Jim Hu

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. pmc Tolerogenic dendritic cells and their potential applications
    Jim Hu
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada
    Immunology 132:307-14. 2011
  2. pmc Induction of immunological tolerance to adenoviral vectors by using a novel dendritic cell-based strategy
    Rahul Kushwah
    Address correspondence to Jim Hu
    J Virol 86:3422-35. 2012
  3. pmc Role of dendritic cells in the induction of regulatory T cells
    Rahul Kushwah
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    Cell Biosci 1:20. 2011
  4. pmc Subretinal gene delivery using helper-dependent adenoviral vectors
    Linda Wu
    Physiology and Experimental Medicine Program, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G1X8, Canada
    Cell Biosci 1:15. 2011
  5. pmc Regulation of epithelium-specific Ets-like factors ESE-1 and ESE-3 in airway epithelial cells: potential roles in airway inflammation
    Jing Wu
    Department of Physiology and Experimental Medicine and Canadian Institutes of Health Research Group in Lung Development, The Hospital for Sick Children, The University of Toronto, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Cell Res 18:649-63. 2008
  6. pmc Activation of P2X(7) receptor by ATP plays an important role in regulating inflammatory responses during acute viral infection
    Benjamin H Lee
    Research Institute, The Hospital for Sick Children, Toronto, Canada
    PLoS ONE 7:e35812. 2012
  7. ncbi request reprint Characterization of pulmonary T cell response to helper-dependent adenoviral vectors following intranasal delivery
    Rahul Kushwah
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
    J Immunol 180:4098-108. 2008
  8. doi request reprint Elf3 plays a role in regulating bronchiolar epithelial repair kinetics following Clara cell-specific injury
    Jordan R Oliver
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
    Lab Invest 91:1514-29. 2011
  9. pmc Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig
    Jonathan C Yeung
    Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University of Toronto, Toronto, Ontario, Canada
    Mol Ther 20:1204-11. 2012
  10. doi request reprint Adenoviral vectors stimulate innate immune responses in macrophages through cross-talk with epithelial cells
    Benjamin H Lee
    Physiology and Experimental Medicine Program, Toronto, Ontario, Canada
    Immunol Lett 134:93-102. 2010

Collaborators

Detail Information

Publications40

  1. pmc Tolerogenic dendritic cells and their potential applications
    Jim Hu
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada
    Immunology 132:307-14. 2011
    ..Furthermore, most of the information on tolerogenic DCs has been obtained from animal models and translational studies are needed to examine how a DC therapeutic strategy can be implemented clinically to modulate immunity...
  2. pmc Induction of immunological tolerance to adenoviral vectors by using a novel dendritic cell-based strategy
    Rahul Kushwah
    Address correspondence to Jim Hu
    J Virol 86:3422-35. 2012
    ..Taken together, these studies identify the use of DCs generated in the presence of IL-10 as a novel strategy to induce long-lasting immune tolerance to HD-Ad vectors...
  3. pmc Role of dendritic cells in the induction of regulatory T cells
    Rahul Kushwah
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    Cell Biosci 1:20. 2011
    ..In this review we discuss the recent advances in our understanding of the mechanisms underlying DC driven regulatory T cell induction...
  4. pmc Subretinal gene delivery using helper-dependent adenoviral vectors
    Linda Wu
    Physiology and Experimental Medicine Program, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G1X8, Canada
    Cell Biosci 1:15. 2011
    ..Since these vectors have a large cloning capacity, they have great potential to extend the success of gene therapy achieved using the adeno-associated viral vector...
  5. pmc Regulation of epithelium-specific Ets-like factors ESE-1 and ESE-3 in airway epithelial cells: potential roles in airway inflammation
    Jing Wu
    Department of Physiology and Experimental Medicine and Canadian Institutes of Health Research Group in Lung Development, The Hospital for Sick Children, The University of Toronto, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Cell Res 18:649-63. 2008
    ..Our findings suggest that ESE-1 and ESE-3 play an important role in airway inflammation...
  6. pmc Activation of P2X(7) receptor by ATP plays an important role in regulating inflammatory responses during acute viral infection
    Benjamin H Lee
    Research Institute, The Hospital for Sick Children, Toronto, Canada
    PLoS ONE 7:e35812. 2012
    ..These results indicate that ATP, released during viral infection, is an important inflammatory regulator that activates the inflammasome pathway and regulates inflammatory responses...
  7. ncbi request reprint Characterization of pulmonary T cell response to helper-dependent adenoviral vectors following intranasal delivery
    Rahul Kushwah
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
    J Immunol 180:4098-108. 2008
    ..These findings have implications for development of strategies to prevent adaptive immune responses against gene therapy vectors...
