Alexander N Zakhartchouk

Summary

Affiliation: University of Saskatchewan
Country: Canada

Publications

  1. ncbi request reprint Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice
    Alexander N Zakhartchouk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3
    J Gen Virol 86:211-5. 2005
  2. ncbi request reprint Augmentation of immune responses to SARS coronavirus by a combination of DNA and whole killed virus vaccines
    Alexander N Zakhartchouk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Sask, Canada S7N 5E3
    Vaccine 23:4385-91. 2005
  3. ncbi request reprint Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice: implications for a subunit vaccine
    Alexander N Zakhartchouk
    Vaccine and Infectious Disease Organization VIDO, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Sask, Canada S7N 5E3
    Vaccine 25:136-43. 2007
  4. ncbi request reprint Optimization of a DNA vaccine against SARS
    Alexander N Zakhartchouk
    Vaccine and Infectious Disease Organization VIDO, University of Saskatchewan, Saskatoon, SK, Canada
    DNA Cell Biol 26:721-6. 2007
  5. ncbi request reprint Intracellular localization of the SARS coronavirus protein 9b: evidence of active export from the nucleus
    Igor Moshynskyy
    Vaccine and Infectious Disease Organization VIDO, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    Virus Res 127:116-21. 2007
  6. doi request reprint The recombinant globular head domain of the measles virus hemagglutinin protein as a subunit vaccine against measles
    Liubov M Lobanova
    Vaccine and Infectious Disease Organization International Vaccine Center, University of Saskatchewan, Saskatoon, SK, S7N 5E3 Canada
    Vaccine 30:3061-7. 2012
  7. doi request reprint Mucosal adenovirus-vectored vaccine for measles
    Liubov M Lobanova
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, S7N 5E3 Canada
    Vaccine 28:7613-9. 2010
  8. pmc Potential role of porcine reproductive and respiratory syndrome virus structural protein GP2 in apoptosis inhibition
    Sujit Pujhari
    International Vaccine Center VIDO InterVac, Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3
    Biomed Res Int 2014:160505. 2014
  9. doi request reprint Role of phosphatidylinositol-3-kinase (PI3K) and the mammalian target of rapamycin (mTOR) signalling pathways in porcine reproductive and respiratory syndrome virus (PRRSV) replication
    Sujit Pujhari
    Vaccine and Infectious Disease Organization, International Vaccine Center VIDO InterVac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, S7N 5E3 Canada
    Virus Res 194:138-44. 2014
  10. doi request reprint Development of a DNA-launched replicon as a vaccine for porcine reproductive and respiratory syndrome virus
    Sujit Pujhari
    Vaccine and Infectious Disease Organization International Vaccine Center VIDO InterVac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    Virus Res 173:321-6. 2013

