lipase preparation can be used for efficient enantioselective hydrolysis of trans-3-(4'-methoxyphenyl)glycidic acid methyl ester [(+/-)-MPGM], a key intermediate in the synthesis of Diltiazem...
  26. ncbi request reprint Improved catalytic performance of Bacillus megaterium epoxide hydrolase in a medium containing Tween-80
    Peng Fei Gong
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P R China
    Biotechnol Prog 19:652-4. 2003
    ..5 times. The enzymatic hydrolysis of rac-GPE was highly enantioselective, with an E-value (enantiomeric ratio) of 69.3 in the Tween-80 emulsion system, which is obviously higher than that (41.2) observed in the DMSO-containing system...
  27. ncbi request reprint Isolation of lipase producer and its performance in enantioselective hydrolysis of glycidyl butyrate
    Shi Ying Jia
    Laboratory of Biocatalysis, PO Box 283, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P R China
    Appl Biochem Biotechnol 104:69-79. 2003
    ..3 and 42 degrees C. Higher enantioselectivity of the enzyme was observed at lower temperatures, while the best enantioselectivity was obtained at pH 5.5-6.0, with an E value (enantiomeric ratio) of 57...
  28. doi request reprint Stereocomplementary bioreduction of β-ketonitrile without ethylated byproduct
    Guo Chao Xu
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Org Lett 15:5408-11. 2013
    ..For the first time, both enantiomers of fluoroxetine precursors were obtained at 0.5 M with >99% ee and excellent chemoselectivity, without addition of any external cofactors. ..
  29. doi request reprint Crystal structures of Pseudomonas putida esterase reveal the functional role of residues 187 and 287 in substrate binding and chiral recognition
    Shuai Dou
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
    Biochem Biophys Res Commun 446:1145-50. 2014
    ....
  30. doi request reprint A new thermostable β-glucosidase mined from Dictyoglomus thermophilum: properties and performance in octyl glucoside synthesis at high temperatures
    Zheng Zheng Zou
    Laboratory of Biocatalysis and Synthetic Biotechnology, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 118:425-30. 2012
    ..It could retain part of synthetic activity even in boiling water. Owing to the strong glucose-tolerance and extremely high thermostability, DtGH should be promising for various glucosides synthesis...
  31. doi request reprint An efficient bioprocess for enzymatic production of L-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli
    Gao Wei Zheng
    Laboratory of Biocatalysis and Bioprocessing, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China
    J Biotechnol 150:108-14. 2010
    ..Moreover, the substrate loading was increased up to 200 g L(-1) (∼1 M), the biocatalyst loading was reduced to 2.5 g L(-1) and the space-time yield was improved from 54 g L(-1) d(-1) to 202 g L(-1) d(-1)...
  32. doi request reprint Isolation of a Bacillus strain producing ketone reductase with high substrate tolerance
    Yan Xie
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 101:1054-9. 2010
    ..e. values. These results suggest the potential of this strain for the industrial production of chiral halogenated aromatic alcohols...
  33. doi request reprint Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system
    Zhi Jun Zhang
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    J Biotechnol 152:24-9. 2011
    ..7g (R)-(-)-mandelic acid in 98.0% ee (enantiomeric excess) and a specific production of 13.8g (mandelic acid) g(-1) (cell), respectively...
  34. ncbi request reprint Enantioconvergent hydrolysis of styrene epoxides by newly discovered epoxide hydrolases in mung bean
    Wei Xu
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Org Lett 8:1737-40. 2006
    ..7% overall yield (after recrystallization)...
  35. doi request reprint Thermostable bacterial endoglucanases mined from SWISS-PROT database
    Li Huan Qiu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 165:1473-84. 2011
    ..All these features, especially the excellent thermostability of EGtf1 and EGtf2, enable them to become a good candidate for further protein engineering to realize the ultimate practical application in biomass industry...