  8. doi request reprint Elf3 plays a role in regulating bronchiolar epithelial repair kinetics following Clara cell-specific injury
    Jordan R Oliver
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
    Lab Invest 91:1514-29. 2011
    ..Taken together, our results suggest that Elf3 plays an important role in the regulation of lung cell proliferation and differentiation during repair of the injured bronchiolar airway epithelium...
  9. pmc Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig
    Jonathan C Yeung
    Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University of Toronto, Toronto, Ontario, Canada
    Mol Ther 20:1204-11. 2012
    ..In summary, ex vivo delivery of adenoviral gene therapy to the donor lung is superior to in vivo delivery in that it leads to less vector-associated inflammation and provides superior post-transplant lung function...
  10. doi request reprint Adenoviral vectors stimulate innate immune responses in macrophages through cross-talk with epithelial cells
    Benjamin H Lee
    Physiology and Experimental Medicine Program, Toronto, Ontario, Canada
    Immunol Lett 134:93-102. 2010
    ..Furthermore, we show that only the macrophages adjacent to epithelial cells were activated during Ad infection demonstrating that the interaction between macrophages and epithelial cells are crucial for Ad-induced inflammatory response...
  11. pmc Uptake of apoptotic DC converts immature DC into tolerogenic DC that induce differentiation of Foxp3+ Treg
    Rahul Kushwah
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, Toronto, ON, Canada
    Eur J Immunol 40:1022-35. 2010
    ..Taken together, these findings identify uptake of apoptotic DC by viable immature DC as an immunologically tolerogenic event...
  12. pmc Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP
    Huibi Cao
    Physiology and Experimental Medicine Program, University of Toronto, Toronto, Canada
    PLoS ONE 6:e15860. 2011
    ..The second hypothesis suggests that these mutations have only a mild effect on the protein function and thus affect only the metabolically highly active cells such as retinal photoreceptors...
  13. doi request reprint Elf3 regulates allergic airway inflammation by controlling dendritic cell-driven T cell differentiation
    Rahul Kushwah
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Immunol 187:4639-53. 2011
    ..Taken together, our study identifies a novel function of Elf3 in regulating allergic airway inflammation by regulating DC-driven Th1, Th2, and Th17 differentiation...
  14. pmc Gene therapy: light is finally in the tunnel
    Huibi Cao
    Programme in Physiology and Experimental Medicine, Hospital for Sick Children, Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, M5G, 1X8, Canada
    Protein Cell 2:973-89. 2011
    ..In this article, we shall review the major advancements over the past two decades and, using lung gene therapy as an example, discuss the current obstacles and possible solutions to provide a roadmap for future gene therapy research...
  15. pmc Identification of a bone marrow-derived epithelial-like population capable of repopulating injured mouse airway epithelium
    Amy P Wong
    Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, and the McEwen Centre in Regenerative Medicine, Toronto, Ontario, Canada
    J Clin Invest 119:336-48. 2009
    ..Additionally, these data could reconcile previous controversies regarding the contribution of bone marrow to lung regeneration...
  16. doi request reprint Long-Term Expression of the Human CFTR Gene in Mouse Airway via Helper-Dependent Adenoviral Vector Delivery and Transient Immunosuppression
    Huibi Cao
    1 Program of Physiology and Experimental Medicine, University of Toronto, Toronto, Ontario, Canada
    Hum Gene Ther 27:83-91. 2016
    ..Our data suggest that transient immunosuppression might be a strategy to improve sustained expression in gene therapy. ..
  17. ncbi request reprint Potential of helper-dependent adenoviral vectors in modulating airway innate immunity
    Rahul Kushwah
    Physiology and Experimental Medicine Programme, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada, M5G1X8
    Cell Mol Immunol 4:81-9. 2007
    ..However, there still are a few limitations that need to be addressed prior to their use in clinical applications...
  18. doi request reprint Multiple roles of the epithelium-specific ETS transcription factor, ESE-1, in development and disease
    Jordan R Oliver
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Lab Invest 92:320-30. 2012
    ..Understanding the exact mechanisms by which ESE-1 regulates these processes can have important implications for the treatment of a wide range of diseases...
  19. pmc Apoptotic dendritic cells induce tolerance in mice through suppression of dendritic cell maturation and induction of antigen-specific regulatory T cells
    Rahul Kushwah
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, Toronto, ON M5G 1X8, Canada
    J Immunol 183:7104-18. 2009
    ..These findings demonstrate that apoptotic DCs are taken up by viable DCs in vivo, which promotes tolerance through suppression of DC maturation and induction of T(regs)...
  20. pmc POU homeodomain protein Oct-1 functions as a sensor for cyclic AMP
    Peixiang Wang
    Division of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, Toronto, Ontario M5G 1L7, Canada
    J Biol Chem 284:26456-65. 2009
    ..Our study therefore identifies a novel mechanism by which cAMP regulates hormone-gene expression and suggests that ubiquitously expressed Oct-1 may play a role in metabolic homeostasis by functioning as a sensor for cAMP...