Collaborators

Detail Information

Publications12

  1. ncbi request reprint Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice
    Alexander N Zakhartchouk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3
    J Gen Virol 86:211-5. 2005
    ..Vaccination of C57BL/6 mice with Ad5-N-V generated potent SARS-CoV-specific humoral and T cell-mediated immune responses. These results show that Ad5-N-V may potentially be used as a SARS-CoV vaccine...
  2. ncbi request reprint Augmentation of immune responses to SARS coronavirus by a combination of DNA and whole killed virus vaccines
    Alexander N Zakhartchouk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Sask, Canada S7N 5E3
    Vaccine 23:4385-91. 2005
    ..These results demonstrate that combination of the DNA vaccine and the whole killed virus vaccine can be used to enhance the magnitude and change the bias of the immune responses to SARS-CoV...
  3. ncbi request reprint Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice: implications for a subunit vaccine
    Alexander N Zakhartchouk
    Vaccine and Infectious Disease Organization VIDO, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Sask, Canada S7N 5E3
    Vaccine 25:136-43. 2007
    ..This finding suggests that carbohydrate is important for the immunogenicity of the S318-510 protein fragment and provide useful information for designing an effective and safe SARS subunit vaccine...
  4. ncbi request reprint Optimization of a DNA vaccine against SARS
    Alexander N Zakhartchouk
    Vaccine and Infectious Disease Organization VIDO, University of Saskatchewan, Saskatoon, SK, Canada
    DNA Cell Biol 26:721-6. 2007
    ..Analysis of humoral and cellular immune responses in immunized mice demonstrated that pcDNA-SS and pcDNA-St-VP22C are the most immunogenic SARS vaccine candidates...
  5. ncbi request reprint Intracellular localization of the SARS coronavirus protein 9b: evidence of active export from the nucleus
    Igor Moshynskyy
    Vaccine and Infectious Disease Organization VIDO, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    Virus Res 127:116-21. 2007
    ..Our data also suggest that the 46-LRLGSQLSL-54 amino acid sequence of 9b functions as a nuclear export signal (NES)...
  6. doi request reprint The recombinant globular head domain of the measles virus hemagglutinin protein as a subunit vaccine against measles
    Liubov M Lobanova
    Vaccine and Infectious Disease Organization International Vaccine Center, University of Saskatchewan, Saskatoon, SK, S7N 5E3 Canada
    Vaccine 30:3061-7. 2012
    ..Furthermore, the inclusion of polyphosphazene into the vaccine formulation induced a mixed Th1/Th2-type immune response. In addition, the purified recombinant protein retained its immunogenicity even after storage at 37°C for 2 weeks...
  7. doi request reprint Mucosal adenovirus-vectored vaccine for measles
    Liubov M Lobanova
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, S7N 5E3 Canada
    Vaccine 28:7613-9. 2010
    ..Moreover, Ad-F/H vaccine administered intranasally, but not intramuscularly, completely protected challenged cotton rats from MV replication in the lungs...
  8. pmc Potential role of porcine reproductive and respiratory syndrome virus structural protein GP2 in apoptosis inhibition
    Sujit Pujhari
    International Vaccine Center VIDO InterVac, Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3
    Biomed Res Int 2014:160505. 2014
    ..Overall, our data suggest that, although both stable cell lines activate NF- κ B and AP-1, GP2 triggers the antiapoptotic process through an intermediate step that needs to be further investigated. ..
  9. doi request reprint Role of phosphatidylinositol-3-kinase (PI3K) and the mammalian target of rapamycin (mTOR) signalling pathways in porcine reproductive and respiratory syndrome virus (PRRSV) replication
    Sujit Pujhari
    Vaccine and Infectious Disease Organization, International Vaccine Center VIDO InterVac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, S7N 5E3 Canada
    Virus Res 194:138-44. 2014
    ..In this article, we will review the role of these signalling pathways during PRRSV replication, and discuss some of our recent findings implicating mTOR. ..
  10. doi request reprint Development of a DNA-launched replicon as a vaccine for porcine reproductive and respiratory syndrome virus
    Sujit Pujhari
    Vaccine and Infectious Disease Organization International Vaccine Center VIDO InterVac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    Virus Res 173:321-6. 2013
    ..We demonstrated the feasibility of this approach by expression of a foreign gene encoding firefly luciferase after transfection of cultured cells with the replicon plasmid DNA...
  11. ncbi request reprint Construction of capsid-modified recombinant bovine adenovirus type 3
    Alexander N Zakhartchouk
    Vectored Vaccine Program VIDO, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    Methods Mol Med 130:91-106. 2007
    ..g., fiber, pIX) has helped in increasing the utility of BAdV-3 as a vector for transducing nonbovine cells. Here, we will describe the methods used to construct recombinant BAdV-3 expressing chimeric fiber or chimeric pIX proteins...
  12. ncbi request reprint Comparative evaluation of two severe acute respiratory syndrome (SARS) vaccine candidates in mice challenged with SARS coronavirus
    Raymond H See
    University of British Columbia Centre for Disease Control, Vancouver, BC V5Z 4R4, Canada
    J Gen Virol 87:641-50. 2006
    ..Finally, the sera of vaccinated mice contained antibodies to S, further suggesting a role for this protein in conferring protective immunity against SARS-CoV infection...