  36. doi request reprint Enantioselective hydrolysis of dl-menthyl benzoate by cell-free extract of newly isolated Acinetobacter sp. ECU2040
    Minh Thu Ngo-Thi
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People s Republic of China
    Appl Biochem Biotechnol 170:1974-81. 2013
    ..5 and 37 °C, respectively. Under the optimal conditions, the enzymatic reaction was performed on a 1-L scale, affording l-menthol in 48 % yield and 71 % ee...
  37. doi request reprint Isolation and biochemical characterization of two novel metagenome-derived esterases
    Li Ming Ouyang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 169:15-28. 2013
    ..5, respectively, and the enzyme exhibited strong tolerance in the presence of 30 % methanol. EstF4K and EstP2K showed opposite enantioselectivity for methyl 3-phenylglycidate, a chiral synthon for the synthesis of Taxol® side chain...
  38. doi request reprint Improved production of Pseudomonas sp. ECU1011 acetyl esterase by medium design and fed-batch fermentation
    Xin Ju
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, People s Republic of China
    Bioprocess Biosyst Eng 35:323-31. 2012
    ..2-fold higher than the initial) without enantioselectivity decline. This process has potential applications for industrial production of chiral mandelic acid or its derivatives...
  39. doi request reprint Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase
    Zhi Jun Zhang
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China
    Bioprocess Biosyst Eng 37:1241-8. 2014
    ..Therefore, the easy preparation and robust characteristics of the immobilized biocatalyst make it a very promising biocatalyst for high-performance and low-cost production of optically pure (R)-(-)-mandelic acid...
  40. ncbi request reprint Improved production of ( S)-ketoprofen ester hydrolase by a mutant of Trichosporon brassicae CGMCC 0574
    Hui Yuan Wu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, P R China
    J Ind Microbiol Biotechnol 30:357-61. 2003
    ..An 18-h culture grown on a medium containing 0.5% glucose plus 0.5% isopropanol produced 3-fold as much esterase as a culture grown on 1% glucose...
  41. doi request reprint A new chemo-enzymatic route to chiral 2-hydroxy-4-phenylbutyrates by combining lactonase-mediated resolution with hydrogenation over Pd/C
    Bing Chen
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China
    Chem Commun (Camb) 46:2754-6. 2010
    ..The key step is the lactonase-catalyzed hydrolysis of cis- and trans-2-hydroxy-4-phenyl-4-butyrolactones followed by hydrogenation over Pd/C to afford optically pure 2-hydroxy-4-phenylbutyric acid...
  42. doi request reprint Combinatorial evolution of phosphotriesterase toward a robust malathion degrader by hierarchical iteration mutagenesis
    Xiao Jing Luo
    State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237, China
    Biotechnol Bioeng 113:2350-7. 2016
    ..6°C. Thus, PoOPHM9 appears to be an efficient and robust candidate for malathion detoxification. Biotechnol. Bioeng. 2016;113: 2350-2357. © 2016 Wiley Periodicals, Inc. ..
  43. doi request reprint Improved efficiency of a novel methyl parathion hydrolase using consensus approach
    Xu Yun Liu
    State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, China The National Engineering Research Center for Miniaturized Detection Systems, Xi an 710069, China
    Enzyme Microb Technol 93:11-17. 2016
    ..This study generated an efficient MP-degrading enzyme, and provides useful information for enhancing the catalytic efficiency of MPHs via conservative residue substitution based on the consensus approach...
  44. doi request reprint Switching a newly discovered lactonase into an efficient and thermostable phosphotriesterase by simple double mutations His250Ile/Ile263Trp
    Xiao Jing Luo
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
    Biotechnol Bioeng 111:1920-30. 2014
    ..0 U mg(-1) ) toward methyl-parathion. Our results offer a first scrutiny to PTE activity evolution of OPHs in β-lactamase superfamily and provide efficient and robust enzymes for OP detoxification...
  45. doi request reprint Discovery and utilization of biocatalysts for chiral synthesis: an overview of Chinese scientists' research and development
    Hui Lei Yu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China
    Adv Biochem Eng Biotechnol 113:1-31. 2009
    ....
  46. doi request reprint Synthesis of novel salidroside esters by lipase-mediated acylation with various functional acyl groups
    Hui Lei Yu
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    J Biosci Bioeng 106:65-8. 2008
    ....