  21. ncbi request reprint XB130, a novel adaptor protein for signal transduction
    Jing Xu
    Division of Cellular and Molecular Biology, University Health Network Toronto General Research Institute, and Hospital for Sick Children, Toronto, Ontario M5G 1L7, Canada
    J Biol Chem 282:16401-12. 2007
    ..These data suggest that XB130 as an adaptor may play an important role in the regulation of signal transduction and cellular functions...
  22. ncbi request reprint Aerosol delivery of an enhanced helper-dependent adenovirus formulation to rabbit lung using an intratracheal catheter
    David R Koehler
    Program in Lung Biology Research, The Hospital for Sick Children, Toronto, Canada M5G 1X8
    J Gene Med 7:1409-20. 2005
    ..Poor transduction of the ciliated airway epithelium and inefficient airway delivery of viral vectors are common difficulties encountered in lung gene therapy trials with large animals and humans...
  23. ncbi request reprint Targeted cell replacement with bone marrow cells for airway epithelial regeneration
    Amy P Wong
    Latner Thoracic Surgery Research Laboratories, McEwen Centre for Regenerative Medicine, Toronto Lung Transplant Program, Toronto General Hospital Research Institute, University Health Network, University of Toronto, Toronto, Canada
    Am J Physiol Lung Cell Mol Physiol 293:L740-52. 2007
    ..Some BMC cultured in vitro over time also expressed GFP transgene, suggesting epithelial transdifferentiation of the BMC. The results indicate that targeted delivery of BMC can promote airway regeneration...
  24. pmc Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference
    Hui Bi Cao
    Programme in Lung Biology Research and the Canadian Institutes of Health Research Group in Lung Development, Hospital for Sick Children, Toronto, Canada M5G 1X8
    Cell Res 15:111-9. 2005
    ....
  25. doi request reprint rbm47, a novel RNA binding protein, regulates zebrafish head development
    Rui Guan
    Zebrafish Centre for Advanced Drug Discovery, Keenan Research Centre, Li Ka Shing Knowledge Institute, St Michael s Hospital, Toronto, Ontario, Canada Department of Medicine, Physiology and Institute of Medical Science, University of Toronto, Canada Department of Laboratory Medicine and Pathology, University of Toronto and Program in Physiology and Experimental Medicine, The Hospital for Sick Children, Toronto, Canada Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital and Cancer Research Institute, Central South, Hunan Province, China
    Dev Dyn 242:1395-404. 2013
    ..RNA binding proteins play diverse roles in embryonic development and their roles in vertebrate head development remain to be elucidated...
  26. ncbi request reprint Adenoviral vectors for gene replacement therapy
    Huibi Cao
    Programme in Lung Biology Research and the Canadian Institutes of Health Research Group in Lung Development, Hospital for Sick Children, Toronto, Canada
    Viral Immunol 17:327-33. 2004
    ..Recent developments in vector design and delivery methods have improved the potential of Ads for successful gene therapy application...
  27. ncbi request reprint Lung inflammation as a therapeutic target in cystic fibrosis
    David R Koehler
    Programme in Lung Biology Research, Hospital for Sick Children, 555 University Ave, Toronto, ON, Canada M5G 1X8
    Am J Respir Cell Mol Biol 31:377-81. 2004
    ..Here we examine the mechanisms responsible for inflammation in the CF lung, and discuss potential therapeutic strategies targeting inflammation...
  28. pmc Epithelium-specific ets transcription factor 2 upregulates cytokeratin 18 expression in pulmonary epithelial cells through an interaction with cytokeratin 18 intron 1
    Deanna Yaniw
    Programme in Lung Biology Research and the Canadian Institutes of Health Research Group in Lung Development, Hospital for Sick Children, Toronto, Canada M5G 1X8
    Cell Res 15:423-9. 2005
    ..These experiments suggest that Ese-2 could play a role in the regulation of K18 expression in lung epithelial cells...
  29. pmc Alternative splicing of CD200 is regulated by an exonic splicing enhancer and SF2/ASF
    Zhiqi Chen
    Transplant Research Division, Department of Surgery and Immunology, The Toronto Hospital, University of Toronto, Toronto, Canada
    Nucleic Acids Res 38:6684-96. 2010
    ..Taken together, our data suggest for the first time that SF2/ASF regulates the function of CD200 by controlling CD200 alternative splicing, through direct binding to an ESE located in exon 2 of CD200...