  47. doi request reprint New opportunities for biocatalysis: driving the synthesis of chiral chemicals
    Gao Wei Zheng
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Curr Opin Biotechnol 22:784-92. 2011
    ..In this review, new opportunities for biocatalysis in the preparation of chiral molecules are outlined and highlighted...
  48. doi request reprint Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by cetyltrimethylammonium bromide-permeabilized cells of Alcaligenes faecalis ECU0401
    Yu Cai He
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    J Ind Microbiol Biotechnol 37:741-50. 2010
    ..Efficient biocatalyst recycling was achieved as a result of cell immobilization in calcium alginate, with a product-to-biocatalyst ratio of 3.82 g (R)-(-)-mandelic acid g(-1) dry cell weight (dcw) cell after 20 cycles of repeated use...
  49. doi request reprint Biocatalytic properties of a recombinant Fusarium proliferatum lactonase with significantly enhanced production by optimal expression in Escherichia coli
    Bing Chen
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 162:744-56. 2010
    ..The results reported in this paper provide a feasible method to make the whole cells of E. coli JM109 (DE3) expressing lactonase gene to be a highly efficient and easy-to-make biocatalyst for asymmetric synthesis of chiral compounds...
  50. doi request reprint Rational design of a carboxylic esterase RhEst1 based on computational analysis of substrate binding
    Qi Chen
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
    J Mol Graph Model 62:319-24. 2015
    ..This work provides molecular-level insights into the substrate binding mechanism of carboxylic esterase RhEst1, facilitating future experimental efforts toward developing more efficient RhEst1 variants for industrial applications. ..
  51. doi request reprint Highly efficient synthesis of (R)-3-quinuclidinol in a space-time yield of 916 g L(-1) d(-1) using a new bacterial reductase ArQR
    Wen Xia Zhang
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Org Lett 15:4917-9. 2013
    ..This is the first time that a high yield of (R)-3-quinuclidinol up to 916 g L(-1) d(-1) using a bioreduction approach is reported. ..
  52. doi request reprint Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol
    Qing Qing Zhu
    State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People s Republic of China
    Appl Microbiol Biotechnol 98:207-18. 2014
    ....
  53. doi request reprint Biocatalytic ketone reduction: a green and efficient access to enantiopure alcohols
    Yan Ni
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Biotechnol Adv 30:1279-88. 2012
    ..In addition, the integration of bioreduction with other enzymatic or chemical steps allows the efficient synthesis of more complex chiral products...
  54. doi request reprint Efficient biosynthesis of uridine diphosphate glucose from maltodextrin by multiple enzymes immobilized on magnetic nanoparticles
    Qing Dong
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, PR China
    Carbohydr Res 345:1622-6. 2010
    ..After a simple purification, approximately 630 mg of crystallized UDP-Glc was obtained from 1 l of reaction mixture, for a moderate yield of around 50% (UTP conversion) at very low cost...
  55. doi request reprint Catalytic performance of corn stover hydrolysis by a new isolate Penicillium sp. ECU0913 producing both cellulase and xylanase
    Qian Qian Shi
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People s Republic of China
    Appl Biochem Biotechnol 164:819-30. 2011
    ..With an enzyme dosage of 50 FPU/g dry substrate, the conversions of cellulose and hemicellulose reached 77.2% and 47.5%, respectively, without adding any accessory enzyme...
  56. doi request reprint Cloning and Characterization of an Enantioselective l-Menthyl Benzoate Hydrolase from Acinetobacter sp. ECU2040
    Jin Gang Yin
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
    Appl Biochem Biotechnol 176:1102-13. 2015
    ..5) towards dl-menthyl benzoate. It can also catalyze the enantioselective hydrolysis of a variety of racemic menthyl esters, including dl-menthyl acetate, dl-menthyl chloroacetate, and dl-menthyl butyrate. ..
  57. doi request reprint Efficient Biocatalytic Synthesis of Chiral Chemicals
    Zhi Jun Zhang
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China
    Adv Biochem Eng Biotechnol 155:55-106. 2016
    ....
  58. pmc Cloning and Characterization of a Novel Esterase from Rhodococcus sp. for Highly Enantioselective Synthesis of a Chiral Cilastatin Precursor
    Yan Zhang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
    Appl Environ Microbiol 80:7348-55. 2014
    ..8% and an enantiomeric excess (ee) of 97.5%, indicating an extremely high enantioselectivity (E = 240) of this novel and unique biocatalyst for green manufacturing of highly valuable chiral chemicals. ..
  59. doi request reprint A novel D-mandelate dehydrogenase used in three-enzyme cascade reaction for highly efficient synthesis of non-natural chiral amino acids
    Chang Wei Fan
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    J Biotechnol 195:67-71. 2015
    ..Under the optimum conditions, 30.4 g rac-mandelic acid (0.2 M) at 1L scale had been converted into chiral L-phenylglycine, with 96.4% conversion, 86.5% isolation yield, >99% eep and 50.4 gL(-1)d(-1) space-time yield...
  60. doi request reprint Performance of a new thermostable mannanase in breaking guar-based fracturing fluids at high temperatures with little premature degradation
    Ke Hu
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
    Appl Biochem Biotechnol 172:1215-26. 2014
    ..4 mg L(-1) of the residue was left after the enzymatic reaction. All these positive features demonstrate the great potential of this mannanase as a new enzyme breaker for application in enhanced recovery of petroleum oil...
  61. doi request reprint An ene reductase from Clavispora lusitaniae for asymmetric reduction of activated alkenes
    Yan Ni
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Enzyme Microb Technol 56:40-5. 2014
    ..All these positive features demonstrate a high synthetic potential of ClER in the asymmetric reduction of activated alkenes...
  62. doi request reprint Improving enzymatic production of ginsenoside Rh2 from Rg3 by using nonionic surfactant
    Jin Huan Su
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 160:1116-23. 2010
    ..5%, w/v), which was 25% higher than that in buffer without any surfactant. Furthermore, the enzyme stability was affected faintly by the surfactant...
  63. doi request reprint Adzuki bean: a new resource of biocatalyst for asymmetric reduction of aromatic ketones with high stereoselectivity and substrate tolerance
    Yan Xie
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 100:2463-8. 2009
    ..g. 100mM), exhibiting excellent stereoselectivity (>98% e.e.). In addition, it was found that NADPH acts as the reducing cofactor, which can be regenerated by the crude enzyme system itself using glucose as an auxiliary substrate...
  64. doi request reprint Significantly enhanced production of recombinant nitrilase by optimization of culture conditions and glycerol feeding
    Jun Feng Liu
    East China University of Science and Technology, Shanghai, People s Republic of China
    Appl Microbiol Biotechnol 89:665-72. 2011
    ..Finally, under the optimized conditions for fed-batch culture, cell growth, specific activity and nitrilase production of the recombinant E. coli were increased by 9.0-, 5.5-, and 50-fold, respectively...
  65. doi request reprint Significantly improved thermostability of a reductase CgKR1 from Candida glabrata with a key mutation at Asp 138 for enhancing bioreduction of aromatic α-keto esters
    Lei Huang
    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
    J Biotechnol 203:54-61. 2015
    ....
  66. pmc Engineering of an epoxide hydrolase for efficient bioresolution of bulky pharmaco substrates
    Xu Dong Kong
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China State Key Laboratory of Bio organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China and
    Proc Natl Acad Sci U S A 111:15717-22. 2014
    ..These results show great promise for structure-based rational design in improving the catalytic efficiency of industrial enzymes for bulky substrates. ..
  67. doi request reprint Double substituted variant of Bacillus amyloliquefaciens esterase with enhanced enantioselectivity and high activity towards 1-(3',4'-methylenedioxyphenyl)ethyl acetate
    Jia Yan Liu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China
    Appl Microbiol Biotechnol 99:1701-8. 2015
    ....