  30. pmc Alternative polyadenylation of MeCP2: Influence of cis-acting elements and trans-acting factors
    Catherine M Newnham
    Department of Physiology and Experimental Medicine, University of Toronto, Toronto, ON, Canada
    RNA Biol 7:361-72. 2010
    ..In sum, the mechanism(s) of regulated alternative 3'UTR usage of MeCP2 are complex, and insight into these mechanisms will aid our understanding of the factors that influence MeCP2 expression...
  31. doi request reprint Dendritic cell apoptosis: regulation of tolerance versus immunity
    Rahul Kushwah
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children and Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    J Immunol 185:795-802. 2010
    ..This review discusses the regulatory pathways of DC apoptosis, stimuli inducing DC apoptosis, and the implications of DC apoptosis in the induction of immunosuppression and/or tolerance...
  32. pmc Complexity of dendritic cell subsets and their function in the host immune system
    Rahul Kushwah
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, Toronto, ON, Canada
    Immunology 133:409-19. 2011
    ..We classify DCs derived from common DC progenitor and pre-DC populations as conventional DCs, which includes both migratory and lymphoid-resident DC subsets and classify monocyte-derived DCs and plasmacytoid DCs as non-conventional DCs...
  33. pmc ESE-1 in Early Development: Approaches for the Future
    Chan Mi Lee
    Program in Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning, SickKids Research Institute, SickKids HospitalToronto, ON, Canada Laboratory Medicine and Pathobiology, University of TorontoToronto, ON, Canada
    Front Cell Dev Biol 4:73. 2016
    ..Understanding the role of ESE-1 in early development will provide new insights into its involvement in tissue regeneration and cancer, as well as how it functions with other Ets factors as a protein family. ..
  34. pmc Mutation in the splicing factor Hprp3p linked to retinitis pigmentosa impairs interactions within the U4/U6 snRNP complex
    Juana Maria Gonzalez-Santos
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, Toronto, ON, Canada
    Hum Mol Genet 17:225-39. 2008
    ....
  35. pmc Protection of Cftr knockout mice from acute lung infection by a helper-dependent adenoviral vector expressing Cftr in airway epithelia
    David R Koehler
    Programme in Lung Biology Research and Canadian Institutes of Health Research Group in Lung Development, Hospital for Sick Children, Toronto, ON, Canada M5G 1X8
    Proc Natl Acad Sci U S A 100:15364-9. 2003
    ..These data suggest that gene therapy could benefit cystic fibrosis patients by reducing susceptibility to opportunistic pathogens...
  36. pmc Epithelium-specific Ets transcription factor-1 acts as a negative regulator of cyclooxygenase-2 in human rheumatoid arthritis synovial fibroblasts
    Chan Mi Lee
    SickKids Research Institute, Program in Physiology and Experimental Medicine, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, 9th Floor, 686 Bay Street, Toronto, ON M5G 0A4 Canada Laboratory Medicine and Pathobiology, University of Toronto, 1 King s College Circle, Toronto, ON M5S 1A8 Canada
    Cell Biosci 6:43. 2016
    ..However, the role of ESE-1 in RA pathology has remained unclear. In this study, we aimed to elucidate the relationship between ESE-1 and COX-2 in RA synovial fibroblasts (RASFs) using a HD-Ad-mediated knockdown approach...
  37. doi request reprint Modulation of Alloimmune Responses by Interleukin-10 Prevents Rejection of Implanted Allogeneic Smooth Muscle Cells and Restores Postinfarction Ventricular Function
    Sanjiv Dhingra
    Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network and Department of Surgery, Division of Cardiac Surgery, University of Toronto, Toronto, Ontario, Canada
    Cell Transplant 24:1013-29. 2015
    ....
  38. doi request reprint GluA2 (GluR2) regulates metabotropic glutamate receptor-dependent long-term depression through N-cadherin-dependent and cofilin-mediated actin reorganization
    Zikai Zhou
    Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Neurosci 31:819-33. 2011
    ..These results provide compelling evidence that the extracellular domain of GluA2 regulates long-lasting synaptic plasticity through a signaling mechanism that is distinct from those used by the other domains of the receptor subunit...
  39. ncbi request reprint Expression of chicken ovalbumin upstream promoter-transcription factor II enhances invasiveness of human lung carcinoma cells
    Roya Navab
    Lung Biology Research Programme and Canadian Institutes of Health Research Group in Lung Development, The Hospital for Sick Children, 555 University Avenue, Montreal, Quebec, Canada M5G 1X8
    Cancer Res 64:5097-105. 2004
    ..These results suggest that COUP-TFII may be considered as a new target for anticancer therapies...
  40. ncbi request reprint Central region of the human splicing factor Hprp3p interacts with Hprp4p
    Juana Maria Gonzalez-Santos
    Program in Lung Biology Research, Hospital for Sick Children, Toronto, Ontario, Canada
    J Biol Chem 277:23764-72. 2002
    ....