  68. doi request reprint A smart library of epoxide hydrolase variants and the top hits for synthesis of (S)-β-blocker precursors
    Xu Dong Kong
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237 China
    Angew Chem Int Ed Engl 53:6641-4. 2014
    ..This study represents a breakthrough in protein engineering of epoxide hydrolases and resulted in enhanced activity toward bulky substrates. ..
  69. doi request reprint Highly efficient synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate and its derivatives by a robust NADH-dependent reductase from E. coli CCZU-K14
    Yu Cai He
    Laboratory of Biocatalysis and Bioprocessing, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou 213164, PR China State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China Electronic address
    Bioresour Technol 161:461-4. 2014
    ..0%) and high enantiometric excess value (>99.9% ee) after 14h. Significantly, E. coli CCZU-K14 shows high potential in the industrial production of (S)-CHBE and its derivatives (>99.9% ee). ..
  70. ncbi request reprint Significant improvement of Serratia marcescens lipase fermentation, by optimizing medium, induction, and oxygen supply
    Zhang De Long
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People s Republic of China
    Appl Biochem Biotechnol 142:148-57. 2007
    ....
  71. doi request reprint Highly selective and controllable synthesis of arylhydroxylamines by the reduction of nitroarenes with an electron-withdrawing group using a new nitroreductase BaNTR1
    Hieu Huy Nguyen-Tran
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
    Chem Commun (Camb) 50:2861-4. 2014
    ..This method therefore represents a green and efficient method for the synthesis of arylhydroxylamines. ..
  72. doi request reprint Essential role of Gly33 in a novel organic solvent-tolerant lipase from Serratia marcescens ECU1010 as determined by site-directed mutagenesis
    Su Xia Li
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People s Republic of China
    Appl Biochem Biotechnol 172:2945-54. 2014
    ..The replacement of Gly at position 33 by Asp obviously decreased its stability in organic solvents. Molecular modeling and structural analysis also suggested that the Gly33 residue is important for the organic solvent stability of lipB. ..
  73. ncbi request reprint Preparation of (R)-(-)-mandelic acid and its derivatives from racemates by enantioselective degradation with a newly isolated bacterial strain Alcaligenes sp. ECU0401
    Yu Cai He
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People s Republic of China
    Bioprocess Biosyst Eng 31:445-51. 2008
    ..e. > 99.9%). (R)-(-)-Mandelic acid could also be obtained after 12 h of biotransformation with 41.5% isolated yield and >99.9% e.e...
  74. pmc Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol
    Xiang Liu
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    J Biotechnol 164:188-95. 2012
    ....
  75. pmc Isolation of Rhodococcus sp. strain ECU0066, a new sulfide monooxygenase-producing strain for asymmetric sulfoxidation
    Ai Tao Li
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
    Appl Environ Microbiol 75:551-6. 2009
    ..This bacterial strain also displayed fairly good activity and enantioselectivity toward seven other sulfides, indicating a good potential for practical application in asymmetric synthesis of chiral sulfoxides...
  76. ncbi request reprint Biocatalytic resolution of glycidyl aryl ethers by Trichosporon loubierii : cell/substrate ratio influences the optical purity of (R) - epoxides
    Yi Xu
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P R China
    Biotechnol Lett 26:1217-21. 2004
    ..An additional stability test of the whole-cell enzyme suggests that rapid deactivation of the epoxide hydrolase was the potential reason. (R)-Epoxides were prepared in gram amounts with optical purity of 87% - 99% ee...
  77. ncbi request reprint Integration of purification with immobilization of Candida rugosa lipase for kinetic resolution of racemic ketoprofen
    You Yan Liu
    College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China
    J Biotechnol 110:209-17. 2004
    ..Under optimal conditions, the air-bubbled column reactor could be operated smoothly for at least 350 h, remaining nearly 50% activity...
  78. ncbi request reprint Investigating pH and Cu (II) effects on lipase activity and enantioselectivity via kinetic and spectroscopic methods
    Tian Wen Xu
    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, Shanghai, PR China
    Biotechnol J 1:1293-301. 2006
    ....